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CytokinesisTelophaseMitosisSpindle ApparatusMyosin Type IICell DivisionActomyosinAnaphaseContractile ProteinsSchizosaccharomycesSeptinsAurora Kinase BAurora KinasesMicrotubulesSchizosaccharomyces pombe ProteinsCell Cycle ProteinsMicrotubule-Associated ProteinsActinsKinesinCell Nucleus DivisionCentrosomeProtein-Serine-Threonine KinasesPolyploidyCell CycleMutationHeLa CellsChromosome SegregationMicroscopy, FluorescenceDictyosteliumProtein TransportCytoskeletal ProteinsCell PolarityGTPase-Activating ProteinsGreen Fluorescent ProteinsMicrofilament ProteinsCytoskeletonSpermatocytesRNA InterferenceRecombinant Fusion ProteinsHeterocyclic Compounds with 4 or More RingsSaccharomyces cerevisiae ProteinsProfilinsMyosinsTime-Lapse ImagingBrachiariarhoA GTP-Binding ProteinFungal ProteinsMolecular Sequence DataGuanine Nucleotide Exchange FactorsActin CytoskeletonModels, BiologicalAmino Acid Sequencerho GTP-Binding ProteinsMicroscopy, VideoCaenorhabditis elegansCell NucleusCaenorhabditis elegans ProteinsNocodazoleProtozoan ProteinsGTP PhosphohydrolasesSaccharomycetalesMeiosisTubulinSaccharomyces cerevisiaeMetaphaseDrosophila ProteinsEndosomal Sorting Complexes Required for TransportGTP Phosphohydrolase ActivatorsPhenotypeProtein BindingProtein Structure, TertiaryTrypanosoma brucei bruceiEmbryo, NonmammalianLuminescent ProteinsAurora Kinase CPlant CellsTwo-Hybrid System TechniquesQa-SNARE ProteinsTetraploidyChitin SynthaseCyclin BChromosomesSequence Homology, Amino AcidCDC2 Protein KinaseInterphasePhosphorylationrab GTP-Binding ProteinsMyosin Heavy ChainsGametogenesis, PlantGenes, FungalPolar BodiesCell MembraneFluorescence Recovery After PhotobleachingDrosophila melanogasterRho Guanine Nucleotide Exchange FactorsRNA, Small InterferingDrosophilaProtein KinasesCell LineEndosomes
RegulationMitosisCleavageCdc42ActinFissionPoorly understoodMitoticConstrictionInhibitionSpindleProliferationUtilizeReplicationControlAbsenceMolecularCellsCell divisionDivisionDistributionContractileLateCompleteCompletion of cytokinesisStages of cytokinesisSuccessful cytokinesisTemporal and spatialOrganizationMicrotubuleVitroEndocytosisCuesProteinMechanismsMammalianTranscriptionalAlignmentProteinsProcessesDistinctContractionStructuralTissueFormationDataMethodsGrowthCell wallDevelopmentDirectionEvents
Regulation6
  • Furthermore, our data show that the MinCDE system indeed is sufficient to cause spatial regulation of FtsZ, required for Z-ring positioning. (elsevierpure.com)
  • Arumugam, S , Petrasek, Z & Schwille, P 2014, ' MinCDE exploits the dynamic nature of FtsZ filaments for its spatial regulation ', Proceedings of the National Academy of Sciences of the United States of America , vol. 111, pp. 1192-1200. (elsevierpure.com)
  • Scaffold proteins are known to physically tether client proteins to specific cellular areas, functioning in spatial regulation of biological processes including signaling transduction, cytokinesis, morphogenesis, and ACD. (nature.com)
  • Our results reveal a conserved signaling network, termed the Septation Initiation Network (SIN), of which Sid1p is an intermediate component, that controls temporal and spatial regulation of cytokinesis. (umassmed.edu)
  • In this proposal, a combination of biochemical approaches and live-cell studies will be employed to gain insights into the spatial and temporal regulation of Kif18A in mammalian cells. (uni-konstanz.de)
  • We hypothesize that temporal and spatial regulation of abscission helps expand the early stem cell pool, and influences daughter cell fates. (virginia.edu)
Mitosis5
  • Cytokinesis must only occur after chromosomes have segregated during mitosis to ensure each daughter cell receives the proper complement of genetic material. (umassmed.edu)
  • We conclude that signaling cytokinesis is tightly coordinated with mitosis in S. pombe by positive signals acting through Sid1p and the SIN, and under certain conditions, negative signals acting through Dma1p. (umassmed.edu)
  • In the present study, we examined cytokinesis in ammonia-activated sea urchin eggs, which lack paternally derived centrosomes and undergo mitosis mediated by unusual anastral, bipolar mini-spindles. (mblwhoilibrary.org)
  • In parallel, a small molecule screen will we performed to identify inhibitors of Kif18A that allow us to dissect potential functions of Kif18A in late mitosis and/or cytokinesis, without interfering with its reported function in prometaphase. (uni-konstanz.de)
  • This later on pattern is definitely more obvious in mitosis and cytokinesis. (healthandwellnesssource.org)
Cleavage3
