Skeletal molecular progenitor satellite cell. Medical search. Frequent questions

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)

Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Muscle satellite cells make up a stem cell populace that is capable of differentiating into myocytes and contributing to muscle mass regeneration upon injury. (healthyguide.info)
Stem cells are considered the most promising source for such meat, retaining as they do the capacity to transform into the required tissues, and the scientists pointed to satellite cells, which are the natural muscle stem cells responsible for regeneration and repair in adults. (livescience.com)
The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)
Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
Directional cell migration is a critical process underlying morphogenesis and post-natal tissue regeneration. (crick.ac.uk)
During embryonic myogenesis, migration of skeletal myogenic progenitors is essential to generate the anlagen of limbs, diaphragm and tongue, whereas in post-natal skeletal muscles, migration of muscle satellite (stem) cells towards regions of injury is necessary for repair and regeneration of muscle fibres. (crick.ac.uk)
Here, we review mechanisms of myogenic stem/progenitor cell migration during skeletal muscle development and post-natal regeneration. (crick.ac.uk)
Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration. (research.com)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
Following postdoctoral fellowships at the Center for Genomic Regulation (Barcelona, Spain) and the University of Colorado-Boulder, Dr. Doles moved to Mayo Clinic in 2016, where he leads a basic science research lab focused on stem cells, tissue regeneration, and multi-organ wasting. (jove.com)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
The research activity of the group is focused on the identification of new strategies to improve skeletal muscle regeneration. (units.it)
Remarkably type 2 cytokine signaling is also required in FAPs but not myeloid cells for quick clearance of necrotic debris a process that is necessary for timely and total regeneration of BV-6 cells. (scienceexhibitions.org)
2008 In addition to satellite cells recent studies have recognized an important part for fibro/adipogenic progenitors (FAPs) in muscle mass regeneration and its fatty degeneration (Joe et al. (scienceexhibitions.org)
In a number of species cells regeneration is associated with the presence of the molecular signature for type 2 innate immune response such as alternatively triggered (M2) macrophages and eosinophils (Allen and Wynn 2011 Palm et al. (scienceexhibitions.org)
Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
In particular, in skeletal muscle, specific semaphorin molecules are involved in the restoration and remodeling of the nerve-muscle connections, thus emphasizing their plausible role to ensure the success of muscle regeneration. (biomed.news)
This review article aims to discuss the impact of aging on skeletal muscle regeneration and NMJs remodeling and will highlight the most recent insights about the role of semaphorins in this context. (biomed.news)
Therefore, endogenous and exogenous erythropoietin contribute to increasing satellite cell number following muscle injury, improve myoblast proliferation and survival, and promote repair and regeneration in this mouse induced muscle injury model independent of its effect on erythrocyte production. (uzh.ch)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
In recent research, scientists unlocked ways to boost muscle regeneration in the experimental animal by activating the precursors of muscle cells. (sciencetrend.ca)
In recent research, scientists managed to activate the precursors of muscle cells, which contributes to aiding muscular regeneration and rebuilding damaged tissue. (sciencetrend.ca)
Salk Institute researchers are finding methods to speed up methods to accelerate the regeneration of muscle tissue, utilizing a combination of molecular compounds that are generally utilized in the stem-cell analysis. (sciencetrend.ca)
2021), the investigators confirmed that utilizing these compounds increased the regeneration of muscle cells in mice by activating the precursors of muscle cells, known as myogenic progenitors. (sciencetrend.ca)
Within the myofiber-specific model, they discovered that adding the Yamanaka factors accelerated muscle regeneration in mice by decreasing the levels of a protein referred to as Wnt4 in the area of interest, which activated the satellite cells. (sciencetrend.ca)
In contrast, Yamanaka factors didn't activate satellite cells within the satellite-cell-specific model and didn't enhance muscle regeneration, suggesting that Wnt4 performs an essential role in muscle regeneration. (sciencetrend.ca)
An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration. (sciencetrend.ca)
In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche. (sciencetrend.ca)

Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
Satellite cells from your trunk and the limb are derived from an embryonic populace of progenitor cells in the somites transient mesodermal constructions that develop on either part of the neural tube [1]. (healthyguide.info)
Interestingly recent data indicated that although MyoD is not indicated in quiescent satellite cells in the adult it is transiently indicated in satellite cell progenitors in the embryo suggesting that satellite cells are derived from committed embryonic precursors of myogenic lineage [4] [5]. (healthyguide.info)
Embryonic stem cells could also be used, but they are obviously plagued by ethical concerns, and they could grow into tissues besides the desired muscles. (livescience.com)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)

