• Currently, manufacturers are focusing on and targeting assays to mutations in the S gene. (who.int)
  • Single nucleotide polymorphisms (SNPs) in the Gγ-globin gene promoter in BCL11A regions and HBS1L-MYB intergenic polymorphism (HMIP) are thought to be associated with Hb F levels. (scirp.org)
  • S-gene dropouts in PCR-based assays have also been used as a quick hack to detect the Alpha variant, for example, but the Omicron variant also shares that signature. (genomeweb.com)
  • 11 SNPs exceeded genome-wide significance with the strongest association with rs12913832 SNP on chromosome 15, mapping to HERC2 gene (p=6.94x10-14). (cdc.gov)
  • Four SNPs exhibited allele-dependent increases in gene expression (rs2287231, rs2513789, rs11571537 and rs2446824). (cdc.gov)
  • Its assays and reagents include Delta Gene and SNP Type Assays, and Access Array Target-Specific Primers and Targeted Sequencing Prep Primers. (nasdaq.com)
  • The design of the primers and the assay is found in Figure 2, which details where the primers attached to the JAK2 gene. (brighthub.com)
  • Thus, the present study was designed to confirm the association of three SNPs in the LOXL1 gene with XFS in a Korean population and to compare results to previous studies of other ethnic groups. (molvis.org)
  • A hybrid qPCR/SNP array approach allows cost efficient assessment of KIR gene copy numbers in large samples. (bvsalud.org)
  • Our approach could potentially be applied to other KIR genes to allow cost effective assaying of gene copy number in large samples. (bvsalud.org)
  • ABSTRACT Single nucleotide polymorphisms (SNPs) in the Interleukin (IL)-28B gene, namely rs12979860, could predict response to pegylated interferon--ribavirin (PR) therapy in hepatitis C virus genotype 1 (HCV-1)-infected patients. (who.int)
  • These newly associated SNPs were associated with the expression levels of RNASET2 at 6q27, of CHRNA9, and of a previously uncharacterized gene at 4p14, respectively. (medscape.com)
  • IDT works directly with 3CR, the originators of the PACE assay, to design the primers. (idtdna.com)
  • How do I design primers for PACE™ genotyping assays? (idtdna.com)
  • Note: To have your primers designed, contact us and provide your SNP coordinates or sequences with identified SNPs. (idtdna.com)
  • This technology uses a unique two-enzyme system coupled with RNA-DNA hybrid primers to interrogate target SNPs. (idtdna.com)
  • A ) Both allele-specific primers query the SNP locus. (idtdna.com)
  • Validation of primer design is particularly important when adopting primers from a previous publication or using a commercially supplied assay. (sigmaaldrich.com)
  • With dye-based detection such as SYBR Green I, primer dimers also affect assay specificity because the nonspecific DNA-binding dye will bind to the primers and be detected along with the desired product. (sigmaaldrich.com)
  • Methods: Primers were designed to target ~ 300-bp regions of both a virulence-associated (cyaA) and non virulence (ushA) genes that contained descriptive SNPs for 4-5 serotypes (3 Enteriditis, Typhi, Typhimurim, Heidelberg, Fetus and Kentucky). (usda.gov)
  • This ValuMix assay format contains two custom BHQplus Probes and two primers combined in a single tube, with a probe to primer ratio of 1:4.5. (biosearchtech.com)
  • citation needed] SNP detection through molecular beacons makes use of a specifically engineered single-stranded oligonucleotide probe. (wikipedia.org)
  • Therefore SNPs and their detection are important for medical research and pharmacology. (bmglabtech.com)
  • The detection of SNPs ( SNP Genotyping ) can be achieved with a number of different methods. (bmglabtech.com)
  • These assays provide a highly flexible technology for detection of polymorphisms within any genome. (thermofisher.com)
  • Please refer to the section on Choosing a PCR screening assay for VOC/VOI detection for further information on how to select the test that suits your needs. (who.int)
  • SNP detection requires high specificity and viral quantification needs both high specificity and sensitivity. (sigmaaldrich.com)
  • Hence, dichlorofluorescein silver nanoparticles (DCF-SNPs) are proposed as an efficient detection system for nanoparticles in agrochemicals for plants. (intechopen.com)
  • Reflecting the needs of clinical research laboratories, such arrays combine high numbers of CNV probes, enabling highly accurate CNV detection, with sufficient SNP probes to reliably detect larger ROH. (mlo-online.com)
  • cartridge antigen detection assay compared to microplate enzyme immunoassay and modified Kinyoun's acid-fast staining technique for detection of Cryptosporidium in fecal specimens. (scienceopen.com)
