Specific mrnas cells localization of specific rnas. Medical search. Frequent questions

A. thaliana PHIP1 and its homologs represent a novel class of plant-specific RNA-binding proteins that may play a unique role in the polarized mRNA transport to the vicinity of the cell plate. (nih.gov)
Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. (elsevierpure.com)
In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. (biomedcentral.com)
RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors. (biomedcentral.com)
Here we demonstrate that RNA-binding proteins can be used to isolate mRNA populations representing total cell mRNA from specific types of cells, as well as discrete mRNA subpopulations that represent post-transcriptionally regulated subsets of mRNAs that encode functionally related proteins. (biomedcentral.com)
Our lab has devised biochemical and immunological approaches to gene expression profiling by using RNA-binding proteins as reporters of discrete mRNA subsets in metazoan cells [ 8 - 10 ]. (biomedcentral.com)
For example, we identified subpopulations of mRNAs that are associated with ELAV/Hu RNA-binding proteins that are expressed in specific cell types [ 10 ]. (biomedcentral.com)
While we and other labs have demonstrated the isolation of mRNA subsets that are potentially co-regulated using RNA binding proteins as reporters of gene expression, methods have not been described that provide information about coordinated posttranscriptional regulation within specific types of cells during tumorigenesis and development. (biomedcentral.com)
Moreover, because many different mRNA-binding proteins in specific cell types are known to interact with unique subpopulations of mRNAs encoding functionally related proteins [ 9 - 15 ] they can be informative of the dynamic effects of cells on one another. (biomedcentral.com)
We identified 410 proteins, including key DNA replication factors and proteins associated with transcription, chromatin organization, DNA repair and mRNA splicing. (umass.edu)
Around 25% of the proteins identified were of unknown function that might have the potential to be essential trypanosome-specific replication proteins. (umass.edu)
In primary murine pro B cells and MEFs isolated from Stat1-null mice the expression of all RNAs that encode mitochondria synthesized proteins are elevated 3 to 8 fold compared to pro B cells or MEFs derived from wild type littermates. (grantome.com)
The 3 spliced mRNAs putatively encode good proteins , altogether 3 different isoforms (1 complete, 2 partial ), some containing a coiled coil stretch [Psort2] . (nih.gov)
Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase. (stanford.edu)
Preliminary experiments suggest that by interacting with Argonaute2, PICK1 can relieve this block of protein synthesis and increase the production of specific proteins. (ukri.org)
MiRNAs silence target mRNAs via the RNA-induced silencing complex (RISC), of which Argonaute proteins (Ago) are the major subunit. (ukri.org)
RNA binding proteins (RBPs) regulate the diverse steps in RNA processing, including alternative splicing, which generates fiber-type specific isoforms of structural proteins that confer contractile sarcomeres with distinct biomechanical properties. (uni-muenchen.de)
The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including PRKRA, TRBP, and Dicer. (hindawi.com)
These proteins process pre-microRNAs into mature microRNAs (miRNAs) that target specific mRNA species for regulation. (hindawi.com)
Previously, we investigated the localization of several nuage-resident proteins in the nuage structures of spermatogenic cells by immunoelectron microscopy (IEM) [ 22 - 26 ]. (hindawi.com)
In addition, we determined that several nuage proteins localize to spermatogenic cell-specific structures, including mitochondria-associated granules (MAG), granulated bodies (GB), reticulated bodies (RB), and ribosome aggregates (RA) [ 22 - 26 ]. (hindawi.com)
Abbas YM, Pichlmair A, Górna MW, Superti-Furga G, Nagar B. Structural basis for viral 5'-PPP-RNA recognition by human IFIT proteins. (cemm.at)
De Keersmaecker K, Porcu M, Cox L, Girardi T, Vandepoel R, Op de Beeck J, Gielen O, Mentens N, Bennett KL, Hantschel O. NUP214-ABL1 mediated cell proliferation in T-cell acute lymphoblastic leukemia is dependent on the LCK kinase and various interacting proteins. (cemm.at)
CPA is sequence-specific and dependent on RNA-binding proteins (RBPs). (uni-frankfurt.de)
Mutation experiments involving the SRSF3- and SRSF7-specific binding motifs in the 3'UTR also confirmed the hypothesis of competition between the two SR proteins. (uni-frankfurt.de)
Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
These biomarkers are detectable in liquid biopsy, include circulating tumor cells (CTCs), proteins, cell free DNA (cfDNA) and RNA (cfRNA) that recently have acquired interest in cancer for their role in diagnosis and treatment response [ 1 , 3 ]. (scientificarchives.com)
KSRP is certainly a multifunctional, single-stranded nucleic acidity- Prinaberel (DNA- or RNA-) binding proteins. (iassist2012.org)
The proteins binds to AREs in the 3-UTR of mRNAs and recruits enzymes mixed up in 5- and 3-mRNA decay [10]. (iassist2012.org)
These antibodies react with a group of 35- to 40-kilodalton neuronal RNA-binding proteins, including HuD[4] , PLE21/HuC, and Hel-N1. (medscape.com)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)