To grow meat in labs from satellite cells, the researchers suggested current tissue-engineering techniques, where stem cells are often embedded in synthetic three-dimensional biodegradable matrixes that can present the chemical and physical environments that cells need to develop properly. (livescience.com)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
She has been reviewer for international Journals ( FASEB J , Cell & Tissue Res , Toxicon, Exp Cell Res ), for national grant agencies (MIUR) and international Foundations (AFM, Association Francaise contre les Myopathies). (units.it)
With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
Muscle injury results in quick activation of the innate immune system which exerts pleiotropic effects on regenerating muscle mass (Brunelli and Rovere-Querini 2008 Tidball and Villalta 2010 Within minutes of injury neutrophils infiltrate hurt skeletal muscle mass and launch tissue-damaging reactive molecules which exacerbate muscle mass damage (Tidball 1995 This initial burst of security damage caused by the innate immune system is followed by a wave of reparative macrophages. (scienceexhibitions.org)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
Erythropoietin receptor expression in nonhematopoietic tissue, including skeletal muscle progenitor cells, raises the possibility of a role for erythropoietin beyond erythropoiesis. (uzh.ch)
Mice with erythropoietin receptor restricted to hematopoietic tissue were used to assess contributions of endogenous erythropoietin to promote skeletal myoblast proliferation and survival and wound healing in a mouse model of cardiotoxin induced muscle injury. (uzh.ch)
Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
In regenerative biology, the overarching goal is to rebuild degenerating or absent tissue with a patient's own cells. (uconn.edu)
Here, we focus on identifying and characterizing tissue resident progenitors. (uconn.edu)
In this regard, we have concentrated on the tissue resident adult progenitors in skeletal muscle and specifically, their role in soft tissue associated heterotopic ossification. (uconn.edu)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
2011). Satellite cells are located in a distinct segment between a layer of connective tissue (basal lamina) and muscle fibers (myofibers). (sciencetrend.ca)
To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. (biomedcentral.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
In our experiments we also study cell and tissue properties (nerve and muscle) that are important in generating the behavioral characteristics observed. (ucla.edu)

a reverse function mutant Talniflumate of Nkx3.2 blocks the ability of Sox9 to both inhibit myogenesis and induce chondrogenesis suggesting that Nkx3.2 is required for Sox9 to promote chondrogenic differentiation in satellite cells. (healthyguide.info)
In vitro culture systems that structurally model human myogenesis and promote PAX7+ myogenic progenitor maturation have not been established. (bvsalud.org)
Culture conditions instigate neural lineage arrest and promote fetal hypaxial myogenesis toward limb axial anatomical identity, with generation of sustainable uncommitted PAX7 myogenic progenitors and fibroadipogenic (PDGFRa+) progenitor populations equivalent to those from the second trimester of human gestation. (bvsalud.org)
In more detail, the team is studying the role of electrical activity and local trophic factors in the microenvironment of satellite cell niche and in the post-mitotic myogenesis. (units.it)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)

These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
Better understanding of the cellular and molecular pathophysiological mechanisms regulating adipocyte size, number and depot-dependent expansion has become a focus of interest over recent decades. (springer.com)
Dr. Bosinger is defining the molecular mechanisms of virus pathogenesis through application of next-gen RNA sequencing to studies of HIV and SARS-CoV-2 infection in non-human primates. (emory.edu)
Comparing and contrasting the molecular mechanisms controlling BAT growth and development in mice and humans will increase our understanding or how human BAT is developed and may identify potential therapeutic targets to increase EE. (biomedcentral.com)
MicroRNAs are molecular mechanisms involved in mouse BAT development however, little is known about the miRNA profile in human BAT. (biomedcentral.com)
Cytokines and growth factors were optimised using the SDE estimator and the mechanisms that a crucial to skeletal muscle repair process. (mister-finch.com)

Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
Intro Satellite cells are the cells specific stem cells in the adult skeletal muscle mass. (healthyguide.info)
Single-cell comparison to human fetal and adult myogenic progenitor /satellite cells reveals distinct molecular signatures for non-dividing myogenic progenitors in activated (CD44High/CD98+/MYOD1+) and dormant (PAX7High/FBN1High/SPRY1High) states. (bvsalud.org)
This can be achieved using induced pluripotent stem cells (iPSCs), human adult cells that have been 'reprogrammed' to behave like cells in the embryo that can develop in to almost any cell in the body. (bvsalud.org)
There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)

