Skeletal muscle tissue skeletal muscle stem cells clone cells. Medical search. Frequent questions

One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
ENGLISH ABSTRACT: Introduction: Adult mammalian tissue regeneration recruits progenitor stem cells. (sun.ac.za)
Fibroblast Growth Factors (FGFs) are fundamental in embryonic development but also in adult skeletal muscle regeneration from injury or pathology. (sun.ac.za)
Understanding the role of specific members of this growth factor family could assist in improving the understanding of their influence on the regeneration sequence in skeletal muscle. (sun.ac.za)
Stem cells are considered the most promising source for such meat, retaining as they do the capacity to transform into the required tissues, and the scientists pointed to satellite cells, which are the natural muscle stem cells responsible for regeneration and repair in adults. (livescience.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
Examples of projects this cohort include determining the effects of muscle progenitor cells on white blood cells function in mice with diet-induced obesity, evaluating migration of mesenchymal stem cells into biomaterials, and studying whole-body regeneration in a marine invertebrate, among others. (ca.gov)
But in mammals, including humans, some tissues (e.g., heart muscle) are more resistant to regeneration. (cshlpress.com)
The contributors discuss the dramatic molecular and cellular changes that occur when a regeneration program is initiated, the progenitor cells and morphogenic signals involved, the formation of a blastema, the roles of reprogramming and polyploidy, the diversity of cell fates, the integration of new structures with existing body parts, and our current understanding of why some structures are more resistant to regeneration than others. (cshlpress.com)
The importance of technologies (e.g., single-cell RNA-seq) that have been instrumental in deciphering various aspects of regeneration in recent years is emphasized throughout. (cshlpress.com)
Several chapters are also devoted to regeneration in specific human organs-the skin, retina, heart, lung, pancreas, liver, skeletal muscle, and intestine-and examine possibilities for therapeutically replacing injured or diseased structures and for managing age-related declines in function. (cshlpress.com)
This volume is therefore essential reading for molecular, cell, and developmental biologists studying regeneration in animals, as well as for all interested in the development of regenerative therapies for clinical application. (cshlpress.com)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
In addition, the urethral caliber, speed of urothelial regeneration, content of smooth muscle, and vessel density were significantly improved in the group with autologous USC-seeded SIS. (biomedcentral.com)

The MC-813-70 antibody reacts with stage-specific embryonic antigen-4 (SSEA-4), a glycolipid carbohydrate antigen expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), undifferentiated embryonic stem (ES) and induced pluripotent stem (iPS) cells, a subset of mesenchymal stem cells, and rhesus monkey ES cell lines. (stemcell.com)

Somatic cell nuclear transfer (SCNT) is a technology applied in cloning, stem cell research and regenerative medicine. (asu.edu)
Graduates of our program are directly strengthening stem cell research and regenerative medicine by performing fundamental investigations in both academic laboratories (as research associates and doctoral students) and for-profit companies, developing and manufacturing regenerative medicine products at biotech companies, and participating in clinical-trial organization. (ca.gov)

The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the embryo. (wikipedia.org)

We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
The central focus of his research is to understand how changes in the tumor microenvironment and stem cell niche regulate the development of cancer and normal hematopoietic tissues, how interactions between tumor cells, cancer stem cells and the tumor immune microenvironment can drive the progression of tumors and resistance to chemotherapies. (socalflow.org)

After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
In order to distinguish the rate of proliferation between different PHM clones, a comparative index (CI) was established using the cell cycle and total RNA data of the two PHM clones. (sun.ac.za)
Finally, FGF6 and FGF2, both individually and sequentially, were used to treat quiescent myoblasts to determine their involvement in activation and proliferation with the use of cell cycle analysis and mRNA assessment of ki67, p21, myf5, and MyoD. (sun.ac.za)
Results and conclusion: The development of the CI was successful in determining the difference in proliferation rate for the different clones. (sun.ac.za)
However, FGF2 did impede the cell cycle inhibition factor p21, indirectly influencing proliferation. (sun.ac.za)
The inverse sequential treatment order did not demonstrate any significant effect on both activation and proliferation of the quiescent cells. (sun.ac.za)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
Balancing the rate of differentiation and proliferation in developing tissues is essential to produce organs of robust size and composition. (biologists.com)
The master kinase LKB1 is a key regulator of several cellular processes, including cell proliferation, cell polarity and cellular metabolism. (sdbonline.org)
In vitro studies also revealed that GFAT1 promoted the proliferation, cell cycle progression, migration and invasion of HCC cells. (oncotarget.com)

