• Effects of mechanical forces exerted on mammalian skeletal muscle cells during development were studied using an in vitro model to unidirectionally stretch cultured C2C12 cells grown on silastic membrane. (duke.edu)
  • A much higher percentage of alternatively spliced transcripts are found during differentiation of human myocytes in vitro. (jci.org)
  • Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
  • In vitro studies have shown that the active form of vitamin D, calcitriol, acts in myocytes through genomic effects involving VDR activation in the cell nucleus to drive cellular differentiation and proliferation. (hindawi.com)
  • and [3] therapeutic innovation - FMOD reprogrammed (FReP) multipotent cells can be easily differentiated into skeletal myocytes and osteoblasts in vitro and in vivo, respectively, and will have significant clinical value in regenerative medicine to establish skeletal muscle and bone by autologous cells. (ca.gov)
  • Further investigation of the myometrial and maternal peripheral response to uterine contractions in-vitro and in-vivo was also made with particular reference to the role of inflammation and myocyte damage. (gla.ac.uk)
  • Three-dimensional engineering of skeletal muscle is becoming increasingly relevant for tissue engineering, disease modeling and bio-hybrid robotics, where flexible, versatile and multidisciplinary approaches for the evaluation of tissue differentiation, functionality and force measurement are required. (ibecbarcelona.eu)
  • Troponin T (shortened TnT or TropT) is a part of the troponin complex, which are proteins integral to the contraction of skeletal and heart muscles. (wikipedia.org)
  • In this review, we will specifically address the effect of vitamin D on skeletal muscles and its clinical implications, especially frailty and the risk of fall. (hindawi.com)
  • Glycogen is a complex multibranched polysaccharide that acts as energy storage in the human body and is formed mainly in the liver and skeletal muscles. (medscape.com)
  • Skeletal muscles-which enable animals to move-are made up of large elongated muscle cells that span the entire length of the muscle. (elifesciences.org)
  • Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)
  • Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)
  • These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
  • Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • Proper differentiation and maturation of myocytes is demonstrated by the expression of key myo-proteins using immunocytochemistry and analyzed by confocal microscopy, and the functionality assessed via electrical stimulation and analysis of contraction kinetics. (ibecbarcelona.eu)
  • Initially, her lab focused on regulation of the biosynthesis and assembly of sodium pump subunits and molecular mechanisms controlling sodium pump isoforms in kidney, cardiac and skeletal muscle by potassium, hormones, and other factors under normal conditions and during human heart failure. (usc.edu)
  • Troponins are protein molecules that are part of cardiac and skeletal muscle. (medscape.com)
  • Akt2 expression was low in a multipotent fibroblast cell line, but it was upregulated when these cells were transformed with Myod and induced to differentiate into myocytes. (nih.gov)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Similarly, skeletal muscle pericytes can support endothelial cell organization during angiogenesis and vasculogenesis, but can also differentiate in skeletal myocytes. (bris.ac.uk)
  • 95-99% pure populations of heterokaryons formed between undifferentiated rat myoblasts and differentiated chick myocytes were obtained. (elsevierpure.com)
  • Wright, WE 1981, ' Synthesis of rat myosin light chains in heterokaryons formed between undifferentiated rat myoblasts and chick skeletal myocytes ', Journal of Cell Biology , vol. 91, no. 1, pp. 11-16. (elsevierpure.com)
  • Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
  • ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
  • Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
  • Skeletal myoblasts are available from both male and female donors. (zen-bio.com)
  • These include bone marrow-derived cells, peripheral blood derived stem cells and skeletal myoblasts (3). (escardio.org)
  • The resistant state of skeletal myoblasts to ischemia renders these cells suitable candidates for repair of chronically infracted and failing heart (5). (escardio.org)
