• Reprogramming of human fibroblasts into multipotent cells with a single ECM proteoglycan, fibromodulin. (ca.gov)
  • We report here a technique using a single molecule, recombinant human fibromodulin protein (FMOD), to reprogram human fibroblasts into multipotent cells. (ca.gov)
  • They found that the spontaneous mutation frequency in ES cells is 100-fold lower than that in mouse embryonic fibroblasts (a somatic cell line), which is similar to adult cells in vivo . (i-sis.org.uk)
  • Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
  • MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)
  • We found that INK4b and INK4a , but not ARF , are upregulated following the differentiation of haematopoietic progenitor cells, in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. (biomedcentral.com)
  • Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle. (advancedbiomatrix.com)
  • Skeletal muscle tissue, which makes up 50 percent of the body, is easily accessible and biopsies of muscle are relatively harmless to the donor, so we think it may be an alternative source of neural-like cells that potentially could be used to treat brain or spinal cord injury, neurodegenerative disorders, brain tumors and other diseases, although more studies are needed. (wakehealth.edu)
  • Right now, patients with glioblastomas or other brain tumors have very poor outcomes and relatively few treatment options," said Alexander Birbrair, a doctoral student in Delbono's lab and first author of these studies. (wakehealth.edu)
  • Because our cells survived and migrated in the brain, we may be able to use them as drug-delivery vehicles in the future, not only for brain tumors but also for other central nervous system diseases. (wakehealth.edu)
  • Study in Cell led by Stony Brook researcher provides unique analysis in a glioma model STONY BROOK, NY, August 15, 2023 - Gliomas are incurable brain tumors. (stonybrook.edu)
  • [ 9 ] They may be derived from mesenchymal stem cells. (medscape.com)
  • However, it increases proliferation rates in some cell types (e.g. mesenchymal stem cells, osteosarcoma cells, HUVECs) which puts extra demands on nutrient supply and waste removal [1,2,3] . (reprocell.com)
  • In vitro expansion of mesenchymal stem cells using 3-D matrix derived from cardiac fibroblast. (reprocell.com)
  • But if there is no alteration in the repair process, Mesenchymal cells kickstart proliferative process and begin to repair and restructure the affected tissues starting from the base. (issca.us)
  • Just as stated above, during the proliferative phase of wound healing, Mesenchymal cells are the key role players. (issca.us)
  • Their structure includes a Mesenchymal stem cell (MSCs), multi potent in nature and offer supportive, therapeutic and trophic functions. (issca.us)
  • Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • The study , published May 11 in the peer-reviewed journal Cell Stem Cell, identified various cell types present in skeletal muscle tissues, from early embryonic development all the way to adulthood. (scienceblog.com)
  • Leveraging the power of this technology and the Plath lab's bioinformatics expertise, the group identified the genetic signatures of various cell types from human tissues and pluripotent stem cells. (scienceblog.com)
  • Notably, FReP cells regenerate muscle and bone tissues but do not generate teratomas in vivo. (ca.gov)
  • The age-associated progressive degeneration of many tissues is thought to be driven by the functional decline and gradual attrition of adult stem cells. (helmholtz-muenchen.de)
  • They may instead be fusing with existing cells, creating genetically mixed-up tissues with unknown health effects" [1]. (i-sis.org.uk)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
  • Physiological glucose concentration in tissues is however significantly lower compared to that used in most cell culture studies and glucose concentration is even lower in tumor tissues. (grantome.com)
  • MSCs also possess subpopulations that are stem-like nature commonly referred to as "side population" (SP) they have been found out to be enriched in over 1000-fold of progenitor cells and multipotent stem cells and as well exist in tissues and tumors. (issca.us)
  • However, these adult stem cells have more restricted potential - they are specialized to replenish, rejuvenate, and repair the tissues in which they reside. (pas.va)
  • They are particularly active in tissues and organs in which the lifespan of the differentiated cells is short, like blood, skin and the inner cell layer covering the intestinal cavity, as well as in the repair of skeletal muscle after exercise of injury. (pas.va)
  • Significant advances have been made in isolating, culturing and reintroducing adult stem cells into tissues. (pas.va)
  • With these environmental cues in mind, biologists have developed more physiologically accurate methods for culturing cells and tissues, including new media formulations and co-culture techniques. (mimetas.com)
  • PCB methyl sulphones and related compounds: Identification of target cells and tissues in different species. (cdc.gov)
  • An interdisciplinary team of researchers at the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at UCLA has developed a first-of-its-kind roadmap of how human skeletal muscle develops, including the formation of muscle stem cells. (scienceblog.com)
  • The roadmap is critical for researchers who aim to develop muscle stem cells in the lab that can be used in regenerative cell therapies for devastating muscle diseases, including muscular dystrophies, and sarcopenia, the age-related loss of muscle mass and strength. (scienceblog.com)
  • The potential application of stem cells in regenerative medicine is increasingly promising especially in defects that are difficult to be self -regenerate. (ca.gov)
  • The principle challenge today is to produce enough regenerative cells through a simple, consistent process that bypasses ethical and immunological barriers and avoids genome alteration for in vivo skeletal muscle and bone construction without causing tumor formation. (ca.gov)
  • and [3] therapeutic innovation - FMOD reprogrammed (FReP) multipotent cells can be easily differentiated into skeletal myocytes and osteoblasts in vitro and in vivo, respectively, and will have significant clinical value in regenerative medicine to establish skeletal muscle and bone by autologous cells. (ca.gov)
