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SolventsChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, GasChromatography, Ion ExchangeChromatography, Thin LayerChromatography, LiquidMolecular WeightChromatography, DEAE-CelluloseElectrophoresis, Polyacrylamide GelKineticsChromatography, AgaroseMass SpectrometryMolecular Sequence DataGas Chromatography-Mass SpectrometryAmino Acid SequenceAcetonitrilesWaterHexanesHydrogen-Ion ConcentrationChromatography, Reverse-PhaseAmino AcidsSpectrophotometry, UltravioletChromatography, PaperMethanolCountercurrent DistributionMagnetic Resonance SpectroscopyChemistryTemperatureTolueneSubstrate SpecificityPaintProtein ConformationSolubilityChemical PhenomenaAcetoneModels, MolecularCattleSpectrometry, Mass, Electrospray IonizationDimethylformamideChemical FractionationMolecular StructureSepharoseChloroformTandem Mass SpectrometryDrug StabilityEscherichia coliProtein BindingMethylene ChlorideOrganic ChemicalsThermodynamicsCarbohydratesBinding SitesModels, ChemicalMethodsSolutionsHydrolysisTrichloroethanesPeptidesPeptide FragmentsProteinsIndicators and ReagentsIsoelectric FocusingChemistry, PhysicalCrystallizationRecombinant ProteinsMacromolecular SubstancesPhysicochemical PhenomenaCarbohydrate SequenceRabbitsTrypsinStereoisomerismXylenesChromatography, Micellar Electrokinetic CapillaryPlant ExtractsVolatilizationAlcoholsIonic LiquidsLiverSpectrometry, FluorescenceCircular DichroismIsoelectric PointHydrogen BondingOligosaccharidesCrystallography, X-RayOccupational ExposureDimethyl SulfoxideCalibrationCatalysisChromatography, Supercritical FluidReproducibility of ResultsEnzyme StabilityChemistry Techniques, AnalyticalBacterial ProteinsMicrochemistryReference StandardsEthylene GlycolsSolid Phase Extraction
HPLCThin layer chromatoAchieved by Flash chromatographyMass SpectrometrySilica gelExtractionPaper ChromatographyOrganic solventNormal phase solventsSupercritical carbonAqueousAcetonitrileVolatileChromatographicPolarityNonpolarCompoundsEthanolFlammable solventsSeparationsAnalyticalRecrystallizationStationaryPolarAcetoneImpuritiesDeterminationDissolveMiscibleDerivatizationAerosolEthyl acetateGradientsGradientSample PreparationPharmaceuticalAdsorptionChemicalLaboratoryHydrocarbonResinTemperatureToxicReactionDetectionRetentionPesticidesResidueColumnTetrahydrofuranAlcoholsMobile phasePolymer
HPLC16
Thin layer chromato5
Achieved by Flash chromatography1
Mass Spectrometry4
  • The procedure involves enzymatic hydrolysis of urine, extraction, derivatization, and column cleanup, followed by analysis of the concentrate using capillary gas chromatography combined with tandem mass spectrometry (GC-MS/MS). This procedure uses isotope dilution with 13 C-labeled internal standards for all analytes. (cdc.gov)
  • The method used for qualitative confirmation of identified analyte is gas chromatography/mass spectrometry. (epa.gov)
  • The derivatized carbonyls are extracted from the pads and subsequently quantified by ultra-high-pressure liquid chromatography coupled with tandem mass spectrometry. (cdc.gov)
  • We developed and validated the first quantitative liquid chromatography-tandem mass spectrometry assay for measuring urinary concentrations of pre-PhMA. (cdc.gov)
Silica gel6
  • True or False: A sample of aspirin has an $R_f$ value of 0.20 when measured on a silica gel TLC plate with a certain solvent mixture. (stackexchange.com)
  • IE solvent-resistant chiral column, the amylose-tris (3,5-dichlorophenylcarbamate) covalently bonded to the surface of the silica gel. (thelabstore.co.uk)
  • As a modifier, you can use all solvents that can be used for chromatographic columns such as methanol, ethanol, 2-propanol, acetonitrile, THF, halogen solvents and other silica gel-based chromatography columns. (thelabstore.co.uk)
  • After the respective times of reaction described in Table 1 , crude products were isolated, filtered over column chromatography of silica gel, and then analysed by 1 H NMR. (hindawi.com)
  • Separation of dried liquid samples with a liquid solvent and a glass plate covered with a thin layer of silica gel. (majortests.com)
  • If you poured your mixture directly on top of you silica gel, and used a solvent not miscible with water such as hexanes, you would end up with phase separations inside your column. (chemicalforums.com)
Extraction14
  • Conventional extraction techniques are usually based on highly pollutant and/or flammable organic solvents. (frontiersin.org)
  • Natural deep eutectic solvents (NADES) are a green and nontoxic attractive alternative to hydroalcoholic extraction. (frontiersin.org)
  • This study aimed to investigate the application of two NADES, namely, betaine:triethylene glycol (Bet : TEG) and choline chloride:1,2-propanediol (Chol : Prop), as sustainable green solvents for the extraction of polyphenols from spent coffee ground (SCG), a by-product of coffee processing. (frontiersin.org)
