Specificity antibodies to dengue neutralization tests. Medical search. Frequent questions

Last, because a dengue outbreak occurred in 2016, between the third and fourth serosurveys, we identified and compared demographic and clinical characteristics associated with DENV seroconversion in the outbreak and nonoutbreak periods. (cdc.gov)
Previously we and others reported key fusion loop (FL) residues and/or BC loop (BCL) residues on dengue virus (DENV) envelope protein recognized by flavivirus cross-reactive human monoclonal antibodies and polyclonal sera. (cdc.gov)
To improve ZIKV serodiagnosis, we employed wild type (WT) and FL or FL/BCL mutant virus-like particles (VLP) of ZIKV, DENV1 and West Nile virus (WNV) in enzyme linked immunosorbent assays (ELISA), and tested convalescent-phase serum or plasma samples from reverse-transcription PCR-confirmed cases with different ZIKV, DENV and WNV infections. (cdc.gov)
Dengue viral (DENV) infection results in a wide spectrum of clinical manifestations from asymptomatic, mild fever to severe hemorrhage diseases upon infection. (cdc.gov)
Laboratory testing with a rapid conducted at that time showed that 6.1% of the residents assay suggested that a dengue virus (DENV) was the caus- in nearby regions of Uganda had specifi c antibodies to ative agent. (folkhalsomyndigheten.se)
Some arboviruses, like dengue (DENV), chikungunya (CHIKV) and Zika (ZIKV), have become fully adapted to urban cycles and no longer require NHPs, forest mosquitoes and a sylvatic cycle for their maintenance [ 7 ]. (biomedcentral.com)
The four dengue serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) circulate throughout the Americas, and in some countries simultaneously. (paho.org)
While several lines of evidence suggest a protective role of T cells against disease associated with Dengue virus (DENV) infection, their potential contribution to immunopathology in the acute phase of DENV infection remains controversial, and it has been hypothesized that the more severe form of the disease (dengue hemorrhagic fever, DHF) is associated with altered T cell responses. (mdpi.com)
Dengue virus (DENV) is an enveloped, single-stranded RNA virus belonging to the Flaviviridae family. (frontiersin.org)
Developed by scientists at the New York State Department of Health Wadsworth Center and the University of Texas Medical Branch, the assay outperforms single antigen/antibody ZIKV IgM-Capture ELISA testing in overall sensitivity, specificity, positive predictive value and negative predictive value by detecting IgG-IgA-IgM antibodies to Zika Virus (ZIKV) and Dengue virus (DENV) serotypes 1-4. (healthresearch.org)

Despite this burden, data on dengue seroprevalence and force of infection (FOI), the rate at which initial or heterotypic infections are acquired, are scarce in Burkina Faso and Africa ( 7 ). (cdc.gov)
Diagnostic testing for Zika virus infection can be accomplished using both molecular and serologic methods. (cdc.gov)
Zika virus antibody testing is also complicated by cross-reactivity with other flaviviruses, which may make conclusive determination of which flavivirus is responsible for the person's recent infection difficult. (cdc.gov)
FDA has cleared one enzyme immunoassay to detect Zika virus IgM antibodies in serum and currently has issued Emergency Use Authorizations external icon for other IgM antibody assays to diagnose Zika virus infection. (cdc.gov)
During an infection only antibodies that bind to the pathogenic antigen with high affinity are produced. (wikipedia.org)
After a first encounter of the antigen by vaccination or natural infection, immunological memory allows for a more rapid production of neutralizing antibodies following the next exposure to the virus. (wikipedia.org)
Following infection with one serotype, subsequent infection with a different serotype increases a person's risk of severe dengue and death. (paho.org)
Dengvaxia is approved for use in individuals 9 through 16 years of age with laboratory-confirmed previous dengue infection and living in endemic areas. (cdc.gov)
Previous dengue infection can be assessed through (a) medical records of a previous laboratory-confirmed dengue infection or (b) serological testing prior to vaccination . (cdc.gov)
Dengue virus infection has emerged as marker in clinical practice ( 11 ). (who.int)
When the Zika epidemic spread through the American continent and then later to Africa and Asia in 2015, researchers compared the characteristics of the Zika infection to Dengue, considering both these viruses were transmitted primarily through the same vector, the Aedes aegypti female mosquitoes. (frontiersin.org)
Evidence suggesting an association of Zika virus infection with an increased risk for congenital microcephaly and other abnormalities of the brain and eye ( 5 ) prompted the World Health Organization to declare the Zika virus outbreak a Public Health Emergency of International Concern on February 1, 2016 ( http://www.who.int/mediacentre/news/statements/2016/1st-emergency-committee-zika/en/ external icon ). (cdc.gov)
Coxsackie B virus infection can be diagnosed by isolation of the virus in cell culture, detection of virus RNA via polymerase chain reaction (PCR) or serologic evaluation of viral antibodies. (medscape.com)
ELISA using an enteroviruses group-specific monoclonal antibody may detect early immunoglobulin M (IgM) antibody secreted during coxsackievirus B infection. (medscape.com)
B cells manufacture antibodies which are exquisitely tailored protein tools critical to controlling infection. (uvm.edu)
Alternatively, activated B cells can develop into memory B cells that persist long after infection to provide an institutional memory of those manufactured antibodies to facilitate their rapid remanufacture (or improvement) to protect against a subsequent pathogen encounter. (uvm.edu)
His goal is to develop novel immune metrics and specific antibody tools that can be used to advance vaccine development and to understand host-pathogen interactions during viral infection or vaccination. (uvm.edu)
Our work on dengue and zika immunology draws on an extensive bank of human vaccine clinical trial specimens and natural infection studies in partnership with the Vaccine Testing Center and other collaborators. (uvm.edu)
An extra step in our assay measures IgG antibodies by avidity testing, which determines the time-frame of infection, whether primary or secondary, and is quick and simple. (healthresearch.org)
The disease requires effective post-management of antibody production by complete healers and vaccinators because there is no perfect remedy for the virus infection. (kjcls.org)
Specificity, kinetics and longevity of antibody responses to avian influenza A(H7N9) virus infection in humans. (ox.ac.uk)
ObjectivesThe long-term dynamics of antibody responses in patients with influenza A(H7N9) virus infection are not well understood.MethodsWe conducted a longitudinal serological follow-up study in patients who were hospitalized with A(H7N9) virus infection, during 2013-2018. (ox.ac.uk)
HAI antibody responses were compared by clinical severity.ResultsOf 67 patients with A(H7N9) virus infection, HAI antibody titers reached 40 on average 11 days after illness onset and peaked at a titer of 290 after three months, and average titers of ≥80 and ≥40 were present until 11 months and 22 months respectively. (ox.ac.uk)
HAI antibody responses were significantly higher in patients who experienced severe disease, including respiratory failure and acute respiratory distress syndrome, compared with patients who experienced less severe illness.ConclusionsPatients with A(H7N9) virus infection who survived severe disease mounted higher antibody responses that persisted for longer periods compared with those that experienced moderate disease. (ox.ac.uk)
The test is done to screen patients for Hepatitis B infection. (frenovobio.com)
They should take further tests to determine if the Hepatitis B infection is acute or chronic. (frenovobio.com)