One such alternative cell fate is the adipogenic fate as Pax7(+) satellite cells isolated from solitary myofibers used adipogenic fate in addition ARHGAP1 to muscle mass fate significance of these factors we used a mouse fracture healing model inside a genetically altered reporter mouse where muscle mass progenitors were lineage-traced. (healthyguide.info)
Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Validation of PAX7 Reporter Lines from Human iPS Cells Using CRISPR/Cas9 Technology. (uh.edu)
Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)
To compare transcriptome profile of PAX7/MYF5 double reporter hESC derived myogenic cells to human skeletal myoblasts, we enlisted ENCODE polyA RNA-Seq data of the human skeletal muscle myoblasts from the Wold lab (GEO: GSM958744). (nih.gov)
This phenotype was associated with a higher number of Pax7 positive cells in mdxθ−/− muscle compared with mdx muscle, during the progression of the disease. (unicatt.it)
The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)

When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
Compared with wild-type controls, these mice had fewer skeletal muscle Pax-7(+) satellite cells and myoblasts that do not proliferate in culture, were more susceptible to skeletal muscle injury and reduced maximum load tolerated by isolated muscle. (uzh.ch)
In contrast, mice with chronic elevated circulating erythropoietin had more Pax-7(+) satellite cells and myoblasts with increased proliferation and survival in culture, decreased muscle injury, and accelerated recovery of maximum load tolerated by isolated muscle. (uzh.ch)
Skeletal muscle myoblasts also produced endogenous erythropoietin that increased at low O(2). (uzh.ch)
We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)

When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
Intro The regenerative response of skeletal muscle mass to ICAM4 injury is dependent within the quiescent populace of skeletal muscle mass stem cells termed the satellite cells which reside beneath the basal lamina of each myofiber (Brack and Rando 2012 Wang and Rudnicki 2012 Upon injury these quiescent satellite cells become triggered and undergo proliferation providing rise to myogenic progenitors (MPs) that ultimately differentiate into mature myofibers. (scienceexhibitions.org)
In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
Methods in Molecular Biology. (uh.edu)
BACKGROUND AND AIMS: The highly heterogeneous cellular and molecular makeup of pancreatic ductal adenocarcinoma (PDAC) not only fosters exceptionally aggressive tumor biology but contradicts the current concept of one size fits all therapeutic strategies to combat PDAC. (bvsalud.org)
Her primary areas of investigation include Cell biology, Immunology, Genetic enhancement, Haematopoiesis and Virology. (research.com)
Her Cell biology research is multidisciplinary, relying on both Dendritic cell, Interferon gamma, Interleukin 12, Bone marrow and Transplantation. (research.com)
The Immunology study combines topics in areas such as Progenitor cell and Molecular biology. (research.com)
Her studies deal with areas such as Cellular differentiation, CD40, Antigen-presenting cell, Dendritic cell and Interleukin 12 as well as Cell biology. (research.com)
Her biological study spans a wide range of topics, including Progenitor cell, CD34, Endothelial stem cell and Molecular biology. (research.com)
Genetic enhancement, Cell biology, Haematopoiesis, Viral vector and Transduction are her primary areas of study. (research.com)
Her Cell biology research is multidisciplinary, incorporating elements of Gene transfer, Transgene, Dose dependence and Immune system. (research.com)
The study incorporates disciplines such as Cell culture, Progenitor cell, CD34, Molecular biology and Transduction in addition to Haematopoiesis. (research.com)
She combines subjects such as Myeloid, Kidney disease and Cell biology with her study of Viral vector. (research.com)
The most prominent example is the changes in cell biology and morphology when it evolves from a healthy to a cancerous state [1, 3]. (globaltechbiz.com)
He then moved to the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany where he performed his postdoctoral studies in the lab of Dr. Thomas Graf from 1991 to 1996. (ubc.ca)
Dr. Jason Doles (pronouns He/Him/His) is an Associate Professor in the Department of Biochemistry and Molecular Biology at Mayo Clinic. (jove.com)
Written in the highly successful Methods in Molecular Biology™ , chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. (dattanibookagency.in)
2009 potentially reflecting the reduced secretion of myogenic growth element IGF-1 by these cells (Wynes and Riches 2003 While these studies demonstrate a facilitative part of innate immune cells in muscle mass regrowth after injury a direct molecular link between the innate immune system and muscle mass progenitor biology remains to be founded. (scienceexhibitions.org)
While this ultimate aim is rather ambitious, the motivation to achieve these ideal clinical tissues has driven the field of stem and progenitor cell biology to unprecedented levels of scientific and public involvement. (uconn.edu)
Ultimately, construction of molecular motors to arbitrary specifications will provide a powerful toolkit for synthetic biology, therapeutics, and nanotechnology. (nih.gov)