U2AF1 is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. (wikipedia.org)
PHIP1 is a peripheral membrane protein and is localized at the cell plate during cytokinesis in plants. (nih.gov)
Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. (elsevierpure.com)
LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. (elsevierpure.com)
A comparison of their known functions has identified, besides a common role within protein folding, multiple roles for the cyclophilins within pre-mRNA splicing and cellular signalling, and within transcription and cell cycle regulation for the parvulins. (biomedcentral.com)
Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. (biomedcentral.com)
In this study, we define a model system for using poly (A) binding protein (PABP) to recover mRNAs from specific cell-types in mixed cell cultures. (biomedcentral.com)
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)
Previous work showed that the cell cycle regulator Cyclin B (CycB) is subject to translational repression in immature spermatocytes, mediated by the RNA-binding protein Rbp4 and its partner Fest. (stanford.edu)
Here we show that the spermatocyte-specific protein Lut is required for translational repression of cycB in an 8-hour window just before spermatocytes are fully mature. (stanford.edu)
In addition, spermatocyte-specific isoforms of Syncrip (Syp) are required for expression of CycB protein in mature spermatocytes and normal entry into the meiotic divisions. (stanford.edu)
Thus a set of spermatocyte-specific regulators choreograph the timing of expression of CycB protein during male meiotic prophase. (stanford.edu)
APA can potentially affect mRNA translation efficiency, localization, stability, and mRNA seeded protein-protein interactions. (stanford.edu)
Developmentally regulated alternate 3' end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage. (stanford.edu)
Transcriptomic analysis of ZAP-isoform-specific knockout cells revealed uncharacterized host mRNAs targeted by ZAPL/S with broad cellular functions such as unfolded protein response (UPR), epithelial-mesenchymal transition (EMT), and innate immunity. (bvsalud.org)
Polyglutamine-binding protein 1 ( PQBP1 ) is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by severe cognitive impairment, but also by physical defects that suggest PQBP1 has broader functions in embryonic development. (xenbase.org)
An intermediate between DNA and protein is called messenger RNA (mRNA), and neurons can transport mRNA to the parts of the neuron close to synapses and locally control the synthesis of a particular protein that is important for those synapses at a particular time. (ukri.org)
Another type of molecule, called micro RNA (miRNA) can bind to mRNA and stop the translation of mRNA into protein. (ukri.org)
We have found that a protein (called PICK1), which is known to be involved in synaptic plasticity over the timescale of hours, interacts with another protein (called Argonaute2), which is an important component of the cell machinery that promotes the association of miRNA with mRNA to block protein synthesis. (ukri.org)
Using these cells we will be able to understand more about the mechanisms that regulate the local control of protein synthesis in neurons in response to synaptic activity, and hence further our knowledge of the mechanisms that underlie long-term memory. (ukri.org)
The regulation of expression of X-box-binding protein-1 (XBP1), a transcriptional factor, involves an unconventional mRNA splicing that removes the 26 nucleotides intron. (mdpi.com)
PRKRA, a protein kinase, interferon-inducible double stranded RNA dependent activator protein kinase, also known as PACT, a protein activator of the interferon-induced protein kinase, is a product of PRKRA gene [ 1 , 2 ] and is a member of the dsRNA binding protein family [ 3 ]. (hindawi.com)
PRKRA is contained in the cytoplasmic RNA-induced silencing complex (RISC) together with transactivation response RNA binding protein (TRBP) and Dicer [ 4 - 6 ]. (hindawi.com)
The central dogma of biology is based on the concatenated transfer of information from DNA, via transcribed mRNA, to the translated protein. (uni-frankfurt.de)
We propose that the ability of mRNAs to self-sort into homotypic assemblies is an inherent property of an messenger ribonucleoprotein (mRNP) that is augmented under conditions that increase RNA concentration, such as upon enrichment in RNA-protein granules, a process that appears conserved in diverse cellular contexts and organisms. (princeton.edu)
Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)
Ribonucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a complex with the mRNA-destabilizing protein tristetraprolin (TTP) and a conserved adenylate-uridylate-rich element in the TF mRNA 3′ untranslated region. (imperial.ac.uk)
Whereas transcription elements and epigenetic systems are in Prinaberel charge of transcriptional control, posttranscriptional legislation (splicing, mRNA editing, balance, translatability, and localization) frequently depends upon RNA-binding protein (RBP) [4]. (iassist2012.org)
These protein either stabilize (HuR) or destabilize (AUF1, tristetraprolin, and KSRP) cytokine mRNAs by binding to particular sequence elements, frequently AU-rich components (ARE) situated in the 3 untranslated area (3-UTR) from the mRNA. (iassist2012.org)

