Species conserved sequence. Medical search. Frequent questions

PB2 genes (viral ribonucleoproteins [RNPs]) and are most- encoded protein sequences have not been extensively ly located in the functional domains related to RNP-RNP reported. (cdc.gov)
Conserved sequences outside of known genes are likely to be gene regulatory elements. (sciencedaily.com)
Because regulatory elements are typically located near the genes they govern, the researchers assigned each conserved element to the closest gene. (sciencedaily.com)
In the first period of evolutionary innovation, in addition to changes affecting developmental genes, the study found a dramatic enrichment in conserved elements near genes for proteins known as "transcription factors," which bind to DNA and regulate whole groups of other genes. (sciencedaily.com)
For pathogenic bacteria sequences of genes, 16S rRNA, or entire genomes are popular choices of organizational structure. (news-medical.net)
Because microorganisms such as bacteria can undergo horizontal gene transfer, genes that are universally conserved across all bacteria are used as markers to ensure accuracy in the alignment. (news-medical.net)
The similarities and differences in the genes or genomes are then used to infer how closely related species are based on whether regions have changed, as well as how likely specific regions are to change. (news-medical.net)
For example, critical genes used for specific cell functions are less likely to change than unused sections of the genome, and therefore any changes in critical genes between two species are likely to indicate more distant relatedness. (news-medical.net)
This, in turn, also revealed that many genes present in the non-pathogenic species had been lost from the pathogenic species, presumably influencing their evolution. (news-medical.net)
Detailed studies of Leptospira proteins and genes that influence pathogenicity make more sense when placed in a phylogenetic context, such as one study showing how genes gained and lost by pathogenic and intermediately pathogenic species since their split from non-infectious species provide increased understanding of how bacterial pathogens change and adapt in response to their hosts. (news-medical.net)
It pinpoints genes that are essential to life and highlights genomic signals that control gene function across many species. (genome.gov)
The genomes of almost all living creatures, both plants and animals, consist of DNA (deoxyribonucleic acid), the chemical chain that includes the genes that code for different proteins and the regulatory sequences that turn those genes on and off. (genome.gov)
These studies are uncovering new behavioral, neurological and developmental pathways and genes that are shared or related among species. (genome.gov)
A study discovered that about 60 percent of genes are conserved between fruit flies and humans, meaning that the two organisms appear to share a core set of genes. (genome.gov)
Wnt genes are highly conserved between vertebrate species sharing overall sequence identity and gene structure, and are slightly less conserved between vertebrates and invertebrates. (embl-heidelberg.de)
Performance of phylogenetic foot-printing depends on the evolutionary distance between given species and on the conservation level of individual genes. (biomedcentral.com)
Regions of the L. killicki ( L. tropica ) and L. major genes sequences were scanned using BLAST program and subsequently compared using appropriate bioinformatic tools Chromas and MEGA. (who.int)
Whole genome sequencing can also provide insights into the specific genes or mutations that may be linked to antibiotic resistance. (folkhalsomyndigheten.se)
Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)

Advances in high-throughput DNA sequencing and protein mass spectrometry has substantially increased the availability of protein sequences and whole genomes for comparison since the early 2000s. (wikipedia.org)
Now researchers combing through the DNA sequences of vertebrate genomes have identified three distinct periods of evolutionary innovation that accompanied this remarkable diversification. (sciencedaily.com)
By comparing the genomes of species whose evolutionary lineages diverged at different times in the past, researchers can see when in evolutionary history a particular conserved sequence first appeared. (sciencedaily.com)
Alignment of HACNS4 to orthologous sequences from nonhuman genomes. (yale.edu)
During the past decade, two major developments-the emergence of massively parallel, ultra-cheap DNA sequencing technologies and the use of these technologies as digital readouts for functional genomic assays-have led to a profusion of data describing various features of genomes, epigenomes, and transcriptomes 1 , 2 . (biorxiv.org)
Results The 21 CSSD-badnaviral genomes and RT-RNase H sequences shared 70-100% and 72-100% identity, respectively. (figshare.com)
Methods of this type assume that regulatory regions are highly conserved in cross-genomic comparison, and conserved segments can be extracted from evolutionary related genomes. (biomedcentral.com)
This is an actively progressing area, as more and more sequenced genomes appear. (biomedcentral.com)

