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  • SCNT
  • Here, we identify histone H3 lysine 9 trimethylation (H3K9me3) of donor cell genome as a major barrier for efficient reprogramming by SCNT. (nih.gov)
  • RRRs are enriched for H3K9me3 in donor somatic cells and its removal by ectopically expressed H3K9me3 demethylase Kdm4d not only reactivates the majority of RRRs, but also greatly improves SCNT efficiency. (nih.gov)
  • animal
  • As pre-implantation and pre-natal chromosomal and genetic screening was not performed in any of the aforementioned animal cloning experiments, a small but significant proportion of the resulting offspring exhibited developmental abnormalities and/or perinatal death. (zavos.org)
  • human
  • Dr. Panos Michael Zavos, founder, Andrology Institute of America testifies on Capitol Hill Wednesday, March 28, 2001 before the House subcommittee on Oversight and Investigations hearing on issues raised by human cloning research. (zavos.org)
  • It is because of our total dedication and belief in those principles that I have decided along with Prof. Antinori to undertake the great effort and to offer our infertility patients that have exhausted all options available to them, to bear a biological child of their own through the option of human therapeutic cloning. (zavos.org)
  • vitro
  • I have also received extensive training in the areas of gamete physiology, manipulation, cell culture and in-vitro gamete manipulation. (zavos.org)
  • pigs
  • The authors conclude that although in this early phase of research no direct evidence can be provided about the practical use of transgenic pigs produced by somatic cell nuclear transfer as organ donors or disease models, the future chances even in medium term are good, and at least proportional with the efforts and sums that are invested into this research area worldwide. (publish.csiro.au)
  • world's
  • On November 2015, a Chinese biotech company Boyalife Group announced that it will partner with Hwang's laboratory, Sooam Biotech, to open the world's largest animal cloning factory in Tianjin as early as 2016. (wikipedia.org)
  • fetus
  • The scientist can then either remove the stem cells from this blastocyst, or place it into a uterus where it has the potential to develop into a fetus. (eurekalert.org)
  • For a viable fetus to develop, the egg needs to reprogram the genome of the skin cell, shutting off genes specific for skin tissue and turning on genes needed for embryonic development, genes that are normally dormant in tissue-specific cells. (eurekalert.org)
  • macaque
  • The third macaque monkey cloned from somatic cells will soon be born in China, Vice Minister of Science and Technology Huang Wei revealed on Sunday. (china-underground.com)
  • They extracted nuclei from the fibroblasts of an aborted fetal monkey (a crab-eating macaque or Macaca fascicularis) and inserted them into egg cells (ova) that had had their own nuclei removed. (wikipedia.org)
  • adult cell
  • This demonstrated for the first time that the DNA in a somatic cell (specifically, a breast cell) still contained all the information needed to direct the formation of an entire organism, and that this information could be made accessible again in the adult cell. (bioedonline.org)
  • 1997
  • In 1997, Ritchie and Campbell in collaboration with PPL (Pharmaceutical Proteins Limited) created another sheep named "Polly", created from genetically altered skin cells containing a human gene. (wikipedia.org)
  • Scientists
  • Ltd. is live, dead & ancestral human somatic cell preservation (To Clone Human in Future) and innovative health care product company, driven by highly experienced scientists having registered office in India. (endorphins-healthcare.com)
  • Scientists generally agree that all cloned animals are biologically flawed. (eurekalert.org)
  • Analyzing the complete gene-expression profiles of both cloned and fertilization-derived stem cells in mice, scientists at Whitehead Institute for Biomedical Research now have concluded that the two are, in fact, indistinguishable. (eurekalert.org)
  • This is one reason why scientists generally believe that attempting to clone a human being is morally reprehensible. (eurekalert.org)
  • Although these animals generated entirely from cloned stem cells appear to be fine, many scientists don't accept this result as definitive. (eurekalert.org)
  • The scientists also attempted to clone macaques using nuclei from adult donors, which is much more difficult. (wikipedia.org)
  • blastocyst
  • But this entire process is almost never perfect, and nearly all cells in a cloned blastocyst retain some memory of their original source. (eurekalert.org)
  • Studies have demonstrated that a small number of stem cells in the blastocyst appear to be spared this faulty reprogramming. (eurekalert.org)
  • When stem cells from a cloned blastocyst are removed and placed into a dish, most die. (eurekalert.org)
  • The hybrid cell is then stimulated to divide by an electric shock, and when it develops into a blastocyst it is implanted in a surrogate mother. (wikipedia.org)
  • Theriogenology
  • He was a professor of theriogenology and biotechnology at Seoul National University (dismissed on March 20, 2006) who became infamous for fabricating a series of experiments, which appeared in high-profile journals, in the field of stem cell research. (wikipedia.org)
  • humans
  • Our overriding goal is to safely preserve the live humans, critically ill humans, loved ones who passed away & ancestral somatic cells to produce human clone through reproductive cloning technology in near future when reproductive human cloning become absolutely safe through advancement of technology and ethics and government law permits. (endorphins-healthcare.com)
  • Methods to reprogram normal body cells into pluripotent stem cells were developed in humans in 2007. (bootstrike.com)
  • Researchers
  • For the current experiment, published in Cell Stem Cell , the researchers combined the two approaches, by using cells where the Xist gene was knocked out, and then injecting Kdm4d messenger RNA. (medicalnewser.com)
  • Researchers have tried to test the integrity of these surviving stem cells by transplanting them into fertilized blastocysts and then observing the overall health of the resulting animal. (eurekalert.org)