  • Pluripotent and/or multipotent stem cell-based therapeutics are a vital component of tissue engineering and regenerative medicine. (ca.gov)
  • Skeletal muscle mass and function decline with age (sarcopenia), linked to progressive reduction in regenerative capacity of skeletal muscle stem cells (satellite cells). (helmholtz-muenchen.de)
  • In fact, many of the regenerative properties of muscle progenitor cells can be credited to the vesicles, Xuan says. (augusta.edu)
  • Indeed, exposing aggregated P19 cells to dimethyl sulfoxide (DMSO) induces differentiation into cardiac and skeletal muscle. (wikipedia.org)
  • By aggregating the cells into an embryonic body, EC cells can also process differentiation. (wikipedia.org)
  • In P19 cells, addition of non-toxic concentrations of drugs to aggregated embryoid body cells can induce differentiation of P19 cells into specific cell lines depending on the added drug. (wikipedia.org)
  • Because the concentration of retinoic acid or DMSO is nontoxic to the cells, the drug-specific differentiation is due to induction of cells not selection. (wikipedia.org)
  • Mutants of P19 cells have been generated to investigate the mechanism of drug-specific differentiation. (wikipedia.org)
  • The research group was able to show in human skeletal muscle cell lines and in a mouse model that Wnt7a treatment counteracted muscle wasting and improved muscle stem cell differentiation - two major symptoms of cachexia in cancer patients. (leibniz-gemeinschaft.de)
  • Remarkably, in a fashion reminiscent of quiescent stem cells, the slow replicative phenotype of undifferentiated FReP cells reverses after differentiation induction, with differentiating FReP cells proliferating faster and expressing less p15(Ink4B) and p21(WAF1/Cip1) than differentiating iPS cells. (ca.gov)
  • DMH-1 induces intestinal differentiation in human intestinal organoids (hIOs) derived from human pluripotent stems cells (hPSCs). (tocris.com)
  • 1146.Regulation of differentiation potentialof humanmesenchymal stem cells by intracytoplasmic delivery ofcoactivator-associatedarginine methyltransferase 1 protein usingcell-penetrating peptide.Jo J, Song H, Park SG, Lee SH, KO JJ,Park JH, Jeong J, Cheon YP,Lee DR. (miraewa.com)
  • In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
  • During progenitor cell differentiation and ageing, PcG silencer EZH2 attenuates, causing loss of PRC binding and transcriptional activation of INK4b and INK4a . (biomedcentral.com)
  • Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
  • Development and homeostasis require the coordinate regulation of cell proliferation and differentiation. (biomedcentral.com)
  • These newly available materials led to compelling evidence that the physical cellular environment is far from inert, but acts as a biological and mechanical signal to regulate cell-type specific function, differentiation, and morphology 1 . (mimetas.com)
  • Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. (advancedbiomatrix.com)
  • Isolation, characterization, and differentiation of human multipotent dermal stem cells. (advancedbiomatrix.com)
  • Focusing on muscle progenitor cells, which contribute to muscle formation before birth, and muscle stem cells, which contribute to muscle formation after birth and to regeneration from injury throughout life, the group mapped out how the cells' gene networks - which genes are active and inactive - change as the cells mature. (scienceblog.com)
  • The group found that pluripotent stem cell-derived muscle cells produced by all the methods they tried resembled muscle progenitor cells at an early developmental state and did not align to adult muscle stem cells. (scienceblog.com)
  • Consequently, neural stem and progenitor cells have usually been studied retrospectively, based on their ability to form colonies in laboratory cell cultures. (elifesciences.org)
  • Gcells are conditioned to work with an enriched suspension of progenitor cells that can efficiently aid tissue repair process. (issca.us)
  • As the name implies, induced pluripotent stem cells can become any type of cell in our body, and scientists have evidence that when they prompt them to become muscle progenitor cells they can help restore the sometimes debilitating muscle loss that happens with age. (augusta.edu)
  • She has evidence that one of the most efficient and effective ways to do that is by using a small manmade molecule called givinostat to coax the induced pluripotent stem cells, or iPSCs, to become these muscle, or myogenic, progenitor cells. (augusta.edu)
  • Xuan is principal investigator on a $1.6 million grant (1RO1AG070145-01) from the National Institutes of Health to further explore the benefit of directly administering these myogenic progenitor cells or the extracellular vesicles they produce. (augusta.edu)
  • Givinostat, which is in the final phase of clinical trials for muscular dystrophy, is a manmade small molecule that inhibits HDACs, enzymes that help turn genes off, and can steer iPSCs to become muscle progenitor cells and muscle-specific extracellular vesicles. (augusta.edu)
  • She suspects that in older individuals who have an actual muscle injury in the face of sarcopenia, giving the muscle progenitor cells themselves may be a better option, because of the individual's impaired ability to generate new muscle cells that may be needed for a full repair. (augusta.edu)
  • When we are younger, skeletal muscle stem cells called satellite cells hovering in the vicinity of our muscles, produce most of the muscle progenitor cells and extracellular vesicles in response to exercise or injury. (augusta.edu)
  • Stony Brook, NY, June 13, 2023 - Benjamin Martin, PhD, Associate Professor in the Department of Biochemistry and Cell Biology at Stony Brook University, and member of the Stony Brook University Cancer Center, has received a $2 million grant from the National Institutes of Health's National Institute of General Medical Sciences ( NIGMS ) to conduct research to understand the molecular and cell biology of neuromesodermal progenitors. (stonybrook.edu)
  • Desmoid tumors are cytologically bland fibrous neoplasms originating from the musculoaponeurotic structures throughout the body. (medscape.com)