Each candidate enhancer sequence is PCR amplified from genomic DNA, cloned into an expression vector upstream of a minimal promoter driving expression of a reporter gene (e.g., lacZ) and the resulting construct injected into single cell mouse embryos. (yale.edu)
2018). Comparative and phylogenetic analysis of a novel family of Enterobacteriaceae- - associated genomic islands that share a conserved excision/integration module. (news-medical.net)
The resultant 14, and seven previously known, full-length badnaviral genomic and RT-RNase H sequences were analyzed by pairwise distance analysis to resolve species relationships, and by Maximum likelihood (ML) to reconstruct phylogenetic relationships. (figshare.com)
The potential application of our method includes annotation of new genomic sequences and motif discovery. (biomedcentral.com)
Therefore, to address the growing volumes of available genomic sequence, a number of algorithms that identify putative cis-regulatory modules and transcription factor binding sites using evolutionary comparisons, whole-genome data, and known descriptions of transcription factor binding sites, have been successfully developed. (biomedcentral.com)
Transposable elements (TEs) are mobile genetic sequences that comprise around 50% of our genomic DNA. (lu.se)

In evolutionary biology, conserved sequences are identical or similar sequences in nucleic acids (DNA and RNA) or proteins across species (orthologous sequences), or within a genome (paralogous sequences), or between donor and receptor taxa (xenologous sequences). (wikipedia.org)
Conserved proteins undergo fewer amino acid replacements, or are more likely to substitute amino acids with similar biochemical properties. (wikipedia.org)
As with proteins, nucleic acids that are important for the structure and function of non-coding RNA (ncRNA) can also be conserved. (wikipedia.org)
The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level. (rndsystems.com)
They encode proteins of ~350-400 residues in length, with orthologues identified in several, mostly vertebrate, species. (embl-heidelberg.de)
Very little is known about the structure of Wnts as they are notoriously insoluble, but they share the following features characteristics of secretory proteins: a signal peptide, several potential N-glycosylation sites and 22 conserved cysteines [ ( PUBMED:9891778 ) ] that are probably involved in disulphide bonds. (embl-heidelberg.de)
A superfamily of proteins that share a highly conserved MADS domain sequence motif. (bvsalud.org)
Many MADS domain proteins have been found in species from all eukaryotic kingdoms. (bvsalud.org)
This macromolecular assembly comprises more than 20 conserved proteins that form a series of ring-like structures and a needle-shaped protrusion, which allows the transport of proteins into a host cell. (lu.se)

With the advancement of next-generation sequencing and mass spectrometry, there is a growing need for the ability to merge biological features in order to study a system as a whole. (rsc.org)
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Next-Generation Sequencing in Medicine, available on its website in hardcover and ebook formats. (cshlpress.com)
The Public Health Agency's Next Generation Sequencing (NGS) platform performs massive parallel sequencing of DNA and RNA. (folkhalsomyndigheten.se)

Comparative genomics is a field of biological research in which researchers use a variety of tools to compare the complete genome sequences of different species. (genome.gov)
From the 142 confirmed cases, we performed unbiased (Illumina) and SUDV-amplicon-specific (Minion) high-throughput sequencing to obtain 120 SUDV genome-and coding-complete sequences, representing 95.4% (104/109) of SVD-confirmed individuals within a sequence-able range (Ct 30) and 10 genome sequences outside of this range and 6 duplicate genome sequences. (cdc.gov)

To this end, we have focused on identifying noncoding sequences highly conserved in nonhuman vertebrate species that display high rates of nucleotide substitution on the human lineage, which may indicate positive selection for new regulatory functions. (yale.edu)

We also demonstrate that self-propagating active genetic elements (CopyCat elements) can efficiently delete and replace the L2-CRM with orthologous sequences from other divergent fly species. (elifesciences.org)
Here, we model the changes in the binding sites by taking information from orthologous regions from different species and use evolutionary models namely, the Felsenstein 1981(F81) and Hasegawa-Kishino-Yano 1985 (HKY85) models of DNA evolution,to study the selective pressures that could be acting and driving the observed changes in the TFBSs. (imsc.res.in)

