Specificity and human mice clone cells. Medical search. Frequent questions

To eliminate auto-immune reactivity, several levels of checkpoints throughout B cell development prevent self-reactive B cells from developing or becoming activated. (fredhutch.org)
However, this system can also backfire, as some B cells specific for a foreign antigen may also display low-level cross-reactivity for self-antigen, leading to their deletion or hindrance. (fredhutch.org)
Because these B cells have never encountered the antigen for which they are specific, any Nur77 eGFP expression, and therefore BCR stimulation, can be attributed to cross-reactivity to a self-antigen. (fredhutch.org)
The degree of cross-reactivity with self-antigen varied between B cells specific for various model antigens, but was not increased specifically in model antigen-specific B cells compared to polyclonal B cells of unknown specificities. (fredhutch.org)
Because model antigens are convenient tools that may not be representative of antigens derived from human pathogens, the authors next repeated this experiment with naïve B cells specific for HIV, respiratory syncytial virus (RSV) and influenza virus, and again found the same level of cross-reactivity to self-antigen. (fredhutch.org)
Together, these experiments showed that a range of cross-reactivity to self is observed within B cells of a given specificity, but that cross reactivity to self is not limited to B cells specific for foreign antigens. (fredhutch.org)
To determine if this lack of responsiveness corresponded to a reduced ability to proliferate after BCR stimulation, Nur77 eGFP -low, medium, and high B cells were identified as a proxy for increasing levels of cross-reactivity with self-antigen and incubated with anti-immunoglobulin to stimulate the BCR. (fredhutch.org)
Based on these in vitro results, the authors moved to a mouse model to determine if cells displaying high self-reactivity would have dampened antigen responses in vivo . (fredhutch.org)
They found that Nur77 eGFP -low and high cells proliferated least compared to Nur77 eGFP -medium donor cells, suggesting that self-reactivity acts as a secondary signal to tune B cells responses to antigen and that a moderate level of self-reactivity corresponds with optimal B cell function. (fredhutch.org)
Moderate self-antigen reactivity corresponds with optimal B cell activity against foreign antigens. (fredhutch.org)
As in mouse cells, human B cells specific for foreign antigens did not express differential amounts of IgM compared to bulk B cells, suggesting that cross-reactivity to self is a B cell-wide trait not limited to B cells of certain specificities. (fredhutch.org)
Species Reactivity: Human. (scytek.com)
Analysis of the binding of these antibodies to either human TFPI K3 or full-length TFPIα revealed high affinity binding (6H7: Kd = 0.3nM (K3), Kd = 0.7nM (TFPI)), (4D7: Kd = 0.6nM (K3), Kd = 1.3nM (TFPI)), and no cross-reactivity with murine TFPI. (isth.org)
NKp30 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. (prospecbio.com)

The observation that most autoantibodies in traditionally autoantibody-mediated diseases are of the IgG isotype and carry somatic mutations strongly suggests T-cell help in the autoimmune B-cell response. (hindawi.com)
This item is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. (biocheminfo.org)
Mouse T1/ST2 (IL-33 R) PE conjugated monoclonal antibody (Clone DJ8, Host / Isotype Subclass: Rat IgG1, light chain not isotyped) for the identification and purification of murine T helper 2 (Th2). (mdbioproducts.com)