  • Desmoid tumors often appear as infiltrative, usually well-differentiated, firm overgrowths of fibrous tissue, and they are locally aggressive. (medscape.com)
  • Although desmoid tumors can arise in any skeletal muscle, they most commonly develop in the anterior abdominal wall and shoulder girdle. (medscape.com)
  • [ 4 ] . Implant-associated breast desmoid tumors may occur. (medscape.com)
  • Immunostaining with vimentin, alpha smooth muscle actin, muscle actin, and desmin are helpful in distinguishing the tumors in the differential diagnosis of desmoid tumors. (medscape.com)
  • Primary surgery with negative surgical margins is the most successful primary treatment modality for desmoid tumors. (medscape.com)
  • [ 7 ] Since desmoid tumors do not metastasize, many approaches are nonoperative in nature. (medscape.com)
  • [ 8 ] Since desmoid tumors may stabilize and regress, active observation is an option. (medscape.com)
  • Although desmoid tumors most commonly arise from the rectus abdominis muscle in postpartum women and in scars due to abdominal surgery, they may arise in any skeletal muscle. (medscape.com)
  • Desmoid tumors tend to infiltrate adjacent muscle bundles, frequently entrapping them and causing their degeneration. (medscape.com)
  • The myofibroblast is the cell considered to be responsible for the development of desmoid tumors (see Procedures). (medscape.com)
  • Additionally, evidence also exists for a genetic predisposition to desmoid tumors in FAP, independent of the APC mutation. (medscape.com)
  • Desmoid tumors occur at a rate of 10-15% in patients with FAP, an autosomal inherited disease caused by germline mutations in the APC gene. (medscape.com)
  • [ 11 ] Desmoid tumors show biallelic APC mutation, with one change usually occurring distal to the second beta-catenin binding/degradation repeat of the gene (3' to codon 1399). (medscape.com)
  • However, unknown genetic factors independent of APC may be important in the susceptibility to desmoid tumors in patients with FAP. (medscape.com)
  • In desmoid tumors, one of the two mutations usually occurs distal to the second beta-catenin binding/degradation repeat of the gene (3' to codon 1399). (medscape.com)
  • In desmoid tumors, 1 of the 2 mutations usually occurs distal to the second beta-catenin binding/degradation repeat of the gene (3' to codon 1399). (medscape.com)
  • Independent predictors of increased desmoid risk in one study were said to be (1) germline mutation distal to codon 1399, (2) any family history of gastrointestinal disease, and (3) a strong family history of desmoid tumors. (medscape.com)
  • Desmoid tumors are associated with a biallelic APC mutation in the affected tissue. (medscape.com)
  • In genetically normal individuals, with normal germline genes, this necessitates a rare combination of events, such that at least 2 somatic mutations must occur in both alleles of a single tumor suppressor gene, in this case the APC gene. (medscape.com)
  • But, although the mutation frequency of genes was much lower in ES cells, mutant ES cells accumulated with time in culture. (i-sis.org.uk)
  • At this physiological range of glucose, cancer cells maintain high glycolytic rate and genes that regulate cancer cell glycolysis may resist biguanide action by upregulating compensatory glycolysis when mitochondria is inhibited. (grantome.com)
  • Therefore, identification and inhibition of such genes may enhance cancer cell liability towards biguanides. (grantome.com)
  • We will test the genes that confer resistance to metformin in glioma stem cells. (grantome.com)
  • Appearance profiles from the cells in the G0 stage uncovered that they portrayed a couple of genes linked to cell fat burning capacity, inflammatory response, tumor and epigenetics suppression. (e-core.org)
  • Characteristics of Clonal Hematopoiesis (CH) Mutations - a) We identified somatic mutations in known clonal hematopoiesis of indeterminate potential (CHIP) driver genes using peripheral blood mononuclear cells isolated from 14 astronauts who flew short space Shuttle missions lasting a median of 12 days between 1998-2001. (spaceref.com)
  • The most frequent mutations occurred in TP53, a gene that produces a tumor-suppressing protein, and DNMT3A, one of the most frequently mutated genes in acute myeloid leukemia. (spaceref.com)
  • However, the frequency of the somatic mutations in the genes that the researchers assessed was less than two percent, the technical threshold for somatic mutations in hematopoietic stem cells to be considered clonal hematopoiesis of indeterminate potential (CHIP). (spaceref.com)
  • In each of these mice we have inactivated the genes that encode for synaptic signalling molecules, such as agrin and laminins, or molecules that are needed to cluster ion channels in the postsynaptic membranes of muscles and/or neurones, such as rapsyn and gephyrin. (edu.au)
  • This project in the first part is simply a promoter and 3' UTR analysis of some 30 genes that appear at the same time in the postsynaptic region of skeletal muscle. (edu.au)
  • Within the blood system, anemia of ageing is characterized by peripheral blood cytopenias, bone marrow aplasia and decreased engraftment capacity upon transplantation, indicating impaired hematopoietic stem cells function. (helmholtz-muenchen.de)
  • They discovered DNA mutations, known as somatic mutations, in the blood-forming system (hematopoietic stem cells) in all 14 astronauts studied. (spaceref.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Furthermore, muscle regeneration is affected during cancer cachexia due to impaired muscle stem cell function. (leibniz-gemeinschaft.de)
  • Our results show that Wnt7a has a dual function in skeletal muscle: it activates an important signaling pathway enhancing muscle mass and drives muscle stem cell expansion in skeletal muscle, which is necessary for regeneration", says Dr. Manuel Schmidt, postdoc in the research group. (leibniz-gemeinschaft.de)
  • Wnt7a leads to an increase in the stem cell population in the muscle, which supports muscle regeneration after surgery. (leibniz-gemeinschaft.de)
  • Thus proved that dental pulp holds potentialities of therapeutic strength and a rich source of progenitor/autologous cells that can be used to aid healing processes even applicable to regeneration of craniofacial bones. (issca.us)