They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
Most studies investigating satellite cells in vitro use already activated satellite cells, called myoblasts. (sun.ac.za)
Methods: Isolated satellite cells from human muscle biopsies were expanded in vitro creating primary human myoblast (PHM) clones. (sun.ac.za)
In insect, Drosophila melanogaster ryanodine receptor ( DmRyR ) cDNA was cloned from lava and the physical features of DmRyR single channel were characterized with in vitro overexpression system [ 16 ]. (biomedcentral.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Results: Mesoangioblast clones express Sca-1 and Flk-1 and differentiate into smooth and skeletal muscle, glial cells and adipocytes in vitro. (illinois.edu)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
Consistent with this, transcriptomic analysis revealed deregulation of BCL2 family members, including decrease of anti-apoptotic long form of myeloid cell leukemia-1 (MCL1) expression, as crucial for "priming" MM cells for Venetoclax activity in vitro and in vivo, irrespective of t(11;14) status. (bvsalud.org)

A rising chief in adipose-derived cell therapy and regenerative medicine, Tissue Genesis, Inc. is a high-development, clinical-stage company in Honolulu, Hawai'i. (kaushalenterprise.com)
Genesis Medical additionally affords Botox® injections, chemical peels, physique sculpting, pores, skin rejuvenation, dermal fillers, DNA testing, stem-cell therapy for Regenerative Medicine, and extra, aiming to make every patient feel confident and comfy in their very own pores and skin. (kaushalenterprise.com)
In addition to these laboratory-intensive courses, the core coursework also includes a quarterly Regenerative Medicine Seminar and a seminar-style Principles of Stem Cell Biology course. (ca.gov)
Through this coursework, students learn both the theoretical knowledge and practical skills that are important for stem cell research/regenerative medicine and gain important preparation for their internship projects. (ca.gov)
2014-Present Professor Liv Hospital (Ulus) Regenerative Medicine Stem Cell Production Center Istanbul- Turkey. (livhospital.com)

Differentiated airway epithelial cells can revert into stable and functional stem cells in vivo. (wikipedia.org)

Primary satellite cells can be harvested from muscle tissue to investigate or even use as potential therapeutic application. (sun.ac.za)
In conclusion, using clones that were distinctly different as assessed by the comparative index, this thesis illuminates that the two FGF family members investigated, act on cell cycle in different ways, thus would influence their utilization in experimental or therapeutic applications. (sun.ac.za)
Britain s House of Lords final approval of therapeutic human cloning and embryonic stem cells research has intensified the battle for ascendancy between adult and embryonic stem cells. (i-sis.org.uk)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Therapeutic cloning, which we discussed in Section 1, and which is one of the two sources of embryonic stem cells (ES), has brought to the center of the debate the issues of "humanness" and the legal status of an embryo: these are areas involving great controversy and discussion involving ethics, morality, and philosophy. (uofe.org)
The use of tissue-engineered grafts to repair urethral defects is considered to be an approach with therapeutic potential. (biomedcentral.com)
Recent studies have detected pervasive aberrant splicing in cancer cells, supporting its targeting for novel therapeutic strategies. (bvsalud.org)

