Simultaneous phosphorylation regulatory phosphorylation of serine residues. Medical search. Frequent questions

Multiple posttranslational modifications (PTMs) of histone proteins including site-specific phosphorylation of serine and threonine residues govern the accessibility of chromatin. (edu.sa)
DYRKs are named after their characteristic dual-specificity, as they auto-phosphorylate a conserved tyrosine in their activation loop, but phosphorylate substrates on serine and threonine residues 2 . (nature.com)

Oxidative stress is an inescapable concomitant of the life of aerobic organisms, given that reactive oxygen species (ROS) are produced by energy generation by oxidative phosphorylation in mitochondria. (biomedcentral.com)
Cells under aerobic conditions utilize oxidative phosphorylation in mitochondria to sustain energy demands. (biomedcentral.com)

These enzymatic modifications include acetylation, methylation, phosphorylation, and ubiquitination and primarily occur at N-terminal histone tails. (wikipedia.org)
In this context, numerous histone post-translational modifications (PTMs) have been described that include acetylation, phosphorylation, methylation, ubiquitylation and SUMOylation [ 1 ]. (aging-us.com)

Phosphorylation of serines 22 and 23 in the N-terminal peptide of cardiac troponin I is responsible for lusitropy. (bvsalud.org)
There is significant rigidification of the structure involving rearrangement of the cTnI(1-33)-cTnC interaction and changes in the distribution of the cTnC helix A/B angle, troponin I (cTnI) switch peptide (149-164) docking, and the angle between the regulatory head and ITC arm domains. (bvsalud.org)
The opposite end of cardiac TnI contains a phosphorylation-sensitive â ¼30 residue-long N-terminal peptide that is absent in skeletal muscle, and likely modifies these interactions in hearts. (bvsalud.org)
Here, PKA-dependent phosphorylation of serine 23 and 24 modulates Ca2+ and possibly switch-peptide binding to TnC, causing faster relaxation during the cardiac-cycle (lusitropy). (bvsalud.org)
In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
Progesterone receptor membrane component 1 (PGRMC1) is 195 residue membrane-bound protein which contains a short luminal peptide, a single N -terminal transmembrane domain, and a C -terminal cytochrome b 5 -related heme-binding domain ( Figure 1A ). (oncotarget.com)
To better define the residues which participate in tetramer stabilization, the in vivo interaction of the BChE C-terminus 46 residue peptide was quantitated for wild type and mutant BChE using the yeast two-hybrid system. (inrae.fr)
However, only 11.7% of the interaction seen with the wild type peptide was observed with the mutant in which seven conserved aromatic residues (Trp 543, Phe 547, Trp 550, Tyr 553, Trp 557, Phe 561, and Tyr 564) had been altered to alanines (aromatics off mutant). (inrae.fr)

Our laboratory has shown that up to 40 carboxy terminal residues of each subunit contribute to the stabilization of tetramers (R.M. Blong, E. Bedows, O. Lockridge, The tetramerization domain of butyrylcholinesterase is at the carboxy-terminus, Biochem. (inrae.fr)

Similar trend was observed in MCF-7 whereby TZT treatment down-regulated the anti-apoptotic catalase and PON2, increased the proapoptotic, B cell lymphoma 2 (Bcl-2)-associated agonist of cell death (Bad) and Bcl-2-associated X (Bax), second mitochondria-derived activator of caspase (SMAC) and HtrA serine peptidase 2 (HTRA2) as well as TRAIL receptors (TRAIL R1 and TRAIL R2). (eurekaselect.com)
In addition the regulation of these receptors by phosphorylation and the consequences this may have for Ca2+ signaling are also being studied. (rochester.edu)
Regulatory proteins that down-regulate phosphorylated G-protein membrane receptors, including rod and cone photoreceptors and adrenergic receptors. (lookformedical.com)
A family of serine-threonine kinases that are specific for G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)

Homeodomain-interacting protein kinases (HIPKs) belong to the CMGC kinase family and are closely related to dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs). (nature.com)
HIPKs belong to the CMGC group of serine/threonine kinases and are part of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. (nature.com)
C-terminally adjacent to the HID follows a proline, glutamate, serine, and threonine (PEST)-rich domain, mediating proteasomal degradation of these kinases. (nature.com)