Human-specific substitutions at sites conserved from chimpanzee to chicken are expected to be uncommon events, and were consequently given more weight than substitutions at less conserved sites. (yale.edu)

avian strains were identified as most highly conserved. (cdc.gov)
Consequent to this rapid evolution, recently reported strains are indicating cross identification within the previously defined four distinct geographical clades.We propose a rapid and specific colony PCR-based clade identification system (CLaID) that uses unique DNA sequence junctions conserved in clade-specific manner for the classification of newly found strains of C.auris. (imsc.res.in)
The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)
Although the gene sequences were well conserved among different strains, their chromosome structures differed drastically. (wellnessresources.com)
Specificity of the primers was verified using the MCC member species, 11 non-MCC RGM species, 3 slow-growing mycobacterial (SGM) species (two strains each), and 19 field isolates, including 18 MCC isolates (from in-use MWF) and one non-MCC isolate (from reverse osmosis water). (cdc.gov)
The Mubende variant glycoprotein amino acid sequences exhibited 99.7% similarity altogether and a maximum of 96.1% glycoprotein similarity compared to historical SUDV strains from 1976. (cdc.gov)

In dogs, pigs and numerous other species, variants of the CGRP-like calcitonin receptor-stimulating peptide (CRSP) are expressed in place of β-CGRP. (bachem.com)
A collaborative team led by Debra Eckert, Ph.D., research assistant professor of biochemistry, and Michael S. Kay, M.D., Ph.D., professor of biochemistry, have identified a peptide sequence that is conserved in all known species of Ebola and is thought to control the virus' entry into human cells. (genengnews.com)
Epitope mapping has identified that the antibody recognizes a sequence/structure within the peptide CQIRFRFDGQPINE. (cytoskeleton.com)
The peptide sequence is conserved in mammals, birds, and amphibians, giving the antibody broad species reactivity. (cytoskeleton.com)
This peptide sequence is also 100% conserved between human, mouse and rat homologs of APP, and the antibodies against this sequence cross-react with all three species. (antibodiesinc.com)
One of the peptide sequences used is within the beta-amyloid fragment and recognizes the extracellular amyloid plaques observed in Alzheimer patients' brains (see Cat. (antibodiesinc.com)
Three other peptide sequences used, however, are within the extracellular domain of APP outside of this domain, and are useful for identifying the APP protein itself, and various proteolytic fragments, rather than the beta-amyloid peptide and plaques. (antibodiesinc.com)

Comparison of the conserved sequences of the small subunit rDNA clearly established the close relationship of B. akodoni with other members of the geus. (usda.gov)
NTM are typically grouped on the basis of their growth rate (ie, rapid or slow) and then further subcategorized based upon genetic relatedness, most commonly determined by 16s rRNA sequencing, rpoB sequencing (sequencing of a highly conserved subunit of the RNA polymerase), or whole genome sequencing. (medscape.com)
In contrast to metagenomic detection of unknown pathogens which sequences all available genetic material, 16S sequencing specifically targets the small ribosomal subunit. (folkhalsomyndigheten.se)
In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

Altogether, our findings elucidate conserved cellular and molecular mechanisms through which miR-34/449 regulate motile ciliogenesis. (nature.com)
[ 3 ] Improved molecular diagnostics have led to the identification of a multitude of new species, making the taxonomy of NTM a dynamic field. (medscape.com)

A highly conserved sequence is one that has remained relatively unchanged far back up the phylogenetic tree, and hence far back in geological time. (wikipedia.org)
Examples of highly conserved sequences include the RNA components of ribosomes present in all domains of life, the homeobox sequences widespread amongst eukaryotes, and the tmRNA in bacteria. (wikipedia.org)
Highly conserved DNA sequences are thought to have functional value, although the role for many highly conserved non-coding DNA sequences is poorly understood. (wikipedia.org)
Within a sequence, amino acids that are important for folding, structural stability, or that form a binding site may be more highly conserved. (wikipedia.org)
HACNSs show a statistically significant excess of human-specific sequence changes (circled) at positions highly conserved in other species. (yale.edu)
Mycobacterium genus-specific primers derived from highly conserved sequences in the ITS region and the flanking 16S rRNA gene were used. (cdc.gov)
Host species identity and host evolutionary history were both highly predictive of moss microbiome composition, highlighting strong phylogenetic coherence in these microbial communities. (biomedcentral.com)
Sequence comparison between VDE from different species were made and particular interest was focused on four highly conserved histidines (H121,124,167,173) and their possible involvement in enzymatic activity. (lu.se)
The Mubende variant exhibited 96% amino acid similarity with historic SUDV sequences from the 1970s and a high degree of conservation throughout the outbreak, which was important for ongoing diagnostics and highly promising for future therapy development. (cdc.gov)
The 16S rRNA gene is highly conserved with certain variable regions. (folkhalsomyndigheten.se)
Highly conserved regions are less likely to mutate, therefore there is a high likelihood that the diagnostic test will remain accurate in the presence of variants. (who.int)

