Sperm in vitro oocyte maturation techniques. Medical search. Frequent questions

Instead, many efforts concentrated on conventional in vitro fertilization (IVF). (ivis.org)
11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
Her clinical and research interests are related to assisted reproductive technologies in horses, with an emphasis on transvaginal oocyte aspiration, in vitro oocyte maturation, fertilization, and embryo culture. (tamu.edu)
This discovery may soon allow them to avoid using abnormal - or aneuploid - eggs during infertility treatments, and instead to pick eggs that are healthy enough for a successful in vitro fertilization (IVF) cycle. (health.am)
Assisted reproductive technologies (ARTs) are techniques to help a woman become pregnant, including in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and other similar procedures. (health.am)
More than 4 million babies have been born worldwide as a result of using the in vitro fertilization technique. (health.am)
With IVF, a method of assisted reproduction, a man's sperm and the woman's egg are combined in a laboratory dish, where fertilization occurs. (health.am)
Preimplantation genetic testing (PGT) is a technique used to identify chromosomal genetic abnormalities in embryos created through in vitro fertilization (IVF) before pregnancy. (medscape.com)
Advancements in embryo culture, blastocyst biopsy techniques, 24-chromosome aneuploidy screening platforms, and improved genomic coverage of new sequencing platforms, such as next-generation sequencing, have made PGT safe and accessible for all patients who undergo in vitro fertilization. (medscape.com)
The use of preimplantation genetic testing for aneuploidy (PGT-A), formerly known as preimplantation genetic screening or PGS, has increased in recent years, now encompassing an estimated 40% of in vitro fertilization (IVF) cycles in the United States. (medscape.com)
The subsequent steps are identical to those in IVF/ICSI, including oocyte culture, fertilization check, embryo culture and grading, and embryo transfer. (artfertilityclinics.com)
This study was carried out in a private IVF centre in conjunction with Assisted Reproductive Techniques Center of Ain Shams University Maternity Hospital after the approval of the Research Ethics Committee, during the period between Jan 2014 to Jan 2016 and included 60 women, with unexplained infertility undergoing in vitro fertilization (IVF), were randomised into two groups. (peertechzpublications.com)
Despite considerable technological advancements, polyspermy remains a significant challenge in in-vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. (e-jarb.org)
Collectively, these results demonstrate that various fertilization conditions, including modified co-culture period, active sperm separation, and culture medium volume, enhance fertilization efficiency and subsequent embryonic development by decreasing polyspermy occurrence. (e-jarb.org)
Intracytoplasmic sperm injection ( ICSI / ˈ ɪ k s i / IK -see ) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg . (wikipedia.org)
Round spermatid injection (ROSI) is a technique of assisted reproduction whereby a round spermatid is injected into oocyte cytoplasm in order to achieve fertilization. (wikipedia.org)
These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
In vitro Fertilization of retrieved oocytes with recipient's husband's sperm /Donor sperm obtained from Sperm bank. (lushfertility.in)
In vitro fertilization (IVF) involves combining an egg and sperm within a laboratory setting to create an embryo. (ivf.net)
IVF.net is a worldwide resource dedicated to scientists working in the field of In Vitro Fertilization (IVF). (ivf.net)
On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)
However, these techniques have to be carried out very carefully as they, if applied wrongly, bear risks of generating ROS damaging the germ cells and preventing fertilization. (hindawi.com)
Besides affecting fertilization and the outcome of pregnancy, sperm DNA damage has a negative impact on the health of the offspring as uncorrected sperm DNA damage following zygote formation has the potential to create mutations/epimutations in the offspring [ 7 ]. (hindawi.com)
This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)
Furthermore, sperm DNA damage appears to be linked to the most important checkpoints of fertility such as reduced fertilization rates, lower embryo quality and pregnancy rates, higher miscarriage rates, malformations, and childhood diseases [ 22 ]. (hindawi.com)
The oocytes were fertilized by spermatozoa that had been capacitated with two groups of fertilization media, the first group containing melatonin and the second group containing cysteamine. (vetmedmosul.com)
Compared with other groups, sperms treated with melatonin demonstrated hyperactivity, and the fertilization rate was significantly increased. (vetmedmosul.com)
1- Melatonin enhances sperm fertilization ability. (vetmedmosul.com)
In the last decade, in vitro fertilization and sperm capacitation techniques have significantly advanced. (vetmedmosul.com)
ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)
During sperm capacitation, melatonin may have a chemotactic effect that helps guide sperm to fertilization. (vetmedmosul.com)
Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
Sunshine IVF Hospital is one of the most renowned In Vitro Fertilization (IVF) Centres in Amanora,pune region, focusing on Fertility, IVF, Egg & Sperm donation. (drkanade.in)
Throughout this thesis, I analyzed the methylation patterns of germ cells and embryos to determine whether in vitro maturation and in vitro fertilization have a negative impact on the epigenetic patterns. (uni-wuerzburg.de)
In a total of 141 sperm samples from couples undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) including 106 with male factor or combined infertility and 28 with female infertility, I detected a significant correlation between lower quality of semen parameters (sperm count, percentage of abnormal sperm, and percentage of motile sperm) and the rate of imprinting errors. (uni-wuerzburg.de)
Comparison of embryos after natural conception, in vitro fertilized embryos from superovulated oocytes, and embryos achieved through fertilization of in vitro cultured oocytes revealed no dramatic effect on the imprinting patterns of Igf2r, H19, and Snrpn. (uni-wuerzburg.de)
As a matter of fact, Ahmedabad ranks in the top position in IVF (In vitro fertilization) treatment among the cities, Surat, Bhav Nagar, Vadodara, Gandhi Nagar, Rajkot in Gujarat, and here Wellspring IVF Hospital is India's most reputed and Famous in vitro fertilization Centre. (wellspringivfsurrogacy.com)
Being the best place for IVF In vitro fertilization treatment in India, our fertility specialists bring happiness to people's life by practicing complete transparency in the procedure. (wellspringivfsurrogacy.com)
There has been a dramatic rise in the use of Assisted Reproductive Technologies (ART), including In Vitro Fertilization (IVF) throughout the world over the past three decades. (virascience.com)
He is chair of the Quality Assurance Committee for the Society of Assisted Reproductive Technologies, the professional organization that oversees the delivery of in vitro fertilization care throughout the United States. (shadygrovefertility.com)
The aim of the present study was to evaluate the association between follicular fluid (FF) levels of high‑mobility group box 1 (HMGB1) protein and the reproductive outcome in patients undergoing in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). (spandidos-publications.com)
Lédée N, Frydman R, Osipova A, et al: Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles. (spandidos-publications.com)
Hasegawa J, Iwasaki S, Yanaihara A, Negishi M, Tahara R and Okai T: Correlations between steroids concentration in follicular fluid, pronuclear morphology and embryo qualities in vitro fertilization. (spandidos-publications.com)