  • Comprehensive, all-in-one detection of genome-wide CNVs, copy-neutral LOH (cnLOH), SNPs, and indels in one target enrichment capture. (agilent.com)
  • The targeting of genomic regions with high minor allele frequency SNPs allows for detection of copy neutral LOH at 5 Mb resolution. (agilent.com)
  • Contact IDT at [email protected] and provide your SNP coordinates or sequences with identified SNPs. (idtdna.com)
  • SNPs are the most frequent mutations found in DNA sequences. (bmglabtech.com)
  • Create your own assays by submitting target sequences to our secure assay design pipeline using the Applied Biosystems Custom TaqMan Assay Design Tool. (thermofisher.com)
  • Comparison of our mapped SNP sequences with the barley genome revealed that most of the markers (92.4 %) were syntenic. (huji.ac.il)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • The Molecular Inversion Probe assay (Parallele Biosciences, South San Francisco, Calif, USA) [ 6 ] uses probes that can be circularized and contain two sequences complementary to regions adjacent to SNPs in the DNA target in the first step of the genotyping reaction. (hindawi.com)
  • TaqMan Assays can be run on any of our real-time PCR instruments or used as an endpoint assay on other third-party systems, including the Array Tape platform. (thermofisher.com)
  • SNPs with tight Cr4-linkage were further converted to TaqMan assays to confirm their effectiveness as MAS tools. (frontiersin.org)
  • Several applications have been developed that interrogate SNPs by hybridizing complementary DNA probes to the SNP site. (wikipedia.org)
  • In high-density oligonucleotide SNP arrays, hundreds of thousands of probes are arrayed on a small chip, allowing for many SNPs to be interrogated simultaneously. (wikipedia.org)
  • Because SNP alleles only differ in one nucleotide and because it is difficult to achieve optimal hybridization conditions for all probes on the array, the target DNA has the potential to hybridize to mismatched probes. (wikipedia.org)
  • Streamlining both CNV and SNP analysis of the same sample, microarray platforms are now available that incorporate SNP probes onto the aCGH array. (mlo-online.com)
  • Hybridization probes targeted these descriptive SNPs and Luminex MagPlex hybridization protocols were developed and optimized. (usda.gov)
  • We previously developed a highly specific method for detecting SNPs with a microarray-based system using stem-loop probes. (hindawi.com)
  • Are you designing a new assay or considering BHQplus probes for a sequence currently designed for a different probe technology? (biosearchtech.com)
  • For design assistance to achieve optimal results, please contact our assay design service team to redesign your assay with BHQplus probes. (biosearchtech.com)
  • However, allelic and copy number variation in the KIR region effectively mask it from standard genome-wide association studies single nucleotide polymorphism (SNP) probes targeting the region are often discarded by standard genotype callers since they exhibit variable cluster numbers. (bvsalud.org)
  • The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. (wikipedia.org)
  • Genotype data was generated using the Illumina iSelect beadchip assay platform for wheat germplasm lines. (cgiar.org)
  • Genotype frequencies of each SNP were compared according to the XFS phenotypes. (molvis.org)
  • There was no significant association between the genotype frequencies of the three SNPs and the XFS phenotypes. (molvis.org)
  • Based on linkage disequilibrium (LD)-based association mapping used to detect single nucleotide polymorphism (SNP) markers associated with MGR against C. ribicola , MGR in these seed families appears to be controlled by Cr4 or other R genes in very close proximity to Cr4 . (frontiersin.org)
  • These associated SNPs were located in genes involved in multiple molecular mechanisms potentially underlying limber pine MGR to C. ribicola , including NBS-LRR genes for recognition of C. ribicola effectors, signaling components, and a large set of defense-responsive genes with potential functions in plant effector-triggered immunity (ETI). (frontiersin.org)
  • Disease associated SNPs were detected near ODZ3 and CDH17 genes (rs908084, p=8.59x10-9 and rs2514805, p=1.22x10-8, respectively). (cdc.gov)
  • 5.0 × 10 −6 ) of SNPs annotated to four genes. (frontiersin.org)