Aedes mosquitoes and dengue cases were documented in Africa as early as 1823, and cases have since been reported in 34 countries in Africa ( 2 ). (cdc.gov)
tory testing to the Arbovirus Diagnostic Laboratory at the Over the next 20 years, several ZIKV isolates were Centers for Disease Control and Prevention (CDC, Fort obtained from Aedes spp. (folkhalsomyndigheten.se)
In the Americas, Aedes aegypti is the mosquito vector that is the main source of dengue transmission. (paho.org)
125 countries includ- female mosquitoes, Aedes aegypti and Serum samples that tested positive ing Pakistan ( 2 , 3 ). (who.int)
Dengue is a disease transmitted by mosquitoes, with the primary vector being the Aedes Aegypti mosquito. (frontiersin.org)
Cultured Aedes albopictus cells (clone C6/36), persistently infected (PI) with dengue virus type 1 (dengue-1) were found resistant to superinfection with dengue virus type 3 (dengue-3). (microbiologyresearch.org)
Characteristics of Aedes albopictus cells persistently infected with dengue virus. (microbiologyresearch.org)
Repelente corporal baseado no Líquido da Casca da castanha de caju - LCC com ação repelente contra o mosquito Aedes aegypti transmissor da Dengue, Febre Chikungunya e Zika Mazzetto, Selma Elaine A fonte original deste documento pode ser restrita, o que pode impedir que leitores externos tenham acesso a ele. (paho.org)
Field trials and modelling studies suggest that elimination of dengue transmission may be possible through widespread release of Aedes aegypti mosquitoes infected with the insect bacterium Wolbachia pipientis (wMel strain), in conjunction with routine dengue control activities. (geneconvenevi.org)

