Skeletal myosin heavy chains. Medical search. Frequent questions

Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)
Skeletal muscle is an inherently heterogenous tissue comprised primarily of myofibers, which are historically classified into three distinct fiber types in humans: one "slow" (type 1) and two "fast" (type 2A and type 2X), delineated by the expression of myosin heavy chain isoforms (MYHs). (researchgate.net)
We have previously demonstrated that a layer of skeletal muscle cells in the EOMs demonstrate a longitudinal variation in their myosin heavy chain (MyHC) isoforms. (upenn.edu)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
Myosin Heavy Chain (MHC) isoforms were determined by a validated real-time PCR assay. (staffs.ac.uk)
Multi-step immunofuoroscent analysis of vitamin d receptor loci and myosin heavy chain isoforms in human skeletal muscle. (inpharm.rs)

Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)

Our current studies are designed to identify potential signaling pathways connecting increased TEAD-1 expression in skeletal muscle to activation of genes encoding proteins typically restricted to slow-twitch fibers, as well as satellite cell numbers that exceed homeostatic numbers associated with slow-fibers. (missouri.edu)
This gene is found in a cluster of myosin heavy chain genes on chromosome 17. (antibodypedia.com)

2002). Divergence in species and regulatory role of beta -myosin heavy chain proximal promoter muscle-CAT elements. (missouri.edu)
2001). Multiprotein complex formation at the beta myosin heavy chain distal muscle CAT element correlates with slow muscle expression but not mechanical overload responsiveness. (missouri.edu)

Normalizing for skeletal muscle MHC content and isoform expression predicts myooid forces of ~6-18% of control adult skeletal muscles. (josam.org)
All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)

Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)
The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)
This protein is found in heart (cardiac) muscle and in type I skeletal muscle fibers, one of two types of fibers that make up the muscles that the body uses for movement. (medlineplus.gov)
There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
Overexpression of TEAD-1 in transgenic mouse striated muscles produces a slower skeletal muscle contractile phenotype. (missouri.edu)
Robust skeletal muscle atrophy occurs after burn injury, even in muscles located distally to the site of injury. (utmb.edu)
This was accompanied by retainment of the remodeling capacity in muscles of Snell dwarf mice to increase VCAM-1 protein levels and a shift in myosin heavy chain (MHC) isoform distribution with training. (cdc.gov)

Zeb1 and Tle3 are trans-factors that differentially regulate the expression of myosin heavy chain-embryonic and skeletal muscle differentiation. (mpg.de)
Three embryonic class II myosin heavy chains (MYHs) were cloned from the common carp ( Cyprinus carpio L.), MYH emb1 ,MYH emb2 and MYH emb3 . (biologists.com)
Recognises the embryonic myosin heavy chain C-terminal region (light meromyosin (LMM) fragment). (cancertools.org)

The protein is denoted as MyHC-M, for myosin heavy chain-masticatory. (johnhawks.net)
RGX365-treated myotube diameters and myosin heavy chain (MyHC) expression levels were analyzed using immunofluorescence. (bvsalud.org)

Myosin-8 is a protein that in humans is encoded by the MYH8 gene. (wikipedia.org)

The article Skeletal Muscle - Structure and Histology provides a review of normal skeletal muscle histology and ultrastructure, including the histologic appearance of normal muscle with some of the various stains that are used for the processing of muscle biopsies. (medscape.com)

Mutations in the MYH7 gene cause myosin storage myopathy. (medlineplus.gov)
The MYH7 gene provides instructions for making a protein known as the cardiac beta (β)-myosin heavy chain. (medlineplus.gov)
Mutations in the MYH7 gene lead to the production of an altered cardiac β-myosin heavy chain protein, which is thought to be less able to form thick filaments. (medlineplus.gov)
Tajsharghi H, Thornell LE, Lindberg C, Lindvall B, Henriksson KG, Oldfors A. Myosin storage myopathy associated with a heterozygous missense mutation in MYH7. (medlineplus.gov)

To test whether complete removal of skeletal muscle tissue affects the spatiotemporal relationship between myogenesis and angiogenesis during regeneration, subthreshold volumetric muscle loss was created with a biopsy punch (diameter, 2 mm) through the center of the gluteus maximus (GM) in adult mice. (biomedcentral.com)
A second area if interest is how initial satellite cell number is determined during skeletal muscle formation (myogenesis). (missouri.edu)

