Specific nucleic acid probe. Medical search. Frequent questions

Human satellite I sequences include a male specific 2.47kb tandemly repeated unit containing one Alu family member per repeat. (wikipedia.org)
Localisation of satellite DNA sequences on human metaphase chromosomes using bromodeoxyuridine-labelled probes. (wikipedia.org)
Primers for RT-LAMP or RT-RPA can be designed for specific viral RNA sequences, and the amplification methods are used before the Cas enzyme detection step, to multiply detection signals from the specific nucleic acid sequences. (nature.com)
The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)
Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
The highly interdisciplinary project aims to develop cell membrane permeable AdoMet analogs and use them for DNA or RNA modifying enzymes which can position fluorescent probes at the specific sequences. (uni-goettingen.de)
A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
Molecular beacon probes can be custom designed to target specific DNA or RNA sequences, which allows molecular beacons to be used for real-time detection and quantification of DNA and RNA. (edinst.com)
Molecular diagnostics are used to asses an individual's health condition at the molecular level, analyzing specific genetic sequences in DNA, ribonucleic acid (RNA), or the material or protein that is encoded in these genetic sequences, says Jiang. (medicaldesignbriefs.com)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
The principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid molecules. (tutorialspoint.com)
The probes are complementary to specific sequences of DNA or RNA molecules that are to be analysed. (tutorialspoint.com)
The hybridization occurs between the complementary sequences of the probes and the target molecules. (tutorialspoint.com)
The cDNA microarray uses complementary DNA as probes, while the oligonucleotide microarray uses short DNA sequences as probes. (tutorialspoint.com)
An RLU measurement less than the Cutoff Value indicates the absence of the specific HPV DNA sequences tested or HPV DNA levels below the detection limit of the assay. (cdc.gov)
Incorporating Locked Nucleic Acid into oligonucleotides increases thermal duplex stability 2 and improves the specificity of oligonucleotide hybridization to target sequences. (sigmaaldrich.com)
The bold letters in the sequences denote Locked Nucleic Acid bases. (sigmaaldrich.com)
Specifically, Locked Nucleic Acid oligonucleotides can be designed to address traditionally problematic target sequences, such as AT- or GC-rich regions. (sigmaaldrich.com)
The detection and quantitation of specific nucleic acid (NA) sequences continue to grow in importance. (dcu.ie)
This Thesis reports on novel, ultrasensitive methods to detect specific NA sequences without the need for amplification of the target, e.g., using PCR. (dcu.ie)
PCR primed with VNTR core sequences yields species specific patterns a" by Daniel D. Heath, George K. Lwama et al. (uwindsor.ca)
This was achieved by developing a multiplex PCR assay with primers and probes specific to different regions of the HBB DNA, G6PD mRNA and 18S rRNA sequences. (auckland.ac.nz)
This process is repeated to build specific oligonucleotide sequences. (medscape.com)

The assay can also be used as an analyte-specific reagent on urine or urethral swabs from men, if validated in a CLIA lab. (genomeweb.com)
Here, we report the clinical validation of the specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) assay using the enzyme Cas13a from Leptotrichia wadei for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-the virus that causes coronavirus disease 2019 (COVID-19)-in 154 nasopharyngeal and throat swab samples collected at Siriraj Hospital, Thailand. (nature.com)
The GEN-PROBE APTIMA trichomonas vaginalis assay combines the technologies of target capture, Transcription-Mediated Amplification (TMA), and Dual Kinetic Assay (DKA). (cdc.gov)
The assay is based on a novel nucleic acid probe that is cleaved by an RNase enzyme specific to the salmonella species. (labmanager.com)
Based on this highly specific enzymatic recognition, the team first developed a fluorescence-based assay on salmonella RNase H, and then extended the principle to a simple, portable salmonella assay based on a colloidal gold colorimetry. (labmanager.com)
In a slight departure from this methodology, however, the team did not use a paper strip as the basis for their assay, but instead turned to plastic pipette tips, which are commonly used in the laboratory to measure specific amounts of liquids. (labmanager.com)
The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
Demonstration of Blastomyces -specific nucleic acid or proteins in a clinical specimen or isolate using a validated molecular assay (e.g. (cdc.gov)

Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
Development of commercially available molecular methods, such as nucleic acid hybridization probes, improved turnaround times significantly, but can lack specificity. (bruker.com)

