AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
CpG IslandsDNA MethylationMethylationEpigenesis, GeneticDNA (Cytosine-5-)-MethyltransferasePromoter Regions, GeneticSulfitesAzacitidineCytosineGene SilencingMethyltransferasesDNA Modification MethylasesHistones5-MethylcytosineGenomic ImprintingEpigenomicsHistone-Lysine N-MethyltransferaseDNA-Cytosine MethylasesLong Interspersed Nucleotide ElementsProtein MethyltransferasesS-AdenosylmethionineBase SequenceLysineDinucleoside PhosphatesProtein-Arginine N-MethyltransferasesMolecular Sequence DataDNA, NeoplasmDNAPolymerase Chain ReactionGene Expression Regulation, NeoplasticGenes, p16RNA, Long NoncodingDeoxyribonuclease HpaIIS-AdenosylhomocysteineSequence Analysis, DNAChromatinCell Line, TumorDeath-Associated Protein KinasesTranscription, GeneticTumor Suppressor ProteinsSite-Specific DNA-Methyltransferase (Adenine-Specific)Genes, Tumor SuppressorGenome, HumanCyclin-Dependent Kinase Inhibitor p16Gene Expression RegulationAcetylationMutationRNA, UntranslatedHeterochromatinAlu ElementsNuclear ProteinstRNA MethyltransferasesReverse Transcriptase Polymerase Chain ReactionCell LineOligonucleotide Array Sequence AnalysisDNA-Binding ProteinsGene Expression ProfilingRNA, MessengerRepressor ProteinsCyclin-Dependent Kinase Inhibitor p15Chromatin ImmunoprecipitationGenetic LociDNA PrimersTranscription FactorssnRNP Core ProteinsHistone DemethylasesAllelesInsulin-Like Growth Factor IIDNA, PlantTumor Markers, BiologicalGlutathione S-Transferase piColorectal NeoplasmsO(6)-Methylguanine-DNA MethyltransferaseDNA Repair EnzymesGenomeArabidopsisProtein O-MethyltransferaseHydroxamic AcidsNeoplasm ProteinsEuchromatinGenes, NeoplasmJumonji Domain-Containing Histone DemethylasesMethionineTubercidinPhenotypeModels, GeneticBlotting, SouthernCore Binding Factor Alpha 3 SubunitChromatin Assembly and DisassemblyArginineCytidinePolycomb-Group ProteinsProtein Processing, Post-TranslationalFolic AcidEmbryonic Stem CellsAmino Acid SequenceRepetitive Sequences, Nucleic AcidProtein BindingBinding SitesTumor Cells, Cultured
GenesPatternsGlobal DNA methylationLysine methylationGenomeLevel of DNA methylationStudies of methylationCpGsAberrantMethodsTranscriptionalGeneticGene methylationCytosine methylationLociAssayPeripheral bloodAltersAnalysisPrefrontal cortexEpigeneticsLevelsCorrelationProcessesDetermined by chromatinProteinsTranscription factorPromotersRegulate gene expressionBiologicalPathwaysDifferentialMarkersSomaticEukaryotesChromatin structureOncologyResiduesDistinctCohortsMechanismsModificationsOccurPromoter regionDifferencesProfilesClinicalTumourParkinson'sMethyltransferases
Genes24
  • Methylation levels at three age-related CpGs--located in the genes ITGA2B, ASPA and PDE4C--were subsequently analyzed by bisulfite pyrosequencing of 151 blood samples. (nih.gov)
  • Probes on the 27k array target regions of the human genome to measure methylation levels at 27,578 CpG dinucleotides in 14,495 genes. (wikipedia.org)
  • A large scale measurement of DNA methylation patterns from a wide selection of genes may enable us to understand better the relationships between epigenetic changes and the genesis of different diseases and a better understanding of the role that epigenetics plays in tissue specific differentiation. (wikipedia.org)
  • The Illumina 27k methylation chip contains 27,578 individual CpG sites, spread across 14,495 genes. (wikipedia.org)
  • These genes include RefSeq genes from the NCBI CCDS Database, cancer genes that show differential methylation patterns during their course of progression and microRNA promoters. (wikipedia.org)
  • Sitting time may affect health by altering the methylation of certain genes. (nature.com)
  • We found that the most significant differentially methylated sites had a remarkable enrichment in type I interferon (IFN) pathway genes. (frontiersin.org)
  • In conclusion, our study demonstrated that hypomethylation of IFN-related genes is a common feature of GD/RA/SLE/SSc patients in CD4+ T cells, and the DNA methylation profile of IFN-related genes could be promising biomarkers for the diagnosis of GD, RA, SLE, and SSc. (frontiersin.org)
  • Methylation profile of the promoter CpG islands of 14 "drug-resistance" genes in hepatocellular carcinoma. (wjgnet.com)
