AdipocytesPreadipocytesLipidDifferentiationAccelerated the cell viabilityPhosphorylationApoptosisViabilityProliferationInhibitory effectObeseUpregulationConcentrationsDexamethasoneAMPKTriglyceridesMechanismsPathwaysExpressionPPARLipopolysaccharideInducesActivationSecretionMatureMitochondrialMiceHypertrophyPancreaticTreatmentDecreaseEffectsAdipose cellsActivityDifferentiateTerminalReducesLiverPrimary adipocyteMolecularVitroBiol
Adipocytes23
  • RESEARCH DESIGN AND METHODS AND RESULTS- First, we examined whether the PPARβ/δ agonist GW501516 prevents lipopolysaccharide (LPS)-induced cytokine production in differentiated 3T3-L1 adipocytes. (diabetesjournals.org)
  • KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to differentiate into adipocytes. (elsevierpure.com)
  • Taken together, these results suggest that PN suppresses C/EBP-δ expression by inhibiting Dex-induced GR promoter activity at the early stage of differentiation and, consequently, delays differentiation into mature adipocytes. (elsevierpure.com)
  • Wang et al ( 11 ) have illustrated curcumin's ability to reduce pro-inflammatory cytokines in 3T3-L1 adipocytes with FFA-induced insulin resistance. (spandidos-publications.com)
  • Thus, in both differentiating and differentiated 3T3-L1 adipocytes, high concentrations of melatonin increased intracytoplasmic triglyceride accumulation, an indicator of adipogenic differentiation. (unican.es)
  • These findings suggest that, in human breast tumors, melatonin could stimulate the differentiation of fibroblasts and reduce the aromatase activity and expression in both fibroblasts and adipocytes, thereby reducing the number of estrogen-producing cells proximal to malignant cells. (unican.es)
  • Results The consequences of L. cladonioides on cell viability in cultured 3T3-L1 adipocytes To judge the consequences of LC over the development of 3T3-L1 adipocytes, the cells had been treated with escalating focus (5-500 g/mL) for 24, 48, and 72 hr (Fig. ABT-869 novel inhibtior 1). (bioshockinfinitereleasedate.com)
  • Treatment of LC with 5 g/mL (11.7% at 24 hr), 10 g/mL (14.8% at 48 hr and 7.3% at 72 hr) significantly decreased the cell viability of 3T3-L1 ABT-869 novel inhibtior adipocytes. (bioshockinfinitereleasedate.com)
  • The outcomes of our data demonstrate that LC medication dosage did not have got toxicity in TGFB1 LC treated (5-500 g/mL) 3T3-L1 adipocytes. (bioshockinfinitereleasedate.com)
  • Acer Tegmentosum Maxim Inhibits Adipogenesis in 3t3-l1 Adipocytes and " by Hang-Hee Cho, Soo-Jung Lee et al. (touro.edu)
  • Protein kinase B (Akt) and glycogen synthase kinase 3β (GSK3β) phosphorylation was also decreased by ATM treatment of 3T3-L1 adipocytes. (touro.edu)
  • Epididymal fat exhibited significantly larger adipocytes in the HFD group than it did in the ATM-treated group. (touro.edu)
  • In mature adipocytes, G and GS individually caused apoptosis, but combination of G plus GS significantly increased apoptosis, more than either compound alone. (pcom.edu)
  • These results suggest that combination of genistein and guggulsterone may exert anti-obesity effects by inhibiting adipogenesis and inducing apoptosis in adipocytes. (pcom.edu)
  • We investigated the effects of the estrogen receptor-α (ERα) and -β (ERβ) in the regulation of leptin, resistin, and adiponectin expression in 3T3-L1 adipocytes. (korea.ac.kr)
  • ERα induced leptin expression and ERβ inhibited its expression in 3T3-L1 adipocytes. (korea.ac.kr)
  • PBM-treated hypertrophic cells (1064 nm and 17.6 J/cm2 every day for 7 days following addition of PA) decreased the lipid levels in hypertrophic adipocytes, restored the GLUT4 protein expression and enhanced glucose transport. (google.com)
  • The current results indicated that PBMT inhibited FFA generation and release in insulin-resistant adipocytes and reduced plasma FFA levels in diabetic db/db mice and HFD-fed mice. (google.com)
  • 8] where Rab10 was found at GSVs purified from 3T3-L1 adipocytes with anti-GLUT4 antibody. (colinsbraincancer.com)
  • Adipocytes were differentiated from 3T3-L1 cells and treated with high glucose ( HG ), H 2 S slow-releasing compound GYY4137 or transfected with CSE siRNA. (sdlifesciences.com)
  • In 3T3-L1 adipocytes, both HG DMEM and CSE siRNA transfection increased the mRNA and protein of MMP14. (sdlifesciences.com)
