• This study was conducted to investigate the relationship of semen parameters in samples used for intracytoplasmic sperm injection (ICSI) with fertilization and pregnancy rates in infertile couples. (reproscientifics.com)
  • Intracytoplasmic sperm injection ( ICSI / ˈ ɪ k s i / IK -see ) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg . (wikipedia.org)
  • Schematic image of intracytoplasmic sperm injection in the context of IVF . (wikipedia.org)
  • The most effective treatment is usually in vitro fertilization via intracytoplasmic sperm injection. (msdmanuals.com)
  • After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
  • Sperm Tracer is a unique AI technological software that supports fertility experts and embryologists to evaluate, in real-time, the best sperm for ICSI procedures. (reproscientifics.com)
  • The sperm quality outcome measures were motility, viability and concentration, which are the parameters most frequently used in clinical settings to assess fertility. (qigonginstitute.org)
  • There is a growing body of science showing serious impacts to sperm and fertility from cell phone radiation. (electromagnetichealth.org)
  • For example, in a study published in Fertility and Sterility (Vol. 89) in 2008 by A. Agarwal et al of the Cleveland Clinic, laboratory values of sperm count at an infertility clinic decreased as the duration of daily exposure to cell phones increased. (electromagnetichealth.org)
  • Since the bead concentration is known, a simple calculation determines the sperm concentration, an important parameter of fertility potential. (purdue.edu)
  • This includes, 1) how small RNAs regulate gene expression in the male germline to support spermatogenesis and fertility, 2) how small RNAs are packaged into mature sperm, 3) how RNAs transmitted during fertilization are able to regulate early embryonic gene expression and development, and 4) how this regulation can alter developmental programs to produce a non-genetically inherited phenotype. (upenn.edu)
  • According to new research, cellphone exposure may not only affect male fertility, but even sperm viability. (newsone.com)
  • While testosterone is necessary for a wide variety of things, including sperm production, bone growth, and muscle development, its primary purpose is to help maintain male fertility. (erec-renewables.org)
  • Male rats were given 0, 750, 1500 or 3000 mg/kg neat JP-8 daily by gavage for 70 days prior to mating with naive females to assess fertility and sperm parameters. (europa.eu)
  • 40 :g/dL also may be associated with adverse effects on sperm counts and morphology (ATSDR 1999). (cdc.gov)
  • The sperm parameters including sperm motion, morphology, viability and survivability were recorded in males. (europa.eu)
  • The percentage of sperms with abnormal morphology significantly increased in 50, 200 and 800 mg/kg groups in comparison to control. (europa.eu)
  • Documented cell phone impacts on sperm include: decreased sperm count, motility, viability, and normal morphology. (electromagnetichealth.org)
  • It can be used in teratozoospermia , because once the egg is fertilized, abnormal sperm morphology does not appear to influence blastocyst development or blastocyst morphology. (wikipedia.org)
  • [6] Even with severe teratozoospermia, microscopy can still detect the few sperm cells that have a "normal" morphology, allowing for optimal success rate. (wikipedia.org)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • Concentration of 4 mg / ml in the control treatment vitamin C was 81% and 85% viability, with E motility was 80% and 85% viability. (journalcra.com)
  • Exposure to radiofrequency radiation is associated in human studies with lower sperm concentration, viability, and motility. (ewg.org)
  • and sperm viability and acrosome integrity at ≥ 100 µg/mL GLY-equivalent concentration as early as 1 h of incubation. (nih.gov)
  • Exposure to mobile phones was associated with reduced sperm motility (mean difference -8.1% (95% CI -13.1, -3.2)) and viability (mean difference -9.1% (95% CI -18.4, 0.2)), but the effects on concentration were more equivocal. (qigonginstitute.org)
  • We conclude that pooled results from in vitro and in vivo studies suggest that mobile phone exposure negatively affects sperm quality. (qigonginstitute.org)
  • This study strongly suggests that being exposed to radio-frequency electromagnetic radiation from carrying mobiles in trouser pockets negatively affects sperm quality. (newsone.com)
  • Effect of MBCD on viability of frozen-thawed bovine sperm. (biomedcentral.com)