The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes. (rndsystems.com)
When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescent staining prior to acquisition on a flow cytometer. (bdbiosciences.com)
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. (bdbiosciences.com)
Binding of the Fc portion of the antibodies to FcR leads to activation of effector cells and further release of proinflammatory cytokines and proteolytic enzymes, mediators of antibody-dependent cell-mediated cytotoxicity (ADCC). (hindawi.com)
c) Effect of antibodies and antigen-specific B cells on antigen uptake. (hindawi.com)
B cells, the immune cells that produce antibodies, each recognize and bind a specific antigen through their B cell receptor (BCR). (fredhutch.org)
The lack of binding of mIgG to hFcRn explains why murine antibodies are rapidly removed from the circulation in humans, despite long half-life in mice 32 . (nature.com)
We currently offer a TotalSeq™-A Human Universal Cocktail v1.0 that contains 163 antibodies, and our scientists have utilized 200+ markers for internal and custom applications. (mediray.co.nz)
When cells stained by these antibodies are lysed, the mRNA is captured by the bead and the ADT is also captured in an identical fashion. (mediray.co.nz)
Since the development of CITE-seq and REAP-seq, TotalSeq™ antibodies have been used in an expanding number of applications and with new single-cell platforms. (mediray.co.nz)
To generate and characterise anti-human TFPI K3 monoclonal antibodies (mAbs) that inhibit/do not inhibit TFPI-protein S cofactor function. (isth.org)
Following selection of clones with immunoreactivity against human TFPI K3, two antibodies were selected for full characterisation (6H7 & 4D7). (isth.org)
These data confirm the importance of the TFPI K3 domain in protein S-dependent enhancement of TFPI function and reveal that distinct epitopes on the K3 are important for the interaction with protein S. These antibodies will pave the way for interrogation of protein S-TFPI cofactor function in human plasma. (isth.org)
Second, a study undertaken by the Pasteur Institute of Paris in collaboration with the EMVT (Département Elevage et Médecine Vétérinaire Tropicale du Centre de Coopération Internationale pour la Recherche en Agronomie pour le Développement) showed that 4% of sheep bred in Chad and Ethiopia had anti-RVFV neutralizing antibodies ( 4 ). (cdc.gov)
Yet immunization of humans with recombinant Env leads to the production of antibodies with very narrow breadth of neutralization which fail to block infection of diverse circulating viral isolates. (washington.edu)
Expanded pools of diverse B cell clones compete for antigen and T cell help in a Darwinian process that ultimately selects for B cells with higher affinity receptors which can be secreted as soluble molecules (antibodies). (washington.edu)
As an alternative to using HIV-1 Env as a vaccine immunogen, we are pursuing a novel vaccine strategy where we generate anti-idiotypic antibodies: antibodies raised in a non-human animal model that bind with high affinity and specificity to bNAb precursors. (washington.edu)
One of these antibodies, R69-4, protected against cartilage antibody- and collagen-induced arthritis in mice, but not autoimmune disease models independent of arthritogenic autoantibodies. (bvsalud.org)
The epitope specificity of the antibodies in relapsing polychondritis differs from those in RA, suggesting different mechanisms for formation and pathophysiologic roles. (medscape.com)

Immunogen: Proteins from human Leiomyoma. (scytek.com)
Immunogen: SDS extract of human myocardium. (calpaclab.com)

Mouse IgG1 heavy chain and κ light chain. (prospecbio.com)

Hybridoma clones have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human IL-1, β. (advimmuno.com)
Anti-human NKp30 mAb, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c immunized with recombinant human NKp30 amino acids 19-138 purified from E. coli. (prospecbio.com)

Hybridoma clones were screened and selected based on mAb affinity, specificity, and inhibitory/non-inhibitory function on the protein S enhancement of TFPI-mediated FXa inhibition. (isth.org)
It is produced in hybridoma cells and purified by affinity chromatography. (invivogen.com)
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human recombinant protein of Carboxypeptidase A2 (CPA2). (angioproteomie.com)

In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. (rndsystems.com)
In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. (rndsystems.com)
It reacts with ACTH-producing cells (corticotrophs). (teomics.com)
The monoclonal antibody K041 (CY-90) reacts highly specifically with human cytokeratin18 in a variety of simple epithelia (e.g. with intestinal, bronchial, urinary tract, liver and glandular epithelia of prostate and uterus). (histoprime.com)
Specificity: This antibody reacts with actin of skeletal, cardiac, and smooth muscle cells. (calpaclab.com)

Likewise, antigen/antibody complexes are bound by the FcR of APCs and processed in a unique fashion dependent on the epitope specificity of the bound antibody. (hindawi.com)

The objective of this study is to investigate its biochemical and structural characteristics and substrate specificity in order to provide insight regarding its involvement in these diseases. (rhea-db.org)
Molecular dynamics simulations using the crystal structures of Trypanosoma cruzi TAT and human TAT provided further insight regarding the substrate-enzyme interactions and substrate specificity. (rhea-db.org)
Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. (thermofisher.com)

