• It was first hypothesized that chordin plays a role in the dorsal homeobox genes in Spemann's organizer. (wikipedia.org)
  • The Self-Organization in Biology: Freiburg Spemann-Mangold Centennial Symposium celebrates 100 years of scientific advances rooted in Hilde Mangold's and Hans Spemann's discovery of the gastrula organizer in vertebrate development, published in 1924 and awarded the Nobel Prize in Physiology or Medicine in 1935. (elsevier.com)
  • Early neurogenesis is initiated by inhibition of BMP signaling in the ectoderm by BMP antagonists expressed in Spemann's organizer region, resulting in anterior neural tissue formation ( Hemmati-Brivanlou and Melton, 1994 ). (molcells.org)
  • In amphibians, the formation of the anterior-posterior (AP) axis is dependent on Spemann's organizer activity( Spemann and Mangold, 1924 ). (silverchair.com)
  • The concept of embryonic induction, first demonstrated in the famous gastrula organizer transplantation experiments by Hilde Mangold and Hans Spemann in their 1924 landmark publication and awarded the Nobel Prize in Physiology or Medicine in 1935, has been the most influential discovery promoting 100 years of research into organization phenomena in biology. (elsevier.com)
  • Spemann and Mangold centennial special issue. (elsevier.com)
  • Gastrulation movements are orchestrated by a small, heterogeneous group of cells with inductive and morphogenetic properties located in the dorsal lip of the blastopore (DBL) of the amphibian gastrula known as the Spemann-Mangold organizer or dorsal organizer. (nature.com)
  • Widely recognized evidence to disprove germ layer theory came in 1922, from Hilde Proescholdt Mangold and her doctoral advisor, Hans Spemann, working at the Zoological Institute in Freiburg, Germany. (asu.edu)
  • Mangold transplanted ectoderm harvested from the dorsal lip, the main organizing tissue of the embryo during gastrulation, between donor and host species of newts. (asu.edu)
  • In a particularly revealing experiment published in 1924, Spemann and his student Hilde Proescholdt Mangold (1898-1924), using salamanders, transplanted a portion of the dorsal lip of the blastopore (the region of the gastrula just above the point of invagination) into the blastocoel, or entral cavity of the blastula of a host embryo. (mbl.edu)
  • Chordin was originally identified in the African clawed frog (Xenopus laevis) in the laboratory of Edward M. De Robertis as a key developmental protein that dorsalizes early vertebrate embryonic tissues. (wikipedia.org)
  • The vertebrate organizer is a specified embryonic tissue that regulates dorsoventral patterning and axis formation. (bvsalud.org)
  • Although numerous cellular signaling pathways have been identified as regulators of the organizer's dynamic functions, the process remains incompletely understood, and as-yet unknown pathways remain to be explored for sophisticated mechanistic understanding of the vertebrate organizer. (bvsalud.org)
  • Investigation of additional candidate genes identified in the cDNA microarray analysis could further delineate the genetic networks of the organizer during vertebrate embryogenesis. (bvsalud.org)
  • To unravel fundamental genetic mechanisms that control cell fate choice in vivo , we study embryonic development in a simple marine invertebrate, the ascidian Ciona intestinalis , belonging to the vertebrate sister group, the tunicates. (uibk.ac.at)
  • Right panels show immunostaining of head organizer cocktail-injected embryos against notochord by MZ15 (dorsal view of the same embryo as shown in the left panel) and somites by 12/101 (lateral view), respectively. (xenbase.org)
  • His techniques of transplanting embryonic regions from younger to older embryos (or vice-versa ) led to the concept of embryonic induction, the process by which previously-differentiated tissues trigger the next stage of differentiation, in a kind of cascade, in the developmental sequence. (mbl.edu)
  • Depletion of xCyp26c by morpholino-oligonucleotides suppressed the normal formation of the axis and head, indicating that xCyp26c plays a critical role in the specification of anterior neural tissue in whole embryos. (molcells.org)
  • Amphibian embryos have provided an excellent model for analysis of embryonic induction because they are easily surgically manipulated and cultured in vitro, and with the addition of treatment with various inducing factors we have been able to control organogenesis and body patterning during early development in vitro. (ehu.eus)
