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PermeationPolymersHPLCPolymerViscosityPaper ChromatographyWeightsLiquid chromatographyAffinity chromatographyPolystyreneCharacterizationFiltrationMeasurementSilica gelStationary phasePurificationPolyacrylamide Gel ElectrophorDilute solution viscometryMixturesInfrared spectroscopyHigh molecular weightMoleculesAverage Molecular WeightFormaldehydeMethylMass spectrometrySolventCompoundsWeight distributionMolar massBiochemistryElectrophoresisFTIRSizeInorganicPharmaceuticalAnalysisProteinsSodium azideColumnBufferBiologyLaboratoryAbstract
Permeation45
Polymers8
HPLC1
  • 1985) and in rat urine by high-performance liquid chromatography (HPLC) with ultraviolet (UV) absorbance detection following formation of the 2,4-dinitrophenylhydrazone derivative (Shara et al. (cdc.gov)
Polymer11
  • The laboratory is equipped with several instruments each dedicated to certain eluents and chromatographic conditions that allow molecular weight characterisation of a wide range of polymer types. (smithers.com)
  • Herein, we developed a trialkylsilyl substituted 2D-conjugated polymer with the highest occupied molecular orbital level down-shifted by Si-C bond interaction. (nature.com)
  • Gpc columns perform a separation based on the molecular size of polymer. (web.app)
  • Composed of semipermeable, porous polymer gel beads with well defined range of pore sizes. (web.app)
  • GPC is often used to determine the relative molecular weight of polymer samples as well as the distribution of molecular weights. (sctimst.ac.in)
  • Many mechanical and physical properties of polymer materials, including tensile strength, flexibility, impact resistance, thermal and oxidative aging resistance, and corrosion resistance, are directly related to their molecular weight. (cirs-ck.com)
  • Therefore, investigating the molecular weight and molecular weight distribution of polymer materials is one of the important tasks in evaluating material performance. (cirs-ck.com)
  • However, in the presence of a fluctuating second or even third molecular characteristic, GPC/SEC cannot provide information regarding the polymer molar mass. (tsijournals.com)
  • The entropy controlled retention mechanism is paired with the interaction retention mechanisms to simultaneously identify two molecular features of a complex polymer such as copolymers or polymer blends Adsorption, or the distribution of a solute between a solid surface and a volume of its solution in a mobile phase, is the most prevalent interaction retention mechanism used in linked LC procedures. (tsijournals.com)
  • Basic information on retention mechanisms in synthetic polymer liquid chromatography is provided. (tsijournals.com)
  • If time allows) Thermodynamics of polymer solutions and mixtures (blends). (gwc2.on.ca)
Viscosity3
  • In more recent times, addition of other detection techniques like Static Light Scattering and the Intrinsic Viscosity sensor provide direct measurement of molecular size and weight so that conventional calibration techniques are not required. (cf-alba.com)
  • What is Dilute Solution Viscosity (DSV)? (malvernpanalytical.com)
  • The physicochemical properties, such as gelling or melting temperature, viscosity and gel strength of extracted agarose hydrogels have been measured and compared with the agarose obtained from similar source reported in the literature. (scirp.org)
Paper Chromatography2
Weights3
  • Shimpack gpc 80m mixed gel columns 80mc, 80md mixed gel columns that produce linear calibration curves over a wide range of molecular weights. (web.app)
  • Who wants to quantify molecular weights and sizes? (cf-alba.com)
  • Molecular weight distributions and average molecular weights. (gwc2.on.ca)
Liquid chromatography3
  • Pre-Fractionation of Complex Protein Mixtures for 2-D PAGE Using Reversed Phase Liquid Chromatography Volker Badock and Albrecht Otto 12. (nhbs.com)
  • Liquid chromatography (LC) technologies currently provide the most relevant information on both average values and distributions of molecular characteristics? (tsijournals.com)
  • The similarities and differences of two-dimensional liquid chromatography of low-molecular-mass and polymeric compounds are discussed. (tsijournals.com)
Affinity chromatography1
Polystyrene1
  • Molecules are split in columns packed with porous materials, which could consist of glass beads, polystyrene gels, silica gel, etc. (cf-alba.com)
Characterization2
  • Molecular characterization of polysaccharides dissolved in. (web.app)
  • Production and molecular characterization of tailored citrus pectin-derived compounds. (megazyme.com)
Filtration2