Within a detection limit of 42 RNA copies per reaction, SHERLOCK was 100% specific and 100% sensitive with a fluorescence readout, and 100% specific and 97% sensitive with a lateral-flow readout. (nature.com)
For the full range of viral load in the clinical samples, the fluorescence readout was 100% specific and 96% sensitive. (nature.com)
Advances in fluorescence microscopy, fluorescent dyes, biotechnology, bioinformatics, and research on the human genome project in the late 90's accelerated the development of methods to synthesize and design NA probes for FISH. (springer.com)
The intensity of fluorescence is measured to quantify the amount of hybridization that has occurred between the probes and the target molecules. (tutorialspoint.com)
Here, we report for the first time a species-specific peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method for Mycobacterium immunogenum, a non-tuberculous Mycobacterium species prevalent in MWF and implicated in occupational lung disease hypersensitivity pneumonitis and pseudo-outbreaks. (cdc.gov)
Fluorescence Probing, Da An Gene Co. (bvsalud.org)

The microarray uses an antibody directed against a specific protein target to determine protein expression, though the tool can also be adapted to other protein-specific probes, such as labeled peptides, proteins, nucleic acids, or drugs. (genomeweb.com)
Gel electrophoresis is a common laboratory technique to isolate nucleic acids and proteins. (thermofisher.com)
Running a gel, formally called gel electrophoresis, is an effective method to separate biomolecules, such as nucleic acids and proteins , based on size. (thermofisher.com)

Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
Such tests use a nucleic acid hybridization technique that is based on double-stranded complementary pairing of nucleic acids. (iso.org)
Basically, technicians synthesize a single-stranded nucleic acid sequence complementary to the DNA or RNA of a specific pathogen as a probe. (iso.org)
This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)
It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
initially the capture NA strand is immobilised onto the electrode surface, this is then hybridised to the complementary target sequence, which is then followed by target hybridisation to a complementary probe strand which is labelled with a nanoparticle. (dcu.ie)
A piece of single-stranded nucleic acid that hybridizes specifically to the complementary sequence of RNA or DNA in the sample. (cdc.gov)

Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)
Table 1 Increasing the number of Locked Nucleic Acid bases in an oligonucleotide increases the T m . (sigmaaldrich.com)
The presence of a single base mismatch has a greater destabilizing effect on the duplex formation between an Locked Nucleic Acid oligonucleotide and its target than with a native-state DNA oligonucleotide. (sigmaaldrich.com)

BioGenex is pleased to launch two Super Sensitive TM Nucleic Acid miRNA in situ hybridization (SSNA miRNA ISH) probes and automated systems for differentiation of wild type and BRCA mutated breast cancer. (biogenex.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)

Methods that directly detect bacteria from clinical specimens include acid fast and fluorescent smears, culture, and nucleic acid amplification such as polymerase chain reaction (PCR). (vin.com)
Both amplification products are detected through fluorescent probes against the generated nucleic acids. (vin.com)
It involves chemical synthesis of the cofactor analogues and mutagenesis of the enzymes acting on nucleic acids combined with selection of the variants which can incorporate fluorescent labels into natural DNA or RNA. (uni-goettingen.de)
Bucevičius J, Kostiuk G, Gerasimaitė R, Gilat T, Lukinavičius G (2020) Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect. (uni-goettingen.de)
The DNA or RNA molecules that are labelled with fluorescent dyes are hybridized to the probes on the microarray chip. (tutorialspoint.com)
The cDNA fragments are then labelled with fluorescent dyes and hybridized to the probes on the microarray chip. (tutorialspoint.com)
The sample is labeled with fluorescent dyes, either directly or indirectly, and then hybridized to the probes on the microarray chip. (tutorialspoint.com)
Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)

The new guidelines further mention two specific antigen detection and nucleic acid probe-hybridization tests. (genomeweb.com)
Viral-antigen detection is one of the new tests, but the results are generally less sensitive and less specific than those of conventional cell cultures. (medscape.com)
Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)

We evaluated the effect of time of specimen collection on culture yield following a positive nucleic acid amplification test (NAAT). (bmj.com)
When using this method of diagnosis, specimen collection must occur prior to acetic acid or iodine use if colposcopy is to follow. (medscape.com)

