Species cells in vitro. Medical search. Frequent questions

There is also a surprising lack of knowledge of the effects of fatty acids in the 'real world", in which normal cells in vivo are presented with mixtures of dietary fatty acids. (wits.ac.za)
The development of a protocol for the routine continuous in vitro culture of Plasmodium falciparum in 1976 1 released malaria researcher from the reliance on in vivo observations. (nature.com)
We show that the in vitro-cultured P. cynomolgi (from ex vivo or cryopreserved stocks) retain the key characteristics that these parasites share with P. vivax . (nature.com)
To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
Both in-vitro and in-vivo studies revealed that cold plasmas selectively kill cancer cells. (nature.com)
Cold atmospheric plasma (CAP), a highly reactive (partially) ionized physical state containing a dose controlled mixture of biologically-active agents has been shown to exert anticancer effects in vitro and in vivo ( 6 - 9 ). (iiarjournals.org)
Our novel blood brain barrier model allows direct cell-to-cell interactions, closely modeling the in vivo properties. (sigmaaldrich.com)
Our preclinical studies demonstrate that Triphala is effective in inhibiting the growth of human pancreatic cancer cells in both cellular and in vivo model. (biomedcentral.com)
CONCLUSION: YJZYD could attenuate reproductive endocrine disturbance and ovarian lesions in vivo by mediating steroidogenesis, energy metabolism, and cell apoptosis. (bvsalud.org)
It is the in vivo situation where blood cells are worth mentioning that many of the electron- suspended in their original medium. (who.int)

The reactive oxygen species (ROS) generation and inducible nitric oxide synthetase expression were also inhibited dose-dependently by lycopene. (nih.gov)
Reactive oxygen species (ROS) can be generated when organisms are exposed to a material causing cellular damage such as lipid peroxidation, H2O2 production and DNA damage. (cdc.gov)
It is shown that reactive oxygen species metabolism and oxidative stress responsive genes are deregulated. (nature.com)
We do know radiofrequency radiation increases the production of reactive oxygen species and leads to changes in intracellular enzymes, changes in gene expression, and nuclear DNA damage. (ewg.org)
These active agents, generated by focused electrical discharge, include positive and negative ions, electrons, excited atoms and molecules, free radicals such as singlet oxygen, superoxide, reactive oxygen and nitrogen species (ROS and RNS), photons and electromagnetic radiation ( 10 - 12 ). (iiarjournals.org)
Reactive oxygen species (ROS) are chemically reactive, oxygen-containing molecules. (sigmaaldrich.com)
There are a number of reactive nitrogen oxide species, such as N2O3 and ONOO-, that can alter critical cellular components under high local concentrations of NO. However, NO can also abate the oxidation chemistry mediated by reactive oxygen species such as H2O2 and O2- that occurs at physiological levels of NO. In addition to the antioxidant chemistry, NO protects against cell death mediated by H2O2, alkylhydroperoxides, and xanthine oxidase. (researchgate.net)
Triphala-mediated reduced cell survival correlated with induction of apoptosis, which was associated with reactive oxygen species (ROS) generation. (biomedcentral.com)
Reactive oxygen species (ROS), products of normal cell metabolism may cause damage to biological macromolecules leading to severe health threats when they are present in high concentrations. (degruyter.com)
In an in vitro model, mango and pyrogallol increased reactive oxygen species (ROS) generation and arrested cells in S phase. (oregonstate.edu)
The effects of EGCG on glucose oxidase (GO)-induced reactive oxygen species (ROS) production was determined using the ROS probe CM-H 2 DCFDA. (molvis.org)
The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. (who.int)
At therapeutic doses of metronidazole (4.98-24.86 μg/mL) significant inhibition of the production of reactive oxygen species was noted in both methods. (who.int)
The data suggest a scavenging mechanism of metronidazole on reactive oxygen species generated by PMN. (who.int)
phosphatidylserine (PS) externalisation and the generation of reactive oxygen species and nitrogen species. (bvsalud.org)
No statistically significant changes were observed in reactive oxygen and reactive nitrogen species generation. (bvsalud.org)
With intact cells, the to radiation killing compared with well formation of oxygen reactive intermediates oxygenated healthy cells [ 2,3,7-9 ]. (who.int)
The globally harmonized in the workplace are known as respiratory sensitizers, but system (GHS) for classification and labelling of chemicals this term is not used for workplace substances that aggra- ranks respiratory sensitization with other toxic effects of vate preexisting asthma or cause acute irritant-induced highest concern: carcinogenicity, germ cell mutagenicity asthma, also known as the reactive airways dysfunction and reproductive toxicity [4] . (cdc.gov)