In situ hybridization experiments showed that transcription of the P1 protamine mRNA starts in the middle of step 7 of spermiogenesis during substage VIIc. (rupress.org)
Although the in situ hybridization suggests a disappearance of protamine 1 mRNA after step 16 of spermiogenesis, Northern blot analysis shows that low levels of mRNA are present during the period of final condensation of the chromatin, reflecting the association of protamine with DNA. (rupress.org)
However, here using reverse transcription-PCR and in situ hybridization we show that mRNA encoding the ρ1 subunit is highly expressed in brainstem neurons. (jneurosci.org)
The probes are great for in situ hybridization and cell or tissue imaging applications requiring a short probe, such as in the detection of short sequence mutations or for discriminating between closely related sequences such as splice variants and isoforms. (qiagen.com)
RT-PCR and in situ hybridization (ISH) demonstrated that granulosa cells express wnt-2 mRNA. (elsevierpure.com)

It was further determined that up-regulation of ABHD11-AS1 expression plays an important role in chronic Cr(VI) exposure-induced cell malignant transformation and tumorigenesis, and the stemness of human lung cancer cells. (cdc.gov)
The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. (elsevierpure.com)
In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
Our results represent the first genome-wide survey of mRNAs localized to a specific cytoskeletal component and suggest that microtubule localization of specific mRNAs is likely to function in mitotic regulation and mRNA segregation during cell division. (escholarship.org)
During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2 period termed meiotic prophase. (stanford.edu)
The proper regulation of RNA processing is critical for muscle development and the fine-tuning of contractile ability among muscle fiber-types. (uni-muenchen.de)
m 6 A-dependent regulation of messenger RNA stability. (nature.com)
Integration of SRSF3- and SRSF7-specific iCLIP data suggested a possible competition between SRSF3 and SRSF7 at the proximal PAS (pPAS), which could thus act as a hotspot of 3'UTR regulation. (uni-frankfurt.de)
Previous studies have confirmed that miRNAs play an important role in gene regulation in CD44 + CD24 -/low breast cancer stem cells (BCSCs). (researchsquare.com)
DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
TF mRNA regulation by PARP-14 was selective, as tumor necrosis factor (TNF)α mRNA, which is also regulated by TTP, was not altered in PARP-14 deficient macrophages. (imperial.ac.uk)
These cytokines possess pleiotropic functions, like the regulation of immune cell activation and differentiation [1]. (iassist2012.org)

The subcellular localisation of translational repression via miRNA and RISC in neurons is unknown, and the mechanisms for activity-dependent modulation are also unclear. (ukri.org)
The miRNA profile between breast cancer stem cells(BCSCs, CD44+CD24-/low) and control MCF-7 breast cancer cells was obtained in a previous study. (researchsquare.com)
In our previous research, we performed miRNA profiling between sorted CD44 + CD24 -/low BCSCs and the control MCF-7 breast cancer cells [8]. (researchsquare.com)
In endometrial cancer only some of these cf-RNAs have been investigated, no studies have been performed on snoRNAs, piRNAs, snRNAs and tRNA, the most analyzed one is represented by cf-miRNA in plasma. (scientificarchives.com)
These findings open an immediate use of selected cf-miRNA as biomarker in liquid biopsy of EC and suggest further investigation of the other cf-RNAs to implement the knowledge in this fascinating field. (scientificarchives.com)
Even though the role of circulating non coding RNAs as miRNA and lncRNA can be easily understood taking into consideration that can be secreted and persist in biofluid in remarkably stable forms, as "free" or associated to exosomes, less is known concerning the role of circulating mRNA, mainly taking into consideration its instability and the presence of RNase, nevertheless its presence is detected in blood. (scientificarchives.com)
The quality and quantity changes of circulating RNAs, mainly miRNA, are broadly investigated and associated with the initiation and progression of cancer [ 5 ]. (scientificarchives.com)
Additionally, KSRP is certainly involved with different posttranscriptional procedures, such as legislation of mRNA splicing, balance, and translatability and microRNA (miRNA) maturation. (iassist2012.org)

For example, neurons tend to express mRNAs with long 3'UTRs due to preferential processing at PASs more distal than other PASs used in other cell types. (stanford.edu)
Nerve cells (neurons) in the brain communicate with one another at connections called synapses, which are located in small protrusions on the neuronal surface called dendritic spines. (ukri.org)
Interactome of two diverse RNA granules links mRNA localization to translational repression in neurons. (cemm.at)
RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)
The molecular link between splicing and mRNA localization/local translation has also been demonstrated in mammalian neurons. (frontiersin.org)
Specialized hypothalamic neurons secrete pulses of gonadotropin-releasing hormone (GnRH) into the portal blood vessels, which acts on the gonadotrophs to cause a pulsatile release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which act on ovarian follicle cells to control folliculogenesis. (glowm.com)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
C-E) Co-labeling VMHvl neurons for PR (anti--gal) and Cckar (mRNA) in PRPL/PL mice. (cdc.gov)
F) The vast majority of -gal+ (PR+) cells in various regions in PRPL mice are neurons, as evidenced by co-labeling with NeuroTrace (fluorescent Nissl) in the arcuate and NeuN for the remaining regions. (cdc.gov)