  • Myoblasts retain skeletal muscle properties and couple only sporadically to resident cardiomyocytes precluding synchronized contraction as mechanism of benefit (8). (escardio.org)
  • These heterokaryons synthesize the light chain of chick myosin and both embryonic and adult light chains of rat skeletal myosin. (elsevierpure.com)
  • Skeletal muscle pericytes also play central roles in the development, homeostasis and ageing of their native tissue and play a central role in diabetes-related endothelial dysfunction. (bris.ac.uk)
  • Thus, GLP-1 and insulin may regulate skeletal and cardiac muscle endothelial surface area and nutrient delivery under physiological conditions. (medscape.com)
  • In this work, we used a high-throughput chemical biology screen to identify a small-molecule probe, SBI-477, that coordinately inhibited triacylglyceride (TAG) synthesis and enhanced basal glucose uptake in human skeletal myocytes. (uthscsa.edu)
  • Depleting MondoA in myocytes reproduced the effects of SBI-477 on glucose uptake and myocyte lipid accumulation. (uthscsa.edu)
  • The differentiated myocytes also respond to insulin stimulated glucose uptake. (zen-bio.com)
  • Alternative splicing eliminates exon 2 in 0.6-2% of AMPD1 mRNA transcripts in adult skeletal muscle. (jci.org)
  • Stem cells were used in these studies, but pleuropotentiality is not requisite as adult and immature myocytes as well as skeletal muscle each have their proponents. (jwatch.org)
  • Introduction: Insulin-sensitive tissues such as liver and skeletal muscle modify their gene expression under conditions of obesity-induced insulin resistance, and some of these gene products may be released to maintain glucose homeostasis. (endocrine-abstracts.org)
  • ATP from glycolysis is required for normal sodium homeostasis in resting fast-twitch rodent skeletal muscle. (aamc.org)
  • The control of gene expression during terminal myogenesis was explored in heterokaryons between differentiated and undifferentiated myogenic cells by analyzing the formation of species specific myosin light chains of chick and rat skeletal muscle. (elsevierpure.com)
  • Control homokaryons formed by fusing undifferentiated cells to themselves did not synthesize skeletal myosin light chains. (elsevierpure.com)
  • These results identify a key role for MondoA-directed programs in the coordinated control of myocyte lipid balance and insulin signaling and suggest that this pathway may have potential as a therapeutic target for insulin resistance and lipotoxicity. (uthscsa.edu)
  • Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
  • The mechanistic links between lipid accumulation in myocytes and insulin resistance are not completely understood. (uthscsa.edu)
  • Skeletal muscle is an important site of insulin-stimulated glucose disposal and often the site of insulin resistance in obesity. (zen-bio.com)
  • This study aimed to identify mRNAs encoding liver and skeletal muscle peptides that have the potential to regulate β-cell function by binding to islet GPCRs, and to quantify changes in expression of these liver and muscle mRNAs in obese. (endocrine-abstracts.org)
  • On the other hand, it is formed and accumulated in the liver and skeletal muscle when blood glucose is elevated after food intake or during the "fed state. (medscape.com)
  • Enzyme deficiency results in glycogen accumulation in tissues, especially liver and skeletal muscle cells. (medscape.com)
  • it is defined as a severe systemic manifestation (eg, rhabdomyolysis) of trauma and ischemia involving soft tissues, principally skeletal muscle, as a result of prolonged severe crushing. (medscape.com)
  • Expression levels of mouse Akt2 mRNA and Akt2 protein varied among tissues, with the highest levels in skeletal muscle. (nih.gov)
  • However, the ubiquitary nature of vitamin D receptor (VDR) suggests potential for widespread effects, which has led to new research exploring the effects of vitamin D on a variety of tissues, especially in the skeletal muscle. (hindawi.com)
  • Slow skeletal troponin T1, TNNT1 (19q13.4, 191041) Cardiac troponin T2, TNNT2 (1q32, 191045) Fast skeletal troponin T3, TNNT3 (11p15.5, 600692) The 99th percentile cutoff for cardiac troponin T (cTnT) is 0.01 ng/mL. (wikipedia.org)
  • Troponin C1 is skeletal and cardiac type whereas troponin C2 is skeletal type. (nih.gov)
  • Troponin C1 is a BIOMARKER for damaged or injured CARDIAC MYOCYTES and mutations in troponin C1 gene are associated with FAMILIAL HYPERTROPHIC CARDIOMYOPATHY. (nih.gov)