Biological drug disovery including cell culture, assay development, animal model development, PK/PD model development (Pharmacokinetics & Pharmacodynamic) and mechanism of action (MOA) research. (genemedi.net)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
The comet assay was used to measure the DNA damage induced in vitro by nicotine in human leukocytes as the extent of DNA migration in the comet head area, tail length, percent DNA in the tail, and Olive tail moment. (who.int)
They then evaluated the in vitro cytotoxicity of these extracts against cancer cell lines using MTT assay. (naturalnews.com)
T cell proliferation assay results showed that antigenic activity was associated with proteins from HPLC fractions 4 through 6. (usda.gov)
Explore tumor metastasis employing various cell migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay and in vitro scratch assay. (sigmaaldrich.com)
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. (sigmaaldrich.com)
A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue. (sigmaaldrich.com)
Learn how to set up a T cell killing assay using Millicell ® Microwell plates. (sigmaaldrich.com)
Cell migration and invasion can be evaluated in vitro with widely-used membrane-based migration systems such as the Boyden Chamber assay. (sigmaaldrich.com)
Cell-based assay development for drug discovery can accelerate optimization of treatments for cancer and other angiogenesis-dependent diseases. (sigmaaldrich.com)
Growth-inhibitory effects of Triphala were evaluated in Capan-2, BxPC-3 and HPDE-6 cells by Sulphoradamine-B assay. (biomedcentral.com)
Apoptosis was determined by cell death assay and western blotting. (biomedcentral.com)
Cell metabolic activity was measured using the alamarBlue assay. (molvis.org)
Effects of EGCG on mitogen-activated protein kinase (MAPK) phosphorylation were determined by cell-based enzyme-linked immunosorbent assay (ELISA) and western blotting. (molvis.org)

1989. In vitro metabolism of the pro-carcinogen aflatoxin B1 by liver preparations of the calf, nurse shark, and clearnose skate. (mote.org)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)

The primary objective of this study was to verify whether a natural antioxidant, lycopene, could protect MSCs from ischemia-induced apoptosis in vitro. (nih.gov)
In this study, the effects of intracellular delivery of various concentrations of gallic acid (GA) as a semistable antioxidant, gallic acid-loaded mesoporous silica nanoparticles (MSNs-GA), and cellular uptake of nanoparticles into Caco-2 cells were investigated. (springer.com)
Aiyegoro OA, Okohy AI (2010) Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. (springer.com)
Above mentioned effects were significantly blocked when the cells were pretreated with an antioxidant N-acetylcysteine (NAC), suggesting the involvement of ROS generation. (biomedcentral.com)

We have recently recapitulated the segmentation clock, oscillatory gene expression during early development, from human and mouse pluripotent stem cells. (irbbarcelona.org)
Yamanaka's Nobel Prize-winning research paved the way for similar in vitro development from induced pluripotent stem cells (iPSCs). (wur.nl)
Their team focuses on using human induced pluripotent stem cells to grow human tissues inside pigs. (the-scientist.com)
Human pluripotent stem cells harbor the potential to provide an inexhaustible supply of donor cells or tissues or organs for transplantation," Wu wrote in an email. (the-scientist.com)
In 2007, a team at Kyoto University created pluripotent stem cells from adult human somatic cells (4). (the-scientist.com)
Researchers began to dream of a future in which a patients' own cells, perhaps from the blood or the skin, could be converted into these induced pluripotent stem cells and grown into whatever organ the patient needed. (the-scientist.com)
With advancements in stem cell technology, the possibility to generate astrocytes in vitro from human pluripotent stem cells (hPSCs), ultimately derived from patient cells, emerged. (lu.se)

