ReactivityPrimary antibodiesIgG1ProteinsELISAEpitopeIsotype controlRecognizesSensitivityMAbsReactsConcentrationMouseAscitesStabilizes lysosomal membranes and suppressesAntigenFITCPurificationAntigensDetectsDetectionPeptidesAssaysMurinePolyclonalTherapeutic antibodyClonesConjugatesDilutionHumanCharacterizationEffector functionsRRIDEpitopesBlotImmuneVitroPhage displayProtein encoded by this geneEndogenousFlow CytometryAutoimmune diseasesPharmaceuticalInhibitors
Reactivity7
- We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
- This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
- In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
- Diaclone supplies both in vitro and in vivo produced monoclonal and biologically active antibodies, depending on which method generates the best quality, specificity and reactivity for successful and reproducible results. (diaclone.com)
- Aydintug K, Inzana TJ, Letonja T, Davis WC, Corbeil LB. Cross-reactivity of monoclonal antibodies to Escherichia coli J5 with heterologous gram-negative bacteria and extracted lipopolysaccharides. (linscottsdirectory.com)
- The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88. (lsbio.com)
- The peptide sequence is conserved in mammals, birds, and amphibians, giving the antibody broad species reactivity. (cytoskeleton.com)
Primary antibodies1
- The negative controls are HRP-Conjugated Anti-Rabbit IgG antibody and HRP- Conjugated Anti-Mouse IgG antibody alone in different wells (without the primary antibodies). (antikoerper-online.de)
IgG13
- Bevacizumab is a humanized IgG1 monoclonal antibody that targets VEGF, a signaling protein that promotes the growth of new blood vessels. (invivogen.com)
- The Anti-Spike RBD clone CR3022 is a SARS-CoV neutralizing antibody that was obtained from the screening of an antibody-phage library and converted to a human IgG1 format [1]. (invivogen.com)
- Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection). (enzolifesciences.com)
Proteins13
- A survey of various species by immunoblot analysis with several antibodies revealed little if any epitope relatedness among microsomal proteins from rats, mice, rabbits, hamsters, squirrel monkeys, guinea pigs, or humans. (nih.gov)
- This antibody reacts to lysine-acetylated proteins. (sigmaaldrich.com)
- Monoclonals can be used directly to bind to surface proteins on cancer cells to kill or inhibit their growth," explains Dr. Moran. (labmanager.com)
- FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. (anobase.org)
- Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. (anobase.org)
- Monoclonal antibody therapy is a form of immunotherapy that uses monoclonal antibodies (mAbs) to bind monospecifically to certain cells or proteins. (wikipedia.org)
- We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
- Diaclone Monoclonal Antibodies are highly specific, high quality mouse monoclonal antibodies for the detection of a broad range of human proteins. (diaclone.com)
- Rapid and comprehensive analysis of putative proteinous allergens ( allergenome ) by applying such a proteomic strategy … With allergenomics, we can not only detect and assign the putative allergens (proteins specifically interacting with IgE antibodies in a patient's blood) in a short time, but also analyze the quantitative and qualitative change of the antigens, depending on the surroundings and environmental conditions of an allergenic causative. (genomicglossaries.com)
- The antibody has been shown to recognize a wide range of SUMO-2/3-targeted proteins in HeLa cell lysate (Fig. 1B) and to detect sub-nanogram amounts of recombinant SUMO-2 (Fig. 1A). (cytoskeleton.com)
- Western blots of immunoprecipitated proteins were developed using 12F3 (A) or anti-TFII-I antibody (B). (A) Star (*) and circle (o) indicate heavy and light chains of antibodies. (cytoskeleton.com)
- Monoclonal mouse Strep -tag antibodies are used to detect recombinant proteins carrying the short Strep -tag affinity tag epitope with high specificity and sensitivity. (qiagen.com)
- Proteins can be detected with high specificity and sensitivity using mouse monoclonal Strep -tag or Anti·His antibodies. (qiagen.com)
ELISA5
- Monoclonal antibody ELISA for cotinine in saliva and urine of active and passive smokers. (nih.gov)
- These antibodies can be utilized over an extensive application range including Flow Cytometry, Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA, ELISpot and Biological assays. (diaclone.com)
- The Anti-ETK (Phospho-Tyr566) antibody is highly specific for the phospho-peptide in comparison to the non- phospho peptide counterpart, through an ELISA. (antikoerper-online.de)
- Application Instructions These antibodies have been qualified for use in ELISA to detect human a1AC. (qedbio.com)
- To access the diagnostic accuracy of the developed kit, ELISA and PCR RVA positive and negative samples will be tested using the nanoparticle kit and the sensitivity/specificity of these methods will be compared. (who.int)
Epitope3
- Additionally, for complex biological matrices, antibody selection can be challenging since protein targets secreted from cells into cell culture media or serum may be cleaved or modified, altering epitope recognition-hence the benefit of using protease cocktail inhibitors. (genengnews.com)
