Specificity cells mice after antigen. Medical search. Frequent questions

In 1973 he described, with Edward Cohen, a method of producing antibodies involving human-mouse hybrid cells, or hybridomas. (wikipedia.org)
The antibody producing cells did not survive long and the antigens that the antibodies targeted remained unknown. (wikipedia.org)
In 1975, Georges Köhler, César Milstein, and Niels Kaj Jerne, succeeded in making hybridomas that made antibodies to known antigens and that were immortalized. (wikipedia.org)
Despite their efficiency, adoptive T-cell therapies show unique toxicities, which are distinct from those seen with conventional chemotherapies, monoclonal antibodies (mAbs), and small-molecule-targeted therapies. (nature.com)
See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. (lsbio.com)
Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. (fishersci.com)
The rationale of this study was to determine the myelotoxicity in nude mice of the α-emitter 211 At conjugated to monoclonal antibodies (mAbs) and to compare the effect with an electron emitter, 99m Tc, and external irradiation from a 60 Co source, for estimation of the relative biological effectiveness (RBE). (snmjournals.org)
A panel of 11 murine monoclonal antibodies directed against dengue type 2 was evaluated for antigen specificity by dot immunobinding assay and Western blot analysis and for in vitro and in vivo biological activities. (ajtmh.org)
Of the neutralizing monoclonal antibodies, five passively protected mice in vivo against lethal intracerebral dengue 2 challenge. (ajtmh.org)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Conventionally, monoclonal antibodies (mAbs) used in medical research were derived from mouse cells. (uksfbooknews.net)
However, in recent years, rabbit monoclonal antibodies (rAb) have emerged as an innovative alternative to traditional mAbs, offering significant advantages in terms of specificity, affinity, and their ability to bind to novel antigens. (uksfbooknews.net)
In conclusion, rabbit monoclonal antibodies represent an exciting development in medical research, offering numerous advantages over traditional mouse-derived mAbs. (uksfbooknews.net)
secreted version: antibodies) and T cell receptor (TCR), respectively. (nature.com)
The data show that arthritic DQ8 mice treated with anti-CD20 antibody in therapeutic protocols show milder disease severity in females as compared to males, which is associated with decreased antibodies to citrullinated proteins and reduced levels of IL-23 and CCL5. (elsevierpure.com)
By means of immunohistochemistry and double-labeling studies using transgenic mouse lines as well as Western blot analyses, it was demonstrated that the newly generated antibodies specifically recognized the receptor proteins. (jneurosci.org)
A second signal for T cell mitogenesis provided by monoclonal antibodies CD45 (T200). (absoluteantibody.com)
B cells, the immune cells that produce antibodies, each recognize and bind a specific antigen through their B cell receptor (BCR). (fredhutch.org)
Herein we used a single bicistronic lentiviral vector to develop a new combination immunotherapy that consists of human anti-carbonic anhydrase IX (CAIX)-targeted chimeric antigen receptor (CAR) T cells engineered to secrete human anti-programmed death ligand 1 (PD-L1) antibodies at the tumor site. (oncotarget.com)
These armed second-generation CAR T cells empowered to secrete human anti-PD-L1 antibodies in the ccRCC milieu to combat T cell exhaustion is an innovation in this field that should provide renewed potential for CAR T cell immunotherapy of solid tumors where limited efficacy is currently seen. (oncotarget.com)
CRA treatment attracted an abundance of B cells into the tumor consequently enhancing the anti-tumor antibodies in sera and inducing a class-switch. (bvsalud.org)
CONCLUSIONS: CRA regulated T cell activity resulting in B cell activation and triggering of anti-tumor antibodies to impede CRC progression. (bvsalud.org)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
Two monoclonal antibodies, TAL-1B5 and TAL-3C3, specific for human Ia alpha-chain subunits have been produced by fusing P3/NSI/1-Ag4-1 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified alpha-chains. (ox.ac.uk)
The epitope specificity of the antibodies in relapsing polychondritis differs from those in RA, suggesting different mechanisms for formation and pathophysiologic roles. (medscape.com)