Background/Aim: Cold atmospheric plasma (CAP) attenuates tumor cell proliferation and induces apoptosis in various cell lines. (iiarjournals.org)
They also reported that ELPA-treated cells showed signs of apoptosis (programmed cell death) and necrosis (death due to lack of blood supply), with cell cycle arrest in the G2/M phase. (naturalnews.com)
Based on these results, the researchers concluded that the cytotoxic activity of ELPA against cancer cells may be due to the synergistic action of flavonoids in it, which induces cell death by apoptosis and necrosis. (naturalnews.com)
Triphala-induced apoptosis was linked with phosphorylation of p53 at Ser-15 and ERK at Thr-202/Tyr-204 in Capan-2 cells. (biomedcentral.com)
Pretreatment of cells with pifithrin-α or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. (biomedcentral.com)
Similarly, Triphala induced apoptosis in another pancreatic cancer cell line BxPC-3 by activating ERK. (biomedcentral.com)
On the other hand, Triphala failed to induce apoptosis or activate ERK or p53 in normal human pancreatic ductal epithelial (HPDE-6) cells. (biomedcentral.com)
Reduced tumor-growth in Triphala fed mice was due to increased apoptosis in the tumors cells, which was associated with increased activation of p53 and ERK. (biomedcentral.com)
AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)

These assays may be used to quantify and to assess the health of mammalian cell lines, primary cells and stem cells. (sigmaaldrich.com)

Bovine leukemia virus (BLV), a deltaretrovirus, causes B-cell leukemia/lymphoma in cattle and is prevalent in herds globally. (cdc.gov)
Bovine leukosis (B-cell leukemia/lymphoma), first described in 1871 in Lithuania, was believed to be an infectious disease because it spread through herds of cattle. (cdc.gov)
This research will enhance our understanding of bovine host cell-mediated immune defense mechanisms and may facilitate development of subunit vaccines against neosporosis. (usda.gov)
Our objective was to determine whether this would be the case in another mammalian species, the bovine. (bioone.org)
To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)

Exposure of Capan-2 cells to the aqueous extract of Triphala for 24 h resulted in the significant decrease in the survival of cells in a dose-dependent manner with an IC50 of about 50 μg/ml. (biomedcentral.com)

The aim of the study was to analyse the effects of different CAP sources and application times on osteosarcoma (OS) cells and non-malignant fibroblast cell proliferation. (iiarjournals.org)
Cell proliferation was determined by viable cell count at different time points after treatment. (iiarjournals.org)
Conclusion: CAP treatment attenuates cell proliferation of OS cancer cells and fibroblasts in a treatment time-dependent manner, whereby U2-OS cells appeared more sensitive to CAP treatment as 3T3 fibroblasts after 10 sec of treatment. (iiarjournals.org)
Cell viability and proliferation assays can be effectively used to evaluate cell health. (sigmaaldrich.com)
Cell viability and proliferation rate are often assessed using dyes such as trypan blue or Calcein-AM, or with fluorescent readouts. (sigmaaldrich.com)
In the present study, we demonstrate that Triphala significantly inhibit the proliferation of Capan-2 and BxPC-3 human pancreatic cancer cells. (biomedcentral.com)
The objective of this study was to assess the underlying mechanisms of mango polyphenol decreased cell proliferation and tumor volume in ductal carcinoma in situ breast cancer. (oregonstate.edu)
These results suggest that mango polyphenols and their major microbial metabolite, pyrogallol, inhibit proliferation of breast cancer cells through ROS-dependent up-regulation of AMPK and down-regulation of the AKT/mTOR pathway. (oregonstate.edu)
In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)

Sperm cells are particularly susceptible to damage from free radicals that can be generated from radiofrequency radiation exposure, since they lack the protective antioxidants typical of other cells in the body. (ewg.org)
Changes to ovary cells have been associated with radiofrequency radiation exposure in laboratory rats. (ewg.org)
Prenatal exposure to radiofrequency radiation in laboratory rats was associated with follicle degeneration and structural changes of ovary cells that produce estrogen. (ewg.org)
Exposure to radiofrequency radiation from cell phones was associated with the death of the uterine lining in laboratory rats. (ewg.org)
exposure to 1 h of 900-MHz irradiation induced an increase in PS externalisation and an increase in the formation of F-actin stress fibres in MCF-7 cells. (bvsalud.org)

Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. (nature.com)
In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)

Additionally, IUCN Red List database shows that plant extinctions are dominated by insular species, and the extinction data are associated with lower competitive ability and anthropogenic factors (Gray 2018). (researchsquare.com)

Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
tizer drug, especially for hypoxic cells [ 9 ]. (who.int)

Taxus globosa S. cell lines: Initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production. (wikipedia.org)
Hu H, Nie L, Feng S, Suo J (2013a) Preparation, characterization and in vitro release study of gallic acid loaded silica nanoparticles for controlled release. (springer.com)
We have performed extensive phenotypic and functional characterization to confirm an astrocytic identity of the obtained cells. (lu.se)

Dr. Bernard Roelen of the Faculty of Veterinary Medicine, Utrecht University, explained what can be expected from production of sperm and oocytes in vitro, on the basis of the latest international scientific progress in mice. (wur.nl)
Embryonic stem cells (ESCs) from the 'inner cell mass' of an early embryo give rise to all future cells and tissue of an animal, including the primordial germ cells, which ultimately form the germ cells (sperm or oocytes). (wur.nl)

In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
In a cytogenicity study in mammalian cells, conducted according to OECD Test Guideline 473 and in compliance with GLP, the analogous substance dichloro(3-chloropropyl)methylsilane (CAS 7787-93-1) was negative in Chinese hamster fibroblast cells (Hüls AG, 1997, Reliability Score 1). (europa.eu)
Throughout the study, the incubation of the cells was conducted in a CO2 incubator controlled at 37+0.5°C, 5% of CO2 and nearly 100% relative humidity. (europa.eu)
The test substance was tested in L5178Y cells in presence and absence of metabolic activation in a study performed according to OECD 476. (europa.eu)
In this study, we examined the therapeutic potential of a cold plasma jet in cancer cell lines and tumours, focusing on selective tumour cell eradication capabilities and signalling pathway deregulation. (nature.com)
In a study published in the Journal of Medicinal Plants Research , Brazilian researchers investigated the cytotoxicity of various Passiflora species using colon cancer, ovarian cancer and leukemia cell lines. (naturalnews.com)
Of the 14 species included in the study, they found that the plant Passiflora alata , also known as the winged-stem passionflower, is rich in flavonoids with anti-cancer activity. (naturalnews.com)
For their study, the researchers used ethanol to obtain extracts from the leaves of 14 Passiflora species in a method called accelerated solvent extraction. (naturalnews.com)
They then conducted morphological analyses using light and fluorescence microscopy, and cell cycle and DNA fragmentation analyses using flow cytometry to study the mechanism of cell death induced by ELPA in HL-60 cells. (naturalnews.com)
3 In an in vitro study using canine cell cultures, Carprofen demonstrated selective inhibition of COX-2 versus COX-1. (drugs.com)
To study differences in time between species, my group uses in vitro systems. (irbbarcelona.org)
The objective of this study was to identify N. caninum antigens as vaccine candidates using CD4+ T cells established from N. caninum immunized and challenged cows. (usda.gov)
Cell migration and invasion kits are oftenn used in cancer research to study cell motility and invasiveness in response to chemoattractant gradients. (sigmaaldrich.com)
Angiogenesis assays can be used to study both the induction and inhibition of angiogenesis in in vitro cell models. (sigmaaldrich.com)
As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)
Today, Garry and her husband Dan, a transplant cardiologist, are pioneers in the field of interspecies chimera research, the study of organisms containing cells from two different species. (the-scientist.com)
In this study, we base our research on the plant metabolite pulchrol, a natural benzochromene which has been shown to possess antiparasitic activity against Trypanosoma and Leishmania species. (lu.se)

Bone marrow mesenchymal stem cells (MSCs) have been identified to have the potential to differentiate into multiple types of cells. (nih.gov)
Chung TH, Wu SH, Yao M, Lu CW, Lin YS, Hung Y, Mou CY, Chen YC, Huang DM (2007) The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles 3T3-L1 cells and human mesenchymal stem cells. (springer.com)

The current International Union for Conservation of Nature (IUCN) Red List categories and criteria are constructed to assess the threatened status of species or lower taxa on global or sub-global (national or regional) levels as a means of classifying the relative risk of extinction of taxon (Inceer and Aksu Kalmuk 2019). (researchsquare.com)

