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AssaysFPIADetectionTime-resolvedAnalyzerELISAMicroscopyEnzyme activityAntibodyImportant biomoleculesFluorescentAnalyticalMethodQuantitativePlateLifetimeDyesTracerDeviceProbeMembraneProteinsEmitting speciesEfficacyTechniquesProteinAnalysisHighTestPublicationSmallFindDrugsLightProcessTags

Assays8

• The OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals recommends the use of four serological tests to rule out brucellosis in international animal trade: the buffered Brucella antigen tests (rose bengal test and buffered plate agglutination test), the complement fixation test (CFT), the enzyme-linked immunosorbent assays (ELISA) and fluorescence polarization assay (FPA). (journalveterinariya.ru)
• FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays. (stratech.co.uk)
• Beckman Coulter Life Sciences' Valita Titer assays range are rapid, high throughput IgG quantification assays which use fluorescence polarization (FP) for detection. (mybeckman.ca)
• The assays rely on interactions between a fluorescently labeled IgG Fc-specific probe and the Fc of an IgG. (mybeckman.ca)
• This generic type of device includes immunoassays and chromatographic assays for tacrolimus. (medicregister.com)
• 1514 Eisenhower Place Ann Arbor, MI 48108-3284, USA Arbor Assays builds the highest quality detection and immunoassay products for clinically important biomolecules. (contractresearchmap.com)
• 6859 Quality Way Portage, MI 49002 Eurofins AvTech Labs offers a range of analytical and bioanalytical services including in vitro ADME assays, in vivo PK, bioanalysis, immunoassay development and biomarker ID and GMP method development. (contractresearchmap.com)
• Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)

FPIA7

• The method is a fluorescence polarization immunoassay (FPIA) from Abbott Diagnostics. (cdc.gov)
• The specific monoclonal antibody and the fluoresceinated SAH analog tracer constitute the FPIA detection system. (cdc.gov)
• Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. (wikipedia.org)
• FPIA quantifies the change in fluorescence polarization of reaction mixtures of fluorescent-labelled tracer, sample antigen, and defined antibody. (wikipedia.org)
• A conventional FPIA follows the procedure below: A specific quantity of sample is added to reaction buffer. (wikipedia.org)
• Here, we demonstrate that the concentration of small therapeutics can be determined in whole blood within paper-like membranes using Fluorescence Polarization Immunoassay (FPIA). (hes-so.ch)
• The fluorescence polarization immunoassay (FPIA) developed by Abbott Laboratories (TDx system) for the dedication of cyclosporine in plasma was evaluated utilizing entire blood because the analytical pattern. (diastrip.com)

Detection6

• Immunoassays are used in most screening processes because they allow for large-scale screening and quick detection (Reference Armbruster, Schwarzhoff, and HubsterArmbruster 1993). (icg-12.org)
• Short- and long-range FRET co-functionalized fluorescence quenching collapsar probe regulates amplified and accelerated detection of Salmonella. (translationandethics.com)
• AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies. (stratech.co.uk)
• Multiplexed Detection of Secreted Cytokines at near-Molecular Resolution Elucidates Macrophage Polarization Heterogeneity. (uci.edu)
• It has a strong blue fluorescence and can be used to create blue fluorescent bioconjugates or labeling synthetic peptides for high-throughput detection. (adipogen.com)
• In both fluorescence NTA and EVQuant, adding 0.01% SDS maintained uEV integrity and increased aquaporin-2 detection. (exosome-rna.com)

Time-resolved4

• Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). (moleculardevices.com)
• Our multi-mode plate readers include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), HTRF, and AlphaScreen. (moleculardevices.com)
• Here the PHERAstar ® FS HTS microplate reader is used in a time-resolved fluorescence assay to study the loss of membrane integrity. (bmglabtech.com)
• Urine particles were quantified in whole urine using nanoparticle tracking analysis (NTA), time-resolved fluorescence immunoassay (TR-FIA), and EVQuant, a novel method quantifying particles via gel immobilization. (exosome-rna.com)