Retrospectively, it seems absurd, but the respected big-bearded academics disregarded the obvious fact that - unlike those of frogs and salamanders - oocytes and embryos of mammals are extremely sensitive to temperature changes and do not tolerate exposition to room temperature, not even for a short period. (vitavitro.com)
This tissue can later be thawed and sperm extracted for usage with eggs to create embryos. (oasisindia.in)
Oocytes, sperms, embryos and testicular tissue can be cryopreserved and can later be used for conception. (oasisindia.in)
In this technique women's eggs are fertilized with the sperm of the husband and then frozen, so that the embryos can be used when the couple is ready to have a baby. (oasisindia.in)
Embryos produced outside the breeding season can be frozen for later transfer, which makes these techniques very versatile. (ivis.org)
However, other procedures based on the in vivo and in vitro production of equine embryos have emerged in recent years. (ivis.org)
Reasons for the delayed development of in vivo and in vitro production of equine embryos include the scarce availability of abattoir ovaries and the lack of interest from horse breeders and registries. (ivis.org)
In 2021, she began her doctoral studies, under Dr. Charles Love, focused on the study of various clinical factors associated with the efficiency of assisted reproductive technologies in the mare, particularly Intracytoplasmic Sperm Injection (ICSI) and in vitro culture of equine embryos. (tamu.edu)
Various of these studies are conducted in collaboration with Dr. Terje Raudsepp, including the validation of alternative methods of preimplantation genetic diagnosis in embryos, and the impact of ICSI and in vitro culture on the genome and transcriptome of equine embryos. (tamu.edu)
She also has interests in the application of molecular biology techniques for gametes and embryos, and the relationship of these to clinical equine reproduction. (tamu.edu)
Successful implantation requires good quality of embryos, receptive endometrium, and proper embryo transfer technique" [1]. (peertechzpublications.com)
Along with polyspermy, the suboptimal quality of IVF-derived pig embryos poses another challenge that needs to be addressed for in vitro production systems. (e-jarb.org)
Assisted reproductive techniques (ARTs) involve manipulation of sperm and ova or embryos in vitro with the goal of producing a pregnancy. (msdmanuals.com)
This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus. (wikipedia.org)
If all requirements for round spermatid selection and injection are successfully met, the injected oocytes develop to early embryos and can be transferred to the mother's uterus to produce pregnancy. (wikipedia.org)
Paternal sperm DNA damage has negative effects on the integrity of early embryonic development as the percentage of good quality embryos as well as implantation rates was significantly reduced in patients exhibiting high DNA damage [ 27 ] and it appears that the second and third mitoses are the sensitive periods [ 28 ]. (hindawi.com)
oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation. (vetmedmosul.com)
Oocytes, sperms, and embryos are shielded from oxidative stress in their natural environment by free radical scavengers found in oviductal and follicular fluids. (vetmedmosul.com)
Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
Students will freeze, thaw and analyse sperm samples, oocytes and embryos Students will gain a better understanding of media components by making and designing their own media. (monash.edu)
I did not observe a detrimental effect on the methylation patterns of oocytes and the resulting embryos using in vitro maturation of oocytes and/or standard IVF with in vivo grown superovulated oocytes. (uni-wuerzburg.de)
After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)