CD62P antibody LS-B3656 is an unconjugated mouse monoclonal antibody to human CD62P (SELP / P-Selectin). (lsbio.com)
Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: This P3.6.2.8.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P3.6.2.8.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. (fishersci.com)
Histograms show the level of SSTR2-specific antibody binding to wild-type Jurkat T cells (0%, top) and Jurkat T cells transduced with increasing virus titers. (jci.org)
B ) Level of SSTR2-specific antibody binding to SSTR2-transduced Jurkat T cells with and without preincubation with 1 μM octreotide (37°C, 30 minutes). (jci.org)
IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. (anobase.org)
The variable region of IgG antibody is specific to antigens and is highly conserved. (anobase.org)
Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. (anobase.org)
Anti-Mouse IgG (whole molecule)-Peroxidase antibody may be used in direct ELISA at a working antibody dilution of 1:40,000. (anobase.org)
Anti-Mouse IgG (whole molecule)-Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin array assay. (anobase.org)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
Human IgG antibody Laboratories manufactures the is igm done for antbody or antigen reagents distributed by Genprice. (iowaodes.com)
C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.25 µg of CD3 Monoclonal Antibody, Super Bright 600 (right). (thermofisher.com)
Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. (thermofisher.com)
Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
Rabbits have a distinct advantage over mice in immunological research because their immune systems can generate more diverse and robust antibody responses. (uksfbooknews.net)
In addition, rabbits can be immunized with smaller amounts of antigen, which minimizes the risk of side effects that may occur when using larger amounts to stimulate the immune response required for antibody production. (uksfbooknews.net)
However, the substrates of the immunofluorescence assay were not human but mouse brain tissues, which could influence the sensitivity and specificity of the antibody. (go.jp)
In the present study, we have established human cell lines that stably express human AQP4 and used these cells to detect and titrate anti-AQP4 antibody present in the sera of patients with NMO by immunofluorescence assay. (go.jp)
The staining pattern of AQP4-expressing cells treated with each serum of these eight NMO patients corresponded to that with a commercially available anti-AQP4 antibody. (go.jp)
CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. (exbio.cz)
Separation of K562 cells stained using anti-human CD90 (Ber-H8) PE antibody (concentration in sample 5 μg/ml, red) from unstained K562 cells (black) in flow cytometry analysis (surface staining). (exbio.cz)
Anti-CD90 In Vivo Antibody - Low Endotoxin (HK2.1) recognizes the Thy-1.1 and Thy-1.2 alloantigens of all mouse strains tested. (ichor.bio)
This antibody is reported to stimulate mouse T-cell proliferation. (ichor.bio)
LAMP1/CD107a was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human LAMP1/CD107a Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC7985G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). (rndsystems.com)
The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
This antibody was raised by immunizing mice with stimulated human leukocytes. (absoluteantibody.com)
For instace, one group utilized EDU-2 antibody to identify CD4-posotive cells via a modified flow cytometric assay in order to develop efficient and cost-effective method for HIV infection monitoring (Barbesti et al. (absoluteantibody.com)
2005). Furthermore, EDU-2 antibody was also used to differentiate between different subsets of dendritic cells (Maniecki et al. (absoluteantibody.com)
The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody. (absoluteantibody.com)
Find blocking peptides and proteins for use as quality controls to confirm antibody specificity. (novusbio.com)
CRA antisera induced cytotoxicity including antibody-dependent cell cytotoxicity, phagocytosis, and complement-dependent cytotoxicity. (bvsalud.org)
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. (abcam.com)
Antibody JOVI-3 is specific for the human V beta 3 TCR gene product and reacts with 1-10 of T cells in normal human blood (1, 5). (ancell.com)
Antibody JOVI-3 is mitogenic for T cells expressing TCR V beta 3. (ancell.com)
Biotin Anti-Mouse CD117 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and immunoprecipitation assays. (southernbiotech.com)
2D-NEPHGE/SDS-PAGE analysis of TAL-1B5 immunoprecipitates from [35S]-methionine biosynthetically labelled cells revealed the presence of a number of alpha-chain spots in association with beta-chain products of three previously described loci (beta-1, beta-2, beta-3) suggesting that this antibody recognizes an antigenic site common to those human Ia alpha-chains so far identified. (ox.ac.uk)
Therefore, the use of a combination of antibody- and antigen-detection methods for the diagnosis of chlamydiosis is recommended, particularly when only one bird is tested. (cdc.gov)
The evidence for an autoimmune etiology includes pathological findings of infiltrating T cells, the presence of antigen-antibody complexes in affected cartilage, cellular and humoral responses against collagen type II and other collagen antigens, and the observation that immunosuppressive regimens most often suppress the disease. (medscape.com)