The goal of this study is to investigate the mechanism of Cr(VI)-induced lung carcinogenesis focusing on the role of the lncRNA ABHD11 antisense RNA 1 (tail to tail) (ABHD11-AS1). (cdc.gov)
It was found that the lncRNA ABHD11-AS1 expression levels are up-regulated in chronic Cr(VI) exposure-transformed human bronchial epithelial cells, chronically Cr(VI)-exposed mouse lung tissues, and human lung cancer cells as well. (cdc.gov)
Together, these findings indicate that lncRNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote cell malignant transformation and lung carcinogenesis. (cdc.gov)

In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)
This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
APA naturally occurs during development and cellular differentiation, with around 70% of human genes displaying APA in particular tissues and cell types. (stanford.edu)
Life depends on constant replenishment of human body cells with new cells created by differentiation of adult stem cells. (anti-agingfirewalls.com)
As Type D cells die from trauma or apoptosis they are replaced by new cells resulting from differentiation of Type B and Type C cells. (anti-agingfirewalls.com)
Because of their unique expression profile and special functions, miRNAs have been used as molecular markers to define the direction and processes of cell differentiation by regulating one or several target genes. (researchsquare.com)
In adaptive immune system replies, the cytokine environment Prinaberel is certainly very important to the activation and differentiation of Compact disc4+ T cells into specific effector T helper (Th) cell subsets (Th1, Th2, Th9, or Th17). (iassist2012.org)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Dynamic m 6 A mRNA methylation directs translational control of heat shock response. (nature.com)

The interaction of ABHD11-AS1 with SART3 promotes USP15 (ubiquitin specific peptidase 15) nuclear localization. (cdc.gov)
The family members consist of multiple functional domains, including a lysine-rich domain (KRD domain) that contains three nuclear localization motifs (KKKR/NK), two RNA recognition motifs (RRMs), and three CCHC-type zinc fingers. (nih.gov)
Both revealed nuclear localization. (umass.edu)

We demonstrate that in vivo mRNA localization to granules and self-assembly within granules are governed by different mRNA features: localization is encoded by specific RNA regions, whereas self-assembly involves the entire mRNA, does not involve sequence-specific, ordered intermolecular RNA:RNA interactions, and is thus RNA sequence independent. (princeton.edu)

Specifically, alternative cleavage and polyadenylation (APA) is a co-transcriptional molecular process that switches the polyadenylation site (PAS) at which a nascent mRNA is cleaved, resulting in mRNA isoforms with different 3'UTR length and content. (stanford.edu)
In addition, changes in APA mark a variety of pathological states, including many types of cancer, in which mRNAs are preferentially cleaved at more proximal PASs, causing expression of mRNA isoforms with short 3'UTRs. (stanford.edu)
Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of the 3' cleavage site, resulting in the production of mRNA isoforms with different length 3' UTRs. (stanford.edu)
We identified >500 Drosophila genes that express mRNA isoforms with a long 3' UTR in proliferating spermatogonia but a short 3' UTR in differentiating spermatocytes due to APA. (stanford.edu)
We showed that the ZAP isoforms regulated different UPR branches under resting and stress conditions and affected cell viability during ER stress. (bvsalud.org)
We also provided evidence for a different function of the ZAP isoforms in EMT-related cell migration, with effects that are cell-type dependent. (bvsalud.org)
Little quantitative information exists as to either how many transcript copies of particular genes are m 6 A modified ('m 6 A levels') or the relationship of m 6 A modification(s) to alternative RNA isoforms. (nature.com)
Initial determination of the poly(A)-tome in P19 cells by MACE-Seq yielded approximately 16,000 PAS and showed that slightly less than 50% of all genes used two or more PAS and expressed alternative 3'UTR isoforms. (uni-frankfurt.de)

This gene encodes a small (~35 kDa) subunit which plays a critical role in RNA splicing by recognizing and binding to AG nucleotides at the 3' splice site to facilitate spliceosome assembly. (wikipedia.org)
Extensive Co-IP experiments clearly demonstrated that only SRSF7, but not SRSF3, can interact with CFIm and FIP1 (a subunit from the CPSF complex) in an RNA-independent manner. (uni-frankfurt.de)