  • Natural deep eutectic solvents (NADES) emerge as an alternative ecofriendly extraction method. (frontiersin.org)
  • Solvent extraction and separation tech. (slideshare.net)
  • Similar a Solvent extraction and separation tech. (slideshare.net)
  • Such mixed solutions are also useful in separation science, as extraction solvents and in liquid chromatography. (scirp.org)
  • The specific microfluidic flow, TRDF, was applied to capillary liquid chromatography [8] and micro-extraction [9]. (scirp.org)
  • Determination of chlorination disinfection by products and chlorinated solvents in drinking water by liquid-liquid extraction and gas chromatography with electron-capture detection : [test] method 551.1. (epa.gov)
  • solvent gradient chromatography, chromatotron, selective solvent extraction and medium pressure liquid chromatography (MPLC) were employed. (who.int)
  • Selective solvent extraction using ethyl acetate from a methanol extract is a simple method of isolating FD from flabelliferin profiles containing triglycosides and tetraglycosides. (who.int)
  • A technique based on direct MPLC (without mentanol extraction, petroleum ether cleaning, acetone extraction and dry cellulose chromatography was worked out to separate not only the flabelliferins in their pure state but also the carotenoids and the free sugars in PFP. (who.int)
  • Of special interest is the use of supercritical carbon dioxide and pressurised hot liquid water as solvents in extraction and chromatography. (lu.se)
  • Charlotta Turner researches the challenge of how to replace organic solvents with supercritical carbon dioxide and pressurized hot water, in separation processes such as extraction, chromatography, and crystallization. (lu.se)
Paper Chromatography1
Organic solvent13
  • In HILIC mode, water acts as the strongest solvent to effectively elute analytes off the column, and the organic solvent acts as the weak solvent. (thermofisher.com)
  • HILIC mode also eliminates the need for evaporation and reconstitution of samples dissolved in a highly organic solvent, allowing sample analysis throughput to be greatly increased. (thermofisher.com)
  • The mobile phase for a HILIC separation should consist of a low percentage of water and a high percentage of an organic solvent such as acetonitrile. (thermofisher.com)
  • When running HILIC separations, it is important to use an organic solvent without alcohol functional groups because alcohol functional groups will disrupt the water layer required around the stationary phase, causing inconsistent results. (thermofisher.com)
  • Acetonitrile is the most commonly used organic solvent without an alcohol functional group, making it ideal for use in HILIC. (thermofisher.com)
  • Urine samples are prepared by extracting with an organic solvent and concentration of the solute. (cdc.gov)
  • Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. (scirp.org)
  • Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. (scirp.org)
  • The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. (scirp.org)
  • Chemiluminescence in the presence of twenty types of α -amino acid was similarly examined by using the ternary organic solvent-rich solution. (scirp.org)
  • The mixing behavior of ternary water-hydrophilic/hydrophobic organic solvent solutions was investigated using a Y-type microchannel. (scirp.org)
  • Denaturation was examined in a ternary mixed solution of water-hydrophilic/hydrophobic organic solvent using λ-DNA and a plasmid as models. (scirp.org)
  • The novel idea of using the ternary mixed solution first enabled the interaction of the hydrophobic organic solvent molecule with the double helical structure of DNA [11]. (scirp.org)
Normal phase solvents1
Supercritical carbon1
  • By exploiting properties of supercritical carbon dioxide (sc-CO2), such as solvating power and enhanced diffusivity, organosilanes are reacted with surface silanol groups, for a clean, solvent-free preparation of highly efficient silica bonded phases for liquid chromatography (LC), such as octadecylsilica, fluorinated octylsilica and pentafluorophenylpropyl phases (1). (labmate-online.com)
Aqueous1
Acetonitrile1
  • In our previous study, we examined luminal chemiluminescence (CL) behavior in a ternary mixed solvent solution of water-acetonitrile-ethyl acetate, and the mixed solution was found to enhance luminol CL in time and intensity under certain conditions [12]. (scirp.org)
Volatile4
Chromatographic1
  • Modern flash chromatographic system consists of pre-packed plastic cartridges wherein solvent is pumped through the cartridges to achieve separation. (drinksavvyinc.com)
Polarity5
  • If the polarity of the solvent increases, will the Rf value increase or decrease? (stackexchange.com)
