Somatic cell donor. Medical search. Frequent questions

Successful somatic-cell nuclear transfer (SCNT) depends on the quality, availability and maturation of the animal's unfertilized oocytes. (nature.com)
For SCNT, the chromosomes of the unfertilized canine oocytes were removed by micromanipulation, and a single donor cell was transferred into each enucleated oocyte. (nature.com)
In genetics and developmental biology, somatic cell nuclear transfer (SCNT) is a laboratory strategy for creating a viable embryo from a body cell and an egg cell. (wikipedia.org)
In human SCNT experiments, these eggs are obtained through consenting donors, utilizing ovarian stimulation. (wikipedia.org)
Controversy surrounds human ESC work due to the destruction of viable human embryos, leading scientists to seek alternative methods of obtaining pluripotent stem cells, SCNT is one such method. (wikipedia.org)
For example, if a person with Parkinson's disease donated their somatic cells, the stem cells resulting from SCNT would have genes that contribute to Parkinson's disease. (wikipedia.org)
Another application of SCNT stem cell research is using the patient specific stem cell lines to generate tissues or even organs for transplant into the specific patient. (wikipedia.org)
Only a handful of the labs in the world are currently using SCNT techniques in human stem cell research. (wikipedia.org)
Somatic cell nuclear transfer (SCNT) is a type of cloning that has to be done in a lab. (bartleby.com)
In SCNT they take the nucleolus out of an egg cell, replace it with the nucleolus of a somatic cell (body cell with two complete sets of chromosomes), and make the egg cell divide into a blastocyst ("What Is Cloning? (bartleby.com)
One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
This method is called "somatic cell nuclear transfer" or SCNT. (scienceblog.com)
Indeed, if passed, Hatch/Feinstein/Kerry would explicitly legalize doing in humans the very cloning procedure -- somatic cell nuclear transfer (SCNT) -- that was used to make Dolly the sheep . (lifeissues.net)
The team led by Galina Singina at the Ernst Federal Science Center for Animal Husbandry managed to clone the calf using somatic cell nuclear transfer (SCNT), with embryonic fibroblasts as donors of nuclei. (isaaa.org)
Analysis of the effectiveness of caffeine and MG132 for improving somatic cell nuclear transfer (SCNT) technology using cryopreserved eggs showed that supplementation did not improve the blastocyst formation rate of cloned mouse eggs. (molcells.org)
The promise of the SCNT method is that the nucleus of a patient's skin cell, for example, could be used to create pluripotent cells that might be able to repair a part of that patient's body. (pharmaceuticalintelligence.com)
One attraction of SCNT has always been that the genetic identity of the new pluripotent cell would be the same as the patient's, since the transplanted nucleus carries the patient's DNA. (pharmaceuticalintelligence.com)
Stanford University have raised the possibility in the past that the immune system of a patient who received SCNT-derived cells might still react against the cells' mitochondria, which act as the energy factories for the cell and have their own DNA. (pharmaceuticalintelligence.com)
This reaction could occur because cells created through SCNT contain mitochondria from the egg donor and not from the patient, and therefore could still look like foreign tissue to the recipient's immune system. (pharmaceuticalintelligence.com)
When transplanted back into the nucleus donor strain, the cells were rejected although there were only two single nucleotide substitutions in the mitochondrial DNA of these SCNT-derived cells compared to that of the nucleus donor. (pharmaceuticalintelligence.com)
When scientists at the Oregon Health and Science University announced success in performing SCNT with human cells last year, it reignited interest in eventually using the technique for human therapies. (pharmaceuticalintelligence.com)
Although many stem cell researchers are focused on a different method of creating pluripotent stem cells, called induced pluripotent stem cells, there may be some applications for which SCNT-derived pluripotent cells are better suited. (pharmaceuticalintelligence.com)
The immunological reactions reported in the new paper will be a consideration if clinicians ever use SCNT-derived stem cells in human therapy, but such reactions should not prevent their use. (pharmaceuticalintelligence.com)
This research informs the medical community of the margin of safety that would be required if, in the distant future, researchers need to use SCNT to create pluripotent cells to treat someone. (pharmaceuticalintelligence.com)

The technique consists of taking an denucleated oocyte (egg cell) and implanting a donor nucleus from a somatic (body) cell. (wikipedia.org)
Each of the 11 new human embryonic stem cell lines was created by transferring the nuclear genetic material from a non-reproductive cell of a patient into a donated egg, or "oocyte," whose nucleus had been removed. (scienceblog.com)
Oocyte donors and patients who donated non-reproductive cells were all unpaid volunteers. (scienceblog.com)
In a Science "Policy Forum" related to the team's latest findings, David Magnus and Mildred Cho from Stanford University in Palo Alto, CA discuss international oversight and ethical issues in oocyte donation, including the need to promote realistic expectations of the outcomes of stem cell research. (scienceblog.com)
The single cell line generated in the 2004 Science paper resulted from nuclear transfer in which the oocyte and non-reproductive ("somatic") cell came from the same healthy female. (scienceblog.com)
Photographs of (a) the procedure of somatic cell transfer (shown with the arrow) into the perivitelline space of an enucleated oocyte, (b) cloned bovine embryos used for transplantation to recipient animals, and (c) cloned calf (obtained in Russia for the first time). (isaaa.org)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
To do this, DNA is first taken from a donor oocyte (egg cell). (bifconference.com)
Then the diploid nucleus from the edited somatic cell is moved into the oocyte and implanted into a surrogate dam, said Van Eenennaam. (bifconference.com)
Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
Dr. Campbell and Sir Ian obtained U.S. Patent No. 7,514,258 for the method they used to produce Dolly: somatic cell nuclear transfer, which involves removing the nucleus of a somatic cell that has been arrested in the quiescent phase of the cell cycle and implanting that nucleus into an enucleated oocyte. (patentdocs.org)
Galli, C. (2005), Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type. (rfgi.fr)