Long noncoding RNAs (lncRNAs), due to their multifunction in various biological processes, have been indicated to play a crucial role in CSC renewal and stemness maintenance. (nature.com)
While long non-coding RNAs (lncRNAs) are found abnormally expressed in cancer, how dysregulated lncRNAs contribute to carcinogenesis remains largely unknown. (cdc.gov)
Circulating RNAs are represented by a broad range of subtypes, such as microRNA (miRNAs), long non coding RNA (lncRNAs), messenger RNAs (mRNAs), transfer RNAs (tRNAs), snRNAs (small nuclear RNAs), snoRNA (small nucleolar RNAs), piwiRNAs (piRNA) and circRNAs (circular RNAs). (scientificarchives.com)
Circulating RNAs are represented by, such as for intercellular RNAs, a broad range of subtypes, mainly represented by microRNA (miRNAs), long non coding RNA (lncRNAs) and messanger RNAs (mRNAs). (scientificarchives.com)

We further show that splicing mediated by SF1 and Hrb87F is necessary for Z-disc stability and proper myofibril development, and strong knockdown of either gene results in impaired localization of kettin to the Z-disc. (uni-muenchen.de)
The length of the 3'UTR affects the stability, export efficiency, subcellular localization, translation rate, and local translation of the nascent transcript. (uni-frankfurt.de)
This was related to an increase in TF messenger RNA (mRNA) stability. (imperial.ac.uk)

Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
Noncoding RNA sequences can regulate gene expression via interactions with epigenetic and other control mechanisms. (stanford.edu)
RNA localization is of critical importance in many fundamental cell biological and developmental processes by regulating the spatial control of gene expression. (escholarship.org)
Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. (biomedcentral.com)
In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes. (biomedcentral.com)
However, the gene expression profile of a whole tumor corresponds to the combined profiles of the different cell types contained within it (e.g. endothelial cells, T-cells, cancer cells, stromal cells, etc. (biomedcentral.com)
Monitoring the dynamics of gene expression in each cell type of a tumor during angiogenesis will advance understanding of tumorigenesis as well as organogenesis, in general. (biomedcentral.com)
Therefore, it will be necessary to assess changes in gene expression that occur when cells such as tumor cells and endothelial cells interact in order to understand growth control and critical processes such as angiogenesis. (biomedcentral.com)
Using this approach, we were able to determine how the gene expression profiles of endothelial cells change in response to the presence of breast cancer cells. (biomedcentral.com)
Alternative processing of nascent mRNAs is widespread in eukaryotic organisms and greatly impacts the output of gene expression. (stanford.edu)
Stem cell gene expression evolves with age. (anti-agingfirewalls.com)
Although in principle stem cells can replicate indefinitely, in fact they age as the organism ages, continuing to change their gene expression. (anti-agingfirewalls.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

MicroRNAs (miRNAs) are small noncoding endogenous RNA molecules that repress target mRNAs through complementary binding in the message 3'-UTR. (ukri.org)
MiRNAs can also control the self-renewal or proliferation of stem cells [9]. (researchsquare.com)

We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors. (elsevierpure.com)
RISC assembles into processing bodies (P-bodies) which are specific cytoplasmic foci in somatic eukaryotic cells [ 7 - 9 ]. (hindawi.com)
On the other hand, there are specific cytoplasmic foci similar to the P-bodies in spermatogenic cells, which are called nuage [ 15 - 18 ]. (hindawi.com)

(12 ). However, the versatility of the system allows the use of any microRNA of choice, including neuron-specific microRNAs ( 14 ). (lu.se)

Cytotoxic T cell-mediated neuronal damage is suspected, although no animal models have been developed to confirm this. (medscape.com)

J Cell Biol (2016) 213 (5): 509-511. (rupress.org)
2016. J. Cell Biol . (rupress.org)
Cell Biol. (nature.com)
J Cell Biol (1988) 107 (2): 407-412. (rupress.org)
Nat Rev Mol Cell Biol , Art. (mpi-cbg.de)

Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

However, NBs are not passive byproducts of specific gene activity but act principally as sites of defined biochemical reactions and of gene activation or repression. (rupress.org)

We propose nucleosomes are assembled close to the replication fork followed by RNA pol II recruitment, transcription, and co-transcriptional RNA splicing. (umass.edu)
Transcription produces 3 alternatively spliced mRNAs . (nih.gov)
We use RT-PCR reverse transcription system and the expand high fidelity PCR system to detect spliced XBP1 mRNA, and fraction cells to determine the location of the unconventional splicing of XBP1 mRNA. (mdpi.com)
Transcription of a new pre-mRNA is terminated by hydrolytic cleavage in the 3'-UTR, and the newly formed 3'-end is protected from premature degradation by synthesis of a poly(A) tail. (uni-frankfurt.de)

Long noncoding RNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote lung carcinogenesis. (cdc.gov)

The effect of miR-20b-5p on xenograft growth was investigated in vivo by transfection of a lentivirus-overexpression vector into T47D cells. (researchsquare.com)
LO1-750 specifically identified necrotic core in ex vivo human coronary lesions.Injection of LO1-750 into high fat (HF) fed atherosclerotic Ldlr-/-mice led to specific focallocalization within the aortic arch and its branches, as detected by fluorescence moleculartomography (FMT) combined with micro-computed tomography (CT). (imperial.ac.uk)
Ex vivo confocalmicroscopy confirmed LO1-750 subendothelial localization of LO1-750 at sites ofatherosclerosis, in the vicinity of macrophages. (imperial.ac.uk)
In the rabbit, in vivo injected LO1-750 localization wassuccessfully imaged ex vivo in aortic lesions with a customised intra-arterial NIRF detectioncatheter. (imperial.ac.uk)

