• Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity. (wikipedia.org)
  • Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes. (wikipedia.org)
  • Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
  • In the fall of 2011 Dr. Sharakhov's laboratory installed an Xmatrx Infinity system to physically map chromosomes of various invertebrate species using automated fluorescent in situ hybridization (FISH). (biogenex.com)
  • Gole L.A., Bongso A., Fluorescent in-situ hybridization - Some of its applications in clinical cytogenetics, Singapore Med. (gse-journal.org)
  • The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)
  • Physical mapping of ribosomal genes by Fluorescent Molecular in situ Hybridization. (tsukuba.ac.jp)
  • fluorescent in situ hybridisation (FISH) using five different repetitive DNA-probes showed a pericentric inversion of 1R in both lines. (unboundmedicine.com)
  • and fluorescent in, situ hybridization (FISH) to metaphase chromosomes revealed an insertion of part of chromosome 16 on chromosome 11. (lu.se)
  • Fluorescence "in situ" hybridization (FISH) technique enables chromosomes to be tagged with fluorescent dyes to highlight genetic traits. (lu.se)
  • FISH allows the analysis of a large series of archival cases much easier to identify the pinpointed chromosome by creating a probe with an artificial chromosomal foundation that will attract similar chromosomes. (wikipedia.org)
  • In particular, G band analysis usually does not show the chromosomal origin of small marker chromosomes or of a small amount of extra material detected on otherwise normal chromosomes. (bmj.com)
  • Comparative genomic hybridisation (CGH) is one of several molecular approaches that can be applied to ascertain the origin of extra chromosomal material. (bmj.com)
  • Chromosomal identification of marker chromosomes and extra bands on chromosomes through molecular techniques is always of clinical interest. (bmj.com)
  • The overall aim of this project was to develop the technique of chromosomal in situ suppression (CISS) hybridization using whole chromosome specific libraries (chromosome painting) and to apply it to the investigation of diagnostic problems in clinical cytogenetics. (gla.ac.uk)
  • Dr. Sharakhov is focused on identifying the chromosomal physical map. (biogenex.com)
  • Human chromosome 15q25 is involved in several disease-associated structural rearrangements, including microdeletions and chromosomal markers with inverted duplications. (pacb.com)
  • Chromosomal mapping of the bovine aromatase gene (CYP19) and an aromatase pseudogene to Chromosome 10 and syntenic group U5. (fbn-dummerstorf.de)
  • This detailed map demonstrates the power of high-resolution genetic mapping in the mouse as a means of determining locus order in a small chromosomal region and of providing an accurate framework for the construction of physical maps. (mpi.nl)
  • Seven different mildew resistant wheat lines derived from crosses between triticale and bread wheat were examined by molecular cytogenetics and chromosome C-banding in order to determine their chromosomal composition. (unboundmedicine.com)
  • The following chromosomal conditions are associated with changes in the structure or number of copies of chromosome 1. (medlineplus.gov)
  • 1q21.1 microdeletion is a chromosomal change in which a small piece of the long (q) arm of chromosome 1 is deleted in each cell. (medlineplus.gov)
  • [ 1 ] They described a child with midline fusion defects, and subsequent cytogenetic studies revealed a chromosomal deletion of the short arm of chromosome 4. (medscape.com)
  • Comparative genomic hybridisation (CGH) is a dual fluorescence in situ hybridisation protocol capable of detecting global gains and losses of genomic regions by competitive DNA hybridisation. (bmj.com)
  • To investigate the possibility that a novel candidate gene for XLMR was disrupted at the X chromosome translocation breakpoint, we mapped the breakpoint using fluorescence in situ hybridisation (FISH). (bmj.com)
  • We compared the miRNA expression profiles of MM with 1q21 gains and MM with normal fluorescence in situ hybridisation (FISH) by gene expression array. (karger.com)
  • We have also demonstrated specific hybridization of fluorescence-labeled probes to single-copy genomic DNA sequences on the free chromatins. (nih.gov)
  • We have used comparative genomic hybridisation to analyse 19 constitutional chromosome abnormalities detected by G band analysis, including seven deletions, five supernumerary marker chromosomes, two interstitial duplications, and five chromosomes presenting with abnormal terminal banding patterns. (bmj.com)