By binding specifically to surface structures (antigen) on an infectious particle, neutralizing antibodies prevent the particle from interacting with its host cells it might infect and destroy. (wikipedia.org)
Neutralizing antibodies on the other hand can neutralize the biological effects of the antigen without a need for immune cells. (wikipedia.org)
When B cells are produced in the bone marrow, the genes that encode the antibodies undergo random genetic recombination (V(D)J recombination), which results in every mature B cell producing antibodies that differ in their amino acid sequence in the antigen-binding region. (wikipedia.org)
Plasma cells then secrete the antigen-specific antibody in large quantities. (wikipedia.org)
Serologic studies for viral antigen detection: Specific antibodies against enteroviral groups and serotypes are commercially available, but their application has thus far been limited to fluorescent staining and detection of viral antigens in cell cultures, rather than clinical specimens. (medscape.com)
This was determined by indirect immunofluorescent (IF) staining of cultures using monoclonal antibody against a dengue-3 type-specific antigen. (microbiologyresearch.org)
Dengue-1 PI cultures stained with this antibody 3 days after superinfection with dengue-3 virus (m.o.i. of 2) had dengue-3 antigen in 0.1 to 1.0% of the cells. (microbiologyresearch.org)
Control cultures infected with dengue-3 at the same multiplicity contained dengue-3 antigen in greater than 90% of the cells. (microbiologyresearch.org)
A small population of cells was also found (about 1%) that contained type-specific antigen of both dengue virus types. (microbiologyresearch.org)
Our serologic assay overcomes these barriers by using an antibody-antigen sandwich formed on six Luminex beads with ZIKV envelope, ZIKV NS1, DENV1 NS1, DENV2 NS1, DENV3 NS1 and DENV4 NS1 conjugated to polyvalent IgG+IgA+IgM detector antibodies. (healthresearch.org)
Natural human antibody responses to Plasmodium vivax apical membrane antigen 1 under low transmission and unstable malaria conditions in Sri Lanka. (ac.lk)
This allows me to test the exciting hypothesis that a non-HIV antigen may be required to elicit HIV-bnAbs. (wrfseattle.org)
The LINEAR Hepatitis B Antigen ( HBsAg ) Cassette Test detects HBSAG concentration greater than 2 ng/ml ( including any subtypes ) in human serum by the development of a colored line in the test region of the test device. (frenovobio.com)
The LINEAR Hepatitis B Antigen ( HBsAg ) Cassette Test uses an antibody that is highly specific for Hepatitis B Antigen ( HBsAg ) in serum. (frenovobio.com)
The Hepatitis B surface antigen (HBsAg) test is done to confirm the presence or absence of the Hepatitis virus in the blood. (frenovobio.com)

CDC is working with states to expand Zika virus laboratory testing capacity, using existing RT-PCR protocols. (cdc.gov)
This problem can be exacerbated when Zika virus testing is performed on patient populations not recommended in the Zika testing algorithms . (cdc.gov)
For this reason, Zika virus immunoglobulin (Ig) M antibody testing is recommended in certain situations . (cdc.gov)
While most state health departments and many commercial laboratories perform dengue and Zika virus NAAT and IgM antibody testing, confirmatory neutralizing antibody testing using PRNT is currently only available through a limited number of state health departments and CDC. (cdc.gov)
If Zika virus IgM antibody testing is positive and definitive diagnosis is needed for clinical or epidemiologic purposes, confirmatory PRNT should be performed against Zika and other flaviviruses endemic to the region where exposure occurred. (cdc.gov)
Consequently, in areas with high prevalence of dengue and Zika virus neutralizing antibodies, PRNT may not confirm a significant proportion of IgM positive results. (cdc.gov)
Therefore, such jurisdictions should make informed decisions about the utility of PRNT confirmation of IgM results depending on the prevalence of dengue and Zika virus neutralizing antibodies and observed performance of PRNT to confirm IgM test results. (cdc.gov)
The recent outbreaks of Zika virus (ZIKV) in flavivirus-endemic regions highlight the need for sensitive and specific serological tests. (cdc.gov)
It has been observed for Dengue virus and Zika virus. (wikipedia.org)
Updated guidelines include a new recommendation to offer serologic testing to asymptomatic pregnant women (women who do not report clinical illness consistent with Zika virus disease) who have traveled to areas with ongoing Zika virus transmission. (cdc.gov)
This update also expands guidance to women who reside in areas with ongoing Zika virus transmission, and includes recommendations for screening, testing, and management of pregnant women and recommendations for counseling women of reproductive age (15-44 years). (cdc.gov)
For pregnant women with clinical illness consistent with Zika virus disease,* testing is recommended during the first week of illness. (cdc.gov)
For asymptomatic pregnant women residing in areas with ongoing Zika virus transmission, testing is recommended at the initiation of prenatal care with follow-up testing mid-second trimester. (cdc.gov)
Local health officials should determine when to implement testing of asymptomatic pregnant women based on information about levels of Zika virus transmission and laboratory capacity. (cdc.gov)
All travelers to or residents of areas with ongoing Zika virus transmission should be advised to strictly follow steps to avoid mosquito bites because of the potential for exposure to Zika, dengue, and chikungunya viruses ( 6 ). (cdc.gov)
Dr. Diehl's work in the Department of Microbiology and Molecular Genetics encompasses translational and basic research aimed at understanding the human immune response to viral infections, primarily to dengue virus, zika virus, norovirus, and SARS-CoV-2. (uvm.edu)
A detailed comparison of the engagement of ZAb_FLEP antibody with the FLEP epitope on the Zika virus (ZIKV) based on a cryo-EM structure and EDE I C8 antibody with the EDE epitope was peer-reviewed and published in 2020 . (mit.edu)
2) Cross neutralization tests indicate that Zika virus is not related to yellow fever, Hawaii dengue nor to the FA and GD VII strains of Theiler's mouse encephalomyelitis virus. (paho.org)