  • If the polarity of the solvent is increased, the $R_f$ value of the aspirin will become larger. (stackexchange.com)
  • So as to Pick the right kind of solvent, get the polarity of the sample match it with the polarity of the sample. (markmerlis.com)
  • If the sample has greater Polarity, then the solvent has to have the opposite pole. (markmerlis.com)
  • Normal and Reversed Phase Chromatography What is the relationship between polarity of the mobile phase vs. elution time and resolution in normal phase and reversed phase chromatography? (majortests.com)
Nonpolar3
Compounds3
  • This is an air pressure driven technique comprising of medium and short column chromatography, optimised for rapid separation of organic compounds. (drinksavvyinc.com)
  • Modern Flash chromatography with disposable cartridges and advanced detection techniques is applicable to a wide range of compounds. (drinksavvyinc.com)
  • During this day, students from the Grade 7 class extracted compounds from various plant and flower samples, which were then loaded on a silica based chromatography column for characterization. (ubc.ca)
Ethanol4
Flammable solvents4
  • Certain areas in the lab or around storage facilities and cupboards are EX-classified due to possible closeness to flammable solvents and gases. (lu.se)
  • The facilities in lab, arranged for handling flammable solvents and gases, such as fume hoods and storage cupboards, has been constructed and adjusted to fulfil the requirements by the ruling authorities. (lu.se)
  • Work with flammable solvents or gases must only be carried out in fume hoods or other workplaces with forced ventilation. (lu.se)
  • Use only tap water cooling instead of pump circulated cooling during reflux boiling of flammable solvents. (lu.se)
Separations2
  • Gradient grade LiChrosolv® solvents enable you to minimize the gradient effect of the solvent involved - for example in enantiomeric separations on chiral phases - and are suitable for UPLC and UHPLC. (emdmillipore.com)
  • Achieve better gas chromatography separations with an appropriate column. (sigmaaldrich.com)
Analytical2
  • Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results. (sigmaaldrich.com)
  • All the other solvents and chemicals were of analytical grade and were obtained from S.D. Fine-Chem. (ijpsonline.com)
Recrystallization5
Stationary5
  • Like other forms of chromatography, GC involves a stationary phase and a mobile phase. (sigmaaldrich.com)
  • In GC, the mobile phase is an inert gas, usually helium or nitrogen, and the stationary phase is either a solid adsorbent, termed gas-solid chromatography (GSC), or a liquid adsorbed onto an inert support, termed gas-liquid chromatography (GLC, or just GC). (sigmaaldrich.com)
  • A novel method for the preparation of stationary phases has been developed, using a green chemistry approach that avoids the use of problematic organic solvents. (labmate-online.com)
  • Chromatography is the separation of mixtures into their components as a result of differential distribution of the solutes in order for identification, analyses, purify and/or quantity of the mixture for a mobile medium and for a stationary absorbing medium. (majortests.com)
  • I understand that the purpose of the column chromatography is to separate away any unreacted benzoin (the hydroxyl group would be attracted to the stationary silica layer). (chemicalforums.com)
Polar2
  • The only appropriate one-component solvent systems (listed from the least polar to the most polar): Hydrocarbons: pentane, petroleum ether, hexanes. (drinksavvyinc.com)
  • Firstly, the retention diagrams of some polar and non-polar solvents on PVBC-Dibutyl amine were obtained over a temperature range from 135 to 160° C. Then, the thermodynamic parameters, including Flory-Huggins interaction parameter, weight fraction activity coefficient, partial molar heat of sorption, and partial molar heat of mixing were determined via interactions between the polymer and selected solvents at infinite dilution. (yildiz.edu.tr)
Acetone2
Impurities2
  • When choosing your solvents, you also have to consider the UV absorbance of the solvents as this can vary from one type to another, depending upon the nature and concentration of solvent impurities. (drinksavvyinc.com)
  • Chromatography, even imperfect chromatography, can get you in the ballpark, and then even impurities very similar in nature to the product can be removed by crystallization. (chemicalforums.com)
Determination1
  • The concentrate is analyzed using liquid chromatography combined with tandem mass spectrometric (LC/MS/MS) determination. (cdc.gov)
Dissolve2
Miscible1
  • Unrestricted use of solvents, a wide range of dissolution conditions, the use of any miscible solvent as a mobile phase, dissolved samples, chiral column regeneration. (thelabstore.co.uk)
