Sp1 in vivo sp1 transcription factor. Medical search. Frequent questions

Moreover, by using a GAL4-Sp1 fusion protein, we have directly demonstrated that Rb positively regulates Sp1 transcriptional activity in vivo. (umn.edu)
Molecular studies in Ino80 KO ESCs demonstrated that INO80 is bound to the Bmp4 promoter, and regulates its chromatin structure, to suppress the positive regulator SP1 from stimulating its transcription. (vcu.edu)
The mesenchymal phenotype is associated with high expression levels of Snai1, a zinc-finger transcription factor that regulates metastasis by down-regulating the expression of genes such as E-cadherin ( 17 ). (iiarjournals.org)
We previously showed that peroxisome proliferator-activated receptor (PPAR)-γ in β-cells regulates pdx-1 transcription through a functional PPAR response element (PPRE). (diabetesjournals.org)
In vitro studies of INS-1 cells confirmed that PPAR-γ binds to the putative PPRE sequence and regulates GIP-R transcription. (diabetesjournals.org)

Moreover, biological experiments showed that LINC01376 facilitated the proliferative, invasive, and migratory abilities of NPC cells in vitro and in vivo. (iubmb.org)
Analysis of the murine 5-HT receptor gene promoter in vitro and in vivo. (ox.ac.uk)
We have recently demonstrated through in vitro and in vivo studies that the more effective methylated form, PRIMA-1 Met , can display a potent anti-myeloma activity without requiring functional activation of p53, which is associated with activation of p63/73 signaling pathway and down-regulation of c-Myc [ 9 ]. (oncotarget.com)
Ino80 KO ESCs (Embryonic stem cells) were viable when maintained at ground state pluripotency but fail to differentiate in vitro and in vivo . (vcu.edu)

Sp1, an ATM-regulated transcription factor, is found to increase BORC-subunit genes expression after radiation. (tmu.edu.tw)
This plethora of Hox protein-protein interactions clearly points to a Hox-driven transcription process in which every Hox homeoprotein could selectively recruit GTFs to achieve specificity and activate or repress target genes during Drosophila development, although the precise molecular mechanisms remain elusive [ 25 ]. (biomedcentral.com)
Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. (novusbio.com)
It selectively reduces transcription of genes that have promoters controlled by the Sp1 factor, such as survivin, cdc2 and VEGF. (erimos.com)
The specificity of terameprocol in cancer cells is derived from being able to access those genes that are highly active in cancer (through their "open" DNA conformation) to repress their expression through Sp1 binding inhibition, while normal cells do not have the same gene expression profile or DNA conformation accessible to terameprocol binding. (erimos.com)
Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)

With a series of deletion/point mutants, we identified CaM antagonist-responsive region in the putative Sp1-binding domain between -295 to -300 base pairs of DR5 gene. (oncotarget.com)

Peak evaluation is complex, as gene expression regulation involves interactions between combinatorial transcription factor binding sites and chromatin states. (biomedcentral.com)
Deletion of the ATP-dependent chromatin remodeling factor INO80 is known to be embryonically lethal, however, the mechanism is not known. (vcu.edu)
NURF (Nucleosome remodeling factor) is another ATP-dependent chromatin remodeling complex that is overexpressed in many cancer types including breast cancer. (vcu.edu)
Chromatin immunoprecipitation assay, siRNA, and luciferase gene transcription assay in INS-1 cells were performed. (diabetesjournals.org)

SP1 knockdown by siRNA abolished the DFP-induced increase in the mRNA levels of FTMT, indicating SP1-mediated regulation of FTMT expression in the presence of DFP. (wikipedia.org)
Furthermore, the knockdown of Sp1 inhibited the expression of collagen type I (CoL-I) and fibronectin (Fn). (ijbs.com)

miR-375-5p microRNA significantly decreased expression of SP1 and YAP1 in colorectal cancer cells. (wikipedia.org)
Alcohol consumption is a risk factor for many types of cancer, including breast and colorectal cancer ( 4 ). (iiarjournals.org)

Mechanistically, Sp1 promoted the expression levels of CoL-I and Fn by directly binding to the promoter of Gm26669 to elevate its expression level. (ijbs.com)

