SerinePhosphorylationAmino Acid SequenceMolecular Sequence DataMutagenesis, Site-DirectedBinding SitesCell LineMutationPhosphoserineSerine EndopeptidasesProtein KinasesProtein-Serine-Threonine KinasesProtein BindingKineticsThreonineProtein Structure, TertiaryTyrosineSequence Homology, Amino AcidBase SequencePeptide MappingRecombinant ProteinsPhosphoproteinsModels, MolecularSubstrate SpecificityEnzyme ActivationPhosphopeptidesAmino Acid SubstitutionProtein ConformationPeptide FragmentsProtein Kinase CCasein Kinase IIStructure-Activity RelationshipProtein Processing, Post-TranslationalTransfectionSerine ProteasesSerine Proteinase InhibitorsAmino AcidsElectrophoresis, Polyacrylamide GelCloning, MolecularCyclic AMP-Dependent Protein KinasesCysteineRecombinant Fusion ProteinsSequence AlignmentCOS CellsCells, CulturedEscherichia coliPhosphoamino AcidsSignal TransductionAlanineProtein-Tyrosine KinasesPeptidesTrypsinMolecular WeightAmino Acid MotifsProtein Structure, SecondaryPhosphothreonineBlotting, WesternEnzyme InhibitorsDNA-Binding ProteinsHeLa CellsCatalysisProteinsPhosphoprotein PhosphatasesCasein KinasesPhosphotyrosineCattlePrecipitin TestsCatalytic DomainConserved SequenceOxidative PhosphorylationCell MembraneMembrane Proteins14-3-3 ProteinsPoint MutationCarrier ProteinsDNA PrimersCalcium-Calmodulin-Dependent Protein KinasesChromatography, High Pressure LiquidMass SpectrometryCricetinaeImmunoblottingProto-Oncogene ProteinsMutagenesisDNABacterial ProteinsHistidineProto-Oncogene Proteins c-aktOkadaic AcidCrystallography, X-RayMitogen-Activated Protein KinasesImmunoprecipitationCytoplasmAdenosine TriphosphateIntracellular Signaling Peptides and ProteinsPlasmidsIsoenzymesCercopithecus aethiopsDNA, ComplementaryProtein TransportTetradecanoylphorbol Acetate