The concepts of her Cancer research study are interwoven with issues in Bone marrow failure, FANCA, Stem cell, Bone marrow and In vivo. (research.com)
In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
In vivo studies using transgene/reporter-free hESC/iPSCs further validated myogenic potential of the cells by dystrophin restoration and satellite cell engraftment in NSG-mdx4cv mice. (nih.gov)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
Instead, we identify a novel mesenchymal cell type located in the interstitial space of skeletal muscle that harnesses robust in vivo chondrogenic, osteogenic and adipogenic capabilities. (uconn.edu)
Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
Novel molecular motors will also have ex vivo applications including molecular sorting and assembly of nanoelectronics in microfabricated devices. (nih.gov)
One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
We therefore propose (3) to isolate ovarian tumor stem cells and grow them in vivo using the ESC ovarian cancer model. (nih.gov)

To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
Furthermore, we decisively prove through in vitro clonal assays that the majority of these mesenchymal progenitors are capable of multilineage differentiation, contributing to spontaneously forming adipogenic and fibrocyte-like cells, and to BMP-induced osteogenic cells. (uconn.edu)
Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)

The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)

The Q+ and Q- isoforms of PAX3 are generally co-expressed in cells. (wikipedia.org)
During development, one of the major lineages expressing Pax3 is the skeletal muscle lineage. (wikipedia.org)
Finally we found that in an mouse model of fracture healing where muscle mass progenitor cells were lineage-traced Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle mass progenitor cells that give rise to chondrocytes during fracture restoration. (healthyguide.info)
Therefore our and analyses suggest that the balance of Pax3 Nkx3.2 and Sox9 may act as a molecular Talniflumate switch during the chondrogenic differentiation of muscle mass progenitor cells which may be important for fracture healing. (healthyguide.info)
We found that in the descendents of muscle mass progenitors that contributed to cartilage formation Nkx3.2 and Sox9 were strongly induced while Pax3 manifestation was strongly repressed. (healthyguide.info)
Collectively our data suggest that the balance of Nkx3.2 Sox9 and Pax3 can act as a molecular switch during the chondrogenic differentiation of satellite cells which may play an important part in the healing process experiments were normalized to GAPDH. (healthyguide.info)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)

Cytoskeleton, U-93631 which is a network of interconnected filaments of different types, connects the cell membrane with underlying sub-cellular components. (globaltechbiz.com)
The nucleus is the largest organelle among sub-cellular components, demonstrating solid-elastic behavior [4], and it is typically stiffer than the cell itself [5]. (globaltechbiz.com)
These primitive-like cells hold promise as an autologous cell source for regenerative treatments and importantly, afford us the opportunity to begin to understand seemingly normal cellular processes in pathological contexts. (uconn.edu)
We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)

Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
Taken together, these data indicate that PRDM16 specifies the brown fat lineage from a progenitor that expresses myoblast markers and is not involved in white adipogenesis. (bioseek.eu)

6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)
Two groups of resident progenitors, the skeletal muscle satellite cells (SCs) and vascular endothelium (VE)/vascular endothelial progenitors (VEPs), have been considered to be putative cell sources for ectopic lesions of heterotopic ossification due to their reported multipotent characteristics. (uconn.edu)

The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
2008 For instance autocrine Notch signaling regulates the activation and proliferation of satellite cells (Bjornson et al. (scienceexhibitions.org)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
Osathanon T, Bespinyowong K, Arksornnukit M, Takahashi H, Pavasant P. "Human osteoblast-like cell spreading and proliferation on Ti-6Al-7Nb surfaces of varying roughness. (sciepub.com)
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)
Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)

