Single molecule detection. Medical search. Frequent questions

https://github.com/fritzsedlazeck/Sniffles ) that provide unprecedented sensitivity and precision for variant detection, even in repeat-rich regions and for complex nested events that can have substantial effects on human health. (nature.com)
The system is an efficient disposable lens element that enables single molecule sensitivity at low picomolar concentration. (fresnel.fr)
10] P. Ghenuche, H. Rigneault, J. Wenger, Hollow-core photonic crystal ﬁber probe for remote ﬂuorescence sensing with single molecule sensitivity, Opt. (fresnel.fr)
Fluorescence is a convenient means of observing one molecule at a time, mostly due to the sensitivity of commercial optical detectors, capable of counting single photons. (wikipedia.org)
However, spectroscopically, the observation of one molecule requires that the molecule is in an isolated environment and that it emits photons upon excitation, which owing to the technology to detect single photons by use of photomultiplier tubes (PMT) or avalanche photodiodes (APD), enables one to record photon emission events with great sensitivity and time resolution. (wikipedia.org)
Now, scientists at the Wyss Institute for Biologically Inspired Engineering at Harvard University and Brigham and Women's Hospital (BWH) have created a new type of immuno-assay that is capable of detecting small molecules with 50-fold greater sensitivity than conventional detection methods, and can be easily integrated into existing diagnostic platforms. (harvard.edu)
The enhanced analytical sensitivity of our assay enables measurements of small molecules at extremely low concentrations, and opens a window into biological phenomena that were previously unreachable," said senior author David Walt, Ph.D., a Core Faculty member of the Wyss Institute who is also the Hansjörg Wyss Professor of Biologically Inspired Engineering at Harvard Medical School (HMS) and Professor of Pathology at BWH, as well as an HHMI Professor. (harvard.edu)
Proximity ligation and extension technologies can offer the required sensitivity and specificity for such analyses, allowing detection of even single protein molecules in situ or in solution phase. (selectbiosciences.com)
Signal detection sensitivity may be increased through controllable noise generation. (nanowerk.com)
Nanopores are versatile single-molecule sensors offering a simple label-free readout with great sensitivity. (tudelft.nl)
Analysis at the single-cell level is essential for the understanding of cellular responses in heterogeneous cell populations, but it has been difficult to perform because of the strict requirements put on detection methods with regard to selectivity and sensitivity (i.e., owing to the cross-reactivity of probes and limited signal amplification). (nih.gov)
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. (sigmaaldrich.com)
We use nanowire-enhanced surfaces for the detection of fluorescent molecules with extremely high sensitivity, for research and applications. (lu.se)
bacteria culture is the gold standard in TB detection for its highest sensitivity, but requires weeks to obtain results. (cdc.gov)

7] J. Wenger, H. Rigneault, Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection, Int. J. Mol. (fresnel.fr)
We developed a new method of single molecule fluorescence detection that can track single-molecule fluorescence and FRET efficiency at the time resolution of 10 microsecond. (nii.ac.jp)

Single-molecule long-read sequencing has the potential to dramatically advance the field, although high error rates are a challenge with existing methods. (nature.com)
We characterize in real time the composition and catalytic state of the initial Escherichia coli transcription-coupled repair (TCR) machinery by using correlative single-molecule methods. (nature.com)
The issued claims cover methods for capturing, amplifying, and imaging single copies of target nucleic acid molecules. (bioratherapeutics.com)
This patent covers critical methods for counting target molecules, obviating the need for sequencing," said Matthew Cooper, General Manager, Diagnostics. (bioratherapeutics.com)
To overcome these hurdles, we developed methods for PTM detection using Quantum-Si's PlatinumTM single-molecule protein sequencing platform. (quantum-si.com)
We developed and used new single-molecule fluorescence spectroscopic and fluorescence lifetime imaging methods, combined with deep learning for image analysis. (biorxiv.org)
The interaction of biotin and Avidin or Streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. (genomicglossaries.com)
Both padlock probes and in situ PLA provide the ability to directly visualize single molecules by standard microscopy in fixed cells or tissue sections, and these methods may thus be valuable for both research and diagnostic purposes. (nih.gov)
Ultra-sensitive detection methods are required for diagnosing early-stage cancers," Diao said. (uc.edu)
CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)