Furthermore, LINC01376 competitively sponged the transcription factor SP1 and reduced its enrichment in the upstream promoter region of miR-4757 to repress miR-4757 expression. (iubmb.org)
We used ligation-mediated polymerase chain reaction (PCR) for a genomic sequencing study in which 450 bp of the human PGK-1 promoter region was analyzed for the presence of in vivo protein footprints and cytosine methylation at all CpG sites. (oregonstate.edu)
Survivin especially is almost exclusively present in tumor cells and terameprocol can inhibit its production by accessing the promoter region of the survivin gene to prevent Sp1 binding. (erimos.com)

These data were recapitulated in vivo, where G/G homozygotes had significantly higher resistin mRNA levels in human abdominal subcutaneous fat. (diabetesjournals.org)

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. (wikipedia.org)

In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1. (oregonstate.edu)
Primary sequence and epigenetic determinants of in vivo occupancy of genomic DNA by GATA1. (jamestaylor.org)

Treatment with Deferiprone increased the expression of cytoplasmic and nuclear SP1 with predominant localization in the nucleus. (wikipedia.org)
In the present study, we observed a significant increase in the nuclear translocation of Sp1 using both in vivo and in vitro models of RIF. (ijbs.com)
Moreover, we found that NCTD alleviated RIF by inhibiting Gm26669 and the nuclear translocation of Sp1. (ijbs.com)
After hyperoxia, HO-1(CM) -/- hearts showed suppression of the Pgc-1α/nuclear respiratory factor-1 (NRF-1) axis, swelling, low electron density mitochondria by electron microscopy (EM), increased cell death, and extensive collagen deposition. (jci.org)
In addition, nuclear factor κB (NF‑κB), c‑Jun N‑terminal kinase (JNK), extracellular signal‑regulated kinases and p38 mitogen‑activated protein kinase inhibitors and TNF‑α were used to determine the molecular mechanism of autophagy during the inflammatory conditions, and only the NF‑κB and JNK inhibitor were found to enhance the autophagy of rat NP cells. (spandidos-publications.com)

Two copies of a motif (the retinoblastoma control element) similar to that found in the human c-fos, transforming growth factor β1, and c-myc promoters are responsible for conferring Rb regulation to the fourth promoter of the insulinlike growth factor II gene. (umn.edu)
This is achieved through the deactivation of viral Sp1-dependent promoters that are essential for viral propagation. (erimos.com)

SP1 most notably contains three zinc finger protein motifs at its C-terminus, by which it binds directly to DNA and allows for interaction of the protein with other transcriptional regulators. (wikipedia.org)
Among the regulators, only SP1 displayed significantly increased DNA- binding activity following DFP treatment in a dose-dependent manner. (wikipedia.org)
We observe several master transcription regulators (e.g. (biomedcentral.com)
As master transcriptional regulators, Hox proteins recognize DNA through the homeodomain (HD) and interact with a multitude of proteins, including general transcription factors and other cofactors. (biomedcentral.com)

In the SV40 virus, Sp1 binds to the GC boxes in the regulatory sequence of the genome. (wikipedia.org)
The HIV genome contains the HIV LTR with upstream DNA regulatory elements that serve as binding sites for cellular transcription factors. (brainstorming.com)
These novel interactions open the possibility to analyze promoter function and gene expression to measure transcription factor binding dynamics at target sites throughout the genome. (biomedcentral.com)

Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria. (lookformedical.com)

Cancer cells achieve their nutrient needs by secreting Vascular Endothelial Growth Factor (VEGF) to induce new blood vessel formation within the tumor mass. (erimos.com)

Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. (wikipedia.org)
Sp1 has been used as a control protein to compare with when studying the increase or decrease of the aryl hydrocarbon receptor and/or the estrogen receptor, since it binds to both and generally remains at a relatively constant level. (wikipedia.org)
In vivo footprinting studies with dimethylsulfate (DMS) revealed eight regions of apparent protein-DNA contacts on the Xa. (oregonstate.edu)
The other regions include potential binding sites for transcription factors ATF, NF1, and a CCAAT-binding protein. (oregonstate.edu)
Of particular interest are the protein interactions that occur between Hox proteins and general transcription factors (GTF) from the RNA Pol II basal transcription machinery. (biomedcentral.com)
In vivo verification was shown by a 70% reduction in GIP-R protein expression in islets from PANC PPARγ −/− mice and a twofold increase in islets of 14-day post-60% Px Sprague-Dawley rats that hyperexpress β-cell PPARγ. (diabetesjournals.org)
Transforming the gene sequence on DNA to an RNA message (a process called transcription) that can then be converted to protein is essential to the regulation of protein production. (erimos.com)

