Elevated in aging skeletalDifferentiationMyoDRegenerationPorcineProliferationMyofiberGeneQuiescent satellite cellsPromotesTissues2022Progenitor cellsPrecursorReceptorPostnatalMyonucleiNuclearFibroblastRolesExperimentsImmunohistochemistryOccurCellularStem cellsPrimaryAminoActivateRoleCycleResultsEffectsHealthy
Elevated in aging skeletal1
- MyoD mRNA levels are also reported to be elevated in aging skeletal muscle. (wikipedia.org)
Differentiation15
- These bHLH (basic helix loop helix) transcription factors act sequentially in myogenic differentiation. (wikipedia.org)
- high MyoD expression represses cell renewal, promotes terminal differentiation and can induce apoptosis. (wikipedia.org)
- The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
- Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)
- CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)
- When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
- Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)
- Long non-coding RNAs (lncRNAs) are involved in the process of muscle cell differentiation and play an important role. (bvsalud.org)
- Previous studies have shown that lncRNA-MEG3 promotes the differentiation of porcine skeletal muscle satellite cells (PSCs), but the regulatory mechanism of MEG3 interaction with target protein has not been well studied. (bvsalud.org)
- Furthermore, we have successfully predicted and validated that the transcription factor myogenic differentiation (MYOD) binds to the MEG3 core promoter though utilizing chromatin immunoprecipitation (CHIP) and luciferase reporter assays. (bvsalud.org)
- The bHLH transcription factor MyoD is a master regulator of myogenic differentiation, and its sustained expression in fibroblasts suffices to differentiate them into muscle cells. (bvsalud.org)
- Unstable MyoD oscillations and long periods of sustained MyoD expression are observed when stem cells undergo myogenic differentiation. (bvsalud.org)
- Thus, oscillations of MyoD and Hes1 control the balance between the proliferation and differentiation of muscle stem cells. (bvsalud.org)
- Stephane Koenig ABSTRACT Ca 2+ signaling plays a key role during human myoblast differentiation. (biologists.com)
- Using synchronized cultures of C2C12 myoblasts to model these divergent programs, we show that p8 (also known as Nupr1 ), a G1-induced gene, negatively regulates the cell cycle and promotes myogenic differentiation. (biologists.com)
MyoD23
- MyoD, which was discovered in the laboratory of Harold M. Weintraub, belongs to a family of proteins known as myogenic regulatory factors (MRFs). (wikipedia.org)
- MyoD is one of the earliest markers of myogenic commitment. (wikipedia.org)
- MyoD is expressed at extremely low and essentially undetectable levels in quiescent satellite cells, but expression of MyoD is activated in response to exercise or muscle tissue damage. (wikipedia.org)
- MyoD was cloned by a functional assay for muscle formation reported in Cell in 1987 by Davis, Weintraub, and Lassar. (wikipedia.org)
- The researchers expressed the complementary DNA (cDNA) of the murine MyoD protein in a different cell lines (fibroblast and adipoblast) and found MyoD converted them to myogenic cells. (wikipedia.org)
- The function of MyoD in development is to commit mesoderm cells to a skeletal myoblast lineage, and then to regulate that continued state. (wikipedia.org)
- One of the main actions of MyoD is to remove cells from the cell cycle (halt proliferation for terminal cell cycle arrest in differentiated myocytes) by enhancing the transcription of p21 and myogenin. (wikipedia.org)
- Thus MyoD enhances its own activity in the cell in a feedforward manner. (wikipedia.org)
- MyoD is a transcription factor and can also direct chromatin remodelling through binding to a DNA motif known as the E-box. (wikipedia.org)
- KAP1 is localized at muscle-related genes in myoblasts along with both MyoD and Mef2 (a myocyte transcription enhancer factor). (wikipedia.org)
- When the kinase MSK1 phosphorylates KAP1, the corepressors previously bound to the scaffold are released allowing MyoD and Mef2 to activate transcription. (wikipedia.org)
- Once the "master controller" MyoD has become active, SETDB1 is required to maintain MyoD expression within the cell. (wikipedia.org)
- MyoD has also been shown to function cooperatively with the tumor suppressor gene, Retinoblastoma (pRb) to cause cell cycle arrest in the terminally differentiated myoblasts. (wikipedia.org)
- In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
- The results indicated that MYOD acts as a transcription factor of MEG3 to promote MEG3 transcription. (bvsalud.org)
- It is concluded that MYOD acts as a transcription factor to induce MEG3 expression. (bvsalud.org)
- MyoD expression oscillates in activated muscle stem cells of developing, postnatal and adult muscle under various conditions: when the stem cells are dispersed in culture, when they remain associated with single muscle fibers, or when they reside in muscle biopsies. (bvsalud.org)
- The oscillatory expression of MyoD is driven by the oscillatory expression of the bHLH transcription factor Hes1 that periodically represses MyoD. (bvsalud.org)
- Here, we describe time-lapse imaging methods using luciferase reporters, which can monitor dynamic MyoD gene expression in myogenic cells. (bvsalud.org)
- Here, we examined the time-dependent expression patterns of multiple biomarkers associated with satellite cell fate, including the transcription factor paired box 7 (Pax7), myoblast determination protein (MyoD), myogenin, and insulin-like growth factor (IGF-1), using immunohistochemistry, western blotting, and quantitative real-time PCR in contused skeletal muscle. (bvsalud.org)
- Morphometrically, the data obtained from the numbers of Pax7 + , MyoD + , and myogenin + cells were highly correlated with the wound age. (bvsalud.org)
- Pax7, MyoD, myogenin, and IGF-1 expression patterns were upregulated after injury at both the mRNA and protein levels. (bvsalud.org)
- Pax7, MyoD, and myogenin protein expression levels confirmed the results of the morphometrical analysis. (bvsalud.org)
Regeneration9
- Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
- After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
- These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
- 1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
- 6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
- Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