Fluorescence Correlation Spectroscopy (FCS) in its modern embodiment measures and exploits temporal fluctuations in the fluorescence emission of small numbers of molecules in an open excitation volume (of ca 1 fl) defined by confocal optics. (dbkgroup.org)
Using force spectroscopy, single molecules (or pairs of interacting molecules), usually polymers, can be mechanically stretched, and their elastic response recorded in real time. (wikipedia.org)
In 1997, single-molecule detection was demonstrated with surface-enhanced Raman spectroscopy (SERS) by K. & H. Kneipp, Y. Wang, L.T. Perelman and others at MIT and independently by S. Nie and S. R. Emory at Indiana University. (wikipedia.org)
Single-molecule spectroscopy can be an effective tool to probe heterogeneity because molecular species can be observed one at a time, so individual oligomers can be detected without separation. (biorxiv.org)
In this initial study, we combine single-molecule Förster resonance energy transfer (FRET) spectroscopy, fluorescence lifetime imaging (FLIM), and image analysis using deep learning (codes are available at https://github.com/hoisunglab/FNet ) to interrogate several steps during the aggregation process of the 42-residue Aβ peptide (Aβ42), including dimerization, formation of stable oligomers, and fibril elongation. (biorxiv.org)
The experimental method is a three-dimensional imaging technique using a time-of-flight spectroscopy with multiparticle position sensitive detection. (lu.se)
R. Camacho-Dejay, " Polarization portraits of lightharvesting antennas: from single molecule spectroscopy to imaging " PhD-Thesis, Lund University, 2014 . (lu.se)

The fewer the number of fluorescent wells, the fewer labeled target molecules are bound to the beads, and thus the greater the concentration of the unlabeled target molecule present in the sample. (harvard.edu)
The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)
During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
The microparticles, including the captured target molecules bound to them, are pulled to the side of the reaction vessel using magnets and the supernatant is aspirated. (cdc.gov)
Simoa uses the same reagents as conventional ELISA, but antibody capture agents are attached to the surface of paramagnetic beads that are used to concentrate a dilute solution of target molecules. (lu.se)

The new assay for small molecules uses the existing Simoa detection system, shown here, which is able to identify single molecules of a target substance bound to magnetic microbeads. (harvard.edu)
The talk discusses a single cell assay that enables time lapse studies of single cell behaviors and single cell secretions under perturbations such as drugs, pH changes, transforming growth factors, etc. (selectbiosciences.com)
The science that grew from that collaboration led first to an early pancreatic cancer detection procedure that received Food and Drug Administration breakthrough designation, then continued to grow at UC's Vontz Center for Molecular Studies, where Diao has perfected a new assay for diagnosing cancer and cancer progression that is cheaper than current procedures, faster, less invasive and, most importantly, more accurate. (uc.edu)
Most recently, GeneXpert MTB/RIF (Cepheid) advanced nucleic acid-based detection by integrating PCR and a highly sensitive molecular-beacon assay into a single, automated system. (cdc.gov)
A menu of Simoa assay kits (single plex and multiplex) will be available to measure critical biomarkers. (lu.se)

The MIT team used non-resonance Raman excitation and surface enhancement with silver nanoclusters to detect single cresyl violet molecules, while the team at Indiana University used resonance Raman excitation and surface enhancement with silver nanoparticles to detect single rhodamine 6G molecules. (wikipedia.org)
Ulta-Bright Fluorescent Organic Nanoparticles based on Small-Molecule Ionic Isolation Lattices , Junsheng Chen, S. M. Ali Fateminia, Laura Kacenauskaite, Nicolai Bærentsen, Karen L. Martinez, Amar H. Flood, Bo W. Laursen. (lu.se)