  • The cells screen an exponential development stage until confluence can be reached, go through mitotic clonal enlargement (MCE) that allows a rise in the ultimate percentage of differentiated fats cells, and get into the terminal maturation procedure for acquiring all of the specific tools of adipocytes [7]. (euromedh2020.com)
  • In addition to the classic brown adipocytes, a different type of brown fat cells seems to exist in tissues where WAT predominates. (biomedcentral.com)
Preadipocytes7
  • Here, we demonstrate the molecular mechanism underlying the effect of KSS on the differentiation of mouse preadipocytes (3T3-L1 cells). (elsevierpure.com)
  • A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K. (oregonstate.edu)
  • Our objective was to determine the effects of 6,7,4'-THIF on adipogenesis in 3T3-L1 preadipocytes and elucidate the mechanisms of action involved. (oregonstate.edu)
  • Adipogenesis was stimulated in 3T3-L1 preadipocytes. (oregonstate.edu)
  • Our results suggest that 6,7,4'-THIF suppresses adipogenesis in 3T3-L1 preadipocytes by directly targeting PI3K. (oregonstate.edu)
  • jujubarepresses adipogenesis in 3T3-L1 preadipocytes [16]. (euromedh2020.com)
  • The expression levels of LPL (lipoprotein lipase), HSL (Hormone-sensitive lipase), FAS (fatty acid synthase), and PPARα/PPARγ (peroxisome proliferator-activated receptor alpha/gamma) were significantly changed in the sheep preadipocytes during differentiation for 7 days. (academic-accelerator.com)
Lipid7
  • Risperidone increases adipocyte lipid accumulation by plausibly inhibiting long-chain fatty acid β-oxidation through targeting MAPK14 and MAPK8. (frontiersin.org)
  • 6,7,4'-THIF significantly suppressed MDI-induced lipid accumulation in the early stage of adipogenesis, attributable to a suppression of cell proliferation and the induction of cell cycle arrest. (oregonstate.edu)
  • Cellular lipid content in DMI (dexamethasone, 3-isobutyl-1-methylxanthine, and insulin mixture)-treated cells increased, while ATM treatment caused a significant reduction in lipid accumulation in differentiated 3T3-L1 cells. (touro.edu)
  • Notoginsenosides can treat obesity by reducing lipid synthesis, inhibiting adipogenesis, promoting white adipose tissue browning, increasing energy consumption, and improving insulin sensitivity. (frontiersin.org)
  • 2017. Novel acylated flavonol tetraglycoside with inhibitory effect on lipid accumulation in 3T3-L1 cells from Lu'an GuaPian tea and quantification of flavonoid glycosides in six major processing types of tea. (itjfs.com)
  • The attenuation of metal/peroxide oxidative chemistry, as well as lipid peroxidation, appears to be the major chemical mechanisms by which NO may limit oxidative injury to mammalian cells. (researchgate.net)
  • Electric pulse stimulation inhibited lipid accumulation on C2C12 myotubes incubated with oleic acid and palmitic acid. (academic-accelerator.com)
Differentiation6
  • MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
  • We also found that MC and PN were responsible for the anti-adipogenetic effect of KSS and significantly suppressed the expression of CCAAT/enhancer-binding proteins-δ (C/EBP-δ) mRNA 3 days after the induction of differentiation. (elsevierpure.com)
  • Silibinin inhibits adipocyte differentiation through a potential up-regulation of insig-1 and insig-2 at an early phase in adipocyte differentiation. (wikipedia.org)
  • The presence of melatonin during differentiation also induced a parallel reduction in aromatase expression and activity and expression of the cells. (unican.es)
  • We investigated the effect of Acer tegmentosum Maxim (ATM) on adipocyte differentiation in 3T3-L1 cells and anti-obesity properties in obese rats fed a high-fat diet (HFD). (touro.edu)
  • We conclude that by manipulating the composition of the culture medium it is possible to induce canine ADMSCs into insulin-producing cells, and the 2-staged protocol that was used promoted the best differentiation. (sdlifesciences.com)
Accelerated the cell viability1
  • RESULTS: In this study, the expression of RBM3, rather than CIRP, was altered in the skin of diabetic specimens, and the RBM3's overexpression accelerated the cell viability and proliferation of HaCaT cells under high glucose conditions. (bvsalud.org)
Phosphorylation2