  • The ability of microtubules of the mitotic apparatus to control the positioning and initiation of the cleavage furrow during cytokinesis was first established from studies on early echinoderm embryos. (mblwhoilibrary.org)
  • Treatments with a DNA synthesis inhibitor, aphidicolin, show that the number of nuclear divisions, and perhaps the DNA to cytoplasmic ratio, are critical for the appearance of lineage-specific differentiation.Conclusion: Our work corroborates previous studies demonstrating that the cleavage program is causally involved in the spatial segregation and/or activation of factors that give rise to distinct cell types in ctenophore development. (illinois.edu)
  • When the cells split in two during cytokinesis, the cleavage furrow completes with a final bridge between the two cells called the midbody. (virginia.edu)
Cdc421
  • Although DOCK8 plays a critical role in spatial control of Cdc42 activity during interstitial leukocyte migration, the mechanism remains unclear. (life-science-alliance.org)
Actin5
  • We show that the actin nucleation factor SCAR/WAVE is strongly activated during Dictyostelium cytokinesis. (biologists.com)
  • Spatial and temporary control of actin redesigning can be needed for this multi-stage transportation procedure that can be important for the creation of a practical egg. (careersfromscience.org)
  • Cytokinesis occurs mechanically through constriction of an actin-myosin based contractile ring, while initiation of ring constriction is temporally and spatially mediated by complex signaling networks. (umassmed.edu)
  • Eukaryotic cells rely on de novo nucleation mechanisms to generate actin filaments in order to elicit spatial and temporal remodeling of their actin cytoskeleton. (biomedcentral.com)
  • Actin is usually involved in cytokinesis, muscle contraction, and cell movement. (aatbio.com)
Fission1
Poorly understood2
  • While the sub-types of hemocytes that circulate within the mosquito hemocoel are known, basic aspects of hemocyte biology, such as hemocyte numbers, proliferation and spatial distribution, remain poorly understood. (biomedcentral.com)
  • We hypothesized that to achieve such tight control of cytokinesis, cells may utilize both promotional and inhibitory signals, however how cells maintained this control was poorly understood. (umassmed.edu)
Mitotic2
  • We found Sid1p is additionally controlled by Cyclin Dependent Kinase activity, uncovering an important link between mitotic events and initiation of cytokinesis. (umassmed.edu)
  • Our results highlight the self-organizing nature of the central spindle region and its ability to induce cytokinesis-like furrowing, even in the absence of a complete mitotic apparatus. (mblwhoilibrary.org)
Constriction1
  • 1) We first sought to characterize the positive timing mechanism that signals cytokinesis though a detailed investigation of Sid1p, a protein kinase essential for activation of ring constriction. (umassmed.edu)
Inhibition2
  • 2) Secondly, we sought to define how cells negatively regulate cytokinesis through investigation of Dma1p, a spindle checkpoint protein implicated in inhibition of cytokinesis. (umassmed.edu)
  • The cytoskeleton also influences binding or inhibition to phospholipids, membrane trafficking, ion transport, and spatial localization of signaling. (aatbio.com)
Spindle1
Proliferation1
  • The DNA-helicase HELLS drives ALK ALCL proliferation by the transcriptional control of a cytokinesis-related program. (cornell.edu)
Utilize1
Replication2
  • The replication of the kDNA is definitely a complex process that takes place in a highly structured spatial and temporal pattern. (healthandwellnesssource.org)
  • In schizogony a single parasite first generates many nuclei via independent, asynchronous rounds of genome replication, prior to cytokinesis which is the physical division of the cell. (europa.eu)
Control2
  • Considering the conservation of cell cycle regulators in the eukaryotic kingdom, it is likely that similar mechanisms to control cytokinesis exist in humans. (umassmed.edu)
  • The control objective is to maintain a desired spatial temperature distribution in the incoming material, which is fed into the furnace by rollers, the speed of which is regulated by a speed controller. (scholarpedia.org)
Absence1
  • Interestingly, even in the absence of cytokinesis, differentiation markers appear to be activated at the correct times. (illinois.edu)
Molecular1
Cells2
  • Transportation happens through the band waterways, remains of imperfect cytokinesis, that connect the doctor cells to each additional and the oocyte. (careersfromscience.org)