Carprofen has also been shown to inhibit the release of several prostaglandins in two inflammatory cell systems: rat polymorphonuclear leukocytes (PMN) and human rheumatoid synovial cells, indicating inhibition of acute (PMN system) and chronic (synovial cell system) inflammatory reactions. (drugs.com)

Elvira GM, Chandra S, Marco Vinicio RM, Wenyi W (2006) Catalytic inhibition of human DNA topoisomerase by phenolic compounds in Ardisia compressa extracts and their effect on human colon cancer cells. (springer.com)
When cells were treated with IL-1β and EGCG or hyperosmolarity and EGCG there was a dose-dependent reduction in release of these cytokines/chemokines, with significant inhibition observed at 3-30 µM. (molvis.org)

However, unlike other oncogenic retroviruses, deltaretroviruses have an additional region, tax (trans-activating region of the X gene), which has regulatory functions and is oncogenic to host cells. (cdc.gov)
We found that this period difference between species stems from slower biochemical reactions in human cells, including slower protein degradation and longer delays in gene expression processes. (irbbarcelona.org)
This contract focuses on gene therapy, which has the advantage over protein therapy to target specific cells for gene transduction leading to production or deduction of proteins precisely where therapy is needed. (sbir.gov)
For gene delivery, adeno-associated virus (AAV)-vectors are widely used gene delivery vectors for gene therapy due to features such as tissue tropism, potential of gene transfer to non-dividing cells, and long-term expression. (sbir.gov)
By engineering new delivery vehicles using novel biomaterials or delivery modalities, gene therapy can have enhanced stability and bioavailability, decreased immunogenicity, increased brain delivery, and improved cell-type targeting. (sbir.gov)
As scientific understanding of stem cells, gene editing, and organism development improved, Garry felt that her career path was clear. (the-scientist.com)

SiNWs were assessed using three different in vitro models (chemical, RAW 264.7 cells and rat alveolar macrophages) for ROS generation and possible toxicity. (cdc.gov)
Mtb is an intracellular pathogen able to survive indefinitely under unfavorable conditions inside primary host immune cells, preferably residing in human alveolar macrophages ( 5 , 6 ). (frontiersin.org)

CANCIDAS is the first of a new class of antifungal drugs (echinocandins) that inhibit the synthesis of (beta) (1,3)-D-glucan, an integral component of the fungal cell wall. (theodora.com)
Ibrexafungerp inhibits glucan synthase, an enzyme involved in the formation of 1,3-β-D-glucan, an essential component of the fungal cell wall. (centerwatch.com)

Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
In a chromosome aberration test in CHO-WBL cells, structural (with metabolic activation) and numerical (without metabolic activation) aberrations were reported after 3-hour treatment at concentrations up to cytotoxicity. (europa.eu)
Cell phone users have higher concentrations of testosterone and lower concentrations of luteinizing hormone that controls the production of sperm cells, compared to people who don't use cell phones. (ewg.org)
MSNs did not show significant toxicity in various concentrations (0-500 μg/ml) on Caco-2 cells. (springer.com)
For MSNs-GA, cell viability was reduced as a function of incubation time and different concentrations of nanoparticles. (springer.com)
Metronidazole selectively radiosensitizes of metronidazole (in a range of therapeutic hypoxic cells without influencing the ra- concentrations) on PMN-generated free diation response of normal well-oxygenated radicals during phagocytosis activity in cells [ 5,7-9 ]. (who.int)

In vitro genetic toxicity tests conducted according to OECD Test Guideline 473 and 490 will be conducted and added to the dossier when available. (europa.eu)
It is worth mentioning that many of the electron-affinic radiosensitizers of hypoxic cells also have the property of preferential or selective toxicity directed against hypoxic cells even in the absence of radiation [1,2]. (who.int)

The sperm cells of cell phone users were abnormally formed, compared to people who don't use cell phones. (ewg.org)
Many different types of cell, including sperm, bacteria and algae, propel themselves using whip-like appendages known as flagella. (cam.ac.uk)

The attenuation of metal/peroxide oxidative chemistry, as well as lipid peroxidation, appears to be the major chemical mechanisms by which NO may limit oxidative injury to mammalian cells. (researchgate.net)