2000). "Prediction of the coding sequences of unidentified human genes. (wikipedia.org)
Full-length cDNA sequences for both mouse and human polycystin-L2 were obtained from testis cDNA. (nih.gov)
Likewise, the authors directly cloned HIV-specific BCR sequences and assessed binding to a human cell line, but found that HIV-specific B cells were no more self-reactive than naïve, polyclonal B cells. (fredhutch.org)
The Committee concluded that full-length genome sequences from additional variola major and minor strains, particularly Congo 70 and Somalia 77, should be determined and that additional clone libraries from selected strains should be prepared. (who.int)

Genes within the BCR undergo random recombination during development, giving rise to B cell repertoires with immense diversity. (fredhutch.org)
In the work by Qian et al, conversion of mouse astrocytes to neurons was demonstrated by lentiviral-mediated shRNA knockdown (KD) of Ptbp1 (shPTB) resulting in neuronal morphology and expression of neuronal markers TUJ1 and MAP2 concomitant with suppression of astrocyte-specific genes. (vectorbuilder.jp)
Naive BCRs arise through the random recombination of germline-encoded immunoglobulin genes during B cell development. (washington.edu)
Recently, two N-acetyltransferase genes, Nat-1 and Nat-2, were cloned from rapid (C57BL/6J) and slow (A/J) acetylator mouse strains. (cdc.gov)
In this report, the N-acetylation polymorphism in mice was investigated by transiently expressing the cloned N-acetyltransferase genes in COS-1 cells. (cdc.gov)

Specificity: Highly expressed in peripheral blood mononuclear cells. (scytek.com)
The original antibody was generated by isolating an scFv from an immunized human antibody library created from the peripheral blood mononuclear cells of six SARS convalescent patients. (absoluteantibody.com)

This antibody recognizes the LAT-adaptor protein expressed on T-cells, mast cells and megacaryocytes. (inserm-transfert.com)
It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K10). (progen.com)
The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q. (origene.com)

PD-L1 CAR haNKs reduced levels of macrophages and other myeloid cells endogenously expressing high PD-L1 in peripheral blood from patients with head and neck cancer. (elifesciences.org)
Vβ11 is expressed by 0.4% to 0.9% of peripheral CD3+ cells in normal blood. (beckman.com)
By using C6/36 and Vero cell lines, peripheral blood lymphocytes collected from two soldiers on duty in Chad during the 2001 rainy season were cocultured. (cdc.gov)

Due to the conflicting reports published, use of monoclonal MAB4227 may result in an underestimation of CXCR7 expression on certain cell types. (rndsystems.com)
Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. (rndsystems.com)
In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). (rndsystems.com)
From these mice, B cells specific for a "model" antigen and assessed for Nur77 eGFP expression. (fredhutch.org)
Western Blot analysis of CESK1 expression in transfected 293T cell line by CESK1 monoclonal antibody (M01), clone 4G6. (abnova.com)
The expression cassettes of the gRNAs are tandemly ligated into a single vector using the Golden Gate cloning method. (nature.com)
Two recent papers from Qian et al (4) and Zhou et al (5) demonstrate that reduced expression of the RNA binding protein PTB (encoded by Ptbp1-polypyrimidine tract-binding protein) allows the direct conversion of glial cells to neurons. (vectorbuilder.jp)
Allogeneic-donor-derived cells can be genetically modified to eliminate expression of HLA-A. (ashpublications.org)
Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)
Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis. (nih.gov)
Given that MHCI molecules are potent regulators of immune responses, we postulated that the expression of MHCI by tumor cells influences systemic immune responses. (bvsalud.org)
Our study highlights a critical role of NK cells beyond its identity as a killer lymphocyte and more importantly, the potential host responses to a localized tumor as determined by its MHCI expression. (bvsalud.org)

2 IgE binds to high-affinity receptors on tissue mast cells and circulating basophils. (respiratory-therapy.com)
Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. (calpaclab.com)
Studies in mouse indicate the 80 aa SMAF1 protein is involved in adipocyte tissue function or regulation. (nih.gov)
E4 showed a binding pattern restricted to skin, macrophages and dendritic cells in lymphoid tissue, and cartilage derived from mouse and human arthritic joints. (bvsalud.org)
Spleen and kidney tissue of wild-type mice (Ref.1). (origene.com)
Spleen and kidney tissue of C1q -/- mice (Ref.1). (origene.com)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

