Loading...
  • putative
  • Studies employing plasmids containing parts of the 165 bp of p57 Kip2 promoter indicate that the promoter region between −87 and −113 bp, which includes two putative Sp1 consensus sequences, plays a critical role in the response to HDACIs. (oup.com)
  • We will further present binding characteristics of the putative transcription factor to the cis-acting element in breast cancer cells. (aacrjournals.org)
  • Promoter region contains, by sequence similarity, 2 forward and two reverse Sp1-like binding sites, four putative glucocorticoid/progesterone response elements (PREs), cAMP responsive element (CRE) and activator protein-1 (AP-1) element. (atlasgeneticsoncology.org)
  • We cloned and sequenced 2056 nucleotides from the 5'-flanking region of the PDPN gene and a computational search revealed that the TATA and CAAT box-lacking promoter possesses features of a growth-related gene, such as a GC-rich 5' region and the presence of multiple putative Sp1, AP-4 and NF-1 sites. (nih.gov)
  • specificity protein
  • The purpose of this study was to investigate the anti-proliferative effects of 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) on two oral squamous cell carcinoma (OSCC) cell lines, HN22 and HSC4, through regulation of specificity protein 1 (Sp1). (nih.gov)
  • motifs
  • The broad applicability of this site-specific ChIP could be demonstrated by analyzing these SP1 motifs in both osteosarcoma cells and kidney carcinoma tissue. (biomedsearch.com)
  • apoptotic
  • The Maillard reaction is a chemical reaction occurring between a reducing sugar and an amino acid, generally requiring thermal processing.HPB242 treatment dramatically reduced the cell growth rate and apoptotic cell morphologies.Sp1 was significantly inhibited by HPB242 in a dose-dependent manner. (nih.gov)
  • Thus, a target of oxidative stress in HCV infection is DHCR24 through Sp1, which suppresses apoptotic responses and increases tumorigenicity. (mdpi.com)
  • recombinant
  • Recombinant human (rhu) Epo, Steel factor (SLF), and granulocyte colony-stimulating factor (G-CSF) were purchased from Amgen Corp and R & D Systems (Thousand Oaks, CA). Rhu granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3) were kind gifts from Immunex (Seattle, WA). (bloodjournal.org)
  • mRNA
  • Finally, electroporation of Sp1 or Sp4 cDNA into cultures of DRG neurons directed an increase in Sp1/Sp4 mRNA and importantly an increase in TRPV1 mRNA. (biomedcentral.com)
  • While miR-126 bound directly to the 3′-untranslated region of CCL2 mRNA, miR-193b regulated CCL2 production indirectly through a network of transcription factors, many of which have been identified in other inflammatory conditions. (diabetesjournals.org)
  • MicroRNAs (miRNAs) have emerged as important factors regulating gene expression through binding to complementary sequences of target mRNAs resulting in decreased mRNA levels ( 10 ). (diabetesjournals.org)
  • epithelial
  • The authors reported previously that FN induces α5 expression in human corneal epithelial cells and rabbit corneal epithelial cells by altering the binding of the transcription factor (TF) Sp1 to a regulatory element from the α5 promoter that it is also flanked by binding sites for the TFs NFI and AP-1. (ulaval.ca)
  • These results suggest that α5 gene expression is likely dictated by subtle alterations in the nuclear ratio of TFs that either repress (NFI) or activate (Sp1 and AP-1) α5 transcription in corneal epithelial cells. (ulaval.ca)
  • repressor
  • Whereas both AP-1 and Sp1 activated expression directed by the α5 promoter, NFI functioned as a potent repressor of that gene. (ulaval.ca)
  • In SL2 cells, Sp3 functioned as a repressor, while Sp1 and Sp3 acted positively synergistic. (nih.gov)
  • Molecules