The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
In their new paper, Science author Woo Suk Hwang from Seoul National University in Seoul, Korea and colleagues replaced the nuclei from donated oocytes with nuclei from skin cells from male and female patients, ranging in age from 2 to 56, who had spinal cord injuries, juvenile diabetes and the genetic disease "congenital hypogamma-globulinemia. (scienceblog.com)
These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
These observations suggest that further studies on nuclear reprogramming are needed in order to understand the underlying mechanisms of reprogramming and significantly improve the ability of the differentiated somatic nuclei to be reprogrammed. (biomedcentral.com)
Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. (cnrs.fr)
Granulosa cells from adult sheep were heated to nonphysiological temperatures (55 degrees C or 75 degrees C) before their nuclei were injected into enucleated metaphase II oocytes. (cnrs.fr)
In addition, our experiments show that heat-treating donor nuclei destabilizes higher-order features of chromatin (but leaves intact its nucleosomal organization) and results in a high proportion of reconstructed embryos developing to the blastocyst stage and beyond. (cnrs.fr)
Li X, Morris LHA & Allen WR (2002) In vitro development of horse oocytes reconstructed with the nuclei of fetal and adult cells. (rfgi.fr)
The team used cells that were created by transferring the nuclei of adult mouse cells into enucleated eggs cells from genetically different mice. (pharmaceuticalintelligence.com)
95 longitudinal ridges on the external cuticle, 2-5 chord had significantly higher OD values for total serum nuclei per section, and robust muscle cells, all of which are immunoglobulin G against D . repens somatic antigen characteristic features of D . repens ( 10 ). (cdc.gov)

Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo . (nature.com)
The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)
The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
From the 185 donated oocytes, endowed with the genetic material from a different person (or in one case, the same person), the researchers report development of 31 hollow balls of cells called "human nuclear-transfer blastocysts. (scienceblog.com)
The ten additional new lines resulted from nuclear transfer with skin cells of males or females and oocytes from biologically-unrelated females. (scienceblog.com)

Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
To our knowledge, this is the first report of cloned mammalian offspring originating from nonviable cells. (cnrs.fr)

These DNA mistakes are also termed somatic mutations, meaning mutations that arise once the body is already formed (soma is the ancient Greek word for body). (alleninstitute.org)

These cells are deemed to have a pluripotent potential because they have the ability to give rise to all of the tissues found in an adult organism. (wikipedia.org)
Embryonic stem cells are immortal, and have the potential to develop into any type of adult cell, even after months growing in culture dishes. (news-medical.net)
By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
The term stem cell is also used in reference to any adult cells that are capable of assisting in the restoration of adult tissue via self-renewal. (citizendium.org)
Such tissue renewal may be accomplished via the use of adult stem cells, or embryonic stem cells, which may be derived from a human embryo in the blastocyst stage. (citizendium.org)
Adult stem cells hold great promise in mitigating much of the ethical debate over embryonic stem cell use. (citizendium.org)
B ) Histological cross section through an adult mouse testis depicting seminiferous tubules, the peritubular myoid cells, and the interstitium (space between tubules). (jci.org)
The inventors on the '233 application, Dr. Keith H.S. Campbell and Sir Ian Wilmut, were the first to produce a cloned mammal from an adult somatic cell -- Dolly the sheep. (patentdocs.org)
Looking at the mutations that crop up when we are just two cells big, the researchers found that those cells don't always evenly contribute to our adult bodies. (alleninstitute.org)
CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. (thermofisher.com)
Adult Stem Cells - occurring in adult organisms. (orthodoxwiki.org)
In some adult tissues, such as bone marrow, muscle, and brain, discrete populations of adult stem cells generate replacement cells. (orthodoxwiki.org)
Any of the cells resulted from these divisions can give rise to all the cells needed to make up an adult organism. (orthodoxwiki.org)
Adult stem cells give the body its ability to repair and replace the cells and tissues of some organs. (orthodoxwiki.org)
Adult stem cells are rare, and their origin in mature tissue is not yet completely understood. (orthodoxwiki.org)
Adult stem cells are dispersed in tissues throughout the mature organism and behave very differently depending on the local environment. (orthodoxwiki.org)
Some recent studies focus on the plasticity of the adult stem cells, which is the ability to differentiate in specialized cells of another tissue. (orthodoxwiki.org)
Specialized iPSCs are reprogrammed from adult skin or infant cord blood cells and can become any cell type in the body - a condition called pluripotency that mimics the function of human embryonic stem cells (hESCs). (neurosciencenews.com)
The cells were generated with a variety of genes, methods and cells of origin, such as adult skin or infant cord blood cells. (neurosciencenews.com)
Embryotic, Adult, and induced pluripotent stem cells. (web.app)
It also specifies that " the principle of anonymity of the donation is not an obstacle for the adult child " to access some non identifying data about the donor. (genethique.org)
One method of creating pluripotent stem cells is called somatic cell nuclear transfer, and involves taking the nucleus of an adult cell and injecting it into an egg cell from which the nucleus has been removed. (pharmaceuticalintelligence.com)
GreggsEng 101-0214/10/08Research Contains Adult Stem Cells, No Embryos AllowedStem cell research has been argued about for quite some time now. (freeessays.education)
Stem cells may be embryonic or adult, somatic. (freeessays.education)
Unfortunately, most people have not been informed of another kind of stem cell research: Adult (somatic) stem cell research. (freeessays.education)
It is called somatic (adult) stem cell research. (freeessays.education)
Somatic stem cells are adult cells that are used to replace and replenish cells that are continually lost by depletion and damage. (freeessays.education)
One of the ways that makes somatic stem cells different from embryonic stem cells is that somatic stem cells are from adult human beings. (freeessays.education)
There is no proven technique to turn adult stem cells into any other cell other than their tissue of origin, to date. (freeessays.education)
The disadvantages of adult stem cell research are, unlike embryonic stem cells, they cannot manipulate the cell to any specificity. (freeessays.education)