For contact tracing or inquiries related to antibiotic resistance, whole genome sequencing (WGS) of bacterial isolates is offered. (folkhalsomyndigheten.se)
It is a platform and database for the automated analysis of whole genome sequencing data. (folkhalsomyndigheten.se)

After 4 weeks of conversion, we performed global analyses of RNA and protein levels by RNA-sequencing and mass spectrometry. (lu.se)

Homology search tools may take an individual nucleic acid or protein sequence as input, or use statistical models generated from multiple sequence alignments of known related sequences. (wikipedia.org)
Evolutionary turnover may cause inconsistencies between sequence orthology and functional homology that substantially limit this type of analysis. (biorxiv.org)

Evolutionarily conserved untranslated regions facilitate the cloning of complete coding sequences of chondriogenes encoding NADH dehydrogenase subunits. (techscience.com)

The mutations that produce genetic defects in humans will almost certainly turn out to be in well-understood parts of the gene or its closely associated regulatory sequences. (blogspot.com)

In coding sequences, the nucleic acid and amino acid sequence may be conserved to different extents, as the degeneracy of the genetic code means that synonymous mutations in a coding sequence do not affect the amino acid sequence of its protein product. (wikipedia.org)
Simmonds P . SSE: a nucleotide and amino acid sequence analysis platform. (microbiologyresearch.org)

The enzymes harbour a tuberculosis necrotizing toxin (TNT) domain and are predominately present in pathogenic species. (nature.com)
In this work, we identify NADases on the surface of fungi and show that these enzymes are predominately found in pathogenic species. (nature.com)
For example, the relationship between Mycobacterium species tends to show pathogenic species sharing a close relationship with each other and non-pathogenic species clustering together distinctly from the pathogenic species. (news-medical.net)

Conserved sequences are sequences which persist in the genome despite such forces, and have slower rates of mutation than the background mutation rate. (wikipedia.org)
The extent to which a sequence is conserved can be affected by varying selection pressures, its robustness to mutation, population size and genetic drift. (wikipedia.org)
Mutation screening of all coding regions and flanking intron sequences of the RP1L1 gene were performed with DNA sequencing analysis in this case with OMD. (molvis.org)
Other sources of experimental information include X-ray diffraction, biotinylation, sequence mutation and deletion, solution binding and chemical cross-linking data. (lu.se)

Non-coding sequences important for gene regulation, such as the binding or recognition sites of ribosomes and transcription factors, may be conserved within a genome. (wikipedia.org)
This was not helpful since it included a huge number of sites and sequences that result from spurious (nonfunctional) binding of transcription factors or accidental transcription of random DNA sequences to make junk RNA [see What did the ENCODE Consortium say in 2012? ]. (blogspot.com)

In epidemiological contact tracing, this technology enables the mapping of infection spread and identification of sources of infection through the comparison of genetic sequences. (folkhalsomyndigheten.se)

For example, the promoter of a conserved gene or operon may also be conserved. (wikipedia.org)
In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