  • To establish the DNA methylation patterns of the promoter CpG islands of 14 "drug-resistance" genes in hepatocellular carcinoma (HCC). (wjgnet.com)
  • The methylation specific polymerase chain reaction in conjunction with sequencing verification was used to establish the methylation patterns of the 14 genes in the liver tissues of four healthy liver donors, as well as tumor and the paired non-cancerous tissues of 30 HCC patients. (wjgnet.com)
  • Aberrant methylation of CpG islands in the promoter region of genes is a common epigenetic phenomenon found in early cancers. (spandidos-publications.com)
  • Among 29 genes obtained by cross-comparison of the two gene-sets, we subsequently selected, through stepwise subtraction processes, two novel genes, GABRA1 and LAMA2, as methylation targets in colorectal cancer. (spandidos-publications.com)
  • 0.05) associated with differential DNA methylation of 31 CpGs annotated to 29 genes. (bmj.com)
  • In animals and plants, methylation - the addition of methyl groups to specific DNA sites - is known to turn off genes. (vetscite.org)
  • When E. coli O104:H4 became infected with the phage, not only were thousands of methylation sites added, but more than a third of the bacteria's genes had altered patterns of expression. (vetscite.org)
  • RESULTS: DNA methylation changes in neuroblastoma affect not only promoters but also intragenic and intergenic regions at cytosine-phosphate-guanine (CpG) and non-CpG sites, and target functional chromatin domains of development and cancer-related genes such as CCND1. (ca.gov)
  • Non-CpG methylation observed essentially in clinically favorable cases was associated with the differentiation status of neuroblastoma and expression of key genes such as ALK. (ca.gov)
  • In summary, DNA methylation changes in KMT2Ar ALL target enhancers, genes involved in leukemogenesis and normal hematopoiesis, as well as transcription factor networks. (scilifelab.se)
  • Among the 30 genes we tested, 14 genes showed a negative correlation between methylation level and expression level. (bvsalud.org)
  • Next, Gabel and his team plan to assess whether other genes associated with autism affect the methylation at CA sites. (spectrumnews.org)
  • Among the 54 genes mapping to these enriched pathways, DNA methylation of CpGs mapping to PRKCA and TSPO correlated with their own mRNA expression (HumanHT-12 Expression BeadChip). (lu.se)
  • Typically, methylation turns genes "off" and demethylation turns genes "on. (cdc.gov)
  • DNA methylation is a chemical process through which methyl groups attach to the DNA molecule, affecting the function of genes. (lu.se)
Patterns10
  • Distinct promoter methylation patterns arise upon clonal selection in different families of cell lines. (bournemouth.ac.uk)
  • Thus, cell-type-specific regulatory patterns most likely depend on methylation in the CpG context. (biomedcentral.com)
  • These results indicate differences in DNA methylation patterns between breeds and it remains to be established whether variation in DNA methylation patterns impacts on phenotypic traits. (eurekamag.com)
  • Noma, K., Allis, C.D. & Grewal, S.I. Transitions in distinct histone H3 methylation patterns at the heterochromatin domain boundaries. (nature.com)
  • We conducted an integrative analysis of the multiple-autoimmune disease methylation dataset including GD, RA, SLE, and SSc samples, to identify the common methylation patterns of autoimmune diseases. (frontiersin.org)
  • Waldor's group went even further than analyzing a single gene: they used SMRT to map the methylation patterns of the entire E.coli O104:H4 genome. (vetscite.org)
  • Phase variation of a Type IIG restriction-modification enzyme alters site-specific methylation patterns and gene expression in Campylobacter jejuni strain NCTC11168. (pacb.com)
  • Methylation patterns from cancer tumours. (lu.se)
  • By studying the twins' DNA over a long period, it was seen that from identical methylation patterns at the age of three, their patterns diverged as they grew older. (lu.se)
  • We will also examine differences in methylation patterns among responders with (a) comorbid WTC-PTSD and respiratory illness, (b) only respiratory illness, (c) only WTC-PTSD, and (d) neither respiratory illness nor WTC-PTSD. (cdc.gov)