  • Although the structural chromosome abnormalities in these cases were too small to identify the origin of the extra genomic material based on banding pattern, none of the abnormalities described by these authors can be considered to be subtle or cryptic. (bmj.com)
  • Intriguingly, we found that the X chromosome breakpoint in the daughter could not be defined by a single breakpoint spanning genomic clone and further analysis showed a 650 kb submicroscopic duplication between DXS7067 and DXS7060 on either side of the X chromosome translocation breakpoint. (bmj.com)
  • Xq28 appears to be an unstable region of the human genome and genomic rearrangements are recognised as major causes of two single gene defects, haemophilia A and incontinentia pigmenti, which map within Xq28. (bmj.com)
  • Libert F., Lefort A., Okimoto R., Womack J., Georges M., Construction of a bovine genomic library of large yeast artificial chromosome clones, Genomics 18 (1993) 270-276. (gse-journal.org)
  • By comparative genomic hybridization using DNA from sperm and unfertilized eggs of this coral, we offer evidence suggesting the existence of sex chromosomes in this species. (bioone.org)
  • Genomic in situ hybridisation (GISH) showed the presence of rye germplasm in all the lines and identified three substitution lines, three double substitution lines and one addition-substitution line. (unboundmedicine.com)
  • A detailed mapping of the chromosome abnormality using whole genome array based comparative genomic hybridization (CGH) of the patient DNA revealed a duplication 16q12.1-q22.1 corresponding to gain of 19.8 Mb of DNA without any detectable loss of genetic material on chromosome 11. (lu.se)
  • His current work is focused on two broad goals: (1) mapping variants and genes that confer risk to human disease, with ongoing projects focused on coronary artery disease and cardiometabolic traits in unique and underrepresented populations, and (2) developing methods for the detection and interpretation of human genome variation, with an emphasis on structural variation and other difficult-to-detect forms, and on comprehensive trait association in human disease studies. (yale.edu)
  • Considerable progress has been achieved in Conifer genome mapping by the use of megagametophyte tissue and RAPDs (3). (le.ac.uk)
  • The construction of BAC-based physical maps of individual chromosomes reduces the complexity of this allohexaploid genome, enables elucidation of gene space and evolutionary relationships, provides tools for map-based cloning, and serves as a framework for reference sequencing efforts. (biomedcentral.com)
  • Here, we provide a chromosome-specific view into the organization and evolution of the D genome of bread wheat, in comparison to one of its ancestors, revealing recent genome rearrangements. (biomedcentral.com)
  • Bread wheat ( Triticum aestivum L.) originated from a spontaneous hybridization between the cultivated tetraploid wheat Triticum turgidum L. (2n = 4x = 28, AABB genome) and the wild diploid grass Aegilops tauschii Coss. (biomedcentral.com)
  • In the absence of a finished quality genome sequence, insights into wheat genome structure and function have been accumulating through survey sequencing of individual chromosomes or chromosome-specific Bacterial Artificial Chromosome (BAC) libraries. (biomedcentral.com)
  • Twenty years ago genetic studies were based not on disease but on chromosomes, because it was so hard to study a whole genome. (nih.gov)
  • Segmental Genome Duplication (SGD) - Alterations in chromosome numbers - aneuploidy, autopolyploidy and allopolyploidy. (tsukuba.ac.jp)
  • Albert Levan, a researcher at Lund University, was the first to establish how many chromosomes there are in the human genome. (lu.se)
  • In the 1950s, Albert Levan and American Joe Hin Tjio improved the method, and in 1956 they established that the human genome is divided into 46 chromosomes, i.e. 23 pairs of chromosomes. (lu.se)
  • Fluorescence in situ hybridization to metaphase chromosomes or chromatin fibers in interphase nuclei is a powerful technique in mapping genes and DNA segments to specific chromosome region. (nih.gov)
  • A team of IAE scientists determined the physical location of 242 tammar wallaby genes on their eight pairs of chromosomes. (edu.au)
  • They used a technique that fluorescently tags genes, enabling their location on a chromosome to be visualised. (edu.au)
  • Characterisation of breakpoints in patients with apparently balanced constitutional chromosome rearrangements and phenotypic abnormalities has proved an invaluable strategy for identifying disease causing genes, especially those on the X chromosome. (bmj.com)
  • 12- 17 However, there is still further allelic heterogeneity within this region as there are additional XLMR families published that map to Xq28 with a significant lod score but do not have abnormalities in any of these genes. (bmj.com)