In direct ELISAs, less than 1% cross-reactivity with recombinant mouse LAMP1 is observed. (rndsystems.com)
In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6, recombinant rat EphB1, and rmEphB3 is observed. (rndsystems.com)
To eliminate auto-immune reactivity, several levels of checkpoints throughout B cell development prevent self-reactive B cells from developing or becoming activated. (fredhutch.org)
However, this system can also backfire, as some B cells specific for a foreign antigen may also display low-level cross-reactivity for self-antigen, leading to their deletion or hindrance. (fredhutch.org)
Because these B cells have never encountered the antigen for which they are specific, any Nur77 eGFP expression, and therefore BCR stimulation, can be attributed to cross-reactivity to a self-antigen. (fredhutch.org)
The degree of cross-reactivity with self-antigen varied between B cells specific for various model antigens, but was not increased specifically in model antigen-specific B cells compared to polyclonal B cells of unknown specificities. (fredhutch.org)
Because model antigens are convenient tools that may not be representative of antigens derived from human pathogens, the authors next repeated this experiment with naïve B cells specific for HIV, respiratory syncytial virus (RSV) and influenza virus, and again found the same level of cross-reactivity to self-antigen. (fredhutch.org)
Together, these experiments showed that a range of cross-reactivity to self is observed within B cells of a given specificity, but that cross reactivity to self is not limited to B cells specific for foreign antigens. (fredhutch.org)
To determine if this lack of responsiveness corresponded to a reduced ability to proliferate after BCR stimulation, Nur77 eGFP -low, medium, and high B cells were identified as a proxy for increasing levels of cross-reactivity with self-antigen and incubated with anti-immunoglobulin to stimulate the BCR. (fredhutch.org)
Based on these in vitro results, the authors moved to a mouse model to determine if cells displaying high self-reactivity would have dampened antigen responses in vivo . (fredhutch.org)
They found that Nur77 eGFP -low and high cells proliferated least compared to Nur77 eGFP -medium donor cells, suggesting that self-reactivity acts as a secondary signal to tune B cells responses to antigen and that a moderate level of self-reactivity corresponds with optimal B cell function. (fredhutch.org)
Moderate self-antigen reactivity corresponds with optimal B cell activity against foreign antigens. (fredhutch.org)
As in mouse cells, human B cells specific for foreign antigens did not express differential amounts of IgM compared to bulk B cells, suggesting that cross-reactivity to self is a B cell-wide trait not limited to B cells of certain specificities. (fredhutch.org)
The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)

Genetically engineered T cells expressing a chimeric antigen receptor (CAR) are rapidly emerging a promising new treatment for haematological and non-haematological malignancies. (nature.com)
SSTR2-based PET was applied to ACT of chimeric antigen receptor (CAR) T cells targeting intercellular adhesion molecule-1, which is overexpressed in anaplastic thyroid tumors. (jci.org)
Much more advanced in clinical development than T-cell receptor transgenic cells are cellular therapies using so-called CAR-T (chimeric antigen receptor) cells, which have already been approved for the treatment of various leukemias and lymphomas. (dkfz.de)

Fortunately, rAbs have been shown to have higher affinity and specificity for their target antigen, making them more effective in treating resistant cancers. (uksfbooknews.net)
The target antigen of NMO-IgG was recently identified as aquaporin-4 (AQP4) water channel protein, which is mainly expressed in brain and spinal cord. (go.jp)