Global DNA methylation4
Lysine methylation2
  • Lachner, M. & Jenuwein, T. The many faces of histone lysine methylation. (nature.com)
  • Lysine methylation marks manifest their biological effect via so-called 'readers' (or reading domains) which recognize and bind the methylation mark and directly alter the chromatin structure or act as a scaffold for other proteins, which induce biological responses. (europa.eu)
Genome21
  • Human aging is associated with DNA methylation changes at specific sites in the genome. (nih.gov)
  • Viral DNA isolated from a single wart was partially methylated at only one out of the four HpaII sites, d(C-C-G-G). Recognition sites for Bg/I, Bg/II, PstI and PvuII restriction endonucleases were located on the cloned genome. (pasteur.fr)
  • The Illumina Methylation Assay using the Infinium I platform uses 'BeadChip' technology[clarification needed] to generate a comprehensive genome-wide profiling of human DNA methylation. (wikipedia.org)
  • Similar to bisulfite sequencing and pyrosequencing, this method quantifies methylation levels at various loci within the genome. (wikipedia.org)
  • In 2016, the Infinium MethylationEPIC BeadChip was released, which interrogates over 850,000 methylation sites across the human genome. (wikipedia.org)
  • Here we determined genome-wide changes in DNA methylation in the nervous system in neuropathic pain. (jneurosci.org)
  • However, genome-wide changes in DNA methylation in the nervous system and their roles in neuropathic pain development remain unclear. (jneurosci.org)
  • Here we used digital restriction enzyme analysis of methylation to quantitatively determine genome-wide DNA methylation changes caused by nerve injury. (jneurosci.org)
  • We interpret different turnover rates in light of genome-wide localization data and transcription-dependent nucleosome rearrangements proximal to the transcription start site. (figshare.com)
  • Therefore conducting genome-scale methylation studies will enhance our understanding of the epigenetic etiology behind carcinogenesis by providing reliable biomarkers for early detection of cancer. (spandidos-publications.com)
  • Therefore, we assessed associations between occupational exposure to pesticides and genome-wide DNA methylation sites. (bmj.com)
  • In addition, we performed genome-wide stratified and interaction analyses by gender, smoking and airway obstruction status, and assessed associations between gene expression and methylation for genome-wide significant CpGs (n=2802). (bmj.com)
  • Conclusions We show for the first time that occupational exposure to pesticides is genome-wide associated with differential DNA methylation. (bmj.com)
  • A new DNA sequencing technique has enabled researchers to map for the first time the influential chemical modifications known as methylation marks throughout the genome of a pathogenic bacterium. (vetscite.org)
  • The concept of MRS is similar to that of PRS, but instead of genetic variants, it considers the methylation status at multiple genome locations. (cdc.gov)
  • MATERIALS & METHODS: Microarray DNA methylation data were analyzed and associated with functional/regulatory genome annotation data, transcriptional profiles and clinicobiological parameters. (ca.gov)
  • Here, we characterized the mtDNA methylation landscape in the prefrontal cortex of neurological healthy individuals (n=26) and patients with Parkinson's disease (n=27), using a combination of whole-genome bisulphite sequencing (WGBS) and bisulphite-independent methods. (sens.org)
  • The mice's neurons have nearly half the usual amount of CA methylation, the researchers found, especially at enhancers, portions of the genome that can regulate gene expression. (spectrumnews.org)
  • Although SFAs have been shown to affect the epigenome in whole blood, pancreatic islets, and adipose tissue in humans, and genome-wide DNA methylation studies have linked epigenetic changes to NAFLD and NASH, studies focusing on the association of SFAs and DNA methylation in human liver are missing. (lu.se)
  • Charlotte Ling and her research group have studied DNA methylations in the genome of different patient groups with type 2 diabetes. (lu.se)