  • Lemieux N., Dutrillaux B., Viegas-Pequignot E., A simple method for simultaneous R- or G-banding and fluorescence in situ hybridization of small single-copy genes, Cytogenet. (gse-journal.org)
  • Prader-Willi and Angelman syndrome (PWS and AS) are complex neurobehavioral disorders associated with loss of function of a cluster of differentially expressed imprinted genes in chromosome 15q11-q13 [ 1 ]. (biomedcentral.com)
  • These findings support the possibility that the high plasticity of these regions, sites of DNA exchange among different chromosomes, could trigger the emergence of new genes. (anthropogeny.org)
  • Identifying genes on each chromosome is an active area of genetic research. (medlineplus.gov)
  • Because researchers use different approaches to predict the number of genes on each chromosome, the estimated number of genes varies. (medlineplus.gov)
  • Chromosome 1 likely contains 2,000 to 2,100 genes that provide instructions for making proteins. (medlineplus.gov)
  • Researchers have identified several possible tumor suppressor genes in the deleted region of chromosome 1, and more research is needed to understand what role these genes play in neuroblastoma development. (medlineplus.gov)
  • Chromosomes have undergone changes in gene arrangement throughout evolution. (edu.au)
  • The team compared this gene organisation to the South American opossum and predicted what the chromosomes of the ancestor of all marsupials would have looked like. (edu.au)
  • The technique was reliable and sensitive and was applied to map the gene for angiotensinogen to 1q42. (gla.ac.uk)
  • 3- 6 Phenotypic abnormalities seen in cases with apparently balanced chromosome rearrangements have usually been explained by the disruption of a gene at the breakpoint causing the loss of gene function. (bmj.com)
  • 18- 21 Xq28 is a highly gene rich region of the human X chromosome and we focused on the X chromosome breakpoint as the identification of a disrupted gene here would be a rapid way to identify a further candidate gene for X linked mental retardation within Xq28. (bmj.com)
  • Initial studies with isozymes (1) indicate that pines exhibit conservation of gene order and this hypothesis will be examined further by the use of transcript based linkage maps. (le.ac.uk)
  • In this study, we constructed the first comprehensive physical map of wheat chromosome arm 5DS, thereby exploring its gene space organization and evolution. (biomedcentral.com)
  • We performed a molecular cytogenetic investigation of the scleractinian coral Acropora solitaryensis, which is dominant in the temperate region of Japan (30-35°N). Molecular cytogenetic analysis, using fluorescence in situ hybridization (FISH), was carried out for karyotyping and gene mapping. (bioone.org)
  • FISH mapping of the rRNA gene was carried out with a probe generated by PCR amplification using rRNA gene primers. (bioone.org)
  • Genetic studies of DNA from families with cystinuria reveal a defective gene located on chromosome 2. (medscape.com)
  • So what we designed a fluorescence in situ hybridization procedure to detect the fusion gene. (nih.gov)
  • The inversion breakpoints map within segmental duplications containing core duplicons of the GOLGA gene family and correspond to the site of an ancestral centromere, which became inactivated about 25 million years ago. (pacb.com)
  • Mapping of the Interferon γ gene (IFNG) to Chromosomes 3 in sheep and 5 in goat by FISH. (fbn-dummerstorf.de)
  • The bovine butyrophilin encoding gene (BTN) maps to Chromosome 23. (fbn-dummerstorf.de)
  • Comparative mapping of the gene coding for Inhibin-α (INHA) to Chromosome 2 in sheep and cattle. (fbn-dummerstorf.de)
  • Mapping of the gene encoding for Inhibin-βA (INHBA) to Chromosome 4q26 in sheep. (fbn-dummerstorf.de)
  • The macrophage expressed variant of the bovine lysozyme-encoding gene maps to Chromosome 5q23. (fbn-dummerstorf.de)
  • Using gene expression, non-coding conserved sequence (NCCS) and synteny data, we have genetically mapped a putative Luzp2 neuronal enhancer responsible for ~33% of allelic transcriptional activity. (biomedcentral.com)
  • The mapping of a putative Chrna7 neuronal enhancer inside the deletion has significant implications for understanding the transcriptional regulation of this schizophrenia-susceptibility candidate gene. (biomedcentral.com)
  • He suggested to introduce the term chromosomics being equal to cytogenomics to bring the three-dimensional morphologically of chromosomes into the focus of research, as this is essential for gene regulation. (frontiersin.org)
  • The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer. (gentaurtop.com)