The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. (exbio.cz)
Proliferation was measured by a cell dye that dilutes as cells divide, reavealing that Nur77 eGFP -high B cells proliferated least in response to BCR signaling, while low Nur77 eGFP -expressing B cells proliferated the most. (fredhutch.org)
Choose from hundreds of bioactive proteins for functional assays including cell proliferation assays, enzyme assays, or functional ELISAs. (novusbio.com)
A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. (southernbiotech.com)
In: Chemically induced cell proliferation: Implications for risk assessment. (cdc.gov)
Ozone exposure impairs antigen-specific immunity but activates IL-7-induced proliferation of CD4(-)CD8(-) thymocytes in BALB/c mice. (cdc.gov)

PCRs may provide additional sensitivity with no loss of specificity. (cdc.gov)
The BD FACSymphony™ A3 Cell Analyzer improves sensitivity to enable you to identify and analyze rare cell types and events. (bdbiosciences.com)
Initial evaluations determined that SSTR2-expressing T cells were detectable at low densities with high sensitivity and specificity. (jci.org)
Additionally, rAbs offer superior sensitivity and specificity in detecting low-abundance targets such as cancer biomarkers or signaling proteins. (uksfbooknews.net)
Their superior sensitivity, specificity, and stability make them valuable tools for researchers seeking to identify potential therapeutic targets for diseases such as cancer and autoimmune disorders. (uksfbooknews.net)
When used with cloacal or fecal smears, the sensitivity and specificity of the test are questioned by some authorities. (cdc.gov)

These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
Natural killer (NK) cells are innate cytotoxic lymphocytes with adaptive immune features, including antigen specificity, clonal expansion and memory. (medchemexpress.com)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. (lsbio.com)
The number and inhibitory activity of CD4(+)CD25(+)FoxP3(+) regulatory T cells were analysed by histology, flow cytometry and in vitro mixed lymphocyte cultures. (uni-koeln.de)
Each BD fluorochrome optimized for flow cytometry has been conjugated to anti-human or mouse CD4 and is provided for PMT voltrations and instrument characterization. (bdbiosciences.com)
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10 6 cells in a suspension. (exbio.cz)
The phenotypes of B cell subsets in splenocytes and tumors from the CRA-treated mice were isolated and analyzed by flow cytometry. (bvsalud.org)

Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. (anobase.org)
The anti-PD-L1 IgG1 isotype, which is capable of mediating ADCC, was also able to recruit human NK cells to the tumor site in vivo . (oncotarget.com)

Recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. (lsbio.com)
Bunse's team then isolated from the blood of the vaccinated individuals those T cells that had been activated by NLGN4X and consequently carried a T cell receptor that specifically "recognizes" this glioblastoma-associated antigen. (dkfz.de)

Immunohistochemistry: LS-B3656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. (lsbio.com)

Human x mouse somatic cell hybrid clone secreting immunoglobulins of both parental types. (wikipedia.org)
Representative PMTV titration and measured stain index of PBMCs stained with clone SK3 mouse anti-human CD4 acquired at varying PMTVs in 25 V increments. (bdbiosciences.com)
Functional Assay: Anti-Mouse CD90 (Thy-1) (clone HK2.1) has been implicated in activation of T-cells. (ichor.bio)

By means of in vivo fate mapping, we found a striking variability of immune responses derived from individual CD8(+) T cells and show that robust acute and recall immunity requires the initial recruitment of multiple precursors. (nih.gov)
In this study, we have analyzed and compared three prostate-specific promoters (PSA, probasin, and MMTV LTR) for their specificity and activity both in vitro and in vivo . (hindawi.com)
Immunosuppression mediated by MICCop was investigated in vivo by daily assessment of clinical signs of paralysis and in in vitro restimulation assays of peripheral immune cells. (uni-koeln.de)
The Wells lab has a particular interest in extrinsic and intrinsic regulatory influences on CD8 T-cells and conducts basic research using in vivo and in vitro model systems. (edu.au)
For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)

2. Interferon γ cytokine secretion was determined by ELISPOT to assess PBMC response against known antigens in a preliminary experiment to approximate usable range for the following antigen specific PBMC assays. (helsinki.fi)
Indirect binding assays demonstrated that TAL-1B5 bound strongly to all human B lymphoblastoid lines tested and to CLLs, but only weakly to PBL-B cells and not to PBL-T cells or the T-cell lines Molt 4 and HSB-2. (ox.ac.uk)

