• a1) T cells can also interact with, and be activated by, DCs presenting via MHC/HLA a specific antigen matching the T-cell receptor, and accompanied by co-stimulatory (CD80/CD86 DCs, and CD28 T cells) and adhesive molecules (ICAM-1/LFA-3 DCs, and LFA-1/CD2 T cells). (weizmann.ac.il)
  • Although the nature of the antigens responsible for RA pathogenesis is unknown, there is evidence that the disease-associated HLA-DR (B1*0401, 0404, 0405 and 0101) molecules are involved in disease pathogenesis [ 2 ]. (biomedcentral.com)
  • Several environmental, genetic, and immune factors create a "perfect storm" for the development of coeliac disease: the antigen gluten, the strong association of coeliac disease with HLA, the deamidation of gluten peptides by the enzyme transglutaminase 2 (TG2) generating peptides that bind strongly to the predisposing HLA-DQ2 or HLA-DQ8 molecules, and the ensuing unrestrained T cell response. (frontiersin.org)
  • In this review, we will discuss how tissue destruction in the context of coeliac disease results from the complex interactions between gluten, HLA molecules, TG2, and multiple innate and adaptive immune components. (frontiersin.org)
  • However, while up to 40% of the general population in Western countries express one of the predisposing HLA molecules, the global prevalence of CeD is just 1% ( 8 ). (frontiersin.org)
  • MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. (exbio.cz)
  • Immune-dominant regions containing multiple epitopes in silico predicted to bind to HLA class II molecules were found for four of the five structural proteins, while no such regions were predicted for HLA class I. Experimentally, CHIKV-specific CD4+ T-cells were detected in six out of twelve participants after a single MV-CHIK vaccination and more robust responses were found 4 weeks after two vaccinations (ten out of twelve participants). (bvsalud.org)
  • Like conventional antigens/haptens, superantigens stimulate antigen-presenting dendritic cells by increasing the expression of HLA-DR antigen and co-stimulatory molecules (CD54, CD83, and CD86) as well as the generation of tumour necrosis factor (TNF) -α. (microbiologynote.com)
  • In spite of interacting with MHC class II molecules outside the antigen-binding groove, they are nevertheless able to induce a fruitful contact with T cells. (microbiologynote.com)
  • HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). (thermofisher.com)
  • Adoptive cell therapy using engineered T-cell receptors (TCRs) targeting cancer-testis antigens, such as Melanoma-associated antigen 3 (MAGE-A3), is a potential approach for the treatment of NSCLC. (thno.org)
  • However, systematic analysis of T cell immune responses to MAGE-A3 antigen and corresponding antigen-specific TCR is still lacking. (thno.org)
  • In this study, we comprehensively screened HLA-A2 restricted MAGE-A3 tumor epitopes and characterized the corresponding TCRs using in vitro artificial antigen presentation cells (APC) system, single-cell transcriptome and TCR V(D)J sequencing, and machine-learning. (thno.org)
  • We identified the HLA-A2 restricted T cell epitopes from MAGE-A3 that could effectively induce the activation and cytotoxicity of CD8+ T cells using artificial APC in vitro . (thno.org)
  • A cohort of HLA-A2+ NSCLC donors demonstrated that the number of epitope specific CD8+ T cells increased in NSCLC than healthy controls when measured with tetramer derived from the candidate MAGE-A3 epitopes, especially epitope Mp4 (MAGE-A3: 160-169, LVFGIELMEV). (thno.org)
  • Statistical and machine-learning based analyses demonstrated that the MAGE-A3-Mp4 epitope-specific CD8+ T cell clones were mostly in effector and proliferating state. (thno.org)
  • Importantly, T cells artificially expressing the MAGE-A3-Mp4 specific TCRs exhibited strong MAGE-A3+ tumor cell recognition and killing effect. (thno.org)
  • Cross-reactivity risk analysis of the candidates TCRs showed high binding stability to MAGE-A3-Mp4 epitope and low risk of cross-reaction. (thno.org)
  • This work identified candidate TCRs potentially suitable for TCR-T design targeting HLA-A2 restricted MAGE-A3 tumor antigen. (thno.org)
  • The humanized MAGE\A3 TCR (SRm1g13t)\transfected T\cells showed enhanced cytotoxicity. (fsu93.org)
  • c) The gel filtration chromatography of refolded peptide human being leucocyte antigen (pHLA) (MAGE\A3) and biotinylation effectiveness analysis of purified pHLA (MAGE\A3). (fsu93.org)
  • Tests for antibodies to liver-kidney microsome type 1 (anti-LKM-1), soluble liver antigen (anti-SLA), and liver cytosol type 1 (anti-LC1) may be helpful if antinuclear antibody (ANA) and anti-smooth muscle antibody (ASMA) test results are negative. (medscape.com)