Human ZAP inhibits many viruses, including HIV and coronaviruses, by binding to viral RNAs to promote their degradation and/or translation suppression. (bvsalud.org)

Bioinformatics analysis revealed that ABHD11-AS1 levels are up-regulated in lung adenocarcinomas (LUADs) tissues and associated with worse overall survival of LUAD patients but not in lung squamous cell carcinomas. (cdc.gov)
Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)
We conducted RNA sequencing (RNA-Seq) of 103 tissues from rapeseed cultivar ZhongShuang11 (ZS11) at seven developmental stages (seed germination, seedling, bolting, initial flowering, full-bloom, podding, and maturation). (preprints.org)
Although widespread, the role of APA in the biology of cells, tissues, and organisms has been controversial. (stanford.edu)
Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
During the course of folliculogenesis, growth is achieved by cell proliferation and formation of follicular fluid, whereas development involves cytodifferentiation of all the cells and tissues in the follicle. (glowm.com)

The mRNAs appear to differ by truncation of the 5' end, presence or absence of a cassette exon , overlapping exons with different boundaries. (nih.gov)
In addition to the cis -elements in its 3′UTR and trans -acting factor Staufen, posterior localization of oskar mRNA requires deposition of exon junction complex (EJC) in its first exon through splicing ( Hachet and Ephrussi, 2004 ). (frontiersin.org)

The sequence of this gene is defined by 16 cDNA clones and 18 elements defined by RNA-seq, some from mixed (seen 7 times), embryo (5), l2 (once). (nih.gov)
Sequence-Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters. (princeton.edu)
Our Custom LNA mRNA Detection Probes are designed with optimal LNA positioning to achieve high sequence specificity, low secondary structure and minimal self-annealing. (qiagen.com)
Our user-friendly online design tool lets you easily design highly sensitive LNA probes for your specific mRNA sequence of interest. (qiagen.com)

Owing to a post-transcriptional shortening of the poly(A) tail by 130 bases, a decrease in the size of protamine 1 mRNA from approximately 580 to 450 nucleotides was observed in stages XIII-XIV suggesting an initiation of protamine 1 synthesis in step 13-14 spermatids. (rupress.org)

however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. (nature.com)
Previous studies have reported that the expression of several mitochondria encoded RNAs is inhibited as a result of incubation of cells with type one interferons (IFN alpha/beta). (grantome.com)
We hypothesize that Stat1 plays an important role in the expression of mitochondrial encoded mRNAs and possibly other functions involved in electron transport. (grantome.com)
To test this hypothesis we will perform the following specific aims: 1) Determine whether decreased expression of mitochondria-encoded RNAs is mediated by Stat1 localized in the mitochondria and identify the domains in Stat1 required for its localization in the mitochondria. (grantome.com)
2) Determine whether viral infection or IFN beta treatment of cells alters expression of mitochondria-encoded mRNAs and mitochondria respiration. (grantome.com)
E) Expression of pqbp1 and ncam mRNA in the neural plate of early neurula embryos. (xenbase.org)
We find that RBPs exhibit dynamic temporal and fiber-type specific expression patterns in mRNA-Seq data and display muscle-specific phenotypes. (uni-muenchen.de)
The expression of protamine 1 mRNAs has been localized in the very last phase of the haploid gene activity. (rupress.org)
Although macrophage TF expression is known to be regulated at the RNA level, very little is known about the mechanisms involved. (imperial.ac.uk)
The expression of wnt-2b transcripts in rat ovarian surface epithelium prompted us to examine whether the homologous gene is expressed in human ovarian cancer cell lines. (elsevierpure.com)
RT-PCR, using degenerate and specific primers for wnts, on RNA from five ovarian cancer cell lines confirmed the expression of transcripts for wnt-2b. (elsevierpure.com)
Furthermore, because wnts have been implicated in the oncogenic transformation of epithelial cells, our results raise the possibility that aberrant wnt expression may be involved in ovarian tumorigenesis in humans. (elsevierpure.com)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

Recent work has focused on the detection of neuron-specific ELAV mRNA in peripheral blood of SCLC patients using real-time quantitative polymerase chain reaction (PCR). (medscape.com)

N 6 -methyladenosine modification destabilizes developmental regulators in embryonic stem cells. (nature.com)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

It also binds specifically to Ran2 mRNA. (nih.gov)

The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been shown to hijack angiotensin converting enzyme 2 (ACE2) for entry into mammalian cells. (bvsalud.org)