Somatic cell nuclear transplantation has become a focus of study in stem cell research. (wikipedia.org)
This could allow us to create cells that are useful for transplantation for a variety of diseases without the problem of immunological rejection," said Noggle in a press briefing. (the-scientist.com)
Cross-species transplantation was possible without the rejection of the human embryonic stem cells by the mice's immune systems because the mice were genetically modified to suppress certain immune responses that would have interfered with transplantation. (citizendium.org)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Because the extent of previous transfusion has been shown to significantly affect the outcomes of patients undergoing hematopoietic cell transplantation (HCT) for aplastic anemia, the rapidity with which these data are obtained is crucial. (medscape.com)
The use of human stem cell-based models, including human brain organoids and cell transplantation, will further the efforts of the scientific community to unravel the complexity of dopamine neurons, driving advancements in stem cell-based treatments in Parkinson´s disease," revealed Alessandro. (lu.se)
The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
Relationship between certain HLA alleles and the risk of cytomegalovirus reactivation following allogeneic hematopoietic stem cell transplantation. (cdc.gov)
Sex XY Ethnic origin Caribbean Comment A haploidentical bone marrow transplantation, with the Comment mother as the donor, was performed when the patient was 30 Comment months old. (lu.se)
Central venous catheter placement is required before the administration of hematopoietic cell transplantation. (medscape.com)

Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
If tissues and cells are being used as starting materials in a medicinal product, the donation, procurement and testing of the cells are covered by the Tissues and Cells Directive (2004/23/EC) . (ravimiamet.ee)
Tissue establishments supplying such materials are required to have a system in place to trace all substances coming into contact with the cells and tissues while maintaining donor and patient confidentiality. (ravimiamet.ee)
Dec. 29, 2005 -- South Korean laboratories used by scientist Hwang Woo Suk no longer have any stem cells created from patients' tissues, the result of the researcher's landmark May 2005 paper, the Seoul National University said. (blogspot.com)
The Somatic Mosaicism across Human Tissues (SMaHT) Network aims to transform our understanding of how somatic mosaicism in human cells influences biology and disease. (nih.gov)
To realize its goal, the SMaHT Network will systematically document DNA sequence variants within personal genomes by detecting DNA variation in tissues from human donors using state-of-the art sequencing technologies. (nih.gov)
Somatic Variant Discovery: This initiative will generate a catalog of human somatic variation by sequencing data from 10-15 sets of tissues collected from 150 individuals. (nih.gov)
Cells of the same type make tissues, and tissues make organs. (orthodoxwiki.org)
CHICAGO HIGH SCHOOL FOR AGRICULTURAL SCIENCES Stem Cell Research: Breast Cancer The Nature of Stem cell research - RationalWiki Stem cell research proposes to use stem cells to repair or replace damaged tissues in order to treat an extremely wide range of conditions, including severed spinal cords, non-functioning endocrine glands, diabetes, cancer, heart disease, various types of brain damage, and many other conditions. (web.app)
Mouse cells and tissues created through nuclear transfer can be rejected by the body because of a previously unknown immune response to the cell's mitochondria, according to an international study in mice by researchers at the Stanford University, MIT and colleagues in Germany and England. (pharmaceuticalintelligence.com)
The hope has been that this would eliminate the problem of the patient's immune system attacking the pluripotent cells as foreign tissue, which is a problem with most organs and tissues when they are transplanted from one patient to another. (pharmaceuticalintelligence.com)

We also molecularly defined the development of Sertoli, Leydig and peritubular myoid cells during the perinatal period, allowing us to identify candidate signaling pathways acting between somatic and germ cells in a stage-specific manner during the perinatal period. (biologists.com)
Totipotent cells have the capacity to differentiate to all cell types, including somatic cells, germ cells, and certain cells that exist outside the embryo and are important to fetal development that are termed extraembryonic cells. (citizendium.org)
In this Review, we briefly outline the roles that follicle-stimulating hormone (FSH) and testosterone play in regulating spermatogenesis and describe our current understanding of how vitamin A regulates germ cell differentiation and how it may lead to the generation of both the cycle of the seminiferous epithelium and the spermatogenic wave. (jci.org)
Stem cells originating in human embryos can be categorized as either embryonic stem cells or embryonic germ cells . (orthodoxwiki.org)
Embryonic germ cells share many of the characteristics of the embryonic stem cells but differ in significant ways. (orthodoxwiki.org)
They are derived from the primordial germ cells, which occur in a specific part of the embryo/fetus called the gonadal ridge. (orthodoxwiki.org)
Germ cells do not proliferate as long as stem sells. (orthodoxwiki.org)
Under certain conditions, germ cells do differentiate into specialized cells. (orthodoxwiki.org)
Germ cells and stem cells also differ with respect to their growth characteristics in vitro and their behavior in vivo. (orthodoxwiki.org)
Teratomas, benign tumors containing the developing cells of different body parts, allowed researchers to see if the iPSCs could form the body's three basic germ cell lines - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
Being pluripotent means iPSCs can generate cells from each of the three basic germ cell lines that form the body - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)