  • Carbohydrate xenoantigens and xenoantibody epitope-specificity, the removal of xenoantibodies by plasmapheresis, and antibody neutralization using soluble saccharides, were all explored. (gu.se)
  • One of these vaccines is based on a measles virus vector encoding for the CHIKV structural genes C, E3, E2, 6K, and E1 (MV-CHIK), which proved safe in phase I and II clinical trials and elicited CHIKV-specific antibody responses in adult measles seropositive vaccine recipients. (bvsalud.org)
  • Lessons learned from the study of NK cell memory could help with the design of better expansion protocols for adoptive NK cell therapy, for the manufacturing of chimeric antigen receptor (CAR)-engineered NK cells and for improving NK cell-based therapies that rely on antibody-dependent cellular cytotoxicity (ADCC). (nature.com)
  • This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • The following product was used in this experiment: HLA-DR Monoclonal Antibody (LN3), Super Bright™ 436, eBioscience™ from Thermo Fisher Scientific, catalog # 62-9956-42, RRID AB_2744822. (thermofisher.com)
  • The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (thermofisher.com)
  • HIV-1-Specific Chimeric Antigen Receptors Based on Broadly Neutralizing Antibodies. (ucsf.edu)
  • First, the major histocompatibility complex (MHC) and T-cell receptor (TCR) contact residues were identified by analysis of single site substituted analogue peptides for HLA-DR4 binding and cognate T cell recognition using both T hybridomas and polyclonal T cells from peptide-immunized HLA-DR4 transgenic mice. (biomedcentral.com)
  • In the large-scale benchmark calculation consisting of 216 known HIV epitopes covering all 12 recognized HLA supertypes, the NetCTL-1.2 method was shown to have a sensitivity among the 5% top-scoring peptides above 0.72. (biomedcentral.com)
  • The most restrictive step involved in antigen presentation is binding of the peptide to MHC class I. It is estimated that only 1 out of 200 peptides will bind a given MHC class I allele with sufficient strength to elicit a CTL response [ 9 ]. (biomedcentral.com)
  • These peptides were then tested in our previously described oncolytic cancer vaccine platform PeptiCRAd, a vaccine platform that combines an immunogenic oncolytic adenovirus (OAd) coated with tumor antigen peptides. (elifesciences.org)
  • HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. (thermofisher.com)
  • Analysis by sequential immunoprecipitations of the specificities of the monoclonal antibodies TU22,34,35,36,37,39,43,58 and YD1/63.HLK directed against human HLA class II antigens. (anticorps-enligne.fr)
  • however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
  • Clones from three patients with a partial response or stable disease recognized antigens expressed on renal cell carcinoma tumor cells. (aacrjournals.org)
  • CD8 + CTL-recognizing minor H antigens on tumor cells can be isolated posttransplant and could contribute to the graft- versus -tumor effect. (aacrjournals.org)
  • Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. (exbio.cz)
  • The latter is a tool previously developed by Jacopo, 2020, able to identify tumor antigens similar to pathogen antigens in order to exploit molecular mimicry and tumor pathogen cross-reactive T cells in cancer vaccine development. (elifesciences.org)
  • T-cells were mainly directed against the three large structural proteins C, E2 and E1. (bvsalud.org)
  • The human leukocyte antigen ( HLA ) is a gene complex that encodes the major histocompatibility complex ( MHC ) proteins . (amboss.com)
  • RÉSUMÉ Nous avons étudié l'efficacité de la campagne de vaccination antirougeoleuse de masse à Orumieh (République islamique d'Iran) en examinant la séroprévalence des IgG anti-rougeole et la réponse en anticorps à partir de données appariées avant et après la campagne. (who.int)
  • Introduction : les valeurs de référence peuvent être obtenues à partir des bases de données. (bvsalud.org)
  • Nous nous proposons de produire ces valeurs à partir d'une base de données hématologiques et de souligner les contraintes inhérentes à cette méthodologie. (bvsalud.org)
  • We sought to identify an altered peptide ligand (APL) based on the endogenously expressed synovial auto-epitope of human cartilage glycoprotein-39 (HC gp-39) for modulation of cognate, HLA-DR4-restricted T cells. (biomedcentral.com)
  • CeD is triggered by gluten consumption in genetically susceptible individuals carrying certain major histocompatibility complex (MHC) class II human leukocyte antigen (HLA) variants ( 5 , 6 ). (frontiersin.org)
  • During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
