Skeletal muscle proliferation and differentiation myogenesis satellite cells in human skeletal muscle. Medical search. Frequent questions

C2C12 cells, primary myoblasts and myotubes. (sun.ac.za)
Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)
These findings imply a novel and unexpected role for p38 MAPK inhibition in myogenesis and highlight the importance of the limb bud microenvironment in promoting the progression of limb myoblasts. (biologists.com)
These monocyte myoblasts proliferate and differentiate, following which, they fuse with existing muscle fibers to form multinucleated myotubes and myofibers. (kosfaj.org)

Although Myf5 is mainly associated with myogenesis, it is expressed in other tissues, as well. (wikipedia.org)
Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
Comparison of transcriptome of skeletal muscles and other tissues between phenotypically different pig breeds has been proposed to improve the understanding of the genetic mechanisms underlying differences in growth and meat quality [ 10 ]. (biomedcentral.com)
What was surprising is that administering these inhibitors to the whole animal appears to reduce TGF-beta levels in the whole animal, suggesting some kind of feed-back and perhaps effects on other tissues as well as muscle. (ca.gov)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
MSCs can be isolated from a range of tissues, but the most commonly cited sources are bone marrow (BM), adipose tissue, muscle, bone, and perinatal tissues (e.g. (biomedcentral.com)
Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. (u-paris.fr)
A central aspect is then the ability to optimize the functional properties with high spatial resolution, creating materials that are able to control the interaction with the biological surrounding at the nanoscale thus guiding the responses of biomolecules, cells and tissues. (european-mrs.com)
Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
We have chosen to work on human precursors, either in vitro or in vivo, in xenograft models that we have developed in successive European networks. (institut-myologie.org)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)
Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)
In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)

We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)

Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
On the other hand, despite the atrophy and reduced myogenic potential of the EDL muscles in old rats, when an EDL muscle is cross-age transplanted from an old donor rat into a young host rat, the muscle regenerates as much mass and has as high a capacity for force development as a young muscle transplanted into a young host [4] . (josam.org)
Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
The effect of deer antler extract on muscle differentiation and muscle atrophy were evaluated to minimize muscle loss following aging. (kosfaj.org)
In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. (kosfaj.org)
Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. (kosfaj.org)
In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the CON. (kosfaj.org)
Therefore, our findings indicate that antler extract can increase the expression of MyoD1, Myf5 and myogenin, inhibit muscle atrophy, and promote muscle differentiation. (kosfaj.org)
Muscle atrophy with aging is accompanied by muscle damage caused by oxidative damage and malnutrition caused by lack of muscle metabolism. (kosfaj.org)

In this project, the group intends to investigate the nature and the role of adenosine and AR signalling pathways on the two isoforms of muscle AChRs: the embryonic (g-AChR) regulating synaptogenesis and muscle development and the adult (e-AChR) with a role in nerve-muscle communication. (units.it)
Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)
Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)

Still, the existence of these separate "MyoD-dependent" and "Myf5-dependent" subpopulations has been disputed, with some claiming that these MRFs are indeed coexpressed in muscle progenitor cells. (wikipedia.org)
We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)
Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)

First, to what extent are the satellite cells from aged mammals capable of fusion into functional myotubes, and second, do satellite cells from aged mammals have any form of impaired function when compared with those from young or adult mammals? (josam.org)
In the presence of antler extracts, the length and thickness of myotubes and myogenin differentiation 1 (MyoD1) and myogenic factor 5 (Myf5) gene expression were increased compared to those in the control group (CON). (kosfaj.org)

Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus, Bmc Genomics, 20, 156, https://doi.org/10.1186/s12864-019-5530-7, 2019. (copernicus.org)

Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
Therefore, promotion of myoblast proliferation and differentiation and induction of myotube hypertrophy should be beneficial for muscle regeneration and control of muscle mass. (kosfaj.org)

One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)

In more detail, the team is studying the role of electrical activity and local trophic factors in the microenvironment of satellite cell niche and in the post-mitotic myogenesis. (units.it)
We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)

Some evidence shows that Myf5 and MyoD are responsible for the development of separate muscle lineages, and are not expressed concurrently in the same cell. (wikipedia.org)
As no muscle is formed in Myf5;Myod double mutant zebrafish, Myf5 cooperates with Myod to promote myogenesis. (wikipedia.org)
All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)

As one of the key cell markers of satellite cells (the stem cell pool for skeletal muscles), it plays an important role in the regeneration of adult muscle. (wikipedia.org)
We determined the true SC state needed to repopulate after reinjury- the gold standard requirement for maintaining stem cell function long term. (ca.gov)
The research in this propoal will benefit CA residents tremendously by understanding how to generate a muscle stem cell capable of responding to injury and undergoing long term repopulation after injury to generate new muscle. (ca.gov)
Cell Stem Cell 2013;13(5):590-601. (scielo.br)
Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)
KIT is a receptor tyrosine kinase type III, which binds to stem cell factor. (inhibitorkit.com)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)