- Epitope mapping has identified that the antibody recognizes a sequence/structure within the peptide CQIRFRFDGQPINE. (cytoskeleton.com)
- The importance of several of these conserved residues was supported by their presence in a mouse monoclonal antibody with a known structure and the same epitope specificity. (lu.se)
Isotype control1
- An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
Recognizes1
- The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q. (origene.com)
Sensitivity4
- IgG fraction Monoclonal Mouse Anti-Digoxin may be used either as direct conjugates, or for more sensitivity, they can be used unconjugated followed by a conjugated anti-mouse IgG (H+L) for signal enhancement . (jacksonimmuno.com)
- T1", "T2" and "T3") and form a visible line as the antibody-antigen-antibody gold particle complex with high degree of sensitivity and specificity. (who.int)
- [ 1 , 2 ] Nucleic acid amplification test (NAAT) has surpassed this as the criterion standard, with sensitivity ranging from 85-95% with 99-100% specificity. (medscape.com)
- In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)
MAbs3
- Therapeutic monoclonal antibodies (mAbs), for example, have emerged as a promising treatment strategy. (labmanager.com)
- InvivoGen provides a family of Anti-Spike RBD CR3022 -derived monoclonal antibodies (mAbs) in multiple human and mouse isotypes. (invivogen.com)
- Therapeutic monoclonal antibodies (MAbs) have been found to be highly effective agents in the treatment of immunological disorders with a high level of success due to their structural specificity and low toxicity. (lu.se)
Reacts3
- The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC). (anobase.org)
- The antibody MEM-143 reacts with a protein determinant of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). (lsbio.com)
- Mouse monoclonal antibodies specific to Zaire Ebola virus GP, NP and VP40 - colloid gold conjugate reacts with the Zaire Ebola virus in the specimen. (who.int)
Concentration3
- Passive monoclonal antibody therapy can ensure consistent antibody concentration, and can control for adverse reactions by stopping administration. (wikipedia.org)
- The binding sites of the labeled antibody become occupied partly by the sample analyte (depending on its concentration) and partly by the ruthenium-labeled hapten to form the respective immunocomplexes. (cdc.gov)
- Monoclonal antibody storage conditions, and concentration effects on immunohistochemical specificity. (nih.gov)
Mouse16
- In an early study, they fused mouse myeloma and spleen cells from an immunized donor to produce cell lines that secreted anti-sheep red blood cell antibodies. (labmanager.com)
- Once they are considered a therapeutic lead, these mouse antibodies must be humanized (by genetic engineering) unless they are made in a humanized mouse, which allows more rapid progress to a potential drug stage," explains Dr. Thomas Moran, a professor of microbiology at the Icahn School of Medicine at Mount Sinai and the director of the Center for Therapeutic Antibody Development. (labmanager.com)
- Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. (anobase.org)
- Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. (anobase.org)
- The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. (origene.com)
- The antibody is a mouse monoclonal antibody raised against GZMK. (biocheminfo.org)
- LENG1 antibody LS-C337402 is an unconjugated mouse monoclonal antibody to human LENG1. (lsbio.com)
- Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. (abcam.com)
- The Anti-GAPDH antibody is a mouse monoclonal antibody. (antikoerper-online.de)
- It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. (antikoerper-online.de)
- 4) Positive and Negative Controls: Mouse Anti-GAPDH antibody (included) should be used to detect the internal positive controls for normalization of OD values of the target protein. (antikoerper-online.de)
- Anti-SUMO2/3 antibody is a SUMO-2/3 mouse monoclonal antibody that is part of the Signal-Seeker™ product line. (cytoskeleton.com)
- Anti-SUMO-2/3 mouse monoclonal antibody was raised against full-length recombinant SUMO-2 protein (Uniprot: P61956) combined with a proprietary mix of peptides that include CQIRFRFDGQPINE. (cytoskeleton.com)
- Mouse monoclonal antibody raised against a full length recombinant CESK1. (abnova.com)
- Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions - guaranteeing absence of viruses, mycoplasma, and contaminating immunoglobulins. (qiagen.com)
- Several of these corresponding residues in the mouse monoclonal antibody are known to interact with the antigen. (lu.se)
Ascites3
- The in vivo monoclonal antibody production process (Ascites production) is recognised as being painful for animals. (diaclone.com)
- Only a few monoclonal antibody clones with better specificity & productivity from ascites production have been conserved in the catalogue, in accordance with the strict regulations of veterinary agencies. (diaclone.com)
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Stabilizes lysosomal membranes and suppresses2
- It stabilizes lysosomal membranes and suppresses lymphocytes and antibody production. (medscape.com)
- Prednisone stabilizes lysosomal membranes and suppresses lymphocyte and antibody production. (medscape.com)