Conservation indicates that a sequence has been maintained by natural selection. (wikipedia.org)
The study of sequence conservation overlaps with the fields of genomics, proteomics, evolutionary biology, phylogenetics, bioinformatics and mathematics. (wikipedia.org)
Conservation can occur in coding and non-coding nucleic acid sequences. (wikipedia.org)
However, sequence conservation in ncRNAs is generally poor compared to protein-coding sequences, and base pairs that contribute to structure or function are often conserved instead. (wikipedia.org)
The second, more practical consideration is that evolutionary conservation provides a means to interpret the statistical and ultimately biological importance of human-specific sequence changes. (yale.edu)
In our approach, sites within CNSs were binned based on their degree of sequence conservation in six nonhuman species: chimpanzee, rhesus macaque, mouse, rat, dog and chicken, and human-specific substitutions were identified by parsimony (Fig. 1A). (yale.edu)
Unfortunately, it is impractical, in terms of resources, to aim conservation support to all the species. (koneensaatio.fi)
Therefore, we need to prioritise a subset of all the species for conservation. (koneensaatio.fi)
However, proponents of these opposing camps have the same objective (i.e., effective conservation planning) and PD and FD both have properties desirable for prioritising species for conservation. (koneensaatio.fi)
From these taxon sets, three new metrics will be calculated which prioritises species sets for conservation with high degree of both PD and FD. (koneensaatio.fi)
This proposal is bold as it is the first attempt to prioritise species for conservation without compromising on either PD or FD and is guaranteed to always result in PD and FD which are better than it would have been if the taxa are chosen randomly or based on non-biological criteria. (koneensaatio.fi)
According to the world's biodiversity conservation organization, reptile species are most affected since their biological and ecological qualities are directly linked to climate. (techscience.com)
The proposed system is evaluated using various performance metrics and this framework can comfortably adapt to the decisions support to reintroduce the species for conservation in the future. (techscience.com)

These can be done on a large scale, such as the tree of life, or on a smaller scale, such as showing all the species of Mycobacterium bacteria. (news-medical.net)
Sequence variations between different bacteria provide a unique identity, and the analysis of these sequence enables taxonomic placement. (folkhalsomyndigheten.se)
The procedure's high sequence depth allows for these amplicons to be analysed separetely so different bacteria from the same sample can be identified. (folkhalsomyndigheten.se)

To identify putative regulatory sequences evolving rapidly on the human lineage, we developed a test statistic (in collaboration with Shyam Prabhakar of the Genome Institute of Singapore) for assessing the significance of human-specific sequence acceleration in conserved noncoding sequences (CNSs). (yale.edu)

In eukaryotes, the number and rough organization of chromosomes is well preserved within isolates of the same species. (wellnessresources.com)
The ML genome sequence tree resolved three well-supported clades, with ≥70% bootstrap, whereas, the RT-RNase H phylogeny was poorly resolved, however, both trees grouped CSSD isolates within one large clade, including the newly discovered Cacao red vein virus (CRVV) proposed species. (figshare.com)

In the last two decades, DNA barcoding, the sequencing of short species-specific amplicons, has considerably simplified community analyses (Hebert et al. (springer.com)
Sequencing of the approximately 250-bp-long ITS amplicons of the three MCC member species showed differences in 24 to 34 bases, thereby yielding variable deduced restriction maps. (cdc.gov)
The laboratory procedure is based on amplifying and sequencing four variable regions of the 16S rRNA gene with two amplicons. (folkhalsomyndigheten.se)

However, traditional Sanger sequencing-based DNA barcoding protocols can be prohibitively expensive and laborious when large community samples have to be processed. (springer.com)
Methods The DNA virome was determined from symptomatic cacao samples using Illumina-Hi Seq, and sequence accuracy was verified by Sanger sequencing. (figshare.com)

Conserved sequences are typically identified by bioinformatics approaches based on sequence alignment. (wikipedia.org)
Bioinformatics approaches outlined a low nucleotide identity of P. parmentieri and P. wasabiae with other species, while P. carotovorum subsp. (mdpi.com)

The sequences identified in the previous section of the work were used to design a combinations of 6 primers in 8 distinct regions of the target sequences for the amplification of a selection of reference DNAs belonging to the different Old World Leishmania species. (who.int)

Yet the relative importance of moss host species, and environmental factors, in structuring these microbial communities and their N 2 -fixing potential remains unclear. (biomedcentral.com)
Likewise, although moss microbial communities appear to be strongly influenced by host identity, it is unclear whether the differences in microbial community composition can be predicted from host phylogenetic relationships, the role of host species effects relative to geographic effects, and importantly, whether the composition of the microbial communities found in a given host species is stable across broad environmental gradients. (biomedcentral.com)
The basis of the GENSAM project is the joint national management of clinical microbial sequencing data. (folkhalsomyndigheten.se)