  • Such DNA constructs, when electroporated in fertilised eggs, produce transiently transgenic embryos and allow rapid gene functional and gene regulatory analyses , in precisely identifyable tissue precursors, from earliest embryonic stages in vivo . (uibk.ac.at)
  • TRD13 Mutationsor abnormalities in the hh signaling pathway cause major phenotypic changes;for example, in some hh mutant drosophila the embryo will grow to be short andstubby compared to wild type embryos, and can grow "lawns" of denticles insteadof rows, making the embryo look short and hairy like its namesake (Mohler, 1988).TRD14 Mutationsin hh signaling are typically embryonic lethal, demonstrating the clearimportance of efficient intercellular signaling in development. (wallaceandjames.com)
  • The scope of the conference ranges from embryonic induction and the gastrula organizer itself to its signaling systems, cell behavior during gastrulation, morphogenesis and pattern formation. (elsevier.com)
  • Gastrula fate map (dorsal right) colored by different tissues. (xenbase.org)
  • Stacked bar graphs showing different patterns of enriched spatial expression based on RNA-seq of dissected gastrula tissues (Blitz et al. (xenbase.org)
  • Simultaneously, on the dorsal side of the embryo, axial and paraxial mesoderm tissues undergo convergent extension which elongates the anterior-posterior axis and aids blastopore closure. (nature.com)
  • The Spemann organizer alsoestablishes the anterior/posterior axis in the neural tube via Wnt and BMPsignaling. (wallaceandjames.com)
  • Chordin (from Greek χορδή, string, catgut) is a protein with a prominent role in dorsal-ventral patterning during early embryonic development. (wikipedia.org)
  • TRD7 TheWnt signaling pathway is also crucial for establishing body axes, most notablyin the form of the dorsal/ventral axis by creating the Spemann organizer fromthe Nieuwkoop center, establishing the dorsal side of the embryo (Amerongen andNusse). (wallaceandjames.com)
  • Different pieces of tissue seemed to induce different results, and while the Spemann lab catalogued and correlated those effects, it soon became clear that induction was not a simple linear process. (mbl.edu)
  • Thoma et al observed that Ngn2 alone is sufficient to induce neuronal differentiation in embryonic stem cells. (bartleby.com)
  • The early organizer can induce a complete secondary body axis including head, whereas the late organizer can only induce trunk-tail structures( Spemann, 1931 ). (silverchair.com)
  • Hans Spemann, who directed the Zoological Institute at Freiburg that had been established by August Weismann (1834-1914), accepted Hamburger as a graduate student for the year 1920-21. (mbl.edu)
  • Classic transplantation experiments demonstrated that the inductive properties of the organizer change in the course of development. (silverchair.com)
  • Experiments with zebrafish showed that a chordin gene mutation can lead to less neural and dorsal tissue. (wikipedia.org)
  • Throughout this paper I will detail the early career of Dr. Nusslein-Volhard and the ways in which her research helped to revolutionize the field of developmental biology as it pertains to embryonic development and gene analysis/function. (wepapers.com)
  • We have used conditional gene deletion of mouse β-catenin in Sox17-positive embryonic and extra-embryonic endoderm as well as vascular endothelial progenitors to address the function of canonical Wnt signalling in cell lineage formation and patterning. (biologists.com)
  • Gene Ontology analysis showed that genes involved in embryonic development and growth are significantly over-represented in regenerating early hindlimb buds and that successful regeneration in the Xenopus hindlimb correlates with the induction of stress response pathways. (biomedcentral.com)
  • Gastrulation is a key event in animal embryogenesis during which germ layer precursors are rearranged and the embryonic axes are established. (nature.com)
  • Here we employ ChIP-seq and RNA-seq approaches in Xenopus tropicalis gastrulae and find that occupancy of the corepressor, TLE/Groucho, is a better indicator of tissue -specific cis-regulatory modules (CRMs) than the coactivator p300 , during early embryonic stages. (xenbase.org)
  • The role of Cyp26 during RA utilization and the anteriorization of the neuroectoderm during the early embryonic stages of Xenopus is also unclear. (molcells.org)
  • To identify new potential key factors of the organizer, we performed complementary DNA (cDNA) microarray screening using organizer-mimicking Xenopus laevis tissue. (bvsalud.org)