  • We conclude that PCR-based enrichment of transposon insertions in a TnSeq assay is reliable, but researchers interested in profiling putative essential genes should carefully weigh the number of amplification cycles employed in their library preparation protocols. (microbiologyresearch.org)
  • First, we showed that noise and batch effects in multiplexed qPCR assays are addressed through normalisation and simultaneous copy number calling of multiple genes . (bvsalud.org)
  • Then, we used supervised classification to impute copy numbers of specific KIR genes from SNP signals. (bvsalud.org)
  • SNPs in 6 miRNA processing genes and in 78 miRNAs which target genes known to be important in breast cancer among 906 African American (AA) and 653 European American (EA) cases and Author Manuscript controls enrolled in the Women's Circle of Health Study (WCHS). (cdc.gov)
  • We found a number of SNPs in miRNAs and processing genes in association with breast cancer overall or stratified by estrogen receptor (ER) status. (cdc.gov)
  • A second contributor of variation is single nucleotide polymorphism (SNP), in which two distinct alleles are possible at a single genome position, with each allele appearing at high frequency within the population. (mlo-online.com)
  • The risk alleles of these SNPs were similar to those of other Asian populations, such as Japanese or Chinese, but differed from non-Asian populations, suggesting that still unidentified genetic or environmental factors may contribute to disease expression. (molvis.org)
  • The wheat 90K iSelect SNP genotyping assay was used for genotyping of the RILs, detecting segregation for 16,387 polymorphic markers. (huji.ac.il)
  • The 90K iSelect assay identified 1425 SNP markers, which were polymorphic between the two parents. (usda.gov)
  • The B genome was slightly more polymorphic (57 %) for co-dominant SNP markers than the A genome. (huji.ac.il)
  • We developed 63 high-heterozygosity bull trout polymorphic SNPs and one sex-identification SNP and tested them on range-wide samples. (usgs.gov)
  • Mammoth has incorporated CasDx1 into an assay for SARS-CoV-2 variants on its DETECTR system, which the firm hopes will offer labs a faster, cheaper alternative to sequencing-based viral surveillance. (genomeweb.com)
  • The initial NIOSH R01 grant award (2006-2012) identified candidate variants (snps) linked to Th2 cytokines, antioxidant enzymes, and HLA class I and II antigens. (cdc.gov)
  • These studies are of particular studies aim to identify relevant genetic importance as the knowledge of the susceptibility variants by genotyping up etiology of cancers more prevalent in to 1 000 000 genetic variants (or SNPs). (who.int)
  • SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. (wikipedia.org)
  • SNPs (Single nucleotide polymorphisms) are positions in genomic DNA with a single nucleotide difference between individuals of a species. (bmglabtech.com)
  • A single-tube assay setup allows for routine automation and delivers genotypes with only 90 minutes of cycling time (Figure 1). (idtdna.com)
  • SNP single-nucleotide polymorphism. (cdc.gov)
  • The discovery of novel single nucleotide polymorphisms (SNPs) is critical for plant genotyping in that it helps to provide candidate markers for marker-assisted selection programs in key crops. (thermofisher.com)
  • 372 donors were genotyped using a custom precision medicine single nucleotide polymorphism (SNP) microarray. (nature.com)
  • In collaboration with a team at the University of California, San Francisco, the firm has demonstrated the new CRISPR-associated systems (Cas) enzyme can detect and distinguish multiple SNPs within a single assay. (genomeweb.com)
  • A novel approach to molecular serotyping of poultry-related Salmonella would be to type them based on single nucleotide polymorphisms (SNPs) within the genetic code of these diverse serotypes. (usda.gov)
  • The present study reports the identification of significant single nucleotide polymorphism (SNP) markers associated with super soft trait using single marker trait association and composite interval mapping (CIM) to determine sources of genetic variation for kernel texture conferring the super soft trait. (usda.gov)
  • To study the genetic variation for SKCS HI, the RIL population along with their parental lines was genotyped using a high-density single nucleotide polymorphism (SNP) array. (usda.gov)
  • Finally, the pooling of DNA samples or multiplex PCR strategies for SNP genotyping have also been described in several microarray methods, such as the original arrayed primer extension (APEX) [ 9 - 12 ] or single base extension (SBE-tags) [ 13 , 14 ] assays. (hindawi.com)