Neither report cited a paper published last year in the journal Blood [4], where a group from the Stem Cell Institute, Department of Medicine, and Cancer Center, University of Minnesota Medical School, Minneapolis, reported the most comprehensive experiments proving that a single adult stem cell can differentiate into all cell types in culture. (i-sis.org.uk)
The cells could be made to differentiate into bone forming cells, cartilage forming cells, fat cells, skeletal muscle cells and endothelial cells. (i-sis.org.uk)
The French flag model represents how embryonic cells receive and respond to genetic information and subsequently differentiate into patterns. (asu.edu)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
5) Satellite cells proliferate , differentiate, and fuse with existing myofibers. (english-dictionary.help)
10) Stem cells are precursor cells that can proliferate , differentiate, and self-renew. (english-dictionary.help)
Some mesoangioblasts localize beneath the basal lamina and express c-met, whereas others differentiate into smooth muscle cells at the periphery of vessels and express α-smooth muscle actin. (illinois.edu)
Discussion & conclusion: Mesoangioblasts differentiate into multiple cell types damaged during the progression of severe muscle disease and protect fibers from damage. (illinois.edu)
Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. (biomedcentral.com)

In this study, we observed the overexpression of SUMO-speci﫿c protease 2 (Senp2) in adipose tissues during obesity. (deepdyve.com)
Excess or insuf﫿cient lipid storage in generation of the intermediate species of diacylglycerol (DAG) adipose tissues would impair nutrient homeostasis, which is and monoacylglycerol (MAG) (Young and Zechner, 2013). (deepdyve.com)
Although less fat stor- adqcKO pose lipid storage in adipocyte-speci﫿c Senp2 knockout mice fed age was shown in Senp2 adipose tissues, the increased with high-fat diets (HFD). (deepdyve.com)
Therefore, adipocyte-speci﫿c remain in adipose tissues. (deepdyve.com)
confirming that pecans modulate adipose tissues lipolysis and mitochondrial oxidative metabolism in the liver and skeletal muscle. (worldhealth.net)

Some in vivo transplantation studies have reported robust (35-50%) levels of transdifferentiation, which makes it unlikely that the results are due to cell fusion events. (i-sis.org.uk)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)
Detailed Description of Training Program Coursework: Our core coursework (i.e. courses taken by students from all three majors) is laboratory intensive, and includes Tissue Engineering, Cell Transplantation, Biomedical Imaging, and Molecular Techniques. (ca.gov)
cells for allogeneic transplantation without immunosuppression 6,153,428 .alpha. (europeanstrokenetwork.eu)
Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
2005 (October 1- December 15) Visiting Scientist Harvard Medical School, Joslin Diabetes Centre, Section of Islet Transplantation and Cell Biology, Boston/USA. (livhospital.com)

Progenitors are obtained by so-called direct reprogramming or directed differentiation and are also called induced somatic stem cells. (wikipedia.org)
This meant that the cells can change their differentiation pathway. (wikipedia.org)
In Drosophila imaginal discs, cells have to choose from a limited number of standard discrete differentiation states. (wikipedia.org)
The fact that transdetermination (change of the path of differentiation) often occurs for a group of cells rather than single cells shows that it is induced rather than part of maturation. (wikipedia.org)
Somatic cells are cells that have gone through the differentiation process and are not germ cells. (asu.edu)
Expression of SSEA-4 is down-regulated following differentiation of human EC, ES, and iPS cells. (stemcell.com)
In contrast, the differentiation of mouse EC, ES, or iPS cells may be accompanied by an increase in SSEA-4 expression. (stemcell.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Thereafter, dermomyotome-derived mpc clones rapidly expand while some progeny undergo terminal differentiation, leading to stochastic clonal drift within the mpc pool. (ox.ac.uk)
Here, using high-resolution timelapse imaging, we find that changes to cell geometry associated with dense tissue packing play a significant role in regulating differentiation rate in the zebrafish neural tube. (biologists.com)
Using simulations we show that interplay between progenitor density, cell shape and changes in differentiation rate could naturally result in negative-feedback control on progenitor cell number. (biologists.com)

To understand the role of autophagy in the pathogenesis of glial tumors in vivo, an established Drosophila melanogaster model of glioma was used based on overexpression in larval glial cells of an active human EGFR and of the PI3K homolog Pi3K92E /Dp110. (sdbonline.org)

The brain integrates metabolic signals from peripheral tissues such as the liver, pancreas, adipose tissue, gut and muscle. (nature.com)

