Two recent papers from Qian et al (4) and Zhou et al (5) demonstrate that reduced expression of the RNA binding protein PTB (encoded by Ptbp1-polypyrimidine tract-binding protein) allows the direct conversion of glial cells to neurons. (vectorbuilder.jp)
The researchers showed that as the cell senses low oxygen conditions, HIF-1 α begins regulating the RNA splicing factor, Polypyrimidine tract-binding protein 1 (Ptbp1), that in turn directs the splicing of the CEACAM1 gene, leading to the protective CEACAM1-S version that reduces the liver injury that accompanies transplantation. (technologynetworks.com)
Splice site1
It induces female-specific alternative splicing of the transformer (tra) pre-mRNA by binding to the tra uridine-rich polypyrimidine tract at the non-sex-specific 3' splice site during the sex-determination process. (wikipedia.org)
Uridine-rich2
SXL contains an N-terminal Gly/Asn-rich domain that may be responsible for the protein-protein interaction, and tandem RNA recognition motifs (RRMs) that show high preference to bind single-stranded, uridine-rich target RNA transcripts. (wikipedia.org)
This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
RBPs6
RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
RNA-binding proteins (RBPs) regulate gene expression at the post-transcriptional level. (encyclopedia.pub)
LIN28 (LIN28A and LIN28B) proteins are highly conserved small cytoplasmic RNA-binding proteins (RBPs) that function as pluripotency factors, regulating the transition from self-renewal to a differentiated cell fate [ 1 ] . (encyclopedia.pub)
Cytoplasmic1
ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)
MRNA9
The IRES directing the translation of p53 is in the 5'-untranslated region of the mRNA, whereas the IRES mediating the translation of ΔN-p53 extends further into the protein-coding region. (iisc.ac.in)
Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
This machinery recognizes pre-mRNA sequences at several motifs - the 5′ and 3′ splice sites, the branch point, and polypyrimidine tracts - positioned at exon-intron boundaries [ 4 ]. (biomedcentral.com)
Phosphorylation by mTOR of 4E-BP1 disrupts its binding to eIF4E, a protein that binds the 5'-cap structure of mRNA. (biomedcentral.com)
Its pre-mRNA is sex-specifically spliced and encodes female-specific or male-specific polypeptides. (bioone.org)
Bmdsx pre-mRNA transcribed derived from transgene was sex-specifically spliced. (bioone.org)
Attenuation2
The mechanism of attenuation of angiogenic proteins by ibuprofen was further investigated. (akjournals.com)
By means of oligonucleotide microarray and RNA interference, we reveal that the sensitizing effect of IFN-β was possibly due to attenuation of MGMT expression via induction of the protein p53. (aacrjournals.org)
Isoforms1
They play major roles in the tissue- and stage-specific expression of protein isoforms as well as in the maintenance of protein homeostasis. (encyclopedia.pub)
Gene3
In fruit flies, this protein participates in alternative splicing of the transformer gene, deciding the sex of the fly. (wikipedia.org)
Sex-lethal, the master sex-determining gene in Drosophila, is not sex-specifically regulated in Musca domestica" (PDF). (wikipedia.org)
As this page just shows up to 10 assays to the query, if you wish to have the complete list of assays that the query gene/probe was specifically expressed, click here . (or.jp)
Cellular4
While these agents target physiological processes such as angiogenesis and DNA repair, targeting cellular structures that form specifically in malignant cells is another approach to tackle cancer. (biomedcentral.com)
Specifically, the researchers found that Hypoxia Inducible Factor 1 (HIF-1α), which regulates oxygen consumption, played a central role in orchestrating the activation of the version of CEACAM1, called CEACAM1-S, that limits cellular injury and improves liver function in mice. (technologynetworks.com)
The DNA repair protein O 6 -methylguanine-DNA methyltransferase (MGMT) plays an important role in cellular resistance to alkylating agents. (aacrjournals.org)
A cellular DNA-repair protein, namely O 6 -methylguanine-DNA methyltransferase (MGMT) protein, reverses alkylation at the O 6 position of guanine, thereby inhibiting the lethal cross-linking and bringing about resistance to alkylating agents ( 2, 3 ). (aacrjournals.org)
Mechanism1
The probable mechanism of action of PTB protein has also been investigated. (iisc.ac.in)
Specificity2
Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
Genes2
The transcriptomic and epigenomic regulatory landscape of the MAC genome, which has 80% protein-coding genes and short intergenic regions, is poorly understood. (uni-frankfurt.de)