  • The authors measured DNA methylations in the blood at 800.000 sites in the genome of all participants. (lu.se)
Level of DNA methylation2
  • Conclusions: Our results suggest that one of the mechanisms how SFA could contribute to metabolic dysregulation in NAFLD is at the level of DNA methylation. (lu.se)
  • The level of DNA methylation decreases with age. (cdc.gov)
Studies of methylation2
CpGs5
  • Associations between pesticide exposure and 420 938 methylation sites (CpGs) were assessed using robust linear regression adjusted for appropriate confounders. (bmj.com)
  • filematrix with the location of the CpGs / SNPs / gene trascription start sites. (bioconductor.org)
  • The CpGs single-sites at positions -166 and -146 were completely methylated, while the site -102 was totally unmethylated, independently of the presence of AP. (uandes.cl)
  • Of these 3169 sites, 797 overlapped with previously published NASH-associated CpGs (NASH-SFA), while 2372 CpGs were exclusively associated with SFA (Only-SFA). (lu.se)
  • The proportion of CpGs demonstrating a correlation of DNA methylation with plasma glucose was higher in NASH-SFA and only-SFA groups, while the proportion of significant correlations with plasma insulin was higher in only-NASH and NASH-SFA groups as compared to all CpGs on the Illumina 450 K array (Illumina, San Diego, CA, USA). (lu.se)
Aberrant2
  • Aberrant methylation at target CpG sites in GABRA1 and LAMA2 was observed with high frequency in tumor tissues (92.5% and 80.6%, respectively), while less frequently in matched tumor-adjacent normal tissues (33.6% for GABRA1 and 13.4% for LAMA2). (spandidos-publications.com)
  • Aberrant methylation is a widespread phenomenon in cancer and may be among the earliest changes to occur during oncogenesis (Stirzaker, et al. (thermofisher.com)
Methods1
  • Methods: With a sample size of 197 firefighters, we quantified the serum concentrations of nine PFASs, blood leukocyte DNA methylation and epigenetic age indicators via the EPIC array. (cdc.gov)
Transcriptional3
  • We found that the methylation profiles of 16.6% of cytosines and the expression profiles of neighboring transcriptional start sites (TSSs) were significantly negatively correlated. (biomedcentral.com)
  • Kouzarides, T. Histone methylation in transcriptional control. (nature.com)
  • Oncology is one of the disciplines where DNA methylation technology is widely employed to develop therapeutic options for rectifying the cancer epigenome's transcriptional aberrations. (medgadget.com)
Genetic4
  • DNA methylation plays a significant role in the epigenetic regulation of chromatin structure, which in the last decade has been recognized to be important in the regulation of gene expression, development and genetic imprinting in vertebrates. (wikipedia.org)
  • This concept has spread to epigenetics, particularly DNA methylation, to create methylation risk scores (MRS). Epigenetics involves the alteration of gene expression without changing the genetic code through processes such as DNA methylation and histone modification. (cdc.gov)
  • The epigenetic regulation through methylation at F7 promoter as well as the inter-relationship of genetic and epigenetic modifications at F7 promoter site were both previously unexplored. (bmj.com)
  • BDNF promoter methylation and genetic variation in late-life depression. (zymoresearch.com)
Gene methylation2
  • DNA methylation is an important regulator of gene transcription, and global and specific gene methylation marks may vary with cigarette smoke exposure. (nih.gov)
  • Aim: To determine TLR2 gene methylation and inflammatory profiles of PBMCs in AP. (uandes.cl)
Cytosine methylation2
Loci3
  • Significant changes in DNA methylation in KMT2Ar ALL were identified in 2,545 CpG loci, influenced by age and the translocation partners AFF1 and MLLT1. (scilifelab.se)
  • Eventually, we found that the methylation level of 1233 gene loci was changed in the exposure group. (bvsalud.org)
  • We generated a set of sgRNAs targeting multiple sites within the human EMX1 and PVALB loci with different tracrRNA 3′ truncations ( Fig. 1a ). (cdc.gov)
Assay1