They have lost the ability to differentiate to all cell types needed for a complete embryo development (up to 14 days post-fertilization). (orthodoxwiki.org)

Donor fibroblasts were obtained from an ear-skin biopsy of a male Afghan hound and cultured for two to five passages (in which fully grown cells are transferred to a new culture dish). (nature.com)
Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)

Snuppy is genetically identical to the donor Afghan hound. (nature.com)

Furthermore, the use of diphtheria toxin -- which is far more toxic to human cells than mouse cells -- to destroy the human neurons in the mice reversed the observed improvements in motor function. (citizendium.org)
This result suggests that the observed increase in motor function was indeed produced by neurons derived from the human embryonic stem cells. (citizendium.org)
Although his research in this area originally focused on brain disease - some forms of epilepsy are linked to somatic mutations in neurons - he soon realized these mutations also served as coded histories of our past. (alleninstitute.org)
under certain conditions, they can be induced to become cells with special functions (like muscle cells, liver cells, neurons, etc. (orthodoxwiki.org)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

The advance here is the proof that somatic cell nuclear transfer can work [in human cells] and can fully reset the donor cell genome to a pluripotent state," said Harvard Medical School's George Daley , who was not affiliated with the study. (the-scientist.com)
Noggle said the findings may also pave the way for better induced pluripotent stem cells (iPSCs), which form when somatic cells are regressed to a pluripotent state through the use of genetic factors. (the-scientist.com)
No. 184, Counterclaims ¶ 43 ("iPSCs are generated in culture from somatic cells through the introduction of reprogramming factors that transform a somatic cell into a pluripotent state. (justia.com)

Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning the clone embryo is implanted into a host mother for further development and brought to term. (wikipedia.org)
Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
One of the next preclinical steps, according to the authors, is to evaluate, in the lab, differentiated patient-specific human embryonic stem cell lines for immune-system tolerance, therapeutic efficacy and safety. (scienceblog.com)
A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
The findings validate this controversial method, and may one day allow therapeutic stem cells to be created from a patient's own genetic material. (the-scientist.com)
In the best case, an early embryo consisting of a few cells may form, but these are not capable of giving rise to human life, nor hESCs for therapeutic purposes. (the-scientist.com)
The triploid cells aren't suitable for therapeutic purposes, and future efforts will be focused on trying to eliminate the [egg cell] genome," said Daley, who wrote an accompanying News & Views in Nature . (the-scientist.com)
While iPSCs avoid the ethical issues surrounding embryonic stem cells, the methods used to derive them sometimes induce mutations in cancer causing genes, making them unsuitable for therapeutic purposes. (the-scientist.com)
The use of the pluripotent and/or self-renewing qualities of stem cells is believed to have therapeutic benefits for the regeneration of tissue in humans. (citizendium.org)
Human embryos fertilized in the ordinary manner and harvested in the blastocyst stage have been used as an extensive source of stem cells for research purposes, and have been shown to possess therapeutic value in laboratory animals. (citizendium.org)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)

By cataloging the extent of somatic mosaicism in different cell types, disease states, and life stages, the SMaHT Network will lead to new understandings of how much somatic mosaicism influences fetal development, disease processes, and aging. (nih.gov)
Hemoglobin electrophoresis and blood-group testing may show elevated levels of fetal hemoglobin (HbF) and red cell I antigen, suggesting stress erythropoiesis. (medscape.com)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)

We characterize the landscape of somatic mutations-mutations occurring after fertilization-in the human brain using ultra-deep (~250×) whole-genome sequencing of prefrontal cortex from 59 donors with autism spectrum disorder (ASD) and 15 control donors. (nih.gov)

In the United States, scientists at the Harvard Stem Cell Institute, the University of California San Francisco, the Oregon Health & Science University, Stemagen (La Jolla, CA) and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce embryonic stem cells. (wikipedia.org)

In the end, the egg cell contained three sets of chromosomes-two from the diploid somatic cell, and one from the haploid egg. (the-scientist.com)

Cell-based medicinal products cover a wide range of cell therapies, including somatic cell and tissue-engineered products that have been manufactured from a patient's own cells (autologous), donor cells (allogeneic) or animal cells (xenogeneic). (ravimiamet.ee)
The article 7 of the bill specifies that the donation of cord blood and placenta blood cells can only be an anonymous donation for an allogeneic usage: the creation of blood cord autologous private banks is thus de facto prohibited, what regret some people like Pierre Le Coz, vice-chairman of the National Consultative Ethics Committee (CCNE). (genethique.org)
The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

