RNAiGenesRescuesMutationsReverse geneticsDifferentialNeuronsSerotypesPharmacologicalCellularRibosomeNucleic acid sequencesMutantReceptorsLifespanPoliovirusIntestinalVitroConsistentChromatinTumorsHistoneTissuesInsulinManifestBiologicalGene of interestInteractionCellsAntibodiesTranscriptionExpressionMechanismsBasisDifferencesCellFindDisordersSimilarDataArticleHigherAbilityBrainGrowthDomain
RNAi4
- The discovery of gene silencing using double stranded RNA, also known as RNA interference (RNAi), and the development of gene knockdown using Morpholino oligos, have made disrupting gene expression an accessible technique for many more investigators. (wikipedia.org)
- citation needed] RNAi creates a specific knockout effect without actually mutating the DNA of interest. (wikipedia.org)
- citation needed] RNAi interference, specifically gene silencing, has become a useful tool to silence the expression of genes and identify and analyze their loss-of-function phenotype. (wikipedia.org)
- Chemical inhibitors were used on three targets in pharmacological screens using both planaria and schistosomula, revealing distinct motility phenotypes that were shown to correlate with planarian RNAi phenotypes. (biomedcentral.com)
Genes10
- citation needed] In the field of virology, reverse-genetics techniques can be used to recover full-length infectious viruses with desired mutations or insertions in the viral genomes or in specific virus genes. (wikipedia.org)
- On the basis of whole-transcriptome analyses, we identify many genes that are expressed in a sex- or life stage-specific manner and characterize the transcriptional landscape of a morphological region with unique biological adaptations, namely, bacillary band and stichosome, found only in whipworms and related parasites. (nature.com)
- We resolve chromosomal sequences and infer sex chromosome-specific parasite genes and new potential drug targets. (nature.com)
- Generation of molecularly defined null mutants revealed that loss of 8 out of 13 JmjC genes modify position effect variegation (PEV) phenotypes, consistent with their ascribed role in regulating chromatin organization. (sdbonline.org)
- In these cellular models, prolonged JHDM1B downregulation triggered a surge of 45S pre-rRNA transcription and processing, associated with a re-modulation of the H3K36me2 levels at rDNA loci and with changes in DNA methylation of specific CpG sites in rDNA genes. (oncotarget.com)
- However, the overall amount of H3K27me3 in C. elegans was reported to decrease with age, and RNA interference of the H3K27me3 demethylase UTX-1 prolonged lifespan by suppressing the expression of genes of the insulin pathway [ 16 ]. (aging-us.com)
- These extensive genetic screens led to identification of a number of genes that cause abormal cell lineage (the lin# genes) phenotypes, notably vulvaless (Vul)/Egg-laying defective (Egl) or multivulva (Muv) (Figure 3.1 ). (hideyuki-ts.com)
- For example, Zhang and Li found that housekeeping genes evolved more slowly than tissue-specific genes [ 10 ]. (biomedcentral.com)
- El objetivo de nuestro estudio tuvo como objetivo revisar y correlacionar genes involucrados en TEA y aque- llos relacionados con la comunicación ultrasónica en estudios con modelos animales de comportamiento social basado en el lenguaje en la base de datos PubMed. (bvsalud.org)
- As expected, PT isolated from the healthy kidney express a number of differentiation-specific genes that are associated with kidney function. (simonsfoundation.org)
Rescues2
- Further, constitutively active SRF partially rescues R62D-induced phenotypes. (jneurosci.org)
- While BUBR1 silencing rescues the mitotic phenotype induced by E2, p53 silencing or presence of E6/E7 (inactivating p53 and increasing BUBR1 levels respectively) both amplify it. (docksci.com)
Mutations2
- Mutations within the gene are induced and identified by various techniques and only subsequently is the mutant phenotype analyzed [ 1 ]. (biomedcentral.com)
- By contrast, mutations in noncoding regions do not provide significant growth advantages during tumor evolution ( 11 ). (spandidos-publications.com)
Reverse genetics4
- Reverse genetics is a method in molecular genetics that is used to help understand the function(s) of a gene by analysing the phenotypic effects caused by genetically engineering specific nucleic acid sequences within the gene. (wikipedia.org)
- While forward genetics seeks to find the genetic basis of a phenotype or trait, reverse genetics seeks to find what phenotypes are controlled by particular genetic sequences. (wikipedia.org)