  • Neu3.2 and Neu3.3 formed oligomers as demonstrated by gel filtration chromatography experiments. (unibs.it)
  • POMC IV was subjected to further purification on a Sephadex G-50 gel filtration column. (indexindex.com)
Measurement1
  • However, the understanding of the molecular structure of the coupling agents obtained in this way is currently incomplete, as there are no suitable analytical measurement protocols for them. (fraunhofer.de)
Silica gel6
  • Bare silica gel is an acceptable stationary phase. (tsijournals.com)
  • The chemical attachment of suitable groups, usually C18 alkyl groups, onto a carrier, primarily silica gel, produces the practically applicable volume of LC stationary phase. (tsijournals.com)
  • At 35 °C, two separate liquid chromatographic critical ranges were discovered using non-end-capped silica gel C-18 column packing, poly (methyl methacrylate)s (PMMAs), and mixed eluents including acetonitrile (ACN) and toluene. (tsijournals.com)
  • Separation of dried liquid samples with a liquid solvent and a glass plate covered with a thin layer of silica gel. (majortests.com)
  • Thin layer chromatography is done using a thin uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. (majortests.com)
  • The silica gel is the stationary phase, TLC uses a thin uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. (majortests.com)
Stationary phase1
Purification3
  • Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. (sigmaaldrich.com)
  • Further purification was made by gel chromatography and the homogeneous population of halfmolecules has been characterized by the techniques of small-angle X-ray and neutron scattering. (dtu.dk)
  • The CHO cell line secretes recombinant factor IX into a defined cell culture medium, and the recombinant factor IX is purified by a four-step chromatography purification process that does not require a monoclonal antibody step. (globalrph.com)
Polyacrylamide Gel Electrophor3
  • Large-Format Two-Dimensional Polyacrylamide Gel Electrophoresis - Henry Brzeski, Stephen Russell, Tony G. Sullivan, Richard Somiari & Craig Shriver 14. (nhbs.com)
  • BeneFIX is a single component by SDS-polyacrylamide gel electrophoresis evaluation. (globalrph.com)
  • their molecular masses were determined to be 52.5 and 54 kDa, respectively, by observation of the mobility of the protein bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (wikigenes.org)
Dilute solution viscometry1
  • Finally, we will review our well established Dilute Solution Viscometry (DSV) technology which is often used for product release specifications. (malvernpanalytical.com)
Mixtures3
  • To separate and analyse complex mixtures or solutions which include liquids and solids of both organic and inorganic origins. (sctimst.ac.in)
  • Chromatography is the separation of mixtures into their components as a result of differential distribution of the solutes in order for identification, analyses, purify and/or quantity of the mixture for a mobile medium and for a stationary absorbing medium. (majortests.com)
  • Task 2 Chromatography analysis: Thin layer chromatography Chromatography is used to separate mixtures of substances in to components. (majortests.com)
Infrared spectroscopy1
  • We study the influence of the molecular environment (N2) on the Cs-C2 equilibrium of tetrahydrofuran in the [email protected]2 system utilizing nitrogen matrix isolation infrared spectroscopy. (researchgate.net)
High molecular weight3
Molecules1
  • The radiation emitted is absorbed by organic molecules and converted into energy of molecular vibration. (majortests.com)
Average Molecular Weight1
  • In this study, tailored-made citrus pectin-derived compounds were produced through controlled enzymatic and/or chemical modifications of commercial citrus pectin with different degrees of methylesterification (DM) and similar average molecular weight (M W ). In the first treatment, degradation of the citrus pectin (CP) materials by endo -polygalacturonase (EPG) yielded pectins with average M w 's (between 2 and 60 kDa). (megazyme.com)
Formaldehyde1
  • Formaldehyde has been measured in blood by gas chromatography (GC) in conjunction with mass spectrometry (MS) after derivatization of the formaldehyde to the pentafluorophenylhydrazone (Heck et al. (cdc.gov)
Methyl1
  • Experimental data collected from the literature covered the following systems: bulk homopolymerization of styrene, solution polymerization of styrene, solution polymerization of methyl methacrylate, bulk polymerization of n-butyl acrylate, bulk copolymerization of styrene and n-butyl acrylate. (ndltd.org)
Mass spectrometry2
  • These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. (nhbs.com)
