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  • Abstract
  • article{c58957be-5df1-4efc-b433-f02e50dc2d3f, abstract = {CD40 plays a central regulatory role in the immune system and antibodies able to modulate CD40 signalling may consequently have a potential in immunotherapy, in particular for treatment of lymphomas and autoimmune disease like multiple sclerosis. (lu.se)
  • molecule
  • Second, a family of anti-fluorescein antibodies and their binding to the small molecule fluorescein-biotin were explored. (mit.edu)
  • Chloramine T), displayed a binding ability extremely similar to that of the parental monovalent Fab' fragment, as well as a flow cytometry performance and an equilibrium binding affinity (Ka approximately 2 × 10 8 M -1 ) only slightly lower than those of the parental bivalent antibody, suggesting that its binding site is conserved as compared to that of the parental antibody molecule. (youscribe.com)
  • With greater understanding of these obstacles and the ability to engineer changes within the antibody molecule itself, there has been renewed enthusiasm for development and evaluation of immunoconjugates. (springer.com)
  • affinity
  • Small and high affinity single chain antibodies (scFv) have been introduced as effective agents for cancer immunotargeting approaches. (hindawi.com)
  • High affinity antibody binding interactions are important for both pharmaceutical and biotechnological uses. (mit.edu)
  • In this Thesis, we investigate affinity maturation of antibodies for very high affinity binding. (mit.edu)
  • This study demonstrates that a single energetic component is not generally responsible for the increased affinity within a given protein-protein affinity maturation pathway. (mit.edu)
  • The femtomolar affinity matured anti-fluorescein antibody, 4M5.3, was compared to its wildtype high affinity precursor, 4-4-20. (mit.edu)
  • Single-chain antibody-based immunotoxins targeting Her2/neu: design optimization and impact of affinity on antitumor efficacy and off-target toxicity. (semanticscholar.org)
  • Affinity and avidity in antibody-based tumor targeting. (semanticscholar.org)
  • Therapeutics
  • 2 3 Thus, feasibility of topical self-administration of antibody-based therapeutics in an outpatient setting would represent a major innovation in ophthalmology. (arvojournals.org)
  • ovarian cancer
  • HER2 is highly expressed in approximately 30% patients with breast or ovarian cancer[ 1 ] and represents a potential target for antibody mediated biological therapy against cancers. (biomedcentral.com)
  • cells
  • The antibody also induced apoptosis in the PSCA expressing cells. (hindawi.com)
  • Both antibodies were further characterized by western blotting and FACS analysis: both N3 and S18 specifically recognized mouseLRP and humanLRP overexpressed in mammalian cells under denaturating conditions (western blot) and under native conditions at the cell surface (FACS). (uni-muenchen.de)
  • A particular challenge of bispecific antibody constructs that recognize the invariant CD3 signaling complex is a controlled polyclonal activation of T cells that, ideally, is exquisitely dependent on the presence of target cells. (ovid.com)
  • Glycoprotein D (gD) of bovine herpesvirus-1 (BoHV-1) is essential for attachment and penetration of cells during infection and is a major target for neutralizing antibodies during an adaptive immune response. (ebscohost.com)
  • OBJECTIVE: To develop a biochemically robust, highly reproducible, and site-specific coupling method using the Staphylococcus aureus sortase A enzyme for the conjugation of a single-chain antibody (scFv) to nanoparticles and cells for molecular imaging and cell homing in cardiovascular diseases. (edu.au)
  • An anti-CD22 scFv termed 4KB was obtained which showed the expected binding activity which was also internalized by CD22 + target cells and was also competed for by the parental monoclonal CD22 antibody. (biomedcentral.com)
  • specific
  • Based on this antibody construct, strategies for both specific tumor targeting and T cell activation are reviewed here. (jcancer.org)
  • Three strategies resulted in downregulation or blocking of LRP and prevented PrPSc accumulation in different scrapie infected neuronal cell lines (i) transfection with an antisense LRP RNA expression plasmid (ii) transfection with small interfering (siRNAs) specific for the LRP mRNA and (iii) incubation with the polyclonal anti-LRP antibody, W3. (uni-muenchen.de)
  • Currently, targeting can be achieved via chemical conjugation to specific antibodies, which typically results in the loss of antibody functionality and in severe cell damage. (edu.au)
  • Furthermore, the choice of specific radionuclide, toxin or drug conjugate has been influenced by increased knowledge of antibody internalization and processing pathways. (springer.com)
  • immunotherapy
  • Recombinant antibodies have recently shown great promise in the replacement of monoclonal antibodies in different medical areas such as immunotherapy against human malignancies [ 16 - 19 ]. (hindawi.com)
  • CD40 plays a central regulatory role in the immune system and antibodies able to modulate CD40 signalling may consequently have a potential in immunotherapy, in particular for treatment of lymphomas and autoimmune disease like multiple sclerosis. (lu.se)
  • immune
  • Horseradish peroxidase-labeled streptavidin-biotinylated scFv antibody (HRP-SA-BIO-scFv) could specifically bind to AMD-functionalized magnetic beads (MBs) and then the immune complexes were separated from the matrix solution by magnet. (springer.com)
  • Anti-CTLA-4 monoclonal antibodies activate the immune system against cancer. (rbmb.net)
  • potential
  • However, antibodies need to be delivered by (repeat) intravitreal injection, a laborious administration procedure associated with the potential for serious adverse events. (arvojournals.org)
  • These antibodies have the potential to be used for targeted therapy, where the blocking of CTLA4 receptor is needed. (rbmb.net)
  • importantly
  • Importantly, the scFv anti-VEGF antibody expressed from an AAV vector also had a significant beneficial effect (p=0.02), providing valuable preclinical data for future translation to the clinic. (soton.ac.uk)
  • detection
  • Evaluation and optimization of a commercial enzyme linked immunosorbent assay for detection of Chlamydophila pneumoniae IgA antibodies. (ebscohost.com)