Monoclonal antibodiesConjugatesAntigenRecombinantAntigensEnzyme-linkeSpecificityHybridomaRelates to methodsTherapeuticBispecificPeptideBioanalysisIsotypeReagentsCytotoxicityReactiveLigandsPolyclonalEpitopeAutoimmuneAutoantibodiesImmunogenicityDiscoveryResiduesDetectIdiotypeVitroVariantsTargetsLymphocytesScFvMammalianDownstreamEvaluationCancer therapySpecificFragmentImmune responseBoundCloneTherapySignificantlyDevelopmentCellsRatsDrugStagesHuman
Monoclonal antibodies5
- In contrast to monoclonal antibodies produced by hybridoma technology, which may lose the capacity to produce the desired antibody over time or the antibody may undergo unwanted changes, which affect its functionality, recombinant antibodies produced in phage display maintain high standard of specificity and low immunogenicity. (wikipedia.org)
- These molecules are comprised of monoclonal antibodies conjugated with a highly cytotoxic agents (aka payload) via synthetic linkers. (betasciencepress-publishing.com)
- Thorpe, (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications, A. Pinchera et al (ed.s), pp. 475-506). (justia.com)
- 21:183-87) and monoclonal antibodies (mAbs) as well as drug-linking and drug-releasing properties (Lambert, J. (2005) Curr. (justia.com)
- the system is designed to identify and type hsv from direct lesion smears (ds) and from cell culture smears (cs) infected with a swab from the patient's lesion by means of an indirect fluorescence assay (ifa) using hsv type-common and hsv-2 type-specific monoclonal antibodies. (liverpool.ac.uk)
Conjugates6
- Antibody-drug conjugates (ADCs) are multicomponent biotherapeutic drugs designed primarily for cancer therapy. (betasciencepress-publishing.com)
- Cysteine engineered anti-MUC16 antibodies (Ab) are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered anti-MUC16 antibody-drug conjugates having Formula I: Ab-(L-D)p I where p is 1 to 4. (justia.com)
- The use of antibody-drug conjugates (ADC), i.e. immunoconjugates, for the local delivery of cytotoxic or cytostatic agents, i.e. drugs to kill or inhibit tumor cells in the treatment of cancer (Lambert, J. (2005) Curr. (justia.com)
- Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
- For this purpose, we synthesized carcinogen-protein conjugates and prepared mono- and polyclonal antibodies to benzo[a]pyrene. (actanaturae.ru)
- Antibody-toxin conjugates (immunotoxins) can be produced by using conventional chemical coupling techniques or recombinant DNA techniques. (creativebiolabs.net)
Antigen15
- scFv is the smallest of the recombinant antibody formats, which is capable of antigen binding. (wikipedia.org)
- Bispecific antibodies combine two different antigen binding specificities within one molecule. (wikipedia.org)
- Anti-idiotypic antibody mimicking a T-antigen-specific lectin inhibits human epithelial tumor cell proliferation. (conicet.gov.ar)
- The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. (justia.com)
- In one aspect of the invention, a method is provided for determining the sequence of an antibody and its respective binding partner, e.g., antigen such as an autoantigen. (justia.com)
- The captured antibodies in one or more of the containers are exposed to an antigen display library comprising a plurality of display particles for a time sufficient to allow for interaction of one or more of the captured antibodies with the one of more of the display particles. (justia.com)
- Identification of such tumor-associated cell surface antigen polypeptides, i.e. tumor associated antigens (TAA), allows specific targeting of cancer cells for destruction via antibody-based therapies. (justia.com)
- Independent of antibody secretion B cells secrete proinflammatory cytokines, support the formation of ectopic GCs, and serve as antigen presenting cells. (hindawi.com)
- c) Effect of antibodies and antigen-specific B cells on antigen uptake. (hindawi.com)
- Left panel: antigen bound by antibody is taken up via FcR on APCs such as dendritic cells or macrophages. (hindawi.com)
- Likewise, antigen/antibody complexes are bound by the FcR of APCs and processed in a unique fashion dependent on the epitope specificity of the bound antibody. (hindawi.com)
- The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
- This leaves the antigen-binding domains fully accessible to enable the most effective capture of desired target (Figure bellow). (biaseparations.com)
- hsv specific antibody production was detected in culture supernatants of pbmc from hsv seropositive healthy individuals by stimulating with optimal concentrations of hsv antigen without mitogens. (liverpool.ac.uk)
- The antibody transports the toxin in the body and selectively targets it to tumour cells by binding to a cell-surface antigen. (creativebiolabs.net)
Recombinant12
- Recombinant antibodies are antibody fragments produced by using recombinant antibody coding genes. (wikipedia.org)