Analyzer4

• Plasma total homocysteine concentrations are calculated by the Abbott AxSym Immunoassay Analyzer using a machine-stored calibration curve. (cdc.gov)
• The change in polarization is proportional to the amount of antigen in sample, and is measured by a fluorescence polarization analyzer. (wikipedia.org)
• The solution is evaluated in a fluorescence polarization analyzer to gather a baseline measurement. (wikipedia.org)
• The solution is evaluated again by the fluorescence polarization analyzer. (wikipedia.org)

ELISA1

• ELISA Radio immunoassay FRET Magnetic immunoassay Fluorescence Immunoscreening Lateral flow test Cloned enzyme donor immunoassay Surround optical fiber immunoassay Plate reader Nielsen K, Lin M, Gall D, Jolley M (September 2000). (wikipedia.org)

Microscopy3

• The reagents are used as probes, stains, markers, NIR-labels, pH-sensors, ion indicators, chelators and in other applications (e.g. analytical biochemistry, immunoassays and microscopy) by life science researchers in academia, biotechnology and the diagnostics & pharmaceutical industry. (adipogen.com)
• This dye is commonly used for quantitating double-stranded DNA (dsDNA) of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry. (adipogen.com)
• Can be used to monitor cells by flow cytometry or fluorescence microscopy. (adipogen.com)

Enzyme activity1

• We build robust immunoassay and enzyme activity kits to quantitate biomolecules in biological matrices, provide custom assay testing services, and offer world-class customer support. (contractresearchmap.com)

Antibody4

• Fluorescence polarization immunoassays employ a fluorophore bound antigen that when bound to the antibody of interest, will increase fluorescence polarization. (wikipedia.org)
• Free tracer in solution has a lower fluorescence polarization than antibody-bound tracer with slower Brownian motion. (wikipedia.org)
• The tracer and the specific antigen will compete to bind to the antibody and if the antigen is low in concentration, more tracer will be bound to the antibody resulting in a higher fluorescence polarization and vice versa. (wikipedia.org)
• Purified mAbs are >90% specific antibody. (antibodiesinc.com)

Important biomolecules1

• The aim of this course is to apply the basic knowledge gained in Biochemistry (CHEM 361) course to specific metabolic reactions and certain physiologically important biomolecules. (uaeu.ac.ae)

Fluorescent1

• When a fluorescent-labelled molecule (tracer) binds to another molecule the rotational motion will change, resulting in an altered intensity of plane-polarized light, which results in altered fluorescence polarization. (wikipedia.org)

Analytical1

• Analytical analysis of TDx cyclosporine and metabolites fluorescence polarization immunoassay and comparability with the Cyclo-Trac RIA equipment. (diastrip.com)

Method2

• Cloned enzyme donor immunoassay (CEDIA), enzyme-multiplied immunoassay method (EMIT), fluorescence polarization immunoassay, immunoturbidimetric assay, and radioimmunoassay are all immunoassay techniques. (icg-12.org)
• However, in order to use this method to investigate SH2 binding sites on a specific target in cell lysate, additional procedures such as pull-down or immunoprecipitation which consume large amounts of sample are required. (biomedcentral.com)

Quantitative2

• From 1925 to 1926 Francis Perrin detailed a quantitative theory for fluorescence polarization in multiple significant publications which remain relevant to this day. (wikipedia.org)
• Phasor Analysis of Fluorescence Lifetime Enables Quantitative Multiplexed Molecular Imaging of Three Probes. (uci.edu)

Plate1

• Valita Titer is among the industry's fastest IgG tests and is compatible with all plate readers with a Fluorescence Polarization module. (mybeckman.se)

Lifetime1

• It shows a high extinction coefficient, polarity-dependent fluorescence and short excited-state lifetime. (adipogen.com)