ICSI produced the first pregnancy derived from an in vitro matured oocyte [ 5 ], and it was successfully carried to term. (ivis.org)
The next step is to evaluate the maturity and inject the mature ones with sperm from the husband (ICSI). (artfertilityclinics.com)
Intracytoplasmic sperm injection (ICSI) is a technique developed to overcome male infertility attributed to impaired semen quality. (artfertilityclinics.com)
In terms of insemination, ICSI needs only one sperm cell per oocyte , while IVF needs 50,000-100,000. (wikipedia.org)
This condition is called non-obstructive or secretory azoospermia, as opposed to obstructive azoospermia, in which complete sperm production does occur in the testicles, and potentially fertilizing spermatozoa can be obtained by testicular sperm extraction (TESE) and used for ICSI. (wikipedia.org)
Even though many technical aspects of ROSI are similar to those of ICSI, there are also significant differences between both techniques. (wikipedia.org)
Moreover, the distinction between living round spermatids, to be used in ROSI, and dead round spermatids, to be discarded, needs specific methods and skills, not required in the case of ICSI where sperm cell viability can be easily evaluated on the basis of sperm motility in most cases. (wikipedia.org)
[2] The microinjection procedure for ROSI also differs slightly from that of ICSI, since additional stimuli are needed to ensure proper oocyte activation after spermatid injection. (wikipedia.org)
The level of sperm DNA fragmentation may give a sense of guidance as to the appropriate method of ARTs to employ especially between IVF and ICSI [ 17 , 18 ]. (hindawi.com)

At this point, intracytoplasmic sperm injection-injection of a single sperm into each oocyte-may be done, particularly if spermatogenesis is abnormal in the male partner. (msdmanuals.com)
Schematic image of intracytoplasmic sperm injection in the context of IVF . (wikipedia.org)
This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)

In each process, a robust number of mouse oocytes can be generated in a dish. (researchsquare.com)

Through its growth and maturation, it reaches extreme levels of specialization, while maintaining at the same time a condition of totipotency. (ivf.net)
From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development. (ivf.net)
Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
Driancourt MA and Thuel B: Control of oocyte growth and maturation by follicular cells and molecules present in follicular fluid. (spandidos-publications.com)

In G-CSF group (n=30), 300 µg trans-cervical intrauterine of G-CSF was administered at the oocyte retrieval day. (peertechzpublications.com)
pregnancy rates with this technique are lower than those with retrieval of multiple oocytes, but costs are lower and success rates are increasing. (msdmanuals.com)
Oocyte retrieval from donor. (lushfertility.in)
Donor egg program includes oocyte retrieval from healthy young oocyte. (lushfertility.in)
Once ovarian follicles grow to an appropriate size, a trigger is administered to mature the oocytes in preparation for oocyte retrieval. (plandl.best)
Smith MP, Flannery GR, Randle BJ, Jenkins JM and Holmes CH: Leukocyte origin and profile in follicular aspirates at oocyte retrieval. (spandidos-publications.com)