- Recombinant antibodies have many advantages in both medical and research applications, which make them a popular subject of exploration and new production against specific targets. (wikipedia.org)
- There are several known formats of recombinant antibodies which are commonly produced. (wikipedia.org)
- These are the Fab recombinant antibodies, scFv and diabodies. (wikipedia.org)
- The functionality may be enhanced by site-specific chemical modifications, adding a peptide-tag or by fusion with a gene to achieve production of bifunctional recombinant antibodies. (wikipedia.org)
- As a member of the anti-idiotypic antibodies, Fab fragment recombinant antibodies bind directly to the paratope of the target antibody. (wikipedia.org)
- Along scFv and Fab fragments, diabodies or bispecific recombinant antibodies are the third major format. (wikipedia.org)
- The production of recombinant antibodies follows principally similar workflow. (wikipedia.org)
- Once the construct is delivered to the laboratory, expression constructs are produced, then they are transferred to a cell culture in the process called transfection and once the cell culture produces the desired recombinant antibody, it is regularly collected, purified and analyzed or used for further experimentation. (wikipedia.org)
- For recombinant antibody production the stable cell lines such as CHO and HEK293 are used. (wikipedia.org)
- In the beginning phases of the recombinant antibody production it was important to achieve the assembly of a functional Fv fragment in Escherichia coli. (wikipedia.org)
- Using ELISA, we demonstrated that the recombinant protein specifically interacts with the anti-benzo[a]pyrene monoclonal antibody (mAB B2). (actanaturae.ru)
Antigens1
- In diseases and conditions in which autoantibodies are generated (e.g., autoimmune disease, cancer), the adaptive immune system escapes "tolerance", the internal checks and balances that would normally prevent the generation of antibodies that bind to self-antigens, and results in chronic inflammation, fibrosis, progressive tissue damage or a combination of the foregoing. (justia.com)
Enzyme-linke1
- an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. (liverpool.ac.uk)
Specificity1
- Based on their binding specificity 3 types of anti-idiotypic antibodies can be distinguished, which partially overlap with the previously mentioned formats: the classical ones, a group including Fab fragment antibodies, antibodies binding to idiotope outside of the drug binding site and antibodies, which only bind to the already assembled complex of drug bound to the target. (wikipedia.org)
Hybridoma1
- Methods for identifying such antibodies have been previously described and include hybridoma approaches, display library approaches, B-cell immortalization, B-cell cloning, and single cell screening methods. (justia.com)
Relates to methods1
- The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions. (justia.com)
Therapeutic3
- Diagnostic and therapeutic uses for cysteine engineered antibody drug compounds and compositions are disclosed. (justia.com)
- The invention relates generally to antibodies engineered with reactive cysteine residues and more specifically to antibodies with therapeutic or diagnostic applications. (justia.com)
- We currently have 3 therapeutic antibodies already on the market and more than 30 in preclinical and clinical stages. (proteogenix.science)
Bispecific2
- The most commonly used are the scFv, Fab fragments and bispecific antibodies. (wikipedia.org)
- The bispecific antibodies are used to crosslink the target molecules with two different cells and mediate direct cytotoxicity. (wikipedia.org)
Peptide1
- In fact, a generic hybrid LC-MS/MS method utilizing a generic immunocapture capture reagent and a universal peptide from humanized monoclonal antibody, typically from the Fc region, can be utilized for bioanalysis of antibody-based biotherapeutics with a Fc backbone for preclinical applications [4]. (betasciencepress-publishing.com)
Bioanalysis1
- Quantitative bioanalysis of antibody-based biotherapeutics by LC-MS is typically conducted using Immunoaffinity (IA) enrichment coupled with liquid chromatography and tandem mass spectrometry (LC-MS/MS), also known as (aka) hybrid LC-MS/MS [3]. (betasciencepress-publishing.com)
Isotype5
- Setting the corresponding isotype control antibody to detect non-specific binding can reduce the generation of false positive results and evaluate the possible influencing factors accurately in the drug development process. (acrobiosystems.com)
- Figure A. Alexa Fluor 647 conjugated Mouse anti Rat CD54 ( MCA773A647 ) and FITC conjugated Mouse IgG1 isotype control ( MCA1209F ). (bio-rad-antibodies.com)
- Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 ( MCA1940A488 ) and Pacific Blue conjugated Mouse IgG1 isotype control ( MCA928PB ). (bio-rad-antibodies.com)