- Knockdown of MEG3 in vivo indicated that MEG3 is involved in skeletal muscle regeneration. (bvsalud.org)
- From the perspective of forensic wound age estimation, experiments related to skeletal muscle regeneration after injury have rarely been reported. (bvsalud.org)
- Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)
Porcine1
- Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
Proliferation6
- Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
- Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
- Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
- A single, early local administration of high-dose bFGF prevented atrophic changes in the thyroarytenoid muscles by activating satellite cell proliferation and reforming neuromuscular junctions. (biomedcentral.com)
- WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
- Hepatocyte growth factor (HGF) is produced by stromal and mesenchymal cells, and it stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its cognate receptor, Met. (spandidos-publications.com)
Myofiber2
- Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
- There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
Gene3
- Various growth factors, cytokines, and prostaglandins upregulate HGF gene expression, including basic fibroblast growth factor, oncostatin M, hypoxia-inducible factor 1α and nuclear factor-κB (NF-κB) ( 9 ). (spandidos-publications.com)
- By contrast, transforming growth factor (TGF)-β1 was demonstrated to markedly downregulate HGF gene expression ( 10 , 11 ). (spandidos-publications.com)
- Here, we find that persistent mtDNA stress is not associated with basally activated NF-κB signalling or interferon gene expression typical of an acute antiviral response. (regenerativemedicine.net)
Quiescent satellite cells1
- 10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
Promotes2
- The HGF‑Met signaling pathway contributes in a paracrine manner to the development of epithelial organs, exerts regenerative effects on the epithelium, and promotes the regression of fibrosis in numerous organs. (spandidos-publications.com)
- HGF is produced and secreted by adjacent stromal and mesenchymal cells, it contributes to the development of epithelial organs in a paracrine fashion, exerts regenerative effects on epithelia in the liver, kidney, lung, and other tissues, and promotes the regression of fibrosis in numerous organs ( 7 , 8 ). (spandidos-publications.com)
Tissues3
- Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
- Jyotsna Dhawan ABSTRACT Adult stem cells persist in mammalian tissues by entering a state of reversible quiescence, referred to as G 0 , which is associated with low levels of transcription. (biologists.com)
- Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
20221
- J Cell Sci (2022) 135 (15): jcs259789. (biologists.com)
Progenitor cells1
- Adult regenerative myogenesis is dependent on progenitor cells. (biologists.com)
Precursor1
- Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
Receptor1
- Roles for Eph receptor tyrosine kinase signaling in the formation of topographic patterns of axonal connectivity have been well established in vertebrate visual systems. (sdbonline.org)
Postnatal1
- The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)
Myonuclei1
- Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
Nuclear1
- Nuclear factor of activated T-cell (NFAT. (biologists.com)
Fibroblast3
- Research on regenerative medicine using basic fibroblast growth factor (bFGF) has recently advanced in the field of laryngology. (biomedcentral.com)
- The basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family and is a key protein in regenerative medicine. (biomedcentral.com)
- HGF was cloned as a growth factor for hepatocytes ( 1 , 2 ), is identical to scatter factor (SF) and was originally discovered as a fibroblast-derived cell motility factor for epithelial cells ( 3 ). (spandidos-publications.com)
Roles1
- Studies targeted at establishing such effects at cellular level and their correlations with in vivo models, will broaden our understanding of the roles played by androgens on skeletal muscle function in elderly. (frontiersin.org)
Experiments1
- Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
Immunohistochemistry1
- Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)
Occur1
- Such lack of clarity can be attributed to difficulty in isolating the individual events responsible for alterations in skeletal muscle, most of which occur simultaneously, among the multiple age-associated changes and co-morbidities associated with advanced age. (frontiersin.org)
Cellular1
- We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)
Stem cells1
- This interferes with the maintenance of activated muscle stem cells and impairs muscle growth and repair. (bvsalud.org)
Primary2
- This age-related decline in skeletal muscle mass and strength generation, the primary function of skeletal muscle mass, is known as sarcopenia ( 2 , 3 ). (frontiersin.org)
- In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
Amino3
- Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
- Therefore, amino acids in multifunctional docking sites of Met have been exchanged with specific binding motifs for downstream adaptor molecules in order to investigate the signaling potential of the HGF‑Met signaling pathway. (spandidos-publications.com)
- It encodes the inactive pre-pro-HGF, a single chain of 728 amino acids (83 kDa), which includes a signal sequence ( 1 - 31 ), a heavy α chain (69 kDa), and a light β chain (34 kDa). (spandidos-publications.com)
Activate1
- 7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)
Role1
- These results demonstrate a role of Eph/ephrin signalling in a range of behaviours affected in AD. (sdbonline.org)
Cycle1
- The oscillatory period is around 3 h and thus much shorter than the cell cycle or circadian rhythm. (bvsalud.org)
Results1
- The term sarcopenia was first proposed in 1989 by Irwin Rosenberg to describe a multifactorial syndrome that occurs with age and results in loss of skeletal muscle mass and function ( 3 , 4 ). (frontiersin.org)
Effects2
- However, most evidence linked testosterone and skeletal muscle effects are observational. (frontiersin.org)
- Utilising fly genetics, this study generated the first Drosophila model of human wild-type and P460L mutant EphA1 and tested the effects of Eph /ephrin signalling on AD-relevant behaviour and neurophysiology. (sdbonline.org)
Healthy1
- In healthy young adults, the skeletal muscle mass comprises approximately 60% of total body mass. (frontiersin.org)