  • Overall primary concerns are: extrapolating human clinical studies, seeding durable effective T cell resident memory (Trm), population human leucocyte antigen (HLA) coverage, and the potential for T cell-mediated immune escape. (mdpi.com)
  • Figure B. PE conjugated Mouse anti Human CD11c ( MCA2087PE ) and Amethyst Orange conjugated Mouse anti Human HLA DP DQ DR ( MCA477AMO ). (bio-rad-antibodies.com)
  • As an example, cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) have been detected in the serum of ovarian cancer patients. (biomedcentral.com)
  • Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. (thermofisher.com)
  • Second, NK cells can remember inflammatory cytokine milieus that imprint long-lasting non-antigen-specific NK cell effector function. (nature.com)
  • The hybridomas all respond to the HC gp-39(263-275) epitope when bound to HLA-DR4(B1*0401) but differ in their fine specificities. (biomedcentral.com)
  • The vast number of activated T-cells produces a massive immunological response that is not specific to any one epitope on the SAg, so undermining one of the key advantages of the adaptive immune system, namely its capacity to target antigens with great specificity. (microbiologynote.com)
  • Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. (thermofisher.com)
  • In fact, the HLA locus which is the main inherited genetic susceptibility factor for CeD, only accounts for ~ 40% of the genetic variance of the disease. (frontiersin.org)
  • Patients were monitored for donor engraftment of myeloid and lymphoid cells, for clinical response by serial imaging, and for immunologic response by in vitro isolation of donor-derived CD8 + CTLs recognizing recipient minor histocompatibility (H) antigens. (aacrjournals.org)
  • a4) Activated T-cells form intercellular contacts with each other and cluster through ICAM-1-LFA-1 binding. (weizmann.ac.il)
  • T cell immunity is at the center of the disease contributing to the inflammatory process through the loss of tolerance to gluten and the differentiation of HLA-DQ2 or HLA-DQ8-restricted anti-gluten inflammatory CD4 + T cells secreting pro-inflammatory cytokines and to the killing of intestinal epithelial cells by cytotoxic intraepithelial CD8 + lymphocytes. (frontiersin.org)
  • Airway mucosal T cells induced by infection have a distinct hierarchy of antigen specificity compared to the periphery. (bvsalud.org)
  • Distinct receptor-ligand interactions and distinct cytokine milieus lead to the generation of antigen-specific memory NK cells. (nature.com)
  • Cytokine-induced memory NK cells can be generated by exposure to inflammatory cytokines even in the absence of a defined antigen. (nature.com)
  • First, similarly to T cells and B cells, NK cells can exert immunological memory after encounters with stimuli such as haptens or viruses, resulting in the generation of antigen-specific memory NK cells. (nature.com)
  • Activated B cells and memory T cells can recognize specific antigens on pathogens. (amboss.com)
  • In a normal antigen-induced T-cell response, 0.0001-0.001% of the body's T-cells are activated, but these SAgs can activate up to 20% of the body's T-cells. (microbiologynote.com)
  • Once secreted, superantigens engage with antigen-presenting cells without further processing. (microbiologynote.com)
  • EBV miRNAs also inhibit the expression of viral antigens, thereby enabling infected cells to escape immune recognition. (ijbs.com)
  • HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. (thermofisher.com)
  • It also binds the abnormally folded HLA-B27 homodimer found in spondylarthritides, but not the normal heterodimer of HLA-B27 with beta 2‑microglobulin (9, 10). (rndsystems.com)
  • Modulation of Single-Cell Gene Expression and Cell Function in Evolving Manufacturing Processes for Clinical Trials With Enhanced-Affinity T-Cell Receptor T-Cell Therapy Targeting the MAGE-A4 Antigen in Solid Tumors, American Society of Cell and Gene Therapy, 2023, Davis, S., et al. (adaptimmune.com)
  • This finding suggests that these HLA variants contribute to, but are not sufficient for, the development of the disease and that additional genetic and environmental factors are needed to mount a pathogenic immune response against gluten ( 9 ). (frontiersin.org)
  • Nous avons procédé à une étude rétrospective des dossiers médicaux de 28 garçons et 22 filles (âge moyen lors de la survenue 10,5 ± 2,6 ans), chez lesquels le diagnostic avait été confirmé sur la base des critères de Jones révisés, à l'Institut cardiologique de la reine Alia de février 1999 à février 2002. (who.int)