  • Intersecting analysis, molecular docking, and pathway validation analysis showed that risperidone influences the adipocytokine signaling pathway by targeting MAPK14 (mitogen-activated protein kinase 14), MAPK8 (mitogen-activated protein kinase 8), and RXRA (retinoic acid receptor RXR-alpha), thereby inhibiting long-chain fatty acid β-oxidation by decreasing STAT3 (signal transducer and activator of transcription 3) expression and phosphorylation. (frontiersin.org)
  • Risperidone inhibits leptin-mediated phosphorylation of STAT3-Y705, leading to weight gain ( 26 ). (frontiersin.org)
Apoptosis7
  • Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
  • Liver cell apoptosis is a prominent feature of NAFLD and correlates with disease severity ( 2 ). (spandidos-publications.com)
  • In the present study, we investigated the effects of combinations of G plus GS on apoptosis and adipogenesis in 3T3-L1 cells. (pcom.edu)
  • KR could increase the growth of H9c2 inhibited by LPS, reduce apoptosis rate, and reverse the elevated levels of IL-1β, IL-6, and TNF-α. (itjfs.com)
  • Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
  • In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
  • These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Viability4
  • Cell viability was analyzed by Cell-Counting Kit-8 assay and colony formation assay. (bvsalud.org)
  • LC treatment inhibited cell viability within a dosage dependent manner. (bioshockinfinitereleasedate.com)
  • Open up in another screen Fig. 1 The result of on cell viability in 3T3-L1 cells. (bioshockinfinitereleasedate.com)
  • After that, HTR-8/SVneo cells (human trophoblasts) were chosen and they were subjected to δ-tocotrienol treatment and then Cell Counting Kit-8 was used to test cell viability. (tocotrienolresearch.org)
Proliferation2
  • High-glucose Dulbecco's modified Eagle medium ( HG - DMEM ) supplemented with 20% fetal bovine serum followed by HG - DMEM with 2% horse serum is found to enable proliferation of both C2C12 muscle precursor cells and TE85 human osteosarcoma cells, fusion of C2C12s into myotubes, as well as an upregulation of RUNX2/CBFa1 in TE85s. (sdlifesciences.com)
  • H9c2 cell proliferation was detected by cell counting kit -8 assay. (itjfs.com)
Inhibitory effect1
  • Even though inhibitory effect is definitely minor it is noteworthy that a model assigning GSV formation in the TGN does not imply that the TGN-localized GLUT4 would be directly translocated to the cell surface in response to insulin activation. (colinsbraincancer.com)
Obese1
  • The aim of this study was to research whether a water extract of (LC) comes with an anti-obesity effect in 3T3-L1 cells and obese mice. (bioshockinfinitereleasedate.com)
Upregulation1
  • The addition of GYY4137 inhibited HG -induced upregulation of MMP14 expression. (sdlifesciences.com)
Concentrations5
  • METHODS: In vitro experiments, western blot assay was used to test the levels of proteins in HaCaT cells treated with different concentrations of glucose. (bvsalud.org)
  • The migration of HaCaT cells at different concentrations of glucose was evaluated by wound healing assay. (bvsalud.org)
  • Differentiated 3T3-L1 cells had been incubated with on the indicated several concentrations for 24, 48, and 72 hr. (bioshockinfinitereleasedate.com)
  • There are a number of reactive nitrogen oxide species, such as N2O3 and ONOO-, that can alter critical cellular components under high local concentrations of NO. However, NO can also abate the oxidation chemistry mediated by reactive oxygen species such as H2O2 and O2- that occurs at physiological levels of NO. In addition to the antioxidant chemistry, NO protects against cell death mediated by H2O2, alkylhydroperoxides, and xanthine oxidase. (researchgate.net)
  • Concentrations of cholesterol and triglycerides are strongly correlated in the adipocyte, but little is known about mechanisms regulating cholesterol metabolism in fat cells. (jci.org)
Dexamethasone1
  • In addition, PN inhibited dexamethasone (Dex)-induced glucocorticoid receptor (GR) promoter activity. (elsevierpure.com)
AMPK4
  • AMPK pathways inhibit biosynthetic pathways with energy consumption, such as protein, fatty acid and glycogen synthesis. (springer.com)
  • Furthermore, KR could suppress the expression levels of AMPK and SIRT1, and AMPK-specific inhibitor (Compound C) could significantly counteract the activation of KR, indicating that its anti-inflammatory effect is achieved by regulating the AMPK/SIRT1 signaling pathway. (itjfs.com)