Mycoplasma pneumoniae is one of the few Mycoplasma species that causes human disease. (medscape.com)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
Wireless devices like cell phones, Wi-Fi and other communication technologies release radiofrequency radiation that can harm the human body. (ewg.org)
Animals with human cells could provide donor organs or help us understand neuropsychiatric disorders. (the-scientist.com)
Other scientists, including Jun Wu, a stem cell biologist at the University of Texas Southwestern Medical Center, are also studying chimeras with the ultimate goal of one day being able to grow enough human organs to meet the enormous need for transplants, potentially saving hundreds of thousands of lives. (the-scientist.com)
Some researchers are attempting to use stem cells to bioengineer human organs in the lab in vitro , rather than inside another species (5). (the-scientist.com)
We think that the developmental cues that exist in the pig will help to guide the human cells inside the porcine embryo. (the-scientist.com)
To determine the anti-inflammatory and anti-oxidant effects of epigallocatechin gallate (EGCG), the major polyphenol component of green tea, in human corneal epithelial cells (HCEpiC). (molvis.org)
The human brain and human astrocytes have unique structures and functions that are not present in other animal species. (lu.se)
Studies of fundamental astrocyte biology in humans and their role in neurological disease have been hindered by shortage of native human astrocytes for research purposes and inadequate animal and cell models. (lu.se)

reviews the possibilities available through in vitro culture for this species, including their constraints (contamination, hyperhydricity and browning), explant types and media and plant growth regulators required for budding and subculture. (plantae.org)
Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax . (nature.com)
Affintiy&bioactivity validated by ELISA, cell culture validated. (genemedi.net)
In vitro culture presents the additional advantages of a reduced growth chamber space and non-daily maintenance. (frontiersin.org)
CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
In the cell culture, they can bind Ulex europaeus lectin and acetylated low-density lipoprotein (LDL) [ 2 ]. (hindawi.com)
Each culture was prepared by seeding with 1.4 × 10E6 CHO cells per 75 cm2 flask in McCoy's complete media and incubated at 37 ± 1°C in vented flasks in a humidified atmosphere of 5 ± 1% CO2 for approximately 24 hours prior to the initiation of treatment. (europa.eu)
2012). In this context, in vitro culture techniques have permitted an increasingly important viable system for rapid, true-to-type mass multiplication as well as conservation of threatened endemic and rare species (Piovan et al. (researchsquare.com)

In vitro studies demonstrate that exogenous recombinant Gal3 is internalised by neuronal cell lines and primary neurons where it interacts with endogenous αSyn fibrils. (lu.se)

The remarkable morphological and biological similarities between these two parasite species are now known to extend to their genetic make-up 3 , 4 . (nature.com)
Dr. Henri Woelders, of Wageningen Livestock Research (WLR) and the Centre for Genetic Resources, the Netherlands (CGN) presented on the potential of cryopreservation and use of various types of germplasm in pigs and chicken, focussing on embryos, gonads, primordial germ cells and semen, and including some highlights from the EU Horizon 2020 IMAGE project (LINK). (wur.nl)
In line with our in vitro studies, under these conditions, genetic deletion of GAL3 leads to increased intracellular αSyn accumulation within dopaminergic neurons and remarkably preserved dopaminergic integrity and motor function. (lu.se)

The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
However, advances in stem cell research in the 2000s revolutionized the field, opening up new possibilities and new applications for multispecies organism research. (the-scientist.com)
Stem Cell Reports. (lu.se)

In mouse embryos, cells seem prepatterned to become certain cell lineage because the first cleavage plane has been related with further embryonic-abembryonic axis at the blastocyst stage. (bioone.org)
Surprisingly, we found that there is a significant percentage of the embryos (∼60%) with labeled and nonlabeled cells randomly distributed and intermingled. (bioone.org)
Utrecht can do ovum pick-up from mares and collaborate with Avantea, Italy for in vitro production and cryopreservation of embryos. (wur.nl)

Metronidazole selectively radiosensitizes hypoxic cells without influencing the radiation response of normal well-oxygenated cells [5,7-9]. (who.int)

If the neutrophil count is less than or equal to 1000 cells/mm 3 , terbinafine tablets should be discontinued. (nih.gov)

The overall loss of biodiversity as well as the number of threatened plant species in the world has increased in recent years due to several anthropogenic pressures and/or environmental factors (Corral et al. (researchsquare.com)