A single-molecule experiment is an experiment that investigates the properties of individual molecules. (wikipedia.org)
Since many measurement techniques in biology, chemistry, and physics are not sensitive enough to observe single molecules, single-molecule fluorescence techniques (that have emerged since the 1990s for probing various processes on the level of individual molecules) caused a lot of excitement, since these supplied many new details on the measured processes that were not accessible in the past. (wikipedia.org)
Indeed, since the 1990s, many techniques for probing individual molecules have been developed. (wikipedia.org)
Abstract: This thesis covers the usage of the fiber arrays developed in our lab for sensitive detection of individual molecules. (tufts.edu)
identify different approaches to optical problems in biophysics and biomedicine from a broad perspective ranging from individual molecules to tissue. (lu.se)

We are particularly interested in understanding how the geometry of a molecule changes: one example is isomerization or proton transfer that is driven by vibrational excitation. (lu.se)
This allows to record the fluorescence intensity of a single molecule as a function of two arguments: excitation polarisation plane orientation angle φ ex and allowed emission polarisation plane orientation angle φ em . (lu.se)

Besides, atomic force microscopy (AFM) is appropriate for the studies of synthetic polymer molecules. (wikipedia.org)

Two-colour coincidence detection (TCCD) is a form of single-molecule fluorescence developed to sensitively detect and characterize associated biomolecules without any separation, in solution, on the cell membrane and in live cells. (silverchair.com)
More recently, single-molecule fluorescence is the subject of intense interest for biological imaging, through the labeling of biomolecules such as proteins and nucleotides to study enzymatic function which cannot easily be studied on the bulk scale, due to subtle time-dependent movements in catalysis and structural reorganization. (wikipedia.org)
Single-molecule sensing technologies aim to detect and characterize single biomolecules, but generally need labeling while the measurement times and throughput are severely restricted by a lack of positional control over the molecule. (tudelft.nl)

Carbyne - a straight line of carbon atoms linked by double bonds or by alternating single and triple bonds - is the next stiff, carbon-based structure with unusual and desirable properties. (foresight.org)
Built in a lab from individual atoms, single molecule junctions can be carefully designed and manipulated to create precise, desired effects in nanoscale electronics. (nanowerk.com)
Atoms and molecules excited with short intense laser pulses are ionized by single or multiphoton processes. (lu.se)

In our previous work using single-molecule FRET and MD simulation, we have shown that the monomer of Aβ42 is almost completely disordered with no structured regions that could possibly be a template for fibril formation 11 . (biorxiv.org)

Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. (cdc.gov)

Fundamental Studies of Enzymes: From Single Molecule Kinetics to Conformation Studies. (tufts.edu)
Single-cell kinetics of siRNA-mediated knockdown in cells expressing destabilized eGFP unveiled a. (lu.se)
Single-cell kinetics of siRNA-mediated knockdown in cells expressing destabilized eGFP unveiled a dose-response relationship with respect to knockdown induction, depth and duration in the range from several hundred to thousands of cytosolic siRNA molecules. (lu.se)

This temperature reduction allows hybridization to occur between the deoxyadenosine region on the capture oligomer and the poly-deoxythymidine molecules that are covalently attached to the magnetic particles. (cdc.gov)

These investigations open up a new route that is able to be immediately applied to probe fast single-molecule physics or biophysics with high time resolution, making an important contribution to broad fields beyond reaction chemistry. (escholarship.org)
The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of single molecule technologies. (cshlpress.com)

For the first time, real-time single-molecule electrical measurements unambiguously show reproducible large-amplitude two-level fluctuations that are highly dependent on solvent environments in a nucleophilic addition reaction of hydroxylamine to a carbonyl group. (escholarship.org)
Single-molecule studies may be contrasted with measurements on an ensemble or bulk collection of molecules, where the individual behavior of molecules cannot be distinguished, and only average characteristics can be measured. (wikipedia.org)
The method examined thousands of current-voltage measurements taken across junctions made with three different molecules. (nanowerk.com)
understand the differences between ensemble measurements and single-molecule assays. (tum.de)
It turns out that the single-molecule conductance falls off sharply with the length of the molecule so that only extremely short stretches of DNA are useful for electrical measurements. (innovationtoronto.com)