Mouse TAT (mTAT) was cloned from a mouse cDNA library, and its recombinant protein was produced using Escherichia coli cells and purified using various chromatographic techniques. (rhea-db.org)

We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. (nature.com)
These vaccine candidates retain all previously characterized attenuation phenotypes of the PDK-53 vaccine virus, including attenuation of neurovirulence for 1-day-old CD-1 mice, absence of virulence in interferon receptor-deficient mice, and lack of transmissibility in the main mosquito vectors. (cdc.gov)
In this thesis, human glia-to-neuron direct conversion and engineered viral vectors are explored using pre-clinical in vitro and ex vivo models. (lu.se)

Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. (elifesciences.org)
We adapted the human whole-blood model to murine blood. (frontiersin.org)
Unlike human blood, murine blood was unable to reduce fungal burden and more substantial filamentation of C. albicans was observed. (frontiersin.org)
Our results reveal substantial differences between murine and human whole-blood models infected with C. albicans and thereby demonstrate limitations in the translatability of this ex vivo model between hosts. (frontiersin.org)
Both human and murine polycystin-L2 proteins are predicted to have seven putative transmembrane (TM) domains, and, by comparison with transient receptor potential channels, the six carboxyl-terminal TM domains are likely to constitute an ion channel subunit. (nih.gov)
To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 (aka TLR11) and TLR13 in mice, the homolog of TLR10 being a pseudogene. (invivogen.com)

To "better understand B cell responses to vaccines," the Taylor lab in the Vaccine and Infectious Disease Division studied "the diverse populations of naïve B cells targeting antigens relevant for public health as well as model antigens that are more easily studied," Dr. Blair DeBuysscher explained. (fredhutch.org)
An invariant Vα24/Vβ11 T cell receptor is expressed in all individuals by clonal expanded CD4- CD8- T cells, reactive to bacterial antigens. (beckman.com)
As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. (origene.com)
T-cell receptors (TCR) and B-cell receptors (BCR) are hypervariable immunoglobulins that play a key role in recognizing antigens in the vertebrate immune system. (elifesciences.org)
The evidence for an autoimmune etiology includes pathological findings of infiltrating T cells, the presence of antigen-antibody complexes in affected cartilage, cellular and humoral responses against collagen type II and other collagen antigens, and the observation that immunosuppressive regimens most often suppress the disease. (medscape.com)

After overnight incubation, secreted embryonic alkaline phosphatase (SEAP) activity in the cell culture supernatant was assessed using QUANTI-Blue™ Solution . (invivogen.com)
The antigen used was a precipitate (polyethylene glycol 6000) of the supernatant of Vero cells infected with the RVFV clone 13. (cdc.gov)

Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. (calpaclab.com)
Group 2 innate lymphoid cells protect lung endothelial cells from pyroptosis in sepsis. (mdbioproducts.com)

Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene. (wikipedia.org)
This protein is a subunit of the cleavage and polyadenylation specificity factor (CPSF) complex which plays a key role in pre-mRNA 3' end processing and polyadenylation. (wikipedia.org)
Confocal immunofluorescent analysis of COS cells, mock-transfected (left) or transfected with HA-tagged protein (right), using HA-Tag (6E2) Mouse mAb (green). (cellsignal.com)
Besides histones, HDAC6 deacetylates also other substrates including alpha tubulin and HSP90 alpha, and is involved in protein trafficking and degradation, as well as in affecting of cell shape and migration. (abeomics.com)
Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), developed by the New York Genome Center (NYGC), brings together surface protein phenotyping (similar to flow cytometry) and single-cell RNA sequencing (scRNA-seq) 1 . (mediray.co.nz)
Since Cas9 has no DNA recognition specificity except for the PAM sequence, simple multiplication of gRNAs along with a common Cas9 protein results in multiplex genome engineering. (nature.com)
NKp30 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. (prospecbio.com)
The isolation of monoclonal bNAbs from HIV-1+ individuals has provided valuable information on how bNAbs develop during infection, and on the epitope specificities on the HIV-1 Envelope protein (Env) that an effective vaccine should aim to elicit. (washington.edu)
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