  • Multiple immunological abnormalities have been reported in T1D patients including autoantibody production against the insulin molecule, the 65 kD isoform of glutamic acid decarboxylase (GAD65), various islet antigens, and the zinc transporter 8 (ZnT8) as well as decreased regulatory T cell (Treg) capacity to suppress T-cell mediated destruction of the islets of Langerhans 3 . (nature.com)
  • Highly polymorphic regions (such as HLA genes) are not represented either due to difficulty with designing primers because of the lack of constant regions flanking the variants. (tripod.com)
  • The affinity of humanized TCR was optimized further by phage display after transforming the TCR to a solitary\chain TCR variable\fragment (sTv).34, 35 Our study suggests that the CDR grafting strategy utilized for TCR humanization can enable the humanized TCR to retain the specificity and affinity of the parent murine TCR. (fsu93.org)
  • The innate immune system develops in utero and, unlike the adaptive (acquired) immune system , does not require imprinting or adaptation to specific antigens nor does it provide permanent pathogen -specific immunity . (amboss.com)
  • Intriguingly, EBV miRNAs directly suppress host antiviral immunity by interfering with antigen presentation and immune cell activation. (ijbs.com)
  • This enhancement by superantigens is suppressible by both corticosteroids and cyclosporine, but typical antigen/hapten-induced enhancement is resistant to cyclosporine suppression. (microbiologynote.com)
  • The term "superantigen" was used by Kappler and Marrack in 1989 to describe this fundamentally new class of antigens. (microbiologynote.com)
  • This phase I trial assessed the safety, efficacy, and immunologic responses to minor histocompatibility antigens following nonmyeloablative allogeneic hematopoietic cell transplantation as treatment for metastatic renal cell carcinoma. (aacrjournals.org)
  • Eight patients received conditioning with fludarabine and low-dose total body irradiation followed by hematopoietic cell transplantation from an HLA-matched sibling donor. (aacrjournals.org)
  • In the synthetic niche, interactions with the functionalized scaffold are expected to provide the signals needed for T-cell activation (with or without antigen specificity), lineage selection, proliferation, and survival. (weizmann.ac.il)
  • For this purpose we employed a panel of well-characterized T cell hybridomas generated from HC gp-39-immunized HLA-DR4 transgenic mice. (biomedcentral.com)
  • The amino acids Ala268, Ser269, Glu271 and Thr272 constituted the major TCR contact residues, as substitution at these positions did not affect HLA-DR4(B1*0401) binding but abrogated T cell responses. (biomedcentral.com)
  • Autologous T cell therapy for MAGE-A4+ solid cancers in HLA-A*02+ patients: a phase 1 trial. (adaptimmune.com)
  • As for GAD65 antigen (GAD-alum) treatment per se in new onset T1D, an initial 2008 report of 70 patients was auspicious insofar as residual beta-cell function over 30 months was somewhat preserved. (nature.com)
  • Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. (thermofisher.com)
  • the specificity Foeex a T cell does not define its functional capa- bilities. (allforexindicators.com)
  • When these APLs were analysed, a partial TCR agonist was identified and found to modulate the HC gp-39(263-275)-specific, pro-inflammatory response in HLA-DR4 transgenic mice. (biomedcentral.com)
  • The latter suggests the ongoing of an antigen-driven response [ 1 ]. (biomedcentral.com)
  • CD8 + CTL clones recognizing minor H antigens were isolated from five patients studied. (aacrjournals.org)
  • 90-95% of CeD patients carry the HLA-DQ2.5 variant (DQA1*05:01, DQB1*02:01) that confers the highest risk of developing CeD while the remaining patients carry HLA-DQ2.2 (DQA1*02:01, DQB1*02:02) or HLA-DQ8 (DQA1*03, DQB1*03:02) ( 5 , 7 ). (frontiersin.org)
  • Méthodes : il s'agissait d'une étude prospective réalisée sur une population de 482 patients, consultés pour suspicion du paludisme au Centre Hospitalier Universitaire de la Mère et de l'Enfant Lagune (CHU-MEL) au Bénin, dans la période de Mai à Août 2015. (bvsalud.org)
  • Conclusion : l'utilisation du coefficient 6000 dans l'estimation de la parasitémie, a entraîné une sous-estimation significative de la charge parasitaire et n'était pas adaptée à la réalité des patients consultés au CHU-MEL. (bvsalud.org)
  • Blood group phenotypes relating to expression of ABO antigens in individual kidneys were identified. (gu.se)
  • Based on A antigen expression, a hypothesis explaining why blood group A2 kidneys could be successfully transplanted to O recipients was postulated. (gu.se)
  • Memory response becomes more potent and faster after subsequent exposures to an antigen . (amboss.com)
  • Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. (thermofisher.com)