this approach has been championed as an answer to the many issues concerning embryonic stem cells (ESCs) and the destruction of viable embryos for medical use, though questions remain on how homologous the two cell types truly are. (wikipedia.org)
Embryonic stem cells are derived from early embryos and have the ability to differentiate into any cell type. (spiked-online.com)
Then, in February 2004 he dropped a bombshell, claiming that his SNU research team had cloned the first human embryos and extracted stem cells from them. (koreatimes.co.kr)
A couple of studies show some success in generating early microscopic embryos, but this [study] is the first successful pluripotent stem cell line," said Daley. (the-scientist.com)
This could help researchers identify abnormalities in iPSC differentiation, correct them, and develop pluripotent stem cells that don't harbor tumorigenic qualities and do not require the use of human embryos. (the-scientist.com)
The use of embryonic stem cells has been a source of considerable controversy due to its sacrifice of human embryos in the blastocyst stage, which some people view as the destruction of human life . (citizendium.org)
The most infamous study of embryonic stem cells asserted that cloned human embryos had been created via somatic cell nuclear transfer, and stem cells had been generated from these embryos. (citizendium.org)
Ethical objections to the use of human embryonic stem cells revolve around the destruction of human embryos in the blastocyst stage to obtain the stem cells. (citizendium.org)
A new study traces human development from adults all the way back to embryos when they were just a few cells. (alleninstitute.org)
The center's director, Christopher Walsh , M.D., Ph.D., and other scientists at Harvard Medical School and Boston Children's Hospital used those mutations to decode the early history of human development, looking all the way back to eight-cell and even two-cell embryos. (alleninstitute.org)
The eggs will then be used by scientists from Newcastle and Durham Universities to create embryos from which they will attempt to derive stem cells . (progress.org.uk)
In Vitro Fertilization - some of the embryos used in human stem cells research were initially created for infertility purposes through in vitro fertilization procedures. (orthodoxwiki.org)

9 Blood centers are actively urging healthy donors to contribute, bearing in mind that only 3% of eligible Americans donate blood. (cap.org)
This raises the possibility, for example, that the existing data will overstate the potential risks for healthy donors, given that IVF patients may be more likely to have a variety of conditions, such as pelvic adhesions and polycystic ovary syndrome, that increase the odds of complications from the ovarian stimulation or the retrieval surgery. (nationalacademies.org)
Validation studies in a large number of healthy donors is necessary in order to make any claim of this blood-based test's detection for colon cancer," said Beg, director for gastrointestinal medical oncology at the University of Texas Southwestern Medical Center, Dallas. (medscape.com)

This gives them the ability to create patient specific pluripotent cells, which could then be used in therapies or disease research. (wikipedia.org)
These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
3 Today, serologic and molecular techniques, along with laboratory information systems and electronic health records, contribute to precise blood product management and personalized transfusion therapies, particularly benefiting complex patients with sickle cell disease, thalassemia, and other diseases requiring chronic transfusion support. (cap.org)
As the promise of using regenerative stem cell therapies draws closer, a consortium of biomedical scientists reports about 30 percent of induced pluripotent stem cells they analyzed from 10 research institutions were genetically unstable and not safe for clinical use. (neurosciencenews.com)
It was very surprising to us the high number of unstable cell lines identified in the study, which highlights the importance of setting safety standards for stem cell therapies," said Carolyn Lutzko, PhD, senior author and director of translational development in the Translational Core Laboratories at Cincinnati Children's Hospital Medical Center. (neurosciencenews.com)
A good number of the cell lines we studied met quality standards, although the unexpected number of lines that did not meet these standards could not be used for clinical therapies. (neurosciencenews.com)
It gives scientists open access to data from the study to support their own research into potential iPSC-based stem cell therapies. (neurosciencenews.com)
Stem cell therapies hold vast potential for repairing organs and treating disease. (pharmaceuticalintelligence.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

a , Snuppy, the first cloned dog, at 67 days after birth (right), with the three-year-old male Afghan hound (left) whose somatic skin cells were used to clone him. (nature.com)
Development will ensue normally and after many mitotic divisions, the single cell forms a blastocyst (an early stage embryo with about 100 cells) with an identical genome to the original organism (i.e. a clone). (wikipedia.org)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
The resulting clone developed into a microscopic embryo, which survived long enough for pluripotent stem cell lines to be derived. (the-scientist.com)
The first is to edit a somatic cell from an elite animal and then clone that cell. (bifconference.com)
164. The clone of any of claims 155-159, wherein the donor mammal is non-foetal. (patentdocs.org)
HumanPass Wednesday confirmed fingerprinting traces of Snuppy, Hwang's canine clone, matched those of its somatic cell donor, an Afghan hound named Tai, while they demonstrated disparate mitochondrial genotypes. (blogspot.com)

When they were no longer needed for that purpose, they were donated for research with the informed consent of the donor. (orthodoxwiki.org)

The blastocyst stage is developed by the egg to help create embryonic stem cells from the inner cell mass of the blastocyst. (wikipedia.org)
Stem cells were then derived from the inner cell mass of the blastocyst. (scienceblog.com)
The Korean researchers who performed this stem cell research improved upon their protocols that yielded the first embryonic stem cell line from a cloned human blastocyst. (scienceblog.com)
However, supporters of embryonic stem cell research frequently contend that even the comparison to abortion is inappropriate, since while a several month old fetus might have sufficient neurological development to be conscious in some meaningful sense, a human embryo in the blastocyst stage has so little development that one can safely conclude that it cannot exist as a conscious being. (citizendium.org)
In the interior of the blastocyst, there is a cluster of about 30 cells called the inner cell mass. (orthodoxwiki.org)
The cells that form the inner cell mass of the blastocyst are called pluripotent stem cells . (orthodoxwiki.org)
After around five days of development blastocyst, which is a ball of up to 100 cells that do not have a specific task. (freeessays.education)
Joseph Panno says in his book titled Stem cell research medical applications and ethical controversy Ð²Ð‚ÑšThere is no way to remove cells from the ICM of a blastocyst without killing the embryoÐ²Ð‚Ñœ. (freeessays.education)

The ovum, now containing the somatic cell's nucleus, is stimulated with a shock and will begin to divide. (wikipedia.org)
The proteins produced from the JAK2 , MPL , and THPO genes are part of a signaling pathway called the JAK/STAT pathway, which transmits chemical signals from outside the cell to the cell's nucleus. (medlineplus.gov)