  • In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
  • 20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
  • Additionally, we are interested in mechanisms responsible for skeletal muscle recovery and regeneration after injury. (uncg.edu)
  • More specifically, my laboratory investigates the role inflammation plays in muscle atrophy, as well as during recovery and regeneration after injury. (uncg.edu)
  • Aged skeletal muscle displays increased fibrosis and impaired regeneration. (uncg.edu)
  • In aged skeletal muscle, impairments in regrowth and regeneration may be explained by a decreased responsiveness of muscle precursor cells (MPCs) to environmental cues such as growth factors. (uncg.edu)
  • These cells make actual muscle cells as well as the extracellular vesicles - little nearby containers packed with muscle-specific cargo, like microRNAs - that aid muscle repair and regeneration. (augusta.edu)
  • Xuan already has identified some of the cargo, including mRNAs that activate cell signaling pathways important to muscle regeneration. (augusta.edu)
  • The organs with SP include the lung, liver, brain, mammary gland and in skeletal muscles. (issca.us)
  • 1998. Induction of mammary tumors in rats by single- dose gamma irradiation at different ages. (cdc.gov)
  • In addition, growing evidence has indicated that LKB1 functions as a tumor suppressor gene. (omicsdi.org)
  • This process is an example of the Knudsen "two hit" hypothesis, in which a tumor suppressor gene, such as APC , must be biallelically mutated in order for a specific type of tumor to occur. (medscape.com)
  • Researchers in Pyle's lab and others around the world already have the capacity to generate skeletal muscle cells from human pluripotent stem cells - cells that have the ability to self-renew and to develop into any cell type in the body. (scienceblog.com)
  • To create this resource, the group gathered highly specific data about two different groups of skeletal muscle cells: those from the human body, ranging from the fifth week of embryonic development to middle age, and those derived from human pluripotent stem cells the researchers generated in the lab. (scienceblog.com)
  • In addition to pinning down the true maturity of the lab-produced cells, this analysis also provided details about the other cell types present in skeletal muscle tissue across development and in populations derived from human pluripotent stem cells. (scienceblog.com)
  • Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M . Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302. (chinagene.cn)
  • Promotes neurogenesis in human induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. (tocris.com)
  • Induced pluripotent stem cells (iPSC) can now be generated from skin or blood of mice or humans by overexpressing four key transcription factors. (pas.va)
  • Chipman P.H., Toma J.S. and Rafuse V.F. Â Generation of motor neurons from pluripotent stem cells. (amap.ca)
  • It causes various types of benign or malignant tumors that involve central or peripheral nerves and often causes pigmented skin macules and sometimes other manifestations. (msdmanuals.com)
  • Benign tumors can be removed surgically, and malignant tumors (which are less common) can be treated with radiation therapy or chemotherapy. (msdmanuals.com)
  • It causes various types of benign or malignant tumors that involve central or peripheral. (msdmanuals.com)
  • Glioblastoma multiforme (GBM) is an incurable brain tumor with a median survival of 15 months and new therapy is badly needed. (grantome.com)
  • Studies have shown that a certain concentration of RA can induce P19 cells to differentiate into neuronal cells, including neurons and glial cells, whereas 0.5% - 1% DMSO led P19 cells to differentiate into cardiac or skeletal muscle cells. (wikipedia.org)
  • Using doses between 1 μM to 3 μM of RA can generate neurons as the most abundant cell type. (wikipedia.org)
  • Other than into neurons and astrocytes, P19 cells can also differentiate to oligodendrocytes, which can be detected using the specific markers, myelin-associated glycoprotein and 2',3'-Cyclic-nucleotide 3'-phosphodiesterase. (wikipedia.org)
  • Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
  • In addition, the Wake Forest Baptist team is now conducting research to determine if these neural-like cells also have the capability to become functioning neurons in the central nervous system. (wakehealth.edu)
  • Neurobiologist Dr. Vic Rafuse is making great strides in his work to develop new strategies for restoring crucial motor functions lost in ALS, while helping to re-shape the understanding of motor degenerative mechanisms in ALS. From his earlier work to engineer stem cells into motor neurons, he has evolved a new research direction that's using light to bypass the nervous system and stimulate skeletal muscles directly. (amap.ca)
  • Dr. Rafuse is well known for his past work to engineer adult human stem cells into functional motor neurons, for use in drug-sreening models and potential future therapies for ALS. (amap.ca)
  • He and his team were also able to induce the axons of motor neurons to make appropriate nerve-to-muscle connections after a peripheral nerve injury. (amap.ca)
  • Magown P., Rafuse V.F ., and Brownstone R.M. Â Microcircuit formation following transplantation of mouse embryonic stem cell-derived neurons into peripheral nerve. (amap.ca)
  • Â Presynaptic NCAM is required for motor neurons to functionally expand their peripheral field of innervation in partially denervated muscles. (amap.ca)
  • Martin and colleagues observe zebrafish to demonstrate how differentiated neurons and muscle expand as embryos grow. (stonybrook.edu)
  • This image captured by the Martin lab shows three different time points of a developing zebrafish to demonstrate how differentiated neurons and muscle expand as the embryos grow. (stonybrook.edu)
  • The image shows the zebrafish reporter transgenes that label skeletal muscle (magenta color) and neurons (green color). (stonybrook.edu)
  • 1987. The influence of skeletal muscle on the electrical excitability of dorsal root ganglion neurons in culture. (cdc.gov)
