Loading...
  • cellular
  • The snapshots of these polymers captured using cryo-EM will continue to provide critical insights into their dynamics, interactions with cellular components, and the way microtubules contribute to cellular functions in diverse physiological contexts. (biochemistry.org)
  • dynamic
  • The development of cryo-electron microscopy (cryo-EM) allowed microtubules to be captured in their solution-like state, enabling decades of insight into their dynamic mechanisms and interactions with binding partners. (biochemistry.org)
  • Although work is ongoing to define a complete structural model of dynamic instability, attention has focused on the role of gradual destabilization of lateral contacts between tubulin protofilaments, particularly at the microtubule seam. (biochemistry.org)
  • Second, we will use our unique capacity to perform dynamic micropatterning, to add or remove actin nucleation sites in real time, in order to investigate the ability of dynamic networks to adapt to changes and the role of coupled network dynamics in this emergent property. (europa.eu)
  • mechanisms
  • By examining the mechanisms these bacteria use to communicate with the host cell cytoskeleton, we hope to identify new ways to interfere with the infection process, and arrive at a deeper understanding of the normal regulation of cytoskeletal shape changes and cell movement. (stanford.edu)
  • cells
  • Thus, acentrosomal microtubule nucleation may be a key feature during differentiation of neuronal, but also of non-neuronal cells. (uni-muenchen.de)
  • In plant cells, by contrast, microtubules initiate from dispersed sites in the cell cortex, where they then self-organize into parallel arrays. (jove.com)
  • Since microtubules (MTs) were first observed in cells [ 1 , 2 ] and tubulin was first purified and proposed to be the building block of MT [ 3 ], electron microscopy (EM) has been a crucial technique for investigating MT molecular mechanism and functional context in cells and tissues. (biochemistry.org)
  • However
  • Strikingly, however, the combination of golgins conserved among diverse eukaryotes allows for the most detailed reconstruction of the organelle to date, showing a sophisticated Golgi with differentiated compartments and trafficking pathways in the common eukaryotic ancestor. (beds.ac.uk)
  • provide
  • Cryo-EM micrographs provide 2D visualization of microtubules, and these 2D images can also be used to reconstruct the 3D structure of the polymer and any associated binding partners. (biochemistry.org)
  • control
  • In vitro reconstituted systems will be used to control the actin nucleation patterns, the closed volume of the reaction chamber and the physical interaction of the networks. (europa.eu)
  • Development
  • Using rodent hippocampal neurons in culture as a model system, I found that the centrosome loses its function as a microtubule organizing center (MTOC) during neuronal development. (uni-muenchen.de)