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  • Antibody
  • The gemin2-binding site on SMN protein: accessibility to antibody. (embl-heidelberg.de)
  • The amino-acid residues in SMN required for antibody binding are the same as the five most important contact residues for interaction with gemin2. (embl-heidelberg.de)
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • Mutations
  • p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors. (uniprot.org)
  • BIOGENESIS
  • miRNAs are themselves matured from their long RNA precursors by ribonucleic proteins as part of a 2 step biogenesis mechanism involving RNA polymerase 2. (mirbase.org)
  • RNAi
  • First, a genome-wide RNAi screen for C. elegans SMN modifier genes was undertaken, yielding four genes. (nih.gov)
  • Here we describe a different approach to study SMN function using RNAi to silence SMN expression in cells. (ox.ac.uk)
  • vitro
  • AtPRMT5, an Arabidopsis homolog of human PRMT5, was defined as a type II enzyme for its ability to symmetrically dimethylate histone H4, H2A, and myelin basic protein in vitro ( 22 ). (pnas.org)
  • core snRNP
  • In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. (uniprot.org)
  • PRMT5
  • Sm proteins are released from pICln-PRMT5 complex and bind the SMN complex. (nih.gov)
  • PRMT5 is a type II protein arginine methyltransferase that catalyzes the formation of monomethylarginine and symmetric ω- N G , N ′ G -dimethylarginine (SDMA) ( 1 ). (pnas.org)
  • Non-catalytic component of the 20S PRMT5 -containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. (rcsb.org)
  • recombinant
  • Custom ELISA Kits, Recombinant Proteins and Antibodies can be designed, manufactured and produced according to the researcher's specifications. (mybiosource.com)
  • orthologous
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • Function
  • Conserved genes act as modifiers of invertebrate SMN loss of function defects. (nih.gov)
  • We have used genetic approaches in invertebrate models to identify conserved SMN loss of function modifier genes. (nih.gov)
  • To find genes that modulate SMN function defects across species, two approaches were used. (nih.gov)
  • Drosophila orthologs of two genes, which were identified originally in C. elegans, modified Drosophila SMN loss of function defects. (nih.gov)
  • C. elegans orthologs of twelve genes, which were originally identified in a previous Drosophila screen, modified C. elegans SMN loss of function defects. (nih.gov)
  • Loss of PLS3 orthologs enhances SMN loss of function defects in invertebrates.Previous studies in vertebrates suggested that PLS3 orthologs in C. elegans and Drosophila might modify SMN loss of function defects . (nih.gov)
  • Two monoclonal antibodies (mAbs), MANSMA1 and MANSMA12, have been widely-used in studies of SMN function and their precise binding sites on SMN have now been identified using a phage-displayed peptide library. (embl-heidelberg.de)
  • SMN is shown in the figure as an oligomer as it has been shown to self-associate and it has been suggested that oligomerization is critical for SMN function (see text). (nih.gov)
  • Some research findings indicate that a shortage of this protein impairs the formation and function of axons and dendrites, leading to the death of motor neurons. (nih.gov)
  • These results illustrate that double-stranded RNA can be an effective gene silencing approach even in a protein that is essential for survival and highly expressed, and it could therefore be a valuable tool to study SMN function. (ox.ac.uk)
  • mAbs
  • MANSMA12 immuno-precipitated SMN and gemin2 from HeLa cell extracts as efficiently as mAbs against other SMN epitopes or against gemin2. (embl-heidelberg.de)
  • Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. (abcam.com)
  • precursors
  • B) Polyproteins in which the primary cleavage between the capsid proteins and replication protein precursors is mediated by the 2A translational recoding sequence (CHYSEL). (asmscience.org)
  • human
  • Most miRNAs are grouped into clusters in the human genome or within families that share functions, expression profiles, promotors, or are incorporated into the same ribonucleic protein. (mirbase.org)
  • This SMN epitope is highly-conserved and identical in human and mouse. (embl-heidelberg.de)
  • assays
  • In particular, using cell-based assays we developed, we are searching for compounds that may alleviate the deficiency in the SMN protein and increase the amount of functional SMN protein in cells, as well as for compounds that may improve the survival of cells at low SMN. (upenn.edu)
  • eukaryotic
  • SMN is essential for the viability of most eukaryotic organisms and this has made the generation of animal models of SMA extremely difficult. (ox.ac.uk)
  • Functional
  • Despite the fact that many IRES trans -acting factors (ITAFs) are promiscuous RNA-binding proteins, IRESs exhibit distinct requirements in terms of functional RNA-protein associations. (asmscience.org)
  • SMN relocation to damaged centromeres requires its functional Tudor domain and activity of the H3K79 methyltransferase DOT1L. (embl-heidelberg.de)
  • In particular, splicing defects at the flowering regulator FLOWERING LOCUS KH DOMAIN ( FLK ) in atprmt5 mutants reduce its functional transcript and protein levels, resulting in the up-regulation of a flowering repressor FLOWERING LOCUS C ( FLC ) and consequently late flowering. (pnas.org)
  • fibrillarin
  • Fibrillarin is an essential protein that is well known as a molecular marker of transcriptionally active RNA polymerase I. Fibrillarin methyltransferase activity is the primary known source of methylation for more than 100 methylated sites involved in the first steps of preribosomal processing and required for structural ribosome stability. (embl-heidelberg.de)
  • Here, we show fibrillarin domain conservation, structure and interacting molecules in different cellular processes as well as with several viral proteins during virus infection. (embl-heidelberg.de)
  • methyltransferase
  • Binding of Sm proteins is a prerequisite for hypermethylation of the 7mG cap to 2,2,7-trimethylguanosine (TMG) by the Tgs1 methyltransferase, as well as for exonucleolytic removal of the 3′ trailer sequences. (biologists.org)
  • methylates
  • Here we show that AtPRMT5 methylates a wide spectrum of substrates, including some RNA binding or processing factors and U snRNP AtSmD1, D3, and AtLSm4 proteins, which are involved in RNA metabolism. (pnas.org)
  • In this study, we show that in addition to histones, AtPRMT5 methylates various nonhistone substrates, including RNA processing factors, U snRNP AtSmD1, D3, and AtLSm4 proteins. (pnas.org)