  • Transfection of C2C12 mouse myocytes with a luciferase reporter under the control of the IL-6 promoter indicated each immunomodulator enhanced IL-6 promoter activity. (dovepress.com)
  • The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)
  • Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)
  • ioSkeletal Myocytes are created through direct reprogramming of human iPSCs (induced pluripotent stem cells) using bit.bio's breakthrough cell coding technology opti-ox™ . (azolifesciences.com)
  • Understanding their reparative potential and how pathologicalconditions associated with ischemia affect these cells is crucial in order to fullyexploit their therapeutic potential.The following chapter will therefore address the biological characteristics ofadventitial and skeletal muscle pericytes in health and tissue-specific diseases. (bris.ac.uk)
  • Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
  • Metabolic Remodeling of Human Skeletal Myocytes by Co-cultured Adipocytes Depends on the Lipolytic State of the System. (duke-nus.edu.sg)
  • Moreover, the rapid rise of new therapeutics with the potential to cause SKM injury, such as immunotherapies and targeted therapies, as well as new treatments (such as gene therapy) specifically targeting muscular or neuromuscular diseases, demands an improved clinical strategy to identify myocyte injury and enable appropriate and timely clinical intervention. (degruyter.com)
  • The nicotinic receptors are cylindrically-shaped proteins imbedded in synaptic walls that act as chemically-controlled sodium channels (also called ligand-gated sodium channels) that penetrate through the cell walls of post-synaptic nerves and myocytes at the skeletal neuromuscular junctions. (cdc.gov)
  • Skeletal neuromuscular junctions. (cdc.gov)
  • Skeletal muscle tissue (SKM) may be damaged due to mechanical, metabolic, and exertional causes. (degruyter.com)
  • Human IDI2 is expressed only in skeletal-myocellular peroxisomes and instant spikes in isoprene exhalation during muscle activity underpins its origin from muscular lipolytic cholesterol metabolism. (nature.com)
  • Immunohistochemical analysis of paraffin-embedded human skin (left) or skeletal muscle (right) using using α-Actinin 4 (D7U5A) Rabbit mAb. (cellsignal.com)
  • Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human skeletal muscle shows low positivity in myocytes as expected. (novusbio.com)
  • It's the first product of its kind that provides physiologically relevant human skeletal muscle cells that are reliable, reproducible and consistent at scale. (azolifesciences.com)
  • Previously, acquiring skeletal myocytes for research has either been from animal models, which do not always behave like human muscle cells, or by using primary human cells with methods that produce only small cell quantities. (azolifesciences.com)
  • We have seen first-hand that ioSkeletal Myocytes eliminate this cumbersome, time-consuming process, and quickly provide reliable and consistent access to large quantities of human myocytes, which can be used for high-throughput screening. (azolifesciences.com)
  • The product provides functional and consistent human cells which can be used to study the physiology of human skeletal muscle cells and their response to potential drug candidates. (azolifesciences.com)
  • bit.bio's human ioSkeletal Myocytes will increase translatability of research findings so can play a significant role in reducing the average cost and time spent on drug development and increase the chances of new treatments making it through the clinic and to patients. (azolifesciences.com)
  • This works presents a 3D-printed platform of bioengineered human skeletal muscle which can efficiently model the three-dimensional structure of native tissue, while providing information about force generation and contraction profiles. (ibecbarcelona.eu)
  • Osteoporosis, a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue with a consequent increase in bone fragility, occurs frequently, particularly in older women. (springer.com)
  • Building on these findings, the lab addressed the coordinated and concerted control of extracellular potassium by skeletal muscle, kidney and gut in response to altered metabolic and electrolyte demands and have begun to use these findings to address the mechanisms responsible for the cardiovascular benefit of raising dietary K/Na ratio. (usc.edu)
