• Description: A sandwich quantitative ELISA assay kit for detection of Human Annexin A1 (ANXA1) in samples from serum, plasma, tissue homogenates or other biological fluids. (jsce-ip.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Annexin A1 (ANXA1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (jsce-ip.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Annexin A1 (ANXA1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (jsce-ip.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Annexin A7 (ANXA7) in tissue homogenates, cell lysates and other biological fluids. (2sars.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Annexin A7 (ANXA7) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (2sars.com)
  • Add Annexin V conjugate assay solution. (aatbio.com)
  • Prepare an Annexin V-binding assay buffer: 10 mM HEPES, 140 mM NaCl, and 2.5 mM CaCl2, pH 7.4. (aatbio.com)
  • Resuspend cells in 200 μL of the Annexin V-binding assay buffer from Step 1. (aatbio.com)
  • Add 300 μL of the Annexin V-binding assay buffer (from Step 1) to increase volume before analyzing the cells with a flow cytometer or fluorescence microscope. (aatbio.com)
  • The molecular mechanisms of tea polyphenol-induced apoptosis as determined by annexin V apoptosis assay, DNA fragmentation, and caspase activation were further investigated. (tmu.edu.tw)
  • The following product was used in this experiment: Annexin A10 Monoclonal Antibody (1F9) from Thermo Fisher Scientific, catalog # MA5-21297, RRID AB_2576663. (thermofisher.cn)
  • IHC staining of FFPE human placenta with Annexin A1 antibody (clone ANXA1/3727). (nsjbio.com)
  • This Annexin A1 antibody is available for research use only. (nsjbio.com)
  • Optimal dilution of the Annexin A1 antibody should be determined by the researcher. (nsjbio.com)
  • Recombinant full-length human Annexin A1 protein was used as the immunogen for the Annexin A1 antibody. (nsjbio.com)
  • Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide). (nsjbio.com)
  • Differences in toxicity and reactivity based on valence state were determined in RLE-6TN rat alveolar epithelial and NR8383 rat alveolar macrophage cells using enhanced dark field microscopy, electron paramagnetic resonance (EPR), and annexin V/propidium iodide cell viability stain. (cdc.gov)
  • The protein family of annexins has continued to grow since their association with intracellular membranes was first reported in 1977. (wikipedia.org)
  • The criteria that a protein has to meet to be classified as an annexin are: it has to be capable of binding negatively charged phospholipids in a calcium dependent manner and must contain a 70 amino acid repeat sequence called an annexin repeat. (wikipedia.org)
  • In some annexins it can become phosphorylated and can cause affinity changes for calcium in the core region or alter cytoplasmic protein interaction. (wikipedia.org)
  • The four annexin repeats produce a curved protein and allow functional differences based on the structure of the curve. (wikipedia.org)
  • In all annexins the N-terminus is thought to sit inside the concave side of the annexin core and folds separately from the rest of the protein. (wikipedia.org)
  • Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells. (gzsys.org.cn)
  • Annexins have also been found outside the cell in the extracellular space and have been linked to fibrinolysis, coagulation, inflammation and apoptosis. (wikipedia.org)
  • Cell apoptosis was analyzed by Hoechst 33258 staining and annexin V-PI labeling. (molvis.org)
  • Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
  • Apoptosis of the cells was analyzed by flow cytometry using an Annexin V and propidium iodide binding method. (nel.edu)
  • Annexin V is a valuable tool for studying cell apoptosis. (aatbio.com)
  • Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). (aatbio.com)
  • Annexin A5 was proposed to use in detection of apoptosis. (cusabio.com)
  • The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. (nsjbio.com)
  • Studies have shown that xanthine dehydrogenase (XDH)-derived reactive oxygen species (ROS) accelerates tumor growth, and also induces mutations or produces cytotoxic effects concurrently. (medsci.org)
  • Reactive oxygen species (ROS) levels were determined using dihydrodichlorofluorescein staining, and the chymotrypsin-like protease activities were measured using the Suc-LLVY-aminoluciferin substrate. (molvis.org)
  • Mechanistically, both peptides caused a significant reduction at G0/G1 phase, in correlation with an augmented expression of the cell cycle inhibitory proteins p53, p27, p21, necrosis, exacerbated generation of reactive oxygen species and diminished mitochondrial membrane potential, all hallmarks of cellular stress. (nature.com)
  • Instead, we observed an increase in reactive oxygen species (ROS) production and a decrease of phosphorylated Akt as a result of DHEA and NALA treatment. (biomedcentral.com)
  • Loss of membrane potential and reactive oxygen species (ROS) generation were also detected by flow cytometry. (tmu.edu.tw)
  • High salt caused reduced spontaneous movement, degranulation and impaired production of reactive oxygen species (ROS) while leaving neutrophil viability unchanged. (frontiersin.org)
  • The effect of cerium oxide nanoparticle valence state on reactive oxygen species and toxicity. (cdc.gov)
  • Description: A sandwich ELISA kit for detection of Annexin A1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (jsce-ip.com)
  • Description: A sandwich ELISA kit for detection of Annexin A7 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (2sars.com)
  • Description: Quantitativesandwich ELISA kit for measuring Mouse Annexin A7 (ANXA7) in samples from serum, plasma, tissue homogenates. (2sars.com)
  • Annexin is a common name for a group of cellular proteins. (wikipedia.org)
  • As of 2002 160 annexin proteins have been identified in 65 different species. (wikipedia.org)
  • Several proteins consist of annexin with other domains like gelsolin. (wikipedia.org)