  • Cytokinesis is the final event of the cell division cycle and results in physical and irreversible separation of a mother cell into two daughter cells. (umassmed.edu)
Cell division1
Division1
  • We demonstrate that SCAR activity is essential to drive myosin-II-independent cytokinesis, and stabilises the furrow, ensuring symmetrical division. (biologists.com)
Distribution1
  • These data describe the spatial and temporal distribution of mosquito hemocytes, and map the cellular response to infection throughout the hemocoel. (biomedcentral.com)
Contractile1
Late1
Complete1
  • We present the case of a young patient with a large spinal tumor who, after neoadjuvant Denosumab, underwent complete en bloc surgery with clean margins and a great pathological response. (bvsalud.org)
Completion of cytokinesis3
  • Chen X.-W., Inoue M., Hsu S.C., Saltiel A.R., RalA-exocyst-dependent recycling endosome trafficking is required for the completion of cytokinesis. (biologie-journal.org)
  • Emoto K., Inadome H., Kanaho Y., Narumiya S., Umeda M., Local change in phospholipid composition at the cleavage furrow is essential for completion of cytokinesis. (biologie-journal.org)
  • Required for completion of cytokinesis (PubMed:17853893, PubMed:17556548, PubMed:18641129). (nih.gov)
Stages of cytokinesis2
  • Elad N., Abramovitch S., Sabanay H., Medalia O., Microtubule organization in the final stages of cytokinesis as revealed by cryo-electron tomography. (biologie-journal.org)
  • The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17853893, PubMed:17556548). (nih.gov)
Successful cytokinesis1
Temporal and spatial4
  • Introduction Temporal and spatial coordination of the process of mitosis and cytokinesis is a prerequisite for accurate and equal segregation of genomic and cytosolic material into two daughter cells. (forumbcn2004.org)
  • Yeast possesses both forward and feedback system to achieve temporal and spatial regulation of cell wall construction, Forward system is composed of a cell cycle dependent morphological pathway that is regulated by small GTPase, Rho1p. (u-tokyo.ac.jp)
  • Much of the existing cytoskeleton remains intact during these processes, necessitating a very precise temporal and spatial duplication and segregation of the many single-copy organelles. (ox.ac.uk)
  • Alternatives to gene deletions are mutants that are conditional, for e.g, temperature sensitive (ts) mutants are robust tools to understand temporal and spatial functions of genes. (iisc.ac.in)
Organization4
  • Spatial organization of actin filaments, whose growth generates membrane deformations during motility 1, phagocytosis 2, endocytosis 3, and cytokinesis 4, is mediated by specific protein-protein interactions that branch, crosslink, and bundle filaments into networks that interact with the membrane. (researchgate.net)
  • These molecules associate with cell membranes, actin, cytoskeleton microtubules and they are related to a number of processes that take place in the cell cortex and that require spatial organization, such as cytokinesis, cell cycle, diffusion barrier formation and spindle alignment. (usp.br)
  • The Chang Lab studies fundamental questions concerning spatial organization within a single cell: How are cell shape and size determined? (ucsf.edu)
  • We pioneered the use of single-molecule localization microscopy (SMLM) based superresolution imaging for microbiology and illustrated the spatial organization of the FtsZ-ring in live E. coli cells for the first time. (jhmi.edu)
Microtubule4
  • Here, we show that Leishmania coronin preferentially distributes to the distal tip during cytokinesis, and interacts with microtubules through a microtubule-based motor, kinesin K39. (silverchair.com)
  • These results indicate that coronin regulates microtubule remodeling during Leishmania cytokinesis and is essentially required for survival of these parasites in culture. (silverchair.com)
  • Byers B., Abramson D.H., Cytokinesis in HeLa: post-telophase delay and microtubule-associated motility. (biologie-journal.org)
  • Current projects include: regulation of cell size and growth, cytoplasmic density and turgor pressure, cell wall assembly, cytokinesis, and microtubule regulation. (ucsf.edu)
Vitro1
  • Assessment of the aneugenic potential of selected oestrogens, progestins and androgens using the in vitro cytokinesis blocked micronucleus assay. (hewedi.com)
Endocytosis1
  • Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. (nih.gov)
Cues1
  • The activity of Cdc42 is dynamically controlled in time and space to enable distinct polarization events, which generally occur along a single axis in response to spatial cues. (microbialcell.com)