  • The aim of this study was to assess the effect on the use of vitamin C, E and C + E as antioxidants on sperm quality of frozen-thawed semen boar semen Yorkshire breed, which was divided into three treatments with Vitamin C, E and combined (C + E) at different concentrations (0, 2, 4 and 6 mg / ml) was used. (journalcra.com)
  • A small dose of E 2 (2 µg/mL) had a beneficial effect on the motility and acrosome integrity of bull sperm in vitro . (scielo.org.za)
  • 0.05) decreased sperm motility, viability, mitochondrial activity and acrosome integrity but had no detrimental effect at lower doses. (nih.gov)
  • In terms of insemination, ICSI needs only one sperm cell per oocyte , while IVF needs 50,000-100,000. (wikipedia.org)
  • This condition is called non-obstructive or secretory azoospermia, as opposed to obstructive azoospermia, in which complete sperm production does occur in the testicles, and potentially fertilizing spermatozoa can be obtained by testicular sperm extraction (TESE) and used for ICSI. (wikipedia.org)
  • Moreover, the distinction between living round spermatids, to be used in ROSI, and dead round spermatids, to be discarded, needs specific methods and skills, not required in the case of ICSI where sperm cell viability can be easily evaluated on the basis of sperm motility in most cases. (wikipedia.org)
  • Oxidative stress has numerous effects, including increased apoptosis, reduced motion parameters, and reduced sperm integrity. (mdpi.com)
  • In a similar fashion, GLY and Roundup did not inflict any detrimental effect on sperm DNA integrity. (nih.gov)
  • CIFTCI, H.B. and ZULKADIR, U. . The effect of oestradiol-17 β on the motility, viability and the acrosomal status of bull sperm . (scielo.org.za)
  • The aim of this study was to test the effect of oestradiol-17β (E 2 ) on the motility, viability and the acrosomal status of bull sperm in vitro . (scielo.org.za)
  • Note the higher proportion of sperm with increased levels of red fluorescence in cytogram B compared to A and the resulting shift in the alpha t distributions (compare C to D). (purdue.edu)
  • Similar effects were seen for sperm viability, which refers to the proportion of sperm that were alive, according to the study published June 9 in the journal Environment International . (newsone.com)
  • Sperm cells are particularly susceptible to damage from free radicals that can be generated from radiofrequency radiation exposure, since they lack the protective antioxidants typical of other cells in the body. (ewg.org)
  • Evidence of the effects of radiofrequency radiation exposure on sperm quality has also been observed in animal studies. (ewg.org)
  • A systematic review was therefore conducted, followed by meta-analysis using random effects models, to determine whether exposure to RF-EMR emitted from mobile phones affects human sperm quality. (qigonginstitute.org)
  • In the highest exposure group, 1/3 of sperm count was lost with over 4 hours of male cell phone use, and 50% of the remaining sperm showed physical abnormalities, did not swim well or were non-viable. (electromagnetichealth.org)
  • In the study, when men had no exposure to cellphones, 50 percent to 85 percent of their sperm had a normal ability to move towards an egg. (newsone.com)
  • Consequently, such men can become estrogen dominant, leading to weight gain and decreased sperm production. (depopulation.news)
  • The R123/Pl assay provides a quantitative measurement of mitochondrial membrane potential (cell motility) and cell membrane viability. (purdue.edu)
  • After staining with R123, fluorescence intensity in the sperm midpiece is related to the mitochondrial membrane potential and motility. (purdue.edu)
  • Higher dose of E 2 (8 µg/mL) had a detrimental effect on viability. (scielo.org.za)
  • In normal cell biology, ROS levels increase with a decreasing antioxidant response, resulting in oxidative stress which threatens sperm biology. (mdpi.com)
  • As boar sperm are particularly sensitive to oxidative stress, this study evaluated the role of SP antioxidants in the observed differences in sperm cryotolerance among ejaculate fractions. (diva-portal.org)
  • These results demonstrated that certain SP antioxidants are positively involved in boar sperm cryotolerance, minimizing the oxidative stress imposed by cryogenic handling. (diva-portal.org)
  • LD50 dose and melanization capabilities), body growth, and reproductive investment (sperm viability and ovarian mass) measured. (creighton.edu)
  • The reproductive NOAEL (No Observed Adverse Effect Level) for male SD rats was 12,5 mg/kg/day based on findings of necropsy, organ weight measurement, sperm test findings and histopathology. (europa.eu)
  • Each embryo carries 50% of the genetic information of an oocyte and 50% of a sperm. (reproscientifics.com)
