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In Situ HybridizationIn Situ Hybridization, FluorescenceMass SpectrometryNucleic Acid HybridizationFluorescenceSpectrometry, FluorescenceSpectrometry, Mass, Electrospray IonizationMicroscopy, FluorescenceTandem Mass SpectrometryMolecular Sequence DataRNA, MessengerGas Chromatography-Mass SpectrometrySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationFluorescence PolarizationBase SequenceDNA ProbesFluorescent DyesAmino Acid SequenceRNA ProbesHybridization, GeneticImmunohistochemistryFluorescence Resonance Energy TransferPolymerase Chain ReactionCloning, MolecularChromatography, LiquidChromosome AberrationsGene ExpressionDigoxigeninOligonucleotide ProbesChromosome MappingBlotting, NorthernDNAGene AmplificationKaryotypingChromatography, High Pressure LiquidDNA, ComplementaryComparative Genomic HybridizationSensitivity and SpecificityTranslocation, GeneticSequence Analysis, DNAGene Expression Regulation, DevelopmentalChromosome BandingSpectrometry, Mass, Secondary IonTissue DistributionAneuploidyPhylogenyRNA, Ribosomal, 16SReverse Transcriptase Polymerase Chain ReactionKineticsReproducibility of ResultsDNA, BacterialSequence Homology, Amino AcidBlotting, SouthernCarcinoma in SituTime FactorsDNA, RibosomalFluorescence Recovery After PhotobleachingProteomicsDNA PrimersGene DosageMicroscopy, Fluorescence, MultiphotonOligonucleotide Array Sequence AnalysisSpecies SpecificityTranscription, GeneticCell LineGreen Fluorescent ProteinsSpectrometry, Mass, Fast Atom BombardmentCells, CulturedPrimed In Situ LabelingProtein BindingChromosomes, Human, Pair 1Chromosomes, Human, Pair 17Nucleic Acid ProbesMicroscopy, ConfocalParaffin EmbeddingGene Expression ProfilingDNA, ViralChromosomes, Human, Pair 7ProteinsMolecular Probe TechniquesGene Expression RegulationRNAMutationGene LibraryRats, Sprague-DawleyCytogenetic AnalysisOrgan SpecificityCattleTryptophanLuminescent ProteinsPeptide Nucleic AcidsBrainChromosomes, Human, Pair 11Chromosome DeletionRhodaminesPeptidesCarbocyaninesSequence AlignmentStaining and LabelingInterphase

Mass spectrometryImmunohistochemistrySequencingFISHDetectionPermeabilityTissueMolecularAnalysisCellScienceSamplesHybridMassMicroarrayMicroscopyNucleicMicroscopeCellsDiagnosisGeneticAmplificationCapableSectionsProcessResultsLargeKits