Antigen8
- Several antibodies were antigen-specific, others reacted with additional members of the 2C subfamily, and other monoclonal antibodies recognized cytochromes P450 from the 2E, 2B, 2A, and 1A subfamilies. (nih.gov)
- Dr. Fox explains this process: "We receive the antigen from the person who wishes to develop a monoclonal antibody. (labmanager.com)
- Supernatant [fluid] is taken from the hybridomas, and the investigator screens for the monoclonal antibody of interest that will recognize a specific antigen and is appropriate for their application. (labmanager.com)
- Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. (anobase.org)
- Monoclonal antibodies target the CD20 antigen expressed on the surface of pre-B and mature B-lymphocytes. (medscape.com)
- See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. (lsbio.com)
- In conclusion, critical residues important for maintaining a human antigen-specific binding site during the process of in vitro antibody evolution were defined. (lu.se)
- A rotavirus diagnostic kit will be developed using nanotechnology involving lactoferrin (LF), magnesium chloride, silicon, and monoclonal antibody to the VP6 gene, a group-specific antigen found in all RV that cause disease in humans. (who.int)
FITC1
- The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface. (anobase.org)
Purification1
- The two-step affinity purification system, using the Strep- tag Antibody for detection, is highly suited for applications where high purity is at a premium or is difficult to achieve. (qiagen.com)
Antigens5
- The four known IgG subclasses are involved in antibody-dependent cellular cytotoxicity.Antibodies are a key component of the adaptive immune response, playing a central role in both in the recognition of foreign antigens and the stimulation of an immune response to them. (wikipedia.org)
- The advent of monoclonal antibody technology has made it possible to raise antibodies against specific antigens presented on the surfaces of tumors. (wikipedia.org)
- Additionally, adverse reactions from these antibodies may occur because of long-lasting response to antigens. (wikipedia.org)
- We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
- When targeted monoclonal antibodies are cloned from immune cells, a mass of high-affinity molecules are released that can bind vital antigens to everything from cancer cells to pathogens. (element.com)
Detects2
- The antibody detects endogenous Collagen II protein. (biocheminfo.org)
- For immunohistochemistry, this antibody only detects actin in smooth muscle and not cardiac muscle. (abcam.com)
Detection2
- A HRP-labelled anti-hIgG1 antibody (1/1000 dilution) and the HRP substrate OPD (o-phenylenediamine dihydrochloride) were used for the detection step. (invivogen.com)
- Examination of direct smears for acid fast bacilli is the widely used method for the detection of mycobacteria, but lacks the desired specificity and relatively large number of bacteria is needed for detection. (who.int)
Peptides1
- The most promising Ags were submitted to a more in-depth biophysical investigation of their structure and conformation stability, and for their capacity to interact with neutralising antibodies (Abs) and peptides. (europa.eu)
Assays2
- Functional assays (Ref.1): Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. (origene.com)
- Stereospecific monoclonal antibodies to nicotine and cotinine and their use in enzyme-linked immunosorbent assays. (nih.gov)
Murine5
- citation needed] Four major antibody types that have been developed are murine, chimeric, humanised and human. (wikipedia.org)
- citation needed] Initial therapeutic antibodies were murine analogues (suffix -omab). (wikipedia.org)
- citation needed] Initially, murine antibodies were obtained by hybridoma technology, for which Jerne, Köhler and Milstein received a Nobel prize. (wikipedia.org)
- However the dissimilarity between murine and human immune systems led to the clinical failure of these antibodies, except in some specific circumstances. (wikipedia.org)
- A murine monoclonal antibody with broad specificity for occupationally relevant diisocyanates. (cdc.gov)
Polyclonal2
- As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1). (origene.com)
- The Anti-ETK (Phospho-Tyr566) antibody is a rabbit polyclonal antibody. (antikoerper-online.de)
Therapeutic antibody2
- Chimeric and humanized antibodies have generally replaced them in therapeutic antibody applications. (wikipedia.org)
- Within the next decade, the global therapeutic antibody market is expected to double, partly due to the addition of emerging technologies such as phage display. (element.com)
Clones2
- With over 30 years of experience Diaclone is respected for its excellence in Monoclonal Antibody development and our highly respected B-clones ('B-' for Besancon) are utilized across the Life Science industry in research, clinical and discovery applications by research, pharmaceutical and manufacturing organizations worldwide. (diaclone.com)
- Using CDR shuffling, residues important for the assembly of mucin-1 specific paratopes were defined by random recombination of the complementarity determining regions derived from a set of mucin-1 specific clones, previously selected from an antibody fragment library. (lu.se)