  • Somatic mutations are mutations that occur after a person is conceived and in cells other than sperm or egg cells, meaning they cannot be passed on to offspring. (spaceref.com)
  • The mutations identified in this study were characterized by the overrepresentation of blood cells derived from a single clone, a process called clonal hematopoiesis (CH). Such mutations are frequently caused by environmental factors, such as exposure to ultraviolet radiation or certain chemicals, and may be a result of cancer chemo- or radiotherapy. (spaceref.com)
  • This map identifies the precise gene networks present in muscle progenitor and stem cells across development, which is essential to developing methods to generate these cells in a dish to treat muscle disorders. (scienceblog.com)
  • This technology enables researchers to identify the gene networks present in a single cell and can process thousands of cells at the same time. (scienceblog.com)
  • They next developed computational methods to focus on muscle progenitor and stem cells and mapped out their gene networks associated with every developmental stage. (scienceblog.com)
  • Moreover, signaling pathways related to neurogenesis and myogenesis were also investigated by studying gene expression or generating mutants of P19 cells. (wikipedia.org)
  • Overall, single protein, FMOD-based, cell reprograming bypasses the risks of mutation, gene instability, and malignancy associated with genetically-modified iPS cells, and provides an alternative strategy for engineering patient-specific multipotent cells for basic research and therapeutic application. (ca.gov)
  • The researchers discovered that the protocells were able to produce energy-rich molecules (ATP) via glycolysis and synthesize RNA and proteins by in vitro gene expression, indicating that the inherited bacterial components remained active in the synthetic cells. (nanowerk.com)
  • As a step towards the construction of a synthetic/living cell entity, the researchers implanted living bacteria into the protocells to generate self-sustainable ATP production and long-term energization for glycolysis, gene expression and cytoskeletal assembly. (nanowerk.com)
  • This usually results from a spontaneous somatic mutation in the unaffected APC gene of a single cell in a patient with the FAP syndrome. (medscape.com)
  • In FAP syndrome patients, one APC germline gene is already mutated in every cell in the body (barring a rare reverse somatic mutation in some cells), and, therefore, only one new somatic mutation is required in the opposite APC gene for the tumor to develop. (medscape.com)
  • Genomiccloning of the Hsc 71 gene in the hermaphroditic teleostRivulusmarmoratus and analysis of its expression in skeletalmuscle: identificationof a novel muscle-preferred regulatoryelement. (miraewa.com)
  • This will involve looking at mice we are making where we the gene for TGFbeta is knockout out in adult muscle and/or in the adult spinal cord (a tissue inducible knockout out of TGFbeta 2). (edu.au)
  • Like virally-induced pluripotent stem (iPS) cells, FMOD reprogrammed (FReP) cells express pluripotency markers, form embryoid bodies (EBs), and differentiate into ectoderm, mesoderm, and endoderm derivatives in vitro. (ca.gov)
  • Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
  • term pre-GEPCOT cells (based on an acronym of the markers used to isolate the cells), were long-lived and quiescent, but they lacked the ability to form colonies in culture. (elifesciences.org)
  • uncovered that marker was helpful for discovering the quiescent cell inhabitants in skeletal muscle tissue using the markers of muscle tissue stem cells. (e-core.org)
  • To include biochemical and/or immunological markers which are specific for a tumour site (Table 2). (who.int)
  • The cells could be made to differentiate into bone forming cells, cartilage forming cells, fat cells, skeletal muscle cells and endothelial cells. (i-sis.org.uk)
  • In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
  • Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
  • VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
  • Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
  • Vascular endothelial growth factor (VEGF) is important for the maintenance of the skeletal muscle capillaries. (uncg.edu)
  • Recently, we observed that muscle capillarization, vascular endothelial growth factor (VEGF) protein, and the VEGF mRNA response to acute exercise were lower in aged compared with young women. (uncg.edu)
  • Since FMOD reprogramming clearly involves neither oncogenes nor genome integrative approaches and FReP cells do not generate teratoma in vivo, FMOD reprogramming fulfills the requirements of the FDA to produce safe multipotent cells without known undesirable side effects. (ca.gov)
  • Some in vivo transplantation studies have reported robust (35-50%) levels of transdifferentiation, which makes it unlikely that the results are due to cell fusion events. (i-sis.org.uk)
  • This discovery was in an in vitro and in vivo experiment when Dental Pulp Stem Cells (DPSCs) showed capability to differentiate into osteoblasts and built a woven bone by forming an Extracellular Matrix (ECM) secreted by the osteoblasts. (issca.us)
  • The experiment drew results on the both the quality and quantity of the matrix formed by the DPSCs in the in vivo and in vitro experiment using Stem cells and accompanying biomaterials. (issca.us)
  • These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
  • Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
  • Cells in vivo experience a complex, three-dimensional environment that exposes them to circulating molecules, neighboring cells, and the extracellular matrix (ECM). (mimetas.com)
  • Meanwhile, 3D cell culture systems feature increased complexity for increased faithfulness to the in vivo environment. (mimetas.com)
  • Magown P., Shettar B., Zhang Y. and Rafuse V.F. Â Direct optical activation of skeletal muscle fibers efficiently controls muscle contraction and attenuates denervation atrophy due to injury. (amap.ca)
  • A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
  • To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
  • The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
  • PAK1 has also been implicated for maintenance of glucose homeostasis in pancreatic beta cells and skeletal muscle. (thehealthyplanet.com)