However, the idea of measuring conductance to look at single molecules placed a serious limitation on the kind of systems which could be observed. (wikipedia.org)
This is because the adsorbed molecule generated a trap state in the carbon nanotube, which changed its conductance. (nanowerk.com)
The electronic conductance of a single molecule junction - in other words, how easy it is for an electric current to pass through it - is highly sensitive to contact between the metal electrodes and single molecules, and can also be affected by different molecular conformations. (nanowerk.com)
placing the molecule on top or between electrodes reduced conductance. (nanowerk.com)
The ease with which an electric current can flow through the junction - its conductance - is highly sensitive to tiny changes caused by the contact between the molecule and the metal electrodes, for example. (nanowerk.com)
As such, scientists have been trying to find ways of generating detailed pictures of single molecule junctions, how they respond to changes in voltage and current and how their molecular structure at the interface with the electrodes influences their conductance ability. (nanowerk.com)
C4DA, the only molecule with a flexible backbone (the other two were rigid), displayed significant current fluctuation with a number of conductance band structures dependent on the shape of the molecule and its coupling (binding) strength. (nanowerk.com)
In addition, the researchers showed how the position of a molecule and the electrodes can alter a junction's conductance. (nanowerk.com)
In junctions created with a BDT molecule, the conductance was at its greatest when the molecule was placed in one of three 'bridging' positions (see image) - other conformations at the metal-molecule interface produced lower conductance. (nanowerk.com)
To their delight, they found that that the single-molecule junction with the long 90-mer DNA showed an unprecedented high conductance. (innovationtoronto.com)

We present a reliable, label-free single-molecule approach that allows us to directly explore the dynamic process of basic chemical reactions at the single-event level by using stable graphene-molecule single-molecule junctions. (escholarship.org)
These junctions are constructed by covalently connecting a single molecule with a 9-fluorenone center to nanogapped graphene electrodes. (escholarship.org)
Nanowerk News ) Scientists at Tokyo Institute of Technology have developed a statistical method that obtains detailed descriptions of metal-molecule interfaces in single molecule junctions and verifies how molecular conformation can influence their performance ( 'Resolving metal-molecule interfaces at single-molecule junctions' ). (nanowerk.com)
Yuki Komoto, Shintaro Fujii, and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have developed a statistical approach that generates detailed pictures of the metal-molecule interface in these junctions. (nanowerk.com)
The approach generates a correlated statistical description of both current and voltage characteristics, which provides useful details about molecule-electrode coupling and associated energy differences within the junctions. (nanowerk.com)
The researchers hope their method will enable scientists to visualize the details of molecule-metal interfaces in single molecule junctions, and help enhance junction performance. (nanowerk.com)
However, it is incredibly challenging to monitor the molecular-level interactions that occur between the single molecules and the other components in these junctions. (nanowerk.com)
It will also enhance theoretical knowledge of how single molecule junctions perform. (nanowerk.com)
scientists have established that the intricate structures of DNA have made it possible for it to be used in new-age electronic devices with junctions comprising just a single DNA molecule. (innovationtoronto.com)

Here, we report a surface enhanced Raman scattering technology for rapid and label-free exosomal detection (Exo-SERS) to aid in the discrimination of different cancer cells based on specific Raman phenotypes and multivariate statistical analysis. (mdpi.com)
In combination with this is the opportunity to use tip-enhanced Raman scattering (TERS) which provides very high spatial resolution as well as potentially single molecule detection. (rsc.org)
Surface-enhanced resonance raman scattering: single-molecule detection in a Langmuir-Blodgett monolayer. (bvsalud.org)

Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin-biotin interaction to be used for the development of robust and highly sensitive assays. (genomicglossaries.com)

Quantum dots are semiconducting, nanoscale clusters that show electronic characteristics distinct from both bulk-scale materials and single molecules. (foresight.org)
A Japanese collaboration led by Osaka University has explored the ability of single molecules to affect the noise generated by carbon nanotube-based nanoscale electronic devices ( 'Room-temperature discrete-charge-fluctuation dynamics of a single molecule adsorbed on a carbon nanotube' ). (nanowerk.com)
Single molecules are now doing the job of larger components in nanoscale electrical circuits. (nanowerk.com)
An optimistic Dr. Nishino speculates, "The strategy presented in our study could provide a basis for innovations in nanoscale electronics with superior designs of single-molecule electronics that could likely revolutionize nanobiotechnology, medicine, and related fields. (innovationtoronto.com)