In a preferred embodiment, a GEF-H1 polypeptide is provided that lacks the amino acid region between residues 162 and 354 of SEQ ID NO. 2. (justia.com)
Figure 1: PGRMC1 is phosphorylated on key regulatory amino acid residues. (oncotarget.com)
The positions of highlighted regulatory phosphorylations observed in (B) below are indicated, as are the amino acids included in the NMR structure deposited as PDB 4X8Y, and the amino acid residues used to obtain crystal and NMR structures [ 4 ]. (oncotarget.com)

These roles could potentially be reciprocally regulated by phosphorylation/dephosphorylation at Y113. (oncotarget.com)

Methylation of specific lysine residues in H3 and H4 causes further condensation of DNA around histones, and thereby prevents binding of transcription factors to the DNA that lead to gene repression. (wikipedia.org)
Well characterized modifications to histones include: Methylation Both lysine and arginine residues are known to be methylated. (wikipedia.org)

Predicted regulatory interaction sites for SH2- and SH3-domain proteins are in non-structured regions that could be available to cytoplasmic enzymes. (oncotarget.com)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
Finally, the C-terminus of HIPK1-3 comprises a region rich in serine, glutamine, and alanine (SQA) residues, which is involved in the interaction with different co-factors 14 . (nature.com)
These observations suggest that the stabilization of BChE tetramers is mediated through the interaction of the seven conserved aromatic residues, Trp 543, Phe 547, Trp 550, Tyr 553, Trp 557, Phe 561, and Tyr 564, and that the poly-L-proline induced increase in tetrameric BChE is mediated through these seven aromatic residues. (inrae.fr)

According to the histone code theory, PTMs recruit regulatory proteins or block their access to chromatin. (edu.sa)
Phosphorylation Ubiquitination However, there are many more histone modifications, and sensitive mass spectrometry approaches have recently greatly expanded the catalog. (wikipedia.org)
Analysis of CBP and p300 mutant mouse fibroblasts reveals CBP/p300 are together chiefly responsible for the global acetylation of histone H3 residues K18 and K27, and contribute to other locus-specific histone acetylation events. (aging-us.com)
Histone PTMs often correlate with the activity of the gene (or of a regulatory element such as an enhancer) that is in their proximity, suggesting a causal relationship. (aging-us.com)
For instance, a systematic analysis of 486 different histone H3 and H4 mutations in yeast (where every residue was mutated at least one way) showed that only 11 of 79 N-terminal tail deletions resulted in lethality, a phenotype that also depends to some extent on strain background [ 9 ]. (aging-us.com)

Chromatin remodeling is the dynamic modification of chromatin architecture to allow access of condensed genomic DNA to the regulatory transcription machinery proteins, and thereby control gene expression. (wikipedia.org)
Such condensed structure occludes many DNA regulatory regions, not allowing them to interact with transcriptional machinery proteins and regulate gene expression. (wikipedia.org)
They are regulatory proteins that play a role in G-protein-coupled receptor densensitization. (lookformedical.com)

Thus, we investigated changes in P2X3 receptor distribution in the lipid raft membrane compartment, their phosphorylation state, as well as their function with patch clamping. (biomedcentral.com)
Anantin application caused preferential P2X3 receptor redistribution to the lipid raft compartment and decreased P2X3 serine phosphorylation, two phenomena that were not interdependent. (biomedcentral.com)
Perilipin 5 S155 phosphorylation by PKA is required for the control of hepatic lipid metabolism and glycemic control. (uci.edu)
Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
We detected phosphorylation on S155 and identified S155 as a functionally important site for lipid metabolism. (uci.edu)

Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments. (biomedcentral.com)
To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammalian target of rapamycin (mTOR/FRAP), represents one of these pathways, we have examined the effect of simultaneous inhibition of the Ras-MAPK and PI3K-mTOR pathways on transformation of CEF by v-Src. (embl.de)

Here, we show that NEMO phosphorylation by GSK-3β leads to NEMO localization into multivesicular bodies (MVBs). (mdpi.com)