  • CONCLUSION: Our data indicate that an ancestral gene, originated as a rearranged portion of the primate DDX11 gene, and propagated along many subtelomeric locations, is emerging within subtelomeres of human chromosomes, defining a novel gene family. (anthropogeny.org)
  • The responsible gene has been mapped to band 12q24.1, which encodes the human transcription factor TBX5. (medscape.com)
  • [ 2 ] The defect in the X chromosome is proximal to the gene for factor VIII at Xq28. (medscape.com)
  • Most phenotypic manifestations in this syndrome reflect a contiguous gene syndrome, leading to a phenotypic map of chromosome arm 4p. (medscape.com)
  • Fluorescence-labeled DNA probes constructed from three whole house mouse (Mus domesticus) chromosomes were hybridized to metaphase spreads from deer mouse (Peromyscus maniculatus) to identify homologies between the species. (tamu.edu)
  • We had lots of physical markings of chromosomes to do," said Dr. Sharakhov whose project is now published in the Journal of Visualized Experiments (JoVE): George, P., Sharakhova, M. V., Sharakhov, I. V. High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization. (biogenex.com)
  • Also, due to Xmatrx's ease of use, high-throughput capabilities and proven success, Dr. Sharakhov noted that there were several colleagues who were inquiring with him about collaborations involving high-throughput screening of samples or other projects related to the physical mapping of chromosomes. (biogenex.com)
  • Fluorescence in situ hybridization signals have been detected for sequences separated as close as 21 kilobase pairs and as far as 350 kilobase pairs, with excellent correspondence between the observed and expected distances. (nih.gov)
  • It was developed by biomedical researchers in the early 1980s to detect and localize the presence or absence of specific DNA sequences on chromosomes. (wikipedia.org)
  • CGH has been widely applied to detect gains and losses of DNA sequences on specific chromosomes in the study of solid tumours, 1 but has not been extensively used to study unbalanced constitutional karyotypes. (bmj.com)
  • An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
  • Characterisation and mapping of expressed sequence tags or EST's to serve as anchored sequences tagged sites (STS). (le.ac.uk)
  • Eggen A., Solinas T.S., Fries R.A., Cosmid specific for sequences encoding a microtubule associated protein, MAPIB, contains a polymorphic microsatellite and maps to bovine chromosome 20q14, J. Hered. (gse-journal.org)
  • Generation of chromosome fragment specific DNA sequences by microdissection and DOP-PCR. (fbn-dummerstorf.de)
  • Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. (bvsalud.org)
  • The main part deals with examples of modern FISH-applications, highlighting unique possibilities of the approach, like the possibility to study individual cells and even individual chromosomes. (frontiersin.org)
  • However, the genetic origin and evolution process of this neo-chromosome remains unclear. (geneticsmr.com)
  • Integrating genetic maps and models. (le.ac.uk)
  • The map position of cDNA clones relative to the predicted location of quantitative trait loci will provide new opportunities to unravel the genetic control of polygenic traits in forest tree species. (le.ac.uk)
  • These data have enabled us to construct a high-resolution genetic map for the approximately 3-cM interval between DXWas70 and Cybb on the mouse X chromosome, which encompasses 10 loci. (mpi.nl)
  • Genetic and Cytological Maps. (tsukuba.ac.jp)
  • Genetic mapping approaches in plant and animals. (tsukuba.ac.jp)
  • Aneuploidy applied to genetic mapping. (tsukuba.ac.jp)
  • 1p36 deletion syndrome is caused by a deletion of genetic material from a specific region in the short (p) arm of chromosome 1. (medlineplus.gov)
  • A 1q21.1 microduplication is a copied (duplicated) segment of genetic material at position q21.1 on one of the two copies of chromosome 1 in each cell. (medlineplus.gov)
  • Both the major rDNA (18S rDNA) and the minor rDNA (5S rDNA) were located on the C. semilaevis chromosomes by fluorescence in situ hybridization (FISH). (geneticsmr.com)
  • Furthermore, the telomeres and centromeres of all chromosomes were visualized using FISH. (bioone.org)
  • Comparative mapping of sheep inhibin subunits alpha (INHA) and beta B(INHBB) to chromosome 2 in goat by FISH. (fbn-dummerstorf.de)
  • A short introduction how cytogenetics and molecular cytogenetics were established is followed by technical aspects of fluorescence in situ hybridization (FISH). (frontiersin.org)
  • Here especially highlighted variations of FISH are molecular combing, chromosome orientation-FISH (CO-FISH), telomere-FISH, parental origin determination FISH (POD-FISH), FISH to resolve the nuclear architecture, multicolor-FISH (mFISH) approaches, among other applied in chromoanagenesis studies, Comet-FISH, and CRISPR-mediated FISH-applications. (frontiersin.org)