  • In This Edition Chromatography Articles - Faster and automated analysis of aroma compounds in cosmetics Mass Spectrometry & Spectroscopy Articles - Frequently Asked Questions (FAQ) abou. (labmate-online.com)
Solvent2
  • Theory of separation a column is made up of swollen gel particles and the solvent used to swell the gel in a suitable tubular container. (web.app)
  • The thin layer chromatography plate is out into a beaker containing the solvent but making sure that the solvent is below the pencil line. (majortests.com)
Compounds2
  • Most widely used technique for quantitative molecular analysis for the identification and structural analysis of organic compounds. (sctimst.ac.in)
  • This is used to test water pollution and can be used to analyse metal ions and organic compounds in solutions. (majortests.com)
Weight distribution4
Molar mass1
  • Multiangle light scattering (MALS) is a technique for determining, independently, the absolute molar mass and the average size of particles in solution, by detecting how they scatter light. (unist.ac.kr)
Biochemistry1
  • Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. (sigmaaldrich.com)
Electrophoresis2
  • Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis - Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari and Craig D. Shriver 7. (nhbs.com)
  • the pulsed-field gel electrophoresis pattern was indistinguishable from that of the isolates of 5 case-patients. (cdc.gov)
FTIR1
  • FTIR experimental studies are supported by the results of the static density functional theory calculations and Car-Parrinello molecular dynamics simulations. (researchgate.net)
Size1
  • PACT Premier and JCSG+) conducted at two temperatures (typically 20°C and 4°C). A typical drop size consists of 100 nl of protein and 100 nl of precipitant solution. (lu.se)
Inorganic2
  • Site-specific iterations of biological clues or cumulative issues Compared at other models in the F2 Mechanisms of Inorganic and Organometallic thrive for using the solution of the processes of these cells and network or cycle proteins and Modelling interactions about sufficient capsids and genes of PPT sites. (scoutconnection.com)
  • The joint Mechanisms of Inorganic and FY527( solution response indirect recombination set), been to Leu+ by poorsocial line with transformation, was understood by Mammalian class. (scoutconnection.com)
Pharmaceutical1
  • Examples of when chromatography is used are in pharmaceutical companies in order to find out the amount of chemicals used in each new product, hospitals for detection of sugar/blood levels and law enforcement for comparisons of samples found at a crime scene. (majortests.com)
Analysis2
  • Analysis of Membrane Proteins by Two-Dimensional Gels - Michael Fountoulakis, 15. (nhbs.com)
  • Molecular weight determination has wide applications in material research and development, quality control, and failure analysis. (cirs-ck.com)
Proteins2
  • Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
  • These portions of the proteins are probably responsible for the differences observed in substrate specificities, as well as in the different subcellular localization and aggregation features observed in solution. (unibs.it)
Sodium azide2
  • Aqueous buffered solution containing ≤0.09% sodium azide. (bdbiosciences.com)
  • After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage. (vectorlabs.com)
Column6
  • 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity. (vectorlabs.com)
  • 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. (vectorlabs.com)
  • 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. (vectorlabs.com)
  • 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. (vectorlabs.com)
  • Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. (vectorlabs.com)
  • Then the retentate was fractionated by ethanol solution and chromatographed on a DEAE Sepharose Fast Flow anion-exchange column to yield a major fraction (POMC IV) which was eluted by 0.5 M NaCl. (indexindex.com)
Buffer2
  • Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. (sigmaaldrich.com)
  • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. (vectorlabs.com)
Biology1
  • The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed. (sigmaaldrich.com)
Laboratory1
  • All laboratory-based protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. (nhbs.com)
Abstract1
  • abstract = "A half-molecular fragment of α2-macroglobulin has been prepared by reducing and alkylating the inter-subunit disulfide bonds in the tetrameric α2-macroglobulin molecule with 1 mM dithiothreitol (40 min) and 3 mM iodoacetamide (40 min). (dtu.dk)