• Scalpel, the leading genetic variants discovery tool, to identify and analyze mutations in autism spectrum disorders and somatic mutations (or post-conception DNA alterations) in cancer genomes, including pancreatic cancers. (agbt.org)
  • In addition to entire chromosomes, most hybrids were found to contain one or a few chromosome fragments, as a result of rearrangements that had occurred in vitro. (nih.gov)
  • To assess the viability of human sperm for in vitro fertilization Somatic cell hybrids between humans and hamsters or mice have been used for the mapping of various traits since at least the 1970s. (wikipedia.org)
  • Chimeras, Hybrids, and Interspecies Research Politics and Policymaking. (wikipedia.org)
  • 1. Analysis of oncogene, tumor suppressor gene, and chromosomal alterations in HeLa x osteosarcoma somatic cell hybrids. (nih.gov)
  • 2. Modification of myc gene amplification in human somatic cell hybrids. (nih.gov)
  • 28, 1994 and entitled "Multifunctional molecular complexes for gene transfer to cells" which is incorporated herein by reference. (justia.com)
  • The transfer of genetic material into cells is the basis of a number of processes now widely accepted in the areas of molecular biology, gene therapy and genetic immunization. (justia.com)
  • 5. Role of chromosome loss in ras/myc-induced Syrian hamster tumors. (nih.gov)
  • 10. Suppression of tumorigenicity of rat liver tumor cells by human chromosome 13: evidence against the involvement of pRb and BRCA2. (nih.gov)
  • Forty additional primary hybrids, in which conventional cytogenetic analysis failed to reveal any complete human chromosome, contained many human chromosome fragments. (nih.gov)
  • Heretofore, viral vectors of various types have been successfully utilized for the insertion of selected foreign genetic information into a target cell, and in the case of eukaryotic cells, for incorporation of that genetic information into the genome of the cell. (justia.com)
  • Calls for the creation of inter-species embryos have been prompted by a shortage of human eggs required for the cloning of fully human embryos for the purpose of embryonic stem-cell research. (thinkingfaith.org)
  • If the technique were perfected, cybrid embryos would be allowed to develop for a few days, after which their stem cells would be removed for research. (thinkingfaith.org)
  • Using the cloning technique, the cell nucleus of a rabbit or cow egg would be removed and the cell nucleus of a somatic human cell, such as a skin cell, would be inserted instead. (thinkingfaith.org)
  • The new entity, called a cytoplasmic hybrid, or for short a cybrid , would house human chromosomes in its cell nucleus as well as a few animal genes in the cytoplasm left in the animal egg. (thinkingfaith.org)
  • and transport to and through the nuclear membrane into the nucleus of the cell where transcription of the genetic material can take place. (justia.com)
  • The data suggest that, at low pH, diphtheria toxin (or its A fragment) penetrates directly through the surface membrane of the cell. (rupress.org)
  • Human chromosomes implicated as suppressors of tumorigenicity in hybrids with Chinese hamster ovary cells. (nih.gov)
  • 6. Loss of a putative tumor suppressor locus after gamma-ray-induced neoplastic transformation of HeLa x skin fibroblast human cell hybrids. (nih.gov)
  • 7. Homozygous deletions within the 11q13 cervical cancer tumor-suppressor locus in radiation-induced, neoplastically transformed human hybrid cells. (nih.gov)
  • 11. Modulation of differentiation in PA-1 human teratocarcinoma cells after N-ras oncogene-induced tumorigenicity. (nih.gov)
  • 17. Loss of suppressor loci on chromosomes 11 and 14 may be required for radiation-induced neoplastic transformation of HeLa x skin fibroblast human cell hybrids. (nih.gov)
  • 19. The human breast carcinoma cell line SW 613-S: an experimental system to study tumor heterogeneity in relation to c-myc amplification, growth factor production and other markers (review). (nih.gov)
  • DNA samples from about 100 human-hamster somatic cell hybrids, previously characterized by conventional banding techniques, were amplified with dual-Alu PCR. (nih.gov)
  • The products were then used as probes in FISH experiments on normal human metaphases for an accurate cytogenetic characterization of the human material retained in each hybrid. (nih.gov)
  • A humster is a hybrid cell line made from a hamster oocyte fertilized with human sperm. (wikipedia.org)
  • Humsters are routinely created mainly for two reasons: To avoid legal issues with working with pure human embryonic stem cell lines. (wikipedia.org)
  • Hamster zona-free ovum test Human-animal hybrid Recombinant DNA Yanagimachi, R. (wikipedia.org)
  • I have reviewed and submitted formal analyses on many national and international legislations on this and related issues [e.g., see my analysis of the Canadian CIHR stem cell report, the British House of Lords cloning bill, the U.S. human cloning bills, etc. (lifeissues.net)
  • Hybrids between dwarf hamsters Phodopus sungorus and P. campbelli provide a good model for studies in cytological and genetic mechanisms of hybrid sterility. (mdpi.com)
  • The present invention is in the field of methods for the transfer of genetic information, e.g., foreign DNA, into target cells, especially eukaryotic cells. (justia.com)
  • Transfer of the genetic information encoded in DNA to cells where it expresses identified individual proteins, has permitted investigation of the function of such proteins on a cellular level, and of the underlying cell physiology. (justia.com)
  • Genetic material has also been transferred into cells to introduce proteins that are absent due to an inherent genetic flaw in the cell that expresses an inactive protein or else prevents expression of the protein altogether. (justia.com)
  • The transfer of genetic material into cells can be used to prevent the expression of proteins in those cells through the well-known antisense effect of complementary DNA or RNA strands. (justia.com)
  • Exogenous, i.e., foreign genetic material can permit cells to synthesize significant amounts of proteins that are not available by other means in practical economic terms. (justia.com)
  • The in vivo introduction of exogenous genetic material into cells also has potential utility in applications for the alleviation, treatment or prevention of metabolic, tumoral or infectious disorders by the same mechanisms enumerated above. (justia.com)
  • 20. A functional genomics approach for the identification of putative tumor suppressor genes: Dickkopf-1 as suppressor of HeLa cell transformation. (nih.gov)
  • Homeotic selector genes also may be determinants of cell compartments-i.e., they may regulate genes that encode molecules that enable cells to mix only with cells in the same compartment. (nih.gov)
  • Upon a short incubation with diphtheria toxin at low pH, the rate of protein synthesis in the cells decreased much faster than when the normal pH was maintained. (rupress.org)
  • When, to cells preincubated with diphtheria toxin and NH4Cl, neutralizing amounts of anti-diphtheria toxin were added before the pH was lowered, the toxic effect was considerably reduced, but it was not completely abolished. (rupress.org)
  • In addition, cells in the hindbrain of the developing chick embryo do not cross rhombomere boundaries (7), which suggests that rhom- bomeres correspond to cell compartments in the developing The publication costs of this article were defrayed in part by page charge payment. (nih.gov)
  • A multifunctional molecular complex for the transfer of a nucleic acid composition to a target cell is provided. (justia.com)