Here, we recovered transcriptome and immune repertoire information for polyclonal T follicular helper cells following lymphocytic choriomeningitis virus (LCMV) infection, CD8+ T cells with binding specificity restricted to two distinct LCMV peptides, and B and T cells isolated from the nervous system in the context of experimental autoimmune encephalomyelitis. (nature.com)
In silico methods were successfully used to characterize previously identified HLA-restricted peptides and one previously identified immunogenic T-cell epitope. (helsinki.fi)
TCR's recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells (3, 4). (ancell.com)

A number of PSPs have been defined over the years that include, but not limited to, promoters of prostate-specific antigen (PSA), probasin (PB), mouse mammary tumor virus (MMTV LTR), prostate-specific membrane antigen (PSMA), human glandular kallikrein 2 (hK2), and prostatic steroid-binding protein C3. (hindawi.com)
These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. (exbio.cz)
CRA-induced antisera (designated CRA antisera) specifically recognized surface antigens on the plasma membrane of cancer cells. (bvsalud.org)
Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
These results indicated that gH is definitely a transmembrane protein and a substantial L-741626 fraction were readily surface-localized in transfected cells, while gL and gO may display the membrane-localization Rabbit Polyclonal to Cytochrome P450 4F3 pattern when interacting with gH by forming the heterotrimer complex (gH/gL/gO). (accessibletech4all.org)
They are able to activate complement, which causes selective deposition of C3b onto LPS molecules at sites distant from the bacterial cell membrane. (medscape.com)
This inhibits the formation of the membrane attack complex (C5b-C9), which prevents membrane damage and bacterial cell death. (medscape.com)

Treg, also called "natural" regulatory T cells, will be traced from their intra-thymic origin to the site of their action in peripheral lymphoid organs and tissues. (epa.gov)
Regulatory T cells, however also control infections, allergies and tolerance to transplanted tissues and this requires their induction in the periphery under conditions which are not yet fully understood. (epa.gov)
Designed to visualize the expression of LAMP1 by fluorescence microscopy for staining cells and tissues. (rndsystems.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Meanwhile, the ability of M4N to suppress energy metabolism prevented obesity in mice consuming HF diets, indicating that M4N has beneficial effects on normal tissues. (bvsalud.org)
For the analysis of GSLs, they are extracted from tissues and cells using organic solvents. (springer.com)
The specificity of autoimmune injury to cartilaginous tissues has led investigators to test the hypothesis that a cartilage-specific autoantibody is central to the pathogenesis of relapsing polychondritis. (medscape.com)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

A core feature of protective T cell responses to infection is the robust expansion and diversification of naïve antigen-specific T cell populations into short-lived effector and long-lived memory subsets. (nih.gov)
The use of this model may help to highlight the capacity of different M. tuberculosis antigens to induce a protective immune response, actually not necessarily embodied by an increased frequency of Antigen-specific effector memory T cells. (unicatt.it)

Humoral and cellular immune responses were then determined by ELISA and in vitro antigen restimulation assay. (uni-koeln.de)
Preliminary MTS assay is used to determine linear range and optimal time frame for the PBMC/cancer cell co-culture killing assay. (helsinki.fi)
Re-evaluation of 1,2-dimethylhydrazine in the mouse bone marrow micronucleus assay: Observation of a positive response. (cdc.gov)

Flow cytometric analysis of COS-7 cells using DNMT1 (D63A6) XP ® Rabbit mAb (PE Conjugate) (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) #5742 (dashed line). (cellsignal.com)

Antigen-detection ELISA is useful for diagnosis, particularly for severe cases ( 2 ). (cdc.gov)

Background: Dendritic cells (DCs) rendered suppressive by treatment with mitomycin C and loaded with the autoantigen myelin basic protein demonstrated earlier their ability to prevent experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis (MS). This provides an approach for prophylactic vaccination against autoimmune diseases. (uni-koeln.de)
Conclusion: We describe a clinically applicable cell therapeutic approach for controlling relapses in autoimmune encephalomyelitis by specifically silencing the deleterious autoimmune response. (uni-koeln.de)
The repertoire of Treg is clearly biased towards recognition of self-antigens, thereby potentially preventing autoimmune diseases such as gastritis and oophoritis. (epa.gov)
However, the stochastic nature of B cell development also produces B cells specific for self-antigen which can cause serious autoimmune conditions. (fredhutch.org)