  • Annexins are a multigene family of Ca++-regulated phospholipid-binding proteins. (rndsystems.com)
  • The name annexin derives from the fact that initial studies suggested that acidic membrane phospholipids and Ca++ annexed, or recruited, special cytosolic proteins into a membrane-interacting ternary complex. (rndsystems.com)
  • Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. (aatbio.com)
  • ANXA4 can also interact with other annexin proteins or cytoskeletal proteins, potentially contributing to its diverse cellular functions. (creativebiomart.net)
  • Recombinant Human Annexin A13 (rHuAnnexin A13) is stored at -20°C. It is stable at 4°C for 1 week or -20°C for longer. (klamar-cn.com)
  • Recombinant annexin A2 prevents peripheral leukocyte account activation and also human brain infiltration soon after upsetting injury to the brain. (micrornainhibitors.com)
  • Flow cytometric analysis of HL-60 cells (blue) or K562 (green) using Annexin A2 (D11G2) Rabbit mAb (PE Conjugate) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) #5742 (dashed lines). (cellsignal.com)
  • Add 2 μL of the Annexin V conjugate to the cells. (aatbio.com)
  • The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. (wikipedia.org)
  • Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments. (rndsystems.com)
  • The fluorescent annexin V conjugates are stored in a PBS buffer solution containing 0.1% bovine serum albumin (BSA) with a pH of 7.4. (aatbio.com)
  • Serum level of annexin A5 is related to annexin A5 expression in cancer tissues. (cusabio.com)
  • Serum annexin A5 level was not altered in pre-eclampsia. (cusabio.com)
  • Serum annexin A2 levels in patients with colon cancer in comparison to healthy controls and in relation to tumor pathology. (gzsys.org.cn)
  • MIEN1, a novel interactor of Annexin A2, promotes tumor cell migration by enhancing AnxA2 cell surface expression. (gzsys.org.cn)
  • Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance. (nsjbio.com)
  • In patients with colon cancer, annexin A5 expression in cancer tissues is related to lymph node metastasis and tumor grade. (cusabio.com)
  • Relationship Between Circulating Tumor Cells and Annexin A2 in Early Breast Cancer Patients. (gzsys.org.cn)
  • Annexin A2 is a molecular target for TM601, a peptide with tumor-targeting and anti-angiogenic effects. (gzsys.org.cn)
  • A pair of annexin repeats may form one binding site for calcium and a phospholipid. (nsjbio.com)
  • Synthetic peptide surrounding aa 316 of human Annexin VIII. (covalab.com)
  • Reacts with human ANXA8 / Annexin A8. (covalab.com)
  • Annexin II promotes invasion and migration of human hepatocellular carcinoma cells in vitro via its interaction with HAb18G/CD147. (gzsys.org.cn)
  • Clinical significance of annexin II expression in human non-small cell lung cancer. (gzsys.org.cn)
  • Antigen standard for annexin A4 (ANXA4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
  • Annexins are important in various cellular and physiological processes such as providing a membrane scaffold, which is relevant to changes in the cell's shape. (wikipedia.org)
  • Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
  • It is not common to perform Annexin V binding flow cytometric analysis on adherent cells due to the possibility of membrane damage during cell detachment or harvesting. (aatbio.com)
  • Association of a characteristic membrane pattern of annexin A2 with high invasiveness and nodal status in colon adenocarcinoma. (gzsys.org.cn)
  • Also, annexins have been shown to be involved in trafficking and organization of vesicles, exocytosis, endocytosis and also calcium ion channel formation. (wikipedia.org)
  • The concave side of the annexin core interacts with the N-terminus and cytosolic second messengers, while the convex side of the annexin contains calcium binding sites. (wikipedia.org)
  • Short N-termini are thought to stabilize the annexin complex in order to increase calcium binding and can be the sites for post-translational modifications. (wikipedia.org)
  • Annexin V-FITC was used to detect apoptotic cells. (nel.edu)
  • Expression of annexin II in gastric carcinoma and its role in gastric cancer metastasis. (gzsys.org.cn)
  • Deregulated expression of annexin-A2 and galectin-3 is associated with metastasis in gastric cancer patients. (gzsys.org.cn)
  • Annexin A2 depletion delays EGFR endocytic trafficking via cofilin activation and enhances EGFR signaling and metastasis formation. (gzsys.org.cn)
  • Quantify Annexin V conjugates binding by using a flow cytometer with appropriate filters. (aatbio.com)
  • Note: Mouseover a species abbreviation on the product page to display the fullname. (novusbio.com)
  • increase in VEGF and Apelin, and decrease in Annexin A5 supports roles of hemo-dynamic alterations in fetoplacental circulation and structural alterations in uteroplacental bed occurring in preeclampsia. (cusabio.com)
  • In humans, the annexins are found inside the cell. (wikipedia.org)
  • Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration. (gzsys.org.cn)
  • S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility. (gzsys.org.cn)
  • The annexin core is highly conserved across the annexin family and the N-terminus varies greatly. (wikipedia.org)
  • This gene encodes a member of the annexin family. (thermofisher.cn)
  • Upregulated expression of annexin II is a prognostic marker for patients with gastric cancer. (gzsys.org.cn)
  • Several subfamilies of annexins have been identified based on structural and functional differences. (wikipedia.org)
  • Species-specific differences in the anti-proliferative effects of SGE were revealed, suggesting that the amount of active compounds in tick salivary glands change during feeding. (nel.edu)
  • Increased expression of the gene coding for annexin-1 is one of the mechanisms by which glucocorticoids (such as cortisol) inhibit inflammation. (wikipedia.org)
  • Long N-termini can contain up to 40 residues and have a more complex role in annexin signaling. (wikipedia.org)