- Reverse genetics attempts to connect a given genetic sequence with specific effects on the organism. (wikipedia.org)
- In contrast, directed reverse genetics is based on techniques that allow for specific inactivation of a gene. (biomedcentral.com)
Differential2
- Phase contrast (PC) and differential interference contrast (DIC) microscopy are the most popular label-free imaging techniques in biological research. (pressbrand.net)
- Differential interference contrast (DIC)/Nomarski microscopes were commonly used to examine the phenotypes because of their superior imaging quuality for live and unstained samples. (hideyuki-ts.com)
Neurons6
- Here, we showed that GM-CSF promotes bone cancer-associated pain by enhancing excitability of DRG neurons via the Jak2-Stat3-mediated upregulation of expression of nociceptor-specific voltage-gated sodium channels. (iasp-pain.org)
- In contrast, optogenetic manipulations of VTA DA neurons revealed their roles in sustaining recurrent scratching episodes through signaling scratching-induced reward. (iasp-pain.org)
- Overexpressing these mutant actins in mouse hippocampal neurons not only modulated growth-cone function but also neurite elongation, which was ambiguous by traditional pharmacological interference. (jneurosci.org)
- R62D-induced phenotypes strongly resemble neurons lacking SRF (Serum Response Factor). (jneurosci.org)
- The directed expression of Buffy in the dopamine producing neurons, via aDdc-Gal4 transgene, resulted in flies with increased climbing ability and enhanced survival, while the inhibition of Buffy in the dopaminergic neurons reduced climbing ability over time prematurely, similar to the phenotype observed in the alpha-synuclein-induced Drosophila model of PD. (sdbonline.org)
- The directed expression of Buffy in DA neurons provides protection and counteracts the alpha-synuclein-induced Parkinson disease-like phenotypes. (sdbonline.org)
Serotypes2
- Systematic vaccination of recruits against the 2 most common agents of ARD in the military, HAdV serotypes 4 and 7, decreased HAdV-specific respiratory illness by 95% to 99% and overall respiratory illness rates by 50% to 60% ( 11 - 13 ). (cdc.gov)
- Interference among the three attenuated poliovirus serotypes was minimized with a 'balanced- formulation' vaccine, and serologic responses after IPV were optimized by adjusting the antigenic content of each inactivated poliovirus serotype. (who.int)
Pharmacological2
- Moreover these actin mutants affect neurite elongation, an actin function which by pharmacological actin interference was ambiguous. (jneurosci.org)
- Taking advantage of experimental strategies including pharmacological tool and gene knockdown, we showed here that interference with JNK signaling pathway by targeting JNK1/2 or c-Jun reversed the mesenchymal properties of KB/VCR cells to epithelial phenotypes and suppressed the motile capacity of KB/VCR cells, such as migration and invasion. (biomedcentral.com)
Cellular6
- While RNA interference relies on cellular components for efficacy (e.g. the Dicer proteins, the RISC complex) a simple alternative for gene knockdown is Morpholino antisense oligos. (wikipedia.org)
- Taken together, these data indicate that the reduction of JHDM1B leads to a more aggressive cellular phenotype in mammary gland cells, by virtue of its negative regulatory activity on ribosome biogenesis. (oncotarget.com)
- These include the loss of proliferative control, enhanced cellular migration and invasion, extracellular matrix degradation, angiogenesis, lymphangiogenesis, and vascular invasion followed by the distant seeding of tumor cells in specific tissues, such as the liver or lung. (aacrjournals.org)
- For example, a phase 1 clinical trial using ultraviolet-attenuated Necator americanus larvae elicited an antigen-specific humoral and cellular immune response that resulted in reduced larval output in vaccinated participants [ 8 ]. (biomedcentral.com)
- EMT, a highly conserved cellular program in many important phases of embryonic development, is a biological process through which epithelial cells lose their epithelial cobblestone phenotype and acquire fibroblastic and mesenchymal-like phenotypes. (biomedcentral.com)
- In contrast to the influx of amino acids, the degradation of proteins is also the important intracellular mechanism for releasing free amino acids both under steady-state conditions and during cellular stresses. (spandidos-publications.com)
Ribosome2
- Epigenetic up-regulation of ribosome biogenesis and more aggressive phenotype triggered by the lack of the histone demethylase JHDM1B in mammary epithelial. (oncotarget.com)