Mass spectrometry18

• Mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. (wikipedia.org)
• We performed microbiologic analysis of all biofilm samples and moth flies by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (Bruker, https://www.bruker.com ) and Phoenix automated microbiology system (Becton Dickinson, https://www.bd.com ) according to European Committee on Antimicrobial Susceptibility Testing (EUCAST, https://eucast.org ) guidelines. (cdc.gov)
• I am also familiar with lipid analysis and nanoscale secondary ion mass spectrometry (nanoSIMS). (stanford.edu)
• We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of N-15-enriched diazotrophic Vibrio sp. (ifremer.fr)
• Bioinformatic analysis, RNA-protein pull down, mass spectrometry, RNA immunoprecipitation, luciferase reporter assay and fluorescence in situ hybridization were performed to validate the HNRNPL/circFAM13B/IGF2BP1/PKM2 cascade. (biomedcentral.com)
• MLSB and tetracycline antimicrobials were quantified after solid-phase extraction using liquid chromatography-tandem mass spectrometry. (illinois.edu)
• Catalyzed reporter deposition-fluorescence in-situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (nanoSIMS) show that W. gerlachensis preferentially assimilates xylose. (uibk.ac.at)
• Immunocoprecipitation-mass spectrometry (IP-MS), proteomic sequencing and Co-immunoprecipitation (CO-IP) were used to identify and examine the interaction between the NCAPG and CKII proteins. (biomedcentral.com)
• By combining stable isotope probing (SIP), fluorescence in situ hybridization (FISH), scanning electron microscopy (SEM), confocal Raman microspectroscopy (Raman), and nano-scale secondary ion mass spectrometry (NanoSIMS), we illustrate how individual cells can be thoroughly interrogated to obtain information about their taxonomic identity, structure, physiology, and metabolic activity. (montana.edu)
• Using metagenome sequencing, single-gene fluorescence in situ hybridization, immunohistochemistry, shipboard incubations and in situ mass spectrometry, we show here that the symbionts of the hydrothermal vent mussel Bathymodiolus from the Mid-Atlantic Ridge use hydrogen to power primary production. (eudat.eu)
• The regulatory mechanism of lncSNHG5 was investigated by RNA sequencing, fluorescent in situ hybridization, cellular fractionation assay, mass spectrometry, RNA pull-down, RNA immunoprecipitation, gene-specific m6A assay, chromatin immunoprecipitation, dual luciferase reporter assay and actinomycin D treatment in CAFs and NFs. (thno.org)
• Biochemical approaches such as proteomics and mass spectrometry and biomechanical concepts will also be discussed. (cshl.edu)
• A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
• PURPOSE: Tandem mass spectrometry (MS/MS) is effective screening test for inherited metabolic diseases. (koreamed.org)
• In this study, we estimate potential costs and benefits of using tandem mass spectrometry (MS/MS) to screen. (koreamed.org)
• Welcome to our comprehensive guide on Mass Spectrometry, a powerful analytical technique that has revolutionized the scientific world. (acebioscience.com)
• The system is a highly sensitive, cost-effective, mass spectrometry-based platform for high-throughput genetic analysis, and is used globally in diverse research fields such as cancer profiling for solid tumors and liquid biopsies, inherited genetic disease testing, pharmacogenetics, agricultural genomics, and clinical research. (agenabio.com)
• Indeed, while methods of genetic testing such as in situ hybridization or detection tests such as protein mass spectrometry are widely used in the molecular diagnostic area, polymerase chain reaction remains one of the most fundamental techniques used to amplify specific sequences of DNA or RNA in molecular diagnostic tests [7]. (symbiosisonlinepublishing.com)

Immunohistochemistry1

• Additional methods include mRNA in situ hybridization and protein immunohistochemistry as well as basic bioinformatic techniques for gene comparison and functional analysis. (cshl.edu)

Sequencing3

• In situ sequencing and fluorescence in situ hybridization (FISH) do not require that cells be isolated and are increasingly being used for analysis of tissues. (wikipedia.org)
• Bone-metastatic circRNAs were screened using circRNAs deep sequencing and validated using in situ hybridization in BC tissues with or without bone metastasis. (biomedcentral.com)
• Moreover, there is an increasing adoption rate of in situ sequencing techniques, which would further boost the segment's growth. (grandviewresearch.com)

FISH6

• I combine fieldwork and in-situ experiments with laboratory analyses and apply cutting-edge geochemical and molecular biological techniques, including isotopic tracers, DNA and RNA analysis, gene tree/species tree reconciliation and ancestral character state reconstruction, fluorescence in-situ hybridization (FISH), and microbiological culturing. (stanford.edu)
• Bley N.: Immunofluorescence, FRAP analyses and fluorescence in situ hybridization (FISH) experiments. (forschung-sachsen-anhalt.de)
• The effect of NCAPG on the proliferation of HCC cell lines was evaluated using an EdU incorporation assay, a Cell Counting Kit-8 assay and Fluorescence in situ hybridization (FISH). (biomedcentral.com)
• PURPOSE: The purpose of this study was to evaluate the clinical utility of rapid detection of Down syndrome and Edward syndrome by Interphase Fluorescence in Situ Hybridization (FISH) analysis METHODS: A. (koreamed.org)
• Fluorescence In Situ Hybridization (FISH) is a powerful and revolutionary molecular biology technique that has transformed our understanding of cellular genetics. (acebioscience.com)
• While Fluorescence In Situ Hybridization (FISH) is the gold standard for gene fusion detection due to its high sensitivity and specificity, it is also labor intensive, subjective in analysis, and unable to screen a larger number of gene fusions. (agenabio.com)

