• SpeB, the most abundant streptococcal extracellular protein, is a cysteine protease. (wikipedia.org)
  • It was originally studied as two separate toxins, streptococcal pyrogenic exotoxin B and streptococcal cysteine proteinase, until it was shown that both proteins were encoded by the speB gene and that the attributed pyrogenic activities were due to contamination by SpeA and SpeC. (wikipedia.org)
  • SpeB is known as streptococcal pyrogenic exotoxin B, streptopain and streptococcal cysteine proteinase as a result of its original misidentification as two separate toxins, and is neither an exotoxin nor pyrogenic. (wikipedia.org)
  • In Group A Streptococcus (GAS)-infected macrophages, Txnip was degraded independent of glucose consumption and streptococcal cysteine protease expression. (frontiersin.org)
  • The Majority of 9,729 Group A Streptococcus Strains Causing Disease Secrete SpeB Cysteine Protease: Pathogenesis Implications. (utswmed.org)
  • Dispersal of Group A streptococcal biofilms by the cysteine protease SpeB leads to increased disease severity in a murine model. (jefferson.edu)
  • However, despite its presence and high levels of conservation in the nucleotide sequence, 25-40% of these strains do not express the SpeB toxin in significant amounts. (wikipedia.org)
  • The speB and speJ genes are located in the core bacterial chromosome of all strains of S. pyogenes. (wikipedia.org)
  • SpeB is a 28 kDa protein with three major forms, mSpeB1, mSpeB2 and mSpeB3, which are categorized by variations the primary amino acid sequence. (wikipedia.org)
  • Although SpeB has crucial roles in modulating protein composition in EVs, the SpeB-positive EVs failed to trigger HaCaT keratinocytes pyroptosis, suggesting that EVs did not deliver SpeB into keratinocytes or the amount of SpeB in EVs was not sufficient to trigger cell pyroptosis. (bvsalud.org)
  • Finally, we identified that EV-associated enolase was resistant to SpeB degradation, and therefore could be utilized as the internal control protein for verifying SLO degradation. (bvsalud.org)
  • This study revealed that RopB would participate in modulating protein composition in EVs via SpeB-dependent protein degradation and suggested that enolase is a potential internal marker for studying GAS EVs. (bvsalud.org)
  • GSDMD , a substrate rather than an upstream protease, determines the occurrence of pyroptosis. (imrpress.com)
  • In this study, we found that the SpeB expression was higher in the ropB mutant than in the SIP-inactivated (SIP*) mutant. (bvsalud.org)
  • These findings reveal a crosstalk between the CovR/CovS and RopB-SIP systems and redefine the role of RopB in regulating speB expression in group A Streptococcus. (bvsalud.org)
  • PCR was performed to detect the expression of the protease-activated receptors (PARs) in MSCs. (biomedcentral.com)
  • The IgG proteases digest IgG from several species and subclasses to generate homogenous subunits that facilitate analytical or preparative workflows for the characterization, development and production of biopharmaceuticals. (genovis.com)
  • Our IgG and IgM proteases have specific enzymatic activities. (genovis.com)
  • Studies have shown that substrates, rather than their upstream proteases, determine the nature of cell death. (imrpress.com)
  • IgMBRAZOR is a unique IgM-specific protease that digests human IgM at one specific site. (genovis.com)
  • The streptococcal cysteine protease (SpeB) is one of the major virulence factors produced by group A streptococci (GAS). (nih.gov)
  • The mean amount of 28-kDa SpeB protein and cysteine protease activity produced by isolates from nonsevere cases was significantly higher than that from STSS cases (P = 0.001). (nih.gov)
  • SpeB, the most abundant streptococcal extracellular protein, is a cysteine protease. (wikipedia.org)
  • The Majority of 9,729 Group A Streptococcus Strains Causing Disease Secrete SpeB Cysteine Protease: Pathogenesis Implications. (utswmed.org)
  • It was originally studied as two separate toxins, streptococcal pyrogenic exotoxin B and streptococcal cysteine proteinase, until it was shown that both proteins were encoded by the speB gene and that the attributed pyrogenic activities were due to contamination by SpeA and SpeC. (wikipedia.org)
  • SpeB is known as streptococcal pyrogenic exotoxin B, streptopain and streptococcal cysteine proteinase as a result of its original misidentification as two separate toxins, and is neither an exotoxin nor pyrogenic. (wikipedia.org)
  • We examine whether this agonist, similar to the PAR1-activating protease thrombin or aPC respectively, induces the activation of the pro-inflammatory or anti-inflammatory signaling. (usz.ch)
  • Commensal as well as pathogenic bacteria secret various proteases that cleave PARs and act as inflammatory modulators. (usz.ch)
  • Our group is working on the protease-activated receptors (PARs), which play a major role in orchestrating the interactions between coagulation and inflammation. (usz.ch)