- Currently the lab is focused on two specific systems: ERAD and ribosome UFMylation. (stanford.edu)
Nucleic acid sequences1
- Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
Mutant3
- The ability to analyze the loss-of-function phenotype allows analysis of gene function when there is no access to mutant alleles. (wikipedia.org)
- This success story is mainly founded on countless so-called forward genetic screens designed to elucidate gene functions on the basis of their mutant phenotypes. (biomedcentral.com)
- In a mutant of testis-specific TAF homolog (tTAF) that is responsible for nucleolus localization of Pc, dJmj signals were detected not only at nucleolus but also on the condensed chromatin in the late growth stage. (sdbonline.org)
Receptors1
- This effect on longevity is apparently correlated with the midgut senescence phenotypes as a result of direct hormone action through both hormone receptors expressed in the enteroblasts or other midgut cell types. (bioone.org)
Lifespan1
- The inhibition of porin under the control of neuronal Ddc-Gal4 result in short lifespan and in an age-dependent loss in locomotor function, phenotypes that are strongly associated with Drosophila models of Parkinson disease . (sdbonline.org)
Poliovirus1
- We recently reported a lack of interference between inactivated rotavirus vaccine (IRV) and inactivated poliovirus vaccine (IPV) and their potential dose sparing when the two vaccines were administered intramuscularly either in combination or standalone in rats and guinea pigs. (cdc.gov)
Intestinal1
- Intestinal macrophages display a "tolerant" phenotype, poorly responsive to toll like receptor (TLR) ligands (vital to avoid triggering inflammatory responses while scavenging commensal bacteria) whilst maintaining a homeostatic environment via IL-10 and PGE 2 production ( 7 ). (frontiersin.org)
Vitro1
- These functional changes were reversed by BMP7 and SMAD4 RNA interference in vitro . (aacrjournals.org)
Consistent1
- Alterations in functional variables (aggregation, wounding, motility, and invasion) following TGF-β1 treatment were consistent with a more invasive phenotype. (aacrjournals.org)
Chromatin1
- The PAH2 domain of the scaffold protein SIN3A interacts with the specific Sin3 Interaction Domain (SID) of several transcription factors, such as MAD1, bringing chromatin-modifying proteins such as histone deacetylases, and it targets chromatin for specific modifications. (bvsalud.org)
Tumors1
- What makes the response of PDAC to immunotherapy different from the responses of other solid tumors is the specific host tissue. (biomedcentral.com)
Histone1
- Immunocytochemical analyses was performed with specific antibodies to dJmj and tri-methylation at lysine 27 on histone H3 (H3K27me3). (sdbonline.org)
Tissues1
- Cre or Flp recombinases can be induced with chemical treatments, heat shock treatments or be restricted to a specific subset of tissues. (wikipedia.org)
Insulin1
- Furthermore, these senescence phenotypes appear to be independent of insulin signaling and manifest in an organ-specific manner. (bioone.org)
Manifest1
- Depending on the specific enzyme affected, porphyria may manifest clinically in an acute or non-acute manner, and the signs and symptoms may be predominantly neurovisceral, psychiatric, cutaneous, or some combination of those. (medscape.com)
Biological1
- In order to learn the influence a sequence has on phenotype, or to discover its biological function, researchers can engineer a change or disrupt the DNA. (wikipedia.org)
Gene of interest1
- This might entail 'knocking in' recombinase sites (such as lox or frt sites) that will cause a deletion at the gene of interest when a specific recombinase (such as CRE, FLP) is induced. (wikipedia.org)
Interaction2
- As a result a specific signal is produced, indicating an interaction has taken place. (genomicglossaries.com)
- This phenotype is associated with interaction of E2 with the Mitotic Checkpoint Complex (MCC) proteins Cdc20, MAD2 and BUBR1. (docksci.com)
Cells7
- NFAT3 is rarely expressed in T cells, and the mechanisms and significance of the specific NFAT3 downregulation in T cells have been unknown. (aai.org)
- NFAT3 downregulation in Jurkat cells using RNA interference technology augmented IL-2 expression, whereas a knockdown of NFAT1, NFAT2, and NFAT4 suppressed it. (aai.org)
- However, rather than actively modifying the environment, multiple mechanisms guide the evolution of tumor cells through the selection of subclones with the most adaptive phenotype by environmental factors ( 1 ). (spandidos-publications.com)