Although it is expressed in both the epaxial (to become muscles of the back) and hypaxial (body wall and limb muscles) portions of the myotome, it is regulated differently in these tissue lines, providing part of their alternative differentiation. (wikipedia.org)
The formation of skeletal muscle:from somite to limb. (scielo.br)
Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)

IL-2/IL2R also promotes the differentiation of T cells into effector T cells. (ilreceptor.com)

We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)

General, Hep3B cells appeared to be more sensitive towards the DACi mediated inhibition of DNA methylation as shown by a significant and robust reduction of methylated APC after only six h. (ilreceptor.com)

We will molecularly and functionally define muscle stem cells in human muscle in development, juvenile and adult and develop strategies to generate the most regenerative muscle stem cells from hPSCs. (ca.gov)
Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)
In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)
However, discoveries in our lab have also shown that regulatory interactions between cytotoxic, M1 macrophages in dystrophic muscle and anti-inflammatory, M2 macrophages are important in regulating the balance between the death of dystrophic muscle and regenerative processes. (ucla.edu)

In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
In addition to regulating physiological nitric oxide signaling and responses of cells to stress, studies in mice lacking thrombospondin-1 or its receptors have revealed important roles for thrombospondin-1 in regulating immune responses in infectious and autoimmune diseases and antitumor immunity. (bvsalud.org)
in these cells physiological lev els of E2 and low levels of xenoestrogens can rapidly reverse actions of the DAT. (inhibitorkit.com)
In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)
Skeletal muscle is characterized by a remarkable capacity to rearrange after physiological changes and efficiently regenerate. (biomed.news)

It functions during that time to commit myogenic precursor cells to become skeletal muscle. (wikipedia.org)

Cell Metabolism 2013;17(2):210-24. (scielo.br)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
We observed alteration in biological functions related to extracellular matrix function and organization, cellular adhesion, muscle growth, lipid metabolism and proteolysis. (biomedcentral.com)

More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
She has been reviewer for international Journals ( FASEB J , Cell & Tissue Res , Toxicon, Exp Cell Res ), for national grant agencies (MIUR) and international Foundations (AFM, Association Francaise contre les Myopathies). (units.it)
With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)
In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
While all vertebrates are capable of some types of tissue regeneration, most, including humans, have lost the ability to regenerate whole structures such as limbs ( epimorphic regeneration ), [ 1 ]. (biomedcentral.com)

Differentiation begins (regulated by other genes) after this initial proliferation. (wikipedia.org)
The objective of this work was the evaluation of muscle transcriptome profile in piglets of both genetic types, in order to identify genes, pathways and regulatory factors responsible for their phenotypic differences. (biomedcentral.com)
Candidate genes with known effects on muscle growth were found among the DE genes upregulated in DU×IB. (biomedcentral.com)

Myogenic factor 5 is a protein that in humans is encoded by the MYF5 gene. (wikipedia.org)
It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. (wikipedia.org)
The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)
We observed that, as skeletal muscle progressed from perinatal to postnatal and adult developmental stages, MSY3 protein became gradually dephosphorylated and accumulated in the nucleus. (biomedcentral.com)
These findings had been corroborated by westernblot analysis displaying a powerful reduction of DNMT1 and DNMT3a protein in each cell lines but not of DNMT3b. (ilreceptor.com)
Right here, only a transient decrease in protein levels was observed right after 24 to 48 h in both cell lines. (ilreceptor.com)
Mammalian hibernation presents a unique scenario, where animals are hypocaloric and in torpor, but the extent of muscle protein loss is minimized. (silverchair.com)
The poultry sector is noted to make a considerable contribution to global nutrition and food security, which helps in the provision of cheap protein, essential micronutrients, and energy to humans ( 1 ). (frontiersin.org)
however, priority needs to be given to resistance exercise training, and secondarily to adequate protein intake should be part of this dietary regime to mitigate losing muscle mass. (biomed.news)
Other NIH-funded investigations in our lab explore epigenetic mechanisms through which an anti-aging protein called Klotho affects myogenesis and muscle regeneration in neonatal and aging muscle. (ucla.edu)

Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)

In C2C12 myogenic cells, blocking the IGF1/PI3K/Akt pathway using LY294002 inhibitor reduced MSY3 phosphorylation levels resulting in its accumulation in the nuclei. (biomedcentral.com)

The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)