  • This assay is used for methylation probes on the Illumina Infinium HumanMethylation27 BeadChip (henceforth, 27k [methylation] array). (wikipedia.org)
Peripheral blood3
  • Changes in DNA methylation in peripheral blood could be associated with malignant diseases. (uni-koeln.de)
  • In this study, we measured the DNA damage levels and the alternation of DNA methylation in peripheral blood mononuclear cells (PBMCs) collected from a population exposed to e-waste. (bvsalud.org)
  • Specifically, we will determine and compare the methylation pattern of the peripheral blood DNA of patients with WTC-PTSD and patients who never developed PTSD. (cdc.gov)
Alters2
Analysis17
  • We perform a comprehensive analysis of methylation profiles to narrow down 102 age-related CpG sites in blood. (nih.gov)
  • Analysis of methylation data The scanned microarray images of methylation data are further analyzed by the system, which normalizes the raw data to reduce the effects of experimental variation, background and average normalization, and performs standard statistical tests on the results. (wikipedia.org)
  • However, the analysis of a single CpG site or a few CpG sites as surrogate indicators of DNA methylation status of the surrounding region is the most prevalent strategy in epigenetic studies at different scales, due to the assumption of the relatively homogeneous distribution of DNA methylation within genomic regions. (biomedcentral.com)
  • The putative IGF2 differentially methylated regions (DMR) 0, 1 and 2, a quantitative trait nucleotide (QTN) region in the intron 3 and a CpG island in the intron 4 of the IGF2 gene as well as three regions around porcine CTCF binding sites within the H19 differentially methylated domain (DMD) were selected for the DNA methylation analysis. (eurekamag.com)
  • The methylation analysis revealed significantly more methylated CpG sites in the intron 4 of sperm from the LW breed than in that from SL. (eurekamag.com)
  • The m.545 methylation was validated using a combination of methylation-sensitive DNA digestion and quantitative PCR analysis. (sens.org)
  • In addition, we propose a robust framework for methylation analysis of mtDNA from WGBS data, which is less prone to false-positive findings due to misalignment of nuclear mitochondrial pseudogene sequences. (sens.org)
  • However, RaMWAS can also be useful for the analysis of methylation measurements from other platforms (e.g. (bioconductor.org)
  • This large-scale analysis suggests that DNA methylation may be a biomarker of COPD and may highlight new pathways of COPD pathogenesis. (nih.gov)
  • In April 2019, New England Biolabs has released a new product called 'NEBNext Enzymatic Methyl-seq (EM-seqTM)' as a methylation analysis alternative to bisulfite sequencing. (medgadget.com)
  • To investigate the underlying molecular principles of metabolic and morphogenic zonation of the human liver lobule, we generated an integrated epigenetic map across three zones (pericentral, intermediate and periportal) by methylation and transcriptomic analysis of hepatocytes captured by laser micro-dissection. (lisym.org)
  • Fastest bisulfite conversion kit for complete bisulfite conversion of DNA for methylation analysis. (zymoresearch.com)
  • The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis. (zymoresearch.com)
  • Our comprehensive range of products for DNA methylation analysis enables optimal results from a wide range of sample types, allowing you to detect and quantify even small changes in methylation level. (qiagen.com)
  • and research using the Sequenom MassArray system for SNP genotyping, DNA methylation analysis, and somatic mutation profiling. (genomeweb.com)
  • 1992), the bisulfite conversion technique has become a preferred tool for methylation analysis. (thermofisher.com)
  • They're based on the analysis of DNA methylation, a biological process by which CH3 methyl groups are added to the DNA molecule and that determines gene expression. (medscape.com)
Prefrontal cortex1
Epigenetics1
  • A new study co-led by researchers from the Epigenetics Group at the International Agency for Research on Cancer (IARC) provides a comprehensive catalogue of DNA methylation markers of gestational age at birth, a characteristic that is associated with the onset of diseases in later life. (who.int)