  • This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
  • The image shows chromosomes from a cancer patient, showing 2:3 translocation. (lu.se)
  • Other changes in chromosome 4 can involve a ring structure or translocation. (medscape.com)
  • Therefore, free chromatin mapping can be generally used to study the structure and organization of mammalian genomes. (nih.gov)
  • General overview of chromosomes behavior during cell cycles - mitosis and meiosis, chromatin strcuture and molecular organization of the chromosomes. (tsukuba.ac.jp)
  • Alterations in chromosome structure - deficiencies/deletions, duplications, inversions and translocations. (tsukuba.ac.jp)
  • Cai L., Taylor J. F., Wing R.A., Gallagher D.S., Woo S.S., Davis S.K., Construction and characterization of a bovine bacterial artifical chromosome library, Genomics 29 (1995) 413-425. (gse-journal.org)
  • Others have applied CGH to the study of constitutional chromosome abnormalities detected postnatally. (bmj.com)
  • We present here the clinical findings and a fine mapping of the associated structural chromosome abnormalities. (lu.se)
  • We were building YAC [yeast artificial chromosome] libraries, cosmid libraries. (nih.gov)
  • We found that two independent inversions occurred in this region after the fission event that gave rise to phylogenetic chromosomes XIV and XV in humans and great apes. (pacb.com)
  • Humans normally have 46 chromosomes in each cell, divided into 23 pairs. (medlineplus.gov)
  • If you look at the karyotype, the chromosome map, you will see they are all different sizes. (nih.gov)
  • An abnormal karyotype was identified involving the long arm of chromosome 11. (lu.se)
  • Chromosome fusions and repetitive sequence accumulations might have occurred in C. semilaevis . (geneticsmr.com)
  • Contain a diploid set of chromosomes and make up the majority of human cells. (proprofs.com)
  • In 1993, Lee et al cloned a human complementary DNA, rBAT (renal basic amino acid transporter) in chromosome 2, encoding a transport protein for cystine and dibasic amino acids. (medscape.com)
  • Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. (innov-research.com)
  • Using comparative fluorescence in situ hybridization, strand-sequencing, single-molecule, real-time sequencing and Bionano optical mapping analyses, we investigated the organization of the 15q25 region in human and nonhuman primates. (pacb.com)
  • In the proximal region of the human X chromosome short arm, the position of evolutionary breakpoints with respect to key loci has been established as DMD-EB-XK-PFC-EB-GATA1-C1CN5-EB-DXS1272E-ALAS2-E B-DXF34-centromere. (mpi.nl)
  • Prader-Willi and Angelman syndrome (PWS and AS) patients typically have an ~5 Mb deletion of human chromosome 15q11-q13, of opposite parental origin. (biomedcentral.com)
  • BACKGROUND: The subtelomeric regions of human chromosomes exhibit an extraordinary plasticity. (anthropogeny.org)
  • Furthermore, we partially sequenced the underrepresented subtelomeres of human chromosomes showing a common evolutionary origin. (anthropogeny.org)
  • Chromosome 1 is the largest human chromosome, spanning about 249 million DNA building blocks (base pairs) and representing approximately 8 percent of the total DNA in cells. (medlineplus.gov)
  • Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)
  • We report a 7-year-old girl with spina bifida carrying a complex chromosome abnormality resulting in duplication 16q 12.1 -q22.1. (lu.se)
  • Factors involved in prediction of prognosis include the extent of the deletion, the occurrence of complex chromosome anomalies, and the severity of seizures. (medscape.com)
  • Constitutional chromosome studies involving conventional GTG band cytogenetic analysis, however, often show unbalanced karyotypes which cannot be fully defined because the extra material is too small to have a recognisable banding pattern. (bmj.com)
  • Ordering of 80 contigs along the deletion bins of chromosome arm 5DS revealed small-scale breaks in syntenic blocks. (biomedcentral.com)
  • A mouse model of PWS and AS has a transgenic insertion-deletion (TgPWS/TgAS) of chromosome 7B/C subsequent to paternal or maternal inheritance, respectively. (biomedcentral.com)
  • By isolating a Chrna7 -Tg fusion transcript from TgAS mice, we mapped the telomeric deletion breakpoint in Chrna7 intron 4. (biomedcentral.com)
  • Wolf-Hirschhorn syndrome results from the deletion of the distal short arm of chromosome 4. (medscape.com)