N 6 -Methyladenosine (m 6 A) is a widespread, reversible chemical modification of RNA molecules, implicated in many aspects of RNA metabolism. (nature.com)

use an elegant transgene system in Drosophila melanogaster to manipulate the localization and activity of specific NB components without NB disassembly. (rupress.org)
One of the best-characterized examples is oskar mRNA which localizes to the posterior region of Drosophila oocytes ( Besse and Ephrussi, 2008 ). (frontiersin.org)

We established that endogenous ZAPL and ZAPS localize to membrane compartments and cytosol, respectively, and that the differential localization correlates with their target-RNA specificity. (bvsalud.org)
The above list is in order of increasing cell-type specificity and decreasing cell-type potency to differentiate into other cell types. (anti-agingfirewalls.com)
We found that cells exhibit a broad range of nonstoichiometric m 6 A levels with cell-type specificity. (nature.com)

High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis. (nature.com)

Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). (nature.com)
Nuclear localized USP15 interacts with pre-mRNA processing factor 19 (PRPF19) to increase CD44 RNA alternative splicing activating β-catenin and enhancing cancer stemness. (cdc.gov)
Then, agomir-20b-5p and antagomir-20b-5p were transfected into MCF-7 and T47D breast cancer cells to detect cell migration, wound healing and proliferation, and lentivirus vectors silencing or overexpressing miR-20b-5p were transfected into T47D-CSCs to detect proliferation and apoptosis. (researchsquare.com)
Oncogenic miR-20b-5p was confirmed to promote the malignant behaviors of breast cancer cells and BCSCs. (researchsquare.com)
Malentacchi F, Sorbi F, Cipriani N, Sgromo C, Antonuzzo L, Pillozzi S. Circulating Cell-Free RNA: A New Perspective for Endometrial Cancer. (scientificarchives.com)
Recently, circulating RNAs (cfRNAs) are increasing their importance as biomarkers in liquid biopsy for cancer patients both for tumor characterization and development and for setting a monitoring personalized therapy. (scientificarchives.com)
Two additional wnt transcripts (wnt-5a and wnt-11) were detected in the cancer cell lines and in the rat ovary. (elsevierpure.com)
We focus on the role of HPV in the increased incidence of oropharyngeal squamous cell carcinoma (OSCC), the head and neck cancer in which HPV is most commonly found ( 4 ). (cdc.gov)
Head and neck cancer most commonly is of the squamous cell carcinoma type (HNSCC) and includes cancers of the oral cavity, oropharynx, hypopharynx, larynx, sinonasal tract, and nasopharynx. (cdc.gov)
Although various malignancies have been reported in PEM, 80% of cases are associated with bronchial cancer, typically small cell lung carcinoma. (medscape.com)

This study was designed to examine whether the unconventional spicing of XBP1 mRNA could occur in the nucleus and its possible biological relevance. (mdpi.com)
Prior to active export factor-dependent transport from the nucleus to the cytosol, the newly formed pre-mRNA must mature. (uni-frankfurt.de)

The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)
The control of this transition from epithelial stem cell to differentiated corneocyte, which is abnormal in epidermal cancers, is not well understood. (stanford.edu)

APA-specific sequences include the poly(A) motif ('AAUAAA' and certain motif variants), the UGUA motif, and U/GU-rich sequences upstream and downstream of the poly(A) signal, respectively. (uni-frankfurt.de)

The results imply that, in addition to PAL, other lignin-specific enzymes and/or tissue structural changes may have more significant roles in tissue toughening of broccoli branchlets. (scialert.net)

possibility is that the damage is not induces mutations in a mutation integrated viral genome ( Todaro and repaired at al , and when the cell rep- assay permits it to be classified as Huebner, 1972) , and alteration of im- licates, the DNA polymerase correct- mutagenic. (who.int)

The differential affinity between SRSF3 and SRSF7 for polyadenylation factors could be attributed to two SRSF7-specific domains in subsequent mutation experiments: A CCHC-type Zn finger between the RRM and the RS domain, and a hydrophobic 27 amino acid long region in the middle of the RS domain. (uni-frankfurt.de)

Dr. Weatheritt opened his lab in 2018 focusing on combining data data approaches with CRISPR screening approaches and transcriptomics to understand the role of RNA in driving cellular phenotypes. (edu.au)

This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
We examined the consequences of CTCF depletion in immortalised human and mouse cells using shRNA knockdown and CRISPR/Cas9 genome editing and examined the growth and development of heterozygous Ctcf ( Ctcf +/- ) mice. (preprints.org)
For this purpose, various genome-wide methods, such as RNA-Seq, MACE-Seq, and iCLIP-Seq, were integrated and the findings were supported by reporter gene and mutation studies. (uni-frankfurt.de)

Moreover, using this approach we identify a set of endothelial genes that respond to the presence of co-cultured breast tumor cells. (biomedcentral.com)
AceView: gene:dpy-21, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView. (nih.gov)

N 6 -methyladenosine modulates messenger RNA translation efficiency. (nature.com)