- Figure A. RPE conjugated Mouse anti Human CD19 ( MCA1940PE ) and Pacific Blue conjugated Mouse IgG2a isotype control ( MCA929PB ). (bio-rad-antibodies.com)
- Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 ( MCA1940A488 ) and RPE-Alexa Fluor 647 conjugated Mouse IgG2a isotype control ( MCA929P647 ). (bio-rad-antibodies.com)
Reagents1
- Generic immunocapture reagents such as Protein A or G beads, anti-human Fc or anti-human IgG antibodies can be utilized in a hybrid LC-MS/MS for preclinical evaluations [4]. (betasciencepress-publishing.com)
Cytotoxicity1
- Binding of the Fc portion of the antibodies to FcR leads to activation of effector cells and further release of proinflammatory cytokines and proteolytic enzymes, mediators of antibody-dependent cell-mediated cytotoxicity (ADCC). (hindawi.com)
Reactive2
- Cysteine engineered anti-MUC16 antibodies are engineered by replacing one or more amino acids of a parent anti-MUC16 antibody with non cross-linked, reactive cysteine amino acids. (justia.com)
- Pre-stimulation ex vivo of allogeneic T cells by exposure to MOPC315.BM MM cells in the presence of IL-2, anti-CD3 and anti-CD28 resulted in expansion of the myeloma-reactive T cell TCRVβ 2, 3 and 8.3 subfamilies. (biomedcentral.com)
Ligands3
- The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
- Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
- Immunoaffinity columns using antibodies as ligands against mammalian proteins could be used for different applications in protein expression control and, if a standard available, for direct protein quantification in complex sample solutions. (biaseparations.com)
Polyclonal2
- GenScript ProBio has experience in developing of highly sensitive and specific polyclonal and monoclonal anti-ID Abs to support your antibody drug development. (genscriptprobio.com)
- A Human Monoclonal Antibody Against Domain I Of beta 2-Glycoprotein I prevents Clotting and Fetal Loss Induced By Polyclonal Anti-Phospholipid Antibodies In Animal Models. (units.it)
Epitope3
- The adaptive immune response produces a diversity of different antibodies (on the order of 10 11 unique sequences in a single individual), each of which is capable of binding to a different target epitope. (justia.com)
- The isolation of antibodies that bind to a specific target, let alone a specific epitope on a known target, is of high interest for the development of new products in therapeutics, diagnostics, and more generally, for research purposes. (justia.com)
- A much more challenging problem in antibody discovery is presented when it is known, or suspected, that an antibody response is present and is biologically relevant, but the identity of the target (and consequently, target epitope) is not known, and could be a single target selected from hundreds to tens of thousands of different targets. (justia.com)
Autoimmune1
- Through these mechanisms B cells are involved both in autoimmune diseases that are traditionally viewed as antibody mediated and also in autoimmune diseases that are commonly classified as T cell mediated. (hindawi.com)
Autoantibodies1
- Further, even in the case of two patients that make autoantibodies against the same target, the sequences of these antibodies will be different. (justia.com)
Immunogenicity1
- Due to the similarity between anti-ID Abs and anti-drug antibodies, anti-ID Abs are also commonly used as a reference standard for antibody drug immunogenicity, anti-drug antibody (ADA) studies and neutralization antibody measurement. (genscriptprobio.com)
Discovery1
- One-stop service from anti-ID Ab discovery to PK and ADA kit development and manufacturing, covering preclinical PK and ADA testing needs once for all. (genscriptprobio.com)
Residues1
- They take advantage of a special hydrazide linkage that binds antibodies through the carbohydrate residues on their Fc regions. (biaseparations.com)
Detect1
- Type 1 or type 2 could be applied to detect antibody drug when having a cell-bound drug target e.g. (genscriptprobio.com)
Idiotype2
- An anti-idiotypic antibody (anti-ID Ab, see the schematic diagram ) binds to the idiotype of another antibody, usually an antibody drug. (genscriptprobio.com)
- An idiotype can be defined as the specific combination of idiotopes present within an antibodies complement determining regions. (genscriptprobio.com)
Vitro3
- 5-(2-fluoroethyl)-2'-deoxyuridine (fedu), its 2'-fluoroarabinofuranosyl analog (fefau) and the 2'-fluoroarabinofuranosyl analog (cefau) of the potent anti-herpesvirus compound 5-(2-chloroethyl)-2'-deoxyuridine (cedu) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro and in vivo. (liverpool.ac.uk)
- fedu, fefau and cefau proved to be potent and selective anti-herpesvirus agents in vitro. (liverpool.ac.uk)
- limiting dilution analysis of specific in vitro anti-herpes simplex virus antibody production by human lymphocytes. (liverpool.ac.uk)
Variants2