  • A large deletion several megabases (Mb) in length, easily detected using conventional chromosome analysis, is usually associated with severe phenotypic expression, including multiple malformations. (medscape.com)
  • They used molecular techniques to analyse the sex chromosomes of dragons, showing that the sex reversed females were genetically male. (edu.au)
  • The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
  • In females, there was an additional 18S rDNA signal mapping to the telomeric region of the W chromosome long arm. (geneticsmr.com)
  • Clonal populations of bacteria, each population maintaining a single artificial chromosome, are stored in various laboratories around the world. (wikipedia.org)
  • The artificial chromosomes (BAC) can be grown, extracted, and labeled, in any lab containing a library. (wikipedia.org)
  • With respect to the 5S rDNA, 12 signals were mapped to the centromeric regions of six pairs of autosomes. (geneticsmr.com)
  • In plants, polytene chromosomes have been observed in only a few species, and seemed to be restricted to ovary and immature seed tissues, e.g., in Phaseolus coccineus and P. vulgaris (Nagl, 1981), until relatively recently, when they were observed in the cells of the anther tapetum of Vigna unguiculata (Guerra and Carvalheira, 1994) and of some Phaseolus species (Carvalheira and Guerra, 1994). (scielo.br)
  • Bahri-Darwich I., Vaiman D., Olsaker I., Oustry A., Cribiu E.P., Assignment of bovine synteny groups U27 and U8 to R-banded chromosome 12 and 27, respectively, Hereditas 120 (1994) 261-265. (gse-journal.org)
  • The W chromosome is morphologically larger and has been considered evolutionarily younger than any other chromosome in the set. (geneticsmr.com)
  • Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)
  • The authors recently mapped a susceptibility locus for autism to chromosome region 2q24-q33 (MIM number 606053). (neurotransmitter.net)
  • Isolation and characterization of bovine chromosome region specific sequence-tagged microsatellite sites (STMS). (fbn-dummerstorf.de)
  • High-resolution comparative mapping of the proximal region of the mouse X chromosome. (mpi.nl)
  • The murine homologues of the loci for McLeod syndrome (XK), Dent's disease (CICN5), and synaptophysin (SYP) have been mapped to the proximal region of the mouse X chromosome and positioned with respect to other conserved loci in this region using a total of 948 progeny from two separate Mus musculus x Mus spretus backcrosses. (mpi.nl)
  • After checking all the necessary conditions, hybridization steps can be started by first adding a target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s). (wikipedia.org)
  • Two copies of chromosome 1, one copy inherited from each parent, form one of the pairs. (medlineplus.gov)
  • FBN3 (D6S24): a bovine microsatellite derived from cosmid cIOBT475 at chromosome 6q12-14. (fbn-dummerstorf.de)
  • Replication studies in the mother and daughter showed a skewed X inactivation pattern in lymphocytes, with the normal X chromosome preferentially inactivated. (bmj.com)
  • RUNX1 itself is the target of several chromosome rearrangements seen in leukemia. (nih.gov)
  • Isolation and application of chromosome 6 specific microsatellite markers for detection of QTL for milk-production traits. (fbn-dummerstorf.de)
  • Construction of comprehensive, informative second generation linkage maps for three Conifer genomes. (le.ac.uk)
  • However, the utility of such maps is limited and there is a recognised need to produce second generation (STS based) linkage maps represented by living pedigrees. (le.ac.uk)
  • Three Peromyscus linkage groups were assigned to chromosomes, based on linkage homology with Mus. (tamu.edu)
  • Felix Mitelman's initiative of mapping all chromosome aberrations in cancer has helped to improve diagnosis, treatment and prognosis for different types of cancer. (lu.se)
  • The establishment of synteny maps will provide a powerful tool to investigate the quantitative conservation of map location of trait loci across species and environments. (le.ac.uk)
  • Difficulties were encountered in preparing the working library probe from the chromosome 21 specific library and a major part of the work involved solving these problems. (gla.ac.uk)
  • almost all tumours have chromosome changes and some are typical of different types of cancer. (lu.se)
  • Polytene chromosomes are structures found in highly specialized tissues in some animal and plant species, which are amplified through successive cycles of endoreduplication, finally producing several copies of each chromosome. (scielo.br)
  • In other cases, individuals have a shorter or longer duplicated segment within the q21.1 region of chromosome 1. (medlineplus.gov)
  • The core phenotype maps within the terminal 1.9 Mb region of chromosome 4p. (medscape.com)