  • Mitochondrial function or the ability to generate energy through OXPHOS (oxidative phosphorylation) is vital for cell homeostasis and its dysfunction has been linked to the pathogenesis of nearly all chronic diseases ( Pieczenik and Neustadt, 2007 ). (frontiersin.org)
  • Also, it is the most characterized embryonic carcinoma (EC) cell line that can be induced into cardiac muscle cells and neuronal cells by different specific treatments. (wikipedia.org)
  • When treated with DMSO, cardiac muscle cells developed after 5 days of exposure and skeletal muscle cells appeared after 8 days of exposure. (wikipedia.org)
  • We discovered that the cellular energy sensor AMP kinase (AMPK) that augments glycolysis during stress in cardiac and skeletal muscle is co-opted by GSCs for optimal glycolysis. (grantome.com)
  • However, researchers utilize these cells to study the development of cancer cells in order to find more specific treatments. (wikipedia.org)
  • Researchers have discovered an extracellular ligand that counteracts muscle wasting in cachexia. (leibniz-gemeinschaft.de)
  • Researchers from the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) in Jena, Germany, show that the extracellular ligand Wnt7a effectively counteracts muscle wasting through activation of the anabolic AKT/mTOR pathway and thereby reverts the loss of muscle stem cell functionality and muscle mass. (leibniz-gemeinschaft.de)
  • Previous attempts to model protocells using microcapsules have fallen short, so the team of researchers turned to bacteria to build complex synthetic cells using a living material assembly process. (nanowerk.com)
  • In the first report [2], researchers from Edinburgh and Oxford took cells from the mouse brain marked with transgene 1, and cultured them together with ES cells marked with a second transgene, 2. (i-sis.org.uk)
  • These researchers pointed out that the spontaneous fusion rate (without interleukin-3) was extremely low, between 2-11 per million bone marrow cells, and is unlikely to account for all the findings with adult stem cells. (i-sis.org.uk)
  • Another issue the researchers investigated was whether these neural-like cells would form tumors, a characteristic of many types of stem cells. (wakehealth.edu)
  • The researchers collected whole blood samples from the astronauts 10 days before their flight and on the day of landing, and white blood cells only three days after landing. (spaceref.com)
  • Along with microfluidic or organ-on-a-chip systems , 3D cell culture offers researchers an unparalleled ability to recreate physiological compositions and spatial arrangements of cells in vitro . (mimetas.com)
  • Therefore, FMOD reprogramming provides an alternative strategy for engineering patient-specific multipotent cells for basic research and therapeutic application. (ca.gov)
  • A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)
  • Those studies showed that drug exposure causes multipotent P19 cells to differentiate into different layers of cells. (wikipedia.org)
  • The research group "Stem Cells of Skeletal Muscle" lead by Dr. Julia von Maltzahn at the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) in Jena, Germany, investigated the extracellular ligand Wnt7a and was able to show its positive influence on muscle mass and muscle stem cells in cancer cachexia independently of the tumor cell type causing cachexia. (leibniz-gemeinschaft.de)
  • The extracellular ligand Wnt7a activates the anabolic AKT/mTOR pathway in myofibers and reverts the loss of muscle stem cell functionality. (leibniz-gemeinschaft.de)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • In the latest skirmish, two rapid online publications are presented as if they were evidence against the existence of adult stem cells. (i-sis.org.uk)
  • Two reports appeared as advance online publications in the top British journal Nature , accompanied by a news report that begins, "The hyped ability of adult stem cells to sprout replacement tissue types is being called into question. (i-sis.org.uk)
  • This leaves one with the distinct impression that adult stem cells might not exist, and furthermore, they may be a health hazard. (i-sis.org.uk)
  • In fact, the senior authors in both reports admit that some adult stem cells may genuinely revert to an earlier stage of development or switch fates [1]. (i-sis.org.uk)
  • The finding that adult cells can fuse with ES cells is therefore irrelevant to the debate, and the fact that it was used in an attempt to discredit adult stem cells is itself revealing, particularly in view of other recent findings on ES cells. (i-sis.org.uk)
  • This latest anti-publicity on adult stem cells comes on the heels of a paper announcing success in embryonic stem (ES) cell transplant in a Parkinson rat model published in the house journal of the United States National Academy of Sciences [5]. (i-sis.org.uk)
  • These adult stem cells are highly specialized and can only produce the tissue in which they reside. (pas.va)
  • Since giving the entire iPSC could result in tumor production, Xuan thinks and has early evidence that for strictly age-related muscle loss, just giving the vesicles produced by her process is likely the best strategy - and medicine - to help regenerate aged stem cells and restore more resilient, youthful muscle. (augusta.edu)
  • After 10 days of exposure, astroglial cells can be detected using glial fibrillary acidic protein (GFAP), which is a specific marker of glial cells. (wikipedia.org)
  • This makes Wnt7a a promising candidate for the treatment of muscle wasting diseases also in humans, since the protein has the same function in mice and men. (leibniz-gemeinschaft.de)
  • In the second report [3], mouse bone marrow cells marked with green fluorescent protein were found to fuse with ES cells in culture in the presence of the cytokine, interleukin-3, which is known to encourage cell fusion. (i-sis.org.uk)
  • The systems have been shown to improve cell proliferation and specific protein expression beyond that seen for static 3D cultures [4,5,6,7] . (reprocell.com)
  • The levels of glucose and secreted lactate contained in the cell culture medium were monitored as indicators of metabolic depletion, while protein syntheses and cell proliferation were measured by expression of phosphorylated Ribosomal protein S6 and cyclin D1. (reprocell.com)