In conclusion, the SP1/miR-4757/IGF1 axis, which is regulated by LINC01376 in NPC deterioration and metastasis, is expected to provide new insights into the molecular mechanism of NPC carcinogenesis. (iubmb.org)
Collectively, above results suggested that NCTD might prevent RIF via targeting the Sp1/Gm26669 axis, thus providing a new theoretical basis for the clinical application of NCTD in the treatment of RIF. (ijbs.com)

SP1 and YAP1 mRNAs are direct targets of miR-375-5p. (wikipedia.org)
As a result, context-specific roles of transcription factors as well as their functional targets are readily explored. (biomedcentral.com)
REGNET is a useful tool for exploring the ternary relationships among the transcription factors, their functional targets and the corresponding cell conditions. (biomedcentral.com)
One main reason for the difficulty is the dynamic nature of the networks: Transcription factors (TFs) not only regulate different targets depending on the cell conditions, but their effects on these targets can also change. (biomedcentral.com)
IFI16 Targets the Transcription Factor Sp1 to Suppress HIV-1 Transcription and Latency Reactivation. (nih.gov)

TMX treatment enhanced efficacy of TRA-8 therapy on tumorigenesis in vivo . (oncotarget.com)

In contrast to monocytes, human macrophages did not show increased tissue factor expression upon stimulation with lipopolysaccharide and interferon-γ. (haematologica.org)

Can we develop a cellular nano-sensing mechanism to specifically detect and target viral markers (NA and proteins) to fight viral infections in vivo? (brainstorming.com)
Using nanotechnology and biosensor technology simultaneously, could we similarly engineer a target group of cells/tissues in vivo to house a biosensor that can detect the presence of viral nucleic acid (or any other viral component)? (brainstorming.com)
So, the scope (and intent) of this session is to try and accumulate overarching principles and methods that can be used to create an in vivo system for a nano-sensing mechanism that can detect and potentially ward off the infection by targeting the viral Nucleic Acids. (brainstorming.com)
Several viral proteins have been shown to play an important role in tumor progression by modulating transcription factors. (biomedcentral.com)

Several functional transcription factor binding sites, including MAZ and SP1 recognition sequences, have been identified. (ox.ac.uk)
The core promoter consists of SP1 binding sites, an efficient TATA element, and a highly active initiator sequence. (brainstorming.com)
Using a composite gene-set analysis method, we combine the information of transcription factor binding sites, Gene Ontology or pathway gene sets and gene expression fold-change profiles for a variety of cell conditions. (biomedcentral.com)

The Xi did not show any specifically protected sequences, and with the exception of four hyperreactive sites, the in vivo DMS reactivity profile of Xi DNA was very similar to that of purified, linear Xi DNA. (oregonstate.edu)
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)

SP1 and GATA3) and HDAC2-GATA1 regulatory networks on rescued regions in K562 cell line. (biomedcentral.com)
Here, employing an inducible B cell expression system, we have determined that M2 activates the NFAT pathway in a Src kinase-dependent manner - leading to induction of the plasma cell-associated transcription factor, Interferon Regulatory Factor-4 (IRF4). (prelekara.sk)

These results indicate that Rb can function as a positive regulator of transcription and that Sp1 is one potential target, either directly or indirectly, for transcriptional regulation by Rb. (umn.edu)
These experiments indicated that Tα3-Tα4 collaborates with factors bound to Tα1-Tα2 for the strict developmental regulation of Tcra rearrangement. (aai.org)

In vivo experiments show that ablation of Arl8b decreases IR-induced invasive tumor growth and distant metastasis. (tmu.edu.tw)

We have shown that the transcription factor Sp1 can bind to and stimulate transcription from the retinoblastoma control element motif. (umn.edu)
Our bioinformatics analysis indicated that special protein1 (Sp1), a transcription factor, may bind to the promoter of Gm26669. (ijbs.com)

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. (lookformedical.com)

Withaferin A suppresses the expression of vascular endothelial growth factor in Ehrlich ascites tumor cells via Sp1 transcription" (PDF). (wikipedia.org)

Epigenetic modifications are also strong determinants, with the factor-bound segments highly enriched for H3K4me1 and depleted of H3K27me3. (jamestaylor.org)