  • 2022. Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation. (itjfs.com)
  • 2018. Activation of AMPK inhibits inflammatory response during hypoxia and reoxygenation through modulating JNK-mediated NF-κB pathway. (itjfs.com)
Triglycerides1
  • by inhibiting the hydrolysis of triglycerides, Orlistat reduces the absorption of fat from food to achieve weight loss. (frontiersin.org)
Mechanisms2
  • 16] Potential mechanisms through which TCF7L2 variants influence T2DM include its role in adipogenesis, myogenesis, and pancreatic islet development, as well as in beta-cell survival and insulin secretory granule function. (medscape.com)
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Pathways2
  • NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
  • Abstract: Melatonin inhibits the genesis and growth of breast cancer by interfering at different levels in the estrogen-signaling pathways. (unican.es)
Expression12
  • The effects on dopamine (DA) release and dopamine transporter (DAT) gene expression were assayed in hypothalamic HypoE22 cells. (researchgate.net)
  • In the same experimental system, IL-10 and BDNF gene expressions increased, and in HypoE22 cells, the extract decreased the extracellular dopamine level and increased the DAT gene expression due to the direct interaction of parthenolide with the DAT. (researchgate.net)
  • 1,25-(OH)2D3 transiently but strongly induces Insig-2 expression in 3T3-L1 cells. (wikipedia.org)
  • Melatonin inhibits aromatase activity and expression in human breast cancer cells, thus behaving as a selective estrogen enzyme modulator. (unican.es)
  • We observed that 6,7,4'-THIF, but not daidzein, inhibited MDI-induced adipogenesis significantly at 40 and 80 μM, associated with decreased peroxisome proliferator-activated receptor-γ and C/EBP-α protein expression. (oregonstate.edu)
  • Since these events occur at a distance from the cell body, post-transcriptional control of gene expression has strategic advantage to fine-tune the overall regulation of protein contents in situ. (koreascience.kr)
  • Differential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease. (springer.com)
  • To clarify the expression and regulation of adipokines by ER subtypes, total RNA was extracted from cells and measured using quantitative PCR. (korea.ac.kr)
  • Photoactivation of AKT inhibited the transcriptional activity of Forkhead box transcription factor O1 (FoxO1), reducing expression of lipolytic enzymes and FFA generation and release. (google.com)
  • Increased OLR1 expression, resulting either from TZD treatment or adenoviral gene delivery, significantly augments adipocyte cholesterol content and enhances fatty acid uptake. (jci.org)
  • Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
  • Maturation of BAT and WAT follow a similar adipogenic transcriptional program, albeit several genes show cell type-dependent expression. (biomedcentral.com)
PPAR1
  • ATM (60 ug/mL) caused inhibition of adipogenesis via down-regulation of the CCAAT/enhancer binding protein β (C/EBPβ) (48%), C/EBPα (66%), and peroxisome proliferator-activated receptor γ (PPARγ) (64%) expressions in 3T3-L1 cells. (touro.edu)
Lipopolysaccharide1
  • The inflammatory injury model of H9c2 cells was established by lipopolysaccharide (LPS) induction. (itjfs.com)
Induces1
  • The actived caspase-1 also induces a proinflammatory form of cell death [ 12 ]. (ijbs.com)
Activation2
  • These recent studies showed that curcumin inhibited HSCs activation, and suppressed hepatic fibrogenesis in vitro and in vivo ( 6 , 7 ). (spandidos-publications.com)
  • PBMT promoted mitochondrial reactive oxygen species (ROS) generation, which inhibited phosphatase and tensin homologue (PTEN) and promoted protein kinase B (AKT) activation. (google.com)
Secretion2
  • Finally, TCF7L2 polymorphisms have been associated with impaired insulin secretion, glucose production, and glucose tolerance via direct effects on pancreatic islet beta cells. (medscape.com)
  • Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
Mature3
  • Although the majority of works on FMRP have been performed in neurons, it is also found in the developing or mature glial cells including oligodendrocytes, where its function is not well understood. (koreascience.kr)
  • Mature IL-1β is an effective proinflammatory mediator in many immune responses, including the recruitment of natural immune cells to the infection site and the regulation of adaptive immune cells. (ijbs.com)