  • Results suggested that perfusion culture reduces hypoglycemia, anaerobic stress, and preserves protein synthesis and cell proliferation. (reprocell.com)
  • Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for Cdkn2a ( p16 Ink4a ) repression. (elifesciences.org)
  • As reported in prior studies, we created a fluorescent protein-based sign program to monitor the cell routine status, known as the fluorescent ubiquitination-based cell routine Asenapine maleate sign (Fucci)20,21. (e-core.org)
  • In today's research, to visualize the cells in the G0 stage, we transduced a probe, utilizing a fusion protein between your fluorescent protein mVenus and a p27K? (e-core.org)
  • probe that recognizes quiescent cells To build up fluorescent probes that visualize the cells in the G0 stage, we initial fused mVenus towards the N-terminus of outrageous type p27 and retrovirally transduced the ensuing fusion protein (mVenus-p27WT) to NIH3T3 cells. (e-core.org)
  • These molecular features are in keeping with the type of quiescent cells as lately reported, helping the feasibility of our bodies. (e-core.org)
  • This product's molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment. (advancedbiomatrix.com)
  • The cell and molecular mechanisms that underlie developmental motoneuron cell death. (edu.au)
  • Nuclear lamina is a fundamental structure of the cell nucleus and regulates a wide range of molecular pathways. (bvsalud.org)
  • On the other hand, molecular oscillation and its phase resetting mechanism were intact in both the Lmna-deficient cells and progeria-mimicking cells. (bvsalud.org)
  • Normalization of YAP1 expression reduces tumor burden in human ERMS xenografts and allows YAP1-driven ERMS to differentiate in situ. (elsevierpure.com)
  • Therefore, when switched to a low glucose medium (that reduces glycolysis), cancer cells become sensitized to metformin. (grantome.com)
  • We will test if genetic inhibition of AMPK in GSCs reduces glycolysis, suppress proliferation and tumor growth and improves metformin sensitivity. (grantome.com)
  • DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • Givinostat primes them, it tunes them to produce the right type of vesicles," says coinvestigator Dr. Mark Hamrick , a bone and muscle biologist in the MCG Department of Cellular Biology and Anatomy and MCG's senior associate dean for research. (augusta.edu)
  • The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs. (tocris.com)
  • We found that some methods to generate muscle cells in a dish also produce unique cell types that likely support the muscle cells," said Pyle, who is also a member of the UCLA Jonsson Comprehensive Cancer Center. (scienceblog.com)
  • Cancer cells in humans may result in the patient's death if the aggressive cancer cell grows and metastasizes. (wikipedia.org)
  • Wnt7a is able to prevent muscle loss in cancer cachexia making it a potential candidate to be used during treatment or surgeries. (leibniz-gemeinschaft.de)
  • Neither report cited a paper published last year in the journal Blood [4], where a group from the Stem Cell Institute, Department of Medicine, and Cancer Center, University of Minnesota Medical School, Minneapolis, reported the most comprehensive experiments proving that a single adult stem cell can differentiate into all cell types in culture. (i-sis.org.uk)
  • Swedish Research Counsil (R.J.A.N.), National Cancer Institute (NCI) CA069246 and CA86355 (X.O.B.), and the American Brain Tumor Association (T.W.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
  • In this post, we will explore two examples of how perfusion systems can help improve the relevance of your 3D cell culture, based on two publications from Alvetex users in the field of developmental biology and cancer. (reprocell.com)
  • In a separate project, scientists from the University of Oxford cultured the human pancreatic cancer cell line PSN-1 over a period of seven days, in either static or perfused conditions, using the Alvetex perfusion plate . (reprocell.com)
  • Cancer Cell , 26 (2), 273-287. (elsevierpure.com)
  • In this proposal we will determine how to enhance cancer cell sensitivity towards metformin therapy. (grantome.com)
  • There is a renewed interest to examine mitochondrial function in human cancer following recent discoveries that tumor cell mitochondria regulate various aspects of tumorigenesis. (grantome.com)
  • A physician who heard my lecture at one of the conferences told me that Ivermectin has anti-cancer properties and anti-cancer stem cell properties. (thehealthyplanet.com)
  • Cancer stem cells are the reason why cancer cells often develop resistance to chemotherapies. (thehealthyplanet.com)
  • These researches are telling us that a combination of chemo agents with Ivermectin is targeting cancer stem cells. (thehealthyplanet.com)
  • Killing cancer stem cells is the holy grail of cancer therapy. (thehealthyplanet.com)
  • Bauer Research Foundation published on December 12, 2012 that Praziquantel , my other favorite parasite medication for liver flukes, synergistically enhances Paclitaxel (Taxol) efficacy to inhibit cancer cell growth (PloS ONE 7(12): e51721). (thehealthyplanet.com)
  • Taxol is a common chemo agent for ovarian cancer, breast cancer, small cell lung cancer, head and neck cancer, esophageal cancer, prostate and bladder cancer. (thehealthyplanet.com)
  • The INK4b-ARF-INK4a tumour suppressor locus controls the balance between progenitor cell renewal and cancer. (biomedcentral.com)
  • The INK4b-ARF-INK4a tumor suppressor locus (Figure 1A ) plays a central role in controlling the equilibrium between progenitor cell renewal and cancer risk [ 1 - 8 ]. (biomedcentral.com)
  • Identification of invadopodia by TKS5 staining in human cancer lines and patient tumor samples. (advancedbiomatrix.com)
  • The overall goal of the lab is to advance an understanding of the vertebrate body plan via the zebrafish embryos and provide insights to understand stem cell biology and mechanisms of cancer metastasis. (stonybrook.edu)
  • these studies will lay the foundation for using this new technology to restore meaningful movement, such as hand grasping or breathing, to individuals whose hand muscles or diaphragm were permanently paralyzed due to motor neuron death after a spinal cord injury. (amap.ca)