Cardiac β-myosin heavy chain is the major component of the thick filament in muscle cell structures called sarcomeres . (medlineplus.gov)
Deplection of lean body mass is one of the main consequences of cachexia which involves not only skeletal muscle but also affects cardiac proteins, resulting in alterations in heart performance. (spandidos-publications.com)

2009). IGF-I activates the mouse type IIb myosin heavy chain gene. (missouri.edu)
This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. (antibodypedia.com)
This gene is predominantly expressed in fetal skeletal muscle. (antibodypedia.com)

The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS. (uams.edu)
Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. (antibodypedia.com)
May be a homodimer, which associates with multiple calmodulin or myosin light chains. (lu.se)

One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)
Evidently, a population of small myofibers does exist that do not undergo regeneration in aged skeletal muscle, perhaps accounting for the disparity observed between morphological adaptation and functional performance. (cdc.gov)
Following punch biopsy of adult skeletal muscle, regenerating microvessels span the wound and become perfused with blood prior to myofiber regeneration. (biomedcentral.com)
Notably, one month of oral administration of RGX365 to 23-month-old sarcopenic mice improved muscle fiber size and the expression of skeletal muscle regeneration-associated molecules. (bvsalud.org)
Severe burn injury induces skeletal muscle regeneration and myonuclear apoptosis. (utmb.edu)

The protein product of MYH16 is one of the heavy chain myosins, a kind of protein that works with actin to enable muscle fibers to contract. (johnhawks.net)
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. (antibodypedia.com)
The sarcomeres contain a number of proteins, including alpha actinin, which is the major constituent of the Z band, and actin and myosin, which are the major components of the thin and thick filaments, respectively. (medscape.com)

Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)

This condition is characterized by the formation of protein clumps, which contain a protein called myosin, within certain muscle fibers. (medlineplus.gov)
The altered proteins accumulate in type I skeletal muscle fibers, forming the protein clumps characteristic of the disorder. (medlineplus.gov)
Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. (abcam.cn)
In addition to the increased muscle protein degradation found during cancer growth, the presence of the tumor also induces an increased rate of DNA fragmentation in skeletal muscle in both rats and mice ( 8 ). (spandidos-publications.com)

The increases were paralleled by increases in myosin heavy chain type 1 vastus lateralis muscle fiber cross-sectional area (+14%, P=0.031) and proportion (+17%, P=0.015), thigh lean mass (+4%, P=0.001), capillary-to-fiber ratio (+14%, P=0.003), and cytochrome c oxidase activity. (galileo-training.com)
Myosin immunohistochemistry and fiber adaptation following stretch-shortening contraction loading: influence of glutathione and age. (cdc.gov)
Vullhorst D, Klocke R, Bartsch JW, Jockusch H. Expression of the potassium channel KV3.4 in mouse skeletal muscle parallels fiber type maturation and depends on excitation pattern. (uni-bielefeld.de)
Fiber typing human skeletal muscle with fluorescent immunohistochemistry. (uams.edu)
A major interest of my lab is to better understand the mechanism(s) by which skeletal muscle fiber type composition is regulated. (missouri.edu)

Treatment with β 2 -adrenergic agonists results in skeletal muscle hypertrophy ( 11 - 14 ), while they cause a reduction in the body fat content ( 15 , 16 ). (spandidos-publications.com)
The cellular mechanisms by which contractile activity stimulates skeletal muscle hypertrophy are beginning to be elucidated and appear to include activation of the phosphatidylinositol 3-kinase signaling substrate mammalian target of rapamycin (mTOR). (elsevierpure.com)
Satellite cells are considered adult skeletal muscle stem cells that are responsible for the majority of post-birth skeletal muscle growth (maturation and hypertrophy) and adult skeletal muscle homeostasis. (missouri.edu)

Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
Skeletal muscle is a target tissue for anabolic steroids. (frontiersin.org)
Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)

ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes ( ab277612 ). (abcam.cn)
Purified foetal human skeletal muscle myosin. (cancertools.org)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)
Myosin content of individual human muscle fibers isolated by laser capture microdissection. (uams.edu)
This article describes the structure, histologic features, and ultrastructural features of normal adult human skeletal muscle and addresses the results of improper specimen handling during muscle biopsy. (medscape.com)