A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)
In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
Evaluate myogenic ability and functional differences of embryonic, fetal, juvenile and adult MuSCs in fusion, proliferation, engraftment and repopulation assays in mouse models of muscle disease. (ca.gov)
Develop transcription factor over-expression in combination with improved directed differentiation strategies to generate MuSCs from hPSCs with genetic and functional signatures of the in vivo MuSCs. (ca.gov)
Several types of adenosine receptors have been detected in developing and differentiated skeletal muscle cells, but little is known about the functional outcome of AR modulation on muscle AChRs. (units.it)
We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)

Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)
Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)

Knocking down Akt expression increased the amount of dephosphorylated MSY3 and reduced myogenin expression and muscle differentiation. (biomedcentral.com)
While Akt over-expression rescued myogenin expression in MSY3 overexpressing myogenic cells, ablation of the Akt substrate, (Ser126 located in the MSY3 cold shock domain) promoted MSY3 accumulation in the nucleus and abolished this rescue. (biomedcentral.com)
Furthermore, forced expression of Akt in adult skeletal muscle induced MSY3 phosphorylation and myogenin derepression. (biomedcentral.com)
These results support the hypothesis that MSY3 phosphorylation by Akt interferes with MSY3 repression of myogenin circuit activity during muscle development. (biomedcentral.com)
This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)
Given the significance of myogenin regulation in adult muscle, the Akt/MSY3/myogenin regulatory circuit is a potential therapeutic target to counteract muscle degenerative disease. (biomedcentral.com)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
Although adipocyte differentiation starts early in fetal stages, the increase in adipose cell number and size is maintained along early postnatal growth and later development [ 6 ]. (biomedcentral.com)

Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)

We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)
Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

This review concentrates on the research progress of the non-canonical Hippo pathway in regulating innate immune cell anti-infection responses, maintaining redox homeostasis, responding to microenvironmental stiffness, and T-cell differentiation. (bvsalud.org)
The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
Exploring the mechanisms through which the immune system can modulate skeletal muscle wasting, injury, regeneration and growth is a particular focus of the lab. (ucla.edu)
Discoveries in the our lab over the past 25 years have shown that immune cells, especially myeloid cells, play a major role in modulating muscle injury and repair that occur in chronic, muscle wasting diseases and following acute injuries. (ucla.edu)

6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
Possible MSY3 mechanisms of action have been studied in cell culture studies. (biomedcentral.com)
Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)

The main interest of her research activity is the nerve-muscle communication and the skeletal muscle physiology, including muscle plasticity and impact of ageing on the neuromuscular system. (units.it)
The results of the proposed research will advance the understanding of important aspects of the neuromuscular physiology such as the modulation of the neuromuscular transmission and the skeletal muscle plasticity. (units.it)

We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)
Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)

In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)

Firstly, such modeling methods give an insight into how cell parts function under stress. (globaltechbiz.com)

ont permis la publication de plus de 370 articles et monographies, dont une 44 en 2020. (u-paris.fr)

While the product of this gene is capable of directing cells towards the skeletal muscle lineage, it is not absolutely required for this process. (wikipedia.org)
To understand the function of SFRP1 and miRNA-1/206 in swine myogenesis, we first predicted the targets of miRNA-1/206 with the TargetScan and PicTar programs, and analyzed the molecular characterization of the porcine SFRP1 gene. (biomedcentral.com)
Circular transcripts of the testis-determining gene Sry in adult mouse testis, Cell, 73, 1019-1030, https://doi.org/10.1016/0092-8674(93)90279-y, 1993. (copernicus.org)
Animals with a polymorphism in the myostatin gene that renders it less active have hypertrophied muscles. (silverchair.com)
Duchenne muscular dystrophy (DMD), the most common and severe form of muscular dystrophy, is caused by mutations in the dystrophin gene, the largest gene identified in the human genome. (biomedcentral.com)

Myooids formed by self-organization of myogenic cells in the absence of an artificial scaffold and were maintained in culture. (josam.org)
We hypothesize that the remaining force deficit is due to cellular and sub-cellular disorganization-Myooids lack the density and organization of sarcomeric arrays seen in skeletal muscle. (josam.org)
Basic helix-loop-helix (bHLH) myogenic regulatory factors coordinate the correct function and organization of many skeletal muscle functions. (biomedcentral.com)
Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion. (u-paris.fr)

Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)
When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
Thrombospondin-1 is a secreted matricellular glycoprotein that modulates cell behavior by interacting with components of the extracellular matrix and with several cell surface receptors. (bvsalud.org)
Its presence in the extracellular matrix is induced by injuries that cause thrombospondin-1 release from platelets and conditions including hyperglycemia, ischemia, and aging that stimulate its expression by many cell types. (bvsalud.org)
Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)
These MSC influences can range from relatively rare activities that require cell contact, such as mitochondrial transfer and cell fusion, to relatively common paracrine MSC actions through extracellular microvesicles or secreted factors. (biomedcentral.com)