  • The grant enables Martin and colleagues to carry out research designed to more clearly define and observe neuromesodermal progenitors, stem cells that contribute to spinal cord or skeletal muscle development. (stonybrook.edu)
  • Neuromesodermal progenitors exist in all vertebrate embryos, so zebrafish are used as a common model to these cells' development to better define how the embryonic body plan is formed and how spinal cord and skeletal muscle are induced from this population. (stonybrook.edu)
  • However, until now, they had no way of determining where these cells fall on the continuum of human development. (scienceblog.com)
  • So we set out to generate this map as a reference that our lab and others can use to compare the genetic signatures of the cells we are creating to those of real human skeletal muscle tissue. (scienceblog.com)
  • The group obtained 21 samples of human skeletal muscle tissue from their UCLA collaborators and from colleagues at the University of Southern California and the University of Tübingen in Germany. (scienceblog.com)
  • This enabled the group to match the genetic signatures found in the pluripotent stem cell-derived muscle cells with their corresponding locations on the map of human muscle development. (scienceblog.com)
  • We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
  • These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
  • This strategy has been applied to develop a three-dimensional (3D) human muscle model with endothelium specificity and endomysium for the study of fibrosis 22 . (nature.com)
  • We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
  • Expressionof estrogen receptor-alpha and -beta, glucocorticoidreceptor,and progesterone receptorgenes in human embryonic stemcells andembryoid bodies. (miraewa.com)
  • Availablehuman feeder cells for the maintenance of human embryonicstemcells. (miraewa.com)
  • Establishmentand maintenance of human embryonic stem cell lineson human feedercells derived from uterine endometrium under serum-freecondition. (miraewa.com)
  • Neither the title of the paper, nor the abstract mentioned that in the experiment, five out of 25 rats receiving the transplant died with "teratoma-like tumors" in their brains, a well-known hazard of ES cells. (i-sis.org.uk)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • In particular, this project is aimed at looking at the signalling pathways that are activated when these growth factors activate their receptors, which are on these stem and tumor cells. (edu.au)
  • MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)
  • My research focuses on investigating the mechanisms responsible for sarcopenia (the age-related loss of skeletal muscle mass and strength) and developing exercise interventions for the treatment/prevention of sarcopenia. (uncg.edu)
  • This involves exploring the signal transduction mechanisms that are activated when signalling molecules such as neuregulin-1 (ARIA, beta-heregulin 1), or agrin active ErbB or MuSK receptors in the membrane of muscle respectively. (edu.au)
  • This course and the tendency for recurrence make the treatment of these relatively rare fibrous tumors challenging. (medscape.com)
  • Cell cultures containing undifferentiated stem cells were isolated from the primary tumor which have a euploid karyotype. (wikipedia.org)
  • Have 4 connected wells, allowing for greater volume provision for highly metabolic monocultures, or active solute flow for paracrine or cell migration co-cultures. (reprocell.com)
  • Could perfusion reduce metabolic stress in your cultures and improve the functionality of your cells? (reprocell.com)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • This makes 3D cell cultures appealing. (mimetas.com)
  • Quantificationof tumor suppressor mRNA expression by poly-competitiveRT-PCRusing a TS-IS thatcontained multiple internal competitors. (miraewa.com)
  • In an earlier study, the Wake Forest Baptist team isolated neural precursor cells derived from skeletal muscle of adult transgenic mice (PLOS One, Feb.3, 2011). (wakehealth.edu)
  • In mice, sustained YAP1 hyperactivity in activated, but not quiescent, satellite cells induces ERMS with high penetrance and short latency. (elsevierpure.com)
  • In contrast, pre-GEPCOT cells were quiescent, expressed higher Glast, and lower EGFR and PlexinB2. (elifesciences.org)
  • useful in looking into stem cells aswell as quiescent cells. (e-core.org)
  • The quiescent cells have already been regarded as dormant, waiting around to enter the cell routine3,4,5. (e-core.org)
  • The known degrees of p27 are higher in quiescent cells than in bicycling cells30,31,32,33. (e-core.org)
  • Nevertheless, this marker could recognize and isolate the quiescent cells. (e-core.org)
  • ATGL in beta cells regulates insulin secretion via the production of signalling MAGs. (omicsdi.org)
  • Progressive, age-related muscle loss is called sarcopenia, and it can impair mobility and quality of life, says Dr. Wanling Xuan , stem cell biologist in the Vascular Biology Center at the Medical College of Georgia . (augusta.edu)
  • In their animal model of aging-related sarcopenia, they've already shown the strategies increase the number of muscle stem cells and decrease destructive inflammation, which typically increases with age. (augusta.edu)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • In 1982, McBurney and Rogers transplanted a 7.5 day mouse embryo into the testis to induce tumor growth. (wikipedia.org)
  • Treatment of undifferentiated P19 cells with retinoic acid can specifically induce them into neuronal cells. (wikipedia.org)
  • At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
  • The paper states Ivermectin synergizes with chemo agents cytarabine and daunorubicin to induce cell death in leukemia cells. (thehealthyplanet.com)
  • Xuan and longtime mentor Dr. Muhammad Ashraf , professor and stem cell biologist in the Vascular Biology Center, have a new paper in the journal Stem Cell Research & Therapy indicating that using givinostat to induce iPSCs into action helps replenish satellite cells in the face of muscular dystrophy. (augusta.edu)
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)