Today, the most practical use of OPU or transabdominal follicle aspiration is to recover in vivo matured oocytes [ 9 ] for oocyte transfer. (ivis.org)
In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
2004). In vivo and in vitro differentiation of male germ cells in the mouse. (edu.au)
This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
We have recently established a novel culture system in which mouse pluripotent stem cells differentiate to mature oocytes in a manner similar to oogenesis in vivo. (researchsquare.com)

Immature oocytes are harvested and cultivated for 24-48 hours in specific media enriched with hormones. (artfertilityclinics.com)
Epididymal spermatozoas are often described as immature, immotile, and infertile sperm cells, yet the epididymis is the organ where spermatozoa mature, acquire motility, and become fully fertile. (encyclopedia.pub)
Background: Induction of in vitro maturation and development of immature oocyte is one of the methods that has been frequently used recently in artificial reproduction techniques and has drawn attention of many investigators. (virascience.com)

About 34 hours after hCG is given, oocytes are retrieved by direct needle puncture of the follicle, usually transvaginally with ultrasound guidance or less commonly laparoscopically. (msdmanuals.com)
To identify follicle sizes on the day of trigger most likely to yield a mature oocyte following hCG, GnRH agonist (GnRHa), or kisspeptin during IVF treatment. (plandl.best)
Using simulated follicle size profiles of patients with 20 follicles on the day of trigger, our model predicts that the number of oocytes retrieved would increase from a mean 9.8 (95% prediction limit 9.3-10.3) to 14.8 (95% prediction limit 13.3-16.3) oocytes due to the difference in follicle size profile alone. (plandl.best)
However, limited data exist to establish which follicle size on the day of trigger is most likely to yield a mature oocyte. (plandl.best)
Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
Removal of the oocyte from the follicle causes meiosis to progress in the oocyte. (wikipedia.org)
The oocytes compete with one another to become the dominant follicle and eventually ovulate to be released as an egg capable of being fertilized. (medscape.com)

This protocol consists of a culture method that can be mainly divided into four periods: primordial germ cell-like cell (PGCLC) derivation, primary oocyte differentiation (in vitro differentiation: IVDi), oocyte growth to germinal vesicle (GV) oocytes (in vitro growth: IVG) and oocyte maturation (in vitro maturation: IVM). (researchsquare.com)

Decline in the Number of Oocytes from Birth to Menopause. (ldysinger.com)
The decline in the number of oocytes begins at 20 weeks' gestation when the female fetus has approximately 6-7 million oogonia (largest lifetime endowment). (medscape.com)

We offer Oocyte, Sperm and Embryo Vitrification along with Testicular Tissue Cryopreservation and In Vitro Maturation of Oocytes as part of the fertility preservation program. (oasisindia.in)
Semen cryopreservation is the oldest and the most successful option for fertility preservation in male cancer survivors, where the sperms are frozen before initiating cancer treatments like chemotherapy or radiotherapy. (oasisindia.in)
In this video, we describe a protocol for the vitrification of sheep oocyte, collected from both juvenile and adult donors, and in vitro matured to prior to cryopreservation. (jove.com)
The theoretical aspects of IVF, embryo production and cryopreservation will be covered in the previous unit reinforced by extensive practical training The theoretical knowledge gained in slow cooling and vitrification techniques will also enhanced by practical training. (monash.edu)

[2] It is developed from the primary oocyte by maturation. (wikipedia.org)

Mahadevan MM and Fleetham J: Relationship of a human oocyte scoring system to oocyte maturity and fertilizing capacity. (spandidos-publications.com)

In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep. (vetmedmosul.com)