  • This crucial step allowed the researchers to overcome the main obstacle that had prevented the successful cloning of primates until now. (wikipedia.org)
  • Artificial
  • She is the first "cloned" primate by artificial twinning, and was created by a team led by Professor Gerald Schatten of the Oregon National Primate Research Center. (wikipedia.org)
  • 1999
  • Hwang's next claim came only two months later in April 1999, when he announced the cloning of a Korean cow, Jin-i, also without providing any scientifically verifiable data. (wikipedia.org)
  • Wilmut
  • citation needed] Working at the Roslin Institute since 1991, Campbell became involved with the cloning efforts led by Ian Wilmut. (wikipedia.org)
  • stem cell res
  • On May 12, 2006, Hwang was charged with embezzlement and bioethics law violations after it emerged much of his stem cell research had been faked. (wikipedia.org)
  • The Korea Times reported on June 10, 2007, that Seoul National University fired him, and the South Korean government canceled his financial support and barred him from engaging in stem cell research. (wikipedia.org)
  • In February 2011, Hwang visited Libya as part of a $133 million project in the North African country to build a stem cell research center and transfer relevant technology. (wikipedia.org)
  • Expression
  • 2010. Impeding Xist Expression from the Active X Chromosome Improves Mouse Somatic Cell Nuclear Transfer. (koreascience.or.kr)
  • Tobias Brambrink, a postdoctoral researcher in the Jaenisch lab, tried a different approach, comparing gene expression in cloned and fertilization-derived stem cells. (eurekalert.org)
  • Conversely, UCK2 has been detected mostly in cancerous cells and healthy placental tissue.The selective expression in target tissues has resulted in the identification of UCK2 as a target in anti-cancer therapies. (wikipedia.org)
  • sheep
  • After a certain number of cell divisions, the ball of cells was implanted into a pseudo-pregnant sheep. (bioedonline.org)
  • Some in the press speculated that a contributing factor to Dolly's death was that she could have been born with a genetic age of six years, the same age as the sheep from which she was cloned. (wikipedia.org)
  • antibodies
  • Biologically, as a result of junctional recombination, P/N nucleotide editing and somatic hypermutation (for BCR/antibodies) [ 14 ], the protein sequence space of CDR3s is immense and renders the finding of significant overlap between repertoires highly unlikely. (beds.ac.uk)
  • mice
  • The experimental elimination of senescent cells from transgenic progeroid mice and non-progeroid, naturally-aged mice led to greater resistance against aging-associated diseases. (wikipedia.org)
  • antibody
  • However, the cited gene changes can go awry in plasma cells by, for example, placing a gene that ordinarily controls cell growth adjacent to the normally highly active antibody gene promotor thereby creating a cancer-causing oncogene or, more commonly, by forming extra chromosomes (see trisomy) or chromosomes that have deleted or repetitive sections, any of which such changes may promote malignancy in more complex and less well understood ways. (wikipedia.org)
  • cellular
  • Cellular senescence is the phenomenon by which normal diploid cells cease to divide. (wikipedia.org)
  • As such, cellular senescence represents a change in "cell state" rather than a cell becoming "aged" as the name confusingly suggests. (wikipedia.org)
  • Consistent with this, telomerase-immortalised cells continued to age (according to the epigenetic clock) without having been treated with any senescence inducers or DNA-damaging agents, re-affirming the independence of the process of epigenetic ageing from telomeres, cellular senescence, and the DNA damage response pathway. (wikipedia.org)
  • known
  • Interferon type I: All type I IFNs bind to a specific cell surface receptor complex known as the IFN-α/β receptor (IFNAR) that consists of IFNAR1 and IFNAR2 chains. (wikipedia.org)
  • types
  • Expression of type I and III IFNs can be induced in virtually all cell types upon recognition of viral components, especially nucleic acids, by cytoplasmic and endosomal receptors, whereas type II interferon is induced by cytokines such as IL-12, and its expression is restricted to immune cells such as T cells and NK cells. (wikipedia.org)
  • activity
  • Coat colour in mammals is determined by the presence, distribution and biochemical activity of the melanocytes, which are specialized cells where eumelanins (black/brown pigments) and pheomelanins (yellow/red pigments) are synthesized. (biomedcentral.com)
  • They also limit viral spread by increasing p53 activity, which kills virus-infected cells by promoting apoptosis. (wikipedia.org)
  • Although senescent cells can no longer replicate, they remain metabolically active and commonly adopt an immunogenic phenotype consisting of a pro-inflammatory secretome, the up-regulation of immune ligands, a pro-survival response, promiscuous gene expression (pGE) and stain positive for senescence-associated β-galactosidase activity. (wikipedia.org)
  • entire
  • Here, we introduce a bioinformatics repertoire-profiling framework that possesses the advantage of capturing the diversity and distribution of entire immune repertoires, as opposed to singular public clones. (beds.ac.uk)
  • Another
  • Another application of SCNT stem cell research is using the patient specific stem cell lines to generate tissues or even organs for transplant into the specific patient. (wikipedia.org)
  • specific
  • These cells genetically matched the donor organism from which they came.This gives them the ability to create patient specific pluripotent cells, which could then be used in therapies or disease research. (wikipedia.org)
  • The disease specific stem cell lines could then be studied in order to better understand the condition. (wikipedia.org)
  • forms
  • The overproduced Ig's, termed myeloma proteins, commonly circulate in blood, may accumulate in urine, and are the hallmarks of plasma cell dyscrasias including their most malignant forms viz. (wikipedia.org)
  • ability
  • Interferons are named for their ability to "interfere" with viral replication by protecting cells from virus infections. (wikipedia.org)