The brain modulates various aspects of metabolism, such as food intake, energy expenditure, insulin secretion, hepatic glucose production and glucose/fatty acid metabolism in adipose tissue and skeletal muscle. (nature.com)
2010). Senp2 also regulates fatty acid metabolism in skeletal Downloaded from https://academic.oup.com/jmcb/article-abstract/10/3/258/4763638 by Ed 'DeepDyve' Gillespie user on 26 June 2018 Senp2 regulates adipose lipid storage by de-SUMOylation of Setdb1 j 259 muscle (Koo et al. (deepdyve.com)

The hybrid cells carrying four sets of chromosomes (instead of the usual two) behaved as stem cells when injected into mouse embryos. (i-sis.org.uk)
Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
Platt followed the paths of cells in developing mudpuppy embryos to see how embryonic cells migrated during the formation of the head. (asu.edu)
To determine the tissue distribution of GASP-1 mRNA, a 551-bp fragment of GASP-1 was amplified from first-strand cDNA produced from a variety of mouse tissues and staged embryos. (heightquest.com)
The first stem cells were removed from spare embryos at fertility clinics in 1998 and a human embryonic stem cell line was set up on a permanent basis. (uofe.org)

Carrel's ideas about cellular immortality convinced his many contemporaries that cells could be maintained indefinitely. (asu.edu)
The cellular precursors of skeletal muscle originate in somites that form along the dorsal side of the organism. (asu.edu)
11) This work tests the novel notion that cancer cells co-opt cellular pathways that govern metabolism in order to proliferate beyond a cell's normal means. (english-dictionary.help)
In conclusion, our data indicate that most mpcs in muscle tissue contribute to local growth and repair and suggest that cellular turnover is low in the absence of trauma. (ox.ac.uk)
These porous microstructures allow cell infiltration into the matrix and have fewer heterogeneous cellular proteins, thereby preventing complications, such as inflammatory reactions, fibrosis, calcification, and graft shrinkage. (biomedcentral.com)
Her research focuses on three areas: optimizing immune monitoring techniques by flow or mass cytometry, studying approaches to enhance the T-cell responses to human metastatic cancers, and optimization/manufacturing of cellular product for phase I clinical trials. (socalflow.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Created by Lewis Wolpert in the late 1960s, the model uses the French tricolor flag as visual representation to explain how embryonic cells can interpret genetic code to create the same pattern even when certain pieces of the embryo are removed. (asu.edu)
Such pathologies include genetic/metabolic diseases, erythroid tissue homeostatic disorders such as sickle cell anemia SCA, thalassemia, various anemias, degenerative bones, skeletal muscles, cartilage-forming tissue, and vascular mattress and some types of cancer, all considered as severe unmet medical needs. (kaushalenterprise.com)
To address questions of stem cell diversity during skeletal myogenesis, a Brainbow-like genetic cell lineage tracing method, dubbed Musclebow2, was derived by enhancer trapping in zebrafish. (ox.ac.uk)
as result, genetic or pharmacologic approaches that aim to modify CD38 surface-level prime MM cells to NAD+-lowering agents. (bvsalud.org)
A comprehensive gene expression analysis confirmed the reported deregulation of spliceosome machinery components in MM cells, compared to normal plasma cells from healthy donors, with its pharmacological and genetic modulation resulting in impaired growth and survival of MM cell lines and patient-derived malignant plasma cells. (bvsalud.org)

Among the many central goals of RM are the rigorous stem cell analysis, cell phenotype interconversion, and the event of innovative gene- and cell-based mostly therapies overall for the potential therapy/cure of degenerative disorders. (kaushalenterprise.com)
At birth, some affected newborns may have a minor skeletal phenotype, such as humpback, chest protrusion, and prominent forehead, as confirmed with radiography. (medscape.com)

Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)

Adult stem cells may be helpful in repairing eroded cartilage. (uofe.org)
GALNS deficiency induces the accumulation of glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) in multiple tissues, particularly bone, cartilage, heart valves, and cornea, whereas β-galactosidase deficiency induces the accumulation of only KS in those tissues. (medscape.com)

A germ layer is a group of cells in an embryo that interact with each other as the embryo develops and contribute to the formation of all organs and tissues. (asu.edu)
The formation of new cells, tissues, and organs enables animals to recover from day-to-day wear and tear, injury, and disease. (cshlpress.com)
Adult stem cells are immature cells found in bone marrow and other organs in adult human beings, as well as umbilical cords normally discarded at birth. (uofe.org)