Introduction Cell mechanics has proved to be a widely used label-free biomarker to discern phenotypes, detect pathologies and more importantly, monitor existence or progression of a disease [1C3]. (globaltechbiz.com)
Secondly, they can improve our understanding of the adjustments that occur during disease progression which, in turn, might uncover reasons for corresponding alterations occurring in cell mechanics [10, 11]. (globaltechbiz.com)

Cellular and molecular regulation of muscle regeneration. (scielo.br)
Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)

Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)

Panobinostat therapy significantly repressed mRNA for DNMT1 and DNMT3a in the two cell lines while no modifications had been observed in DNMT3b levels. (thrombininhibitors.com)
Panobinostat treatment drastically repressed mRNA for DNMT1 and DNMT3a in the two cell lines whilst no adjustments have been observed in DNMT3b levels. (ilreceptor.com)

These cells are regulated by a tight control of transcriptional programs. (biomed.news)

Although the specific roles and dependency of Myf5 in adipogenesis and neurogenesis have remained to be explored, these findings show that Myf5 may play roles outside of myogenesis. (wikipedia.org)
For example, our findings have shown that macrophages and eosinophils are key effector cells in the pathogenesis of Duchenne muscular dystrophy. (ucla.edu)

In fact, if Myf5 is not downregulated, differentiation does not occur. (wikipedia.org)
Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)

Most notably, while Myf5 is activated by Sonic hedgehog in the epaxial lineage, it is instead directly activated by the transcription factor Pax3 in hypaxial cells. (wikipedia.org)

These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
Within skeletal muscle, lipids are stored as droplets both in myofiber cytoplasm and in adipocytes (interspersed between fiber fasciculi) with IMF deposition being highly dependent on the number of intramuscular adipocytes [ 5 ]. (biomedcentral.com)

In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Furthermore, Myf5 is a master regulator of muscle development, possessing the ability to induce a muscle phenotype upon its forced expression in fibroblastic cells. (wikipedia.org)
The high redundancy of this system shows how crucial the development of skeletal muscle is to the viability of the fetus. (wikipedia.org)
Despite only being present for a few days during embryonic development, Myf5 is still expressed in certain adult cells. (wikipedia.org)
Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)
Moreover, miRNAs play a modulatory role in the development and growth of skeletal muscles [ 22 ]. (biomedcentral.com)

Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)

These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
The enteric nervous system (ENS) is principally derived from vagal neural crest cells that migrate caudally along the entire length of the gastrointestinal tract, giving rise to neurons and glial cells in two ganglionated plexuses. (bvsalud.org)
Incomplete migration of enteric neural crest-derived cells (ENCDC) leads to Hirschsprung disease, a congenital disorder characterized by the absence of enteric ganglia along variable lengths of the colorectum. (bvsalud.org)

In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)

In this light, they will be useful for identification of new pharmacological tools to control the activity of muscle AChRs and, thus, for finding novel strategies for neuromuscular diseases associated with altered neuromuscular transmission. (units.it)
The IAL aims to synergise the expertise in muscle and neuromuscular diseases of French teams and the expertise in immunology and inflammation of the Brazilian teams . (institut-myologie.org)

Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)

For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)
Primary MuSCs isolated from individual skeletal muscles (gastrocnemius and masseter) are lysed, and their RNA is reverse-transcribed into cDNA and copied into double-stranded DNA. (biomed.news)

The absence of all three of these factors results in a phenotype with no skeletal muscle. (wikipedia.org)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)

Anatomy57

Organisms13

Diseases17

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes58

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena7

Information Science5

Health Care1

Myoblasts5

Tissues13

Vitro10

Myofibers11

Porcine skeletal muscle1

Atrophy9

Isoforms6

Progenitor5

Myotubes2

20191

Myoblast2

Regeneration of skeletal muscle1

Microenvironment3

Myod3

Stem cell9

Limb4

Effector T cel1

Pluripotent Stem1

Inhibition1

Regenerative9

Physiological6

Precursor cells1

Metabolism3

Tissue14

Genes3

Protein10

Dystrophic muscle3

C2C121

Rudnicki2

Functional11

Affects2

Myogenin6

Adipose2

Stimulation3

Vivo4

Immune4

Repair and regeneration1

Mechanisms4

Plasticity2

Adult muscle2

Murine3

Methods1

20201

Gene5

Organization4

Extracellular6

Progression2

Regulation3

Progenitors1

MRNA2

Transcriptional1

Findings2

Occur2

Pax31

Myofiber4

Fibroblasts1

Development6

Therapeutic1

Neural3

Embryonic stem1

Neuromuscular2

Mesodermal1

MuSCs2

Phenotype2