However, sequences of the more variable first internal transcribed spacer portion of the ribosomal DNA repeat support its differentiation from the other species of the genera. (usda.gov)
Method for rapid identification and differentiation of the species of the Mycobacterium chelonae complex based on 16S-23S rRNA gene internal transcribed spacer PCR-restriction analysis. (cdc.gov)
In order to minimize these health hazards, an effective and rapid assay for detection of MCC species and differentiation of MCC species from other species of rapidly growing mycobacteria (RGM) and from one another is warranted. (cdc.gov)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)

First, human-specific developmental features, as elaborations on the common theme of mammalian development, are likely to arise in part from changes in the conserved genetic architecture of the developmental regulatory machinery. (yale.edu)

The codon usage bias in some organisms may restrict the types of synonymous mutations in a sequence. (wikipedia.org)
Identifying DNA sequences that have been "conserved" - that is, preserved in many different organisms over millions of years - is an important step toward understanding the genome itself. (genome.gov)

We examined the changes in miRNA expression over the ontogeny of the German cockroach Blattella germanica , using 11 small RNA libraries prepared and sequenced in our laboratory, representing developmentally important stages. (biomedcentral.com)
First of all we proceeded to an alignment using the CLUSTAL W program to each Leishmania species and for the selected gene (cysteine protase b) (the alignment use published sequences and sequences which were sequenced in our laboratory). (who.int)

[ 4 ] Prior to 1992, M chelonae and M abscessus were considered to be the same species due to their nearly identical biochemical features. (medscape.com)

Many functional sequences are also modular, containing regions which may be subject to independent selection pressures, such as protein domains. (wikipedia.org)
The viral coding and non-coding sequences, genome organization, and predicted conserved protein domains (CPDs) were identified and characterized at the species level. (figshare.com)

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. (rndsystems.com)
In a second round of filtering that will follow, only those taxon sets will be kept which have identical sets of species between the filtered PD and FD. (koneensaatio.fi)

The first steps usually involve aligning the sequences of the various species, helping to identify sequences that are conserved or have remained the same, and sequences that have changed. (news-medical.net)

Short germ-band development is typical of basal insects such as locusts and cockroaches, whereas more derived species such as those of the genus Drosophila predominantly undergo long germ-band development [ 2 ]. (biomedcentral.com)

The amino acid sequences of CGRP peptides are well conserved among species, as illustrated in Scheme 1. (bachem.com)

As DNA sequencing technology becomes more powerful and less expensive, comparative genomics is finding wider applications in agriculture, biotechnology and zoology as a tool to tease apart the often subtle differences among animal species. (genome.gov)
allow proceeding to the in silico comparative genomics analysis of Leishmania DNAs and annotating the sequences identified. (who.int)

Towards this, we investigate the role of centromeric regions in these species across the four known clades and trace back the plausible evolutionary path taken by this species. (imsc.res.in)

2003 ). DNA barcodes can provide information on genetic variation within and between species, rapidly assign taxonomic status across divergent lineages (Hebert and Gregory 2005 ), and identify the prey composition of predators' gut contents (Agustí et al. (springer.com)

The systematic identification of conserved developmental regulatory elements has made it possible to study the impact of human-specific changes in regulatory DNAs on a genome-wide scale. (yale.edu)
We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. (biomedcentral.com)

Over many generations, nucleic acid sequences in the genome of an evolutionary lineage can gradually change over time due to random mutations and deletions. (wikipedia.org)
The nucleic acid sequence of a protein coding gene may also be conserved by other selective pressures. (wikipedia.org)
Nucleic acid sequences that cause secondary structure in the mRNA of a coding gene may be selected against, as some structures may negatively affect translation, or conserved where the mRNA also acts as a functional non-coding RNA. (wikipedia.org)
All nucleic acid in the clinical sample is sequenced and the resulting data is analysed by experienced bioinformaticians. (folkhalsomyndigheten.se)

We studied 26 boreal and tundra moss species across 24 sites in Alaska, USA, from 61 to 69° N. We used cultivation-independent approaches to characterize the variation in moss-associated bacterial communities as a function of host species identity and site characteristics. (biomedcentral.com)
16S sequencing is performed to determine which bacterial species are present in a clinical sample or isolate. (folkhalsomyndigheten.se)
The method uses an internal control sequence (spike) which is amplified and sequenced simultaneously with the sample to facilitate the assessment of the bacterial content and to detect amplification-inhibiting factors in the sample that may lead to a negative result. (folkhalsomyndigheten.se)