Nude female BALB/c mice, 8- to 12-wk old, were injected intraperitoneally or intravenously. (snmjournals.org)
The r-BCG elicited a specific T cells response in Balb/c mice higher than wt BCG. (unicatt.it)
METHODS: Immunocompetent BALB/c mice were used to evaluate CRA's therapeutic effects in CRC. (bvsalud.org)

After these initial studies had yielded such promising results, Lukas Bunse expects that vaccine-induced T-cell receptors targeting brain tumor antigens could be a promising approach to develop new immunotherapies against glioblastoma. (dkfz.de)
Adaptive immune repertoires are composed by the ensemble of B and T-cell receptors within an individual, reflecting both past and current immune responses. (nature.com)
Olfactory receptors are supposed to act not only as molecular sensors for odorants but also as cell recognition molecules guiding the axons of olfactory neurons to their appropriate glomerulus in the olfactory bulb. (jneurosci.org)

Laboratory tests showed pancytopenia with severe thrombocytopenia (thrombocyte count 4.0 × 10 9 cells/L, erythrocyte count 3.34 × 10 12 cells/L, leukocyte count 2.92 × 10 9 cells/L). Neutropenia was also observed (neutrophil count 788 cells/mm 3 ), but hematuria was not observed. (cdc.gov)
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (exbio.cz)

Finally, to corroborate their results in humans, the authors identified HIV- and RSV-specific human B that expressed IgM, a type of BCR common in self-reactive B cells. (fredhutch.org)
Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Here, we report that CAR-T cells release extracellular vesicles, mostly in the form of exosomes that carry CAR on their surface. (nature.com)
Typically, CARs consist of a target binding domain, which is an extracellular domain that is specifically expressed by CAR-T cells, a transmembrane domain, and a signalling domain, which is an intracellular domain that provides an activation signal to T cells. (nature.com)
It is secreted by B cells and is found in blood and extracellular fluids. (anobase.org)
In order to overcome these limitations, this study explored the possibility to use cancer cell derived extracellular vesicles (EVs) as drug delivery system for oncolytic adenovirus. (helsinki.fi)
The extracellular domains of human and mouse EphB2 share 99% amino acid identity. (rndsystems.com)

CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (exbio.cz)
These glycoprotein complexes L-741626 play the crucial part in viral attachment, binding, fusion and access into the sponsor cell. (accessibletech4all.org)

intracellular antigen), amino acids 71-91. (abeomics.com)
Chlamydia organisms are obligate intracellular bacteria that must be isolated in tissue culture, mice, or chick embryos. (cdc.gov)

The rat PB gene promoter (454 bp) contains ARE [ 11 ] and directs a hormonal and developmental regulated expression, of a heterologous gene, specifically to the prostate in transgenic mice [ 12 ]. (hindawi.com)
Transgenic mice bearing PB promoter fused to simian virus 40 large tumor antigen gene all consistently develop prostate adenocarcinoma [ 13 ]. (hindawi.com)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

They fused "mouse myeloma cells secreting immunoglobulin of known specificity and human peripheral blood lymphocytes not secreting detectable immunoglobulin. (wikipedia.org)

The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
However, since only a few NLGN4X-specific T cells can be obtained in this way, which are not sufficient for cell therapy, the scientists resorted to a trick: they isolated the gene coding for the NLGN4X-specific T cell receptor. (dkfz.de)
1 Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children's Hospital and Houston Methodist Hospital. (aacrjournals.org)
Major histocompatibility complex products and endogenous retroviral gene products have both been shown to exert powerful influences on the frequency distribution of T-cell receptor β-chain variable region families in the mouse. (diabetesjournals.org)
The mechanisms of CRA on B cell differentiation were determined by cell-based analyses, including co-cultural with T cells, cytokine analysis, gene expression by qPCR, and protein expression by western blotting. (bvsalud.org)