Combining motifs to pair a WGATAR motif with a binding site motif for GATA1, EKLF or SP1 improves discriminative power. (jamestaylor.org)

Classical polarization is usually associated with a proinflammatory response, including the increased production of tumor necrosis factor (TNF)-α, IL-1, and IL-6. (haematologica.org)
Proteoglycan degradation contributing to the pathogenesis of intervertebral disc (IVD) degeneration is induced by inflammatory cytokines, such as tumor necrosis factor‑α (TNF‑α) and interleukin‑1β (IL‑1β). (spandidos-publications.com)
Inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), are highly expressed in degenerative IVDs and contribute to a degenerative IVD phenotype by inhibiting the production of ECM ( 3 , 4 ). (spandidos-publications.com)

In agreement, using additive transgenesis to drive LacZ expression in vivo, CNS specific reporter gene expression was found with these longer constructs. (ox.ac.uk)
The factors responsible for the postnatal and starvation-associated induction of HMGCS2 gene expression have been identified and shown to involve changes in circulating hormone and fatty acid levels. (aacrjournals.org)
We propose an approach for mining context-specific transcription networks from a large collection of gene expression fold-change profiles and composite gene-set information. (biomedcentral.com)

This TF induction was dependent on signal transducer and activator of transcription-6 signaling and poly ADP ribose polymerase activity. (haematologica.org)

Genetic deficiency of NADPH oxidase does not diminish, but rather enhances, LPS-induced acute inflammatory responses in vivo. (oregonstate.edu)
Copper chelation by tetrathiomolybdate inhibits lipopolysaccharide-induced inflammatory responses in vivo. (oregonstate.edu)

Thus, H3K9me3 at Rac1 promoter assists in active DNA methylation-hydroxymethylation, activating Rac1 transcription. (nature.com)

DNA sequence motifs and epigenetic modifications contribute to specific binding by a transcription factor, but the extent to which each feature determines occupancy in vivo is poorly understood. (jamestaylor.org)

Hence, these specific elements can be targeted by the nano biosensor in vivo, potentially cleansing the cells that harbor these proviral DNA. (brainstorming.com)
Transient transfection experiments showed that the CD3 γ promoter was still active in CD3 - HTLV-I infected cells demonstrating that adequate amounts of the required transcription factors were available. (biomedcentral.com)

Terameprocol is a transcription inhibitor. (erimos.com)

However, it is becoming increasingly clear that these factors and their receptors are also widely found to express across many different cancers with further research. (medsci.org)

Finally, insulin-like growth factor 1(IGF1) was identified as the target of miR-4757. (iubmb.org)

There is strong evidence that inherited genetic factors (mutations plus single nucleotide polymorphisms) can play a major role in breast cancer susceptibility [ 1 ]. (hindawi.com)

Alternative polarization with interleukin-4 and IL-13 led to a macrophage phenotype characterized by increased tissue factor (TF) production and release and by an increase in extracellular vesicle production. (haematologica.org)

Here, we focused on the interplay between Antp and the basal transcription machinery and show the direct interaction of Antp with TBP through the poly-glutamine (PolyQ) regions of both proteins. (biomedcentral.com)

We then collected all the significant patterns and constructed a database of context-specific transcription networks for human ( REGNET ). (biomedcentral.com)

Our previous studies demonstrated that NCTD could effectively prevent RIF both in vivo and in vitro [ 8 - 11 ]. (ijbs.com)

Anatomy15

Organisms8

Chemicals and Drugs137

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes70

Disciplines and Occupations1

Information Science4

Regulates5

Vitro and in vivo4

Genes6

Putative1

Chromatin4

Knockdown2

Colorectal cancer2

Mechanistically1

Promoter region3

MRNA levels1

Zinc finger transcription factor1

Genomic2

Nuclear5

Promoters2

Regulators4

Genome3

Cytoplasmic1

Induce1

Protein7

Axis2

Targets5

Tumorigenesis1

Monocytes1

Viral4

Binding sites3

Sequences2

Regulatory2

Regulation2

Decreases1

Bind2

Initiation1

Vascular endoth1

Bound1

Motif1

Tumor necros3

Expression3

Activator1

Inflammatory2

Methylation1

Epigenetic modifications1

Cells2

Inhibitor1

Receptors1

Target1

Breast cancer1

Increase1

Poly1

Human1

Prevent1