The Fp and Ip subunits are both very conserved with respect to composition and polypeptide sequence [1]. (lu.se)

The ENCODE Project has found that 80 per cent of the human genome sequence is linked to biological function. (blogspot.com)
The Dynamic approach is used to develop a knowledge-driven DSS using machine learning by applying an ecological and biological variable that characterizes the model and mitigation processes for species. (techscience.com)
In the third module, MkcDBGAS provides the analysis of differential splicing across multiple biological conditions when RNA-sequencing data is available. (bvsalud.org)

2014) points out that the purpose of the ENCODE project was to discover functional sequences in the human genome. (blogspot.com)
Many computational methods for gaining functional insights from sequence data are based on the simple, but powerful, observation that functionally important nucleotides tend to remain unchanged over evolutionary time, because mutations at these sites generally reduce fitness and are therefore eliminated by natural selection 7 - 15 . (biorxiv.org)

In this study, we undertook extensive genome-wide comparative analyses of twelve species that conform the Pectobacterium genus. (mdpi.com)
High-throughput sequencing (HTS), particularly DNA metabarcoding, enables community-wide analyses of diversity and interactions at unprecedented scales and at a fraction of the cost that was previously possible. (springer.com)

Ebola Reston virus (RESTV) is one of the six virus species of the Ebola virus in the family Filoviridae. (who.int)

These new regulatory elements are evolutionary innovations that have been passed on to all the descendants of the species in which they first arose," Haussler said. (sciencedaily.com)
Many conserved non-coding sequences (CNSs) function as cis-regulatory elements. (bvsalud.org)
However, predicting clade-wide cis-regulatory elements across several closely related species remains challenging. (bvsalud.org)

More generally, the relative importance of environment versus host species in mediating moss microbiome composition remains unresolved. (biomedcentral.com)

Data were compared with equivalent data for two long germ-band holometabolan species Drosophila melanogaster and Drosophila virilis , and the short germ-band holometabolan species Tribolium castaneum . (biomedcentral.com)
miRNA commonalities between B. germanica and T. castaneum , as opposed to those of the two Drosophila species studied, were assumed to be associated with the germ-band type, whereas those of T. castaneum and the Drosophila species, as opposed to B. germanica , were assumed to be associated with the mode of metamorphosis followed. (biomedcentral.com)
Members of the Wnt gene family are defined by their sequence similarity to mouse Wnt-1 and Wingless in Drosophila. (embl-heidelberg.de)

In contrast, short germ-band species start gastrulation with just a few segments, and then progressively add new ones from an undifferentiated growth zone situated at the posterior end of the embryo [ 1 ]. (biomedcentral.com)

Multiple sequence alignment entropy value between 0 and -0.4 for both the human and was performed with ClustalW version 1.83 (ftp://ftp. (cdc.gov)

For pathogenic microorganisms, this is typically done by sequencing the genome or other genetic information. (news-medical.net)
[ 8 ] Although typically susceptible to macrolides, which are a common backbone in NTM therapy, the resistance patterns of M chelonae species make treatment quite challenging. (medscape.com)

I am currently sequencing conserved elements of their genome to infer a phylogeny. (koneensaatio.fi)

Genes19

Genomes8

Genomic6

Proteins9

Next-Generation Sequencing3

Genome sequences2

Vertebrate1

Orthologous2

Less conserved1

Strains6

Peptide7

Subunit4

Molecular2

Highly11

Whole genome2

Mass spectrometry1

Homology2

Complete coding se1

Humans1

Amino acid seq2

Pathogenic species3

Mutation4

Transcription factors2

Genetic sequences1

Operon2

Conservation13

Bacteria3

Identify putative1

Isolates2

Amplicons3

Sanger2

Bioinformatics2

Target sequences1

Microbial3

Differentiation4

Mammalian1

Organisms2

Laboratory2

Abscessus1

Protein domains2

Identical2

Various species1

Undergo1

Among species1

Comparative genomics2

Clades1

Taxonomic1

Developmental2

Nucleic acid4

Bacterial3

Subunits1

Biological3

Functional2

Analyses2

Ebola1

Regulatory elements3

Host species1

Drosophila3

Segments1

Alignment1

Typically2

Infer1