[ 65 ] and serologic tests, can be used to follow the increase in specific serum antibodies and for diagnostic purposes. (medscape.com)
BioGenex miRNA ISH Panel Probes are currently one of the best products for complex diagnostic assays. (biogenex.com)
We then developed a specific diagnostic PCR for Protochlamydia spp. (cdc.gov)
These results suggest that real-time NASBA may represent a sensitive and specific diagnostic procedure for piscine nodaviruses. (int-res.com)
Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
The emergence of SARS-CoV-2 has underlined the need for rapid and easy-to-implement diagnostic nucleic acid detection methods. (medrxiv.org)

The long-term objective of this project is to develop effective means for the detection and identification of new and emerging plant viral and bacterial diseases of ornamentals, thus allowing growers to select pathogen-free or pathogen-indexed plants (tested for absence of specific pathogens) for propagation. (usda.gov)
Some real-world examples are PCR quantification, in vivo RNA detection, pathogen detection, viral load quantification and study nucleic acid-protein interactions. (edinst.com)
CONCLUSION: This review, therefore, explores in depth the applications of non-viral to viral proteinbased noncarriers with a specific focus on intracellular drug delivery and anti-cancer drug targeting ability. (bvsalud.org)

ABSTRACT: Nucleic acid sequence based amplification (NASBA) is an isothermal nucleic acid amplification procedure based on target-specific primers and probes, and the co-ordinated activity of 3 enzymes: AMV reverse transcriptase, RNase H, and T7 RNA polymerase. (int-res.com)

A mixture of reagents composed of nanogold-DNA and the DNA-RNA probe were then sucked up into the pipette tip, causing a double layer of nanogold to form on the walls, because the DNA-RNA hybrid probe links both layers. (labmanager.com)
These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane. (aidstar-one.com)

Nucleic acid detection by isothermal amplification and the collateral cleavage of reporter molecules by CRISPR-associated enzymes is a promising alternative to quantitative PCR. (nature.com)

Specific and rapid detection and quantification of mycobacteria in contaminated metalworking fluid (MWF) are problematic due to complexity of the matrix and heavy background co-occurring microflora. (cdc.gov)

BioGenex unique SSNA miRNA ISH probes enable detection of single nucleotide mismatch thus allowing high sensitivity and specificity with a clean intense staining. (biogenex.com)
But it also covers the steps of the overall process of pre-examination, examination and post-examination for the analysis of nucleic acid amplification method for the detection of SARS-CoV-2, and specifies the methods and comprehensive assessment indicators including accuracy, limit of detection, inclusivity, and specificity, etc. (iso.org)
The updated algorithm features a specific sequence of tests to provide maximal sensitivity, specificity, and accuracy for HIV detection. (hivguidelines.org)
Nucleic acid amplifications tests (NAAT) such as multiplex reverse-transcriptase polymerase chain reaction (RT-PCR) remain the test of choice 3 for the detection of respiratory viruses due to their sensitivity, specificity, and time to virus detection. (medrxiv.org)
A novel species-specific 14-bp PNA probe was designed for M. immunogenum based on its 16S rRNA gene sequence and was validated for specificity, by testing against a panel of other phylogenetically closely related rapidly growing mycobacteria and representative species of gram-positive, gram-negative, and acid fast organisms. (cdc.gov)

Although acid fast smears of clinical specimens or histologic examination of tissues can detect mycobacteria, species identification requires either confirmation by culture or DNA probes. (vin.com)
However, in clinical trials, these tests have shown approximately a 99% sensitivity at detecting acid fast positive, culture positive samples, but only a 60-80% sensitivity at detecting acid fast negative, culture positive samples. (vin.com)
Each SomaPlex microarray contains a serum sample with complete demographic and relevant clinical disease-specific data. (genomeweb.com)
Her clinical manifestations included specific facial features, moderate mental retardation and frequent seizures. (spandidos-publications.com)