However, they exhibit reduced specificity through cross-reactivity against other flaviviruses, e.g. dengue, circulating in South America. (ukri.org)
With IgM antibody testing, false-positive results are more common than with NAAT and can occur due to non-specific reactivity or cross-reactivity with other flaviviruses. (cdc.gov)
PRNT might not discriminate between flaviviruses antibodies, especially following secondary flavivirus infections. (cdc.gov)
The use of these cell lines for production of flaviviral antigens will expand serological diagnostic testing of flaviviruses worldwide. (cdc.gov)
Consideration of the genetic identity of both the Dengue and Zika flaviviruses as well as the cross-reactivity of relevant T cells along with the actions of CD4+ cytotoxic cells in these infections are also presented. (frontiersin.org)
The mosquito-borne flaviviruses dengue and zika cause endemic or pandemic disease and long-term sequelae across over 40% of the world's population and there are no broadly licensed vaccines for either. (uvm.edu)

It is transmitted by the bite of a mosquito infected with one of the four serotypes of dengue virus. (paho.org)
We determined the frequency of different serotypes of dengue virus to highlight its hyperendemicity in Rawalpindi, Pakistan. (who.int)

Testing is available through commercial laboratories, many state health departments, and the Centers for Disease Control and Prevention. (cdc.gov)
In 2008, PAHO / WHO Member States established a Network of Dengue Laboratories of the Americas (RELDA), to strengthen technical capacities for diagnosing dengue. (paho.org)
Human IgG antibody Laboratories manufactures the anti jc virus antibody tysabri reagents distributed by Genprice. (essentialbiosafety.info)
Human IgG antibody Laboratories manufactures the hepatitis a virus antibodies reagents distributed by Genprice. (essentialbiosafety.info)
Iga Antibody Laboratories manufactures the antibody neutralization igm iga igg reagents distributed by Genprice. (chipgrade.com)
Human IgG antibody Laboratories manufactures the nike zoom rival m 9 spikes reagents distributed by Genprice. (micrornaprofile.com)

Plaque reduction neutralization tests (PRNT) are quantitative assays that measure virus-specific neutralizing antibody titers. (cdc.gov)
RVPs display a quantitative and reproducible readout, enabling simple virus infectivity neutralization assays. (integralmolecular.com)
Fluorescent staining and neutralization assays: Using specific antibodies, these tests are used to further confirm and delineate the type of enterovirus isolated from cell culture. (medscape.com)
Molecular methods may soon replace viral culture and neutralization assays used to isolate and type enteroviruses. (medscape.com)
Viral culture and neutralization assays are reliable but time-consuming and costly methods. (medscape.com)
This is an important distinction since currently serological assays only use ZIKV envelope or ZIKV NS1 antigens, as well as only one antibody, usually IgM, for their diagnostic assays. (healthresearch.org)
A(H7N9) virus-specific antibody responses were assessed by hemagglutination inhibition (HAI) and neutralization (NT) assays. (ox.ac.uk)

For dengue, chikungunya, Zika and yellow fever viruses, sylvatic (forest) transmission cycles also exist in some regions and involve non-human primates and forest-dwelling mosquitoes. (biomedcentral.com)
Here we review the investigation methods and available data on sylvatic cycles involving non-human primates and dengue, chikungunya, Zika and yellow fever viruses in Africa, dengue viruses in Asia and yellow fever virus in the Americas. (biomedcentral.com)
Integral Molecular's seasonal Influenza RVPs represent specific strains for influenza A H1N1 and H3N2 and influenza B. Integral Molecular also offers a broad range of RVPs for other influenza strains including A H5N1 and H7N9, along with RVPs for an extensive selection of SARS-CoV-2 variants, and dengue, Zika, chikungunya, Ebola, and Nipah viruses. (integralmolecular.com)
These mosquitoes can also transmit dengue and chikungunya viruses ( 4 ). (cdc.gov)
To prevent human-to-mosquito-to-human transmission, persons infected with Zika, dengue, or chikungunya virus should protect themselves from mosquito exposure during the first week of illness. (cdc.gov)

For IgG ELISA, ZIKV WT-VLP had a sensitivity of 100% and specificity of 52.9%, which was improved to 83.3% by FL/BCL mutant VLP and 92.2% by the ratio of relative optical density of mutant to WT VLP. (cdc.gov)
RT-LAMP assay showed high sensitivity and specificity in isolating and typing coxsackieviruses. (medscape.com)

Annually, ≈50-100 million cases of dengue are reported worldwide, with 20,000 deaths ( 1 ). (cdc.gov)
50-100 million cases of dengue fever brate hosts of dengue virus in nature cessed by multiplex polymerase chain and 500 000 of dengue haemorrhagic are humans and several species of Asian reaction (PCR) in which the presence fever (DHF) reported annually ( 4 ). (who.int)