Somatic cell nuclear transfer is a technique for cloning in which the nucleus of a somatic cell is transferred to the cytoplasm of an enucleated egg. (wikipedia.org)
Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)
Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)
Given that we have an efficiency of 1% cloning for livestock species and if only one in a thousand cells are viable then around 100,000 cells would need to be transferred. (wikiquote.org)
It's given name is the "Human Cloning Ban and Stem Cell Research Protection Act of 2003," the stated purpose of which, supposedly, is to "prohibit human cloning and to protect important areas of medical research, including stem cell research. (lifeissues.net)
However, somatic cloning has been inefficient in all species in which live clones have been produced. (biomedcentral.com)
Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
Schematic diagram of the somatic cloning process. (biomedcentral.com)
Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)

We observe a mean of 26 somatic single-nucleotide variants per brain present in ≥4% of cells, with enrichment of mutations in coding and putative regulatory regions. (nih.gov)
This suggests that a typical individual possesses ~80 somatic single-nucleotide variants present in ≥2% of cells-comparable to the number of de novo germline mutations per generation-with about half of individuals having at least one potentially function-altering somatic mutation somewhere in the cortex. (nih.gov)

In addition, researchers must develop methods to efficiently direct the differentiation of embryonic stem cells to specific stable cell types. (scienceblog.com)
The researchers generated these stem cell lines ten times more efficiently than in their 2004 Science study, using improved laboratory methods. (scienceblog.com)
Alternatively, research using eggs may point the way to methods which mimic their properties using other human cells and chemical agents. (spiked-online.com)
The '856 Patent is entitled 4 "Methods for reprogramming somatic cells" and was issued on January 13, 2015. (justia.com)
7 The '536 Patent is entitled "Methods for making somatic cells more susceptible to 8 reprogramming" and was issued on January 27, 2015. (justia.com)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
How well the 58 iPSC lines met quality criteria depended on the origin of the reprogrammed cells (skin vs. blood, male vs. female) and specific reprogramming methods. (neurosciencenews.com)
The different methods included a variety of reprogramming genes, vectors (engineered viruses that deliver genetic material to cells), or the use of plasmids (small DNA molecules that can deliver reprogramming genes). (neurosciencenews.com)

We tested whether the cloned dogs were genetically identical by microsatellite analysis of genomic DNA from the donor Afghan, the cloned dogs and the surrogates (see supplementary information ). (nature.com)
Analysis of eight canine-specific microsatellite loci confirmed that the cloned dogs were genetically identical to their donor dog. (nature.com)
The resulting cells would be genetically identical to the somatic cell donor, thus avoiding any complications from immune system rejection. (wikipedia.org)

The genetic material of the donor egg cell is removed and discarded, leaving it 'deprogrammed. (wikipedia.org)

repens somatic antigen, as described ( 8 ). (cdc.gov)

Cells from the blood samples are reprogrammed into clinical-grade human induced pluripotent stem cells, which can be distributed to recipients for regenerative medicine. (wjgnet.com)

A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patient's particular disease. (wikipedia.org)
Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
A breakthrough in somatic cell nuclear transfer opens the possibility of producing human embryonic stem cells with a patient's own genes. (the-scientist.com)

The term stem cell is generally used to describe cells that are totipotent , pluripotent , or multipotent . (citizendium.org)
These cells are called totipotent stem cells . (orthodoxwiki.org)

As embryonic development proceeds, stem cells lose their pluripotency. (orthodoxwiki.org)