  • The present findings provide novel insights on inflammation-induced stimulation of IL-6 promoter activity in skeletal muscle which is an important but non-traditional component of the innate immune system. (dovepress.com)
  • In addition to the temporal and contractile association with the inflammatory response, our data suggest inflammation occurs in response to myocyte cellular damage regardless of mode of damage e.g. contractile or chemically induced. (gla.ac.uk)
  • Experiments were conducted to evaluate the effects of glycolytic inhibition on cellular Na + and K + concentrations and lactate production in rat skeletal myocytes. (aamc.org)
  • Rat skeletal muscle fibers were extracted and incubated in normal media (control), glucose-free media (G(-)), and glucose-free media with various concentrations of the glycolytic inhibitor iodoacetate (IAA). (aamc.org)
  • Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
  • Iron plays a crucial role in the delivery and utilization of oxygen, and "cells with high-energy demands, including skeletal and cardiac myocytes, are particularly sensitive to depleted iron stores," said Gregory D. Lewis, MD , of the pulmonary critical care unit of Massachusetts General Hospital, Boston, and his associates. (the-hospitalist.org)
  • The differentiated parental cells were mononucleated muscle cells (myocytes) that were obtained from primary cultures of emhryonic chick thigh muscle by blocking myotube formation with EGTA and later incubating the postmitotic cells in cytochalasin B. Heterokaryons were isolated by the selective rescue of fusion products between cells previously treated with lethal doses of different cell poisons. (elsevierpure.com)
  • The purpose of the present study was to examine the involvement of NF-κB in the induction of the IL-6 promoter in skeletal muscle cells by endotoxin (lipopolysaccharide [LPS]), tumor necrosis factor alpha (TNFα) or IL-1α. (dovepress.com)
  • The principle challenge today is to produce enough regenerative cells through a simple, consistent process that bypasses ethical and immunological barriers and avoids genome alteration for in vivo skeletal muscle and bone construction without causing tumor formation. (ca.gov)
  • Skeletal muscle fibre formation requires a coordinated programme of morphological and biochemical changes in the differentiating cells. (elifesciences.org)
  • The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)
  • In clinical practice, as well as during drug development and in clinical trials, the diagnosis of SKM injury can be challenging due to the variable symptomology, reliance on patient-self reporting, and lack of highly specific biomarkers, such as those available for cardiac myocyte injury (i.e., cardiac troponins) [ 3 ]. (degruyter.com)
  • These results demonstrate that, under conditions simulating mammalian long bone growth, cultured myocytes respond to mechanical forces by lengthening and orienting along the direction of stretch. (duke.edu)
  • In the central nervous system, lower motor neurons are selectively affected, whereas pathology of patients and animal models also indicates involvement of skeletal muscle including loss of fasttwitch type 2 fibres and increased slow-twitch type 1 fibres, together with a glycolytic-to-oxidative metabolic switch. (kennedysdisease.org)
  • Access to ioSkeletal Myocytes will supercharge research and drug discovery for musculoskeletal and metabolic conditions. (azolifesciences.com)
  • For insulin to exert its metabolic effects in muscle and for nutrients to be used by the myocytes, insulin and nutrients first have to be delivered to the capillaries bathing the myocytes and then transported through the vascular wall to reach muscle interstitium. (medscape.com)
  • However, in fact, just as Jatene ventriculoplasty, skeletal muscle wrap and ventricular restraint devices, this therapeutic option may be more suitably and euphemistically categorized as of potential value only for very highly selected patients. (jwatch.org)
  • Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch. (duke.edu)
  • Adventitial and skeletal muscle pericytes possess intrinsic reparative capacity, both in situ where they participate to tissue repair and remodelling and when used as a cell therapy option. (bris.ac.uk)
  • Retrieved on October 03, 2023 from https://www.azolifesciences.com/news/20211027/ioSkeletal-Myocytes-7c-New-product-launch.aspx. (azolifesciences.com)
  • A) is important for maintaining normal Na + and K + levels in skeletal muscle. (aamc.org)