Results: MICCop cells were able to inhibit the harmful autoreactive T-cell response and prevented mice from further relapses without affecting general immune responses. (uni-koeln.de)
Administered MICCop migrated to various organs leading to an increased infiltration of the spleen and the central nervous system with CD4(+)CD25(+)FoxP3(+) cells displaying a suppressive cytokine profile and inhibiting T-cell responses. (uni-koeln.de)
the genetic control of immune responses to T cell epitopes with respect to MHC and non-MHC linked genes. (imperial.ac.uk)
To "better understand B cell responses to vaccines," the Taylor lab in the Vaccine and Infectious Disease Division studied "the diverse populations of naïve B cells targeting antigens relevant for public health as well as model antigens that are more easily studied," Dr. Blair DeBuysscher explained. (fredhutch.org)

Methods: We replaced DCs by peripheral mononuclear cells and myelin autoantigens by glatiramer acetate (Copaxone (R)), a drug approved for the treatment of MS. Spleen cells were loaded with Copaxone (R), incubated with mitomycin C (MICCop) and injected into mice after the first bout of relapsing-remitting EAE. (uni-koeln.de)
Anti-Mouse CD90 (Thy-1) is present on thymocytes, most peripheral T-lymphocytes, some intraepithelial T-lymphocytes, epithelial cells, fibroblasts, neurons and hematopoietic stem cells, but not B-cells of most mice tested. (ichor.bio)

Previous work demonstrates that cross-reactive B cells are less responsive when exposed to their cognate foreign antigen. (fredhutch.org)
They transferred Nur77 eGFP -low, med, and high dye-labeled cells into recipients immunized with their cognate model antigen. (fredhutch.org)

As an evasion mechanism, many tumors are able to stimulate the expression of immune checkpoint molecules, resulting in an exhausted phenotype of T cells that cannot restrain tumor progression [ 1 ]. (oncotarget.com)
From 125I cell surface-labelled lysates TAL-1B5 immunoprecipitated a 33,000(alpha):28,000(beta) Ia dimer, but TAL-3C3 failed to immunoprecipitate cell surface molecules. (ox.ac.uk)
MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)

In order to assess the specificity of MICCop-induced suppression, treated EAE mice were challenged with the control protein ovalbumin. (uni-koeln.de)

CRA interacted with IL-6 receptor to activate STAT3 and cMaf, resulting in T cell secretion of IL-21, which, in turn induced B cell differentiation through the IL-21R/STAT3/Blimp-1 pathway. (bvsalud.org)
For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. (southernbiotech.com)
Hierarchical differentiation competence in response to retinoic acid ensures stem cell maintenance during mouse spermatogenesis. (southernbiotech.com)
Loss of IRF4 was detrimental to the expansion and differentiation of virus-specific NK cells. (medchemexpress.com)

Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)

Bunse's team then demonstrated that the NLGN4X-specific T cells are able to kill brain tumor cells in the culture dish. (dkfz.de)
Even though the idea of an immune system actively searching for and disposing of potential mutated tumor cells is over a century old, only recent developments in various fields such as mass spectrometry, immuno-checkpoint blockade strategies and in silico modelling have enabled the realization of the full potential of recruiting immune system to fight cancer and the possibilities of personalized therapies. (helsinki.fi)
This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)

The roles of antigen-specificity, responsiveness to TGFβ and APC subsets in tumour-induced expansion of regulatory T cells. (imperial.ac.uk)

Within this probabilistic framework, cell fate is specified along a linear developmental path that progresses from slowly proliferating long-lived to rapidly expanding short-lived subsets. (nih.gov)

Because of the strict varieties specificity of CMV illness, there is no animal model available for study of HCMV illness and immunity. (accessibletech4all.org)

Together, this dataset provides a resource for immunologists that can be integrated with future single-cell immune repertoire and transcriptome sequencing datasets. (nature.com)
In most mouse strains, these repertoire modifiers appear to be fully functional early in mouse development and shape a repertoire of antigen specificities that remains essentially unchanged from the first weeks of life until old age. (diabetesjournals.org)
Apart from purging the T cell repertoire from auto-reactive T cells via negative selection in the thymus dominant tolerance exerted by regulatory T cells plays a major role in tolerance imposition and maintenance. (epa.gov)

In this way, they succeeded in producing large numbers of T cells with identical specificity, all of which recognize the cancer antigen NLGN4X. (dkfz.de)