The role of B cells in autoimmune diseases involves different cellular functions, including the well-established secretion of autoantibodies, autoantigen presentation and ensuing reciprocal interactions with T cells, secretion of inflammatory cytokines, and the generation of ectopic germinal centers. (hindawi.com)
Through these mechanisms B cells are involved both in autoimmune diseases that are traditionally viewed as antibody mediated and also in autoimmune diseases that are commonly classified as T cell mediated. (hindawi.com)
This new understanding of the role of B cells opened up novel therapeutic options for the treatment of autoimmune diseases. (hindawi.com)
Traditionally, autoimmune disorders were classified as T cell mediated or autoantibody mediated. (hindawi.com)
Likewise B cells function as crucial antigen presenting cells in autoimmune diseases that are traditionally viewed as T cell mediated. (hindawi.com)
however, it needs to be emphasized that most autoimmune diseases are driven by a dysfunction in the immune network consisting of B cells, T cells, and other immune cells. (hindawi.com)
However, the stochastic nature of B cell development also produces B cells specific for self-antigen which can cause serious autoimmune conditions. (fredhutch.org)
The specificity of autoimmune injury to cartilaginous tissues has led investigators to test the hypothesis that a cartilage-specific autoantibody is central to the pathogenesis of relapsing polychondritis. (medscape.com)

T cell-based immunotherapy, such as immune checkpoint blockade or adoptive T cell transfer, is limited by the ability of T cells to detect major histocompatibility complex (MHC)-presented antigen by tumor cells. (elifesciences.org)
NKp30 is a natural cytotoxicity receptor (NCR) that may assist NK cells in the lysis of tumor cells. (prospecbio.com)
Downregulation of MHC class I (MHCI) molecules on tumor cells is recognized as a resistance mechanism of cancer immunotherapy. (bvsalud.org)
Accordingly, mice-bearing MHCI-deficient tumor cells showed reduced tumor-associated extramedullary myelopoiesis in the spleen. (bvsalud.org)

Right panel: antigen binds to the BCR of antigen-specific B cells and is internalized. (hindawi.com)

In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. (rndsystems.com)
Sequence analysis predicts that the mouse and human polycystin-L2 proteins consist of 621 and 624 amino acid residues, respectively. (nih.gov)
The cell barcode is used to associate the sequence to a specific cell and a unique molecular identifier (UMI) distinguishes the specific mRNA molecule. (mediray.co.nz)
For each marker to be uniquely identified, each antibody clone has a unique barcode added to the poly(A) sequence. (mediray.co.nz)

First, ELISA screening was done by using antigen capture (by mouse hyperimmune ascitic fluid) and detecting specific IgG in the diluted serum (1/500). (cdc.gov)
Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH. (sigmaaldrich.com)
ELISA test results were confirmed by using lyssavirus virus in newborn mice (9) from the brains of 24 bats that rapid fluorescent focus inhibition test (RFFIT) on 144 gave uncertain IFA results were unsuccessful. (cdc.gov)

Detects human CXCR7/RDC‑1 in direct ELISAs. (rndsystems.com)
Detects human Integrin alpha 6/CD49f. (rndsystems.com)

A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. (ox.ac.uk)
Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. (ox.ac.uk)

Likewise, human isotypes of R69-4 protected against arthritis with comparable efficiency. (bvsalud.org)

To determine to what degree naïve B cells specific for foreign antigen were cross-reactive to self-antigen, the authors used Nur77 eGFP reporter mice whose B cells fluoresce following BCR stimulation in a signal strength-dependent manner. (fredhutch.org)
Previous work demonstrates that cross-reactive B cells are less responsive when exposed to their cognate foreign antigen. (fredhutch.org)
Finally, to corroborate their results in humans, the authors identified HIV- and RSV-specific human B that expressed IgM, a type of BCR common in self-reactive B cells. (fredhutch.org)
It is not reactive with other mesenchymal cells except for myoepithelium. (calpaclab.com)
The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. (progen.com)