Recent technological advances have seen the introduction of methods that detect mycobacteria by amplification of mycobacterial nucleic acids (DNA or RNA) followed by detection of the amplification product. (vin.com)
At present, two amplification methods have been FDA approved for detection of M. tuberculosis from sputum in humans: the Amplicor system (Roche Molecular Systems, Branchburg, NJ) and the AMTD system (Gen-Probe Inc., San Diego, CA). The Amplicor system has as its target the mycobacterial 16S ribosomal RNA gene. (vin.com)
There are currently three methods for detecting SARS-CoV-2: nucleic acid tests, antigen tests and antibody tests. (iso.org)
The nucleic acid test is the 'gold standard' for pathogenic diagnosis, faster and more accurate than the other methods. (iso.org)
ISO/TS 5798 mainly focuses on the use of nucleic acid amplification methods for diagnosis and screening of SARS-CoV-2 and proposes requirements and recommendations for the design, development, validation, verification and implementation of testing methods. (iso.org)
Several methods have been devised recently for very sensitive detection of variola virus DNA and to distinguish this DNA from that of other orthopoxviruses, the most promising being analysis by polymerase chain reaction (PCR) of restriction fragment length polymorphisms, multiplex PCR and real-time PCR with fluorigenic probes. (who.int)

The prefix "deoxy" distinguishes DNA from its close relative RNA (ribonucleic acid). (wikibooks.org)

Methyltransferase-assisted labeling of nucleic acids relies on cofactor S Adenosyl L methionine (AdoMet) analogs where transferrable methyl group has been replaced with extended functionalized chains. (uni-goettingen.de)
A variety of methodologies for labeling DNA are used to generate end-labeled or continuously labeled probes. (enzolifesciences.com)
Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
Nick translation (NT) is one of the oldest probe labeling techniques. (enzolifesciences.com)

The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
The polymerase chain reaction (PCR) and its variants are analytical gold standards for nucleic acid-based testing that play a critical role in diagnosing and monitoring infectious diseases. (selectbiosciences.com)
DNA is a nucleic acid which is capable of duplicating itself via the enzyme known as DNA polymerase. (wikibooks.org)

Molecular beacon probes are a sequence of nucleotides (the building blocks of DNA and RNA) that can be used to fluorescently detect the presence of a specific sequence of DNA or RNA. (edinst.com)
In developing a probe, a sequence of nucleotides must be identified, isolated, reproduced in sufficient quantity, and tagged with a label that can be detected. (enzolifesciences.com)

Importantly, the CDC also now encourages the use of "highly sensitive and specific tests" for both women and men, as opposed to the less sensitive microscopy method, called a wet prep, and culture. (genomeweb.com)
Develop a biological knowledge base common to contemporary biologists, encompassing microscopy, cell structure, cellular membranes, and nucleic acids biology. (uaeu.ac.ae)
AFB-smear microscopy is not specific for M. tuberculosis complex. (cdc.gov)

BioGenex miRNA probes are dual-end labeled on 3' and 5' with five FAM molecules that amplify the signal, giving intense stains. (biogenex.com)
During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
In this work, we use isotope incorporation within DNA molecules to model taxon-specific population growth in the presence of 18 O-labeled water. (osti.gov)
In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)

The probes can be cDNA fragments or oligonucleotides, depending on the type of microarray. (tutorialspoint.com)

Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. (aidstar-one.com)
Deoxyribonucleic acid solution from calf thymus is suitable for use as a blocking agent in Southern hybridizations. (thomassci.com)

Culture for Neisseria gonorrhoeae in asymptomatic, nucleic acid amplification test (NAAT)-positive extragenital infections usually has a low yield and is not frequently performed. (bmj.com)

Specimens containing the target DNA hybridize with the HR HPV RNA probe cocktail. (cdc.gov)
Qualitative detection of nucleic acids within the envelope (E) gene specific for SARS coronavirus 2 (SARS-CoV-2) in respiratory specimens by nucleic acid amplification with probe-based detection. (cdc.gov)

Three chromosome-specific repetitive probes labeled with either amino acetyl fluorene (AAF), mercury, or biotin were hybridized simultaneously to metaphase chromosomes prepared from human blood lymphocytes or to interphase tumor nuclei. (nih.gov)
This paper reports a validated Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) method to simultaneously quantify TRP, L-kynurenine (KYN), xanthurenic acid (XA), 3-hydroxykynurenine (3OHKYN), kynurenic acid (KA), 3-hydroxyanthranilic acid (3OHAA), anthranilic acid (AA), 5-hydroxytryptamine (serotonin, 5HT) and tryptamine (TRYP) in Dulbecco's Modified Eagle and Eagle's Minimum Essential Media (DMEM and EMEM, respectively). (bvsalud.org)
Multiplexed FISH is often used to locate multiple targets in a cell nucleus simultaneously using probes of different colors, in order to study sparse cytological substrates (Lichter 1997 ). (springer.com)
In addition, the cells were simultaneously probed for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin. (fsu.edu)