Unlike FGF2, individual treatment with FGF6 was adequate to activate the quiescent PHMs and aid their re-entry into cell cycle with consistency in all three PHM clones by upregulating ki67 expression. (sun.ac.za)
This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
By directing AAV transduction through the regulated expression of its receptor, the approach provides precise control over which cells express the AAV-vectored transgene. (nature.com)
We hypothesized that AAV transgene expression could be targeted to specific tissues or cell types by selectively overexpressing AAVR. (nature.com)
The involvement of miRNAs in cancer research emerged from studies demonstrating their aberrant expression in neoplastic tissues. (genengnews.com)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
GASP-1 appears to be fairly widely expressed, with particularly high expression in skeletal muscle and heart. (heightquest.com)
All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
We show fasting's ability to increase CBIs' antitumour effects to depend on the reduction in circulating insulin, insulin-like growth factor-1 and leptin, which blunts the expression of enzymes from the cholesterol biosynthesis pathway and enhances cholesterol efflux from cancer cells. (bvsalud.org)
Here, we evaluated the expression of several spliceosome machinery components in multiple myeloma (MM) cells and the impact of splicing modulation on tumor cell growth and viability. (bvsalud.org)

In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852) and EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit (Catalog #18782). (stemcell.com)

Although most individuals with Morquio syndrome appear normal at birth, skeletal abnormalities often develop within the first year of life. (medscape.com)

Discovery of iron-sulfur cofactors will lead to breakthroughs in several research areas involving DNA repair, ribosomal biogenesis, mRNA translation, intermediary metabolism, and regulation of the growth and energy-sensing pathways that are critical for determining the fates of many cell types. (nih.gov)
IREs are RNA stem-loops found in the 5′ end of ferritin mRNA and the 3′ end of transferrin receptor mRNA. (nih.gov)

binding of the RM4-5 antibody can be blocked by the clone GK1.5 antibody. (stemcell.com)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 40x lens. (ptglab.com)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 10x lens. (ptglab.com)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27867-1-AP (Titin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptglab.com)
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptglab.com)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. (ptglab.com)

Moreover, they can make this transition even in the absence of noticeable injuries and are capable of replenishing entire gastric units, in essence serving as quiescent "reserve" stem cells. (wikipedia.org)
Secondly, the quiescent state is an integral part of stem cell regulation, therefore choosing the right protocol for inducing quiescence is important. (sun.ac.za)

Under physiological conditions, the main- PPARγ mutation has been shown to link to familial partial lipody- tenance of normal adipose tissue mass is mainly the result of a strophy, a clinical disorder characterized by the loss of adipose balance of lipid storage and lipolysis (Bouchard et al. (deepdyve.com)

They are classified as either totipotent (iTC), pluripotent (iPSC) or progenitor (multipotent - iMSC, also called an induced multipotent progenitor cell - iMPC) or unipotent - (iUSC) according to their developmental potential and degree of dedifferentiation. (wikipedia.org)

The main research theme of her lab is neural stem cells, and the molecular pathophysiology of Huntington's Disease . (eurostemcell.org)

4) Upon muscle injury, these cells undergo mitosis, proliferate , form syncytium and ultimately form new skeletal myocytes. (english-dictionary.help)

In the second report [3], mouse bone marrow cells marked with green fluorescent protein were found to fuse with ES cells in culture in the presence of the cytokine, interleukin-3, which is known to encourage cell fusion. (i-sis.org.uk)
These researchers pointed out that the spontaneous fusion rate (without interleukin-3) was extremely low, between 2-11 per million bone marrow cells, and is unlikely to account for all the findings with adult stem cells. (i-sis.org.uk)
Myostatin RNA is produced nearly exclusively in skeletal muscle[so muscular exercise may help bone growth by inhibiting myostatin]. (heightquest.com)
Lupus patients treated with stem cells from their own bone marrow have exceeded expectation. (uofe.org)
Cardiac function is believed to be improved in heart arteries that have been injected with adult bone marrow stem cells. (uofe.org)
In some studies leukemia patients treated with stem cells from bone marrow and umbilical cord blood became free of disease (as noted above). (uofe.org)
Prophylaxis against bone loss should be given to all transplant recipients, regardless of baseline skeletal reserve. (medscape.com)
Regular weight-bearing and muscle-strengthening exercises are recommended to improve bone strength and reduce the risk of fracture. (medscape.com)
Skeletal phenotyping, such as measuring bone mineral density (BMD) and histomorphometry analysis with bone biopsy, has provided an explanation, in part, of the elevated fracture risk. (medscape.com)