The familial dilated cardiomyopathy cTnC G159D mutation whose Ca2+ sensitivity is not modulated by cTnI phosphorylation exhibits a structure inherently more rigid than the wild type, with phosphorylation reversing the direction of all metrics relative to the wild type. (bvsalud.org)
Serine-to-alanine mutation at position 53 of the Kv7.5 amino terminus abrogated its ability to confer forskolin sensitivity to Kv7.4. (aspetjournals.org)

Phosphorylation levels of troponin I (TnI) and myosin binding protein-C (MyBP-C) were manipulated using protein kinase A and λ phosphatase. (bvsalud.org)
This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. (lookformedical.com)

This transition probably involves abnormal regulatory mechanisms, leading to excessive, prolonged and widespread MMP activity. (nature.com)

Moreover, a C-terminally adjacent autoinhibitory domain (AID) (935-1050) was identified in HIPK2, based on the observation that its removal increases phosphorylation activity 13 . (nature.com)

Further to the published interpretation, we suggest that phosphorylation of PGRMC1 at position Y113 may promote the attested membrane trafficking function of PGRMC1. (oncotarget.com)
In addition, I will discuss recent studies demonstrating that SphK1 is recruited to sphingosine-enriched endocytic vesicles and that phosphorylation of sphingosine to S1P by SphK1 is involved in endocytic membrane trafficking and autophagy and in the crosstalk between endocytosis and autophagy. (sphingolipidclub.com)

However, simultaneous inhibition of signaling by the Ras-MAPK pathway and the PI3K-mTOR pathway essentially blocked transformation. (embl.de)

We find that when present on thin filaments, phosphorylated Ser23/24 along with adjacent polar TnI residues interact closely with both tropomyosin and the N-lobe of TnC during our simulations. (bvsalud.org)

We describe here how the PGRMC1 structure also enables important new insights into the possible regulation of PGRMC1 function by phosphorylation. (oncotarget.com)

PKA phosphorylation affects troponin dynamics. (bvsalud.org)
Considering that each microRNA regulates up to hundreds of different mRNAs, and that each mRNA is regulated by tens of microRNAs, this finding adds a new layer of complexity to the regulatory dynamics of the human transcriptome. (pharmaceuticalintelligence.com)

Oxidative stress can have effects that are both up- and down-regulatory on insulin signalling. (biomedcentral.com)

We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. (uci.edu)
Expression of phosphorylation-defective PLIN5 S155A in Plin5 null cells resulted in decreased rates of lipolysis and triglyceride-derived fatty acid oxidation. (uci.edu)

Our observations provide new insights into the many functions of H3S10 phosphorylation. (edu.sa)

It follows that the phosphorylation status of PGRMC1 should be further explored in order to better understand many of its proposed biological functions. (oncotarget.com)

TAp63α is kept in an inactive and exclusively dimeric state but undergoes rapid phosphorylation-induced tetramerization and concomitant activation upon detection of DNA damage. (elifesciences.org)

An additional H3-derived bait containing the nonhydrolyzable phospho-serine mimic phosphonomethylen-alanine (Pma) at S10 recruited several isoforms of the 14-3-3 family and blocked the recruitment of HAT1 and RBBP7 to the unmodified H3-tail. (edu.sa)

Therefore, future studies on E-cadherin's positive regulatory role in TRAIL-induced apoptosis are warranted. (eurekaselect.com)
It mediates light-dependent PHOSPHORYLATION of RHODOPSIN and plays an important role in PHOTOTRANSDUCTION. (lookformedical.com)

Here, we report a general strategy for simultaneous analysis of both of these effects based on a SILAC MS scheme. (edu.sa)

Anatomy24

Organisms10

Chemicals and Drugs143

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes57

Information Science3

Threonine residues2

Oxidative phosphorylation in mitochondria2

Include acetylation2

Peptide8

Carboxy terminus1

Receptors4

Kinases3

Amino acid3

Dephosphorylation1

Methylation2

Interaction4

Histone5

Proteins3

Lipid5

Pathways2

Localization1

Mutation2

Protein4

Mechanisms1

Increases1

Membrane2

Pathway1

Interact1

Regulation1

Dynamics2

Insulin1

Cells2

Insights1

Biological1

Activation1

Family1

Role2

Analysis1