The individual DNA strands are called probes. (medscape.com)

Nucleic acid electrophoresis uses a gel matrix to separate DNA and RNA fragments based on size and molecular weight. (thermofisher.com)
Electrophoresis is a common lab technique used to identify, quantify, and purify nucleic acid fragments and assess quality. (thermofisher.com)
When the amplified fragments were used as probes with genomic DNA Southern blots they produced hypervariable singlelocus or few-locus patterns in fish, birds, and humans. (uwindsor.ca)

The Selection Reagent differentiates hybridized from unhybridized probe, eliminating the generation of signal from unhybridized probe. (cdc.gov)
Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. (aidstar-one.com)

They would then label it using, for example, radioisotopes or enzymes, and subject it to hybridization with the nucleic acids of the pathogen to be tested. (iso.org)

Because of small differences in the 16S ribosomal gene, the primers are specific to organisms only of the M. tuberculosis complex ( M. tuberculosis, M. bovis, M. africanum, M. microti ) and only these species are detected. (vin.com)
Research over the past decade has resulted in the development of two commercial interferongamma release assays (IGRAs), based on the principle that the T-cells of individuals who have acquired TB infection respond to re-stimulation with Mycobacterium tuberculosis-specific antigens by secreting interferon. (bvsalud.org)

While culture tests can take days, molecular probe assays can be performed within a few hours or minutes. (enzolifesciences.com)

The multiplex ligation‑dependent probe amplification project of the Angel/chubby and copy number variation (CNV) sequencing were assessed concomitantly to identify the pathogenic genes responsible for the development of AS. (spandidos-publications.com)

As the team report in the journal Angewandte Chemie, this specific enzymatic cleavage principle made it possible to build a sensitive but simple and portable test system using colloidal gold. (labmanager.com)

Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon. (aidstar-one.com)
DNA stands for deoxyribonucleic Acid . (wikibooks.org)

Nucleic acid testing, which operates on this molecular level, has gradually become the mainstream of pathogen detection. (iso.org)
If a probe can be complementarily paired with the nucleic acid of the pathogen to be tested, it is possible to observe the signals of the markers, so that the species of the pathogen to be tested can be confirmed. (iso.org)

ISH was first demonstrated by Gall and Pardue ( 1969 ) using radioactive rRNA probes to visualize extrachromosomal rDNA. (springer.com)

Structure of Locked Nucleic Acid and native-state DNA monomers. (sigmaaldrich.com)

The ability of oligonucleotides to discriminate between alleles via SNPs is greatly enhanced by the incorporation of Locked Nucleic Acid bases 6-8 ( Figure 2 ). (sigmaaldrich.com)
Shorter oligonucleotides incorporating Locked Nucleic Acid bases can be used at the same temperatures as longer native-state DNA oligonucleotides. (sigmaaldrich.com)
Pink) mutant DNA analysis with Locked Nucleic Acid mutant probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
Purple) wild-type DNA with Locked Nucleic Acid mutant Probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
With Locked Nucleic Acid oligonucleotides, the selective placement of Locked Nucleic Acid bases facilitates the optimal design of highly-specific, shorter oligonucleotides that perform well, even at lengths of only 13 to 20 bases. (sigmaaldrich.com)
DNA is a long chain of linear polymers containing deoxyribose sugars and their covalently bonded bases known as nucleic acids. (wikibooks.org)
In DNA a sequence of three bases, which is called a codon, is responsible for the encoding of a single amino acid. (wikibooks.org)

Microarray analysis is a method that uses microchips containing anchored arrays of short DNA elements (known as probes) for the large-scale interrogation of gene expression. (nature.com)
[ 6 ] Both technologies use hybridization of labeled nucleic acid transcripts to measure the gene expression. (medscape.com)

Frommer then determined the chromosomal locations of the major simple-sequence repeats by devising a new method of non-radioactive labelling, based on incorporation of bromodeoxyuridine into single-stranded probes. (wikipedia.org)