Detection1

• Integrated Genomic DNA/RNA Profiling vs Fluorescence in Situ Hybridization in the Detection of MYC and BCL2 (and BCL6) Rearrangements in Large B-Cell Lymphomas: Updates Amid the New WHO Classification of Lymphoid Neoplasms. (cdc.gov)

Permeability1

• In vitro permeability assays, trans-endothelial invasion assays, in vivo permeability assays and tissue fluorescence staining were conducted to examine vascular permeability. (thno.org)

Tissue1

• Tubule formation and three-dimensional sprouting assays and tissue fluorescence staining were conducted to investigate angiogenesis. (thno.org)

Molecular3

• The fluorescence intensities indicate that the molecular beacon exists either as a hybrid with its perfect complementary target sequence, exhibiting high selleck kinase inhibitor fluorescence from 25°C to 55°C, or in its free state in the form of a stem-loop structure with fluorescence quenched in a temperature range of 25-65oC as depicted by the cartoons. (pkc-inhibitors.com)
• At higher temperatures (more than 70oC) the molecular beacon probe denatures and exhibits high fluorescence intensities in control. (pkc-inhibitors.com)
• Fludarabine order APH1387 and ACTA1 molecular beacon probes depicted a temperature and fluorescence profile (B, C, and D), which is similar to the RecA3 molecular beacon probe. (pkc-inhibitors.com)

Analysis1

• Evaluation of F1 and BC1 hybrids of Lilium lancifolium × Asiatic hybrid 'Chianti' by morphological analysis and fluorescence in situ hybridization. (livedna.net)

Cell1

• The power of Xenopus can be leveraged when live-cell fluorescence imaging is combined with microsurgery, grafting, and dissociated cell culture. (cshl.edu)

Science1

• Application of genomic in situ hybridization in horticultural science. (livedna.net)

Samples2

• To visualize biofilms we used fluorescence in situ hybridization protocol on sections of embedded samples or moth flies ( Appendix ). (cdc.gov)
• The prevalence of the ribosomal modification responsible for MLSB resistance in the same samples was quantified using fluorescence in situ hybridization. (illinois.edu)

Hybrid1

• Similarly, probe-target hybrid also denatures at higher temperature releasing the target and diminishing the fluorescence as the probe returns to hairpin-loop structure. (pkc-inhibitors.com)