Protein2
Mechanisms2
  • The mechanisms of membrane growth during cytokinesis are largely unknown. (rupress.org)
  • Our goal is to summarize the mechanisms of structural variations in tumorigenesis from both molecular mechanisms and spatial structure, to convey a novel perspective for clinical therapies and resistance prevention. (biomedcentral.com)
Mammalian1
  • Silencing of HSPB8, like depletion of BAG3, inhibited targeting of ubiquitinated proteins to the juxtanuclear aggresome, a mammalian system of spatial quality control. (nih.gov)
Transcriptional2
  • We introduce Single-cell Nanopore Spatial Transcriptomics ( scNaST ), a software suite to facilitate the analysis of spatial gene expression from second- and third-generation sequencing, allowing to generate a full-length near-single-cell transcriptional landscape of the tissue microenvironment. (frontiersin.org)
  • The DNA-helicase HELLS drives ALK ALCL proliferation by the transcriptional control of a cytokinesis-related program. (cornell.edu)
Alignment2
  • This finding has wide-ranging implications for the understanding of eukaryotic cytokinesis, because all previous studies that describe vesicle alignment and fusion have relied upon chemical fixation. (biologists.com)
  • In gauging the suitability of a particular light source, the important parameters are structure (the spatial distribution of light, source geometry, coherence, and alignment), the wavelength distribution, spatial and temporal stability, brightness, and to what degree these various parameters can be controlled. (fsu.edu)
Proteins2
  • The rest of the 38 healthy proteins had been discovered within the cytoplasm and also revealed various spatial patterns. (mirnaarray.com)
  • Biphasic Cdc42 polarization is likely to ensure the proper timing of events including the assembly and recognition of spatial landmarks and stepwise assembly of a new ring of septins, cytoskeletal GTP-binding proteins, at the incipient bud site. (microbialcell.com)
Processes1
  • Molecular dynamics simulations offer a complementary approach, providing extremely high resolution spatial and temporal data on folding processes. (researchgate.net)
Distinct2
  • Cascone I., Selimoglu R., Ozdemir C., Del Nery E., Yeaman C., White M., Camonis J., Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs. (biologie-journal.org)
  • Estey M.P., Di Ciano-Oliveira C., Froese C.D., Bejide M.T., Trimble W.S., Distinct roles of septins in cytokinesis: SEPT9 mediates midbody abscission. (biologie-journal.org)
Contraction1
  • This is followed by cytokinesis (approximately 15-20 minutes), when the cell contraction in the middle and physically separates into two daughter cells. (cellimagelibrary.org)
Structural1
  • Precision of structural mechanics of cytokinesis is however probably no better illustrated than in the protozoa. (ox.ac.uk)
Tissue3
  • MI is a complex spatio-temporal heterogeneous disease involving the whole heart, and unbiased spatial transcriptomics holds a promise to add tissue context to molecular profiling in the search of novel therapeutics. (frontiersin.org)
  • After the tissue section is fixed on the spatial slide, stained, and imaged, it is permeabilized to release RNA to bind to capture probes for on-slide cDNA synthesis. (frontiersin.org)
  • Library preparation is performed off-slide, and spatial barcodes and tissue image are used to overlay transcriptomics data with tissue context. (frontiersin.org)
Formation1
Data1
  • Taking advantage of the Visium Spatial platform, we adapted a strategy recently developed to assign barcodes to long-read single-cell sequencing data for spatial capture technology. (frontiersin.org)
Methods1
  • One of the most established spatial transcriptomics methods is now widely available as the Visium platform by 10x Genomics. (frontiersin.org)
Growth1
  • Cytokinesis is essential for the growth and development of new cells. (nih.gov)
Cell wall1
Development2
  • Recent development in spatial transcriptomics addresses this challenge, but few studies only have provided spatially resolved insights into the cardiac transcriptome. (frontiersin.org)
  • Development of an active viscous thin shell theory to simulate cytokinesis and surface buckling of the cell cortex. (navier-lab.fr)
Direction1
  • Because this enhanced damping arises from the entanglement of two networks incurring a strain-induced jamming transition at subcritical volume fractions, which is demonstrated through the spatial correlation of velocity direction, we term this phenomenon "topological damping. (bvsalud.org)
Events1