Loading...
  • atrophy
  • Since exercise can improve muscle strength and endurance capacity, it can serve as a means of preventing muscle atrophy and reducing the risk of sarcopenia. (hindawi.com)
  • P. O. Mitchell and G. K. Pavlath, "Skeletal muscle atrophy leads to loss and dysfunction of muscle precursor cells," The American Journal of Physiology-Cell Physiology , vol. 287, no. 6, pp. (hindawi.com)
  • In contrast to counterparts from young subject's muscle, the satellite cells from aged muscle exhibit the gene expression pattern which favours oxidative damage, altered turnover of cytoskeleton, activation of FOXO-dependent atrophy programs, suppressed myotube formation, and impairment of [Ca.sup.2+]-mediated signaling [25-27, 29, (thefreelibrary.com)
  • therapeutic
  • An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. (nature.com)
  • The search for knowledge on cellular and molecular mechanisms involved in skeletal muscle mass homeostasis and regeneration is an exciting scientific area and extremely important to develop therapeutic strategies for neuromuscular disorders and conditions related to muscle wasting. (frontiersin.org)
  • It is only through a more thorough understanding of this cell population that their therapeutic potential be realized. (mcmaster.ca)
  • FAPs
  • FAPs also represent a major cellular source of fibrosis in degenerative disease states, highlighting them as a potential cellular target for anti-fibrotic muscle therapies. (springer.com)
  • Together, this protocol provides a complete workflow to study skeletal muscle derived FAPs and compliments downstream analytical, drug screening, and disease modeling applications. (springer.com)
  • neural
  • Moreover, we show that the ubiquitin ligase TRIM32 controls this process through the regulation of c-Myc, a similar mechanism to that previously observed in neural progenitors. (purdue.edu)
  • transcriptional
  • This Research Topic aims to highlight recent developments on the role of stem cells in skeletal muscle function, both in prenatal and postnatal life, and their regulation by transcriptional, post-transcriptional and epigenetic mechanisms. (frontiersin.org)
  • We are currently exploring further the regulation of the Notch and Wnt signaling pathways during satellite cell activation, the mechanisms underlying the transcriptional control of Delta expression, and epigenetic processes that may account for age-related changes in these pathways. (stanford.edu)
  • Current studies are focused on the role of post-transcriptional regulation of stem cell quiescence and activation. (stanford.edu)
  • First, we are using microarray and next-generation high throughput sequencing to derive molecular signatures of young and old stem cells and the transcriptional and epigenetic levels. (stanford.edu)
  • molecular
  • However, the molecular mechanisms involved in signaling integration within the satellite niche are still poorly understood. (rupress.org)
  • Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb," EMBO Molecular Medicine , vol. 3, no. 12, pp. 742-754, 2011. (hindawi.com)
  • Charge, S. B. & Rudnicki, M. A. Cellular and molecular regulation of muscle regeneration. (nature.com)
  • Using molecular, cellular and physiological techniques, we are attempting to define the regulation of this cell population in health and disease.Techniques used in the lab include: histology, immunohistochemistry, protein and RNA expression assays, isolated single fibre and primary myoblast cultures, in situ muscle stimulation to assess contractile function, adenoviral mediated overexpression and/or silencing and metabolic enzyme assays. (mcmaster.ca)
  • leads
  • Since constitutive mutation of Ppardelta leads to embryonic lethality, we sought to address this question by conditional knockout (cKO) of Ppardelta using Myf5-Cre/Ppardeltaflox/flox alleles to ablate PPARdelta in myogenic progenitor cells. (umassmed.edu)
  • acute
  • Previous studies have shown that the number of satellite cells is increased by long-term or acute exercise training in humans and animals [ 4 , 5 ] and decreases during aging in conjunction with a reduction in the muscle quality and functional potential [ 6 ]. (hindawi.com)
  • Preliminary efficacy data indicate that stem cells have the potential to enhance myocardial perfusion and/or contractile performance in patients with acute myocardial infarction, advanced coronary artery disease, and chronic heart failure. (ahajournals.org)
  • Our findings revealed that this scaffold system significantly promotes satellite cell activation in skeletal muscle and accelerates regeneration following acute muscle injury. (bilkent.edu.tr)
  • transcription
  • Gene expression analysis showed reduced expression of the Forkhead box class O transcription factor 1 (FoxO1) gene in Ppardelta-cKO muscles under both quiescent and regenerating conditions, suggesting that PPARdelta acts through FoxO1 in regulating muscle progenitor cells. (umassmed.edu)
  • The changes in satellite cell properties coincided with the inactivation of Notch signaling and the activation of Wnt signaling and likely involve modulation by transcription factors of the Sox family. (hindawi.com)
  • Although this scenario has yet to be demonstrated in satellite cells, it is most likely that analogous mechanisms would occur, and if so, it is possible that MyoD could be involved in inducing transcription from either Cdc6 or MCM2 in these cells after leaving quiescence. (rupress.org)
  • is a nuclear receptor-type transcription factor expressed mostly in skeletal muscle, and regulated by nutritional conditions. (jove.com)
  • proliferate
  • In response to muscle damage or under increased workload these cells activate, migrate into damaged sites, proliferate, and fuse with existing myofibers or with each other to form new myofibers, whereas a small portion of these cells returns into quiescence [11, (thefreelibrary.com)
  • They are normally found in silent state in adult muscle, but act as a reserve cell population that is able to proliferate in response to injury and give rise to regenerated muscle and to more satellite cells. (bilkent.edu.tr)
  • developmental
  • One of the most trusted assays to determine the developmental and regenerative potential of a given cell population relies on their isolation and transplantation 5-7 . (jove.com)
  • paracrine
  • Others have proposed that transient cell retention may be sufficient to promote functional effects, eg, by release of paracrine mediators. (ahajournals.org)