The second being a somatic cell, referring to the cells of the human body. (wikipedia.org)
We validate optical actuation by virally introducing optogenetic drivers in rat and human cardiomyocytes or through the modular use of dedicated light-sensitive somatic 'spark' cells. (nature.com)
Scientists have isolated the first human embryonic stem cell lines specifically tailored to match the nuclear DNA of patients, both males and females of various ages, suffering from disease or spinal cord injury. (scienceblog.com)
These cell lines will enable the study of human disease in cells in the laboratory. (scienceblog.com)
It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
In his original report, Thompson demonstrated that human embryonic stem cells could be coaxed into developing gut-like structures, bone, cartilage and muscle (1). (spiked-online.com)
Current screening of potential new drugs is done using cell lines derived from animals or 'abnormal' human tissue such as tumor cells. (spiked-online.com)
Human nuclear cell transfer is legal in the UK, but is liable to become a criminal act in the USA pending an upcoming vote in the Senate. (spiked-online.com)
The exact process of differentiation is not yet understood and although embryonic stem cells can, in principle, provide for all human tissue, scientists are some way from controlling the process. (spiked-online.com)
It is likely that human stem cells will have similar properties. (spiked-online.com)
Should the use of nuclear transfer ever become widespread there will be an urgent need for human egg donors and it is unlikely that the willing population will be sufficient to meet demand. (spiked-online.com)
Hwang said his team had created a single cell line from 242 human eggs. (koreatimes.co.kr)
Religious groups believe that the raw material from which stem cells are sourced are themselves forms of human life, and by creating little chunks of humans in Petri dishes, scientists are, critics believe, playing God. (koreatimes.co.kr)
Researchers are investigating several approaches, such as enzymatic treatments of RBCs, the generation of RBCs from human induced pluripotent stem cells, and the development of artificial oxygen carriers, all with the goal of advancing universal blood. (cap.org)
The first pluripotent human embryonic stem cells (hESCs) have been generated from somatic cell nuclear transfer, according to a study published today (October 5) in Nature . (the-scientist.com)
A) shows human embryonic stem cells. (citizendium.org)
After sixteen weeks, the injured mice who received human stem cell injections experienced a significant improvement in the motor functions that had been impaired by their injuries. (citizendium.org)
Properly controlled, adequately sized studies have yet to demonstrate that human embryonic stem cells have medical value in humans. (citizendium.org)
On appeal, the Roslin Institute argued that unlike the donor sheep used to create Dolly, clones like Dolly are eligible for protection because they are 'the product of human ingenuity' and 'not nature's handiwork, but [their] own. (patentdocs.org)
Shown here, an 8-cell human embryo used for in vitro fertilization. (alleninstitute.org)
They represent a permanent forensic map of the whole cell lineage of the whole human body. (alleninstitute.org)
A controversial scheme to extend the practise of 'egg sharing' has been approved by the UK's Human Fertilisation and Embryology Authority (HFEA) to provide greater numbers of eggs for embryonic stem (ES) cell research. (progress.org.uk)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
We know that over time, somatic mosaicism can lead to diseases like cancer, but we don't know how much somatic mosaicism there is in our personal genomes or how much it influences human biology. (nih.gov)
The goal of the SMaHT Network is to determine how somatic mosaicism impacts human biology and health. (nih.gov)
The human body is made of billions and billions of cells, which have specific shapes, particular structures, and different functions. (orthodoxwiki.org)
Stem cells are naturally occurring in the human body (and other living organisms) at all levels of development. (orthodoxwiki.org)
In the fetus, stem cells in developing tissue give rise to the multiple specialized cell types that make up the human body. (orthodoxwiki.org)
Researchers also compared the molecular and functional characteristics of iPSCs to human embryonic stem cells, which are used sparingly as a gold standard for benchmarking quality. (neurosciencenews.com)
… "embryo" means a human organism during the first 56 days of its development following fertilization or creation, excluding any time during which its development has been suspended, and includes any cell derived from such an organism that is used for the purpose of creating a human being. (hinxtongroup.org)
6.Future of Stem Cell Research A)In conclusion, stem cell research holds the answers too many of the questions that we are looking for regarding the human body. (web.app)
Application of Human Pluripotent Stem Cells in T Cell Based Cancer. (web.app)
The question of anonymity reveals that the gametes, by transmitting the heredity, represents more than blood or skin cells…The confusion of the statuses of germinal cells and simple somatic cells to copy exactly the use of the first one on the second one (donations, banks, etc.) and the organised dissociation of the filiation (biological and social), lead us to complex and delicate human situations. (genethique.org)
They are already created in the human body and can only replenish the same type of cells as it started out with. (freeessays.education)
The funding received from the Swedish Research Council will go towards his research, 'mapping human dopamine neuron diversity at single-cell resolution for improved stem cell therapy in Parkinson's disease. (lu.se)

The aim of carrying out this procedure is to obtain pluripotent cells from a cloned embryo. (wikipedia.org)
Currently, the procedure for isolating non-reproductive cells for the nuclear transfer method involves animal enzymes and serum. (scienceblog.com)
Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)

The work also moves scientists one step closer to the goal of transplanting healthy cells into humans to replace cells damaged by diseases such as Parkinson's and diabetes. (scienceblog.com)
In humans, somatic transfer has been less fruitful-the egg cell quits dividing and often dies after nuclear transfer. (the-scientist.com)
Early progenitor cells, which are designated A spermatogonia in the mouse and A- dark spermatogonia in humans, are defined as "undifferentiated. (jci.org)
From this point forward, the steps and cell types of spermatogenesis are conserved between mice and humans. (jci.org)
Humans are accidental hosts with patent from clinically healthy blood donors living in a D . infections being extremely rare. (cdc.gov)

Instead of removing the egg genome prior to nuclear transfer, he and his colleagues added the somatic cell nucleus directly to the intact egg. (the-scientist.com)
For the first time researchers can now compare iPSC differentiation to the same process an egg goes through after the transfer of a somatic cell genome. (the-scientist.com)

it is shown in Fig. 1a with the male Afghan fibroblast donor. (nature.com)

Using single-cell RNA sequencing (RNAseq), we studied the development of ProSG, their SG descendants and testicular somatic cells during the perinatal period in mice. (biologists.com)
Undifferentiated spermatogonia divide mitotically to both repopulate the testicular stem cell population and provide progenitor cells that undergo spermatogenesis. (jci.org)

The process of somatic cell nuclear transfer involves two different cells. (wikipedia.org)
Somatic cell nuclear transfer typically involves the transfer of genomic information from a somatic cell into an unfertilized egg cell whose nucleus has been removed. (the-scientist.com)
[ 64 ] Screening for PNH involves detection of deficiency of glycosylphosphatidyl-inositol (GPI) anchored proteins, such as CD14, CD16 and CD24, as well as fluorescent aerolysin (FLAER) for white blood cells, and CD55 and CD59 for red cell analysis, on flowcytometry. (medscape.com)

From the 31 nuclear-transfer blastocysts, the scientists derived 11 stem cell lines. (scienceblog.com)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
It might be expected that the richest nation on Earth would encourage its top scientists to pursue this work with vigor rather than limiting funding opportunities, creating legal barriers and fencing off any newly developed cell lines. (spiked-online.com)
This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
Part of the Progenitor Cell Biology Consortium, the scientists are working to make sure this growing area of medical research is grounded in safe and sound science. (neurosciencenews.com)

Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
The Court disagreed, stating that 'Dolly herself is an exact genetic replica of another sheep and does not possess 'markedly different characteristics from any [farm animals] found in nature,'' and thus, 'Dolly's genetic identity to her donor parent renders her unpatentable. (patentdocs.org)