Conjugates3
- The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. (biotium.com)
- Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. (biotium.com)
- For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). (biotium.com)
Dilution2
- A 3-fold serial dilution of the Anti-Spike-RBD hIgG1 (red curve) or of the Anti-βGal hIgG1 control antibody (grey curve) was performed for the capture step. (invivogen.com)
- No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. (enzolifesciences.com)
Human6
- Very recently, human monoclonal antibodies were approved for use on patients with COVID-19. (frontiersin.org)
- The human monoclonal antibodies used in this study are almost identical with these approved antibodies. (frontiersin.org)
- Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants. (invivogen.com)
- , 2020. Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody. (invivogen.com)
- This antibody has specificity for Human IL13RA1/CD213a1. (thermofisher.com)
- Specificity These antibodies specifically recognize human serum alpha-1-antichymotrypsin. (qedbio.com)
Characterization5
- The CS-35 antibody is widely used in the characterization of glycans containing D-arabinofuranose residues, in particular polysaccharides present in the mycobacterial cell wall. (uni-luebeck.de)
- A detailed understanding of the combining site of this antibody and the measurement of its binding to different ligands is of interest as this knowledge will have implications in the characterization of arabinofuranose-containing glycoconjugates that are increasingly recognized as important biological molecules. (uni-luebeck.de)
- Monoclonal antibody therapies have significant potential, but they also present significant risks, reinforcing the need for a partnership with trusted a CDMO that excels in monoclonal antibody characterization and analysis. (element.com)
- At Element, we're always one step ahead, exceling in QC-minded monoclonal antibody characterization and analysis. (element.com)
- For more information about Element's monoclonal antibody (mAb) analysis and characterization services or to request a quote, contact us today. (element.com)
Effector functions1
- These antibodies have: a short half-life in vivo (due to immune complex formation), limited penetration into tumour sites and inadequately recruit host effector functions. (wikipedia.org)
RRID2
- The following product was used in this experiment: Neuropilin 1 Recombinant Rabbit Monoclonal Antibody (042) from Thermo Fisher Scientific, catalog # MA5-43129, RRID AB_2912266. (thermofisher.com)
- The following product was used in this experiment: IL13RA1 Monoclonal Antibody (6E6E1C3) from Thermo Fisher Scientific, catalog # MA5-30529, RRID AB_2786249. (thermofisher.com)
Epitopes2
- A minimum of seven spatially distinct epitopes on cytochrome P450h were defined by the panel of antibodies. (nih.gov)
- Furthermore, an explanation for the observed restricted germline gene usage in certain antibody responses against protein epitopes is provided. (lu.se)
Blot1
- Western Blot analysis of CESK1 expression in transfected 293T cell line by CESK1 monoclonal antibody (M01), clone 4G6. (abnova.com)
Immune4
- They] can work by recruiting immune elements, delivering toxins, producing CAR-T cells, or as bispecific antibodies that recruit T or NK cells to kill the cancer cells. (labmanager.com)
- Monoclonal antibodies can be acquired in the immune system via passive immunity or active immunity. (wikipedia.org)
- The advantage of active monoclonal antibody therapy is the fact that the immune system will produce antibodies long-term, with only a short-term drug administration to induce this response. (wikipedia.org)
- Monoclonal antibody therapy can aid the immune system because the innate immune system responds to the environmental factors it encounters by discriminating against foreign cells from cells of the body. (wikipedia.org)
Vitro1
- MORE THAN 98% of Diaclone Monoclonal antibodies are produced in vitro ! (diaclone.com)
Phage display1
- Access to live virus stocks would be needed during the initial stages of monoclonal antibody production or if, for example, phage display systems were to be developed. (who.int)
Protein encoded by this gene1
- The protein encoded by this gene belongs to the dual specificity protein phosphatase family. (origene.com)
Endogenous1
- Endogenous testosterone released from the sample by ANS (8-anilino-1-naphthalene sulfonic acid) and norgestrel competes with the added testosterone derivative labeled with ruthenium complex for the binding sites on the biotinylated antibody. (cdc.gov)
Flow Cytometry1
- This antibody was developed for use in flow cytometry. (bdbiosciences.com)
Autoimmune diseases1
- Monoclonal antibody therapy may prove to be beneficial for cancer, autoimmune diseases, and neurological disorders that result in the degeneration of body cells, such as Alzheimer's disease. (wikipedia.org)
Pharmaceutical1
- Pharmaceutical and biotechnology companies have developed monoclonal antibodies that neutralize SARS-CoV-2. (forbes.com)
Inhibitors1
- All of the antibodies were screened as potential inhibitors of cytochrome P450h-mediated testosterone hydroxylation in a reconstituted system. (nih.gov)