Dyes1

• Tissue imaging allowed us to reconstruct the injured area spatially in 3D after staining it with specific nuclear dyes and to determine TBI resulting in cell death. (hes-so.ch)

Tracer1

• The fluorescence polarization value for the tracer containing solution is compared to the baseline and magnitude of difference is proportional to quantity of target analyte in sample. (wikipedia.org)

Device1

• A device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds, usually by electrical, thermal or optical signals. (genomicglossaries.com)

Probe1

• Trigging isothermal circular amplification-based tuning of rigorous fluorescence quenching into complete restoration on a multivalent aptamer probe enables ultrasensitive dete ction of Salmonella. (translationandethics.com)

Membrane3

• In this assay, target cells are loaded with fluorescence enhancing ligand, BATDA, which penetrates the cell membrane quickly and is hydrolyzed to form a hydrophilic ligand (TDA) that is released from cells only after cytolysis (Figure 1). (bmglabtech.com)
• These calcium channels mediate the influx of calcium ions into a cell upon membrane polarization. (antibodiesinc.com)
• Variations in membrane water content cause blue shifts in the laurdan emission spectrum, which are quantified by calculating the generalized polarization (GP). (adipogen.com)

Proteins3

• Multiplexed Immunoassay Using Quantum Dots to Monitor Proteins Secreted from Single Cells at Near-Single Molecule Resolution. (uci.edu)
• The fluorescence of this environment-sensitive dye is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins. (adipogen.com)
• Develop a strategy to purify specific proteins from bio-samples. (uaeu.ac.ae)

Emitting species1

• Observing that polarization increased with viscosity of the solvent and the size of the dye molecule, but decreased with an increase in temperature, he deduced that polarization increased with a decrease in mobility of the emitting species. (wikipedia.org)

Efficacy1

• We examined factors associated with PCP beliefs about screening efficacy and uncertainty and whether beliefs were associated with prostate specific-antigen (PSA) test use. (cdc.gov)

Techniques1

• The competitive homogenous characteristic allowed for the fluorescence polarization immunoassay to be automated much easier than other immunoassay techniques such as radioimmunoassays or enzyme-linked immunoassays. (wikipedia.org)

Protein1

• Framework nucleic acid-wrapped protein-inorganic hybrid nanoflowers with three-stage amplified fluorescence polarization for terminal deoxynucleotidyl transferase activity biose nsing. (translationandethics.com)

Analysis1

• Individuals together with SLE as well as PM/DM and lymphopenia might therefore make use of expose viral weight follow-up as well as cautious physical exam.Currently terahertz spectroscopy can be a well-established technique and recent moves along throughout technologies indicated that this specific new technique is helpful for both basic analysis and commercial applications. (kspinhibitors.com)

High1

• Has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. (adipogen.com)

Test1

• A very sensitive screening approach should be used first, followed by a highly specific confirmatory test for samples that are found to be positive. (icg-12.org)

Publication1

• Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. (uci.edu)

Small1

• Biotinylation is rapid, specific and is unlikely to perturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). (genomicglossaries.com)

Find1

• Find support for a specific problem in the support section of our website. (mdpi.com)

Drugs1

• The cells from eutopic endometrium and from ectopic endometriotic lesions can be used as the model for studying of the etiology and pathogenesis of endometriosis and for testing new drugs for this specific group of patients. (endometriosi.it)

Light1

• The rotation of the molecules between absorption and emission of the photon has the effect of "twisting" the polarization of the light. (mybeckman.ca)

Process1

• Generally, the conduction process (R) and polarization behavior (C) become dominant in sensing mechanism. (plasignaling.com)

Tags1

• In an oligonucleotide-tagged multiplex (OTM) assay, a mixture of SH2 domains with domain-specific DNA tags are incubated with a sample spot allowing for competitive binding (single sample per well) [ 8 ]. (biomedcentral.com)