Unlike traditional mAbs, rAbs are generated using rabbit B cells, which have a higher affinity for binding to antigens. (uksfbooknews.net)
3. Collecting cell culture material for the ligandome affinity purification. (helsinki.fi)

4. Hodek M, Vávrová J, Šinkorová Z, Mokŕy J, Filip S. Hematopoietic recovery after transplantation CD117 + B220 - (LacZ + ) bone marrow cells in lethally irradiated mice. (southernbiotech.com)

Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. (southernbiotech.com)
Homing of lin - /CD117 + hematopoietic stem cells. (southernbiotech.com)

Recent advances in single-cell sequencing enable recovery of the complete adaptive immune receptor sequences in addition to transcriptional information. (nature.com)

In this study, we identified that the transcription factor IRF4 integrates signals to coordinate the NK cell response during mouse cytomegalovirus Infection . (medchemexpress.com)
Altogether, these data suggest that IRF4 is a signal integrator that acts as a secondary metabolic checkpoint to orchestrate the adaptive response of NK cells during viral Infection . (medchemexpress.com)
Investigators showed higher rates of infection in experimental mice deficient in the genes that control expression of these 2 agents. (medscape.com)

On serial sections through the olfactory bulb of mOR37-transgenic mouse lines, double-labeling experiments demonstrated that distinct immunoreactive glomeruli corresponded to glomeruli that were targeted by neurons expressing a particular member of the mOR37 receptor subfamily. (jneurosci.org)
These may be fimbrial or nonfimbrial, each with distinct receptor specificity. (medscape.com)

Zeiser R, Nguyen VH, Hou JZ, Beilhack A, Zambricki E, Buess M, Contag CH, Negrin RS: Early CD30 signaling is critical for adoptively transferred CD4+CD25+ regulatory T cells in prevention of acute graft-versus-host disease. (exbio.cz)
Among the various regulatory/suppressive cells hitherto described, CD4+CD25+ regulatory T cells (Treg) and interleukin-10 producing T regulatory 1 (Tr1) cells have been studied in most detail and are the subject of most articles in this issue. (epa.gov)

Both classic-pathway and alternate-pathway complement activation have been described, but the latter, which does not require the presence of immunoglobulins directed against bacterial antigens, appears to be the more active pathway in K pneumoniae infections. (medscape.com)
Lipopolysaccharide-binding protein facilitates transfer of bacterial cell wall components to inflammatory cells. (medscape.com)

C ) DOTATOC uptake by SSTR2-transduced and wild-type Jurkat T cells versus input DOTATOC concentration is shown. (jci.org)
In this study, we showed that after continuous oral consumption of high-fat (HF) diets containing M4N, the M4N concentration in most of the organs in mice reached ~1 µM (the M4N concentration in intestines and fat pads was as high as 20-40 µM) and treatment with the combination of M4N with temozolomide (TMZ) suppressed glycolysis and the tricarboxylic acid cycle in LN229 human glioblastoma implanted in xenograft mice. (bvsalud.org)

Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

Anatomy24

Organisms12

Diseases6

Chemicals and Drugs131

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes40

Disciplines and Occupations1

Information Science3

Health Care7

Antibodies27

Antibody41

Reactivity14

Chimeric antigen3

Target antigen2

Proliferation6

Sensitivity6

Cytotoxic2

Flow Cytometry5

IgG12

Recognizes2

Induced antigen retrieval1

Clone3

Vivo5

Assays2

Peptides3

Membrane8

Tissues8

Effector2

Assay3

Isotype Control1

ELISA1

Autoimmune4

BALB3

Receptors3

Leukocyte2

Humans5

Extracellular5

Glycoprotein2

Intracellular2

Transgenic mice3

Detectable1

Gene5

Responses4

Peripheral2

Cognate2

Molecules3

Suppression1

Differentiation4

CD452

Tumor cells3

Responsiveness1

Subsets1

Immunity1

Repertoire3

Recognize1

Affinity2

B2201

Hematopoietic stem2

Adaptive immune1

Infection3

Distinct2

REGULATORY T-CEL2

Bacterial2

Concentration2

Populations1

Induce1