Levels12
  • In addition, the level of methylation of the HpaII sites (CCGG) within CGIs demonstrates a correlation with average CGI methylation levels [ 10 ]. (biomedcentral.com)
  • Moreover, Chr9: 4985407 site and Chr9: 4985238-4985455 region methylation levels mediated the association of sitting time with abdominal obesity, and the indirect effects account for 6.78% and 4.24%, respectively. (nature.com)
  • We hypothesized that maternal gestational glucose levels would positively correlate with DNA methylation of the PPARGC1A promoter in placental tissue. (portlandpress.com)
  • Methylation levels in primary tumors were not significantly correlated with clinico-pathological features including age, sex, survival and TNM stage. (spandidos-publications.com)
  • Blood DNA methylation levels were measured using Illumina 450K arrays. (bmj.com)
  • D 3 M: Detection of Differential Distributions of Methylation levels. (github.com)
  • The highest methylation levels (5.49 ± 0.97%) were found on CpG position m.545, located in the heavy-strand promoter 1 region. (sens.org)
  • Interestingly, m.545 methylation correlated with the levels of mtDNA transcripts, suggesting a putative role in regulating mtDNA gene expression. (sens.org)
  • Bisulfite conversion will work regardless of context, so the kits are compatible with genomic DNA derived from plants and other species with high non-CpG methylation levels. (zymoresearch.com)
  • For most of those sites, the methylation levels changed with age during early childhood and stabilized after school age (7 years). (who.int)
  • The researchers found that the four subgroups had different levels of DNA methylation at 4.465 sites. (lu.se)
  • sgRNAs with +67 or +85 nucleotide (nt) tracrRNA tails mediated DNA cleavage at all target sites tested, with up to fivefold higher levels of indels than the corresponding crRNA-tracrRNA duplexes ( Fig. 1b and Supplementary Fig. 1a ). (cdc.gov)
Correlation1
  • The correlation between a polymorphism and its effect on DNA methylation and, consequently, on gene product regulation is a novel and very intriguing concept. (bmj.com)
Processes4
  • Our next step will be to find small molecules that target the cyclical methylation processes to elucidate their precise role. (sciencedaily.com)
  • His fields of research comprise RNA-mediated gene silencing processes with a focus on epigenetic phenomena, including studies on RNA-directed DNA methylation, the characterization of virus silencing suppressor proteins, the development of plant bioreactor platforms and viroid research. (degruyter.com)
  • Iodomethane is off-gas into air and be transported off-site by meteorologi- approved in the United States for pre-plant application into cal processes. (cdc.gov)
  • Occupational exposure to an intermediate in the manufacture of some pharmaceu- iodomethane may occur 8 hours per day, 5 days per week, ticals, in methylation processes, and in the field of micro- during the application season. (cdc.gov)
Determined by chromatin1
Proteins2
Transcription factor3
  • In this article, we extend the characterization to include CMV promoter methylation and its influence on NFκB and CREB1 transcription factor binding to the CMV promoter in two families of DHFR-amplified CHO cell lines. (bournemouth.ac.uk)
  • To overcome Sanger-sequencing limitations due to high CG bias and multiple transgenes copies, pyrosequencing was used to determine the frequency of methylated cytosines in regions proximal to and containing the NFκB and CREB1 transcription-factor consensus binding sites. (bournemouth.ac.uk)
  • In KMT2Ar ALL, DNA methylation loss was enriched at enhancers and for certain transcription factor binding sites such as BCL11A, EBF, and MEF2A. (scilifelab.se)
Promoters1
Regulate gene expression1
Biological3
  • Variation of age predictions correlates moderately with clinical and lifestyle parameters supporting the notion that age-associated methylation changes are associated more with biological age than with chronological age. (nih.gov)
  • DNA methylation fingerprint of neuroblastoma reveals new biological and clinical insights. (ca.gov)