  • A total of 13,589 single-nucleotide polymorphisms (SNPs) were selected andused for further association mapping. (cgiar.org)
  • Because of ARMS' ability to detect single nucleotide polymorphisms (SNPs) in heterozygote patients, the technique has been increasingly popular. (brighthub.com)
  • Genotypes of three single nucleotide polymorphisms (SNPs) of LOXL1 (rs1048661, rs3825942, and rs2165241) were analyzed with direct sequencing, and a case-control association study was performed. (molvis.org)
  • Methods: We investigated the value of using 77 breast cancer-associated single nucleotide polymorphisms (SNPs) for risk stratification, in a study of 33 673 breast cancer cases and 33 381 control women of European origin. (kb.se)
  • Single nucleotide polymorphisms (SNPs) in miRNAs and risk of breast cancer have been evaluated in populations of European or Asian ancestry, but not among women of African ancestry. (cdc.gov)
  • We ing practice, different workload, and the use of personal are monitoring single nucleotide polymorphisms (SNPs) protection devices. (cdc.gov)
  • One study conducted a genome-wide association study in 1536 individuals with Graves disease and further evaluated a group of associated single nucleotide polymorphisms (SNPs) in a second set of 3994 cases. (medscape.com)
  • Similarly, when multiple targets are to be detected simultaneously in multiplex reactions, assay conditions must be optimized to detect all targets equally. (sigmaaldrich.com)
  • NEW YORK - Mammoth Biosciences has recently forged a novel SNP-detecting enzyme, dubbed CasDx1, to support multiplex CRISPR-based infectious disease variant testing. (genomeweb.com)
  • Grace Bio-Labs featured at the Multiplex Rapid Assays Workshop in Tempe, AZ. (gracebio.com)
  • Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. (gracebio.com)
  • Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research. (idtdna.com)
  • SNPs are one of the most common types of genetic variation. (wikipedia.org)
  • SNPs can also provide a genetic fingerprint for use in identity testing. (wikipedia.org)
  • This ultra-dense SNP-based genetic map with a high level of synteny to barley provides a useful framework for genetic analyses of important traits, positional cloning and marker-assisted selection, as well as for comparative genomics and genome organization studies in wheat and other cereals. (huji.ac.il)
  • Workshop : Genetic Association Studies: From SNP design to analysis and interpretation. (edu.sa)
  • The Amplifluor ® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of a BMG LABTECH microplate reader. (bmglabtech.com)
  • The rhAmp SNP Genotyping System is a fully integrated genotyping solution that includes an extensive predesigned assay collection, a custom design tool, reagent mixes, and optional synthetic control templates. (idtdna.com)
  • The rhAmp SNP Genotyping System offers enhanced allelic discrimination versus traditional methods. (idtdna.com)
  • We tested all possible pair-wise multiplicative interactions and constructed a 77-SNP polygenic risk score (PRS) for breast cancer overall and by estrogen receptor (ER) status. (kb.se)
  • The GoldenGate assay (Illumina, San Diego, Calif, USA) [ 7 , 8 ] is based on a similar principle but includes two oligonucleotides flanking the SNPs for the genotyping reactions. (hindawi.com)
  • Illumina array: 2.5M SNPs incl. (who.int)
  • Overall, these assays and approaches detect specific mutations or features such as insertions, deletions and point mutations in the SARS CoV-2 genome that are characteristic of a particular VOC/VOI using PCR. (who.int)
  • Because DASH genotyping is measuring a quantifiable change in Tm, it is capable of measuring all types of mutations, not just SNPs. (wikipedia.org)
  • Study both CNVs and mutations for your constitutional studies using one assay. (agilent.com)
  • Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. (wikipedia.org)
  • In contrast to this efficient approach, SNP analysis is usually accomplished using high-resolution SNP microarrays. (mlo-online.com)
  • Genotypes from the sex-identification SNP showed 95% agreement with the field sex identification across 113 samples. (usgs.gov)
  • ABSTRACT This study aimed to estimate the frequency of the SNPs (+45T>G and +276G>T) genotypes and investigate the association between the two SNPs and adiponectin concentration, metabolic parameters and risk of T2DM in the Bahraini population. (who.int)
  • Claims relating to the performance of assays should be verified under the conditions of the study, including test samples, specific reagents and the instrument of choice. (sigmaaldrich.com)