Acid fast smears have a lower limit of detection of about 1000 organisms/ml, whereas culture can detect approximately 100 organisms/ml. (vin.com)

Detection and typing of HPV DNA in vaginal swabs (in conjunction with testing of NHANES sera for HPV antibody) will allow evaluation of trends in prevalence of type-specific HPV infection by age, sexual behavior, and race/ethnicity. (cdc.gov)

Using a multi-round selection process, the McMaster team uncovered an artificial DNA-RNA hybrid probe that is a substrate for a salmonella-specific form of an RNase H enzyme. (labmanager.com)
The technology involves the preparation of a microarray chip, which consists of a solid substrate, usually glass or silicon, with thousands or millions of immobilized DNA probes. (tutorialspoint.com)
The microarray chip is prepared by depositing DNA probes onto a solid substrate, usually a glass slide or silicon wafer. (tutorialspoint.com)
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. (aidstar-one.com)
Immobilized hybrids are then reacted with alkaline phosphatase conjugated antibodies specific for the RNA: DNA hybrids, and detected with a chemiluminescent substrate. (cdc.gov)

Currently, a range of probes can be synthesized to locate and quantify specific short RNAs, genes, entire chromosomes, and even cells (Evanko 2007 ). (springer.com)

They are highly specific for TB complex organisms but cannot differentiate species. (vin.com)

Blood cells from these patients show defective induction of specific subsets of genes after exposure to microbial stimuli in vitro . (nature.com)
In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)

The optional MBT Mycobacteria IVD Module for the MALDI Biotyper ® is composed of a specific reference spectrum library and a software module. (bruker.com)
Samples flagged as mycobacteria will undergo an optimized acquisition method and spectra are automatically compared to the MBT Mycobacteria IVD Library, for sensitive and specific identification. (bruker.com)

Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)

The problem is exacerbated if non-sequence-specific probes, such as pH-sensitive dyes, are used for the detection 6 . (nature.com)
Bucevičius J, Gerasimaitė R, Kiszka KA, Pradhan S, Kostiuk G, Koenen T, Lukinavičius G (2022) A general, highly efficient and facile synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy. (uni-goettingen.de)

Nucleic acid amplification tests (NAATs) are the recommended test method. (cdc.gov)
It notably includes recommendations for use of nucleic acid amplification tests, or NAATs, for Trichomonas vaginalis and routine trichomonas screening for high-risk populations. (genomeweb.com)
Recently published, ISO/TS 5798 provides recommendations for the design, development, verification, validation and implementation of analytical tests for detecting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) using nucleic acid amplification. (iso.org)
This method is compatible with the standard workflow of conventional FISH, allows re-budgeting of the sample for various tests, and results in a ~ 15-fold reduction in probe consumption. (springer.com)

The amino acid is added to a growing protein during the process of translation. (wikibooks.org)

A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)

BioGenex SSNA miRNA probes combined with the automated processing using Xmatrx® greatly increases the reliability of the test results through the elimination of error-prone ISH procedure. (biogenex.com)
Most of us know that there are various types of COVID test available, but the new technical specification uses nucleic acid testing. (iso.org)
Nucleic Acid Amplification (NAA) test is an example of direct detection. (cdc.gov)

Diseases1

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes3

Information Science2

Health Care1

Sequences23

Assay10

Hybridization probes2

Fluorescence6

Proteins3

Complementary8

Oligonucleotide3

Situ hybridization2

Fluorescent8

Antigen detection4

Specimen2

Diagnostic7

Viral3

Primers and probes1

Reagents2

Isothermal amplification1

Quantification1

Specificity5

Clinical4

Methods6

Ribonucleic acid1

Labeling4

Polymerase4

Sequence of nucleotides2

Microscopy3

Molecules5

CDNA1

Hybridizations2

NAAT1

Specimens2

Simultaneously4

Strands1

Fragments3

Reagent2

Enzymes1

Tuberculosis2

Assays1

Multiplex1

Cleavage1

Deoxyribonucleic2

Pathogen2

RRNA1

Monomers1

Bases7

Gene expression2

Incorporation1

Detect1

Antibody1

Substrate5

Chromosomes1

Organisms1

Vitro2

Mycobacteria2

Substances1

Dyes2

Tests4

Amino1

Nucleotide sequence1

Test3