  • According to a generally accepted mechanism, inhibition of BMP signaling generates anterior neural tissue as a default pathway ( Nieuwkoop, 1952 ). (molcells.org)
  • These findings demonstrated that Tmem150b functions as a novel membrane regulatory factor of BMP signaling with antagonistic effects, contributing to the understanding of regulatory molecular mechanisms of organizer axis function. (bvsalud.org)
  • Surprisingly, wild-type posterior visceral endoderm (PVE) in midgut- and hindgut-deficient tetraploid chimera rescues the posterior body axis truncation, indicating that the PVE is important for tail organizer formation. (biologists.com)
  • Larval equivalent stages of ascidians and vertebrates have resembling structures such as a central notochord (embryonic structure forming the vertebral colomn in vertebrates), a dorsal neural tube and flanking muscles. (uibk.ac.at)
  • Forexample, the Spemann organizer secretes Cerebus and insulin-like growth factorswhich inhibits Wnt and BMP signals in order to form the head region, while theposterior region is established by a Wnt and BMP signal gradient (Neurulationand the Notochord, 2010). (wallaceandjames.com)
  • A hallmark of Spemann organizer function is its expression of Wnt antagonists that regulate axial embryonic patterning. (elifesciences.org)
  • However, it is unclear whether anterior neural tissue produced by inhibition of BMP is simply generated by a default pathway of pre-existing intracellular molecules or by the newly expressed posterior modifier inhibition molecules. (molcells.org)
  • During the development process, vimentin is exchanged for new, tissue-specific IFPs. (thermofisher.com)
  • We demonstrate a role for a member of the Ssbp family in kidney organogenesis and provide evidence of a fundamental function for the Ldb1-Lhx1-Ssbp transcriptional complexes in embryonic development. (bvsalud.org)
  • German biologist, Christiane Nusslein-Volhard is renowned for elucidation of the genetic control of embryonic development of fruit flies. (wepapers.com)
  • TRD3 Mutationsof the FGF signaling pathway can cause major embryonic abnormalities and caninterfere with or arrest proper development. (wallaceandjames.com)
  • The Spemann organizer instigates development of the central nervous systemby inducingcells into becoming neural TRD8 platecells, which will fold together to form the neural tube and eventually thespinal chord, forbrain, midbrain, and hindbrain. (wallaceandjames.com)
  • builds a bridge to today's molecular and cellular understanding self-organization in developing systems, including gastrulation, stem cell organoids, morphogen gradients, invertebrate systems, Evo-Devo, and emerging fields deriving from the discovery of embryonic induction. (elsevier.com)
  • As Spemann and others would soon recognize, however, induction was a much broader phenomenon. (mbl.edu)
  • The discovery that some well-known growth factors have inducing activity in embryogenesis has accelerated our understanding of embryonic induction. (ehu.eus)
  • Thus, four main themes recur: (a) investigations of the patterns of movement of embryonic cells, (b) analysis of cell fate and developmental potential, (c) the role of induction in cell fate allocation, and (d) the acquisition of more refined regional pattern ('regionalization') beyond differentiation into the major classes of cell types. (silverchair.com)
  • The process of gastrulation is linked to determination of mesodermal cell fates, such that patterning of tissue fates and patterning of cell behavior are interconnected. (nature.com)
  • Activin induces animal caps to differentiate into various mesodermal and endodermal tissues, including beating hearts, in a dose-dependent fashion. (ehu.eus)
  • The conference will link to recent advances on organizing principles in gastruloids and organoids - from stem cells to synthetic organizers. (elsevier.com)
  • In this study they used murine embryonic stem cells and transfected with Ngn2 expression and after five days post transfection they observed that cells expressed Tuj1 and MAP2 (both are neuronal markers). (bartleby.com)
  • Ectopic expression of Ngn2 was sufficient to form mature neurons from embryonic stem cells[5]. (bartleby.com)
  • Embryonic stem cells have been identified by scientist as a type of stem cell that can advance regenerative medicine. (bartleby.com)