  • However, all assays have a set of optimal conditions and these are dependent on the instrument, selected reagents (buffer conditions), primer concentration and/or annealing temperature (T a ), magnesium concentration and even ramp rate. (sigmaaldrich.com)
  • Its products include preparatory instruments, preparatory analytical instruments, analytical instruments, integrated fluidic circuits (IFCs), and assays and reagents. (nasdaq.com)
  • The map included 1,012 null allele markers, in which only one SNP allele was detected, and the frequency of these markers in the B genome of wild emmer greatly exceeded that of the A genome (69 and 31 %, respectively), which may reflect a greater rate of genomic changes in the B genome. (huji.ac.il)
  • Improved plotting, including a new across assays plot of regions of allelic imbalance for each chromosome and whole genome plots for each assay. (lu.se)
  • We focused on SNPs within or near TACR1/LOC105374811 (chromosome 2) and CTNNA3/DNAJC12 (chromosome 10). (cdc.gov)
  • The molecule is also engineered such that only the probe sequence is complementary to the genomic DNA that will be used in the assay (Abravaya et al. (wikipedia.org)
  • 2003). If the probe sequence of the molecular beacon encounters its target genomic DNA during the assay, it will anneal and hybridize. (wikipedia.org)
  • You can view Archer assays alongside IDT's xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. (idtdna.com)
  • Combined with IDT's universal reporter chemistry, rhAmp SNP Genotyping offers a simple, cost-effective genotyping solution. (idtdna.com)
  • Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. (scienceopen.com)
  • In an attempt to quantify OS in a cell model, we examined OS induced by incubating for 30 min with various free radical generators in PC12 cells by using the dichlorofluorescein (DCF) assay, modified for use by a fluorescent microplate reader. (scienceopen.com)
  • We also discuss the development of homogeneous fluorogenic polymerase chain reaction assays that target phenotypically neutral sites for practical use in casework. (nih.gov)
  • We genotyped the two ADIPOQ SNPs in 140 unrelated T2DM patients and 66 nondiabetic controls using the polymerase chain reaction-restriction fragment length polymorphism assay. (who.int)
  • Quantitative Polymerase Chain Reaction (qPCR) assays address this issue. (bvsalud.org)
  • The increase of interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods. (wikipedia.org)
  • We will talk also about the methods for SNP genotyping and the advantage and disadvantage of each one. (edu.sa)
  • If only the first probe's fluorophore wavelength is detected during the assay then the individual is homozygous to the wild type. (wikipedia.org)
  • Improved removal of noisy and non-informative homozygous SNPs. (lu.se)
  • Since it is usual to run the assay on a selected instrument and under standard buffer conditions, most optimization procedures are focussed on modification of primer binding kinetics using primer concentration or annealing temperature (T a )/melting temperature (T m ). (sigmaaldrich.com)
  • The primer design can be reviewed with respect to the assay design guidance provided in PCR/qPCR/dPCR Assay Design . (sigmaaldrich.com)
  • Whenever primer dimer products are produced and amplified, they divert reaction components away from synthesis of the desired product, thereby reducing assay efficiency and sensitivity. (sigmaaldrich.com)
  • In this application note we describe a homogeneous fluorescence assay based on PCR. (bmglabtech.com)
  • While SNP was not a strong pro-oxidant, SIN-1 was the most potent pro-oxidant among those tested, inducing a 70 times increase of fluorescence at a concentration of 100 microM compared with control. (scienceopen.com)
  • Analysis of CNVs and SNPs is therefore an important part of constitutional genetics research in children displaying signs of developmental disorders. (mlo-online.com)
  • The unique design of these molecular beacons allows for a simple diagnostic assay to identify SNPs at a given location. (wikipedia.org)
  • There are a number of factors that can be altered to obtain optimum assay performance and thereby lead to higher molecular sensitivity, specificity and precision. (sigmaaldrich.com)
  • Designed specifically for molecular cytogenetics research, these new arrays enable the effortless integration of SNP analysis into existing aCGH workflows, providing additional insight into underlying disease mechanisms, at little extra cost. (mlo-online.com)