These carbon nanotube-based devices illustrate that it is possible to detect single molecules through their unique noise signatures in the device current signals. (nanowerk.com)
How do we detect single molecules at Chemical Physics? (lu.se)

It was found that different molecules gave unique noise signals related to the properties of the molecules. (nanowerk.com)

Progenity has developed a strong patent portfolio supporting its single-molecule detection platform, including six distinct patent families that cover different aspects of the company's molecular counting platform and a separate family directed to detecting nucleosomal positioning patterns, which can be useful for determining cell-free DNA tissue-of-origin. (bioratherapeutics.com)
BOSTON) - As medical science has come to understand that the human body is controlled on the molecular level by various proteins, hormones, drugs, and other substances, technologies have developed to detect levels of these molecules in order to monitor health and diagnose disease. (harvard.edu)
The broader impact / commercial potential of this Small Business Innovation Research (SBIR) Phase I Project is to reduce time-to-diagnosis for COVID-19 through the development of a novel all-electronic single-molecule molecular diagnostics platform. (sbir.gov)
Here, microfluidic technology is used to probe the molecular heterogenity of single circulating tumor cells so as to obtain patient derived information for the personalized treatment of cancer patients. (selectbiosciences.com)
As molecular and cellular biologists move toward nano techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. (cshlpress.com)
Single-nucleotide polymorphism typing analysis for molecular subtyping of Salmonella Tennessee isolates associated with the 2007 nationwide peanut butter outbreak in the United States. (cdc.gov)
Specific molecular analysis is made by single-strand confirmation polymorphism (SSCP), direct DNA analysis, denaturing gradient gel electrophoresis, or reverse transcriptase-polymerase chain reaction to search for the BTK mutation. (medscape.com)

The ability to characterize single molecules using highly sensitive nanoelectronics is an exciting prospect in the field of sensors, particularly for neuro- and biosensor applications. (nanowerk.com)

11] P. Ghenuche, J. de Torres, P. Ferrand, J. Wenger, Multi-focus parallel detection of fluorescent molecules at picomolar concentration with photonic nanojets arrays, Appl. (fresnel.fr)

The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)

No evidence was obtained for of Chemistry, Lund University water molecules bound with longer residence times. (lu.se)

Here we present a multifunctional anchor, an acrylate epoxide, that enables proteins and RNAs to be equipped with anchors in a single experimental step. (plos.org)
Through single-molecule techniques the step mechanism has been observed and characterized in many of these proteins. (wikipedia.org)
To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immune complexes (one or zero labeled target protein molecules per bead) with an enzymatic reporter capable of generating a fluorescent product. (quanterix.com)
We recently introduced the nanopore electro-osmotic trap (NEOtrap) which can trap and sense single unmodified proteins for long times. (tudelft.nl)
The current results might open a new research field of investigating proteins based on the assimilation of data obtained by single molecule method and MD calculation. (nii.ac.jp)

Viable single molecule FCS experiments at concentrations 1-1000 nM with different objectives costing less than $40 are demonstrated. (fresnel.fr)
The first single-molecule experiments were patch clamp experiments performed in the 1970s, but these were limited to studying ion channels. (wikipedia.org)
In the gas phase at ultralow pressures, single-molecule experiments have been around for decades, but in the condensed phase only since 1989 with the work by W. E. Moerner and Lothar Kador. (wikipedia.org)
Nanomanipulators such as the atomic force microscope are also suited to single-molecule experiments of biological significance, since they work on the same length scale of most biological polymers. (wikipedia.org)
The location of two-chain-superposition correspond in these experiments to twice the thickness of single chain (0.8 nm in the case of the mentioned example). (wikipedia.org)
At the application of proper scanning parameters, conformation of such molecules remain unchanged for hours that allows the performance of experiments under liquid media having various properties. (wikipedia.org)
In Chapter 5 we performed single molecule experiments on the diferent β-glucuronidase mutants. (tufts.edu)
Single-Molecule Techniques: A Laboratory Manual fills this requirement it is the first to take researchers who know nothing about single molecule analyses to the point where they can successfully design and execute appropriate experiments. (cshlpress.com)