Mycoplasma organisms cause infection primarily as extracellular parasites, attaching to the surface of ciliated and nonciliated epithelial cells of the respiratory and genital tracts. (medscape.com)
Following attachment, mycoplasmal organisms may cause direct cytotoxic damage to epithelial cells because of hydrogen peroxide generation or cytolysis via an inflammatory response mediated by mononuclear cells or antigen-antibody reactions. (medscape.com)

We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)

Animal studies also show radiofrequency radiation can interfere with estrogen production, damage the lining of the uterus and developing egg cells, and cause changes to the heart of the developing fetus. (ewg.org)
Ibrexafungerp has concentration-dependent fungicidal activity against Candida species as measured by time kill studies. (centerwatch.com)
Some species, like P. foetida or stinking passionflower and P. serrato-digitata , can also be used to relieve stomach problems , particularly stomach ulcers, according to studies. (naturalnews.com)
These studies indicate that antigen-specific T cells may be used to identify N. caninum antigens that may stimulate a protective immunity. (usda.gov)
Several studies have recently shown that features such as its high infectivity, slow growth, and complex cell-wall structure, make M. tuberculosis a major challenge to be faced, since the specific mechanisms that reinforce the high virulence of Mtb remain largely unknown ( 4 , 7 ). (frontiersin.org)

Few researchers focus their work on this species, the team leader on Taxus globosa S. is perhaps that of Barradas at Veracruz Institute of Technology (Instituto Tecnológico de Veracruz). (wikipedia.org)
The researchers also determined the IC50 of P. alata (ELPA) leaf extracts against both cancer (HCT-116, SF-295, OVACAR-8 and HL-60) cells and non-cancer cells (PBMC). (naturalnews.com)

Mutagenicity in mammalian cells: negative in CHO K-1 cells (OECD TG 476) (Hüls AG, 1998b). (europa.eu)

[ 1 ] However, they have a unique cell membrane that contains sterols, which are not present in either bacteria or viruses. (medscape.com)
Mycoplasma species have also been mistakenly believed to be L-forms of bacteria, which also lack cell walls. (medscape.com)
Unlike mycoplasmal organisms, L-form bacteria do not have sterols in the cell membranes, and they can revert to their walled parental forms. (medscape.com)

MSNs can easily internalize into Caco-2 cells without deleterious effects on cell viability. (springer.com)
The cell viability of Caco-2 cells was affected during MSNs-GA uptake. (springer.com)
In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)

On the cellular level, plasma effects include detachment of cells from the extracellular matrix and decreased migration velocity of cells. (nature.com)
During cell invasion, cells can modulate the environment as they migrate through the structure of the extracellular matrix (ECM). (sigmaaldrich.com)

The aim was to determine the effect of tannin on the nutritive value and microbial enzyme activity in vitro . (scielo.org.za)

Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
This can range from the relatively pedestrian, such as a person who received a bone marrow transplant, to creatures that seem more at home in science fiction, such as animals containing cells or tissues belonging to other species. (the-scientist.com)

Among the Passiflora leaf extracts evaluated, ELPA exhibited the highest cytotoxicity against HL-60 cells (IC50 19.37 u g/mL) but was not cytotoxic against PBMC, suggesting selectivity for cancer cells. (naturalnews.com)

1978. Altered erythrocyte membrane proteins in sickle cell patients associated with the severity of the disease. (mote.org)

Cellular assays, or cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. (sigmaaldrich.com)
Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and can assess compound characteristics simultaneously. (sigmaaldrich.com)

The generated series and parental molecule were tested in vitro for antiparasitic activity against Trypanosoma cruzi, Leishmania braziliensis, and L. amazonensis, and cytotoxicity using RAW cells. (lu.se)

Cryopreservation and transfer of gonads was shown to be an effective means in bird and mammalian species, while primordial germ cells offer possibilities and potential advantages in bird and fish species. (wur.nl)

However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
When AMPK expression was down-regulated using siRNA in vitro, pyrogallol reversed the reduced expression of AMPK. (oregonstate.edu)

While exerting marginal effects on non-neoplastic cells this unfolds promising applications in cancer therapy. (iiarjournals.org)

The passage number of cells used in the chromosomal aberration test was 40 for 24 hours treatment, 40 for 48 hours treatment by the direct method, and 23 by the metabolic activation method. (europa.eu)
There was no effect of EGCG on cell metabolic activity at any of the doses tested (0.3-30 µM). (molvis.org)