ASD brains show an excess of somatic mutations in neural enhancer sequences compared with controls, suggesting that mosaic enhancer mutations may contribute to ASD risk. (nih.gov)
To sketch that detailed map, the researchers captured the complete DNA sequences from thousands of different cells from more than 70 people who had died and donated their bodies to science. (alleninstitute.org)

As a result, an integrative (both cell-level and multicellular) view is essential, and current regulations need to be revisited (see Supplementary Fig. 1 overviewing the Comprehensive in Vitro Pro-arrhythmia Assay 5 Concept). (nature.com)
Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
The stem cells in storage at Hwang's laboratories at the university were all derived from the fertility clinic MizMedi's blastocysts, which were generated by in vitro fertilization . (blogspot.com)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)

Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
The transplant resulted in sustained Comment donor-recipient chimerism and correction of the Comment immunodeficiency. (lu.se)

Resilient blood centers implemented operational changes and intensified donor recruitment efforts to stabilize the situation. (cap.org)
The Data Analysis Centers will ensure that the somatic variant catalog is accessible, high-quality, and interoperable. (nih.gov)

However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
Typically, these technologies start by engineering a single progenitor cell with artificial transcribed recording sites that accumulate stable insertions or deletions ("indels") as a result of repair of Cas9 double-stranded breaks. (biorxiv.org)

Other improvements over the last paper include the reduced use of animal products in laboratory procedures and better evidence that the cell lines matched the patients' cells and did not have a parthenogenetic origin, where unfertilized eggs can divide on their own. (scienceblog.com)
Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
It is hoped that one donor per week will be recruited and that each new recruit will donate six to ten eggs for the research. (progress.org.uk)
Peter Braude, Professor of Obstetrics and Gynaecology at Kings College London, who has also been granted HFEA licences for embryonic stem cell research in the past commented, 'This is a difficult situation because there is a strong need for eggs for research. (progress.org.uk)

MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. (web.app)

However, donor organs are in absolute shortage, and sadly, most patients die while waiting for a donor organ. (frontiersin.org)
Presently, in the United States, another person is added to an organ transplant list every 10 min, 17 people die each day while waiting for donor organs, and approximately 105,800 patients are waitlisted for an organ transplant according to the health resources and services administration (HRSA). (frontiersin.org)

8 Specifically, Plaintiffs allege that Defendants, individually and acting in concert, make, 9 use, sell, offer for sale, and/or import induced pluripotent stem cells ("iPSCs") that infringe 10 one or more claims of the asserted patents.1 (Id. (justia.com)
212 ("iPSCs used by Defendants to make at least the iPSC-derived 13 natural kill (NK) cell platforms are made by a process that comprises at least each step of 14 claim 1 of the '856 Patent. (justia.com)
Plaintiffs allege that Fate is the 21 22 23 24 25 26 27 28 1 Induced pluripotent stem cells ("iPSCs") "are pluripotent stem cells generated from somatic cells by reprogramming. (justia.com)
This microscopic image shows a cross section from a teratoma, generated in the lab by authors of a June 9 study in Stem Cell Reports that tested the quality of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
In a study published June 9 by the journal Stem Cell Reports , and funded by the National Heart, Lung, and Blood Institute, the multi-institutional research team reports on the comprehensive characterization of a large set of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
Although the technology to produce safe and effective iPSCs exists, study authors report they encountered an unexpected number of wobbly production processes for the cells. (neurosciencenews.com)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. (web.app)
Phylogenetic trees are routinely constructed to describe the developmental relationships within sets of extant taxa such as different organisms, proteins, or single cells. (biorxiv.org)

That's in contrast to a germline mutation, a mutation in a sperm or egg cell that would pass onto the next generation and appear in every cell alike. (alleninstitute.org)
The inactivating mutation resulted in the loss of LHR expression on the cell surface and no cAMP signaling. (bvsalud.org)
Finally, a mis-tuned "Cas9 editing rate" - the rate at which Cas9 induces heritable mutations used for lineage tracing - can lead to scenarios where there is a lack of mutation information sufficient for discerning relationships between cells. (biorxiv.org)
In 2005, researchers discovered a mutation in the Janus Tyrosine Kinase 2 gene (JAK2 (V617F)), which plays a pivotal role in the regulation of blood cell production (Levine et al. (cdc.gov)
the JAK2 mutation, the presence of the mutation has become an important diagnostic criterion for identifying patients with PV and for reducing the potential for misdiagnosis of persons with elevated red blood cell counts. (cdc.gov)

Anatomy62

Organisms22

Diseases10

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Psychiatry and Psychology1

Phenomena and Processes89

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science3

Named Groups3

Health Care5

SCNT29

Oocyte14

Nuclei10

Oocytes5

Mammalian2

Termed somatic mutations1

Adult34

Transplantation10

Tissues13

Germ11

Differentiate to all cell types1

Fibroblasts2

Genetically identical to the donor1

Neurons9

Pluripotent state3

Therapeutic11

Fetal3

Mutations-mutations1

Produce embryonic stem1

Diploid1

Autologous4

Embryos12

Healthy donors3

Therapies9

Clone8

Informed consent of the donor1

Blastocyst8

Cell's nucleus2

Cloning9

Single-nucleotide2

Methods8

Identical3

Genetic material1

Antigen1

Regenerative Medicine1

Patient's3

Totipotent2

Embryonic development1

Human35

Procedure3

Humans5

Genome2

Afghan1

Testicular2

Involves3

Scientists6

Dolly2

Sequences2

Vitro4

Recipient2

Centers2

Progenitor2

Eggs4

Biology2

Organs2

IPSCs8

Developmental2

Mutation5