  • Every single sperm participating with 50% of the DNA cargo of the embryo, could play a detrimental role for the embryo itself and its quality. (reproscientifics.com)
  • Figure 3: Two parameter isometric displays of green and red fluorescence from 5000 bull sperm stained with Rhodamine 123 and Propidium Iodide (RI23/Pl). Sperm samples from known fertile (A) and less fertile (B) donors are shown. (purdue.edu)
  • Figure 4: Two parameter green (log) versus red (linear) fluorescence isometric displays of 10,000 cells collected from fertile (A) and less fertile (B) bull sperm stained with SYBRI4 and counterstained with Propidium Iodide (PI). (purdue.edu)
  • In cases of nonobstructive (secretory) azoospermia, on the other hand, testicular sperm production is blocked at different stages of the process of sperm formation (spermatogenesis). (wikipedia.org)
  • However, in the 200 and 800 mg/kg groups the relative and absolute weights of testis and epididymis were significantly decreased.The results of sperm analysesshowedsignificantlyreduced ofsperm motility, viability, survivability and the number of sperms in the left epididymis in the group treated with 50 mg/kg or more of the test chemical compared with controls. (europa.eu)
  • The epididymal head overhangs the upper pole of the testis, receives the seminal fluid from the ducts of the testis (which pierce the upper portion of the mediastinum), then allows the passage of the sperm into the distal portion of the epididymis. (medscape.com)
  • Due to its length, the epididymal duct allows space for storage and maturation of sperm. (medscape.com)
  • During the incubation, the number of motile, viable sperm and the number of sperm possessing lost/damaged acrosomes, cytoplasmic droplets and coiled tails were counted at 0, 4, 18 and 24 h of incubation. (scielo.org.za)
  • Lab technicians will process the sperm sample using specialized techniques to collect the most motile (active) sperm. (sutterhealth.org)
  • In animal studies, sperm "heads" show more abnormalities after radiofrequency radiation, compared to sperm heads in unexposed animals. (ewg.org)
  • It also affects viability of sperm and dermatitis. (wikipedia.org)
  • Once bound to those cells, it interferes with the sperms' ability to swim - essential to its role in fertilizing an egg. (askmen.com)
  • Once the ATP molecules are broken down, the sperm lose their ability to swim. (erec-renewables.org)
  • Lower doses of E 2 (2 and 4 µg/mL) did not affect viability of sperm, but a high dose of E 2 (8 µg/mL) caused reductions in viability at 4 and 24 h of incubation. (scielo.org.za)
  • The conception process takes place when a sperm cell from the male unites with an ovum (egg) in the female's fallopian tube in a process called fertilization. (bartleby.com)
  • The sperm cells of cell phone users were abnormally formed, compared to people who don't use cell phones. (ewg.org)
  • Sperm motility is affected by proteins called myosins, which are found in the tail of each sperm cell. (erec-renewables.org)
  • cell focus measurement and cell viability counts. (c-imt.org)
  • The ongoing chemical assault on the male species is apparently working as planned, with new evidence showing that sperm counts in Western men have declined to record lows. (depopulation.news)
  • However, their research, published in the journal Human Reproduction Update , suggests that sperm counts are declining all over the world, including in still-developing countries. (depopulation.news)
  • The study supports earlier research suggesting that sperm counts have been on a progressive decline for a number of years. (depopulation.news)
  • Constant worry, anxiety, and busyness is likely another major cause of decreasing sperm counts. (depopulation.news)
  • sperm counts. (c-imt.org)
  • Male factor infertility (low sperm count or low motility). (sutterhealth.org)
  • This procedure is most commonly used to overcome male infertility problems, although it may also be used where eggs cannot easily be penetrated by sperm, and occasionally in addition to sperm donation . (wikipedia.org)
  • Another rare mechanism of infertility is destruction or inactivation of sperm by sperm antibodies, which are usually produced by the man. (msdmanuals.com)
  • The andromerogones for 5 sperm species showed a half of their respective diploid chromosome numbers without chromosome elimination. (bioone.org)
  • This method is applicable for analysis of the haploid male chromosome complement in sea urchin species for which only sperm can be obtained. (bioone.org)
  • The males of the species produce two "packets" of sperm that are stuck to the female during intercourse. (sott.net)
