• Reporter assays identified a region of 500bp around the transcription start site that was optimally active in brain endothelium. (open.ac.uk)
  • In transient transfection assays, 2.5 kb of the ACLP 5′-flanking sequence directed high levels of luciferase reporter activity in primary cultured rat aortic smooth muscle cells, and this activity was not dependent on serum response factor. (ahajournals.org)
  • They bind LCE promoters and overlap SP1 and SP3 sites, and there is a reciprocal relationship between TALE and SP binding on external (e.g. skin) and internal (e.g. tongue) LCE promoters. (biologists.org)
  • The atherogenic cytokine IL-6 (interleukin-6) induces pro-inflammatory gene expression in VECs (vascular endothelial cells) by activating the JAK (Janus kinase)/STAT3 (signal transducer and activator of transcription 3) signalling pathway, which is normally down-regulated by the STAT3-dependent induction of the E3 ubiquitin ligase component SOCS3 (suppressor of cytokine signalling 3). (biochemj.org)
  • The PDPN promoter is active, when, accompanied by distal hypermethylation, several factors accumulate near Sp1/Sp3 proteins bound at sites bp -700/-696 (Sp1.4) and bp -100/-95 (Sp1.2). (nih.gov)
  • The aim of this study was to identify cell-specific controls on the transcription of MDR1 in human brain endothelium. (open.ac.uk)
  • During inflammation, proinflammatory stimuli, including LPS, TNFα or IL-1β, cause phenotypic changes to the quiescent endothelium by inducing the expression of proinflammatory factors such as IL-6 and IL-8 or adhesion molecules such as ICAM-1 and VCAM-1. (biomedcentral.com)
  • A factor located downstream from bp -38 and associated with Sp1/Sp3 bound to site Sp.1 may exert a repressive effect. (nih.gov)
  • Characterization of human epidermal growth factor receptor and c-src interactions in human breast tumor cells. (springer.com)
  • Since recent findings have focussed on podoplanin's potential role as a tumour progression factor, we aimed at identifying regulatory elements conferring PDPN promoter activity. (nih.gov)
  • Chronic inflammation is associated with increased inflammatory activity associated with increased levels of pro-inflammatory cytokines in the circulation, including IL-1 (IL is interleukin), IL-6 and TNFα (tumour necrosis factor α) [ 1 , 2 ]. (biochemj.org)
  • Cutting-edge studies reported that high-risk transformation ability of adipose tissue is due to production of different pro-inflammatory cytokines like IL-8, IL-6 or IL-2 and other enzymes like lactate dehydrogenase (LDH) and tumour necrosis factor alpha (TNFα). (biomedcentral.com)
  • Deletion studies demonstrated the functional importance of two Sp1/Sp3 sites for PDPN promoter activity. (nih.gov)
  • p75NTR is almost invariably expressed after tissue injury that is accompanied by edema, and Peterson and Bogenmann (2003) have proposed that injury-induced reductions in extracellular osmolarity activate p75NTR transcription in vivo . (jneurosci.org)
  • SP3 is involved in various intracellular processes, including cell cycle regulation, hormone induction and housekeeping activities. (thefreedictionary.com)
  • Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that are implicated in the regulation of lipid and glucose homeostasis. (biomedcentral.com)
  • We demonstrate here that a complex of TALE homeobox transcription factors PBX1, PBX2 and Pknox (homologues of Drosophila Extradenticle and Homothorax) preferentially regulate external rather than internal LCE gene expression, competitively binding with SP1 and SP3. (biologists.org)
  • Transcription of the caveolin-1 gene is differentially regulated in lung type I epithelial and endothelial cell lines. (bu.edu)
  • Tyrosine kinase signalling in breast cancer: Epidermal growth factor receptor and c-src interactions in breast cancer. (springer.com)
  • In summary, Atox1 functions not only as a copper chaperone for SOD3 but also as a positive regulator for SOD3 transcription and may have an important role in modulating oxidative stress in the cardiovascular system. (ahajournals.org)
  • Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. (genecards.org)
  • Indeed, the ability of flavanoids to induce SOCS3 expression requires activation of the ERK (extracellular-signal-regulated kinase)-dependent transcription factor SP3, and not STAT3. (biochemj.org)
  • Concomitantly, treatment with the DNA methyltransferase inhibitor 5-azaCdR in combination with trichostatin A (TSA) downregulated podoplanin mRNA levels in MG63 cells, and region-specific in vitro methylation of the distal promoter suggested that DNA methylation rather enhanced than hindered PDPN transcription in both cell types. (nih.gov)