  • Mature IL-18 plays an important role in the production of IFN-γand the enhancing the cytolytic activity of natural killer cells and T cells [ 11 ]. (ijbs.com)
Mitochondrial2
  • In addition, curcumin contributed to cell survival, as indicated by the restoration of the mitochondrial membrane potential (MMP) and the inhibition of the mitochondrial biogenesis induced by HFFA. (spandidos-publications.com)
  • There is also growing evidence that FFA-mediated oxidative stress contributes significantly to mitochondrial dysfunction in the liver. (spandidos-publications.com)
Mice1
  • The adzuki bean paste showed potential hypoglycemic activity in both normal mice and streptozotocin (STZ)-induced diabetic rats after an oral administration of sucrose, but did not show any effect on the blood glucose concentration after glucose administration, suggesting that the active fraction suppressed the postprandial blood glucose level by inhibiting alpha-glucosidase and alpha-amylase, irrespective of the endogenous blood insulin level. (healthjade.com)
Hypertrophy1
  • Enlargement of adipose cells is connected with hyperplasia, a rise in cellular number, or hypertrophy, a rise in cell size [4]. (euromedh2020.com)
Pancreatic2
  • Curcumin also exerts potential anti-inflammatory effects in diverse cell types, including pancreatic cells, chondrocytes, and hepatic cells ( 9 , 10 ). (spandidos-publications.com)
  • Type I diabetes, that is called insulin-dependent diabetes mellitus (IDDM) or juvenile-onset diabetes develops when the body's immune system destroys pancreatic β-cells, the only cells in the body that produce the hormone insulin that regulates blood glucose. (gotomydoctor.com)
Treatment2
  • Curcumin treatment inhibited the lipoapoptosis, ROS production and ATP depletion elicited by HFFA in primary hepatocytes. (spandidos-publications.com)
  • Although earlier studies using BFA to interfere with TGN function gave ambiguous results about the part of the TGN in GLUT4 translocation [23-26] in the present study it was found that BFA treatment partially inhibits insulin-stimulated GLUT4 translocation (Number 3). (colinsbraincancer.com)
Decrease1
  • MCHR2 has been observed to significantly decrease INSIG2. (wikipedia.org)
Effects1
  • Eliminating ROS elimination or inhibiting AKT blocked the effects of the laser therapy in vivo and in vitro. (google.com)
Adipose cells1
  • Weight problems can be an immoderate build up of adipose cells, an organ made up of fats cells. (euromedh2020.com)
Activity1
  • 6,7,4'-THIF was found to inhibit PI3K activity via direct binding in an ATP-competitive manner. (oregonstate.edu)
Differentiate1
  • The aim of this study was to differentiate canine adipose-derived mesenchymal stem cells (ADMSCs) into insulin-producing cells by using culture media with different compositions to determine the most efficient media. (sdlifesciences.com)
Terminal1
  • two main regulators of terminal adipogenesis, in 3T3-L1 cells. (unican.es)
Reduces1
  • Lactobacillus plantarum TN627 significantly reduces complications of alloxan-induced diabetes in rats. (springer.com)
Liver1
  • 17) have shown recently that a water-soluble extract of the adzuki beans could inhibit acetaminophen-induced liver damage. (healthjade.com)
Primary adipocyte2
  • 17, 18] TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and in primary adipocyte stem cells. (medscape.com)
  • When comparing primary adipocyte profiles, with those of cell lines reported in the literature, we found a high degree of difference in 'adipogenesis' regulated miRNAs suggesting that the model systems may not be accurately representing adipogenesis. (biomedcentral.com)
Molecular2
  • 3T3-L1 cells are utilized extensively like a cell tradition model to review the molecular control of adipogenesis [5]. (euromedh2020.com)
  • Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. (deepdyve.com)
Vitro2
  • Several models exist to study adipogenesis in vitro , of which the cell line 3T3-L1 is the most well known, albeit not the most physiologically appropriate. (biomedcentral.com)
  • In conclusion, we provide a preliminary analysis of miRNAs associated with primary cell in vitro adipogenesis and demonstrate that the inflammation-associated miRNA, mir-21 is up-regulated in subcutaneous adipose tissue in human obesity. (biomedcentral.com)
Biol1