Mass12

• Mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. (wikipedia.org)
• We performed microbiologic analysis of all biofilm samples and moth flies by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (Bruker, https://www.bruker.com ) and Phoenix automated microbiology system (Becton Dickinson, https://www.bd.com ) according to European Committee on Antimicrobial Susceptibility Testing (EUCAST, https://eucast.org ) guidelines. (cdc.gov)
• 9. Histopathology-guided mass spectrometry differentiates benign nevi from malignant melanoma. (nih.gov)
• 16. Proteomic Mass Spectrometry Imaging for Skin Cancer Diagnosis. (nih.gov)
• Access to gas Chromatography - Mass Spectrometry (GC-MS) for determination of short fatty acids or scanning electron microscope SEM ZEISS DSM 950 for ultrastructure analysis. (europlanet-society.org)
• Next, we used two-dimensional polyacrylamide gel electrophoresis and mass spectrometry to identify 42 proteins that were overexpressed in the cancer cells relative to normal cells. (nih.gov)
• Development and validation of a 2nd tier test for identification of purine nucleoside phosphorylase deficiency patients during expanded newborn screening by liquid chromatography-tandem mass spectrometry. (medscape.com)
• In this project we seek a motivated individual, with extensive mass spectrometry experience, who wants to develop a new instrument and apply it to the study of MDa. (vacancyedu.com)
• You will be based in the team of Prof. Perdita Barran in the Michael Barber Centre for Collaborative Mass Spectrometry, and work closely with Dr. John Hoyes (TrueMass Ltd.), Dr. Dimitris Papamastasiou (Fasmatech Ltd.) and Prof. Rob Appleby (UoM). (vacancyedu.com)
• We propose to compare the capabilities of this instrument with that of commercially available mass spectrometry platforms and other methods. (vacancyedu.com)
• Burgt, Y.E.M. van der & Mesker, W.E. (2023), Development of Tier 2 LC-MRM-MS protein quantification methods for liquid biopsies, JOURNAL OF MASS SPECTROMETRY AND ADVANCES IN THE CLINICAL LAB 27: 49-55. (universiteitleiden.nl)
• While a number of reviews on the diagnosis of sepsis have recently been published we here present up-to-date new developments including multiplex PCR, mass spectrometry and array techniques. (akjournals.com)

Microarray1

• When a bacterial sample's DNA is spread over the microarray, specific hybridization to these probes can help in bacterial identification and characterization. (universe84a.com)

Microscopy1

• Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes. (iiab.me)

Nucleic3

• [9] The hybridization signals for each probe when a nucleic abnormality is detected. (iiab.me)
• In-situ hybridization is used for localization of nucleic acids and specific genes in cells, tissues, and histological sections. (emergenresearch.com)
• Increasing applications of in-situ hybridization kits in pre-hybridization, post-hybridization, and hybridization treatments and use of kits in research laboratories for specific nucleic acid investigations in cells and tissues is driving revenue growth of this segment. (emergenresearch.com)

Microscope2

• At the end of the assay the tissue samples are visualized under a fluorescence microscope. (iiab.me)
• By observing the cells under a fluorescence microscope, one can identify bacterial species or groups. (universe84a.com)

Cells1

• Mammalian cell biology is dynamic, but today's 'omic approaches require destruction of cells to access their molecular contents and cannot resolve the full richness of cellular diversity in situ . (nih.gov)

Diagnosis1

• The cancer diagnosis segment accounted for largest revenue share in the global in-situ hybridization market in 2021. (emergenresearch.com)

Genetic1

• Rising use of fluorescence ISH for genetic anomalies and gene rearrangements is expected to drive revenue growth of this segment between 2022 and 2030. (emergenresearch.com)

Amplification2

• Signal amplification is achieved via series of sequential hybridization steps. (iiab.me)
• The LungFUSION Panel runs on the MassARRAY System, and requires an initial cDNA synthesis step, followed by two rounds of amplification, and spectrometry. (akampion.com)

Capable2

• Capable of producing a new moiety that generates fluorescence. (nationalacademies.org)
• The device is capable of performing both fluorescence and chromogenic ISH, thus opening up a wide range of applications of product in industries as well as research sectors. (emergenresearch.com)

Sections2

• In situ hybridization of rat whole brain sections showed an mRNA expression pattern similar to that observed for human P450 20A1, indicating expression in substantia nigra, hippocampus, and amygdala. (nih.gov)
• To visualize biofilms we used fluorescence in situ hybridization protocol on sections of embedded samples or moth flies ( Appendix ). (cdc.gov)

Process1

• The probe must be large enough to hybridize specifically with its target but not so large as to impede the hybridization process. (iiab.me)

Results1

• Fluorescence in situ hybridization images and metagenome results suggest that Methanobacterium spp. (asu.edu)

Large1

• The fluorescence in-situ hybridization segment accounted for a significantly large revenue share in 2021. (emergenresearch.com)

Kits1

• Demand for staining solutions blockers, and hybridization and pre-hybridization buffers is increasing with increasing usage of in-situ hybridization kits. (emergenresearch.com)