To examine gender-dimorphic regulation of skeletal muscle proteins between healthy control and STZ-induced diabetic rats of both genders, we performed differential proteome analysis using two-dimensional electrophoresis combined with mass spectrometry. (karger.com)
Proteomic analysis of skeletal muscle identified a total of 21 proteins showing gender-dimorphic differential expression patterns between healthy controls and diabetic rats. (karger.com)

We conclude that KV3.4 expression in mouse skeletal muscle is regulated by the pattern of excitation. (uni-bielefeld.de)
Violin plots show distribution of expression levels for Myosin heavy chain (SMED30020435) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)

MYH2 (Myosin-2) is associated with muscle contraction and is required for cytoskeleton organization. (thermofisher.com)

We hypothesize that the remaining force deficit is due to cellular and sub-cellular disorganization-Myooids lack the density and organization of sarcomeric arrays seen in skeletal muscle. (josam.org)

The purpose of this study was to characterize the growth and remodeling molecular signaling response in aged skeletal muscle following one month of 'resistance-type exercise' training. (cdc.gov)

The activity of skeletal muscle stem cells, satellite cells, acutely following a severe burn is unknown and may contribute to the recovery of lean muscle. (utmb.edu)

Acute injury to skeletal muscle damages myofibers and fragment capillaries, impairing contractile function and local perfusion. (biomedcentral.com)

Myosin storage myopathy is a condition that causes muscle weakness (myopathy) that does not worsen or worsens very slowly over time. (medlineplus.gov)
The signs and symptoms of myosin storage myopathy usually become noticeable in childhood, although they can occur later. (medlineplus.gov)
Myosin storage myopathy is a rare condition. (medlineplus.gov)
It is unclear how these changes lead to muscle weakness in people with myosin storage myopathy. (medlineplus.gov)
Armel TZ, Leinwand LA. Mutations in the beta-myosin rod cause myosin storage myopathy via multiple mechanisms. (medlineplus.gov)

Skeletal muscle constitutes 40% of the body's mass and is designed to generate force for locomotor activity, postural maintenance, and plays an important role in the regulation of systemic energy balance. (missouri.edu)

The delayed rectifier potassium conductance in the sarcolemma and the transverse tubular system membranes of mammalian skeletal muscle fibers. (uni-bielefeld.de)
This property is primarily attributable to satellite cells which comprise a small population of quiescent mono-nucleated cells which first appear during late fetal life, and which reside between the basal lamina and the surface (sarcolemma) of mature skeletal muscle cells. (missouri.edu)

Dm provides information on skeletal muscle stiffness, morphological and structural changes [ 2 - 4 ]. (plos.org)

Here, we consider the implications of imperfect treatment administration, focusing specifically on outcomes for skeletal muscle. (jci.org)

Such lack of clarity can be attributed to difficulty in isolating the individual events responsible for alterations in skeletal muscle, most of which occur simultaneously, among the multiple age-associated changes and co-morbidities associated with advanced age. (frontiersin.org)

Targeted deletion of Kcne3 impairs skeletal muscle function in mice. (uni-bielefeld.de)

Myooids from aged rats generated greater normalized force than myooids from adult rats, but only 1-3% of that for adult skeletal muscle controls. (josam.org)
The myooids generated specific forces of ~0.1-3% of skeletal muscle from control adult rats. (josam.org)
PURPOSE: The purpose of this research was to identify the influence dietary supplementation with a glutathione antagonist (L-Buthionine Sulfoximine (BSO)) has on the adaptability of skeletal muscle during chronic high-intensity mechanical loading via stretch-shortening contractions (SSCs) in young and old rats. (cdc.gov)

Anatomy10

Organisms3

Diseases1

Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes10

Information Science3

Isoforms8

Explanted skeletal muscle1

Genes2

Beta-myosin heavy2

Isoform expression2

Muscles8

Embryonic3

MyHC2

MYH81

Normal skeletal muscle1

MYH74

Myogenesis2

Cardiac2

Gene3

LIGHT CHAINS3

Regeneration5

Actin3

Myoblasts2

Protein4

Fiber5

Hypertrophy3

Tissue4

Human5

Proteins2

Expression2

MYH21

Sarcomeric1

Molecular1

Stem cells1

Contractile1

Myopathy5

Regulation1

Sarcolemma2

Morphological1

Outcomes1

Alterations1

Mice1

Rats3