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  • lipid bilayers
  • It is predicted that the spontaneous insertion of hydrophobic helical proteins into lipid bilayers is mainly driven by a hydrophobic free-energy and interaction of polar and charged side chains of protein with the solvent. (iitb.ac.in)
  • With a consideration of hairpin bent a helix as an 'elliptical frustrum' we have demonstrated that insertion of such bent alpha-helices into the lipid bilayers is an outcome of various free energies like solvation, lipid perturbation, protein immobilization in lipid and packing energy. (iitb.ac.in)
  • Many of these remodeling events involve the reshaping, fission, or fusion of lipid bilayers to generate new organelles, release transport vesicles, or stabilize specific membrane structures. (pnas.org)
  • pathways
  • The dissection of TCR generated signals into calcium and PKC-dependent pathways demonstrates that calcium ionophore is sufficient to induce membrane CD40L yet insufficient for sCD40L production. (boisestate.edu)
  • endocytic
  • Association of host organelles with the vacuolar membranes surrounding intracellular pathogens is a feature restricted to organisms that either never enter the endocytic cascade, or that exit it soon after entry ( Sinai and Joiner, 1997 ). (rupress.org)
  • prediction
  • In view of their properties, such structural motifs are potentially useful for inter-residue contact prediction and protein structure refinement. (mdpi.com)
  • roles
  • Here we show that two dynamin-like proteins, DynA and DynB, play critical roles in this process. (pnas.org)
  • Sequence
  • In the GPS system, the bait protein is fused at the N terminus with an Exg2 GPI signal peptide-anchor sequence, which anchors it in the membrane. (plantphysiol.org)
  • B, The C-terminal GPI signal sequence of the Exg2 protein of yeast. (plantphysiol.org)
  • C, Schematic of the pExg2Met-Dest vector used to express the bait fusion protein, including the N-terminal fusion with the GPI signal sequence (see also Supplemental Fig. S1 ). (plantphysiol.org)
  • The 30-amino acid (aa) NH 2 -terminal sequence of ROP2hc, when fused to green fluorescent protein (GFP), is sufficient for mitochondrial targeting. (rupress.org)
  • distal
  • NPHP4 is stably incorporated into the distal part of the flagellar transition zone, close to the membrane and distal to CEP290, another transition zone protein. (biologists.org)
  • Cell
  • Furthermore, immunoprecipitation of cell surface-associated integral membrane PAM demonstrated that very little integral membrane PAM reached the cell surface during early times of chase. (rupress.org)
  • 50% of the truncated PAM-1 protein reached the cell surface within 3 h. (rupress.org)
  • Additionally, the publicaiton ' Reprint of "Protein and Cell Membrane Iodinations with a Sparingly Soluble Chloroamide, 1,3,4,6-Tetrachloro-3a,6a-Diphenylglycoluril" ' is placed in the Top 100 among other scientific works published in 2012. (citeweb.info)
  • These results demonstrate a new mechanism for tight association of different membrane-bound organelles within the cell cytoplasm. (rupress.org)
  • Although the membrane that surrounds a cell is effective at separating the inside of a cell from the outside environment, certain molecules and ions must enter or leave the cell for it to work correctly. (elifesciences.org)
  • Proteins embedded in the cell membrane, called transporters, ensure this occurs. (elifesciences.org)
  • structural
  • Our results highlight the potential of high-throughput experimental approaches for the identification of protein domains for structural studies. (garvan.org.au)
  • We have extracted an extensive collection of recurrent structural motifs (RSMs), which consist of sequentially non-contiguous structural motifs (4-6 residues), each of which appears with very similar conformation in three or more mutually unrelated protein structures. (mdpi.com)
  • We find that the proteins in our set are covered to a substantial extent by the recurrent non-contiguous structural motifs, especially the helix and strand regions. (mdpi.com)
  • This indicates that the recurrent structural motifs, as we define them, describe recurrent structural patterns that are important for protein stability. (mdpi.com)
  • Electrophoresis
  • Water-soluble membrane proteins may be analyzed by a new, rapid technique that combines electrophoresis on high-resolution sodium dodecyl sulfate (SDS) polyacrylamide gels and immunoelectrophoresis. (sciencemag.org)
  • After separation in the first dimension by electrophoresis in SDS, the proteins are subjected to a second electrophoresis at right angles through a two-layered buffered agarose gel. (sciencemag.org)
  • organisms
  • The protein nephrocystin-4 (NPHP4) is widespread in ciliated organisms, and defects in NPHP4 cause nephronophthisis and blindness in humans. (biologists.org)
  • methods
  • Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science. (beck-shop.de)
  • As in conventional SUS methods, ubiquitin is split between the N-terminal half (Nub) and the C-terminal half (Cub), and the latter is fused with the transcriptional activator complex Protein A-LexA-VP16 (PLV). (plantphysiol.org)
  • Therefore
  • Therefore, these two proteins, which are incorporated into the transition zone independently of each other, define different domains of the transition zone. (biologists.org)
  • structures
  • Protein structures as we observe them in experimentally determined structures reflect the global energy minimum for a given protein. (mdpi.com)
  • 1 ]) have made considerable progress in rapid large-scale determination of protein structures by X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy. (mdpi.com)
  • surfaces
  • The dissolution process, which is the key step of the preparation of SEP, has been followed by scanning electron microscopy (SEM) to observe the changes of the surfaces and thickness of the eggshell membrane (ESM). (blogspot.com)