  • The number of sperm cells possessing proximal and distal cytoplasmic droplets and the number of sperm cells bearing coiled tails were not altered by any of the treatments. (scielo.org.za)
  • The percentage of FT sperm exhibiting fragmented nuclear DNA did not differ among ejaculate fractions and the EE. (diva-portal.org)
  • An RNAi construct selectively driven in cyst cells leads to male sterility, demonstrating the necessity of noa function for male germline development and the interaction of the somatic cyst cells with the developing sperm. (biologists.com)
  • We developed a method for preparing male chromosomes from sea urchin hybrid andromerogones created with cryopreserved sperm. (bioone.org)
  • As a result, the sperm in effect have a "use-by" viability date, after which the male becomes impotent. (sott.net)
  • BGU researchers in Beersheba headed by Prof. Amir Sagi of the marine biology and biotechnology study center discovered that the male whiteleg have a mechanism that - during their youth - allows them to avoid the stiffening of the sperm packets and resulting impotence. (sott.net)
  • This means that male whitelegs renew their sperm packets in a cyclical manner, once every two weeks, in perfect coordination with losing their armor, a process that is accomplished through hormonal supervision. (sott.net)
  • AI semen must be kept at 17C and boars kept cool to maintain sperm viability. (thepigsite.com)
  • Ten ejaculates from five boars were manually collected in fractions: the first 10 mL of the sperm-rich fraction (SRF), the rest of the SRF and the post-SRF. (diva-portal.org)
  • Boar ejaculates are ejected in fractions with a specific composition in terms of sperm numbers and seminal plasma (SP), which is reflected in the varying sperm cryotolerance observed among different fractions. (diva-portal.org)
  • Effect of mobile telephones on sperm quality: A systematic review and meta-analysis. (qigonginstitute.org)
  • Sperm disorders include defects in quality or quantity of sperm produced and defects in sperm emission. (msdmanuals.com)
  • Just a 50% (or less) reduction in sperm mobility can render a man effectively infertile, according to the World Health Organization. (askmen.com)
  • When couples are infertile, the man should always be evaluated for sperm disorders. (msdmanuals.com)
  • The number of forward progressing sperm was increased by the supplementation of 2 and 8 µg E 2 /mL over the control group at the 4 h incubation. (scielo.org.za)
  • The number of sperm cells with lost acrosomes was significantly high in control group at 24 h of incubation. (scielo.org.za)
  • This is an urgent wake-up call for researchers and health authorities around the world to investigate the causes of the sharp drop in sperm count , with the goal of prevention," stated Dr. Hagai Levine, one of the lead authors of the study. (depopulation.news)
  • This study sought to investigate, using the pig as a model, the impact of pure glyphosate and its most known commercial formulation, Roundup, on sperm function and survival. (nih.gov)
  • Radio-frequency electromagnetic radiation (RF-EMR) from these devices could potentially affect sperm development and function. (qigonginstitute.org)
  • DNA strand breaks in fertilizing sperm nuclei have potentially serious consequences for developing embryos. (purdue.edu)
  • Males that have not mated were found to have lost their old sperm packets near the last few hours of their "periods," and new ones appear in their place. (sott.net)
  • Figure 2: The SCSA can be done concomitantly with a sperm count determination by admixing a known number of fluorescent beads with the sperm sample (13). (purdue.edu)
  • However, data indicate that daily ejaculation does not reduce the sperm count in men unless there is a problem. (msdmanuals.com)
  • If successful, it would hinder sperm mobility without the side effects associated with hormonal treatments. (askmen.com)
  • After staining with SYBR-14 and PI, the viability of sperm was analyzed by flow cytometry method (fluorescent intensity of SYBR-14 and PI, top ). (biomedcentral.com)
  • In the lab, we used the techniques of medicinal chemistry to create a derivative of ouabain that is good at zeroing in on the α4 transporter in sperm cells in rats. (askmen.com)
  • Co-staining sperm with SYBRI4 and PI readily identifies cells with intact membranes versus membrane compromised cells. (purdue.edu)
  • This is because the acrosome reaction has to take place and thousands of sperm cells have to be involved in IVF. (wikipedia.org)
  • Retrieved eggs undergo examination by a gamete biologist for maturity and viability. (sutterhealth.org)