This realizes a novel regime where several focal spots are illuminated to detect the possible presence of a fluorescent molecule in one of them. (fresnel.fr)

Our plan is to use this method in diagnostics for improved detection of hormones in blood samples," said first author Xu Wang, Ph.D., a Postdoctoral Research Fellow at BWH and the Wyss Institute. (harvard.edu)
By sensing molecules previously undetectable within an hour, they open entirely new approaches to diagnostics and clinical monitoring that should greatly improve human health. (harvard.edu)
An important basis of this work is the Swedish Research Council Research Environment on Single molecule bioanalytical sensing for precision cancer diagnostics funded 2020 - 2025. (lu.se)

A reaction then takes place that makes any well containing a bead with the labeled target molecule fluoresce. (harvard.edu)
In wells containing a bead with a single target molecule, the formation of an immunocomplex will result in enzymatic cleavage of a substrate, which will be converted to a fluorescent product imaged by the SR-X reader. (lu.se)

Bionano sells a DNA mapping system for structural variation detection called Saphyr that it launched commercially in 2017. (genomeweb.com)
The potential of DNA structural properties in single-molecule electronics has finally been harnessed by researchers in a single-molecule junction device that shows spontaneous self-restoring ability. (innovationtoronto.com)

Single-molecule detection can reveal time trajectories and reaction pathways of individual intermediates/transition states in chemical reactions and biological processes, which is of fundamental importance to elucidate their intrinsic mechanisms. (escholarship.org)
After isolating the beads in 50-fl reaction chambers designed to hold only a single bead, we used fluorescence imaging to detect single protein molecules. (quanterix.com)
Multi-color imaging of polymer growth by single catalysts at single-monomer resolution has been realized through CREATS (coupled reaction approach toward super-resolution-imaging). (rit.edu)
The approach is based on the counting of thousands of single immunocomplexes in femtoliter-sized reaction chambers which are termed Simoa - Single Molecule Arrays. (lu.se)

By analyzing the amount of the labeled molecule of interest that is bound to the antibodies compared to the total number of available antibody sites, it is possible to conclude that the remaining sites are bound by the unlabeled molecule from the sample, allowing the concentration of that molecule to be determined. (harvard.edu)
After being sealed into the microwells on a Simoa plate, any beads that are bound to a labeled version of the target molecule glow fluorescently (green), while beads that contain an unlabeled target molecule remain dark. (harvard.edu)
After the bead/antibody mixtures are allowed to mix with a sample containing an unknown amount of the target molecule, the beads are rinsed to remove any unbound molecules and then added to a Simoa disc containing thousands of microwells, each of which can hold one bead bound to one target molecule. (harvard.edu)

This session will set out some of the latest breakthroughs in using SERS for biological applications including immunoassays and ultra sensitive biomolecular detection. (rsc.org)

At the same time, advancements in analytical chemistry allowed for the detection of trace amounts of chemicals in tissues, often in the range of parts per billion. (cdc.gov)

The strength of the interaction between the carbon nanotubes and molecules was able to be predicted from the obtained noise signals. (nanowerk.com)
This gives us unprecedented opportunities to study the physics of confined polymers, the interaction between DNA and other molecules and the genomics of individual DNA molecules. (lu.se)

Single-molecule protein identification by sub-nanopore sensors. (cdc.gov)

The system detects single particulate impurities. (fraunhofer.de)
MLST is well-suited for the analysis of wastewater RNA because it detects a set of mutations unique to a variant and does not require these mutations to be present on a single molecule of RNA ( 12 - 18 ). (cdc.gov)

current detection approach of studying single enzymes. (tufts.edu)
The heating platform allows us to study single enzymes with respect to temperature variations. (tufts.edu)
We showed that the activation energy greatly distributes along the molecules and enzymes with different thermal stabilities might be present within the population. (tufts.edu)
By using the heating platform we demonstrate that at the single molecule level a fraction of the individual enzymes behave as thermal switches, where they lack activity at room temperature but become active when treated with a heating pulse. (tufts.edu)