  • However, the greatest potential embryonic stem cells presents are its ability to change into any of the more than 200 different cell types in the body. (bartleby.com)
  • During this time, Spemann continued his experiments with transplantation of tissues in amphibians (frogs and salamanders). (mbl.edu)
  • Tetraploid rescue experiments reveal that lack of β-catenin in the anterior visceral endoderm results in defects in head organizer formation. (biologists.com)
  • 2020), Sox17 and β-catenin co-occupy Wnt-responsive en. (xenbase.org)
  • Our findings establish Sox17 as a tissue -specific modifier of Wnt responses and point to a novel paradigm where genomic specificity of Wnt/β-catenin transcription is determined through functional interactions between lineage-specific Sox TFs and β-catenin/Tcf transcriptional complexes. (xenbase.org)
  • This analysis yielded a list of prospective organizer genes, and we determined the role of six-transmembrane domain containing transmembrane protein 150b (Tmem150b) in organizer function. (bvsalud.org)
  • We show that PTPRK acts via the transmembrane E3 ubiquitin ligase ZNRF3, a negative regulator of Wnt signaling promoting Wnt receptor degradation, which is also expressed in the organizer. (elifesciences.org)
  • Phenotypes of loss- and gain-of-function of head organizer TFs in frogs. (xenbase.org)
  • Deficiency of ptprk increases Wnt signaling, leading to reduced expression of Spemann organizer effector genes and inducing head and axial defects. (elifesciences.org)
  • Chordin blocks BMP signaling by preventing BMP from interacting with cell surface receptors, which inhibits the formation of epidermis and promoting the formation of neural tissue. (wikipedia.org)
  • The first is the surface ectoderm, which gives rise to tissues on the outer surface of the body like epidermis, hair, and nails. (asu.edu)
  • Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
  • Blocking of BMPs in animal cap explants causes the formation of anterior neural tissues as a default fate. (molcells.org)
  • In animal cap explants, however, xCyp26c morpholinos did not alter anterior-to-posterior neural tissue formation. (molcells.org)
  • While all vertebrates are capable of some types of tissue regeneration, most, including humans, have lost the ability to regenerate whole structures such as limbs ( epimorphic regeneration ), [ 1 ]. (biomedcentral.com)
  • The major theme will be devoted to describe and analyze the plant ecological adaptations, plant growth and anatomy, Animal dissection, structure and function of tissues, plants and animals diversity. (uaeu.ac.ae)
  • Ectoderm is one of three germ layers-groups of cells that coalesce early during the embryonic life of all animals except maybe sponges, and from which organs and tissues form. (asu.edu)
  • Occupancy of tissue -specific cis-regulatory modules by Otx2 and TLE/Groucho for embryonic head specification. (xenbase.org)
  • Here we identify the tumor suppressor Protein tyrosine phosphatase receptor-type kappa (Ptprk), as a Wnt inhibitor of the Spemann organizer. (elifesciences.org)
  • The neural crest helps form many of the bones and connective tissues of the head and face, as well as parts of the peripheral nervous system. (asu.edu)
  • Using these devices, Otx2 and other head organizer TFs (for example, Lim1/Lhx1 (activator) or Goosecoid (repressor)) are able to upregulate or downregulate a large battery of target genes in the head organizer . (xenbase.org)
  • This led to the concept of two organizing centers: the head and the trunk-tail organizers. (silverchair.com)
  • 2014), Occupancy of tissue-specific cis-regulatory mod. (xenbase.org)
  • We propose that quantitative experimental embryology offers essential ways to explore the reaction of cells and tissues to targeted cell addition, removal, and confinement. (mdpi.com)
  • Activin, in combination with retinoic acid, also induces the formation of the pronephros, a primitive embryonic kidney. (ehu.eus)
  • In his article "On the Anatomy and Affinities of the Family of the Medusae," Huxley compared the anatomy of the jellyfish family and recognized that the two tissue layers he saw in the body plan of the adult jellyfish corresponded with, or were homologous to, the layers in the embryo of the chick that Pander had described. (asu.edu)
  • Furthermore, the activin-induced animal caps organize heads or trunk-and-tails in exactly the same manner as the organizer. (ehu.eus)
  • Tmem150b was expressed in the organizer region and induced by Activin/Nodal signaling. (bvsalud.org)