Dengue fever is a mosquitoborne disease caused by 4 related but antigenically distinct dengue viruses (DENVs), serotypes 1-4. (cdc.gov)
Diagnostic testing for these viruses is difficult because many of the pathogens require specialized biocontainment. (cdc.gov)
Neutralizing antibodies are part of the humoral response of the adaptive immune system against viruses, intracellular bacteria and microbial toxin. (wikipedia.org)
Viruses use a variety of mechanisms to evade neutralizing antibodies. (wikipedia.org)
Mutations that allow viruses to evade a neutralizing antibody will be selected for, and hence prevail. (wikipedia.org)
These RVPs offer researchers a ready-to-use reagent to reliably test vaccinee serum for influenza neutralization, providing an alternative to the standard assay method requiring laborious preparation of live viruses in cells or eggs. (integralmolecular.com)
With 20 years of experience focused on membrane proteins and antibodies, Integral Molecular's technologies have been integrated into the drug discovery pipelines of over 500 biotech and pharmaceutical companies to help discover new therapies for cancer, diabetes, autoimmune disorders, and viral threats such as SARS-CoV-2, Ebola, Zika, and dengue viruses. (integralmolecular.com)
Meanwhile, the cross-reactivity of CD8+ and CD4+ T cells response to Dengue and Zika viruses provide important clues for further development of potential treatments. (frontiersin.org)
Together, this information appraises the current understanding of both Zika and Dengue infections, providing insights for future vaccine design approaches against both viruses. (frontiersin.org)
Identification of dengue viruses from the Caribbean by plaque-reduction neutralization test. (microbiologyresearch.org)
More recently we seek to understand how norovirus, a family of viruses for which there is no vaccine and causes significant gastrointestinal illness (vomiting and diarrhea) and hospitalization can skew the infant antibody response towards those that may not offer protection. (uvm.edu)

IMPORTANCE Serological diagnostic testing for flaviviral infections is hindered by the need for specialized biocontainment for preparation of reagents and assay implementation. (cdc.gov)
With excellent reproducibility and ease of use, RVPs are commonly used as critical reagents for high-throughput testing of hundreds or thousands of samples in vaccine efficacy trials. (integralmolecular.com)

Dengue hemorrhagic fever - Do we know its cause? (microbiologyresearch.org)

a) ZIKV PRNT (most specific serological test for ZIKV). (ukri.org)
In March 2020 we implemented broad serological testing for SARS-CoV-2, the virus underlying COVID-19 used to profile hospitalized COVID-19 patients as well as those exposed to the virus but did not require hospitalization. (uvm.edu)

2015 we analysed the serum samples of 140 patients with a suspicion of dengue, using ELISA and multiplex polymerase chain reaction. (who.int)
Antibody has also been found in the serum of one of 15 wild monkeys tested. (paho.org)
The serum neutralization test confirmed that 70 of the 173 ELISA-positive sera had neutralizing antibodies against TBEV. (biomedcentral.com)
The Anti Jc Virus Antibody Tysabri reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (essentialbiosafety.info)
Description: Quantitativesandwich ELISA kit for measuring Pig Foot and Mouth Disease Virus (FMDV) Antibody (IgG) in samples from serum, plasma, cell culture supernates, tissue homogenates. (essentialbiosafety.info)
The HBsAg Rapid Test Cassette is a rapid test to qualitatively detect the presence of HBsAg in serum or plasma specimen. (frenovobio.com)
The test utilizes a combination of monoclonal and polyclonal antibodies to selectively detect elevated levels of HBsAg in serum or plasma. (frenovobio.com)
The Nike Zoom Rival M 9 Spikes reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (micrornaprofile.com)

ELISAs, IgM and IgG, detect antibody reponses to ZIKV over a longer time-frame. (ukri.org)
b) ZIKV NS1 ELISA (reduced cross-reactivity with other flaviviral antibodies). (ukri.org)
Tests and training will strengthen diagnostic services, and improve management of suspected ZIKV patients. (ukri.org)
This study clearly articulates the distinct engagement of ZAb_FLEP with the FLEP epitope that is different from the EDE I C8 antibody with the EDE epitope despite the overlap between these epitope regions on the surface of the ZIKV. (mit.edu)
Since the initial outbreak of the disease, it has been recognized that the sensitivities and specificities for current ZIKV testing algorithms have shortcomings. (healthresearch.org)
We tested a series of 2′-C- or 2′-O-methyl-substituted nucleosides, 2′-C-fluoro-2′-C-methyl-substituted nucleosides, 3′-O-methyl-substituted nucleosides, 3′-deoxynucleosides, derivatives with 4′-C-azido substitution, heterobase-modified nucleosides, and neplanocins for their ability to inhibit ZIKV replication in cell culture. (paho.org)
Antiviral activity was identified when 2′-C-methylated nucleosides were tested, suggesting that these compounds might represent promising lead candidates for further development of specific antivirals against ZIKV. (paho.org)

In May 2023, Oxitec voluntarily withdrew its research authorization application to test the use of genetically engineered mosquitoes in California. (geneconvenevi.org)

Monoclonal antibodies specific for dengue virus type 3. (microbiologyresearch.org)
Further work is aimed at identifying novel monoclonal antibodies and T cell responses to the virus. (uvm.edu)

Severe dengue is the leading cause of pediatric deaths and/or hospitalizations, which are a major public health burden in dengue-endemic or hyperendemic countries. (cdc.gov)
An important difference to note, however, was that the Zika epidemic diffused in a shorter time span compared to the persisting feature of Dengue infections, which is endemic in many Asian countries. (frontiersin.org)

These antigens can replace current antigens derived from live or inactivated virus for safer use in diagnostic testing. (cdc.gov)
Therefore, every B cell produces antibodies that bind specifically to different antigens. (wikipedia.org)
Immunological and biophysical separation of dengue-2 antigens. (microbiologyresearch.org)