Oocytes are surrounded by cells, called cumulus cells, which regulate and assist the process of egg maturation. (health.am)
The identification of these genes in cumulus cells can serve as a novel, non-invasive marker to identify abnormal oocytes and thus ultimately improve IVF success rates," said Patrizio, professor in the Department of Obstetrics, Gynecology & Reproductive Sciences at Yale. (health.am)
After recovering the Cumulus oocytes complex, where matured in vitro for 24 hours in static a condition that include for juvenile oocyte, the supplementation with hundred micromolar of trehalose in maturation medium. (jove.com)
Following in vitro maturation, the oocytes were denuded of Cumulus cells mechanically by gently pipetting and examined under stereo microscope, with 60x magnification. (jove.com)

In other words, a sperm cell found in the caput of the epididymis possesses characteristics that are different from sperm cells found in the corpus or cauda of the same epididymis, such as the cytoplasmic droplet position. (encyclopedia.pub)
In the initial segment of the epididymis, the sperm cell is immotile-unable to fertilize an oocyte and has a proximal cytoplasmic droplet-close to the neck [ 3 ] . (encyclopedia.pub)

Retrospective analysis to determine the size of follicles on day of trigger contributing most to the number of mature oocytes retrieved using generalized linear regression and random forest models applied to data from IVF cycles (2014-2017) in which either hCG, GnRHa, or kisspeptin trigger was used. (plandl.best)
Follicles 12-19 mm on the day of trigger contributed the most to the number of oocytes and mature oocytes retrieved. (plandl.best)

After sufficient follicular growth, human chorionic gonadotropin (hCG) is given to trigger final follicular maturation and ovulation. (msdmanuals.com)
Belayet HM, Kanayama N, Khatun S, Asahina T, Okada Y, Kitamura K, Kobayashi T and Terao T: Pharmacologic doses of interleukin 8 suppositories induce follicular maturation in rabbits. (spandidos-publications.com)

This procedure is most commonly used to overcome male infertility problems, although it may also be used where eggs cannot easily be penetrated by sperm, and occasionally in addition to sperm donation . (wikipedia.org)

As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)

It can be used in teratozoospermia , because once the egg is fertilized, abnormal sperm morphology does not appear to influence blastocyst development or blastocyst morphology. (wikipedia.org)
[6] Even with severe teratozoospermia, microscopy can still detect the few sperm cells that have a "normal" morphology, allowing for optimal success rate. (wikipedia.org)
As a result, numerous authors have suggested an introduction of sperm DNA damage testing into the andrological laboratory workup as an independent tool as DNA fragmentation apart from normal sperm morphology appears to be a reliable and more robust parameter than conventional semen analysis due to its low biological variability and thus is a useful biomarker that should be implemented in any andrological diagnostic workup [ 19 - 21 ]. (hindawi.com)

Mammalian ovaries are endowed with a huge number of small oocytes (primordial oocytes) in primordial follicles. (go.jp)
A small number of primordial oocytes start to grow, while others remain quiescent. (go.jp)
Little is known about the mechanism regulating the activation of primordial oocytes. (go.jp)
We think that primordial oocytes in adult mammals are different from those in neonatal mammals. (go.jp)
In this review, we summarize the results regarding the activation of primordial oocytes in neonatal and adult ovaries of different species and propose a model in which ovaries of neonatal mammals contain a mixed population of both quiescent and activated primordial oocytes, while almost all primordial oocytes are quiescent in adult females. (go.jp)
The dormancy of primordial oocytes may be required to reserve the non-growing oocyte pool for the long reproductive life in mammals. (go.jp)
FOXO3 is considered one of the molecules responsible for the dormancy of primordial oocytes in adult ovaries. (go.jp)
These quiescent primordial oocytes are activated, perhaps by certain mechanisms involving the interaction between stimulatory and inhibitory factors, to enter the growth phase. (go.jp)
The PGCLCs were almost identical to primordial germ cells (PGCs), the precursor of eggs and sperm, in terms of gene expression, epigenetic status and functionality. (researchsquare.com)

There, she was trained in transvaginal oocyte aspiration, oocyte and embryo manipulation in the mare, and conducted studies on in vitro capacitation of stallion sperm. (tamu.edu)
TESA /TESE ( Testicular sperm aspiration or extraction) - is a surgical procedure where in. (lushfertility.in)

Oogenesis incorporates a unique sequence of differentiation processes, during which biologically significant events such as meiosis and oocyte growth are orchestrated with communication to surrounding somatic cells. (researchsquare.com)

[ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)
Percoll treatment increased the rate of oocyte penetration and blastocyst formation compared to the control. (e-jarb.org)
The large volume of medium significantly reduced the number of dead oocytes and increased the rate of blastocyst formation compared to the small volume. (e-jarb.org)

It has been hypothesized that in gonadal dysgenesis various deletions on the X chromosome cause such rapid atresia of oocytes 5 that few girls with this condition reach puberty with functioning ovaries. (ldysinger.com)
Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
4 Of note, PGCLCs gave rise to sperm and oocytes by transplantation into neonatal testes and adult ovaries, respectively, 4,5 which means that PGCLCs have the potential to give rise to functional gametes. (researchsquare.com)

For assisted reproductive techniques, oocytes and sperm are collected from the intended parents or donors, and an embryo or the gametes are transferred to the woman's reproductive tract after culture in vitro. (msdmanuals.com)

Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle -coordinating centrosomes . (wikipedia.org)
Prior to the onset of menopause (10-15 y), menstrual cycles shorten and rapid follicular loss occurs because more oocytes are being recruited. (medscape.com)

Ali Hassan, H. Epididymal Sperm of Canine and Feline. (encyclopedia.pub)
Epididymal Sperm of Canine and Feline" Encyclopedia , https://encyclopedia.pub/entry/15218 (accessed November 30, 2023). (encyclopedia.pub)
Moreover, in cats, a species notoriously difficult to collect semen from, epididymal sperm may serve as an extra and easily accessible source of gametes [ 2 ] . (encyclopedia.pub)

The maintaining of oocyte potential viability after longterm storage represent a tool of great opportunity, as it would improve domestic animal breeding by genetic selection programs, contribute to preserve biodiversity through wildlife species conservation, and increase the research in biotechnology. (jove.com)
thereby, oocytes and sperm are highly vulnerable to reactive oxygen species (ROS) attacks, affecting their quality and viability (6). (vetmedmosul.com)

In vitro maturation (IVM) refers to the process when a woman's eggs are collected and matured outside the body. (artfertilityclinics.com)
The number and quality of a woman's oocytes declines with age. (medscape.com)

Only a few oocytes (eggs) per IVF treatment cycle are able to produce a pregnancy because many eggs have the wrong number of chromosomes. (health.am)
Though IVM initially yielded limited success, however, improvements in IVM protocols, maturation procedures, and culture medium have resulted in satisfactory pregnancy rates in a carefully selected patient population. (artfertilityclinics.com)
ALU repeats displayed a higher methylation in sperm DNA of patients leading to a pregnancy and live birth, compared to patients in which pregnancy was not achieved or a spontaneous abortion occurred. (uni-wuerzburg.de)

To resolve this problem, various sperm-selection methods are routinely applied to prepare semen for IVF in various species. (e-jarb.org)
The semen sample is typically washed several times with tissue culture medium and is concentrated for motile sperm, which are then added to the medium containing the oocytes. (msdmanuals.com)

In the fifties of the last century, we had already the nuclear bomb, the sputnik, the television, the first digital computers - but not a single mammalian embryo produced in vitro . (vitavitro.com)
The oocyte is the largest and most mysterious cell in the body of mammalian organisms. (ivf.net)
Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)

Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum . (wikipedia.org)
oocytogenesis , ootidogenesis , and finally maturation to form an ovum (oogenesis proper). (wikipedia.org)

This was a randomized controlled study performed at Assisted Reproductive Techniques Center of Ain Shams University Maternity Hospital, over a 2-year period, between Jan 2014 and Jan 2016, and included 60 infertile women with normal endometrial thickness. (peertechzpublications.com)
Furthermore, DNA methylation was compared between sperm of infertile and presumably fertile controls in order to understand whether epigenetic disturbances lead to infertility at the first place. (uni-wuerzburg.de)

However these treatments are proven to either slow down the oocyte or sperm production or damage the reproductive organs leading to infertility in the patient. (oasisindia.in)
This technique preserves the right tissue and acts as a solution to the infertility issues arising from these treatments. (oasisindia.in)
IVF is also used to overcome infertility caused by endometriosis or problems with the man's sperm (such as low sperm count). (health.am)
IVF can be used to treat infertility due to oligospermia, sperm antibodies, tubal dysfunction, or endometriosis as well as unexplained infertility. (msdmanuals.com)
At the level of human exposure, ATZ reduces sperm count and contribute to infertility in men. (frontiersin.org)