- Meanwhile, fluorescence microscopy provides highly sensitive and specific imaging for living cells. (pressbrand.net)
- Dynamic phenotypes at the single-cell level can better differentiate cells' response to treatment than population-level averages, which is clinically relevant, such as in cancer therapy. (pressbrand.net)
- Microbial cells offer important advantages for ALE studies: (a) most microbial cells have simple nutrient requirements, (b) they can be easily cultivated in the laboratory and (c) microbial cells generally grow very fast and can be cultivated for several hundred generations within several weeks or months (with typical specific growth rates of microbial cells in the range of μ = 0.05 to 1.0 h -1 ). (biomedcentral.com)
- In this study, we explored the contribution of c-Jun N-terminal kinases (JNK) signaling to the mesenchymal phenotypes and the aberrant motile capacity of MDR cells utilizing a well characterized MDR cell line KB/VCR, which is established from KB human epidermoid carcinoma cells by vincristine (VCR), and its parental cell line KB. (biomedcentral.com)
Antibodies1
- Due to their small number of endogenous fluorescent molecules, fluorescent-labeled probes and antibodies can reveal the location of specific molecules in the cell. (pressbrand.net)
Transcription1
- As a largest plant-specific transcription factor family, NAC domain proteins play an important role in plant development and regulation of abiotic stress tolerance. (frontiersin.org)
Expression2
- An increased expression of RARß has been associated with improved breast cancer-specific survival. (bvsalud.org)
- In complementary experiments the overexpression of Buffy in the developing eye suppressed the mild rough eye phenotype that results from Gal4 expression and from alpha-synuclein expression. (sdbonline.org)
Mechanisms1
- Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
Basis1
- Exhaled aldehydes have been consistently demonstrated to be enriched in EAC patients 5 , 6 , and could form the basis of a non-invasive, primary care-based, triage test for patients with non-specific upper gastrointestinal symptoms. (nature.com)
Differences1
- Over the last two decades important insights into nutrient and stress metabolism of relevant model species were acquired, whereas some other aspects such as niche-specific differences of non-conventional cell factories are not completely understood. (biomedcentral.com)
Cell2
- However, intensity in PC and DIC images is not a quantification of phase delay, and low image contrast can make automatic image segmentation difficult at high cell densities. (pressbrand.net)
- Moreover, results from bulk transcriptome sequencing (RNA-seq) and SCT experiments revealed distinct transcriptomic signatures representing cell injury and stress in primary RPTE in contrast to the uncultured, freshly dissociated PT from human kidney. (simonsfoundation.org)
Find1
- Find support for a specific problem in the support section of our website. (mdpi.com)
Disorders1
- Data table showing topics related to specific cancers and associated disorders. (cancerindex.org)
Similar1
- Our results showed that IRV delivered by dMNP alone or in combination with IPV induced similar levels of RV-specific IgG and neutralizing antibody. (cdc.gov)
Data1
- 4 ], even though available data on specific Wolbachia -mosquitoes associations have considerably increased in the past decades [ 5 ]. (biomedcentral.com)
Article1
- Referenced article focusing on the relationship between phenotype and genotype. (cancerindex.org)
Higher2
- In contrast to D. melanogaster , both higher levels of H3K4me3, resulting from inactivation of the major H3K4me3 methyltransferase complex, and lower levels of H3K4me3, upon suppression of demethylases, are associated with longevity in C. elegans [ 15 , 16 ]. (aging-us.com)
- by contrast, subjects in a higher quartile of TG levels show lower lumbar BMD values in the pre-menopausal women. (who.int)
Ability1
- Analysis revealed that Buffy acted to rescue the associated loss of locomotor ability observed in the alpha-synuclein-induced model of PD, while Buffy RNA interference resulted in an enhanced alpha-synuclein-induced loss of climbing ability. (sdbonline.org)
Brain1
- Contrast-enhanced magnetic resonance imaging (MRI) is used to screen for NF2 of the brain and spine. (medscape.com)
Growth1
- In contrast, R62D reduced neurite length and impaired growth-cone filopodia formation. (jneurosci.org)
Domain1
- Notably, SAP30 formed a homodimer with 1 subunit binding to SIN3A and another subunit recruiting MLL1 through specific Phe186/200 residues within its transactivation domain. (bvsalud.org)