Knockout of two 2-oxoglutarate-dependent dioxygenases (2OGD) genes from the candidate region converted black seeds to yellow seeds, suggesting that these two 2OGD proteins catalyze the conversion of yellow metabolites to black metabolites. (bvsalud.org)
Synthesis2
One of the important mechanisms used for protein synthesis during these conditions is internal initiation. (iisc.ac.in)
In many cell lines, exposure to rapamycin results in a relatively small decrease in overall protein synthesis (~15-20%), but dose result specifically in G1 cell cycle arrest. (biomedcentral.com)
Pathway1
This pathway, called alternative splicing, is an adaptation that cells use to boost their protein diversity in times of danger, inflammation, and injury. (technologynetworks.com)
Macrocyclic2
Stimulated by the pioneering studies on the cationic porphyrin TMPyP4 and the natural product telomestatin, follow-up studies have developed, rapidly leading to a large diversity of macrocyclic structures with remarkable-quadruplex binding properties and biological activities. (hindawi.com)
Rapamycin, an immunosuppressive macrocyclic lactone, specifically inhibits the activity of mTOR. (biomedcentral.com)
Amino1
For example, a word in an open reading frame (ORF) has a direct influence on the primary amino acid sequence of a protein and hence is under strong selective pressure. (biomedcentral.com)
Stringent1
After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
Sequence1
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
Roles2
CCCTC-binding factor (CTCF) is a conserved transcription factor that performs diverse roles in transcriptional regulation and chromatin architecture. (preprints.org)
We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
Biological1
In this review we summarize the current state of the art in detailing the three main categories of quadruplex-binding macrocycles described so far (telomestatin-like polyheteroarenes, porphyrins and derivatives, polyammonium cyclophanes), and in addressing both synthetic issues and biological aspects. (hindawi.com)
Shown1
Further, it has been shown that polypyrimidine tract binding (PTB) protein acts as an important regulator of p53 IRES activities. (iisc.ac.in)
Dose1
Cialis Daily, these low dose tablets are specifically for daily use and provide a continuous level of treatment. (aetechnologies.com)
Expression1
We show that HBV replication induces the expression of angiogenic proteins interleukin 6 (IL6) and cyclooxygenase-2 (Cox2). (akjournals.com)
Transcription1
Treating SMMC-7721 cells with Rapamycin, a specific mTOR inhibitor, significantly reduce hTERT protein level but did not affect hTERT transcription. (biomedcentral.com)
Recognition1
Specifically targeting certain somatic cells circumvents immune recognition and bypasses the need to revert back to an embryonic state thus avoiding the loss of essential age-related and epigenetic factors. (vectorbuilder.jp)
Liver1
UCLA-led research describes the role that a protein called CEACAM1 plays in protecting the liver from injury during the transplantation process, potentially improving transplant outcomes. (technologynetworks.com)
Tissue1
Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
Level1
While the level of p53 protein is maintained low in unstressed conditions, there is a rapid increase in the functional p53 protein levels during stress conditions. (iisc.ac.in)
Results1
Specifically, HBx alone results in dimer formation between NFκB and RelA, while the combined presence of HBx and ibuprofen leads to the formation of NFκB and C-rel. (akjournals.com)
Important2
The Brassica family contains several economically important crops, including rapeseed (Brassica napus, 2n = 38, AACC), the second largest source of seed oil and protein meal worldwide. (preprints.org)
In an important model of premature aging, Werner's Syndrome, the genetic problem seems to be lack of a helicase protein that can unwind G-quadruplexes. (anti-agingfirewalls.com)
Result1
Furthermore, because MGMT protein loss may be a result of promoter hypermethylation, it was reported that methylation of the MGMT promoter in gliomas is a useful predictor of the responsiveness to alkylating agents ( 7 ). (aacrjournals.org)
Cells1
It is now well documented in literature that p53 protein accumulates in the cells following DNA damage by posttranslational modifications leading to increased stability and half life of protein. (iisc.ac.in)
Activation1
Dipeptidyl peptidase (DPP) 9, DPP8, DPP4 and fibroblast activation protein (FAP) are the four enzymatically active members of the S9b protease family. (preprints.org)
Assembly1
The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)