Myogenesis is the formation of muscle that occurs throughout an animal's development, from embryo to the end of life. (asu.edu)

With her research, Platt challenged then current theories about germ layers, the types of cells in an early embryo that develop into adult cells. (asu.edu)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
You can see how many people began to react and to wonder if this would result in human cloning factories and embryo farms to cultivate human spare parts. (uofe.org)

The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)
Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. (deepdyve.com)
Professor Elena Cattaneo is Director of the Laboratory of Stem Cell Biology and Pharmacology of Neurodegenerative Diseases at the Department of Pharmacological Sciences, as well as a co-founder and first appointed Director of UniStem , the Centre for Stem Cell Research of the University of Milano. (eurostemcell.org)

Clonal behaviour of myogenic precursor cells throughout the vertebrate lifespan. (ox.ac.uk)

His claim was based on chick-heart tissue cultures in his laboratory that seemed to be able to proliferate forever. (asu.edu)
In the 1960s, however, Carrel's thesis about cell immortality was put into question by the discovery that human diploid cells can only proliferate for a finite period. (asu.edu)
1) If cloning by fission is viewed more generally as a form of tissue modeling seen in all metazoans, in which some cells proliferate and others die, knowledge from other organisms can suggest candidate genes. (english-dictionary.help)
2) At the time, the notion that we could proliferate stem cells from an adult kidney, or any other organ for that matter, was hardly the conventional wisdom that it is today. (english-dictionary.help)
3) If you have any cells with estrogen receptors and you put more estrogen into the body, you're going to stimulate those cells to proliferate and divide. (english-dictionary.help)
13) In later life we are not supposed to continue to proliferate tissue at a rapid rate, grow, and accumulate mass, but rather to mature. (english-dictionary.help)
14) The cells remaining in the ruptured follicle proliferate rapidly and form the corpus luteum. (english-dictionary.help)
It is shown that, after initial formation of the primary myotome, at least 15 muscle precursor cells (mpcs) seed each somite, where they proliferate but contribute little to muscle growth prior to hatching. (ox.ac.uk)
Mesoangioblasts proliferate in vivo, incorporate into muscle fibers, form new fibers, and promote synthesis of clystrophin and utrophin. (illinois.edu)

Other key factors would involve electrically stimulating and mechanically stretching the muscles to exercise them, helping them mature properly, and perhaps growing other cells alongside the satellite cells to provide necessary molecular cues. (livescience.com)
Although many molecular regulators have been established, how these connect to physical and geometrical aspects of tissue architecture is poorly understood. (biologists.com)
Nonetheless, it appears that in order to cure aging we will need to target multiple types of cells and possibly address different types of molecular damage and malfunction. (senescence.info)

Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expresed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. (lu.se)

The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
Materials & methods: We have isolated wild-type mesoangioblasts from aorta and tested their effectiveness in alleviating severe muscle disease in the dystrophin/utrophin knockout (mdx/utrn -l- ) mouse model for Duchenne muscular dystrophy. (illinois.edu)

For instance, multilineage-differentiating stress-enduring (Muse) cells are stress-tolerant adult human stem cells that can self-renew. (wikipedia.org)
hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
In Warren Ellis and Darick Robertson's epic sci-fi satire "Transmetropolitan," supermarkets and fast food joints sell dolphin, manatee, whale, baby seal, monkey and reindeer, while the Long Pig franchise sells "cloned human meat at prices you like. (livescience.com)
In principle, we could harvest the meat progenitor cells from fresh human cadavers and grow meat from them," Post said. (livescience.com)
Injecting stem cells into a human patient entails the same problem as other types of transplants: rejection of the foreign cells by the person's immune system. (uofe.org)