In mouse embryos, cells seem prepatterned to become certain cell lineage because the first cleavage plane has been related with further embryonic-abembryonic axis at the blastocyst stage. (bioone.org)
To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
Surprisingly, we found that there is a significant percentage of the embryos (∼60%) with labeled and nonlabeled cells randomly distributed and intermingled. (bioone.org)
B-F) Tailbud tadpole stage embryos injected bilaterally at the two-cell stage with 50 ng control (CT) or pqbp1 (PQ) MOs as indicated (embryos in F received 100 ng MOs in total). (xenbase.org)
right panel, embryos injected dorsally with pqbp1 mRNA (2 ng) at the four-cell stage. (xenbase.org)

We found that the splicing factors hnRNPA1/A2, PTBP1/2, and U1-snRNP inhibit ZAPS production and demonstrated the feasibility to modulate the ZAPL/S balance by splice-switching antisense oligonucleotides in human cells. (bvsalud.org)
Alternative splicing of pre-mRNAs is an important means of regulating developmental processes, yet the molecular mechanisms governing alternative splicing in embryonic contexts are just beginning to emerge. (xenbase.org)
In murine P19 cells, we were able to demonstrate for the first time a direct link between 3'UTR-APA and nuclear export of mature mRNA by the splicing factors SRSF3 and SRSF7 and decipher the mechanism. (uni-frankfurt.de)

In stages II-VI (steps 16-18 spermatids), only the smaller size protamine 1 mRNA was detectable. (rupress.org)

To investigate how spindle-localized RNAs might influence mitosis, we comprehensively surveyed all messenger RNAs (mRNAs) that bound to microtubules during metaphase in both Xenopus laevis egg extracts and mitotic human cell extracts. (escholarship.org)
Pan, T. N 6 -methyl-Adenosine modification in messenger and long non-coding RNA. (nature.com)
The CDC's move came the day after the US Food and Drug Administration (FDA) approved Moderna's and Pfizer-BioNTech's updated COVID-19 messenger RNA (mRNA) vaccines for people 12 years or older and authorized the shots for emergency use in children 6 months through 11 years of age. (cdc.gov)

Methods have been devised to generate mRNA samples from specific types of tumor cells. (biomedcentral.com)

Using RT-PCR with degenerate primers on RNA from ovaries of hormone-stimulated immature rats, we identified transcripts for wnt-2 and wnt-2b. (elsevierpure.com)

The remaining cells in the follicle wall ( i.e. granulosa, membrana and periantral, theca, the theca interna and externa) develop into the corpus luteum. (glowm.com)
Further analyses using both RT-PCR and ISH indicated that granulosa cells express fzd-4 mRNA. (elsevierpure.com)
HN - 2008 BX - Von Ebner's Glands MH - Cumulus Cells UI - D054885 MN - A05.360.319.114.630.535.200.500 MN - A06.407.312.497.535.300.500 MN - A11.436.300.500 MS - The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. (bvsalud.org)
HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)

These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)

B cells are therefore particularly dependent on 'quality control' mechanisms to oversee antibody production. (cipsm.de)

cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

check also tag CHILD or specific HN - 2008 FX - Adolescent Nutrition FX - Infant Nutrition DH - Maternal Nutrition DI - 052505 MN - SP6.021.072 MS - Nutrition of a mother which affects the health of the INFANT as well as herself. (bvsalud.org)

Furthermore, since IL-4 mRNA decay was low in KSRP? (iassist2012.org)

http://dx.doi.org/10.1083/jcb.201504043 ) provide the first direct evidence that the histone locus body acts to concentrate key factors required for the proper processing of histone pre-mRNAs. (rupress.org)

Anatomy35

Organisms21

Chemicals and Drugs92

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Psychiatry and Psychology1

Phenomena and Processes75

Disciplines and Occupations2

Information Science4

Proteins34

Protein31

Situ Hybridization5

Regulation12

MiRNA8

Neurons10

LncRNA3

Differentiation12

Translational2

Nuclear localization3

Granules1

Isoforms8

Subunit2

LncRNAs4

Stability3

Gene expression13

MiRNAs2

Cytoplasmic3

MicroRNAs1

Neuronal1

Biol5

MicroRNA2

Repression1

Transcription4

Noncoding RNA1

Vivo4

Degradation1

Tissues7

Exon2

Sequence4

Nucleotides1

Expression13

Peripheral1

Embryonic stem2

Binds1

Mammalian cells1

Molecules1

Drosophila2

Specificity3

Methylation1

Cancer10

Nucleus2

Epithelial2

Sequences1

Enzymes1

Viral1

Subsequent1

Cellular1

Genome3

Genes2

Translation efficiency1

Embryos5

Splicing3

Detectable1

Messenger3

Tumor cells1

Immature1

Granulosa4

Isolate1

Dependent1

Interaction1

Affects1

Decay1

Histone1