Fig. 6: Dependence of SV detection accuracy on the level of coverage. (nature.com)
Finally Chapter 6 describes the thermal denaturation of monomeric enzyme and refolding studies performed on the single molecule level. (tufts.edu)
In this talk, I will present synthesis of small fluorescent monomers with different emission colors, and polymerization of synthesized monomers at both ensemble and single-molecule level. (rit.edu)
The team used a 10-mer and a 90-mer DNA strand (which indicate the number of nucleotides, basic building blocks of DNA, comprising the molecule length) to form a zipper-like structure and attached them to either a gold surface or to the metal tip of a scanning tunneling microscope, an instrument used to image surfaces at the atomic level. (innovationtoronto.com)
To study processes at the level of single molecules and single cells, the tools need to be on the same size scale as the objects under study. (lu.se)

We have achieved real-time optical sequencing of single synthetic polymer chains under living polymerization conditions. (rit.edu)

The underlying optical mapping technology involves nanochannels that hold single DNA molecules. (genomeweb.com)
DNA confined in nanochannels naturally stretches out due to a combination of excluded volume effects and elastic properties of the DNA molecule depending on the size of the channels. (lu.se)

Despite the availability of efficient treatment, controlling the spread of TB remains a challenging task, primarily due to the lack of fast and sensitive detection technology. (cdc.gov)

2Condensed Matter Magnetic indicate that the hydration water molecules exchange with bulk water on Resonance Group, Department the sub-nanosecond time scale at 4C. (lu.se)

As part of a program in the Analytical Biotechnology of single molecules, we are developing and exploiting variants of these strategies in a variety of areas. (dbkgroup.org)
SAN DIEGO, Dec. 02, 2021 (GLOBE NEWSWIRE) -- Progenity, Inc. (Nasdaq: PROG), a biotechnology company innovating in the fields of gastrointestinal health and oral biotherapeutics, today announced a new patent related to its single-molecule detection platform under development. (bioratherapeutics.com)

This method enables detection and quantification of sub-nanomolar cytosolic siRNA release amounts from individual release events with measures of quantitation confidence for each event. (lu.se)

What this means is that the carbon nanotube-based devices were so sensitive that the researchers were able to detect unique signature from single molecules. (nanowerk.com)
For both BDA and C4DA, the researchers simulated one single interfacial configuration, but for BDT they resolved three different interfacial arrangements. (nanowerk.com)

Single cell protein analyses provide important insights in cellular heterogeneity. (selectbiosciences.com)

The ability to detect single protein molecules 1 , 2 in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. (quanterix.com)
They analyzed data from the Alzheimer's Disease Neuroimaging Initiative, a multicenter study designed to identify biomarkers for the detection and tracking of AD. (medscape.com)

To demonstrate the detection and differentiation of arginine PTMs, we applied our platform to distinguish between not only asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) but also between citrulline and native arginine residues. (quantum-si.com)
These mutations affect critical residues in the cytoplasmic BTK protein and are highly variable and uniformly dispersed throughout the molecule. (medscape.com)

This showed that the single-molecule junction was both resilient and easily reproducible. (innovationtoronto.com)

The signal generated by the carbon nanotube device changed following the adsorption of specific single molecules," says first author Agung Setiadi. (nanowerk.com)

Although much of our work is relevant to modern industrial technology, most of it is basic research which aims to answer basic questions about the nature and behaviour of surfaces and molecules. (lu.se)

Anatomy10

Organisms10

Chemicals and Drugs74

Analytical, Diagnostic and Therapeutic Techniques and Equipment77

Psychiatry and Psychology1

Phenomena and Processes75

Disciplines and Occupations14

Technology, Industry, Agriculture17

Information Science12

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Sensitivity14

Fluorescence Detection2

Methods10

Spectroscopy7

Target molecules5

Assay5

Nanoparticles2

Individual molecules5

Excitation2

Microscopy1

Biomolecules3

Atoms3

FRET1

Nucleic acids1

Kinetics3

Particles1

Biophysics2

Measurements5

Conductance10

Junctions9

Raman3

Assays1

Nanoscale4

Detect single molecules2

Different molecules1

Molecular7

Characterize1

Fluorescent molecules1

Probe2

Lund University1

Proteins5

Experiments8

Illuminated to detect1

Diagnostics3

Containing a bead2

Structural2

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Biological1

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