  • Recent studies have (1) revealed new mechanisms by which steroid receptors modulate transcription and activate cell signaling pathways, (2) identified molecules with diverse functions that regulate steroid receptor action, and (3) elucidated roles for steroid receptors in both normal physiological processes and disease states, including cancer. (keystonesymposia.org)
  • vitro
  • A truncation mutant of LEF-1 (HMG-88), which contains the HMG box but lacks the trans-activation domain, did not activate transcription from nucleosomal DNA, indicating that bending of DNA by the HMG domain is not sufficient to activate transcription in vitro. (ac.be)
  • Granulocyte-Macrop
  • An unusual cell cycle profile with a majority of cells in S phase (70.2%) and minority of cells in G1 phase (27.4%) was observed in burst-forming unit-erythrocytes (BFU-E)-derived erythroblasts from a 7-day culture of CD34 + cells stimulated with interleukin 3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), Steel factor, and Epo. (bloodjournal.org)
  • downstream
  • Growth factors, cytokines and their receptors as downstream targets of arylhydrocarbon receptor (AhR) signaling pathways. (wikipathways.org)
  • A factor located downstream from bp -38 and associated with Sp1/Sp3 bound to site Sp.1 may exert a repressive effect. (nih.gov)
  • absence
  • ChIP also revealed that histone H4 at the same locus is hyperacetylated in YMB-1 cells, indicating that the promoter is transcriptionally active in the absence of Sp1 and HNF-4 bindings. (aacrjournals.org)
  • tissue
  • Binding of fVII to tissue factor (TF) at sites of vascular injury triggers coagulation and hemostasis. (aacrjournals.org)
  • Using ChIP analysis of DRG tissue, we demonstrated that Sp1 and Sp4 are bound to the candidate GC-box site region within the endogenous TRPV1 P2-promoter. (biomedcentral.com)
  • cell
  • Our results indicate that Sp1 coordinately regulates the expression of FAS and cyclin D1 in MCF-7 cells, suggesting that Sp1 is involved in synchronizing fatty acid synthesis with cell cycle progression. (aacrjournals.org)
  • As expected, we found that both Sp1 and HNF-4 bind to the promoter region in a hepatic cancer cell HepG2. (aacrjournals.org)
  • Coexpression of Sp1, NFI, and AP-1 was demonstrated in all cell types, and each TF was shown to bind efficiently to the α5 promoter. (ulaval.ca)
  • An unresolved aspect of current understanding of erythroid cell-specific gene expression relates to how a limited number of transcriptional factors cooperate to direct high-level expression mediated by cis-regulatory elements separated over large distances within globin loci. (asm.org)
  • Several transcription factors have been shown to be involved in the pathogenesis of neuroblastoma by enhancing cancer cell proliferation ( 7 ). (spandidos-publications.com)
  • Cell models of endocrine resistance have demonstrated a role for altered growth factor signalling in the development of an endocrine insensitive phenotype. (springer.com)
  • binding site
  • In particular, we have studied the SP1 binding site within the promoter region of the c-KIT oncogene and found that this site can fold into an anti-parallel two-tetrad G-quadruplex. (semanticscholar.org)
  • Nerve Growth
  • Vitamin D has also been implicated in PD through its effects on L-type voltage-sensitive calcium channels (L-VSCC), nerve growth factor (NGF), matrix metalloproteinases (MMPs), prostaglandins (PGs) and cyclooxygenase-2 (COX-2), reactive oxygen species (ROS), and nitric oxide synthase (NOS). (hindawi.com)
  • Despite
  • Despite the dense arrangement of the Sp1 TFBSs the improved ChIP method was able to individually examine the allocation of all adjacent Sp1 TFBS at once. (biomedsearch.com)
  • cancer
  • The synchronization of these two biological processes by Sp1 could be exploited as a strategy for cancer therapy. (aacrjournals.org)
  • Tyrosine kinase signalling in breast cancer: Epidermal growth factor receptor and c-src interactions in breast cancer. (springer.com)