However, research on P. vivax remains severely hampered because, to date, attempts to maintain this parasite in routine in vitro blood cultures have been hindered by the strict restriction to invasion of reticulocytes, a minor short-lived fraction of peripheral blood. (nature.com)
However, such screening strategies are precluded for P. vivax because of the limited availability of infected blood from patients, an obstacle that would be circumvented should in vitro-cultured P. cynomolgi be available. (nature.com)
Caspofungin is extensively bound to albumin (~97%), and distribution into red blood cells is minimal. (theodora.com)
Samples of whole blood were collected and blood cells were challenged with acute doses of 0.1, 1 and 10 microM of (-)-nicotine for 60 minutes. (who.int)
1992. Flow cytometric DNA analysis of nurse shark (Ginglymostoma cirratum) and clearnose skate (Raja eglanteria) peripheral red blood cells. (mote.org)
Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology. (sigmaaldrich.com)
This can involve movement through the circulatory system and further through cells of the blood-brain-barrier (BBB). (sbir.gov)

The method was validated for three Meloidogyne species ( Meloidogyne javanica , M. incognita , and M. arenaria ), producing viable and robust freshly hatched J2s. (frontiersin.org)

The advantages of cell-based assays include the facilitation and generation of complex and biologically relevant data. (sigmaaldrich.com)
While Garry readily acknowledges the importance of multiple approaches, she said that there are important advantages to growing organs in developing animals rather than in vitro . (the-scientist.com)

2 of free radicals from the stimulation of available to the cell [ 1,2,7,8 ]. (who.int)

Cytogenicity in mammalian cells: negative in V79 CHL cells (OECD TG 473) (Degussa-Hüls AG, 1999). (europa.eu)

Now we are extending this approach of cross-species comparison to other mammalian species, including cows and rhinos, and other developmental processes. (irbbarcelona.org)

Prof. Tom Stout of the Faculty of Veterinary Medicine, Utrecht University, explained in detail the process of in vitro production (IVP) and cryopreservation and transfer of horse embryo's. (wur.nl)
Because of the large numbers, this work provides insights into factors affecting success rates of ovum pick-up and in vitro embryo production. (wur.nl)
Les effets in vitro du métronidazole sur la production d'espèces réactives de l'oxygène par les cellules polymorphonucléaires (PMN) ont été étudiés grâce au test au nitrobleu de tétrazolium et au test de chimioluminescence au luminol. (who.int)
Overall, our data suggest a prominent role for GAL3 in the aggregation process of αSYN and LB formation, leading to the production of short species to the detriment of larger strains which triggers neuronal degeneration in a mouse model of PD. (lu.se)
PMN results in the production of super- cer cells are known to be relatively resistant oxide radicals [ 7,8 ]. (who.int)

The Mexican yew is a rare species, only known to be found in a small number of locations in eastern Mexico, Guatemala, El Salvador and Honduras, and is listed as an endangered species. (wikipedia.org)

Neospora caninum is an apicomplexan parasite that infects a wide range of animal species. (usda.gov)

Organisms5

Diseases2

Vivo13

Reactive18

Assay15

Metabolism2

Antioxidant4

Pluripotent7

Apoptosis9

Mammalian cell1

Bovine5

Aqueous1

Proliferation9

Exposure5

Plasmodium3

20181

Especially for hypoxic cells2

Characterization3

Oocytes2

Study18

Mesenchymal Stem2

20191

Polymorphonuclear1

Inhibition2

Gene6

Alveolar macrophages2

Fungal cell wall2

Concentrations6

Toxicity2

Sperm2

Oxidative1

Human10

Culture8

Recombinant1

Genetic3

Stem cell3

Embryos3

Selectively1

1000 cells1

Plant species1

Epithelial cells2

Mononuclear cells1

Studies5

Researchers2

Mutagenicity1

Bacteria3

Viability3

Extracellular matrix2

Microbial1

Bone marrow3

Extracts1

Sickle cell1

Biochemical2

Cytotoxicity1

Germ1

Expression2

Effects1

Metabolic2

Blood7

Viable1

Advantages2

Free Radicals1

Cytogenicity1

Developmental1

Production5

Rare species1

Animal species1