  • Methionine, in turn, is required for the synthesis of S-adenosylmethionine (SAM), a methyl group donor used in many biological methylation reactions, including the methylation of sites in DNA and RNA. (medscape.com)
Pathways1
  • Many of the top associations between COPD and DNA methylation occur in biologically plausible pathways. (nih.gov)
Differential2
  • Further research should reveal whether this differential methylation plays a role in the airway disease pathogenesis induced by pesticides. (bmj.com)
  • In addition, the blood-based S100P methylation pattern was similar among BC patients with differential clinical characteristics regardless of stage, receptor status and menopause status. (uni-koeln.de)
Markers3
  • This study reveals significant association between blood-based decreased S100P methylation and BC, and provides another proof for the application of altered DNA methylation signatures from blood cells as potential markers for the detection of BC, especially for the early stage. (uni-koeln.de)
  • Circulating tumour DNA methylation markers for diagnosis and prognosis of hepatocellular carcinoma. (zymoresearch.com)
  • The researchers found a clear overlap of methylation markers in cord blood, fetal brain tissue, and fetal lung tissue in relation to gestational age at birth, highlighting the consistency of these findings across tissues relevant for the health impacts of gestational age at birth. (who.int)
Somatic1
Eukaryotes2
Chromatin structure2
Oncology1
Residues1
Distinct1
  • In short, whether or not the distinct methylation status of a single CpG affects specific transcription-related functions remains an open question. (biomedcentral.com)
Cohorts3
  • We conducted array-based methylation screens, using a test-replication approach, in two family-based cohorts (n = 1,085 and 369 subjects). (nih.gov)
  • We observed 349 CpG sites significantly associated with the presence and severity of COPD in both cohorts. (nih.gov)
  • On the basis of almost 7000 cord blood samples from 20 cohorts worldwide, about 9000 methylation sites were found to be associated with gestational age at birth. (who.int)
Mechanisms3
  • One of these mechanisms is DNA methylation, where a small chemical residue called a methyl group is added to strategic bases on the DNA. (sciencedaily.com)
  • DNA methylation is one of the major epigenetic mechanisms, and it plays an important role in determining gene function ( Hedrich and Tsokos, 2011 ). (frontiersin.org)
  • A potential mechanism might be through epigenetic mechanisms, like DNA methylation. (bmj.com)
Modifications2
Occur1
  • They found out that methylation marks occur rapidly in breast cancer cells in response to hormones such as estrogen or drug compounds. (sciencedaily.com)
Promoter region2
  • In the other cell line family, lower methylation frequency at the NFκB consensus binding site was accompanied by more NFκB recruitment to the promoter region. (bournemouth.ac.uk)
  • DNA was bisulfite-treated, and specific sites at the promoter region of the TLR2 gene were amplified by qPCR using validated primers. (uandes.cl)
Differences2
  • Significant differences in DNA methylation content were found between the two breeds in the two DMD regions proximal to the H19 gene. (eurekamag.com)
  • Tumors with diverse clinical risk showed differences affecting CpG and, remarkably, non-CpG sites. (ca.gov)
Profiles2
Clinical3
  • For clinical validation pyrosequencing was used to assess methylation in 134 matched tissue samples from CRC patients. (spandidos-publications.com)
  • From Polygenic Risk Scores to Methylation Risk Scores: What are the Clinical Applications? (cdc.gov)
  • For example, in the recent study that used MRS in addition to electronic health records (EHR) to predict clinical phenotypes, the methylation was measured after clinical diagnosis. (cdc.gov)
Tumour1
  • Furthermore, methylation-dependent silencing of SOX11 has been reported for B cell lymphomas and indicates that epigenetic drugs may be used to re-express this tumour suppressor, but information on SOX11 promoter methylation in EOC is still lacking. (lu.se)
Parkinson's1
  • We detected no association between mtDNA methylation profile and Parkinson's disease. (sens.org)
Methyltransferases1