  • Results: Both assays were found to be highly specific and sensitive towards their respective SNP targets, even when testing samples containing all target SNPs for a given assay. (usda.gov)
  • High duplex stability improves SNP/mismatch discrimination and binding to difficult regions including AT-rich targets. (biosearchtech.com)
  • rhAmp Genotyping Master Mix and rhAmp Reporter mixes are specially formulated for use with rhAmp SNP Assays to deliver enhanced allelic discrimination and signal generation. (idtdna.com)
  • Improve the precision of your PCR-based SNP genotyping with rhAmp SNP Genotyping technology . (idtdna.com)
  • The rhAmp SNP portfolio includes all components needed to generate high-quality genotyping data on the real-time PCR instrument of your choice. (idtdna.com)
  • Schematic representation of a rhAmp SNP Genotyping PCR cycle. (idtdna.com)
  • The impact of dichlorofluorescein silver nanoparticles (DCF-SNPs) on the germination percentage of V. radiata has shown that, the 25% concentration of DCF-SNPs is excellent for the growth of Mung seeds (V. radiata). (intechopen.com)
  • A design pipeline is also available for newly discovered human SNPs or assay designs for other species. (idtdna.com)
  • In addition, we tested previously published SNP assays including 11 species-diagnostic SNPs differentiating bull trout from brook trout and 3 brook trout variable SNPs on a broad set of range-wide samples. (usgs.gov)
  • Bound proteins were purified by DNA affinity precipitation assays (DAPA) and eluted proteins analyzed by mass spectrometry (MS), demonstrating that rs14798008 bound H1 histones. (cdc.gov)
  • Protein crystallization is a key assay for structural studies of proteins. (gracebio.com)
  • Analysis of transcription factor (TF) and ChIP- seq datasets identified overlap of 70 SNPs with TF binding regions of relevant lung cell lines (e.g. (cdc.gov)
  • This pipeline has successfully generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data. (thermofisher.com)
  • We will examine the pipeline of "de novo" SNPs after sequencing of a target DNA. (edu.sa)
  • We propose a more powerful and cost -effective alternative, which combines signals from SNPs with more than three clusters found in existing datasets, with qPCR on a subset of samples. (bvsalud.org)
  • Optimization is required to ensure that the assay is as sensitive as is required and that it is specific to the target of interest. (sigmaaldrich.com)
  • The BAFsegmentation software is available as a stand-alone software package, and will become available as as a plug-in to BASE as the handling of SNP arrays in BASE is developed. (lu.se)
  • There was no association between both SNPs and metabolic parameters. (who.int)
  • This include the use of HapMap data or dbSNP, determination of all the specifications of the SNPs and the prediction of their function. (edu.sa)
  • A shorter BHQplus probe will generate lower background and exhibit a higher signal-to-noise ratio, enhancing the sensitivity of your assay. (biosearchtech.com)
  • Preliminary dichlorofluoresceine nanoparticles (DCF-SNPs) synthesis evidence by noticing the solution color transformed from a light green color to a dark brown color. (intechopen.com)
  • An assay that has been designed such that all desirable design criteria were met ( PCR/qPCR/dPCR Assay Design ) is likely to perform well under a wide range of conditions. (sigmaaldrich.com)
  • When it comes to assay design, we don't believe in a one-dye-fits-all strategy. (biosearchtech.com)
  • For design or technical assistance, please contact our assay design and support team with questions regarding probe design. (biosearchtech.com)
  • A luciferase reporter assay, in A549 cells, identified allele-dependent mRNA transcription. (cdc.gov)
  • Validation provides the data required to justify the continued use of the assay in further research projects 1 . (sigmaaldrich.com)
  • Significance: These data highlight the potential of SNP-based assays to rapidly serotype poultry-related Salmonella enterica. (usda.gov)
  • Five SNP markers on chromosomes 3B and 3Dwere significantly correlated with LP, while nine SNP markers on chromosomes 5A and 5B were significantly associated with AUDPC for SB. (cgiar.org)
  • All three SNPs were significantly associated with XFS. (molvis.org)
  • Redesigning your assay for a BHQplus probe is required if you are using a traditional probe sequence that is longer than 25 bases or that has a predicted melting temperature of 70 °C. Alternatively, consider ordering the sequence as a BHQ Probe (traditional length) or BHQnova Probe (extended length). (biosearchtech.com)