Cell Stem Cell 2013;13(5):590-601. (scielo.br)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
Stem Cell Res. (uh.edu)
Wu J, Hunt SD, Matthias N, Servián-Morilla E, Lo J, Jafar-Nejad H, Paradas C, Darabi R . Generation of an induced pluripotent stem cell line (CSCRMi001-A) from a patient with a new type of limb-girdle muscular dystrophy (LGMD) due to a missense mutation in POGLUT1 (Rumi). (uh.edu)
Stem Cell Research. (uh.edu)
Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
Here we report that human skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm and neuromesodermal progenitors and develop into organized structures reassembling neural plate border and dermomyotome. (bvsalud.org)
The various areas that Anne Galy examines in her Stem cell study include Fanconi anemia, Bone marrow and Lymphocyte. (research.com)
Her research in Genetic enhancement intersects with topics in Hematopoietic stem cell transplantation, Cancer research, Wiskott-Aldrich syndrome and Prospective cohort study. (research.com)
She interconnects Cell, Progenitor cell, Protein domain, Enhancer and Hematopoietic stem cell in the investigation of issues within Transduction. (research.com)
Anne Galy mainly focuses on Genetic enhancement, Haematopoiesis, Viral vector, Cancer research and Hematopoietic stem cell transplantation. (research.com)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
He is a member of the Stem Cell Network Centre of Excellence (past member of the Stem Cell Policy Committee and Research Management Committee and current Sub-chair of the Training and Education Committee), and a member of the AllerGen Network Centre of Excellence (Research Management Committee and Co-Chair of the Biomarkers Program). (ubc.ca)
Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
In lab analysis, they're used to transform specialized cells, like skin cells, into extra stem-cell-like cells that are pluripotent, suggesting they have the ability to become many various kinds of cells. (sciencetrend.ca)
In this research, the team used two completely different mouse models to pinpoint the muscle stem-cell-specific or niche-specific modifications following the addition of Yamanaka factors. (sciencetrend.ca)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
We hypothesize that the ESC ovarian tumor model, with human vascular cells, will provide an ideal microenvironment to support human stem cell growth. (nih.gov)

During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
2010 FAPs which do not arise from your myogenic lineage are bipotential cells capable of providing rise to fibroblasts and adipocytes. (scienceexhibitions.org)
We use extensively, the Cre/loxP lineage tracing system to label putative plastic cell types and assess the inherent plasticity of these cells in biologically relevant mouse-chick chimeric models of developmental potency and bioassays of heterotopic ossification. (uconn.edu)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)

Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (sciepub.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)

Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)

Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
Other key factors would involve electrically stimulating and mechanically stretching the muscles to exercise them, helping them mature properly, and perhaps growing other cells alongside the satellite cells to provide necessary molecular cues. (livescience.com)
Humans contains around 650 skeletal muscles which allow the body to move around and maintain its posture. (bvsalud.org)
Skeletal muscles are made up of individual cells that bundle together into highly organized structures. (bvsalud.org)
One way to better understand how skeletal muscles are formed, and how this process can go wrong, is to grow them in the laboratory. (bvsalud.org)
Primary MuSCs isolated from individual skeletal muscles (gastrocnemius and masseter) are lysed, and their RNA is reverse-transcribed into cDNA and copied into double-stranded DNA. (biomed.news)
Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)

Human ES and iPS-Derived Myogenic Progenitors Restore Dystrophin and Improve Contractility upon Transplantation in Dystrophic Mice. (uh.edu)
Overall, our results suggest that lack of PKCθ promotes muscle repair in dystrophic mice, supporting stem cells survival and maintenance through increased Delta-Notch signaling. (unicatt.it)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)

In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)
If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)

Furthermore, strategies utilised to improve migratory capacity of myogenic cells are examined in order to identify potential treatments that may be applied to future transplantation protocols. (crick.ac.uk)
Muscle samples were collected and myogenic cells were isolated and cultivated. (nature.com)

Successful engraftment of gene-corrected hematopoietic stem cells in non-conditioned patients with Fanconi anemia. (research.com)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
How are hematopoietic stem cells born during the development of the vertebrate embryo? (uctv.tv)

The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)
In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
These analyses consist of enzyme activities of single muscle or neural cells, cell morphology, the kinds of proteins synthesized, the modulations of the mRNA's of specific myonuclei as well as the physiological properties of the nerve and muscle cells. (ucla.edu)
Myokines are peptides or proteins released from skeletal muscle and are involved in multiple physiological processes, including metabolism and hypertrophy in the autocrine and paracrine manner [ 6 ]. (biomedcentral.com)

The main interest of her research activity is the nerve-muscle communication and the skeletal muscle physiology, including muscle plasticity and impact of ageing on the neuromuscular system. (units.it)
The results of the proposed research will advance the understanding of important aspects of the neuromuscular physiology such as the modulation of the neuromuscular transmission and the skeletal muscle plasticity. (units.it)

Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)

Additionally, safe and efficient migration of transplanted cells is critical in cell therapies, both allogeneic and autologous. (crick.ac.uk)
One of the key reasons for this negative outcome is the limited migration of donor cells, which hinders the overall cell engraftment potential. (crick.ac.uk)
Commensurate with the known truth that HuR improved the great quantity of adhesion substances VLA-4 on Th17 cells, knockout of HuR impaired splenic Th17 cell migration towards the central anxious program and abolished the condition. (globaltechbiz.com)
Molecular motors lie at the heart of biological processes from DNA replication to cell migration. (nih.gov)

Cell Metabolism 2013;17(2):210-24. (scielo.br)
Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
Cell Metabolism, ePub. (edu.au)

We use in parallel many technologies that have been considerably refined to identify, isolate, and characterize, specific and practically pure cell populations, critically allowing us to attribute functional outcomes of potency tests to unique cell types. (uconn.edu)

European Blot analysis For European Blot analysis total protein lysates were obtained following a standard protocol from confluent 6 cm cells culture plates comprising roughly 3×106 cells [25]. (healthyguide.info)
One is, how, and to what extent, does the nervous system control protein expression in skeletal muscle fibers? (ucla.edu)

These cells lay beneath the basement membrane of the muscle mass fiber and are usually mitotically quiescent [1]. (healthyguide.info)
For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)

Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)

In the beginning satellite cells were considered to Talniflumate be unipotent stem cells with the ability of generating a unique specialized phenotype the skeletal muscle mass cells. (healthyguide.info)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)

1 x 106 cells were harvested / sorted at each time- point and RNA extracted using QIAGEN miRNeasy RNA isolation kit (217004, QIAGEN) according to manufacturer's protocol. (nih.gov)

Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)

Erythropoietin acts by binding to its cell surface receptor on erythroid progenitor cells to stimulate erythrocyte production. (uzh.ch)

The formation of skeletal muscle:from somite to limb. (scielo.br)
Skeletal muscle MRI studies in limb-girdle muscular dystrophy (LGMD) have increased over the past decades, improving the utility of MRI as a differential diagnostic tool. (mdpi.com)
7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)

In conclusion, these data decisively establish degrees of potency for skeletal muscle resident progenitors and identify the putative cell-of-origin for the ectopic skeletal anlagen of heterotopic ossification. (uconn.edu)

Finally, our preliminary data suggest that the ESC-ovarian tumor model has human tumor vascular cells. (nih.gov)
Tumor vascular cells are critical for the growth of tumor stem cells, which reside within the vascular niche. (nih.gov)

abstract = "Duchenne muscular dystrophy (DMD) is a genetic disease characterized by muscle wasting and chronic inflammation, leading to impaired satellite cells (SCs) function and exhaustion of their regenerative capacity. (unicatt.it)

These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)

Skeletal muscle is characterized by a remarkable capacity to rearrange after physiological changes and efficiently regenerate. (biomed.news)

However, a huge number of autologous cells are required to address these practices. (sciepub.com)

They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
Substrate diffusion is the constant movement of substrate molecules caused by molecular motion. (jove.com)

Upon injury or when challenged with a variety of mechanical or biochemical stimuli satellite cells re-enter the cell cycle and give rise to Talniflumate differentiated myocytes which form new muscle mass materials or fuse with existing materials and contribute to muscle mass growth and restoration [1]. (healthyguide.info)

Anatomy54

Organisms20

Diseases8

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes39

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science5

Health Care2

Regulating skeletal muscle1

Regeneration39

Embryonic9

Tissue24

Myogenesis8

Mechanisms7

Adult10

Pax76

Myoblasts7

Myofibers6

Biology19

Vivo10

Vitro5

Tissues5

Pax37

Cellular4

Myoblast2

Endothelial2

Proliferation7

Stem Cell24

Lineage4

Bone marrow3

Isoforms1

Muscles7

Mice3

Murine4

Myogenic cells2

Hematopoietic stem3

Proteins6

Plasticity2

Differentiate2

Migration4

Metabolism3

Populations1

Protein2

Quiescent2

Dystrophin1

Transplantation1

Phenotype1

Adipose1

Isolation1

Engraftment4

Surface receptor1

Limb4

Anlagen1

Vascular2

ABSTRACT1

Ultimately1

Physiological1

Autologous1

Molecules3

Myocytes1