This knowledge is critical to developing better vaccines and to develop better laboratory tests to determine whether vaccine candidates engage the immune system in a way that is likely to lead to protection. (uvm.edu)
1) Defining cellular immunogenicity for dengue vaccine formulations (Bill and Melinda Gates Foundation, NIH). (uvm.edu)
Vaccine recipients demonstrated strong antibody and T cell immune responses after 2 or 3 doses with 0.6 mg of INO-4700, a DNA vaccine that targets the MERS-CoV Spike (S) glycoprotein, delivered with intradermal CELLECTRA® device. (ivi.int)
Additionally, 92% of the vaccine recipients in both groups displayed the ability to neutralize the virus using a neutralization assay (EMC2012-Vero neutralization). (ivi.int)
2018. Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens. (bu.edu)
With this information, I hope to design a template for a vaccine that can teach our bodies to make HIV broadly neutralizing antibodies from scratch and protect us against HIV diversity. (wrfseattle.org)

The purpose of this assignment was to assess our selected article--Isolation of Potent SARS-CoV-2 Neutralizing Antibodies and Protection from Disease in a Small Animal Model--in terms of accuracy, availability, and reliability. (openwetware.org)
This assignment also served as a means for us to begin to look deeper at our selected article and learn more about SARS-CoV-2 neutralizing antibodies in small animal models. (openwetware.org)
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model. (openwetware.org)
SARS-CoV-2 mRNA vaccines induce greater complement activation and decreased viremia and Nef antibodies in men with HIV-1. (amedeo.com)
Description: SARS-CoV monoclonal Spike S1 VHH neutralizing antibody, a.a. 1-127(full length), with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. (chipgrade.com)
Description: Recombinant human monoclonal (clone 414-2) antibody recognizing the SARS-CoV-2 Spike S1 RBD glycoprotein. (chipgrade.com)
This antibody cross-reacts with the Spike protein from the SARS-CoV virus. (chipgrade.com)

Because of limited data on dengue virus in Burkina Faso, we conducted 4 consecutive age-stratified longitudinal serologic surveys, ≈6 months apart, among persons 1-55 years of age, during June 2015-March 2017, which included a 2016 outbreak. (cdc.gov)

Description: Neutralizing recombinant chimeric murine/human monoclonal antibody recognizing the extracellular domain of PD-L1 and blocking the binding of PD-L1 to PD-1. (chipgrade.com)

Test [PRNT] and Non-structural protein 1 [NS1] ELISA) and assess the time-frame postinfection that portable NAAT provide accurate diagnosis. (ukri.org)
Where research scientists come to find up to date resources on antibody discovery against challenging membrane protein targets. (integralmolecular.com)
Using sensitive high-throughput flow cytometry to identify binding interactions on a protein level, this technology has emerged as the leading solution for antibody specificity profiling. (integralmolecular.com)
Acknowledgments Supporting offer: DK077917 Abbreviations CYPcyclophosphamideNGFnerve development factorIGFinsulin-like development factorTGFtransforming development factorRPLPribosomal protein huge PStatsignal transducer and activator of transcriptionAPactivating proteins Footnotes Competing passions: the writers have no contending interests Author efforts: QLZ designed and executed a lot of the tests, and wrote area of the manuscript. (dietasrevisao.com)
Overall, for those receiving 0.6 mg of INO-4700, 88% demonstrated seroconversion after a 2 dose regimen at 0 and 8 weeks, while for those receiving a 3 dose regimen given at 0, 4 and 12 weeks, 84% seroverted after 2 doses and 100% after 3 doses, as measured by a binding antibody assay against the full-length S protein (ELISA). (ivi.int)
Systematic Bioinformatic Approach for Prediction of Linear B-Cell Epitopes on Dengue E and prM Protein.Advances in Bioinformatics, 2016, Article ID 1373157. (ac.lk)
P.D. Pushpakumara, P.H. Premarathne and C.L. Goonasekara (2016) Characterization of B cell epitopes of dengue virus NS1 protein using bioinformatics approach. (ac.lk)
Natural antibody responses to the capsid protein in sera of Dengue infected patients from Sri Lanka. (ac.lk)

Despite the specificity of molecular testing, false positive NAAT results have been reported in rare cases and may depend on the type of NAAT assay performed and patient population (i.e., limited or no prevalence of viral transmission) being tested. (cdc.gov)
As stated by Kuno & Chang [ 15 ], "The three commonly used data for identifying vertebrate reservoirs for arboviruses have been (i) virus isolation from suspected animals, (ii) relatively high antibody prevalence in the animals captured in the field and (iii) demonstration of viraemia (of higher virus titre and duration) in the suspected animals typically obtained under laboratory conditions" [ 15 ]. (biomedcentral.com)
The WNV RNA prevalence was 1.9% (95% CI, 1.4 to 2.9), while 14.3% (95% CI, 12.9 to 15.8) had WNV-neutralizing antibodies. (kjccm.org)