- Antibody charge variants have gained considerable attention in the biotechnology industry due to their potential influence on stability and biological activity and cation-exchange chromatography (CEX) is one of the typical approaches for mAb charge variant analyses. (biaseparations.com)
- Benefit from the most productive transient expression system to produce all your humanized monoclonal antibody variants! (proteogenix.science)
Targets2
- Transmembrane or otherwise tumor-associated polypeptides specifically expressed on the surface of cancer cells as compared to normal, non-cancerous cell(s) have been identified as cellular targets for cancer diagnosis and therapy with antibodies. (justia.com)
- The data strongly indicate that CS-GAGs and their biosynthetic pathways are promising targets for the development of anti-metastatic therapies. (biomedcentral.com)
Lymphocytes1
- The method entails optionally subjecting a sample to conditions suitable for expansion of one or more of the lymphocytes to form an expanded sample comprising antibody secreting cells (ASCs) and distributing the sample into containers to provide a plurality of subsamples. (justia.com)
ScFv1
- The most commonly used form is the single chain variable fragment (scFv), which has shown the most promising traits exploitable in human medicine and research. (wikipedia.org)
Mammalian1
- Optimizations of mammalian cell cultures have led to increase the yield of antibodies from HEK293 or CHO cell lines to over 12g/liter. (wikipedia.org)
Downstream3
- We try to work as closely as possible with our customers to understand their specific downstream application in order to generate their perfect anti-ID. (genscriptprobio.com)
- Most importantly, such columns could extract a significant amount of a single membrane protein from native source, suitable for downstream analyses, such as mass spec analysis of their glycans. (biaseparations.com)
- The upstream and downstream monoclonal antibody (mAb) bioprocessing makes them susceptible to physical and chemical modifications. (biaseparations.com)
Evaluation1
- evaluation of a monoclonal antibody typing system for herpes simplex virus. (liverpool.ac.uk)
Cancer therapy1
- Polakis, P. Arming antibodies for cancer therapy. (justia.com)
Specific1
- Anti-idiotypic antibodies bind to a paratope of another specific antibody. (wikipedia.org)
Fragment2
- Fab fragment antibodies can be used for detection of not bound drugs or free drugs in the serum. (wikipedia.org)
- Fab antibodies have also been used to avoid the adverse effects caused by unspecific binding of the Fc portion of the antibody, which is missing in the Fab fragment. (wikipedia.org)
Immune response1
- Results Vaccinated dogs developed anti-Do-CSPG4 and Hu-CSPG4 immune response. (bmj.com)
Bound1
- The Fc portion of antibodies in immune complexes can be bound by C1q of the classical complement pathway, which eventually leads to the release of C5a and C3a. (hindawi.com)
Clone1
- Phycoerythrin conjugated Mouse anti Rat CD161 antibody, clone 10/78 ( MCA1427PE ) used for the identification of NK cells in rat blood samples by flow cytometry. (bio-rad-antibodies.com)
Therapy1
- Antibody therapy has been established for the targeted treatment of patients with cancer, immunological and angiogenic disorders. (justia.com)
Significantly2
- Interestingly, the antibody titer in vaccinated dogs was significantly associated with the overall survival. (bmj.com)
- Anti-SRBC antibody titres were significantly higher in operated animals than in controls. (shengsci.com)
Development1
- Another researched possibility is the development of anti-idiotypic antibodies. (wikipedia.org)
Cells2
- B cells have antibody-dependent and antibody-independent pathogenic functions. (hindawi.com)
- The effect of a standard laparatomy on antibody synthesis was studied in Wistar R/A rats recieving an intravenous injection of 10(9) sheep red blood cells (SRBC) during the surgical procedure. (shengsci.com)
Rats2
- A: 3-week-old (young) Lewis rats were injected twice with a NK cell depleting antibody (3.2.3). (bio-rad-antibodies.com)
- Stimulation of antibody synthesis induced by surgical trauma in rats. (shengsci.com)
Drug1
- An immunotoxin is a macromolecular drug which consists of a monoclonal antibody linked to a protein toxin.The toxin is usually a cytotoxic protein derived from a bacterial or plant protein, such as Diphtheria toxin (DT), Cholera toxin (CT), Trichosanthin (TCS), Dianthin etc. (creativebiolabs.net)
Stages1
- The control group showed negative or low staining when stained with PHF-1, an antibody that recognizes the early stages of a NFT. (mi-rna.com)
Human2
- Figure B. FITC conjugated Mouse anti Human CD19 ( MCA1940F ) and Pacific Blue conjugated Mouse anti Human HLA ABC ( MCA81PB ). (bio-rad-antibodies.com)
- Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 ( MCA1940A488 ) and RPE-Alexa Fluor 647 conjugated Mouse anti Human HLA ABC ( MCA81P647 ). (bio-rad-antibodies.com)