In skeletal muscle, these are primary satellite cells. (sun.ac.za)
Satellite cells exist in quiescence in the muscle tissue and only become activated following an insult. (sun.ac.za)
To grow meat in labs from satellite cells, the researchers suggested current tissue-engineering techniques, where stem cells are often embedded in synthetic three-dimensional biodegradable matrixes that can present the chemical and physical environments that cells need to develop properly. (livescience.com)
Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
Another stimulus that activates satellite cells is stretching of the muscle. (arvojournals.org)
7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)

Some types of mature, specialized adult cells can naturally revert to stem cells. (wikipedia.org)
The finding that adult cells can fuse with ES cells is therefore irrelevant to the debate, and the fact that it was used in an attempt to discredit adult stem cells is itself revealing, particularly in view of other recent findings on ES cells. (i-sis.org.uk)
They found that the spontaneous mutation frequency in ES cells is 100-fold lower than that in mouse embryonic fibroblasts (a somatic cell line), which is similar to adult cells in vivo . (i-sis.org.uk)

However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. (dattanibookagency.in)
We still do not know whether aging derives from changes in a specific organ or if there are intrinsic age-related changes in each tissue that are independent of one another, as discussed elsewhere . (senescence.info)

This was confirmed by examining the cells chromosomes and identifying the distinctive chromosomes from both mouse lines. (i-sis.org.uk)
The fused cells were found to have 4 to six sets of chromosomes by DNA analysis, and they behaved as ES cells when tested. (i-sis.org.uk)

superfamily, is a potent and specific negative regulator of skeletal muscle mass. (heightquest.com)

In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
IRP2 also binds to IREs in iron-depleted cells but, unlike IRP1, in iron-replete cells it is selectively ubiquitinated and then degraded by the proteasome. (nih.gov)

Here we generate a robust system for in vivo transgene delivery by combining the versatility of AAV-mediated transgene delivery with the specificity of cell-type-specific Cre-mediated recombination. (nature.com)

Project opportunities will span discovery-based research in academic labs to device engineering at for-profit companies, and encompass most of the major physiological systems- cardiovascular, endocrine, musculo-skeletal, hepatic, renal, skin and immune. (ca.gov)

Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
We have cloned mouse GASP-1 and shown that it inhibits the biological activity of mature myostatin , but not activin. (heightquest.com)

These neurons project to other hypothalamic nuclei and to the nucleus of the solitary tract in the brain stem to control multiple aspects of the homeostatic regulation of energy balance. (nature.com)

20 or so clones of every genotype were examined, where 15 samples were given freezin Seafood epidermis symbolizes early vertebrate mucosal area, sharing traits with mucosal floors such as that relating to the actual intestine. (pf-8380inhibitor.com)
Since germ therapy is too late for everyone alive, we have to correct the genotype of a significant number of our somatic cells or replace them by new ones. (senescence.info)

Anatomy63

Organisms17

Diseases18

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes55

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture1

Information Science3

Health Care1

Regeneration15

Mesenchymal stem1

Research and regenerative medicine2

Pluripotent cells1

Hematopoietic2

Proliferation11

Vitro9

Regenerative medicine5

Epithelial cells1

Therapeutic11

Differentiate9

Adipose tissues5

Transplantation11

Differentiation21

Glial cells1

Liver1

Fatty acid metab2

Embryos5

Cellular7

Genetic5

Phenotype2

Somatic-cell nuc1

Cartilage2

Organs3

Expression11

Myofibers6

Isolation1

Abnormalities1

MRNA2

Antibody6

Quiescent2

Lipolysis1

Progenitor cell1

Neural Stem1

Myocytes1

Bone9

Myogenesis1

Embryo3

Biology3

Myogenic1

Proliferate9

Molecular3

Lung1

Methods2

Human5

Satellite cells6

Adult cells3

Organ3

Chromosomes2

Regulator1

Selectively2

Vivo1

Renal1

Mature2

Homeostatic1

Genotype2