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  • seminal plasma and spermatozoa
  • Semen was washed through 15% Percoll to remove seminal plasma and spermatozoa resuspended to 350 x 10(6)sperm/mL in a clear Hepes buffered diluent containing either 0, 5, 10, 20, 40 or 80% seminal plasma for 15 min, prior to being diluted to a final concentration of 50 x 10(6)sperm/mL in a Lactose-EDTA freezing diluent and cryopreserved. (biomedsearch.com)
  • Frontiers
  • Intratesticular signals regulate germ cell progression and production of qualitatively mature spermatozoa in vertebrates," Frontiers in Endocrinology , vol. 5, article 69, 2014. (hindawi.com)
  • 1997
  • Aitken RJ, Fisher HM, Fulton N, Gomez E, Knox W, Lewis B, Irvine S (1997) Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavoprotein inhibitors diphenylene iodonium and quinacrine. (springer.com)
  • male
  • Spermatozoa were prepared from semen collected by massage from a n adult male rhea. (docme.ru)
  • It can be used to sort out sperm that are most healthy, as well as for determination of more specific traits, such as sex selection in which spermatozoa are separated into X- (female) and Y- (male) chromosome bearing populations based on their difference in DNA content. (wikipedia.org)
  • As the X chromosome is larger (i.e. has more DNA) than the Y chromosome, the "female" (X-chromosome bearing) spermatozoa will absorb a greater amount of dye than its male (Y-chromosome bearing) counterpart. (wikipedia.org)
  • Spermatozoa are the mature male gametes in many sexually reproducing organisms. (wikipedia.org)
  • In 2014, Peter Michalik and Martín Ramírez published a study of the morphology of the reproductive system of male spiders, including their spermatozoa. (wikipedia.org)
  • fluid
  • As the spermatozoa pass through the flow cytometer in single file, each spermatozoon is encased by a single droplet of fluid and assigned an electric charge corresponding to its chromosome status (e.g. (wikipedia.org)
  • protein
  • We determined that l -amino acid oxidase, mitochondrial malate dehydrogenase 2, NAD (MDH2), cytosolic 5′-nucleotidase 1B, lysozyme-like protein 4, and calmodulin (CALM) were significantly and abundantly expressed in high-litter size spermatozoa. (pubmedcentralcanada.ca)
  • different
  • 2002. Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: Influence of adding and removing cryoprotectant. (wikipedia.org)
  • female
  • They are united by having simpler genitalia than other araneomorph spiders, lacking a cribellum, and sharing an evolutionary history of synspermia - a particular way in which spermatozoa are grouped together when transferred to the female. (wikipedia.org)
  • techniques
  • This article describes two popular sperm freezing techniques employed by mouse repositories to archive spermatozoa using cryoprotective agents supplemented with either L ‐glutamine or monothioglycerol. (currentprotocols.com)