Derivatization1
  • The traditional method of measuring carbonyls in smoke involves solvent-filled impinger trapping and derivatization. (cdc.gov)
Aerosol2
  • DCM is used primarily as a solvent in paint removers, degreasers, aerosol products and the manufacture of foam polymers. (who.int)
  • Occupation exposure occurs through its use as a degreaser, paint remover, aerosol propellant, blowing agent for polymer foam, and as a solvent in the textile industry, photographic film production (cellulose triacetate). (who.int)
Ethyl acetate1
  • The results reveal that the alkanes are poor solvents for the polymer whereas n-butyl acetate, i-butyl acetate, ethyl acetate, n-propylbenzene, i-propylbenzene, and ethylbenzene are moderately solvents in the studied temperature range. (yildiz.edu.tr)
Gradients2
  • During separation, there is an increase of the compactness of components but by using solvent gradients, breakage of this chemical will be reached. (markmerlis.com)
  • Remember that it is not always possible in TLC but should be possible in flash chromatography where solvent gradients can be used. (drinksavvyinc.com)
Gradient1
  • To note, these solvents have to reached its double strength every time it is used in the gradient. (markmerlis.com)
Sample Preparation1
Pharmaceutical1
  • Examples of when chromatography is used are in pharmaceutical companies in order to find out the amount of chemicals used in each new product, hospitals for detection of sugar/blood levels and law enforcement for comparisons of samples found at a crime scene. (majortests.com)
Adsorption4
  • Two pairs of solvents can be combined to result a fantastic procedure of discovery but these solvents should have a gap of adsorption of energy at hundred percent. (markmerlis.com)
  • The effects of dye-adsorption solvent on the performances of dye-sensitized solar cells based on phenothiazine dyes were investigated in this study. (hindawi.com)
  • Cell performance using EtOH as a dye-adsorption solvent showed relatively higher performance than that using THF. (hindawi.com)
  • Electrochemical and photochemical tests of phenothiazine dyes in solution and adsorbed on the TiO 2 surface showed less dye loading and coverage on the TiO 2 surface during adsorption in the case of THF, which decreased the solar cell performance of the DSSC using THF as adsorption solvent compared with using EtOH as adsorption solvent. (hindawi.com)
Chemical3
  • These solvents are manufactured and distributed by reputable chemical businesses across the nation. (markmerlis.com)
  • In June ​, a toxic substance leak at a solvent manufacturing facility in Pavia, Italy run by specialty chemical firm Bitolea caused 39 workers to be hospitalised after reporting burns to their respiratory tracts and eyes. (outsourcing-pharma.com)
  • IBA can be used as a solvent for coating and as a chemical intermediates in organic synthesis. (dcc.com.tw)
Laboratory2
  • Further attractive products to complete your chromatography laboratory can be found on our Chromatography page! (carlroth.com)
  • Transportation of volumes more than 1 L of highly flammable solvent/gases in areas outside the laboratory must be given the highest attention regarding safety. (lu.se)
Hydrocarbon1
  • 1992. Standard speciation for mineral spirits (petroleum spirits) (hydrocarbon dry cleaning solvent). (cdc.gov)
Resin1
Temperature1
  • Negligible loss under vacuum (2 mm Hg) at 20 C. Stability: The technical solid decomposes slowly at room temperature but is stable in organic solvents (e.g. cyclohexanone, methyl ethyl ketone). (inchem.org)
Toxic2
  • Toxic effects of solvents and vapors. (cdc.gov)
  • Methanol is a toxic alcohol that is used industrially as a solvent, pesticide, and alternative fuel source. (cdc.gov)
Reaction1
  • The mixed solvent system served as a solvation and reaction medium for efficiently producing reliable FAB mass spectra and for the partial displacement of the ligands for ease in their identification. (drinksavvyinc.com)
Detection1
  • This reduces solvent load in the plasma without compromising detection limits. (thomassci.com)
Retention1
  • Factors affecting the retention time are length of column, packing material, type of solvent and flow rate of solvent. (majortests.com)
Pesticides1
  • Urinary organophosphate pesticides uses azeotropic codistillation of urine, derivitization, gas chromatography-tandem mass spectrometric method (GC-MS/MS). This method can be used to quantify the six dialkylphosphate human metabolites of organophosphate pesticides in urine. (cdc.gov)
Residue2
Column8
Tetrahydrofuran1
  • Dehydrated BDO is used to make the intermediate Tetrahydrofuran (THF), used as a solvent in the manufacture of a number of products, for example. (outsourcing-pharma.com)
Alcohols1
Mobile phase1
Polymer1