For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E. They were incubated by the standard methods of maturation and capacitation of sperm. (vetmedmosul.com)

In those men in whom spermatogenesis is blocked at the stage of round spermatids, in which meiosis has already been completed, these round cells can successfully fertilize oocytes after being injected into their cytoplasm. (wikipedia.org)
To fertilize the oocytes, sperm must undergo a series of physiological and biochemical alterations in vitro (3). (vetmedmosul.com)

Oogonia enter meiosis during embryonic development, becoming oocytes. (wikipedia.org)

Oxidative stress is one of the key factors contributing to a decrease in sperm quality during the maturation and capacitation of the sperm (5). (vetmedmosul.com)

Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes , a process called oocytogenesis . (wikipedia.org)
Indeed, efforts have been made to reconstitute in vitro oogenesis, 1-3 though none of them succeeded in the production of functional oocytes from pluripotent stem cells. (researchsquare.com)
Here, we established a culture system in which the entire process of oogenesis is reconstituted in a dish, yielding in vitro-generated oocytes that are capable of full-term development to healthy pups. (researchsquare.com)

This technique can be used to enable genetic fatherhood to some men who have no spermatozoa in the ejaculate (azoospermia) and in whom spermatozoa cannot be obtained surgically from the testicles. (wikipedia.org)

Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. (biologists.com)
After the onset of puberty and menses, the female human ovary recruits at least 30-50 oocytes during each menstrual cycle. (medscape.com)

But, with medical advancements, young men and women can opt for fertility preservation techniques where they can vitrify their sperm or oocytes to be used later. (oasisindia.in)
Despite major advancements in assisted reproductive techniques, the implantation rates remain relatively not high. (peertechzpublications.com)
They incorporate all the latest technological advancements taking place in the fertility domain into their treatment techniques. (drkanade.in)

Therefore, in future, melatonin can be implicated as preferable therapeutic especially in IVF and assisted reproductive techniques. (jrhm.org)

in-vitro -matured oocytes were co-incubated with sperm according to a modified two-step culture system. (e-jarb.org)
In experiment 3, fertilized oocytes were cultured in 25 μL microdroplets (10 gametes/drop) or 500 μL (100 gametes/well) of culture medium in 4-well plates. (e-jarb.org)
It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. (jove.com)
The protocol include all the procedures from in vitro maturation, vitrification, warming, and post-warming culture. (jove.com)
This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes. (vetmedmosul.com)
As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)

[5] Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume. (wikipedia.org)
Cycle fecundability decreases as the number of oocytes decreases. (medscape.com)
The number of oocytes decreases to approximately 2-3 million at birth and decreases again to 300,000 by the time of puberty. (medscape.com)

As atresia continues, both the number and quality of oocytes fall below a critical level, and the rate of aneuploidy increases - a finding that is related at least in part to problems of the meiotic spindle 15 , 16 resulting in nondisjunction. (ldysinger.com)

The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)

These findings are linked to melatonin's ability to reduce ROS and its adverse effects on oocytes, such as aging, apoptosis, and its capacity to bind to specific DNA sequences and interact with cytosolic molecules to regulate antioxidant genes (17). (vetmedmosul.com)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)

Anatomy5

Diseases2

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes6

Information Science1

Fertilization43

Embryos23

ICSI9

Intracytoplasmic3

Mouse oocytes1

Growth and maturation4

Retrieval6

Vivo5

Immature3

Follicle7

Germinal vesicle1

Decline in the Number of Oocytes2

Cryopreservation4

Primary oocyte1

Human oocyte1

Competence1

Cumulus4

Cytoplasmic2

Mature oocytes2

Follicular maturation2

Male infertility1

Ovulation1

Morphology3

Primordial9

Aspiration2

Differentiation1

Blastocyst3

Ovaries3

Donors1

Cycles2

Epididymal sperm3

Viability2

Woman's2

Pregnancy3

Semen2

Mammalian3

Ovum2

Infertile2

Infertility5

Treatments1

Fertilize2

Embryonic development1

Capacitation1

Oogenesis3

Spermatozoa1

Ovary2

Advancements3

Melatonin1

Culture6

Decreases3

Aneuploidy1

Embryo production1

Apoptosis1

Involves1