The specificity is not extremely high because the antibodies cross-react with other enteroviruses such as hepatitis A virus. (medscape.com)

Neutralizing antibodies can inhibit infectivity by binding to the pathogen and blocking the molecules needed for cell entry. (wikipedia.org)
Non-neutralizing antibodies, or binding antibodies, bind specifically to the pathogen, but do not interfere with their infectivity. (wikipedia.org)
Rapid titration of dengue virus type-4 infectivity by counting fluorescent foci. (microbiologyresearch.org)

A neutralizing antibody (NAb) is an antibody that defends a cell from a pathogen or infectious particle by neutralizing any effect it has biologically. (wikipedia.org)
Furthermore, neutralizing antibodies can act by preventing particles from undergoing structural changes often needed for successful cell entry. (wikipedia.org)
For example, neutralizing antibodies can prevent conformational changes of viral proteins that mediate the membrane fusion needed for entry into the host cell. (wikipedia.org)
Neutralizing antibodies are also important in neutralizing the toxic effects of bacterial toxins. (wikipedia.org)
An example of a neutralizing antibody is diphtheria antitoxin, which can neutralize the biological effects of diphtheria toxin. (wikipedia.org)
Neutralizing antibodies are not effective against extracellular bacteria, as the binding of antibodies does not prevent bacteria from replicating. (wikipedia.org)
Not all antibodies that bind to a pathogenic particle are neutralizing. (wikipedia.org)
Non-neutralizing antibodies can be important to flag the particle for immune cells, signaling that it has been targeted, after which the particle is processed and consequently destroyed by recruited immune cells. (wikipedia.org)
In some cases, non-neutralizing antibodies, or an insufficient amount of neutralizing antibodies binding to viral particles, can be utilized by some species of virus to facilitate uptake into their host cells. (wikipedia.org)
3) Defining B cell memory to Dengue and Zika and developing potent neutralizing antibodies (CDC and NIH, Collaboration with A. De Silva, University of North Carolina, Chapel Hill). (uvm.edu)
When we clogged NGF action with NGF neutralizing antibody in vivo, we found that the up-regulation of IGF-1 in the inflamed bladder was reversed as the up-regulation of TGF-1 had not been suffering from NGF neutralization. (dietasrevisao.com)
This study aimed to develop recombinant proteins specifically responsive to neutralizing antibodies in clinical specimens and use them to develop a rapid diagnostic kit to diagnose neutralizing antibodies quickly and conveniently against the COVID-19 virus and confirm the possibility of commercialization through a performance evaluation. (kjcls.org)
If the performance of the rapid diagnostic kit is improved and neutralizing antibodies can be analyzed quantitatively using quantitative analysis equipment, it can be used as important data to predict immunity to the COVID-19 virus and determine additional vaccinations. (kjcls.org)
In animals, the pooled seroprevalence of anti-WNV IgM and IgG was 90.3% (95% CI, 84.3 to 94.6) and 3.5% (95% CI, 1.9 to 5.8), respectively, while 20.0% (95% CI, 12.9 to 21.4) had WNV-neutralizing antibodies. (kjccm.org)
I study the developmental pathway of HIV broadly neutralizing antibodies (bnAbs) in longitudinal samples of super-infected individuals. (wrfseattle.org)
These super defenses are called broadly neutralizing antibodies. (wrfseattle.org)
Description: A Monoclonal antibody against Human NGF/proNGF Neutralizing. (chipgrade.com)
Description: Anti-NGF Neutralizing Recombinant Human antibody. (chipgrade.com)

The illness can evolve to severe dengue, characterized by shock, respiratory distress, severe bleeding, and/or serious organ impairment. (paho.org)
The disease can evolve into severe dengue, characterized by shock, shortness of breath, severe bleeding and / or complications in the organs. (paho.org)

is the vector mosquito for dengue and is widely distributed in the Americas. (paho.org)
Only Canada and continental Chile are free from dengue and its vector. (paho.org)
There is currently no specific treatment for dengue, and vector control and vaccination are the only effective methods to prevent future outbreaks. (who.int)
aegypti) is the major vector that transmits many diseases including dengue, Zika, and filariasis in tropical and subtropical regions. (geneconvenevi.org)

6) Of 99 human sera tested, 6 (6.1 per cent. (paho.org)
A total of 4114 individual goat sera were screened for TBEV-specific antibodies using immunological methods. (biomedcentral.com)

The 2016 outbreak included 1,061 dengue rapid-diagnostic test (RDT) positive cases and 15 deaths in the capital, Ouagadougou, with a reported case-fatality rate (CFR) of 1.2% ( 4 , 6 ). (cdc.gov)
Tests, protocols and training will be available to local laboratory networks for patient use and to support further diagnostic studies. (ukri.org)
I built tools to study this virus and contributed to a diagnostic test during the first year of my M.Sc. (wrfseattle.org)
What is the HBsAg test kit for Diagnostic? (frenovobio.com)

Nucleic acid amplification test, or NAAT, is a generic term referring to all molecular tests used to detect viral genomic material. (cdc.gov)
The combined approach of screening vertebrate hosts for TBEV-specific antibodies followed by testing the local tick population for TBEV allowed us to detect two new TBEV foci in the canton of Valais. (biomedcentral.com)

