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  • induces
  • Furthermore, siRNA transfection induces up-regulated expression of both GW182, a key P-body component, and Ago2, indicating that P-body localization and interaction with GW182 and Ago2 are important in siRNA-mediated RNAi. (pubmedcentralcanada.ca)
  • Drosophila
  • Here we investigated if RNAi indeed provides protection against virus infection in Drosophila embryos and adults. (pubmedcentralcanada.ca)
  • However, abundant accumulation of R1ΔB2 ( Fig. 1 , lane 9), but not of FR1fs ( Fig. 1 , lane 7), occurred in the mutant Drosophila embryos that carried a homozygous null mutation in ago-2 ( ago-2 414 ), which is essential for RNAi in Drosophila ( 1 , 12 , 13 ). (pubmedcentralcanada.ca)
  • These data indicated that viral RNA replication in Drosophila embryos triggers an RNAi-mediated virus clearance in an Ago-2 -dependent manner and effective RNAi suppression by B2 is necessary to achieve normal accumulation of FHV RNAs. (pubmedcentralcanada.ca)
  • A year later, the lin-4 and let-7 RNAs were found to be part of a large class of small RNAs present in C. elegans, Drosophila and human cells. (wikipedia.org)
  • RNAi in organisms such as C. elegans and Drosophila melanogaster provides a quick and inexpensive means of investigating gene function. (wikipedia.org)
  • backbone
  • However, without modifications to the RNA backbone or inclusion of inverted bases at either end, siRNA instability in the plasma makes it extremely difficult to apply this technique in vivo. (wikipedia.org)
  • cytokines
  • In addition, delivery of siRNAs is subject to endosomal trafficking, potentially exposing them to TLR3 and TLR7, and can lead to activation of interferons and proinflammatory cytokines. (wikipedia.org)
  • In addition, in contrast to unmodified or modified and non-encapsulated siRNA, administration of SNALP-delivered siRNA resulted in no detectable levels of interferons, such as IFN-a, or inflammatory cytokines associated with immunostimulation. (wikipedia.org)