The MAbs recognize the highly conserved HA1 region of H5N1 hemagglutinin and inhibit multiple strains of the H5N1 virus, as well as treated mice infected with a lethal dose of H5N1 viruses of two divergent strains, demonstrating their potential as therapeutic agents for multivalent prophylaxis and treatment of influenza. (justia.com)

Whole-blood models can help to elucidate host-pathogens interactions and have been used for several Candida species in human blood. (frontiersin.org)
Species: MouseImmunogen: SDS extract of human myocardium. (calpaclab.com)
Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. (biocheminfo.org)

Molecular Cell. (wikipedia.org)

The key inflammatory cells involved in the genesis of asthma are mast cells, eosinophils, lymphocytes, and epithelial cells. (respiratory-therapy.com)
Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. (mdbioproducts.com)

In HERA, rescue from degradation depends on FcRn, and engineered ligands are recycled in a manner that correlates with their half-lives in human FcRn transgenic mice. (nature.com)

Left panel: antigen bound by antibody is taken up via FcR on APCs such as dendritic cells or macrophages. (hindawi.com)
C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. (origene.com)

Epithelial cells and their derivatives typically consist of intermediate filaments composed of approximately 20 different related polypeptides. (histoprime.com)
Tumors originating from epithelia retain the cytokeratin expressions of normal epithelial cells. (histoprime.com)
It does not react with squamous epithelia of the following organs: epidermis, hair follicles, sebaceous glands, tongue, esophagus and vagina or with non-epithelial cells. (histoprime.com)
aegypti ingest blood from a viremic human and the virus infects midgut epithelial cells. (cdc.gov)

Traditionally, cell cultures, such as VERO, have been used for virus production in bovine serum-containing culture media. (cdc.gov)

NK cells express germ-line receptors that are either stimulatory or inhibitory, and the summation of these signals determines activation status ( Caligiuri, 2008 ). (elifesciences.org)
Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)

The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. (origene.com)

Furthermore, both the mouse and human receptors bind more strongly to mouse serum albumin (MSA) than to HSA. (nature.com)

All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. (cellsignal.com)

Natural killer (NK) cell-based immunotherapy may overcome genetic mechanisms of resistance to T cell-based immunotherapy through antigen- and MHC-independent recognition of malignant cells. (elifesciences.org)
This unique lymphocyte population restricted by the CD1d molecule recognition has been identified as the natural killer T (NKT) cells. (beckman.com)
Involvement of leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of human Toll-like receptor 2 in the recognition of diacylated lipoproteins and lipopeptides and Staphylococcus aureus peptidoglycans. (invivogen.com)
Specifically targeting certain somatic cells circumvents immune recognition and bypasses the need to revert back to an embryonic state thus avoiding the loss of essential age-related and epigenetic factors. (vectorbuilder.jp)
One of the first critical steps in an effective antibody response is the recognition of a foreign antigen by a membrane anchored B cell receptor (BCR) on the surface of a naïve B cell. (washington.edu)

Failed T cell-based immunotherapies in the presence of genomic alterations in antigen presentations pathways may be overcome by NK cell-based immunotherapy. (elifesciences.org)
The genomic clone encoding NAT-1 is identical in rapid and slow acetylator mouse strains, whereas the clone encoding NAT-2 differs between rapid and slow strains by a single base pair, which changes the encoded amino acid from Asn99 in the rapid acetylator strain to Ile99 in the slow acetylator strain. (cdc.gov)

HepG2 cells. (teomics.com)
RT-PCR analysis showed that the full-length transcript is expressed in human brain, kidney, testis, and HepG2 cells, and there are three alternatively spliced variants that were differentially expressed. (nih.gov)

Independent of antibody secretion B cells secrete proinflammatory cytokines, support the formation of ectopic GCs, and serve as antigen presenting cells. (hindawi.com)
These anaphylatoxins promote release of proinflammatory cytokines and serve as chemoattractants for effector cells. (hindawi.com)
Upon exposure to an initiating stimulus (an asthma inducer), these inflammatory cells release inflammatory mediators such as histamine, leukotrienes, prostaglandins, cytokines, chemokines, and platelet-activating factor. (respiratory-therapy.com)

Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)
PTB and it's neuronal analogue nPTB are both down regulated during neurogenesis (6) and in vitro knockdown of Ptbp1 is sufficient for conversion of mouse fibroblasts to neurons (7). (vectorbuilder.jp)
Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
Influence of organochlorine pesticides on development of mouse embryos in vitro . (cdc.gov)

Mice were immunised with recombinant human TFPI K3 expressed in insect cells. (isth.org)

Western blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using HA-Tag (6E2) Mouse mAb. (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom). (cellsignal.com)
Immunocytochemical staining of COS cells without transfection (left), expressing HA-tagged Akt3 (middle) or expressing HA-tagged Estrogen Receptor (right), using HA-Tag (6E2) Mouse mAb. (cellsignal.com)
Vβ11 is the human variable β11 chain of the T cell receptor. (beckman.com)
Anti-hTLR6-IgG (clone C5C8) is a monoclonal antibody specific for human Toll-like receptor 6 (hTLR6, CD286). (invivogen.com)
NKp30 interacts with CD3-zeta (CD247), a T-cell receptor. (prospecbio.com)

It is possible to convert one somatic cell type to another with the best-known example being the reprogramming of fibroblasts to induced pluripotent stem (iPS) cells by "Yamanaka factors" (1). (vectorbuilder.jp)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

marker for chick cells in chick/quail chimeras. (uiowa.edu)
To demonstrate in vivo reprogramming of astrocytes, AAV expressing shPTB (together with a loxP-Stop-LoxP(LSL)-RFP) were injected into the substantia nigra of wild type and transgeneic mice expressing Cre driven by the astrocyte-specific marker GFAB. (vectorbuilder.jp)

Mice have a similar genetic polymorphism in N-acetyltransferase activity and have been used as models of the human polymorphism in many studies of the toxicology and carcinogenicity of arylamines. (cdc.gov)
Based on previous experience in using the well-characterized and clinically proven dengue virus serotype-2 (DENV-2) PDK-53 vaccine backbone for live-attenuated chimeric flavivirus vaccine development, we developed chimeric DENV-2/ZIKV vaccine candidates optimized for growth and genetic stability in Vero cells. (cdc.gov)

PKD2L2 was mapped to human chromosome 5q31 and Pkd2l2 to mouse chromosome 18 in band C. (nih.gov)

Reciprocal roles of T-cell help for B cells during adaptive immune responses and B-cell help in CD4+ T-cell activation are being increasingly recognized. (hindawi.com)
Btn2a2 Regulates ILC2-T Cell Cross Talk in Type 2 Immune Responses. (mdbioproducts.com)

Western blot analysis of CESK1 over-expressed 293 cell line, cotransfected with CESK1 Validated Chimera RNAi ( Cat # H00150160-R01V ) (Lane 2) or non-transfected control (Lane 1). (abnova.com)

Anatomy23

Organisms19

Chemicals and Drugs76

Analytical, Diagnostic and Therapeutic Techniques and Equipment58

Phenomena and Processes88

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Information Science6

Health Care8

Reactivity14

Isotype3

Antibodies20

Immunogen2

IgG11

Immunized with recombinant human2

Hybridoma3

Reacts5

Epitope specificity1

Substrate3

Sequences4

Genes5

Mononuclear cells2

Recognizes3

Peripheral3

Expression12

Tissue8

Recombinant protein1

Vectors3

Murine6

Antigens5

Supernatant2

Endothelial cells2

Protein9

Autoimmune8

Tumor cells4

Binds1

Sequence4

ELISA3

Detects2

Myeloma2

Likewise1

Reactive5

MAbs1

Species3

Molecular1

Mast cells4

Transgenic mice1

Dendritic cells2

Epithelial cells4

Bovine1

Inhibitory2

C57BL1

Receptors1

Formulation1

Recognition5

Genomic2

HepG22

Cytokines3

Vitro4

Insect Cells1

Receptor6

Somatic2

Marker2

Genetic2

Chromosome1

Immune responses2

RNAi1