This antibody has been shown to cross-react with mouse PD-L1. (chipgrade.com)
This antibody does not cross-react with mouse PD-1, but does cross-react with monkey (M. fascicularis) PD-1. (chipgrade.com)

Finally, a summary of the immune biomarkers that have been reported for dengue and Zika viral infections are discussed which may be useful indicators for future anti-viral targets or predictors for disease severity. (frontiersin.org)

Integral Molecular has developed the Membrane Proteome Array (MPA) platform to de-risk antibody-based therapeutics by testing specificity across the full human membrane proteome expressed in live cells. (integralmolecular.com)
With a focus on specificity screening, this webinar assembles experts in antibody discovery and development and features presentations from Integral Molecular, Cabaletta Bio, and The University of Pennsylvania. (integralmolecular.com)
5) Cellular and molecular characterization of dengue-associated rash (Merck). (uvm.edu)
A coalition representing over 120,000 medical and laboratory professionals and institutions that perform the vast majority of clinical molecular pathology testing in the United States released a joint statement regarding new Medicare prices for those tests. (blogspot.com)
The coalition's concerns include cuts in reimbursement for molecular pathology tests, denials of claims without publication of sufficient information on the basis for those decisions, and incorrect determinations that certain tests are investigational. (blogspot.com)

Despite revised clinical classifications of dengue infections by the World Health Organization, the wide spectrum of the manifestations of dengue illness continues to pose challenges in diagnosis and patient management for clinicians. (frontiersin.org)
A result of 99.5 % concordance to the ELISA test was determined by a clinical study of 1208 samples. (frenovobio.com)

Other Antibody products are available in stock. (chipgrade.com)

Currently available diagnostics: nucleic acid amplification tests (NAAT) and antibody ELISA, have critical problems [1]. (ukri.org)

ABSTRACT Dengue is one of the most important arthropod-borne viral diseases. (who.int)
Dengue virus is transmitted to was approved by the Ethics Committee borne viral diseases worldwide ( 1 ). (who.int)
In October 1990, in collaboration with CSTE, CDC published Case Definitions for Public Health Surveillance (4), which, for the first time, provided uniform criteria for reporting cases to increase the specificity of reporting and improve the comparability of diseases reported from different geographic areas. (cdc.gov)

This review discusses similarities between Dengue and Zika infections, comparing their disease transmissions and vectors involved, and both the innate and adaptive immune responses in these infections. (frontiersin.org)
A random intercept model was used to fit a curve to HAI antibody responses over time. (ox.ac.uk)

this population is not likely to qualify for access to confirmatory plaque reduction neutralization testing (PRNT) and therefore the virus could go undetected. (healthresearch.org)

Mahesha N. Nadugala, Chandima Jeewandara, Ramesh S. Jadi, Gathsaurie N. Malavige, Aravinda M. de Silva, Prasad H. Premaratne and Charitha L. Goonasekara (2021) Natural immunogenic properties of bioinformatically predicted linear B‑cell epitopes of dengue envelope and pre‑membrane proteins. (ac.lk)

Application of Next-Generation Sequencing to Reveal How Evolutionary Dynamics of Viral Population Shape Dengue Epidemiology. (cdc.gov)

Dengue incidence has increased in the Americas over the past four decades, from 1.5 million cumulative cases in the 1980s to 16,2 million in the decade 2010-2019. (paho.org)
Incidence of dengue has increased many fold in the past 50 years and has expanded to areas that were previously free from the disease. (who.int)

Be the first to learn more about exclusive virology antibody products, services and latest updates. (creative-biolabs.com)

In terms of seroprevalence in Burkina Faso, 1 study found the dengue IgG seroprevalence among 683 pregnant women and blood donors to be 26.3% in rural and 36.5% in urban settings in 2003-2004 ( 8 ). (cdc.gov)

Anatomy12

Organisms57

Diseases39

Chemicals and Drugs68

Analytical, Diagnostic and Therapeutic Techniques and Equipment47

Phenomena and Processes31

Disciplines and Occupations1

Information Science4

Named Groups1

Health Care9

Geographicals3

DENV10

Infection26

Aedes9

Antigen15

Zika Virus18

Flaviviruses6

Serotypes of dengue virus2

Laboratories6

Assays7

Chikungunya5

Sensitivity2

Million cases of dengue2

Viruses11

Reagents2

Hemorrhagic fever1

Serological2

Serum8

ZIKV7

Mosquitoes1

Monoclonal antibodies2

Endemic2

Antigens3

Vaccine6

SARS-CoV7

Serologic1

Human monoclonal1

Protein8

Prevalence3

Hepatitis A vir1

Infectivity3

Neutralizing18

Severe2

Vector4

Sera2

Diagnostic4

Detect2

Cross-react2

Viral infections1

Molecular5

Clinical2

Products are available in stock1

NAAT1

Diseases3

Responses2

Confirmatory1

Membrane proteins1

Epidemiology1

Incidence2

Virology1

Seroprevalence1