Loading...
  • Assay
  • HEPES is the recommended buffer for the glutamate binding assay because it prevents binding to non-receptor materials. (mpbio.com)
  • absorbance
  • HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. (mpbio.com)
  • protein
  • HEPES is the buffer of choice in a protein deposition technique in electron microscopy because it did not affect metal substrates. (mpbio.com)
  • Solutions
  • Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used. (mpbio.com)
  • Most solutions of HEPES hemisodium will dissolve in water forming a pH at 7.5 with little to no adjustment. (mpbio.com)
  • appropriate
  • If your cells are prepared and held in a culture media (like RPMI), adding HEPES to the solution is appropriate, as culture medias are typically formulated to buffer in a CO 2 environment. (expertcytometry.com)
  • applications
  • HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. (ufcbio.com)
  • HEPES has been evaluated and shown to be suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isolectric focusing applications. (mpbio.com)
  • cell culture
  • HEPES buffer commonly used in cell culture media. (mpbio.com)
  • The addition of 10-25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. (mpbio.com)